747results about How to "Efficient killing" patented technology

The invention discloses multifunctional hydrogel and application thereof, and belongs to the technical field of biological medicine. According to the multifunctional hydrogel, ketal mercaptan with two hydrazide ends and oxidized dextran are cross-linked to form a cross-linked network containing dynamic covalent bonds. The preparation method comprises the following steps: dissolving ketal mercaptan with two hydrazide ends in deionized water to obtain a ketal mercaptan solution, dissolving oxidized dextran in deionized water to obtain an oxidized dextran solution, mixing the ketal mercaptan solution with the oxidized dextran solution, and standing to obtain the multifunctional hydrogel. The multifunctional hydrogel has pH / ROS dual responsiveness, can respond to inflammation microenvironment and low pH of infectious wounds, remove active oxygen, release therapeutic drugs, regulate macrophage polarization and relieve bacterial infection, thereby playing a key role in inflammation, proliferation and remodeling stages of wound healing to accelerate wound healing. The curcumin-encapsulated liposome is added into the multifunctional hydrogel, so that the antibacterial effect can be further improved.

The invention discloses a neuraminidase-targeted drug-loaded nanoparticle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. Polysialic acid and poly (lactic-co-glycolic acid) are connected through ester bond connection to obtain the drug-loaded nanoparticles with stable structure and performance, and the drug-loaded nanoparticles have the effect of targeting neuraminidase and also have high efficiency of entering cells; the antibiotic drug is further dispersed in the drug-loaded nanoparticles, the inflammation regulating agent is adsorbed on the surface of the drug-loaded nanoparticles, and the drug-loaded nanoparticles can deliver the antibiotic drug and the inflammation regulating agent to the pig Gleisseria in the pig body. According to the present invention, the antibiotic drug and the inflammation regulating agent are released under the acidic environment at the infection site in the pig body, such that the pig Gleisseria is efficiently killed, the inflammation damage caused by bacterial infection and the toxic damage of the antibiotic drug are reduced, and the good application prospect is provided in the preparation of the drug for preventing and / or treating the pig Gleisseria infection.

The invention discloses a MAGE-A10 specific TCR (T cell receptor) and an application thereof. The invention provides a TCR (T cell receptor) which has high antigen sensitivity and can remarkably kill target cells, and also provides nucleic acid for coding the TCR, a carrier comprising the nucleic acid, a cell, a pharmaceutical composition and application of the TCR in preparation of the pharmaceutical composition for treating tumors and screening of antigen detection products.

The invention discloses a GPC3 derived antigen peptide vaccine, an mRNA vaccine, a saRNA vaccine and a preparation method and application thereof. The amino acid sequence of the antigen peptide vaccine is one or any combination of more of amino acid sequences shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. According to the invention, an efficient pGPC3 model antigen peptide is screened on the basis of GPC protein, and pGPC3 mRNA and pGPC3 saRNA are designed according to a pGPC3 sequence example. The pGPC3saRNA (at) Lipi vaccine is prepared through a liposome technology, and due to the fact that limiting conditions such as nonapeptide and high-affinity binding with H-2I type molecules (H-2Kb) are adopted during pGPC3 polypeptide antigen screening, APC cells are facilitated to present antigens through an MHCI restrictive pathway, CD8 + T cells are activated, and GPC3 specific CTL is triggered to efficiently kill liver cancer cells. Finally, the pGPC3saRNA (at) Lipi vaccine can completely inhibit the growth of the liver cancer, the inhibition rate reaches 99.8%, and the liver cancer of 4 / 8 mice completely fades down. In addition, the method can be used for vaccine adjuvant and carrier research on a liver cancer subcutaneous transplantation tumor model.

