123 results about "Digestive enzyme" patented technology

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The application provides a preparation method and application of oat active peptides for promoting GLP-1 secretion, wherein the oat active peptides contain any one or more of peptide segments NDQRGEII, KQGDVIALPA, PFVQQQQ and PSEQYQPYPEQQEPFVQ. The preparation method of the oat active peptides comprises enzymolysis, chemical synthesis or genetic engineering. The oat active peptides can significantly promote the secretion of GLP-1 by intestinal endocrine cells, and have the advantages of safe and non-toxic side effects, tolerance to gastrointestinal digestive enzyme hydrolysis and easy absorption. The application has simple operation, low requirement for equipment, is suitable for industrial large-scale production, and can be used for the development of new drugs for controlling blood sugar or food for assisting in controlling blood sugar, drugs for controlling body weight or food for assisting in controlling body weight and the like.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention belongs to the technical field of starch processing, and particularly relates to a preparation method of jack fruit seed resistant starch based on hydro-thermal treatment. Comprising the following steps: uniformly mixing jack fruit seed starch and plasma activated water, then gelatinizing, continuously stirring until the jack fruit seed starch is completely gelatinized, then adding gallic acid, continuously stirring, and after the reaction is finished, cooling, cleaning, drying and sieving to obtain the jack fruit seed resistant starch, wherein the gelatinization is carried out under the condition that the temperature is 80-100 DEG C. According to the method, the characteristic that plasma activated water is acidic is utilized, the jack fruit seed starch is induced to be combined with more polyphenols through hydrothermal treatment, more V-shaped compounds are formed, and the compounds have more resistant starch content, can effectively inhibit the activity of digestive enzymes and remarkably reduce the in-vitro digestibility of the jack fruit seed starch.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The application provides a freezing and recovery method and application of pluripotent stem cell induced differentiation middle stage cells, and the freezing method comprises the following steps: (1) the starting cells are pluripotent stem cells, which are induced to the middle stage; (2) the cells are recovered and counted by using a mild digestion enzyme, and the cells are resuspended in a freezing solution; (3) after programmed cooling to-80 DEG C, the cells are transferred into liquid nitrogen for storage, and are ready for use. The freezing method and the freezing solution provided by the application significantly improve the freezing survival rate of the differentiation middle stage cells, avoid the apoptosis or irreversible stress damage induced by the traditional freezing solution, and obviously improve the problems of cell damage and low survival rate caused by the general freezing solution in the prior art. The differentiation potential of the recovered cells is good, and the cells can successfully form mature kidney unit structures, so that the problem of subsequent differentiation failure in the prior art is solved.

The application relates to the field of second-generation sequencing, in particular to an ultrahigh-throughput multiplex PCR amplicon capture method for second-generation sequencing. The NGS capture method comprises the following steps: 1) performing multiplex PCR amplification on a sample to be detected by using a digestible composite modified primer pair, to obtain a PCR product; 2) performing an enzyme cutting reaction on the PCR product by using a second enzyme mixture containing a primer digestion enzyme, a mismatch correction enzyme and a terminal repair enzyme, including removing the excess primer part on the amplification product by using the digestion enzyme, and removing the non-specific amplification product by using the mismatch correction enzyme; 3) performing a ligation reaction by using a full-length adapter containing an index, to directly obtain a sequencing library or obtain a sequencing library after amplification. The method significantly improves the amplification efficiency and specificity, and has the advantages of simple steps, low cost, short time, wide application value in the fields of genetic disease detection, tumor companion diagnosis and genetic detection and scientific research.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The application belongs to the technical field of tissue dissociation, and discloses a tissue dissociation assembly and device. The tissue dissociation assembly comprises a dissociation bottle body, a dissociation bottle cap and a rotor. The dissociation bottle body is used for containing samples. The bottom of the dissociation bottle body is provided with a first shearing part. The dissociation bottle cap is detachably connected with the dissociation bottle body. The first end of the rotor is rotationally connected with the dissociation bottle cap. The second end of the rotor extends to the bottom of the dissociation bottle body and is provided with a second shearing part. The tissue dissociation assembly provided by the application is characterized in that, when the rotor rotates forward, the first side of the first shearing part interacts with the second shearing part to cut the samples, and at the same time, the digestive enzymes in the digestive solution in the dissociation bottle body digest the samples. Similarly, when the rotor rotates reversely, the second side of the first shearing part interacts with the second shearing part to stir the samples, so that the samples and the digestive solution are fully mixed, which is beneficial to the digestion of the samples, effectively avoids cross contamination, and improves the digestion efficiency.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The invention discloses Rhodosporidium toruloides for aquatic feed and application of the Rhodosporidium toruloides to aquaculture, relates to the technical field of microorganisms, and particularly relates to Rhodosporidium toruloides Ho7-3 which can be applied to aquaculture, has the effects of improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system, and can be used for improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system. The growth performance of cultured fishes can be obviously improved.

