201 results about "Digestive enzyme" patented technology

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The present invention provides a method for preparing exosomes derived from antler periosteal mesenchymal stem cells and their applications. After obtaining antler periosteal tissue, the tissue is placed in a DPBS culture dish containing antibiotics for further processing. The periosteal tissue is then minced and washed with DPBS until it is free of blood. The minced tissue is treated with digestive enzymes and incubated in a carbon dioxide (CO2) incubator to facilitate enzymatic digestion. Subsequently, alpha-MEM complete culture medium is added to further process the tissue. The digested tissue is filtered and centrifuged to isolate the primary periosteal cells, which are then counted and cultured in a suitable medium to obtain mesenchymal stem cells (MSCs) derived from antler periosteum. The cultured periosteal MSCs are further processed to isolate exosomes, which can be applied to promote the proliferation and repair of human fibroblasts, enhance skin quality, and stimulate hair growth.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention provides a bacillus subtilis strain and application thereof, the bacillus subtilis strain is named as bacillus subtilis Ya3.1, is preserved in China General Microbiological Culture Collection Center on July 7, 2025, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35019; the bacillus subtilis Ya3.1 is separated from intestinal tracts of healthy hybrid sturgeons, and can be used as a probiotic additive for aquaculture, especially for sturgeon culture. The bacillus subtilis Ya3.1 provided by the invention has high safety, strong stress resistance and efficient intestinal colonization ability, and can significantly improve the activity of sturgeon intestinal digestive enzymes, improve the growth performance of sturgeons, enhance the non-specific immunity of sturgeons, improve the intestinal flora structure and significantly improve the ability to resist aeromonas bacterial infection; and a special probiotic resource can be provided for sturgeon culture.

The invention discloses a preparation method and application of a bovine tongue mucous membrane organoid, and relates to the technical field of organoid separation and culture. The preparation method of the ox tongue mucosa organoid comprises the following steps: treating fetal ox tongue tip tissues with digestive enzymes Dispase I, Collagenase II and Collagenase IV, separating ox tongue mucosa, cutting into pieces, treating again with pancreatin, and sieving to remove tissues and impurities; and continuously culturing by adopting a three-dimensional culture technology-matrigel embedding method and an organoid culture medium to obtain the final ox tongue mucous membrane organoid. The ox tongue mucous membrane organoid prepared by the preparation method disclosed by the invention has normal cell proliferation, differentiation and metabolism functions, and can be stably passaged under an in-vitro culture condition; the method can be widely applied to various related scientific researches such as FMDV infection mechanism research, drug screening and vaccine evaluation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The application provides a preparation method and application of oat active peptides for promoting GLP-1 secretion, wherein the oat active peptides contain any one or more of peptide segments NDQRGEII, KQGDVIALPA, PFVQQQQ and PSEQYQPYPEQQEPFVQ. The preparation method of the oat active peptides comprises enzymolysis, chemical synthesis or genetic engineering. The oat active peptides can significantly promote the secretion of GLP-1 by intestinal endocrine cells, and have the advantages of safe and non-toxic side effects, tolerance to gastrointestinal digestive enzyme hydrolysis and easy absorption. The application has simple operation, low requirement for equipment, is suitable for industrial large-scale production, and can be used for the development of new drugs for controlling blood sugar or food for assisting in controlling blood sugar, drugs for controlling body weight or food for assisting in controlling body weight and the like.

The invention provides an alginate lyase mutant Amut8C as well as a coding gene and application thereof, belongs to the technical field of gene engineering and enzyme engineering, and can solve the problem that existing alginate lyase cannot meet the requirements of severe reaction conditions such as high temperature and acidity in industrial application. The amino acid sequence of the alginate lyase mutant Amut8C disclosed by the invention is as shown in SEQ ID NO. 1. According to the space structure of the alginate lyase Algt, disulfide bonds in molecules are analyzed, the alginate lyase is mutated, and the obtained alginate lyase mutant Amut8C has heat resistance, acid and alkali resistance and digestive enzyme tolerance, and compared with the alginate lyase Algt, the alginate lyase mutant Amut8C has the advantages that the content of the alginate lyase mutant Amut8C is increased, and the content of the alginate lyase mutant Amut8C is increased. The alginate oligosaccharide with the polymerization degree of 1-4 prepared from the alginate lyase mutant Amut8C under the same enzymolysis condition is higher in yield. The method can be applied to the fields of feeds, foods, detergents or biofuels.