The invention provides a fiber dressing for promoting wound healing and a preparation method and application thereof, and belongs to the technical field of biological materials. The shell of the fiber dressing contains the first medicine for promoting polarization of macrophages, and the first medicine can promote polarization of macrophages at normal and diabetic wound surfaces from M1 type to M2 type; a shell substrate is a mixture of hydrophilic and hydrophobic polymers, so that painless adhesion and stripping of a wound surface can be realized by absorbing seepage and spraying non-irritant normal saline on the wound surface, and secondary injury of the wound surface is reduced; an inner core contains a second medicine and a third medicine which are used for inducing death of M1 type macrophages, so that wound inflammatory response can be reduced, wound bacteria can be killed, wound infection degree can be reduced, and wound healing can be accelerated; the core matrix is a mixture of hydrophilic and hydrophobic polymers with good biocompatibility, can provide mechanical support for the fiber dressing, and provides a slow release effect for the two substances in the core. By controlling the composition of the inner core and the shell, the healing of normal wounds and diabetic wounds can be effectively promoted.

The invention relates to the technical field of farm disinfection, in particular to an intelligent disinfection system for a farm. The system comprises an initial determination module, a periodic disinfection module, an environment monitoring module, a first analysis control module, a second analysis control module and a feedback correction module. According to the method, the initial disinfection parameter cluster of each breeding area in the target breeding farm is determined according to the actual growth stages of the breeding targets and the actual number corresponding to each actual growth stage, each breeding area is periodically disinfected according to the initial disinfection parameter cluster, and each breeding area is monitored in real time so as to be singly judged; and performing first-class adjustment and second-class adjustment on the initial disinfection parameter cluster based on a first verification result and a second verification result in combination with the first verification result and the second verification result to determine whether the initial disinfection parameter cluster is subjected to first-class adjustment and second-class adjustment. And according to the first analysis control result and the second analysis control result, determining whether to perform feedback correction on a preset initial disinfection period of each breeding area.

The invention provides a bionic antibacterial peptide, a pharmaceutical composition, application of the bionic antibacterial peptide in preparation of antibacterial drugs and a design method of the bionic antibacterial peptide. A plurality of compounds are used for carrying out N-terminal modification on a polypeptide sequence consisting of 20 natural amino acids, so that a novel antibacterial peptide with a bionic function is generated, and the designed bionic antibacterial peptide is screened by adopting a method based on deep learning and experimental interaction. Compared with traditional drug screening, consumed time, manpower, material resources and financial resources are greatly reduced, the designed novel bionic antibacterial peptide has good biocompatibility, a functional group modified at the N end of the novel bionic antibacterial peptide can activate the self-assembly activity of polypeptide and help the polypeptide target a bacterial membrane, bacterial drug resistance is not likely to be caused, and the novel bionic antibacterial peptide has good application prospects. The drug-resistant bacteria can be effectively killed, the sterilization speed is higher, and potential and wide application prospects are achieved in clinical treatment of bacteria and drug-resistant bacteria infection diseases.

The invention relates to the technical field of bactericides, and discloses an oilfield flooding bactericide containing plant bactericidal components and a preparation method thereof. The preparation method comprises the following steps: loading the plant-based traditional Chinese medicine extract and the isothiazolinone-based corrosion inhibitor on the silver-loaded cyclodextrin metal organic framework to prepare an antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material, and mixing the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material with methyl methacrylate to prepare the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material. The bactericide obtained by carrying out copolymerization reaction on modified hydroxyquinoline, vinyl quaternary phosphonium salt and methacrylic acid through an in-situ polymerization method can effectively kill and inhibit the breeding of sulfate reducing bacteria and other bacteria in oilfield reinjection water, and has a certain corrosion inhibition effect.

The invention provides thiamethoxam water dispersible granules and a production process thereof, and belongs to the technical field of pesticide preparation. The production process comprises the following steps: modifying methyl methacrylate; preparing an anti-ultraviolet carrier; loading eugenol; and preparing the water dispersible granules. The water dispersible granule is prepared by mixing the uvioresistant carrier loaded with eugenol with thiamethoxam, the dispersing agent, the wetting agent, the disintegrating agent and the binder, when eugenol and thiamethoxam are combined for use, the pest killing effect can be synergistically enhanced, and in addition, eugenol can bypass the resistance mechanism of pests to thiamethoxam, so that the pest killing effect is improved. Even if pests have certain tolerance to neurotoxicity of thiamethoxam, eugenol can still play a role by destroying cell membrane structures and respiratory systems of the pests, so that the resistance of the pests is broken, and the overall insecticidal effect of the prepared water dispersible granule is enhanced.

