24 results about "Mixed cell" patented technology

The application discloses a cell liquid subpackaging method, which is applied to a cell liquid subpackaging system. The cell liquid subpackaging system comprises a peristaltic pump, a subpackaging bag, a calibration container and a diaphragm pump which are sequentially connected in the extension direction of the same subpackaging pipeline. The subpackaging method pumps cell liquid into the subpackaging bag first, then discharges the mixed cell liquid and the residual gas in the subpackaging bag into the subpackaging pipeline through the backflow mode, discharges the residual gas mixed in the subpackaging pipeline through the calibration container and the diaphragm pump on the subpackaging pipeline, obtains the cell liquid after gas-liquid separation, and then pumps the cell liquid after gas-liquid separation into the subpackaging bag again, so that the subpackaging product without residual bubbles is obtained, and the subpackaging quality is improved.

The invention discloses a multilayer adherent cell culture reactor and a use method. The culture reactor comprises a shell, a plurality of layers of cell culture plates are arranged in the shell in an array manner; and the liquid inlet is used as an inlet of a cell suspension. The liquid outlet is an outlet of the culture medium; the air holes are formed in the upper surface and the lower surface of the shell. And the quality control chamber is communicated with the inner cavity of the shell. The use method comprises the following steps: uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for positive culture; after front culture is completed, the multi-layer adherent cell culture device is inverted; uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for reverse culture. By arranging the multiple layers of cell culture plates with double surfaces capable of culturing, the cell culture area is greatly increased, the space utilization rate is improved, the use amount of a culture medium is reduced, the prepared quality control chamber is communicated with the inner cavity of the shell, and the cell growth state is conveniently, rapidly and accurately observed on the premise of not polluting the internal environment and not damaging the culture stability through isolation of the partition opening.

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

The application provides a method for preparing FFPE quality control products by using cell lines, a reference product and a kit, and belongs to the fields of medicine, clinical laboratory science and biotechnology, and comprises the following steps: culturing cell lines by using microcarriers; mixing different cell lines based on requirements; centrifuging the mixed cell microcarrier suspension in a sealed malt pipe at one end; removing supernatant after the treatment; packaging the two ends of the malt pipe by using a semi-permeable membrane; removing the semi-permeable membrane after fixing, dehydrating and transparentizing the malt pipe by using a formaldehyde solution; separating the cell microcarriers after the wax immersion treatment from the malt pipe; and embedding the cell microcarriers into a paraffin block by using wax. The quality control product can be used in subsequent quality control processes, the mutation frequency of the quality control product can be kept in good uniformity, and batch production and large-scale production of the quality control product can be realized.

The present invention relates to a cell population of spiral ganglion neural progenitors (SGNPs) and methods how to isolate these cells from an in-vitro mixed cell population. The cell population expresses one or more markers, wherein the one or more marker is CXCR4, SUSD2, LPAR3, EPHA5, CHRNA3, REEP1, NPFFR2, SORCS3, NFASC, GABRB3, KCNB2, GRM8, DLL3, and / or KCNH8.

The invention discloses a method for efficiently and directionally inducing IPS to be differentiated into pancreatic beta cells. According to the method, through staged signal regulation and strict cell screening, the directionality and the overall efficiency of differentiation are remarkably improved. The whole process pays attention to cell quality control from the initial stage, and a stable foundation is laid for subsequent differentiation by screening iPS cells with uniform morphology and high expression of pluripotent markers. Progressive signal molecule regulation is adopted in each stage, and an accurate differentiation path is formed from Activin A gradient adjustment during endoderm induction, synergistic effect of FGF10 and retinoic acid in pancreatic precursor cell differentiation and Wnt signal inhibition during endocrine precursor cell induction, so that the non-specific differentiation tendency is effectively reduced. Meanwhile, cell enrichment steps of key nodes, such as SOX17 positive cell screening in an endoderm stage and Ngn3 magnetic bead sorting of endocrine precursor cells, further improve the purity of a target cell population and avoid interference of parenchyma cells on subsequent function maturation.

The invention provides a microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device, and relates to the technical field of microalgae cell screening and detection. The microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device comprises a signal processing unit, the substrate is ITO (Indium Tin Oxide) conductive glass; the main micro-channel is of a micro-channel structure which is formed in a polydimethylsiloxane (PDMS) layer and is bonded on the substrate. According to the invention, the passive sorting module based on the expansion and contraction inertia effect and the differential impedance detection module are integrated in the single micro-fluidic chip, so that the full-process automation of'sample introduction-sorting-detection-output 'is realized. Sample loss, cross contamination and activity damage are avoided, interference of mixed cell populations on subsequent impedance signals is effectively eliminated through pre-sorting, and the accuracy of target microalgae detection is remarkably improved.

