341 results about "Digestive enzyme" patented technology

The invention relates to the technical field of pet health care, in particular to a probiotic composite additive for pet intestinal health and a preparation method of the probiotic composite additive. Comprising the following basic components in percentage by mass: 40% of lactobacillus complex, 10% of bifidobacterium complex, 10% of saccharomyces boulardii, 10% of prebiotic complex, 10% of natural digestive enzyme, 10% of functional plant extract, 10% of carrier and the like. 30% of a bifidobacterium complex group; 15% of saccharomyces boulardii; 8% of a prebiotic compound; 5% of natural digestive enzyme; and 2% of a functional plant extract. The multiple probiotics have a synergistic effect, the effect is better, the prebiotics promote proliferation of the probiotics and can enhance the effect of the additive, meanwhile, through drying treatment, the product is good in stability, long in shelf life and small in package design, the additive can be conveniently added to pet food, intestinal health of pets can be effectively improved, and the preparation method is simple and suitable for large-scale production.

The invention discloses a temperature-controllable preparation method for tissue temperature dissociation into single cells and application, the temperature-controllable preparation method for tissue temperature dissociation into single cells comprises the following steps: S1, providing a tissue sample, and pre-treating the tissue sample to obtain a sample block; s2, mixing the sample blocks with a compound digestive enzyme, and filtering to obtain a single-cell suspension after digestion treatment is completed; wherein the compound digestive enzyme is prepared from collagenase, hyaluronidase and papain; and selectively, S3, carrying out red removing treatment. The invention not only provides an improved preparation method of the single-cell suspension, but also has higher efficiency on the premise of ensuring the yield and activity of single cells, and provides a new strategy and tool for cancer research and treatment by particularly enriching CSCs.

The invention discloses a BimBH3 analogue containing D-type amino acid and N-terminal fatty acid acylation, a preparation method of the BimBH3 analogue and application of the BimBH3 analogue as a PTPN1 inhibitor to development of hypoglycemic drugs. The structural general formula of the novel BimBH3 mimic peptide analogue is shown as a formula I: # imgabs0 #, wherein Raa2 is equal to D-His, D-Leu, D-Pro, D-Ser, D-Arg, D-Gln, D-Lys, D-Trp, D-Val, D-Tyr, D-Ala, Raa3 is equal to D-Gln, Raa7 is equal to D-Arg, and Raa8 is equal to D-Allo-Ile. The mimic peptide compound is derived from a core region of a Bim-BH3 structural domain, different D-type amino acid site-specific replacement modification and N-terminal conjugated hexadecanoic acid modification are adopted for amino acids at the second, third, seventh and eighth sites respectively, the BimBH3 mimic peptide modified by the D-type amino acid is more stable and has higher metabolic stability, and the target binding capacity and inhibitory activity of the BimBH3 mimic peptide can still be reserved or even improved. The BimBH3 mimic peptide compound shows an excellent PTPN1 inhibitor, the ability of resisting protease hydrolase and various digestive enzymes is also remarkably improved, and the BimBH3 mimic peptide compound has longer pharmacological action time and can play a potential long-acting hypoglycemic role through subcutaneous injection or oral administration. The compound is completely prepared by a polypeptide solid-phase synthesis method, and an obtained crude product is subjected to semi-preparative liquid chromatography purification and freeze-drying to obtain a target compound.

The present disclosure relates to single cell protein (SCP) products obtained from Cupriavidus necator that has been engineered to produce digestive enzymes or otherwise enhanced. The biomass produced by the C. necator and the SCP products that include it contain digestive enzymes that are not naturally produced by C. necator.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses a preparation process and application of magnolol-loaded plant exosomes, and relates to the technical field of plant exosomes. Magnolol is extracted from a magnolia officinalis plant material through ultrasonic assistance, the plant exosomes are obtained from fresh ginger by using an ultracentrifugation method, and the plant exosomes are further modified through palmitic acid and polyethylene glycol, so that the magnolol-loaded plant exosomes are obtained. And finally, preparing the magnolol-loaded plant exosome by virtue of an incubation method. The magnolol can be protected from being damaged by gastric acid and digestive enzymes through membrane structure wrapping of the plant exosome, the stability of the magnolol in the digestive tract is improved, and it is further verified through mouse experiments that the magnolol-loaded plant exosome can be beneficial to regulation of intestinal flora.

