267 results about "Digestive enzyme" patented technology

The invention relates to the technical field of pet health care, in particular to a probiotic composite additive for pet intestinal health and a preparation method of the probiotic composite additive. Comprising the following basic components in percentage by mass: 40% of lactobacillus complex, 10% of bifidobacterium complex, 10% of saccharomyces boulardii, 10% of prebiotic complex, 10% of natural digestive enzyme, 10% of functional plant extract, 10% of carrier and the like. 30% of a bifidobacterium complex group; 15% of saccharomyces boulardii; 8% of a prebiotic compound; 5% of natural digestive enzyme; and 2% of a functional plant extract. The multiple probiotics have a synergistic effect, the effect is better, the prebiotics promote proliferation of the probiotics and can enhance the effect of the additive, meanwhile, through drying treatment, the product is good in stability, long in shelf life and small in package design, the additive can be conveniently added to pet food, intestinal health of pets can be effectively improved, and the preparation method is simple and suitable for large-scale production.

The present disclosure relates to single cell protein (SCP) products obtained from Cupriavidus necator that has been engineered to produce digestive enzymes or otherwise enhanced. The biomass produced by the C. necator and the SCP products that include it contain digestive enzymes that are not naturally produced by C. necator.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses a lactobacillus plantarum MW 2406C, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number of the lactobacillus plantarum MW 2406C is CGMCC No. 33339. The inventor finds that the bacterial strain has better oxidation resistance, pathogenic bacterium inhibition and oxygen resistance, and can be used for effectively fermenting feed, pre-decomposing nutritional ingredients in the feed and improving the utilization efficiency of the feed; in addition, the strain can improve liver and intestine health and improve immunity by enhancing hepatopancreas antioxidant enzyme activity and intestinal digestive enzyme activity, improving intestinal barrier health, up-regulating antibacterial peptide level and reducing inflammatory factors. When the lactobacillus plantarum is used for preparing the fermented feed for the river crabs, the utilization efficiency of the compound feed by the river crabs is improved, the intestinal health of the river crabs is improved, and the disease resistance is improved.

The invention discloses nutrition-balanced, sugar-controllable, fat-reducing and staple food nutrition-improving medicated food and a preparation method thereof. The medicated food is prepared from the following raw material components: 5-10% of high-quality protein, 1-10% of high-quality fat-containing food raw materials, 0.8-15% of medicinal and edible traditional Chinese medicines, 5-15% of high-quality carbohydrates, 5-60% of dietary fibers, 2-10% of functional sugar and 1-2% of minerals. The formula food is directly cooked with rice to form rice and porridge or is directly mixed with flour to form cooked wheaten food, so that the contact degree of starch and digestive enzyme and the activity of glycosidase can be reduced, the starch hydrolysis rate can be reduced, and the generation of postprandial blood sugar can be delayed. The formula food is prepared from various functional food raw materials and medicinal and edible food materials, is safe and free of side effects, and can be taken for a long time. All the components supplement each other to achieve a synergistic effect, the glycemic index of a patient can be adjusted, diabetic complications of the patient are improved, and therefore disease rehabilitation of the diabetic patient is promoted.

