40 results about "Mixed cell" patented technology

The present disclosure provides systems and methods for analyzing a mixed population of cells. In particular, the present disclosure provides systems and methods for digital cytometry of a biological sample, digital analysis of a biological sample, digital purification of a biological sample, evaluation of a disease in an individual, and prediction of a clinical outcome of a disease therapy.

The disclosure relates to growing cells, directing cells to grow into specified cell types, genetically and physically manipulating cells, and addressing one or more individual cells within a mixed cell population. Aspects of the disclosure relate to vectors useful to induce developmental changes in cells, in which those vectors have a temporal component. Vectors of the disclosure encode a controllable, temporal series of events. Once the vectors are delivered into target cells, a series of discrete and different genetic events may be induced. The disclosed methods generally provide for the temporal encoding of multiplex genetic effectors in vector format for cell state transitions.

The application discloses a cell liquid subpackaging method, which is applied to a cell liquid subpackaging system. The cell liquid subpackaging system comprises a peristaltic pump, a subpackaging bag, a calibration container and a diaphragm pump which are sequentially connected in the extension direction of the same subpackaging pipeline. The subpackaging method pumps cell liquid into the subpackaging bag first, then discharges the mixed cell liquid and the residual gas in the subpackaging bag into the subpackaging pipeline through the backflow mode, discharges the residual gas mixed in the subpackaging pipeline through the calibration container and the diaphragm pump on the subpackaging pipeline, obtains the cell liquid after gas-liquid separation, and then pumps the cell liquid after gas-liquid separation into the subpackaging bag again, so that the subpackaging product without residual bubbles is obtained, and the subpackaging quality is improved.

The invention relates to an electrochemical cell unit comprising the following, in the following order across its thickness: a hydrogen electrode (200) comprising a support electrode layer (203) and a functional electrode layer (204) stacked one on top of the other; a solid electrolyte (300) comprising a thin layer (302) having a first surface (305) and a second surface (306); and an oxygen electrode (400), characterised in that the solid electrolyte (300) comprises a frame (303) which extends from the first face (305) of the thin layer (302) and forms a cavity (304) in which the hydrogen electrode (200) is at least partially arranged. The invention relates to the optimisation of a solid oxide cell, including solid oxide fuel cells and solid oxide electrolyser cells.

Mixed cell unit with supporting electrode and electrolyte and methods of manufacturing same The invention relates to an electrochemical cell unit comprising in the order according to its thickness: a hydrogen electrode (200) comprising a supporting electrode layer (203) and a functional electrode layer (204) stacked on top of each other, a solid electrolyte (300) comprising a thin layer (302) having a first surface (305) and a second surface (306), an oxygen electrode (400) characterized in that the solid electrolyte (300) comprises a frame (303) extending from the first face (305) of the thin layer (302) and forming a cavity (304) in which the hydrogen electrode (200) is arranged at least in part. The invention relates to the optimization of a solid oxide cell including solid oxide fuel cells or cells and solid oxide electrolyzer cells. Figure for abstract: Fig.1

The invention discloses a multilayer adherent cell culture reactor and a use method. The culture reactor comprises a shell, a plurality of layers of cell culture plates are arranged in the shell in an array manner; and the liquid inlet is used as an inlet of a cell suspension. The liquid outlet is an outlet of the culture medium; the air holes are formed in the upper surface and the lower surface of the shell. And the quality control chamber is communicated with the inner cavity of the shell. The use method comprises the following steps: uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for positive culture; after front culture is completed, the multi-layer adherent cell culture device is inverted; uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for reverse culture. By arranging the multiple layers of cell culture plates with double surfaces capable of culturing, the cell culture area is greatly increased, the space utilization rate is improved, the use amount of a culture medium is reduced, the prepared quality control chamber is communicated with the inner cavity of the shell, and the cell growth state is conveniently, rapidly and accurately observed on the premise of not polluting the internal environment and not damaging the culture stability through isolation of the partition opening.