The invention discloses a lactobacillus plantarum MW 2406C, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number of the lactobacillus plantarum MW 2406C is CGMCC No. 33339. The inventor finds that the bacterial strain has better oxidation resistance, pathogenic bacterium inhibition and oxygen resistance, and can be used for effectively fermenting feed, pre-decomposing nutritional ingredients in the feed and improving the utilization efficiency of the feed; in addition, the strain can improve liver and intestine health and improve immunity by enhancing hepatopancreas antioxidant enzyme activity and intestinal digestive enzyme activity, improving intestinal barrier health, up-regulating antibacterial peptide level and reducing inflammatory factors. When the lactobacillus plantarum is used for preparing the fermented feed for the river crabs, the utilization efficiency of the compound feed by the river crabs is improved, the intestinal health of the river crabs is improved, and the disease resistance is improved.

The invention provides a poultry probiotic preparation prepared by fermenting selenium-resistant duck-origin lactobacillus plantarum. The poultry probiotic preparation is prepared by inoculating duck-origin lactobacillus plantarum with high selenium ion loading capacity into a culture medium and fermenting, the duck-origin lactobacillus plantarum with high load of selenium ions has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30182. The high-load selenium duck-origin lactobacillus plantarum obtained by domesticating and screening by adopting a method for gradually increasing the concentration of selenium ions in a culture medium contains small peptides, small molecular organic acids, digestive enzymes and the like. As the antibacterial peptide is rich in targeted antibacterial peptide, the antibacterial peptide has an obvious inhibition effect on harmful bacteria such as escherichia coli, salmonella, riemerella anatipestifer and the like. Other bioactive substances such as digestive enzyme contained in the feed can promote the growth and development of poultry and improve the product quality.

The invention discloses a hydrogel plugging umbrella for gastric perforation and a preparation method thereof. The hydrogel plugging umbrella comprises a plugging part, and fixing parts are integrally formed at the two ends of the plugging part. The excellent plugging effect is achieved through the triple synergistic effect, the space mechanical interlocking effect brought by the double-umbrella-shaped design provides the immediate plugging and continuous self-expansion compaction effect, and physical and chemical adhesion of a tissue-gel interface ensures that under the corrosive gastric juice environment and the dynamic mechanical load, the plugging effect is good, and the plugging effect is good. A safe and lasting gastric perforation sealing effect can still be provided; the caffeic acid modified chitosan component has unique proton adsorption and biomolecule capture characteristics and can effectively block permeation of digestive enzymes such as gastric acid and pepsase, the protection effect not only maintains the activity of endogenous growth factors in a wound microenvironment, but also creates conditions beneficial to tissue regeneration, and the wound healing effect is improved. Therefore, healing and repairing of gastric perforation are remarkably promoted.

The invention discloses application of bacillus subtilis Z-14 in prevention of spoilage of silage corn straws, and relates to the technical field of whole-plant corn straw silage. According to the invention, a bacillus subtilis strain Z-14 is screened, and the bacillus subtilis strain Z-14 has significant broad-spectrum inhibition of pathogenic fungi and activity of pathogenic bacteria, secretes protease, amylase, cellulase and other digestive enzymes, effectively inhibits the number of bacteria, fungi and escherichia coli in the silage corn straw, accelerates the reduction of the pH value of the silage corn straw, and improves the yield of the silage corn straw. The content of aflatoxin B1 in the silage corn is obviously reduced, the silage quality is improved, a foundation is laid for effectively inhibiting the spoilage of the silage corn straw, and the silage microbial additive has huge application potential.

The invention discloses nutrition-balanced, sugar-controllable, fat-reducing and staple food nutrition-improving medicated food and a preparation method thereof. The medicated food is prepared from the following raw material components: 5-10% of high-quality protein, 1-10% of high-quality fat-containing food raw materials, 0.8-15% of medicinal and edible traditional Chinese medicines, 5-15% of high-quality carbohydrates, 5-60% of dietary fibers, 2-10% of functional sugar and 1-2% of minerals. The formula food is directly cooked with rice to form rice and porridge or is directly mixed with flour to form cooked wheaten food, so that the contact degree of starch and digestive enzyme and the activity of glycosidase can be reduced, the starch hydrolysis rate can be reduced, and the generation of postprandial blood sugar can be delayed. The formula food is prepared from various functional food raw materials and medicinal and edible food materials, is safe and free of side effects, and can be taken for a long time. All the components supplement each other to achieve a synergistic effect, the glycemic index of a patient can be adjusted, diabetic complications of the patient are improved, and therefore disease rehabilitation of the diabetic patient is promoted.