The invention provides a high-salinity high-tidal-flat mangrove forest ecological restoration method. The high-salinity high-tidal-flat mangrove forest ecological restoration method comprises the following steps of S1, soil improvement, S2, seedling cultivation, S3, seedling planting, S4, seedling reseeding, S5, bank protection device arrangement and S6, pest control. The soil conditioner is used for improving the soil structure and reducing the soil salinity. Salt-resistant domestication is performed on the seedlings by gradually improving the salinity of irrigation water, so that the salt resistance of the seedlings is improved. Organic fertilizer, ferriporphyrin, abscisic acid and brassinolide are used for providing nutrients for seedlings, osmotic equilibrium in plants is adjusted, opening and closing of air holes are adjusted, plasticity of cell walls is adjusted, and therefore the salt resistance of the plants is enhanced. According to the method, the roots of seedlings are inoculated with the halorhodobryum terbach and the proteobacteria, the halorhodobryum terbach and the proteobacteria terbach form a symbiotic relationship with the roots of plants, a microbial community is formed, and the absorption efficiency of the plants to nutrients in soil is According to the method, the planting survival rate and the plant growth rate are improved through the multi-way overall synergistic effect, and then the biological diversity is improved.

The utility model relates to a livestock farm ventilation disinfection device which comprises a ventilation structure shell and a disinfection assembly located in the ventilation structure shell, the disinfection assembly comprises a primary filter screen, a micro-static air purification module and an ultraviolet disinfection module which are sequentially arranged in the air flowing direction, and a fan is installed at one end of the ventilation structure shell. The fan is located on the side, away from the micro-electrostatic air purification module, of the ultraviolet disinfection module. Under the power action of the fan, air with bacteria is primarily filtered through the primary filter screen, then is dedusted and purified through the micro-electrostatic air purification module and then is subjected to ultraviolet deactivation through the ultraviolet disinfection module, ventilation is still kept in the feeding period, meanwhile, through front-stage filtering and rear-stage disinfection and sterilization, the air quality is improved, and the breeding efficiency is improved. The disinfection efficiency and effect are improved, so that the concentration of bacteria in the air is reduced, and the infection probability is reduced.

The invention provides hydrogel wound dressing and a preparation method thereof.The hydrogel wound dressing comprises a hydrogel layer, a supporting layer and a protective layer, the hydrogel layer is prepared from, by mass, 10%-15% of polyvinylpyrrolidone, 5%-8% of polycaprolactone, 2%-4% of sodium carboxymethyl cellulose, 1%-3% of chitosan, 0.5%-1.5% of an antibacterial agent, 0.1%-0.3% of an antioxidant and the balance water; the invention discloses a graphene quantum dot gel which comprises the following components in percentage by weight: 0.01-0.1% of a growth factor, 0.1-0.5% of a temperature-sensitive polymer, 0.05-0.15% of graphene quantum dots, 0.5-1.5% of a self-healing polymer, 0.5-1.5% of a pH sensitive material, 0.1-0.3% of nano titanium dioxide and the balance of deionized water. The supporting layer is a porous polylactic acid film, and the thickness of the supporting layer is 0.1-0.3 mm; the protective layer is an air-permeable polyurethane film, and the thickness of the protective layer is 0.2-0.4 mm. Through the synergistic effect of an antibacterial agent, nano titanium dioxide and graphene quantum dots, the antibacterial performance of the dressing is remarkably improved.