The invention discloses a graded preparation method of rice flour capable of promoting nutrient absorption based on digestive function development of infants. According to the invention, high-liquification and high-saccharification type, medium-liquification and high-saccharification type and low-liquification and medium-saccharification type enzymolysis rice flour is simulated and optimized by adopting models with different gastric acid pH values, digestive enzyme systems and gastrointestinal motility rules for the first time. According to the method, three parameters, namely different rice flour raw materials, gelatinization and composite enzymolysis parameters and different granularities, are combined and regulated, so that a rice flour product which is highly matched with gastric acid environments, amylase activity and intestinal tolerance in different developmental stages of infants is constructed. According to the method disclosed by the invention, the absorption efficiency and the energy utilization effect of carbohydrates in the body of the infant are remarkably improved, the digestive dynamic characteristics of the infant are scientifically adapted, the digestion problem caused by mismatching of starch composition and intestinal functions is effectively avoided, the risks of abdominal distension, diarrhea and constipation are remarkably reduced, and efficient utilization and healthy metabolism of nutrients are guaranteed.

This invention discloses a method and apparatus for modeling the gastric emptying characteristics of compressed dry food. The method includes: obtaining a set of gastrointestinal digestive enzyme concentration ranges; the set of gastrointestinal digestive enzyme concentration ranges includes salivary amylase concentration range, pepsin concentration range, and pancreatic enzyme concentration range; based on the set of gastrointestinal digestive enzyme concentration ranges, performing measurement processing on the compressed dry food to obtain a set of gastric retention rate values; and performing gastric emptying characteristic modeling on the set of gastric retention rate values ​​to obtain a set of gastric emptying models.

The present application belongs to the technical field of feed, and particularly relates to a broiler feed with reduced soybean meal dosage. The broiler feed with reduced soybean meal dosage comprises fermented distillers grains (FDG) with a mass fraction of 5% to 10%, and a residual basic daily ration; the basic daily ration comprises the following components in percentage by mass: corn 58.88%; soybean meal 31.50%; soybean oil 5.00%; stone powder 1.30%; salt 0.30%; calcium hydrogen phosphate 1.97%; DL-lysine 0.30%; DL-methionine 0.25%; premix 0.50%. It is found that fermentation can improve the feed nutritional value and apparent digestibility of distillers grains, and increase the addition amount of distillers grains in broilers; and when the addition amount reaches 10%, the liver and kidney functions, antioxidant capacity and digestive enzyme activity of the broilers are not obviously affected.

The present invention relates to a liposome composition for oral administration, the composition comprising a GLP-1 analog. The liposome according to the present invention has both excellent encapsulation efficiency and encapsulation content of the GLP-1 analog drug, and the prepared liposome composition resists digestive enzymes in the gastrointestinal tract and does not easily degrade when administered orally, thus having excellent stability, and has a high absorption rate in the body, and thus exhibits excellent pharmacological effects.

The application discloses a kind of based on NPFR gene's apple snail repellent and its application, belong to the field of agricultural pest control.This application is based on RNAi-mediated NPFR gene silencing technology, design and synthesis based on NPFR gene dsNPFR, by injecting dsNPFR into apple snail, lethal effect is obvious and NPFR gene expression, weight growth rate, leaf feeding area, digestive enzyme activity etc.are significantly reduced, effectively inhibit apple snail feeding and growth, the present application provides new strategy and molecular target for RNAi biological pesticide research and development, provides new technical approach for the prevention and control of apple snail invasion hazard.

