167 results about "Lactobacillus casei" patented technology

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention relates to bacteria of the species Lactobacillus paracasei and / or compositions comprising Lactobacillus paracasei for use in preventing and / or treating a mental illness, a symptom affecting mental health and / or a condition associated with chronic stress, in a mammal. The invention further relates to methods and uses of said bacteria of the species Lactobacillus paracasei and / or compositions. The invention can be applied in restoring, maintaining, and / or promoting mental (including cognitive) health and has potential applications, inter alia, in the area of dietary and food supplements, medicaments and pharmaceuticals.

The invention relates to a polygonatum sibiricum probiotic compound preparation with an aging delaying effect and application thereof. The compound preparation comprises a polygonatum sibiricum extract and a probiotic composition, the bacterial strains in the probiotic composition comprise a combination of a bifidobacterium animalis subsp. Lactis BLa36 bacterial strain, a bifidobacterium longum BL21 bacterial strain, a bifidobacterium adolescentis BAC30 bacterial strain, a lactobacillus plantarum Lp90 bacterial strain, a lactobacillus casei LC89 bacterial strain, a bifidobacterium breve BBr60 bacterial strain and a pediococcus acidilactici CCFM7902 bacterial strain. After the rhizoma polygonati component and the probiotic component are combined for use, the rhizoma polygonati component and the probiotic component have potential synergistic effects in the aspects of improving the intestinal flora structure, regulating the intestinal environment, delaying senescence, reducing inflammation-related symptoms and the like. Moreover, further, various probiotic strains are compounded and combined for use, so that the capability of a related compound preparation in the aspect of delaying various symptoms of senescence can also be improved in a combined manner.

This invention discloses a raw material composition for preparing yogurt, yogurt, food, and a method for preparing the same. The raw material composition includes fermentation ingredients, additives, and a starter culture. The fermentation ingredients include 80-90 wt% soy milk; the additives include 0.1-0.4 wt% dietary fiber, 1-3 wt% natural starch, and 0.5-1.5 wt% coconut extract; the starter culture is used at 0.005-0.02 wt%, and includes *Streptococcus thermophilus*, *Lactobacillus delbrueckii* subsp. bulgaricus, *Lactobacillus casei*, and *Lactobacillus plantarum*. The yogurt of this invention has a good taste and flavor at room temperature, maintains a stable pH, and can be stored at room temperature for one month while ensuring the activity of lactic acid bacteria.

The invention belongs to the technical field of food fermentation, and provides a naan air-holding aroma-enhancing rapid fermentation composite leavening agent and a preparation method, the composite leavening agent comprises freeze-drying type lactobacillus plantarum, freeze-drying type saccharomyces cerevisiae, freeze-drying type pediococcus pentosaceus, lactobacillus rhamnosus, lactobacillus casei and Xiangba powder, and the optimal mass ratio of the freeze-drying type lactobacillus plantarum, the freeze-drying type saccharomyces cerevisiae, the freeze-drying type pediococcus pentosaceus, the lactobacillus rhamnosus, the lactobacillus casei and Xiangba powder is 2: 1: 3: 2: 3: 1 or 3: 1: 3: 1: 2: 2. The crusty pancake prepared according to the proportion has better sensory characteristics, specifically, the crusty pancake tastes softer and more elastic, the surface glossiness is improved, and the color is more uniform and natural. In addition, the method can also shorten the fermentation time, and endows the product with unique aroma of the sour dough fermented crusty pancake, thereby enhancing the flavor of the final product. The problems that the taste and the flavor are single when commercial yeast is independently used for making the fermented crusty pancake are solved, and the problems that when traditional sour dough is used for making fermented flour products, the fermentation time is long, the stability is poor, the quality is difficult to control, harmful microorganisms are prone to breeding and the like are solved.

The application discloses a fermented fruit juice with the effect of reducing high uric acid and a preparation method thereof, and comprises the following steps: step one, obtaining fruit wine after fruit juice fermentation, and obtaining de-alcohol fruit juice by removing alcohol from the fruit wine; step two, inoculating lactic acid bacteria into the de-alcohol fruit juice to perform lactic acid fermentation, and obtaining the fermented fruit juice; the lactic acid bacteria comprises lactobacillus helveticus CICC6064 and / or lactobacillus paracasei CICC20292. The fruit juice prepared by the fermentation process disclosed in the application not only retains the metabolic products of the yeast fermentation except for alcohol, but also increases the biomass and improves the content of apple polyphenol monomers through lactic acid bacteria fermentation, so that the fermented apple juice has the effects of rich taste and nutrition and reducing high uric acid, and has the innovation in the field of functional development of ordinary fruit juice.