The invention discloses a pet hair-removing food and a preparation method thereof, and relates to the technical field of pet supplies, the pet hair-removing food comprises the following specific components: cellulose components, fat lubricating components, probiotics and prebiotics, natural digestive enzymes, plant extracts, vitamins and minerals, and natural vegetable oil; the additives specifically comprise a low-density microcapsule controlled release lubricant, a bioactive intestinal coating compound enzyme, modified polymer hydrated fibers and a plant-derived antistatic component; the preparation is carried out based on the above. According to the pet hair-removing food and the preparation method thereof, a plurality of additives are introduced, and particularly, a low-density microcapsule technology is used, so that lubricating components can be slowly released in intestinal tracts of pets, a longer-time lubricating effect is realized, and smooth discharge of hair is remarkably improved. In addition, the added bioactive intestinal coating compound enzyme and plant source antistatic component further enhance the health condition and hair removal effect of the intestinal tract, and reduce the occurrence of hair ball accumulation.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The present invention provides a method for preparing exosomes derived from antler periosteal mesenchymal stem cells and their applications. After obtaining antler periosteal tissue, the tissue is placed in a DPBS culture dish containing antibiotics for further processing. The periosteal tissue is then minced and washed with DPBS until it is free of blood. The minced tissue is treated with digestive enzymes and incubated in a carbon dioxide (CO2) incubator to facilitate enzymatic digestion. Subsequently, alpha-MEM complete culture medium is added to further process the tissue. The digested tissue is filtered and centrifuged to isolate the primary periosteal cells, which are then counted and cultured in a suitable medium to obtain mesenchymal stem cells (MSCs) derived from antler periosteum. The cultured periosteal MSCs are further processed to isolate exosomes, which can be applied to promote the proliferation and repair of human fibroblasts, enhance skin quality, and stimulate hair growth.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention provides a bacillus subtilis strain and application thereof, the bacillus subtilis strain is named as bacillus subtilis Ya3.1, is preserved in China General Microbiological Culture Collection Center on July 7, 2025, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35019; the bacillus subtilis Ya3.1 is separated from intestinal tracts of healthy hybrid sturgeons, and can be used as a probiotic additive for aquaculture, especially for sturgeon culture. The bacillus subtilis Ya3.1 provided by the invention has high safety, strong stress resistance and efficient intestinal colonization ability, and can significantly improve the activity of sturgeon intestinal digestive enzymes, improve the growth performance of sturgeons, enhance the non-specific immunity of sturgeons, improve the intestinal flora structure and significantly improve the ability to resist aeromonas bacterial infection; and a special probiotic resource can be provided for sturgeon culture.

The invention belongs to the technical field of cell extraction, and particularly relates to a method for extracting cells by enzymolysis of vitreous bodies, which comprises the following steps: centrifuging a vitreous body sample at room temperature, discarding supernate, and adding digestive enzyme into precipitate; after adding digestive enzyme, digesting in a water bath for 30 minutes; and after the enzymolysis is finished, centrifuging the enzymatic hydrolysate at room temperature, discarding the supernate, and collecting the precipitate, namely the enzymolysis vitreous cells. According to the technical scheme, a simple and efficient vitreous body enzymolysis method is provided, and a large number of cells can be provided for follow-up research.

The invention belongs to the technical field of materials, discloses a preparation method and application of novel stomach and intestinal tract passing sustained-release tannic acid, and particularly discloses a stomach and intestinal tract passing enteric coating material which comprises a surfactant, sugar alcohol, hydrogenated grease, polyacrylic resin and a thickening agent. The stomach-passing enteric coating material does not use expensive commercially available enteric coating materials, but adopts common surfactants, sugar alcohol substances and other substances, so that the grease and the water can be better fused, and the grease can be better coated outside in the coating process. The stomach-passing enteric coating material can ensure that tannic acid is not damaged by gastric acid, reduces the influence on animal digestive enzymes, and can be slowly released in intestinal tracts to be absorbed and utilized.