The invention provides application of rhodopseudomonas palustris in improvement of oxidation resistance, meat quality and liver and intestine health of largemouth bass, and belongs to the technical field of aquaculture. The rhodopseudomonas palustris or the feed containing the rhodopseudomonas palustris is applied to improving the meat quality of the largemouth bass and / or improving the fish meat taste of the largemouth bass. Meanwhile, the invention also provides application of the rhodopseudomonas palustris in preparation of a product for improving the oxidation resistance of the largemouth bass or maintaining the health of the liver and / or the intestinal tract of the largemouth bass. The Rhodopseudomonas palustris not only can significantly improve the muscle quality of Micropterus salmoides, but also can improve serum and liver oxidation resistance, improve liver health, improve intestinal digestive enzyme activity, regulate intestinal flora and improve intestinal health, and the Rhodopseudomonas palustris has the best effect when being added into Micropterus salmoides feed in the form of living bacteria.

The invention relates to the technical field of aquaculture and feed engineering, and discloses a bionic fish digestion simulation system based on heat pump temperature control and software driving, which comprises an environment simulation subsystem, a bionic stomach digestion subsystem, a bionic pylorus subsystem, a bionic intestinal tract subsystem and an intelligent control and detection subsystem. The whole bionic digestive organ is immersed in an extremely stable water body environment, and accurate and stable control of the water temperature of + / -0.1 DEG C of the experimental water tank is realized. The problems that a traditional method is poor in temperature uniformity and large in fluctuation are fundamentally solved, the body temperature requirements of various fishes such as cold water fishes and warm water fishes can be accurately met, a reliable temperature basis is provided for digestive enzyme activity simulation, and the scientificity and repeatability of experimental data are remarkably improved.

1. Name of the designed product: medicine package box (digestive enzyme tablet). 2. Use of the designed product: medicine package box. 3. Design points of the designed product: in the pattern. 4. Picture or photo that best indicates the design points: front view.

Methods for performing non-reduced peptide mapping analysis of a protein of interest can include alkylating a protein of interest with N-ethyl maleimide (NEM) or an NEM analog under denaturing conditions to form an alkylated protein of interest; digesting the alkylated protein of interest with at least one digestive enzyme to form a peptide digest, and subjecting the peptide digest to liquid-chromatography-mass spectrometry analysis. The methods can be performed under mildly alkaline conditions.

This invention provides a method and application for improving the intestinal health of broilers using earthworm powder, belonging to the field of animal nutrition and feed technology. This invention solves the problems of poor growth performance, impaired intestinal health, and soybean meal substitution caused by nutritional deficiencies in broiler mixed meal diets. It includes the following steps: (1) raising earthworms in a substrate formed by mixing fermented chicken manure and Chinese medicine residue at a mass ratio of 2:3, and adding 2000 ppm sodium citrate to the substrate, controlling the substrate humidity at 50% to 55% and the temperature at 23 to 25°C; (2) preparing earthworm powder by freeze-drying the earthworms obtained in step (1) after disinfection; (3) replacing part of the soybean meal with 5.0% to 5.5% of the protein such as earthworm powder in the mixed meal broiler diet, and feeding the broilers. This invention significantly improves the growth performance, feed conversion ratio, immune function, intestinal digestive enzyme activity, intestinal morphology and barrier function of broilers, and optimizes the cecal microbiota, ultimately achieving efficient reduction and substitution of soybean meal and healthy and efficient broiler breeding.

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.

The invention provides a Chinese herbal medicine leavening agent for improving the egg laying performance of hens in the later egg laying period as well as a preparation method and application of the Chinese herbal medicine leavening agent. The Chinese herbal medicine leavening agent is obtained by fermenting compound Chinese herbal medicines including motherwort, angelica sinensis, ligusticum wallichii, peach kernels, astragalus membranaceus and baked ginger with compound probiotics, namely bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum. The compound probiotics are adopted for solid-state fermentation of the compound Chinese herbal medicine, strain compatibility is reasonable, the process is simple and efficient, synergistic fermentation is achieved, and release of active ingredients of the Chinese herbal medicine can be effectively promoted; the Chinese herbal medicine leavening agent is added into hen feed in the later egg laying period in proportion, the fat metabolism of hens can be adjusted, the content of intestinal digestive enzymes and reproductive hormone can be increased, the ovarian function of laying hens can be improved, the egg laying performance and the egg quality can be improved, and the Chinese herbal medicine leavening agent has good application prospects.