The application provides a lactococcus lactis, a compound dairy product fermenting agent and application thereof, the lactococcus lactis has the functions of sweetening and flavoring, and is classified and named as lactococcus lactis Lactococcus lactis The strain has significant sweetening and flavoring characteristics, exhibits strong fructo-oligosaccharide (FOS) and acetaldehyde generation capacity in the fermentation process, can provide endogenous sweet taste and aroma flavor substances for fermented milk, and significantly improves the taste and flavor of the fermented milk.The lactococcus lactis C15 is compounded with lactobacillus casei AU9077 and lactobacillus rhamnosus AU9260 to obtain a compound dairy product fermenting agent, which has a significant synergistic effect in releasing fructo-oligosaccharide, continuously provides stable endogenous sweetening effect for fermented milk, improves the quality of dairy products, and meets the needs of consumers for healthy and flavored dairy products.

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

The present application belongs to the technical field of microbial fermentation feed, and discloses a fattening sheep complete mixed granular feed fermented by lactobacillus paracasei and a preparation method. The present application provides a lactobacillus paracasei strain for degrading gossypol, and the strain is used for solid fermentation of a mixture of cottonseed meal and cassava powder to obtain a fermented feed. The fermented feed is used for preparing a fattening sheep complete mixed granular feed, which can be directly used for feeding fattening sheep and can completely meet the nutritional needs of the fattening sheep. The complete mixed granular feed can significantly improve the palatability, and increase the feed intake and daily weight gain of the fattening sheep through fermentation of the cottonseed meal and the cassava powder.

The invention discloses a probiotic which is derived from human milk and has the effects of resisting oxidation, resisting inflammation, protecting tissues and protecting intestinal barriers and application of the probiotic. Lactobacillus paracasei DPUMW-M14-9 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CCTCC M 20252990. The strain has the characteristics of acid resistance, bile resistance, strong oxidation resistance, high surface hydrophobicity, self-aggregation capability and the like. The lactobacillus paracasei DPUMW-M14-9 can effectively adhere to epithelial cells, promote cell proliferation and regulate and control the process of a cell cycle. The bacterial strain can be used for inhibiting proinflammatory cytokines (TNF-alpha, IL-6 and IL-1beta) induced by lipopolysaccharide (LPS) and repairing tight junction proteins (ZO-1, Occludin and Claudin-1) at the same time, so that the integrity of epithelial cells is enhanced.

The present application belongs to the technical field of natural product extraction and preparation, and particularly relates to a preparation method of garlic polysaccharide and application thereof. The present application finds through experimental research that the garlic polysaccharide product prepared by taking garlic (such as black garlic and fresh garlic) as raw material not only has good antioxidant activity, but also has prebiotic effect and can effectively promote the growth of lactic acid bacteria such as lactobacillus plantarum and lactobacillus casei. In particular, the antioxidant and prebiotic effect of the black garlic polysaccharide product prepared by taking black garlic as raw material is better, so that the garlic polysaccharide product can be widely applied in the fields of food, medicine, daily chemical product and feed, and the comprehensive utilization value of garlic, especially black garlic, is effectively improved, and therefore the present application has wide and good practical application value and prospect.

The invention discloses a composite leavening agent and application of the composite leavening agent in high epiphytic microorganism and high lignocellulose straw silage and biomass pretreatment. The technical problem to be solved is how to improve the quality of silage obtained by fermenting silage raw materials (especially silage raw materials with high epiphytic microorganisms and / or high lignocellulose) and / or shorten the fermentation time of the silage and / or improve the cellulose conversion rate of the silage raw materials. The composite leavening agent provided by the invention is prepared from lactobacillus casei TD, lactobacillus plantarum JT-10, laccase and cellulase. The method can be used for fermentation of silage raw materials with high epiphytic microorganisms and high lignocellulose, the quality, nutritional value and the like of silage can be effectively improved, the conversion efficiency of biomass cellulose can be improved, and biological energy can be produced through pretreatment.