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

The application provides a method for preparing FFPE quality control products by using cell lines, a reference product and a kit, and belongs to the fields of medicine, clinical laboratory science and biotechnology, and comprises the following steps: culturing cell lines by using microcarriers; mixing different cell lines based on requirements; centrifuging the mixed cell microcarrier suspension in a sealed malt pipe at one end; removing supernatant after the treatment; packaging the two ends of the malt pipe by using a semi-permeable membrane; removing the semi-permeable membrane after fixing, dehydrating and transparentizing the malt pipe by using a formaldehyde solution; separating the cell microcarriers after the wax immersion treatment from the malt pipe; and embedding the cell microcarriers into a paraffin block by using wax. The quality control product can be used in subsequent quality control processes, the mutation frequency of the quality control product can be kept in good uniformity, and batch production and large-scale production of the quality control product can be realized.

The present invention relates to a cell population of spiral ganglion neural progenitors (SGNPs) and methods how to isolate these cells from an in-vitro mixed cell population. The cell population expresses one or more markers, wherein the one or more marker is CXCR4, SUSD2, LPAR3, EPHA5, CHRNA3, REEP1, NPFFR2, SORCS3, NFASC, GABRB3, KCNB2, GRM8, DLL3, and / or KCNH8.

The invention discloses a method for efficiently and directionally inducing IPS to be differentiated into pancreatic beta cells. According to the method, through staged signal regulation and strict cell screening, the directionality and the overall efficiency of differentiation are remarkably improved. The whole process pays attention to cell quality control from the initial stage, and a stable foundation is laid for subsequent differentiation by screening iPS cells with uniform morphology and high expression of pluripotent markers. Progressive signal molecule regulation is adopted in each stage, and an accurate differentiation path is formed from Activin A gradient adjustment during endoderm induction, synergistic effect of FGF10 and retinoic acid in pancreatic precursor cell differentiation and Wnt signal inhibition during endocrine precursor cell induction, so that the non-specific differentiation tendency is effectively reduced. Meanwhile, cell enrichment steps of key nodes, such as SOX17 positive cell screening in an endoderm stage and Ngn3 magnetic bead sorting of endocrine precursor cells, further improve the purity of a target cell population and avoid interference of parenchyma cells on subsequent function maturation.

The invention provides a microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device, and relates to the technical field of microalgae cell screening and detection. The microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device comprises a signal processing unit, the substrate is ITO (Indium Tin Oxide) conductive glass; the main micro-channel is of a micro-channel structure which is formed in a polydimethylsiloxane (PDMS) layer and is bonded on the substrate. According to the invention, the passive sorting module based on the expansion and contraction inertia effect and the differential impedance detection module are integrated in the single micro-fluidic chip, so that the full-process automation of'sample introduction-sorting-detection-output 'is realized. Sample loss, cross contamination and activity damage are avoided, interference of mixed cell populations on subsequent impedance signals is effectively eliminated through pre-sorting, and the accuracy of target microalgae detection is remarkably improved.

The present disclosure provides a method of performing a functional assay on human spheroids, e.g., three-dimensional human cell spheroids using, in one embodiment, a fluorometric imaging plate reader.

The invention discloses a cell multi-modal analysis system and method based on a micro-fluidic chip, and the system comprises a data collection and processing module which is used for inputting a mixed cell sample, a first cell sample screened by a cell classification module and a second cell sample output by a cell three-dimensional reconstruction module into the micro-fluidic chip, respectively acquiring a first bright field image, a second bright field image and a third bright field image; the cell classification module is used for receiving the first bright field image, outputting a cell category probability value through a cell classification model, screening a first cell sample and feeding back the first cell sample to the data acquisition and processing module; the cell three-dimensional reconstruction module is used for receiving the second bright field image, outputting a second cell sample through a cell reconstruction model, and feeding back the second cell sample to the data acquisition and processing module; and the cell deformation analysis module is used for receiving the third bright field image and outputting deformation data through a cell deformation model. According to the invention, cell multi-modal analysis can be accurately and quickly carried out at low cost.

The application discloses a cell multi-modal analysis system and method based on a micro-fluidic chip, and the system comprises a data acquisition and processing module, a cell classification module and a cell three-dimensional reconstruction module.