The invention provides a culture method for improving immunity of fishes. The culture method comprises the following steps: S1, selection of culture points; S2, specification and layout of net cages; S3, selection and throwing of fish fries; according to the fry disinfection method disclosed by the invention, the fry are soaked and disinfected by using a compound disinfectant prepared from povidone iodine, polysorbate, citric acid and vitamin C in a specific proportion, so that pathogenic microorganisms on the surfaces and gill parts of fish bodies are effectively killed. According to the breeding management, the immunity of the fish body is comprehensively enhanced by feeding the fish with an immune conditioner composed of campesterol palmitate, immunomodulatory peptide, beta-glucan and astaxanthin in a specific proportion. According to the bottom mud management, the content of harmful substances is reduced by putting a bottom mud modifier composed of sodium molybdate, nitrifying bacteria, photosynthetic bacteria, tetrandrine and sodium alginate in a specific proportion, growth of pathogenic bacteria is inhibited, nutrient substance circulation is promoted, the water quality environment is optimized, and healthy growth of fry is promoted.

The invention provides an intercropping method of tobacco and waltheria indica. The intercropping method comprises the following steps that 1, before tobacco and waltheria indica are planted, soil is deeply ploughed, a composite agent is sprayed, and then base fertilizer is applied; the composite agent is prepared from the following raw materials: ammonium sulfate, tea tree oil, rhizobium and a peanut vine extract; (2) tobacco seedlings are selected and transplanted in February to March, waltheria indica is planted between rows, and after planting is conducted for 2-3 weeks, compound fertilizer is applied between the rows of the tobacco seedlings and the waltheria indica; (3) when rainstorm weather occurs, spraying a complex microbial inoculant within 24 hours after rainstorm, and (4) managing until harvesting tobacco and waltheria indica. By the adoption of the intercropping method, nematodes in soil are inhibited, the tobacco and the waltheria indica are effectively improved, symbiosis and growth of the tobacco and the waltheria indica are promoted, and the harvesting yield per mu of the tobacco and the waltheria indica is increased.

The invention discloses a method for converting livestock and poultry manure into an organic fertilizer through microbial fermentation, and belongs to the technical field of livestock and poultry manure conversion. The method comprises the steps that firstly, the livestock and poultry manure is subjected to physical sorting, lime or monopotassium phosphate is added according to the pH value detection result, and the pH value is adjusted to 6.5-7.5; after determining nutrient components of the feces, adding soluble salt trace elements such as zinc sulfate and mixed carbohydrates of cane sugar and glucose; traditional strains of bacillus, lactic acid bacteria, saccharomycetes and actinomycetes are mixed with novel strains separated from the periphery of a livestock and poultry farm, optimized culture is conducted through a two-step culture method, and plant extracts such as lily extracts or livestock and poultry intestinal metabolites such as short-chain fatty acid are added in the culture process. The fermentation process adopts a rotatable fermentation tank body, and comprises three stages of fermentation of rapid heating, high-temperature stabilization and cooling decomposition. And finally, low-temperature vacuum drying, crushing, granulation and addition of a coating material are performed to prepare the organic fertilizer, so that efficient resource utilization of the livestock and poultry manure is realized.

The invention provides a ZIF-8-based composite antibacterial material and a preparation method and application thereof.Ce6 is efficiently packaged into ZIF-8 through a simple one-pot method, Ce6 at-ZIF-8 is synthesized, Ce6 at-ZIF-8 is endowed with excellent photodynamic bacterium killing efficiency, then MnO2 is doped into Ce6 at-ZIF-8, the antibacterial efficiency is greatly improved under photodynamic and chemical kinetics synergistic antibacterial effects, and meanwhile, the composite antibacterial material can be used for preparing a composite antibacterial material. Due to the addition of berberine, the antibacterial effect is improved, the healing rate of a wound is increased, the berberine has good dispersity, can be uniformly dispersed in water, has large specific surface area, can well inhibit growth and adhesion of bacteria, can be used as a dressing for promoting wound healing or resisting bacteria, not only can effectively kill bacteria except the wound, but also can be used as a dressing for promoting wound healing or resisting bacteria. In particular, wound healing of diabetic patients can be promoted, and the antibacterial material can be used as a novel antibacterial material for antibiosis and wound healing.