The utility model relates to the technical field of in-vitro intestinal tract simulation, in particular to an animal in-vitro intestinal tract analogue simulation device which comprises a main body container, a plurality of intestinal tract simulation units are arranged in the main body container, and a feeding system, a circulating system and a control system are further arranged in the main body container. The feeding system, the circulating system and the control system are electrically connected with the intestinal tract simulation unit. The digestion process in the animal intestinal tract can be simulated more truly through the arrangement of the multiple intestinal tract simulation units, and each unit can independently simulate different digestion stages. Due to the integrated design of the feeding system, the circulating system and the control system, the flowing of food in the intestinal tract, the action of digestive enzymes and the intestinal tract movement can be effectively simulated. By arranging the wriggling control piece and the contraction and relaxation piece, wriggling movement in the intestinal tract can be simulated, and the authenticity of simulation is enhanced.

The invention relates to the technical field of aquaculture, in particular to a carboxyl fluorescent quantum dot loaded gene editing system and application thereof in aquatic animal gene editing. The invention provides a preparation method of a carboxyl fluorescent quantum dot loaded gene editing system, which comprises preparation of functionalized carboxyl fluorescent quantum dots and connection of the functionalized carboxyl fluorescent quantum dots and plasmid DNA to obtain the carboxyl fluorescent quantum dot loaded gene editing system, and the carboxyl fluorescent quantum dot loaded gene editing system can be subsequently used for aquatic animal fertilized egg gene editing or cell transfection. According to the method, safe and efficient egg shell digestive enzymes and nano materials are used for gene editing breeding of aquatic animals.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

According to the chitosan-coated gamma-cyclodextrin metal organic framework delivery system and the preparation method and application thereof, hippophae rhamnoides polyphenol serving as an active component is efficiently loaded through a hydrophobic cavity of the chitosan-coated gamma-cyclodextrin metal organic framework delivery system, and encapsulation and release of the hippophae rhamnoides polyphenol can be achieved through the hydrophilic surface of the chitosan-coated gamma-cyclodextrin metal organic framework delivery system; chitosan is adopted as a protective layer to coat the cyclodextrin metal organic framework and the sea-buckthorn polyphenol, so that the carrier and the active components are prevented from being damaged by gastric acid and small intestine digestive enzymes, and the sea-buckthorn polyphenol can be accurately positioned to the colon part through the targeted positioning effect of the chitosan, so that the treatment on the colitis is realized, and the treatment effect is good. The invention provides a more effective and safer inflammation treatment means.

The invention discloses application of bacillus subtilis Z-14 in improving aerobic stability of silage, and relates to the technical field of silage corn fermentation, and the bacillus subtilis Z-14 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20821. The bacillus subtilis strain Z-14 disclosed by the invention has antagonistic activity on various pathogenic fungi and pathogenic bacteria which are easy to pollute in an ensiling process, so that the silage is prevented from spoilage after a cellar is opened; meanwhile, digestive enzymes generated by the strain can reduce the content of acid detergent fibers and improve the digestion and absorption rate of the silage; furthermore, the strain can reduce the pH value of the silage and improve the stability of the quality of the silage, and the acting effect is lasting. The strain Z-14 is applied to silage processing, and a material basis and a theoretical basis are provided for efficient and safe production of silage.

The invention provides an angiotensin converting enzyme inhibitory peptide and application thereof. The amino acid sequence of the angiotensin converting enzyme inhibitory peptide is PVKP. The ACE inhibitory activity of the inhibitory peptide PVKP provided by the invention is obviously improved compared with that of the original inhibitory peptide LYPVK, and the IC50 value is reduced from 10.6 mu g / mL to 9.6 mu g / mL; the digestive enzyme stability of the inhibitory peptide PVKP provided by the invention and the digestive enzyme stability of the original inhibitory peptide LYPVK under the same concentration are improved. Molecular simulation docking verifies that the interaction force between the ACE inhibitory peptide PVKP and ACE is enhanced compared with that of an original inhibitory peptide LYPVK, and the interaction force between alkyl groups and the interaction between the alkyl groups and Zn < 2 + > of an ACE active center are increased. The intracellular antihypertensive activity of the ACE inhibitory peptide PVKP is further verified through cell experiments, and compared with the original inhibitory peptide LYPVK, the intracellular NO release content is increased, and the ET-1 content is reduced. The ACE inhibitory peptide PVKP is small in molecular weight and good in water solubility, and application of the ACE inhibitory peptide PVKP in development of products with the blood pressure lowering effect is facilitated.