The invention particularly relates to a pet food containing Chinese herbal medicines and capable of maintaining intestinal health of pets and a preparation method of the pet food. The pet food comprises an inner-layer structure and an outer-layer structure covering the surface of the inner-layer structure, wherein the inner-layer structure is a ball intermediate prepared by mixing Chinese herbal medicine powder, a nutritional additive and a binder; the outer layer structure comprises a meat source; the binder comprises a cartilage hydrolysate; the Chinese herbal medicine powder in the inner layer of the pet food is Chinese herbal medicine powder for improving gastrointestinal functions; the nutritional additive comprises inactivated probiotics, prebiotics, compound digestive enzymes, chicken liver hydrolyzed powder and auxiliary materials; by optimizing the formula and the process, the palatability of the pet food is ensured, the digestion and absorption conditions of pets are improved, the intestinal problems can be effectively prevented and treated, nutrition and drug therapy are integrated, and the overall health of the pets is promoted; good commercial application prospects are realized.

The invention discloses a cane molasses extract with a blood sugar reducing effect as well as a preparation method and application thereof, and belongs to the field of functional foods. According to the invention, cane molasses is subjected to dissolution, centrifugation, ultrafiltration impurity removal, desalination and nanofiltration concentration, and is mixed with calcium chloride, so that the cane molasses extract with the effect of reducing blood sugar is prepared. Experimental results prove that the cane molasses extract prepared by the invention has the characteristics of low glycemic index (GI), carbohydrate digestive enzyme inhibition, high temperature resistance, long-term storage resistance and the like, can also effectively repair pancreas and liver injury and regulate intestinal flora, and has a comprehensive hypoglycemic effect. The food with low glycemic index is prepared on the basis of the high sucrose carrier for the first time, and the food has sweet taste and the function of reducing blood sugar. The invention provides a new raw material scheme for the preparation of dietary supplement food and hypoglycemic drugs for hyperglycemia people, and has prominent practical application value and wide application prospect.

The invention discloses an aquatic product breeding feed capable of improving flavor and a preparation method thereof, and particularly relates to the technical field of breeding feeds, the feed comprises fish meal, soybean meal, soybean protein concentrate, shrimp meal, chicken meal, wheat flour, cuttlefish paste, phospholipid, grease, mineral substances, vitamins, lettuce polyphenol, an antioxidant, a mildew preventive and the like. Wherein the lettuce polyphenol is a key functional component. The preparation method comprises the following steps: pretreating the raw materials, mixing step by step, adding grease by spraying, tempering, granulating, drying, screening, spraying the antioxidant and the like. The lettuce polyphenol regulates and controls an mTOR signal channel to promote flavor amino acid deposition and inhibit protein degradation, meanwhile, the intestinal microecology is improved, the antioxidant capacity and digestive enzyme activity are enhanced, and the content of flavor substances such as glutamic acid and glycine in aquatic products is remarkably increased. The method is suitable for culturing various aquatic products, and can effectively improve the flavor and quality of the cultured aquatic products.