The invention discloses probiotic fermented carrot juice capable of improving intestinal health and resisting oxidation and a preparation method of a metagen. The carrot juice is prepared by fermenting carrot raw materials through a compound probiotic agent containing lactobacillus plantarum YZX21, lactobacillus casei YRL577, lactobacillus paracasei X11 and lactobacillus rhamnosus LR-C111. The preparation method comprises the following steps: cleaning, peeling, crushing and pulping carrots to prepare carrot primary pulp, then carrying out high-temperature instantaneous sterilization treatment on the carrot primary pulp, and carrying out vacuum concentration after sterilization; the concentrated carrot primary pulp is inoculated with the composite probiotic agent, the final inoculation concentration is 102-109 CFU / mL, standing fermentation is conducted for 9-15 hours at the temperature of 30-40 DEG C, the probiotic fermented carrot juice is obtained, and the probiotic fermented carrot juice is subjected to high-temperature sterilization treatment to obtain the postbiotics. The probiotic carrot juice and the metabiotics prepared by the invention have the functions of improving constipation, regulating intestinal flora and improving antioxidant capacity, and the preparation method is stable and efficient.

This invention relates to *Lactobacillus paracasei* NEFU-5 and its applications, belonging to the field of biotechnology. To address the problems of limited functionality and poor direct degradation of metabolites in existing probiotic strains, this invention provides *Lactobacillus paracasei* NEFU-5 (accession number CCTCC NO: M 2026147). This *Lactobacillus paracasei* NEFU-5 can simultaneously and efficiently degrade uric acid, triglycerides, and cholesterol, and possesses excellent probiotic properties and safety. The *Lactobacillus paracasei* NEFU-5 provided by this invention can be used to prepare fermented products and can be used to prevent or improve hyperuricemia, hyperlipidemia, and other concurrent metabolic diseases, showing significant application prospects.

The invention provides a culture medium for lactic acid bacteria after-generation and application, the culture medium takes trehalose as a carbon source and a yeast extract as a nitrogen source, so that the growth biomass of lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus can be remarkably increased, the pH is reduced, and the antibacterial ability is improved. Lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus are prepared through the culture medium for the lactobacillus after-generation, so that the lactobacillus after-generation has a good inhibition effect on main food-borne pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes; the preservative can be widely applied to preservation and preservation of meat products such as chilled chicken and pork. The preparation method of the lactic acid bacteria metagen is simple, convenient, low in cost, natural, safe and suitable for large-scale production.

The invention discloses a strain of chicken source lactobacillus casei H0001 and application of the chicken source lactobacillus casei H0001 in improvement of intestinal microecology of chicks. The chicken source lactobacillus casei provided by the invention is clear in source, high in host adaptability and suitable for colonization in the intestinal tract of a chick in the early stage; the strain provided by the invention can significantly improve the diversity of intestinal flora and the abundance of beneficial bacteria of chicks, and improve the intestinal micro-ecological structure; according to the invention, a safe and effective micro-ecological technical means is provided for early intestinal health regulation and control of chicks to replace or reduce the use of antibiotics; and (4) the method has a good application prospect and can be used for green and healthy breeding of poultry.

The invention provides a probiotic fermented traditional Chinese medicine preparation as well as a preparation method and application thereof. The probiotic fermented traditional Chinese medicine preparation is prepared by taking a water extract of a traditional Chinese medicine composition as a fermentation substrate and fermenting by utilizing probiotics, the traditional Chinese medicine composition comprises coix seeds, poria cocos, Chinese waxgourd seeds, chrysanthemum, selfheal, black beans, mung beans and liquorice. The probiotics comprise lactobacillus casei, lactobacillus reuteri, bifidobacterium animalis subsp. Lactis and lactobacillus rhamnosus; the probiotics comprise lactobacillus casei, lactobacillus reuteri, bifidobacterium animalis subsp. Lactis and lactobacillus rhamnosus; the traditional Chinese medicine composition has the effects of clearing damp, promoting diuresis, tonifying spleen, stopping diarrhea, clearing away heat and toxic materials, clearing liver and purging fire through combination of the medicines, so that the effect of removing damp toxin accumulation type stains is achieved; the traditional Chinese medicine raw material extracting solution is fermented through specific probiotics, macromolecular substances are decomposed, the bioavailability of traditional Chinese medicine active ingredients is improved, the probiotic fermented traditional Chinese medicine preparation has a good treatment effect on damp toxin accumulation type spots, the defect that the spots are difficult to cure and persistent and stubborn damp toxin spots are overcome, and the recurrence rate is reduced.