The invention discloses a pet hair-removing food and a preparation method thereof, and relates to the technical field of pet supplies, the pet hair-removing food comprises the following specific components: cellulose components, fat lubricating components, probiotics and prebiotics, natural digestive enzymes, plant extracts, vitamins and minerals, and natural vegetable oil; the additives specifically comprise a low-density microcapsule controlled release lubricant, a bioactive intestinal coating compound enzyme, modified polymer hydrated fibers and a plant-derived antistatic component; the preparation is carried out based on the above. According to the pet hair-removing food and the preparation method thereof, a plurality of additives are introduced, and particularly, a low-density microcapsule technology is used, so that lubricating components can be slowly released in intestinal tracts of pets, a longer-time lubricating effect is realized, and smooth discharge of hair is remarkably improved. In addition, the added bioactive intestinal coating compound enzyme and plant source antistatic component further enhance the health condition and hair removal effect of the intestinal tract, and reduce the occurrence of hair ball accumulation.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The present invention provides a method for preparing exosomes derived from antler periosteal mesenchymal stem cells and their applications. After obtaining antler periosteal tissue, the tissue is placed in a DPBS culture dish containing antibiotics for further processing. The periosteal tissue is then minced and washed with DPBS until it is free of blood. The minced tissue is treated with digestive enzymes and incubated in a carbon dioxide (CO2) incubator to facilitate enzymatic digestion. Subsequently, alpha-MEM complete culture medium is added to further process the tissue. The digested tissue is filtered and centrifuged to isolate the primary periosteal cells, which are then counted and cultured in a suitable medium to obtain mesenchymal stem cells (MSCs) derived from antler periosteum. The cultured periosteal MSCs are further processed to isolate exosomes, which can be applied to promote the proliferation and repair of human fibroblasts, enhance skin quality, and stimulate hair growth.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention provides a bacillus subtilis strain and application thereof, the bacillus subtilis strain is named as bacillus subtilis Ya3.1, is preserved in China General Microbiological Culture Collection Center on July 7, 2025, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35019; the bacillus subtilis Ya3.1 is separated from intestinal tracts of healthy hybrid sturgeons, and can be used as a probiotic additive for aquaculture, especially for sturgeon culture. The bacillus subtilis Ya3.1 provided by the invention has high safety, strong stress resistance and efficient intestinal colonization ability, and can significantly improve the activity of sturgeon intestinal digestive enzymes, improve the growth performance of sturgeons, enhance the non-specific immunity of sturgeons, improve the intestinal flora structure and significantly improve the ability to resist aeromonas bacterial infection; and a special probiotic resource can be provided for sturgeon culture.

The invention belongs to the technical field of cell extraction, and particularly relates to a method for extracting cells by enzymolysis of vitreous bodies, which comprises the following steps: centrifuging a vitreous body sample at room temperature, discarding supernate, and adding digestive enzyme into precipitate; after adding digestive enzyme, digesting in a water bath for 30 minutes; and after the enzymolysis is finished, centrifuging the enzymatic hydrolysate at room temperature, discarding the supernate, and collecting the precipitate, namely the enzymolysis vitreous cells. According to the technical scheme, a simple and efficient vitreous body enzymolysis method is provided, and a large number of cells can be provided for follow-up research.

The invention belongs to the technical field of materials, discloses a preparation method and application of novel stomach and intestinal tract passing sustained-release tannic acid, and particularly discloses a stomach and intestinal tract passing enteric coating material which comprises a surfactant, sugar alcohol, hydrogenated grease, polyacrylic resin and a thickening agent. The stomach-passing enteric coating material does not use expensive commercially available enteric coating materials, but adopts common surfactants, sugar alcohol substances and other substances, so that the grease and the water can be better fused, and the grease can be better coated outside in the coating process. The stomach-passing enteric coating material can ensure that tannic acid is not damaged by gastric acid, reduces the influence on animal digestive enzymes, and can be slowly released in intestinal tracts to be absorbed and utilized.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

The invention relates to the technical field of extracts, in particular to macromolecular dried longan pulp heteropolysaccharide with a blood sugar reducing characteristic and a preparation method and application thereof. The preparation method comprises the following steps: drying the longan pulp, homogenizing the dried pulp, adding distilled water, performing hot water extraction, filtering through gauze, and separating the dried longan pulp heteropolysaccharide with the blood sugar reducing characteristic and the molecular weight of more than 100kDa from the dried longan pulp by combining processes such as dialysis, ultrafiltration interception and the like. The dry longan pulp polysaccharide is extracted by adopting a hot water extraction method, the dry longan pulp polysaccharide is separated by adopting an ultrafiltration interception system device, the main component DLP of the dry longan pulp polysaccharide can inhibit starch digestive enzymes, model cell insulin resistance can be improved, and human intestinal flora composition can be changed. The beverage has the functions of reducing blood sugar and regulating intestinal flora.