The invention relates to the technical field of cell culture, in particular to a 3D culture and purification method for olfactory ensheathing cells and olfactory ensheathing cell spheres. The method comprises the following steps: carrying out isolated culture on a mixed solution containing primary olfactory ensheathing cells to obtain mixed cell spheres; incubating the mixed cell spheres on a low-adsorption culture plate to obtain purified olfactory ensheathing cell spheres; the surface of the low-adsorption culture plate is a hydrophobic surface. The method can avoid the use of unstable cell markers, and effectively realizes the separation and purification of olfactory ensheathing cells; the purified olfactory ensheathing cells have good biological activity, and research and medical application related to the olfactory ensheathing cells can be more effectively promoted.

The utility model relates to the technical field of sample collection tools, in particular to a cell sap sample collection bottle, which comprises a mounting tube, and further comprises a micro valve which is vertically and fixedly arranged in the mounting tube and is used for controlling the flow of cell sap; the collecting bottle is fixedly arranged at one end of the mounting pipe, is communicated with the micro valve and is used for collecting the mixed cell sap; the reaction bottle is fixedly arranged at the other end of the mounting pipe, and the reaction bottle is communicated with the collecting bottle through a micro valve. In the embodiment of the utility model, the reaction bottle is connected with the collection bottle through the installation pipe, and the micro valve installed in the installation pipe can communicate the reaction bottle with the installation pipe, so that after cell sap in the reaction bottle is mixed with a sample swab, the mixed sample cell sap can be discharged into the collection bottle for collection through the micro valve; therefore, when the sampling swab in the reaction bottle is taken out, the cell sap cannot be polluted, and the accuracy of sample detection data is improved.

The disclosure relates to growing cells, directing cells to grow into specified cell types, genetically and physically manipulating cells, and addressing one or more individual cells within a mixed cell population. Aspects of the disclosure relate to vectors useful to induce developmental changes in cells, in which those vectors have a temporal component. Vectors of the disclosure encode a controllable, temporal series of events. Once the vectors are delivered into target cells, a series of discrete and different genetic events may be induced. The disclosed methods generally provide for the temporal encoding of multiplex genetic effectors in vector format for cell state transitions.

An energy efficient aquaculture system combining mixed-cell and raceway configurations. The system comprises a raceway tank, a raceway channel, a first water purification subsystem, and a second water purification subsystem. The system may include one or more of a hatching subsystem, a nursery subsystem, a feeding subsystem, a finishing subsystem, and a fish pumping system for transfer of fish between raceway tanks. A method of growing fish for commercial production using the aquaculture system is also provided.

The invention provides a method for preparing an FFPE quality control product by using a cell line, a reference product and a kit, and belongs to the technical field of medicine, clinical laboratory science and biologication.The method comprises the steps that the cell line is cultured by adopting a microcarrier; mixing different cell lines based on requirements; pouring the mixed cell microcarrier suspension into a straw with one sealed end, carrying out centrifugal treatment, removing the supernatant after the treatment is finished, then packaging the two ends of the straw by adopting semi-permeable membranes, carrying out formaldehyde solution fixation, dehydration and transparent treatment, removing the semi-permeable membranes, and carrying out wax dipping treatment; and separating the cell microcarrier subjected to wax dipping treatment from the straw, and then embedding the cell microcarrier with wax to form a paraffin block. The mutation frequency of the quality control product can keep good uniformity in the subsequent quality control process, and batch and large-scale production of the quality control product is achieved.

The embodiment of the invention provides a single cell sorting device. The single cell sorting device comprises a distributor, a mixer and an on-demand titrator, the distributor comprises a sample container and a leading-out device, a containing cavity is formed in the sample container, at least part of the cavity wall of the containing cavity is arranged to be a deformable part, the leading-out device is arranged below the sample container and connected to a bottom outlet of the containing cavity, the mixer is arranged relative to the sample container, and the on-demand titrator is arranged relative to the leading-out device; the use method of the single cell sorting device comprises the following steps: S1, adding a cell solution sample into the accommodating cavity from the input port of the accommodating cavity; and S2, starting the mixer and the on-demand titrator, applying an external force to the deformable part by the mixer so as to uniformly mix the cell solution in the accommodating cavity, enabling the uniformly mixed cell solution to enter the exporting device, and applying an external force to the exporting device by the on-demand titrator so as to export the cell solution from the exporting device in a liquid drop form. According to the embodiment of the invention, the technical effects of relatively high automation degree and prevention of pollution caused by manual intervention are achieved.