The present disclosure provides a method of performing a functional assay on human spheroids, e.g., three-dimensional human cell spheroids using, in one embodiment, a fluorometric imaging plate reader.

The application discloses a cell multi-modal analysis system and method based on a micro-fluidic chip, and the system comprises a data acquisition and processing module, a cell classification module and a cell three-dimensional reconstruction module.

An energy efficient aquaculture system combining mixed-cell and raceway configurations. The system comprises a raceway tank, a raceway channel, a first water purification subsystem, and a second water purification subsystem. The system may include one or more of a hatching subsystem, a nursery subsystem, a feeding subsystem, a finishing subsystem, and a fish pumping system for transfer of fish between raceway tanks. A method of growing fish for commercial production using the aquaculture system is also provided.

The invention provides a method for preparing an FFPE quality control product by using a cell line, a reference product and a kit, and belongs to the technical field of medicine, clinical laboratory science and biologication.The method comprises the steps that the cell line is cultured by adopting a microcarrier; mixing different cell lines based on requirements; pouring the mixed cell microcarrier suspension into a straw with one sealed end, carrying out centrifugal treatment, removing the supernatant after the treatment is finished, then packaging the two ends of the straw by adopting semi-permeable membranes, carrying out formaldehyde solution fixation, dehydration and transparent treatment, removing the semi-permeable membranes, and carrying out wax dipping treatment; and separating the cell microcarrier subjected to wax dipping treatment from the straw, and then embedding the cell microcarrier with wax to form a paraffin block. The mutation frequency of the quality control product can keep good uniformity in the subsequent quality control process, and batch and large-scale production of the quality control product is achieved.

The embodiment of the invention provides a single cell sorting device. The single cell sorting device comprises a distributor, a mixer and an on-demand titrator, the distributor comprises a sample container and a leading-out device, a containing cavity is formed in the sample container, at least part of the cavity wall of the containing cavity is arranged to be a deformable part, the leading-out device is arranged below the sample container and connected to a bottom outlet of the containing cavity, the mixer is arranged relative to the sample container, and the on-demand titrator is arranged relative to the leading-out device; the use method of the single cell sorting device comprises the following steps: S1, adding a cell solution sample into the accommodating cavity from the input port of the accommodating cavity; and S2, starting the mixer and the on-demand titrator, applying an external force to the deformable part by the mixer so as to uniformly mix the cell solution in the accommodating cavity, enabling the uniformly mixed cell solution to enter the exporting device, and applying an external force to the exporting device by the on-demand titrator so as to export the cell solution from the exporting device in a liquid drop form. According to the embodiment of the invention, the technical effects of relatively high automation degree and prevention of pollution caused by manual intervention are achieved.

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

Materials and methods for labeling and isolating particular cell types from mixed cell populations are provided herein. Also provided herein are methods for generating data representing a synthetic genetic sequence configured for labeling at least one cell type by causing expression of a marker in the at least one cell type.

Semiconductor structures are provided. A semiconductor structure includes a cell array. The cell array includes a first regular cell, a second regular cell and a first mixed cell. Each P-type transistor has a first threshold voltage and each N-type transistor has a second threshold voltage in the first regular cell. Each P-type transistor has a third threshold voltage and each N-type transistor has a fourth threshold voltage in the second regular cell. Each P-type transistor has the first threshold voltage and each N-type transistor has the fourth threshold voltage in the first mixed cell. The first regular cell, the second regular cell and the first mixed cell are arranged in the same row of the cell array. The first mixed cell is arranged between the first and second regular cells and is in contact with the first regular cell.

Provided herein are methods of enriching for photoreceptors and photoreceptor precursor cells from mixed cell populations. Such enriched populations of photoreceptors and photoreceptor precursor cells can be used as cell therapies for the treatment of retinal disorders.

The invention provides a construction method of a pleural effusion-derived tumor cell line of a lung adenocarcinoma patient, and belongs to the technical field of cell construction. The method comprises the following steps: carrying out single cell level accurate separation and purification on pleural effusion of a lung adenocarcinoma patient, carrying out cell type identification and tumor cell pre-enrichment in combination with a single cell transcriptome sequencing technology before primary culture, and carrying out incubation, subculture and subsequent accurate enrichment. And a tumor cell line which is highly enriched and has stable genetic characteristics is successfully obtained. The tumor cell line constructed by the method disclosed by the invention can be passaged for multiple times, so that individualized tumor antigen information and unique gene expression profiles of patients are reserved to the greatest extent, and meanwhile, the challenges of serious hybrid cell pollution, low tumor cell purity, low culture success rate and the like in the traditional method are overcome.