The invention discloses a preparation method and application of a bovine tongue mucous membrane organoid, and relates to the technical field of organoid separation and culture. The preparation method of the ox tongue mucosa organoid comprises the following steps: treating fetal ox tongue tip tissues with digestive enzymes Dispase I, Collagenase II and Collagenase IV, separating ox tongue mucosa, cutting into pieces, treating again with pancreatin, and sieving to remove tissues and impurities; and continuously culturing by adopting a three-dimensional culture technology-matrigel embedding method and an organoid culture medium to obtain the final ox tongue mucous membrane organoid. The ox tongue mucous membrane organoid prepared by the preparation method disclosed by the invention has normal cell proliferation, differentiation and metabolism functions, and can be stably passaged under an in-vitro culture condition; the method can be widely applied to various related scientific researches such as FMDV infection mechanism research, drug screening and vaccine evaluation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The application provides a preparation method and application of oat active peptides for promoting GLP-1 secretion, wherein the oat active peptides contain any one or more of peptide segments NDQRGEII, KQGDVIALPA, PFVQQQQ and PSEQYQPYPEQQEPFVQ. The preparation method of the oat active peptides comprises enzymolysis, chemical synthesis or genetic engineering. The oat active peptides can significantly promote the secretion of GLP-1 by intestinal endocrine cells, and have the advantages of safe and non-toxic side effects, tolerance to gastrointestinal digestive enzyme hydrolysis and easy absorption. The application has simple operation, low requirement for equipment, is suitable for industrial large-scale production, and can be used for the development of new drugs for controlling blood sugar or food for assisting in controlling blood sugar, drugs for controlling body weight or food for assisting in controlling body weight and the like.

The invention provides an alginate lyase mutant Amut8C as well as a coding gene and application thereof, belongs to the technical field of gene engineering and enzyme engineering, and can solve the problem that existing alginate lyase cannot meet the requirements of severe reaction conditions such as high temperature and acidity in industrial application. The amino acid sequence of the alginate lyase mutant Amut8C disclosed by the invention is as shown in SEQ ID NO. 1. According to the space structure of the alginate lyase Algt, disulfide bonds in molecules are analyzed, the alginate lyase is mutated, and the obtained alginate lyase mutant Amut8C has heat resistance, acid and alkali resistance and digestive enzyme tolerance, and compared with the alginate lyase Algt, the alginate lyase mutant Amut8C has the advantages that the content of the alginate lyase mutant Amut8C is increased, and the content of the alginate lyase mutant Amut8C is increased. The alginate oligosaccharide with the polymerization degree of 1-4 prepared from the alginate lyase mutant Amut8C under the same enzymolysis condition is higher in yield. The method can be applied to the fields of feeds, foods, detergents or biofuels.

The invention particularly relates to a pet food containing Chinese herbal medicines and capable of maintaining intestinal health of pets and a preparation method of the pet food. The pet food comprises an inner-layer structure and an outer-layer structure covering the surface of the inner-layer structure, wherein the inner-layer structure is a ball intermediate prepared by mixing Chinese herbal medicine powder, a nutritional additive and a binder; the outer layer structure comprises a meat source; the binder comprises a cartilage hydrolysate; the Chinese herbal medicine powder in the inner layer of the pet food is Chinese herbal medicine powder for improving gastrointestinal functions; the nutritional additive comprises inactivated probiotics, prebiotics, compound digestive enzymes, chicken liver hydrolyzed powder and auxiliary materials; by optimizing the formula and the process, the palatability of the pet food is ensured, the digestion and absorption conditions of pets are improved, the intestinal problems can be effectively prevented and treated, nutrition and drug therapy are integrated, and the overall health of the pets is promoted; good commercial application prospects are realized.

The invention discloses a cane molasses extract with a blood sugar reducing effect as well as a preparation method and application thereof, and belongs to the field of functional foods. According to the invention, cane molasses is subjected to dissolution, centrifugation, ultrafiltration impurity removal, desalination and nanofiltration concentration, and is mixed with calcium chloride, so that the cane molasses extract with the effect of reducing blood sugar is prepared. Experimental results prove that the cane molasses extract prepared by the invention has the characteristics of low glycemic index (GI), carbohydrate digestive enzyme inhibition, high temperature resistance, long-term storage resistance and the like, can also effectively repair pancreas and liver injury and regulate intestinal flora, and has a comprehensive hypoglycemic effect. The food with low glycemic index is prepared on the basis of the high sucrose carrier for the first time, and the food has sweet taste and the function of reducing blood sugar. The invention provides a new raw material scheme for the preparation of dietary supplement food and hypoglycemic drugs for hyperglycemia people, and has prominent practical application value and wide application prospect.