The invention discloses recombinant lactobacillus casei for expressing sialyltransferase ST6GALNAC1, and belongs to the technical field of genetic engineering and microorganisms. The recombinant bacterium is obtained by cloning an ST6GALNAC1 gene to a pPG612 vector and electrically transforming the ST6GALNAC1 gene into lactobacillus casei ATCC393. Experiments show that the engineering bacterium can significantly enhance glycosylation modification of intestinal mucoprotein MUC2, improve expression of tight junction proteins (ZO-1, Occludin and Claudin-1), inhibit activation of a p38 / MAPK inflammation pathway and regulate an intestinal flora structure, so that intestinal injury and systemic inflammatory response caused by salmonella are effectively relieved. The invention provides a strain basis and technical support for developing a novel oral microecological preparation.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The application belongs to the technical field of microbial fermentation, and particularly relates to a fermentation product of fruit and vegetable wastes and corn stalks and application thereof. The fermentation product is obtained by inoculating Aspergillus oryzae SLY-M-15 fermentation product and Saccharomyces cerevisiae into a first mixture containing crushed and air-dried corn stalks and fruit and vegetable wastes, performing aerobic fermentation, adding a second mixture containing crushed and air-dried corn stalks and fruit and vegetable wastes and corn cob, performing secondary aerobic fermentation, inoculating Lactobacillus casei, Lactobacillus acidophilus and Lactobacillus fermentum, and performing anaerobic fermentation. The obtained fermentation product is applied to animal feed, can improve the average daily weight gain of mutton sheep, reduce the feed conversion ratio, improve the tenderness and cooked meat rate of mutton, and increase the content of n-3 polyunsaturated fatty acids.

The application discloses a preparation method and application of a composite strain of a sugar-controlling probiotic, and belongs to the technical field of microorganisms. The method comprises the steps of strain stock solution preparation, anaerobic culture, pH value determination and regulation, and composite strain proportioning. Through a dynamic shunting mechanism with pH value 4.3 as a threshold, the culture solution lower than the value is subjected to re-acidification treatment, and the culture solution higher than the value is subjected to differential dilution and coating operation. Finally, Lactobacillus gasseri, Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus paracasei, Bifidobacterium breve, Bifidobacterium lactis and Bifidobacterium longum are mixed at a preset ratio, and a sustained-release agent is added for storage. The method can significantly improve the screening efficiency of target probiotics, the stability of the composite preparation and the blood glucose regulation effect, and can be widely applied to the development of sugar-controlling probiotic products.

The application belongs to the technical field of microorganisms, and discloses a lactic acid bacteria preparation for improving the yield and quality of waxy corn and application thereof. The active bacteria in the lactic acid bacteria preparation are Lactobacillus casei C-9 strains, and the strain preservation number of the C-9 strains is CGMCC No. 6734. The application is at least one selected from the following: A, improving the yield of waxy corn; B, improving the amylopectin content, soluble sugar content and total amino acid content in waxy corn kernels; C, improving the taste and flavor of waxy corn; and D, enhancing the stress resistance of waxy corn. The application uses Lactobacillus casei C-9 bacterial liquid for waxy corn planting, solves the problems of soil physicochemical property deterioration, soil microbial community imbalance, soil fertility decline and environmental pollution caused by chemical fertilizers through the combined mode of seed treatment, base fertilizer application, leaf surface spraying and root irrigation.

The application discloses a microbial fermentation method for improving the content of terpenoid components in Platycladi Cacumen and application, and specifically comprises the following steps: (1) substrate pretreatment: defatting, crushing and sterilizing Platycladi Cacumen to obtain fermentation substrate Platycladi Cacumen powder; (2) preparation of Lactobacillus casei seed liquid: inoculating Lactobacillus casei into MRS liquid medium for activation to prepare a logarithmic growth phase seed liquid; and (3) anaerobic fermentation: inoculating the Lactobacillus casei seed liquid into a fermentation system prepared from the Platycladi Cacumen powder, and performing static anaerobic fermentation to obtain a fermentation stock solution. The application adopts Lactobacillus casei as a fermentation strain for the first time, establishes a special fermentation method for directional enrichment of terpenoid components in Platycladi Cacumen, realizes the content of terpenoid components being improved from 1.2931 mg / mL to 3.8793 mg / mL, the improvement amplitude reaches 300%, the content of saponins is improved by 1.61 times, the content of GABA is up to 27.748 mg / mL, and the technical blank of directional enrichment of terpenoid components in Platycladi Cacumen in the prior art is filled.