The invention provides application of bacillus tequilensis Bt-CO as a feed additive in largemouth bass culture, and belongs to the technical field of largemouth bass culture. The preservation number of the bacillus tequilensis Bt-CO is GDMCC No: 64960, the influence of the Bt-CO on the growth, survival, physical signs, immunity, antioxidant reaction, digestive enzyme, disease resistance, intestinal microbiota and metabolism of the largemouth bass is evaluated, and the probiotic potential of the Bt-CO and the effect of the Bt-CO in enhancing the resistance of the largemouth bass to aeromonas veronii infection are proved; bt-CO can be developed into a substitute of antibiotics, and a theoretical framework and a practical basis are provided for reasonable application of probiotics in aquaculture.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention belongs to the technical field of starch processing, and particularly relates to a preparation method of jack fruit seed resistant starch based on hydro-thermal treatment. Comprising the following steps: uniformly mixing jack fruit seed starch and plasma activated water, then gelatinizing, continuously stirring until the jack fruit seed starch is completely gelatinized, then adding gallic acid, continuously stirring, and after the reaction is finished, cooling, cleaning, drying and sieving to obtain the jack fruit seed resistant starch, wherein the gelatinization is carried out under the condition that the temperature is 80-100 DEG C. According to the method, the characteristic that plasma activated water is acidic is utilized, the jack fruit seed starch is induced to be combined with more polyphenols through hydrothermal treatment, more V-shaped compounds are formed, and the compounds have more resistant starch content, can effectively inhibit the activity of digestive enzymes and remarkably reduce the in-vitro digestibility of the jack fruit seed starch.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The application provides a freezing and recovery method and application of pluripotent stem cell induced differentiation middle stage cells, and the freezing method comprises the following steps: (1) the starting cells are pluripotent stem cells, which are induced to the middle stage; (2) the cells are recovered and counted by using a mild digestion enzyme, and the cells are resuspended in a freezing solution; (3) after programmed cooling to-80 DEG C, the cells are transferred into liquid nitrogen for storage, and are ready for use. The freezing method and the freezing solution provided by the application significantly improve the freezing survival rate of the differentiation middle stage cells, avoid the apoptosis or irreversible stress damage induced by the traditional freezing solution, and obviously improve the problems of cell damage and low survival rate caused by the general freezing solution in the prior art. The differentiation potential of the recovered cells is good, and the cells can successfully form mature kidney unit structures, so that the problem of subsequent differentiation failure in the prior art is solved.

The invention provides an antibacterial peptide prepared by utilizing copper-loaded lactobacillus plantarum, which is prepared by inoculating high-copper-loaded duck-origin lactobacillus plantarum into a culture medium for fermentation and then using fermentation liquor, and the preservation number of the copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The lactobacillus plantarum obtained through domestication can tolerate high-concentration copper ions, the lactobacillus plantarum obtained through domestication and breeding can stably grow, and metabiotics generated through fermentation of the lactobacillus plantarum contain small peptides, small molecular organic acids, digestive enzymes and the like. Due to the fact that the antibiotic peptide rich in the afterbiogen has an obvious inhibiting effect on harmful bacteria of waterfowls, the prepared antibiotic peptide preparation effectively improves the antibacterial activity, and as a feed additive, the antibiotic peptide preparation is beneficial to elimination of inflammatory factors of intestinal tracts of poultry, maintenance of intestinal health, increase of the utilization rate of feed and improvement of the product quality.

The invention provides a preparation method and application of a pilose antler periosteum mesenchymal stem cell exosome.The preparation method comprises the steps that after pilose antler periosteum tissue is obtained, the periosteum tissue is put into a DPBS culture dish containing antibiotics for standby application, then the periosteum tissue is cut up, DPBS is used for cleaning till no blood exists, digestive enzymes are added into the cut periosteum tissue, and the pilose antler periosteum mesenchymal stem cell exosomes are obtained. The preparation method comprises the following steps of: reacting in an incubator containing carbon dioxide to decompose periosteum tissues, adding the periosteum tissues into an alpha-MEM complete culture solution, filtering and centrifuging digested and separated periosteum tissue cells to obtain primary periosteum cells at the bottom layer, and culturing the counted primary periosteum cells by using a culture solution to obtain the mesenchymal stem cells of the pilose antler periosteum, and culturing and filtering the mesenchymal stem cells of the pilose antler periosteum to obtain the exosome of the mesenchymal stem cells of the pilose antler periosteum, which is used for promoting proliferation and repair of human fibroblasts, improving skin quality, promoting hair growth and the like.

The application relates to the field of second-generation sequencing, in particular to an ultrahigh-throughput multiplex PCR amplicon capture method for second-generation sequencing. The NGS capture method comprises the following steps: 1) performing multiplex PCR amplification on a sample to be detected by using a digestible composite modified primer pair, to obtain a PCR product; 2) performing an enzyme cutting reaction on the PCR product by using a second enzyme mixture containing a primer digestion enzyme, a mismatch correction enzyme and a terminal repair enzyme, including removing the excess primer part on the amplification product by using the digestion enzyme, and removing the non-specific amplification product by using the mismatch correction enzyme; 3) performing a ligation reaction by using a full-length adapter containing an index, to directly obtain a sequencing library or obtain a sequencing library after amplification. The method significantly improves the amplification efficiency and specificity, and has the advantages of simple steps, low cost, short time, wide application value in the fields of genetic disease detection, tumor companion diagnosis and genetic detection and scientific research.