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

The invention relates to an NGS method for detecting the proportion of donors in a recipient. The method comprises the following steps: obtaining genotype mixed cell samples of the donors and the recipient; the genotype mixed cell sample is sequenced to obtain a homozygous gene combination and a heterozygous gene combination, so that the detection data can be reduced, and the detection cost is reduced; furthermore, the homozygous gene combination and the heterozygous gene combination can be counted to obtain a plurality of SNP sites, a plurality of InDel sites and a probability density map; respectively analyzing a plurality of SNP loci and a plurality of InDel loci in the homozygous gene combination and the heterozygous gene combination according to the probability density map to obtain an NGS result which comprises the ddcfDNA content of the donor and quantitative information of each InDel locus and is used for detecting the proportion of the donor in the recipient; therefore, SNP site and InDel site analysis can be carried out on a donor and a recipient, so that the obtained donor DNA content information is more accurate.

Materials and methods for labeling and isolating particular cell types from mixed cell populations are provided herein. Also provided herein are methods for generating data representing a synthetic genetic sequence configured for labeling at least one cell type by causing expression of a marker in the at least one cell type.

Semiconductor structures are provided. A semiconductor structure includes a cell array. The cell array includes a first regular cell, a second regular cell and a first mixed cell. Each P-type transistor has a first threshold voltage and each N-type transistor has a second threshold voltage in the first regular cell. Each P-type transistor has a third threshold voltage and each N-type transistor has a fourth threshold voltage in the second regular cell. Each P-type transistor has the first threshold voltage and each N-type transistor has the fourth threshold voltage in the first mixed cell. The first regular cell, the second regular cell and the first mixed cell are arranged in the same row of the cell array. The first mixed cell is arranged between the first and second regular cells and is in contact with the first regular cell.

Provided herein are methods of enriching for photoreceptors and photoreceptor precursor cells from mixed cell populations. Such enriched populations of photoreceptors and photoreceptor precursor cells can be used as cell therapies for the treatment of retinal disorders.

The present disclosure provides systems and methods for analyzing a mixed population of cells. In particular, the present disclosure provides systems and methods for digital cytometry of a biological sample, digital analysis of a biological sample, digital purification of a biological sample, evaluation of a disease in an individual, and prediction of a clinical outcome of a disease therapy.

Methods and associated reagents for assessing and resolving nucleic acid mixtures and / or mixed cell populations are disclosed herein. Some embodiments of the technology are directed to utilizing Duplex Sequencing for assessing and resolving nucleic acid mixtures (e.g., multichimeric mixtures, mixtures of nucleic acids from more than one source, etc.) in a sample and associated applications. Other embodiments are directed to detecting and quantifying a donor source of nucleic acid from a mixture.

The invention relates to the technical field of biomedical treatment, and discloses a magnetic bead incubation method which comprises the following steps: carrying out cell sorting and concentration on a cell sample in a centrifugal container to obtain concentrated cell sap; adding an incubation solution into the centrifugal container containing the concentrated cell sap, mixing, and performing magnetic bead incubation on the obtained mixed cell sap to obtain a target cell sap; and sub-packaging the target cell sap into a sub-packaging container. Separation of a large number of cell samples and concentration of the cell samples are achieved, non-specific binding of magnetic bead incubation is reduced by adding incubation liquid, the effect of magnetic bead incubation is improved, then the efficiency of magnetic bead incubation is improved, and meanwhile the pollution risk in the magnetic bead incubation process is greatly reduced.

The invention provides a construction method of a pleural effusion-derived tumor cell line of a lung adenocarcinoma patient, and belongs to the technical field of cell construction. The method comprises the following steps: carrying out single cell level accurate separation and purification on pleural effusion of a lung adenocarcinoma patient, carrying out cell type identification and tumor cell pre-enrichment in combination with a single cell transcriptome sequencing technology before primary culture, and carrying out incubation, subculture and subsequent accurate enrichment. And a tumor cell line which is highly enriched and has stable genetic characteristics is successfully obtained. The tumor cell line constructed by the method disclosed by the invention can be passaged for multiple times, so that individualized tumor antigen information and unique gene expression profiles of patients are reserved to the greatest extent, and meanwhile, the challenges of serious hybrid cell pollution, low tumor cell purity, low culture success rate and the like in the traditional method are overcome.