The invention provides a preparation method of a PSR.Syn.CD19. CD38 CAR-T cell as well as the cell and application of the PSR.Syn.CD19. CD38 CAR-T cell, the PSR.Syn.CD19. CD38 CAR-T cell is characterized in that after the CAR-T cell and a CD19 antigen positive tumor cell are co-incubated, effector molecule anti-CD38-CAR can be induced to express, and when the tumor cell simultaneously expresses CD19 and CD38 antigens, the CAR-T cell capable of recognizing and killing tumors can be identified. The CAR-T cell provided by the invention can efficiently and specifically kill tumor cells (CD19 + CD38 +), has a persistent killing effect, and has a safety switch capable of starting a killing function. Besides, the tumor can be specifically killed, the cell proliferation rate is high, the anti-tumor function can be improved through a large amount of secreted IL-2, IFN-gamma, TNF-alpha and Granzyme B, and the application safety of the cell is greatly improved.

The invention discloses a monitoring and killing system and method applied to dam termite prevention and control, and relates to the technical field of dam termite prevention and control, and the system comprises a bionic termite nest monitoring device, a portable termite killing device, and a remote monitoring platform. The bionic ant nest monitoring device comprises a plurality of bionic ant nests, a bait casting opening is formed above the bionic ant nests, a telescopic bait casting rod is arranged in the bait casting opening, a bait placing platform and a data acquisition module are arranged in the bait casting opening, and a bait barrel is placed on the bait placing platform; the portable termite killing device is communicated with the bionic termite nest, termite killing gas is put into the bionic termite nest, the termite killing gas is diffused to the termite native channel through the bionic channel, and local termite killing is carried out; the remote monitoring platform processes and displays the monitoring data, and obtains the distribution condition of the native termite channels by analyzing the change trend of environmental conditions, thereby controlling the opening and closing of the portable termite killing device and the input amount of termite killing gas, and achieving the purpose of integrating the monitoring, killing and prevention of dam termites.

The invention designs and synthesizes a tetraphenyl ethylene covalent molecular cage with pyridine functionalization, and an AIE supermolecule photosensitizer is obtained through coordination and the synergistic effect of a subject and an object. The TPE unit enhances the photophysical properties of the photosensitizer and endows the photosensitizer with the AIE characteristic; the molecular cage and tetraphenylporphyrin are combined through a host and a guest, so that self-aggregation of porphyrin in water is further inhibited, ROS generation is promoted, and cancer cells are effectively killed through photocatalysis NADH oxidation assistance.

The invention relates to the field of bacteriostatic cream, and particularly discloses skin bacteriostatic cream and a preparation method thereof. The skin antibacterial cream is prepared from the following raw materials in parts by weight: 4 to 6 parts of bran coat extract, 2 to 4 parts of chlorhexidine salt, 2 to 4 parts of rutin extract, 1 to 3 parts of carnosine, 0.5 to 0.8 part of sodium hyaluronate, 0.8 to 1.2 parts of film-forming agent and 5 to 8 parts of purified water. The preparation method comprises the following steps: mixing the film-forming agent and a part of water, and uniformly stirring to obtain a film-forming agent solution; the preparation method comprises the following steps: mixing sodium hyaluronate and water, heating, stirring until the sodium hyaluronate and the water are completely dissolved, adding the cavings extract, the rutin extract, the chlorhexidine salt and the carnosine, stirring until the mixture is uniformly dispersed, heating to 75-85 DEG C, emulsifying for 25-35 minutes, homogenizing, adding a film-forming agent solution into the homogenized emulsion, and stirring, concentrating, degassing and discharging the paste in vacuum to obtain the antibacterial paste. The composition can be used for diminishing inflammation and resisting bacteria of damaged skin, and has the advantages of being high in antibacterial rate and capable of avoiding skin pigmentation.