The application belongs to the technical field of biological medicine, and specifically discloses a nano delivery system and a preparation method and application thereof. mEVs are used as a core to encapsulate a depressor peptide QIGLF, a positively charged K5 is used to form an intermediate layer, the structural stability of the system is enhanced, and cell uptake is promoted, finally, a negatively charged Pec is used to form an outer protective shell, the stability and anti-enzymatic ability of the nanoparticles in the gastrointestinal environment are improved, and a core-shell multi-layer structure is constructed. The Pec can remain stable in the gastrointestinal tract, resist the destruction of gastric acid and digestive enzymes, and realize the responsive release of the core and the depressor peptide through specific degradation of the intestinal flora in the colon environment, and the K5 further enhances the uptake of the nanoparticles by the colon epithelial cells, thereby ensuring the efficient absorption of the depressor peptide. The nano delivery system has the comprehensive advantages of structural stability, intestinal retention, colon targeting and biological functionality during oral delivery, and provides an innovative solution for the efficient and safe application of the oral depressor peptide.

The invention discloses a multi-layer polymer functionalized plant-source nano delivery system and a preparation method thereof. The multi-layer polymer functionalized plant-source nano delivery system comprises (a) a plant-source exosome-like nano vesicle core; (b) a bioactive substance entrapped in the plant-derived exosome-like nanovesicle core or combined with the plant-derived exosome-like nanovesicle core; and (c) a multilayer polymeric shell layer covering the plant-derived exosome-like nanovesicle core, the shell layer comprising at least one cationic polymer layer and at least one anionic polymer layer. According to the system, the physical and chemical stability of the PDEV carrier under simulated gastric acid and digestive enzyme and long-term storage conditions is remarkably improved, and aggregation of nano-particles and premature leakage of active substances are prevented. Through surface modification, the interaction between the nano system and intestinal epithelial cells is enhanced, and the cellular uptake efficiency and the transshipment capability of crossing the epithelial barrier are improved.

The application provides a composition for improving intestinal digestive enzyme activity and application thereof, and belongs to the technical field of animal medicine. The composition is composed of double-kidney polysaccharide, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of the double-kidney polysaccharide, the ginsenoside Rb1 and the ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5, preferably 90:5:5, and the intestinal digestive enzyme is specifically duodenal trypsin and amylase. Experimental results show that the composition can significantly synergistically improve the activities of the duodenal trypsin and amylase of laying hens, improve the feed utilization rate, thereby improve the egg production rate, improve the egg weight and the eggshell strength, and effectively improve the breeding benefit of the laying hens.

The invention discloses an efficient lactic acid bacteria strengthening method, a high-activity lactic acid bacteria strain and application thereof, and belongs to the technical field of microbial breeding. The method comprises the following steps: (1) carrying out physical mutagenesis on a lactic acid bacteria starting strain to construct a mutant library; and (2) after inoculating and culturing the mutant library, adding a composite screening agent in an interval, graded and gradient increasing manner, carrying out gradient stress culture for multiple rounds until a culture solution is clear, and separating to obtain a strengthened strain. The composite screening agent comprises at least three of an osmotic pressure stress agent, a digestive enzyme stress agent, a cholate stress agent, an organic solvent stress agent and a cell wall stress agent. By constructing a progressive domestication environment, mutants with excellent comprehensive performance can be efficiently and directionally enriched. According to the lactic acid bacteria strain obtained by the method, the growth speed, the acid resistance, the cholate resistance, the ethanol resistance and the functional enzyme activity are synchronized and remarkably improved.

The invention relates to the technical field of microorganisms, and provides a plant lactobacillus strain which is separated from an intestinal tract sample of wild fish in a Danjiangkou reservoir. Experiments prove that the strain has relatively high acid resistance and bile salt tolerance, and has certain bacteriostatic ability to vibrio parahaemolyticus, vibrio alginolyticus, aeromonas veronii, aeromonas cainea and aeromonas hydrophila. The invention also provides an aquatic feed composition comprising such a strain. The aquatic feed composition containing the DY-JY3 strain can promote growth of largemouth black bass, improve immunity and digestive enzyme activity and adjust intestinal flora, so that the aquatic feed composition has remarkable potential in the aspect of being a probiotic candidate strain in aquaculture application, and a new microbial intervention strategy is provided for anti-infection treatment of fishes. The strain and the composition provided by the invention have wide application prospects in the field of aquatic products.