The invention relates to the technical field of subpackaging, and provides a cell preparation subpackaging method and system. The method comprises the following steps: controlling a uniform mixing device to uniformly mix a cell preparation in a uniform mixing bag to obtain a uniformly mixed cell preparation; and controlling an injection device to inject the uniformly mixed cell preparation, and sequentially sub-packaging the uniformly mixed cell preparation in the injection device into each sub-packaging bag according to a preset sub-packaging volume. The whole process depends on machine automatic control, so that low subpackaging efficiency and subpackaging precision caused by manual operation are avoided, meanwhile, the injection device is controlled to accurately subpackage the cell preparation with the preset subpackaging volume into each subpackaging bag, the situation that the volume of the cell preparation is controlled through manual weighing is avoided, and the subpackaging efficiency and subpackaging precision are improved. Therefore, the influence of vibration of pipelines and other devices on volume control is avoided, the subpackaging precision is further improved, and the treatment effect of the cell preparation and the treatment opportunity of a patient are not influenced.

The invention relates to a multifunctional cell element design method for additive manufacturing, belongs to the technical field of structural design, and particularly relates to the field of multifunctional cell element design subdivision for additive manufacturing. According to the method, the entity thickness of the TPMS curved surface structure can be adjusted by adjusting the level set parameters in the formula, and the relative density of cell elements is changed. According to the method, a function group of each cell configuration feature is established, Boolean operation summation and intersection operation of the structure is realized through a maximum value function and a minimum value function, and the complex mixed cell structure design is completed; according to the method disclosed by the invention, a design and characterization method of the mixed truss lattice and TPMS curved surface structure is established based on a topology description function method, and means and tools for researching the geometric structure of cell elements are expanded.

A cell preparation aliquoting method and system, the method comprises: controlling a mixing apparatus to perform a mixing operation on a cell preparation in a mixing bag to obtain a mixed cell preparation (101); controlling an injection apparatus to draw in the mixed cell preparation, and sequentially aliquoting the mixed cell preparation in the injection apparatus into each aliquot bag according to a preset aliquot volume (102). Since the entire process relies on automated machine control, it avoids low aliquoting efficiency and accuracy caused by manual operation. At the same time, controlling the injection apparatus to accurately aliquot the cell preparation into each aliquot bag according to the preset aliquot volume avoids using manual weighing for volume control, thereby avoiding the impact of vibrations from pipelines and other apparatuses on volume control, further improving aliquoting accuracy and ensuring that the therapeutic effect of the cell preparation and the timing of patient treatment are not affected.

A semiconductor device includes: first and second cell regions including corresponding active regions (ARs) in which are formed components of transistors. The first cell region includes first and second ARs having a first height The second cell region includes third and fourth ARs having a second height taller than the first height, and a fifth AR having a third height taller than the second height. The transistors of the first and second cell regions are arranged to function as a D flip-flop (DFF) including a primary latch and a secondary latch. The primary latch includes a first sleepy inverter and a first non-sleepy (NS) inverter. The secondary latch includes a second sleepy inverter and a second NS inverter. Transistors which comprise the first and second NS inverters are in the first cell region. Transistors which comprise the first and second sleepy inverters are in the second cell region.

The present disclosure provides systems and methods for analyzing a mixed population of cells. In particular, the present disclosure provides systems and methods for digital cytometry of a biological sample, digital analysis of a biological sample, digital purification of a biological sample, evaluation of a disease in an individual, and prediction of a clinical outcome of a disease therapy.

The invention discloses an integrated cell mixing, sorting and electroporation micro-fluidic chip and a preparation method thereof. The chip is formed by bonding a PDMS (Polydimethylsiloxane) micro-channel layer and a glass substrate layer. The micro-channel layer is sequentially integrated with a Tesla mixer, a contraction-expansion array sorting unit and two independent electroporation chambers; the Tesla mixer is used for efficiently mixing fluids from the cell suspension inlet and the reagent inlet; the mixed cells are sorted into different flow channels according to the size based on the inertial effect in the sorting unit; the substrate layer is provided with two pairs of parallel plate copper electrodes with the interval of 200 microns, the positions of the parallel plate copper electrodes are accurately aligned with the two electroporation chambers respectively, and the parallel plate copper electrodes are used for applying electric pulses, so that differentiated efficient electroporation of cells with different sizes is realized. The three functions of uniform mixing, sorting and electroporation are organically integrated, automation, continuity and sealing of the whole cell treatment process are achieved, the treatment efficiency and consistency are remarkably improved, and the cell survival rate is remarkably increased.