This invention discloses a kidney-tonifying and liver-nourishing compound probiotic Cistanche fermented drink and its preparation method, comprising the following steps: mixing and sterilizing Cistanche deserticola, Poria cocos, malt, functional components, and natural selenium-strontium mineral water to obtain a fermentation substrate; activating *Lactobacillus casei*, *Lactobacillus acidophilus*, *Lactobacillus rhamnosus*, and *Lactobacillus plantarum* strains respectively, centrifuging and freeze-drying, and then mixing them to obtain a compound probiotic powder; culturing the compound probiotic powder in MRS medium to obtain a compound probiotic liquid; inoculating the compound probiotic liquid into the fermentation substrate for fermentation, sterilizing, and obtaining the kidney-tonifying and liver-nourishing compound probiotic Cistanche deserticola fermented drink. This invention, through the innovative integrated formulation technology of combining Cistanche deserticola, malt, and Poria cocos as medicinal and edible ingredients, multifunctional factor compounding, and compound probiotic fermentation, prepares a kidney-tonifying probiotic Cistanche deserticola fermented drink with high active ingredients, clear efficacy, and excellent flavor.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention discloses a production method of a green coffee bean extract capable of improving alpha-glucosidase inhibitory activity. Cellulase and pectinase are used for improving the yield of chlorogenic acid and a derivative (CGAs) of chlorogenic acid, tannase and esterase are used for conducting structural modification on polyphenol and CGAs in green coffee bean extract clear liquid, and then lactobacillus rhamnosus, lactobacillus paracasei and bacillus subtillis form a composite strain for fermentation. According to the method, the proportion of CGAs components and the proportion of caffeic acid in the green coffee bean extract are improved by combining an enzymic method with the probiotics to ferment the green coffee bean extract, and the alpha-glucosidase inhibitory activity of CGAs and metabolites thereof is improved.

The invention relates to a compound strain for preparing an ecological coagulator and application of the compound strain, the compound strain is composed of lactobacillus plantarum, lactobacillus casei and saccharomyces cerevisiae, the saccharomyces cerevisiae is subjected to gene introduction and recombination, and high-expression glutamine transaminase is obtained; the method for preparing the ecological coagulator from the compound bacterial strain comprises the following steps: collecting and standardizing bean clear liquid, sterilizing, inoculating and fermenting to prepare the ecological coagulator, applying the ecological coagulator to a bean product, curdling, squeezing and forming. The ecological coagulator has the advantages that a bean product curdled by the ecological coagulator contains GABA, soybean small molecule polypeptide and metabiotics, and is a bean product with nutritional and health-care effects, an exogenous chemical coagulator is not required to be added in the production process, the discharge of bean clear liquid can be effectively reduced by 30%, and clean production of the bean product is realized.

The invention belongs to the technical field of prebiotics, and particularly relates to plant extract composite prebiotics as well as a preparation method and application thereof. The invention provides a plant extract composite prebiotics, which is prepared by compounding a purslane ethanol extract and a common turnip ethanol extract in equal mass ratio, and the purslane ethanol extract and the common turnip ethanol extract are prepared by soaking purslane and common turnip in an ethanol solution with the volume concentration of 94-96%, performing ultrasonic extraction, concentrating and freeze-drying. The plant extract composite prebiotics provided by the invention can remarkably promote the growth of probiotics such as lactobacillus plantarum, lactobacillus paracasei and pediococcus pentosaceus, have the effects of regulating intestinal flora balance and promoting intestinal colonization of the probiotics, and also have antioxidant activity, thereby providing a new choice for development of prebiotics with natural sources.

A probiotic composition for treating or preventing metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma(MASLD-HCC) or colorectal cancer, comprises one or more microbiota selected from a group consisting of Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus GG, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis, Streptococcus thermophilus or any combination thereof. The probiotic composition demonstrates improved therapeutic effects when combined with low-dose Sorafenib for MASLD-HCC and outperforms 5-Fluorouracil in colorectal cancer models.