The invention discloses a polydopamine modified and cupric peroxide loaded ZIF8 nano particle and application thereof, and belongs to the technical field of medicine preparation. According to the invention, a zeolite imidazoline framework 8 is used as a matrix, CuO2NPs is uniformly modified in the zeolite imidazoline framework 8 to form CuO2 (at) ZIF8NPs, and the surface of the CuO2 (at) ZIF8NPs is coated with a polydopamine film in the form of a film. The invention constructs multifunctional ZIF8NPs (CuO2 (at) ZIF8 (at) PDANPs) which is coated with PDA and loaded with CuO2NPs, and the multifunctional ZIF8NPs (CuO2 (at) ZIF8 (at) PDANPs is used for CDT of thermal enhancement OS. The CuO2 (at) ZIF8 (at) PDA NPs is combined with a photothermal effect and a chemodynamic therapy, shows good biocompatibility and an anti-tumor curative effect, has a potential application prospect in the aspect of osteosarcoma, and provides a new thought and method for treatment of osteosarcoma.

The invention belongs to the technical field of bioengineering, and particularly discloses an in-vitro amplification method of canine NK cells capable of efficiently killing tumor cells and application of the canine NK cells. 3 mL of dog peripheral blood is taken, and dog peripheral blood mononuclear cells PBMC are obtained through separation; activating, amplifying and culturing canine NK cells capable of efficiently killing tumor cells; and collecting the amplified canine NK cells capable of efficiently killing the tumor cells, and storing the canine NK cells in a cryopreservation solution according to the cell density of 1 * 10 < 8 > cells / mL. According to the method, the canine NK cell with high amplification multiple can be obtained by only needing 3mL of canine peripheral blood, and the amplification multiple is high; the number of the dog NK cells of the healthy volunteer dog sample is 3.02 * 108, the amplification multiple is 1008, the total number of the dog NK cells of the tumor volunteer affected dog sample reaches 6.84 * 107, and the amplification multiple is 340; and the canine NK cell obtained by the invention has the effect of efficiently killing tumor cells.

The present invention discloses a strain of Riemerella anatipestifer phage PT15, a phage composition thereof and its application. The phage PT15 has a deposit number of CGMCC No. 22377 and was deposited in the China General Microbiological Culture Collection Center on April 12, 2021. The present application also provides a phage composition comprising the above-mentioned phage. The above-mentioned phage and phage composition can be used as active ingredients for the preparation of therapeutic drugs and feed additives, and are used in the prevention and treatment of duck bacterial serositis. The phage composition has the advantages of safety, high efficiency and non-toxic side effects, and can be used as an active ingredient of a new type of environmental disinfectant to effectively prevent and control the pollution of breeding environment and feeding facilities, and has good application prospects.

The invention discloses a herbicide composition for preventing and treating amaranthus retroflexus in alfalfa fields and application thereof, and belongs to the technical field of herbicides, the herbicide composition comprises a component A and a component B. The component A is imazethapyr / or 2, 4-sodium butyrate, and the component B is haloxyfop-R-methyl; the composition has good selectivity to alfalfa and can effectively prevent and control amaranthus retroflexus, the earlier the pesticide application period is, the better the control effect is, the damage to alfalfa plants is small, and normal growth of alfalfa is guaranteed. Due to the fact that the composition contains imazethapyr or 2, 4-sodium butyrate, and the ingredients have a certain residual period, the weeding effect can be continuously achieved in a long time, the pesticide application frequency is reduced, and the labor intensity and cost are reduced. The 2, 4-D sodium butyrate and the compound thereof have a better control effect on amaranthus retroflexus and are high in safety to medicago parasitica. The invention provides an effective and safe herbicide for controlling amaranthus retroflexus in alfalfa fields.

The invention provides a blue-green algae inhibitor accurate spraying method and system based on an unmanned ship, and belongs to the technical field of blue-green algae treatment. The technical problem that the dosage of the blue-green algae inhibitor cannot be accurately controlled in the blue-green algae killing process is solved. According to the technical scheme, the spraying method comprises the following steps of collecting target water area environment data and blue-green algae concentration data, drawing a target water area route planning map, dividing the target water area route planning map into a plurality of sub-areas, judging target sub-areas needing to be sprayed with inhibitors, planning a spraying path of an unmanned ship, and determining a spraying scheme of each target sub-area. And setting a spraying path of the unmanned ship and a spraying scheme of each target sub-area, and accurately spraying the blue-green algae inhibitor to the target water area. The system comprises an unmanned ship, a planning and drawing module, a route planning module and a spraying control module. According to the method, the blue-green algae inhibitor is automatically and accurately sprayed to the target water area through the unmanned ship, the spraying path is planned, and automatic treatment of blue-green algae is achieved.

