The invention discloses a device for simulation of digestion in small intestine and a use method thereof. The device comprises a small intestine reactor, a stirrer, a water-bath control device, a computer monitoring system and a small intestine evacuation pump. A small intestinal fluid is added into the small intestine reactor, a solution or a dispersion liquid to be detected is added into the small intestine reactor, a simulator simulates small intestine peristalsis, the water-bath control device controls a temperature in the small intestine reactor, the computer monitoring system monitors an internal temperature, a pH value and digestion time of the small intestine reactor, the small intestine evacuation pump simulates small intestine evacuation, the digested product is collected, pancreatin and trypsin activity is eliminated, the digestion simulation process is repeated three times, and a digestion case of the material to be detected is detected. The device realizes first simulation of specific and detailed digestion in small intestine, realizes observation of digestion of various active materials or nutrients in the human small intestine, carries out simulation in a simulation environment designed according to a human physiological environment, has high conformity to the small intestine digestion and has high accuracy in simulation environment control.