The invention relates to the technical field of extracts, in particular to macromolecular dried longan pulp heteropolysaccharide with a blood sugar reducing characteristic and a preparation method and application thereof. The preparation method comprises the following steps: drying the longan pulp, homogenizing the dried pulp, adding distilled water, performing hot water extraction, filtering through gauze, and separating the dried longan pulp heteropolysaccharide with the blood sugar reducing characteristic and the molecular weight of more than 100kDa from the dried longan pulp by combining processes such as dialysis, ultrafiltration interception and the like. The dry longan pulp polysaccharide is extracted by adopting a hot water extraction method, the dry longan pulp polysaccharide is separated by adopting an ultrafiltration interception system device, the main component DLP of the dry longan pulp polysaccharide can inhibit starch digestive enzymes, model cell insulin resistance can be improved, and human intestinal flora composition can be changed. The beverage has the functions of reducing blood sugar and regulating intestinal flora.

The invention discloses a preparation method and application of a bovine tongue mucous membrane organoid, and relates to the technical field of organoid separation and culture. The preparation method of the ox tongue mucosa organoid comprises the following steps: treating fetal ox tongue tip tissues with digestive enzymes Dispase I, Collagenase II and Collagenase IV, separating ox tongue mucosa, cutting into pieces, treating again with pancreatin, and sieving to remove tissues and impurities; and continuously culturing by adopting a three-dimensional culture technology-matrigel embedding method and an organoid culture medium to obtain the final ox tongue mucous membrane organoid. The ox tongue mucous membrane organoid prepared by the preparation method disclosed by the invention has normal cell proliferation, differentiation and metabolism functions, and can be stably passaged under an in-vitro culture condition; the method can be widely applied to various related scientific researches such as FMDV infection mechanism research, drug screening and vaccine evaluation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The application provides a preparation method and application of oat active peptides for promoting GLP-1 secretion, wherein the oat active peptides contain any one or more of peptide segments NDQRGEII, KQGDVIALPA, PFVQQQQ and PSEQYQPYPEQQEPFVQ. The preparation method of the oat active peptides comprises enzymolysis, chemical synthesis or genetic engineering. The oat active peptides can significantly promote the secretion of GLP-1 by intestinal endocrine cells, and have the advantages of safe and non-toxic side effects, tolerance to gastrointestinal digestive enzyme hydrolysis and easy absorption. The application has simple operation, low requirement for equipment, is suitable for industrial large-scale production, and can be used for the development of new drugs for controlling blood sugar or food for assisting in controlling blood sugar, drugs for controlling body weight or food for assisting in controlling body weight and the like.

The invention provides an alginate lyase mutant Amut8C as well as a coding gene and application thereof, belongs to the technical field of gene engineering and enzyme engineering, and can solve the problem that existing alginate lyase cannot meet the requirements of severe reaction conditions such as high temperature and acidity in industrial application. The amino acid sequence of the alginate lyase mutant Amut8C disclosed by the invention is as shown in SEQ ID NO. 1. According to the space structure of the alginate lyase Algt, disulfide bonds in molecules are analyzed, the alginate lyase is mutated, and the obtained alginate lyase mutant Amut8C has heat resistance, acid and alkali resistance and digestive enzyme tolerance, and compared with the alginate lyase Algt, the alginate lyase mutant Amut8C has the advantages that the content of the alginate lyase mutant Amut8C is increased, and the content of the alginate lyase mutant Amut8C is increased. The alginate oligosaccharide with the polymerization degree of 1-4 prepared from the alginate lyase mutant Amut8C under the same enzymolysis condition is higher in yield. The method can be applied to the fields of feeds, foods, detergents or biofuels.