This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically involving a high-enzyme-active Liu Shen Qu (a type of fermented medicinal herb) and its fermentation process. The process first processes red beans, bitter almonds, artemisia annua, cocklebur, and polygonum hydropiper into a medicinal porridge, which is then mixed with wheat flour and a specific fermenting agent to form a soft mass. Its innovation lies in the use of a Bacillus subtilis trehalose protective agent complex and Aspergillus oryzae chitosan microspheres. These two fermenting agents are prepared by spray drying encapsulation and ionogel methods, respectively, significantly improving the survival rate and stability of the fermenting microorganisms during processing and storage. The soft mass undergoes two stages of precisely controlled temperature and humidity fermentation, and finally, after shaping and segmented drying, the finished product is obtained. This process effectively solves the problems of low enzyme activity and poor stability in traditional Liu Shen Qu fermentation, resulting in a Liu Shen Qu product with high digestive enzyme activity and stable, uniform quality.

The application belongs to the technical field of tissue dissociation, and discloses a tissue dissociation assembly and device. The tissue dissociation assembly comprises a dissociation bottle body, a dissociation bottle cap and a rotor. The dissociation bottle body is used for containing samples. The bottom of the dissociation bottle body is provided with a first shearing part. The dissociation bottle cap is detachably connected with the dissociation bottle body. The first end of the rotor is rotationally connected with the dissociation bottle cap. The second end of the rotor extends to the bottom of the dissociation bottle body and is provided with a second shearing part. The tissue dissociation assembly provided by the application is characterized in that, when the rotor rotates forward, the first side of the first shearing part interacts with the second shearing part to cut the samples, and at the same time, the digestive enzymes in the digestive solution in the dissociation bottle body digest the samples. Similarly, when the rotor rotates reversely, the second side of the first shearing part interacts with the second shearing part to stir the samples, so that the samples and the digestive solution are fully mixed, which is beneficial to the digestion of the samples, effectively avoids cross contamination, and improves the digestion efficiency.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The invention discloses Rhodosporidium toruloides for aquatic feed and application of the Rhodosporidium toruloides to aquaculture, relates to the technical field of microorganisms, and particularly relates to Rhodosporidium toruloides Ho7-3 which can be applied to aquaculture, has the effects of improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system, and can be used for improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system. The growth performance of cultured fishes can be obviously improved.

The invention discloses a graded preparation method of rice flour capable of promoting nutrient absorption based on digestive function development of infants. According to the invention, high-liquification and high-saccharification type, medium-liquification and high-saccharification type and low-liquification and medium-saccharification type enzymolysis rice flour is simulated and optimized by adopting models with different gastric acid pH values, digestive enzyme systems and gastrointestinal motility rules for the first time. According to the method, three parameters, namely different rice flour raw materials, gelatinization and composite enzymolysis parameters and different granularities, are combined and regulated, so that a rice flour product which is highly matched with gastric acid environments, amylase activity and intestinal tolerance in different developmental stages of infants is constructed. According to the method disclosed by the invention, the absorption efficiency and the energy utilization effect of carbohydrates in the body of the infant are remarkably improved, the digestive dynamic characteristics of the infant are scientifically adapted, the digestion problem caused by mismatching of starch composition and intestinal functions is effectively avoided, the risks of abdominal distension, diarrhea and constipation are remarkably reduced, and efficient utilization and healthy metabolism of nutrients are guaranteed.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

This invention discloses a method and apparatus for modeling the gastric emptying characteristics of compressed dry food. The method includes: obtaining a set of gastrointestinal digestive enzyme concentration ranges; the set of gastrointestinal digestive enzyme concentration ranges includes salivary amylase concentration range, pepsin concentration range, and pancreatic enzyme concentration range; based on the set of gastrointestinal digestive enzyme concentration ranges, performing measurement processing on the compressed dry food to obtain a set of gastric retention rate values; and performing gastric emptying characteristic modeling on the set of gastric retention rate values ​​to obtain a set of gastric emptying models.