The invention discloses an aquatic product breeding feed capable of improving flavor and a preparation method thereof, and particularly relates to the technical field of breeding feeds, the feed comprises fish meal, soybean meal, soybean protein concentrate, shrimp meal, chicken meal, wheat flour, cuttlefish paste, phospholipid, grease, mineral substances, vitamins, lettuce polyphenol, an antioxidant, a mildew preventive and the like. Wherein the lettuce polyphenol is a key functional component. The preparation method comprises the following steps: pretreating the raw materials, mixing step by step, adding grease by spraying, tempering, granulating, drying, screening, spraying the antioxidant and the like. The lettuce polyphenol regulates and controls an mTOR signal channel to promote flavor amino acid deposition and inhibit protein degradation, meanwhile, the intestinal microecology is improved, the antioxidant capacity and digestive enzyme activity are enhanced, and the content of flavor substances such as glutamic acid and glycine in aquatic products is remarkably increased. The method is suitable for culturing various aquatic products, and can effectively improve the flavor and quality of the cultured aquatic products.

The invention provides application of bacillus tequilensis Bt-CO as a feed additive in largemouth bass culture, and belongs to the technical field of largemouth bass culture. The preservation number of the bacillus tequilensis Bt-CO is GDMCC No: 64960, the influence of the Bt-CO on the growth, survival, physical signs, immunity, antioxidant reaction, digestive enzyme, disease resistance, intestinal microbiota and metabolism of the largemouth bass is evaluated, and the probiotic potential of the Bt-CO and the effect of the Bt-CO in enhancing the resistance of the largemouth bass to aeromonas veronii infection are proved; bt-CO can be developed into a substitute of antibiotics, and a theoretical framework and a practical basis are provided for reasonable application of probiotics in aquaculture.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention belongs to the technical field of starch processing, and particularly relates to a preparation method of jack fruit seed resistant starch based on hydro-thermal treatment. Comprising the following steps: uniformly mixing jack fruit seed starch and plasma activated water, then gelatinizing, continuously stirring until the jack fruit seed starch is completely gelatinized, then adding gallic acid, continuously stirring, and after the reaction is finished, cooling, cleaning, drying and sieving to obtain the jack fruit seed resistant starch, wherein the gelatinization is carried out under the condition that the temperature is 80-100 DEG C. According to the method, the characteristic that plasma activated water is acidic is utilized, the jack fruit seed starch is induced to be combined with more polyphenols through hydrothermal treatment, more V-shaped compounds are formed, and the compounds have more resistant starch content, can effectively inhibit the activity of digestive enzymes and remarkably reduce the in-vitro digestibility of the jack fruit seed starch.

The present invention relates to an active oligopeptide RYIVPL, its preparation method and application. The active oligopeptide is RYIVPL, and its amino acid sequence is: Arg-Tyr-Ile-Val-Pro-Leu, which has the remarkable function of stimulating intestinal endocrine cells to secrete cholecystokinin (CCK). Compared with the prior art, the oligopeptide of the present invention can be artificially synthesized by a chemical solid-phase synthesis method, or can be obtained by enzymatic hydrolysis of wheat protein followed by separation and purification. The oligopeptide of the present invention has the characteristics of being safe, non-toxic and having no side effects, not being easily enzymolyzed by gastrointestinal digestive enzymes, and being easily absorbed, and is suitable as a functional component for developing drugs with the functions of delaying gastric emptying, promoting satiety and weight loss, and has great application value and broad prospects in the fields of medicine and the like.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The invention discloses a pharmaceutical composition for reducing methane emission of ruminants and improving feed digestibility and a preparation method of the pharmaceutical composition, and belongs to the technical field of feed additives. Comprising the following components in parts by weight: 35-45 parts of malt, 30-40 parts of mangnolia officinalis and 25-35 parts of rhizoma atractylodis. During preparation, the malt, the mangnolia officinalis and the rhizoma atractylodis are respectively crushed and sieved, and then the crushed malt, the crushed mangnolia officinalis and the crushed rhizoma atractylodis are fully mixed to obtain the pharmaceutical composition. On one hand, the antibacterial characteristics of the cortex magnoliae officinalis and the rhizoma atractylodis can remodel gastrointestinal tract microflora, the activity of microorganisms closely related to methane generation such as methanogens is accurately inhibited, and methane emission is reduced from the source; and on the other hand, digestive enzymes of malt directly act on feed nutrition decomposition, and magnolia officinalis and rhizoma atractylodis jointly construct an efficient digestion system by maintaining intestinal health and optimizing a digestion environment, so that the feed digestibility is remarkably improved, and growth, development and production performance improvement of ruminants are promoted.