The invention discloses a highly antibacterial fabric and a preparation process thereof, and relates to the technical field of antibacterial fabrics, the highly antibacterial fabric comprises a composite fabric layer, an antibacterial adhesion layer, a zinc coating, a cotton fabric base material and a nano-silver antibacterial layer; the composite fabric layer is prepared from a polycaprolactone spinning solution and a polylactic acid spinning solution through electrostatic spinning, the polycaprolactone spinning solution is prepared from a polycaprolactone solution and a composite heterojunction suspension through mixing, and a composite heterojunction in the composite heterojunction suspension is prepared from sodium molybdate dihydrate and zinc through a hydrothermal method; the antibacterial adhesion layer is prepared from menthol modified polyacrylate; the zinc coating is prepared from a zinc target material through magnetron sputtering; the nano-silver antibacterial layer is prepared from hinokitiol modified chitosan through in-situ reduction of silver nitrate.

The invention provides a public hot water system utilizing high-energy waves for sterilization and a sterilization method, and belongs to the technical field of hot water sterilization, and the public hot water system comprises a hot water tank, an external circulation sterilization system and an internal circulation sterilization system. The external circulation sterilization system comprises a hot water outlet pipe, a flow meter, a first high-energy wave sterilization device, a one-way valve, a hot water return pipe, a first circulating pump, a second high-energy wave sterilization device, a water inlet valve and a cold water inlet pipe, and the internal circulation sterilization system comprises a circulating hot water pipe, a second circulating pump and a third high-energy wave sterilization device. According to the invention, physiological structures of microorganisms such as bacteria, viruses and the like can be destroyed to kill or inhibit the microorganisms; the water quality is purified, bacteria and microorganisms are prevented from breeding in the system, it is ensured that the microorganisms in the public hot water system are effectively killed, and the biological and chemical safety in the public hot water system is guaranteed.

The invention relates to an HER2 (human epidermal growth factor receptor 2) targeting antibody coupling gold (III) porphyrin medicine as well as a synthesis method and tumor treatment application thereof. The antibody coupling gold (III) porphyrin drug targeting HER2 is formed by connecting an anti-HER-2 trastuzumab and an A3B type gold (III) porphyrin complex through a linker, and the linker comprises a maleimide joint connected with an antibody and a self-elimination joint connected with the A3B type gold (III) porphyrin complex. The A3B type gold (III) porphyrin complex with anti-tumor activity is combined with trastuzumab with a specific recognition function as a carrier, the antibody is utilized to accurately strike tumor cells, a medicine is delivered to a tumor environment, the tumor targeting property of the gold (III) porphyrin complex is improved, and accurate treatment is realized. Distribution of drugs in the body can be improved, accumulation of the drugs in non-tumor sites can be reduced, the dosage of metal complexes is reduced, and toxic and side effects are reduced; the efficient delivery of gold drugs to tumor tissues can also be realized, and the cancer treatment effect is improved.

The invention relates to the technical field of genetic engineering antibodies, in particular to a bispecific antibody combined with BCMA and CD3 as well as a preparation method and application of the bispecific antibody. The invention provides a bispecific antibody which can be combined with BCMA and CD3 at the same time, has a specific targeting effect and can efficiently excite oriented immune response. The bispecific antibody well retains the biological functions of an anti-CD3 antibody and an anti-BCMA antibody, realizes that one bispecific antibody molecule simultaneously has an excellent BCMA and CD3 targeted binding biological function, can build a bridge between tumor cells and immune effector cells, effectively activates the immune effector cells and oriented immune response, and has a good application prospect. The tumor cell killing effect of immune cells is remarkably enhanced, the target cell killing efficiency is high, the ADCC effect is reduced to the maximum degree, high safety is achieved, and good application prospects are achieved in tumor immunotherapy.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 hours by means of portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 hours to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano-atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.