350 results about "Lactobacillus casei" patented technology

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus rhamnosus KF7 in improving intestinal barrier injury and regulating intestinal flora and functional food containing the lactobacillus rhamnosus KF7. The invention provides application of lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The preservation number of the lactobacillus rhamnosus KF7 is CGMCC (China General Microbiological Culture Collection Center) NO.6430. The invention further provides application of the lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The invention discloses the novel application of the caseus rhamnosus KF7 in the aspects of improving the normal intestinal epithelial barrier function, protecting the normal intestinal epithelial barrier from lipopolysaccharide induced barrier injury, recovering animal intestinal barrier injury caused by rhythm disorder, regulating intestinal flora disorder and improving growth, development and metabolic states for the first time. The method can be used for producing functional food products such as fermented milk, lactic acid bacteria beverages, probiotic powder, viable bacteria water, milk powder and cheese.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei ZY and application thereof, and the preservation number of the lactobacillus paracasei ZY is CGMCC (China General Microbiological Culture Collection Center) No.30303. The invention provides the lactobacillus paracasei ZY capable of regulating the iron balance of intestinal tracts, and the lactobacillus paracasei ZY also can promote the growth of saccharomyces cerevisiae. The animal feed prepared from the lactobacillus paracasei ZY can regulate the iron absorption of intestinal tracts according to the iron content in blood, promote the intestinal tracts to autonomously regulate the iron balance, and effectively solve the problem of iron balance imbalance in large-scale production; a fermented product prepared from the lactobacillus paracasei ZY is good in palatability, digestion is promoted, and the immunity of the organism is enhanced.

The invention provides a halite-containing probiotic preparation for improving gastrointestinal environment and a preparation method, the halite-containing probiotic preparation comprises the following components in parts by weight: 60-70 parts of a probiotic composition, the probiotic composition comprises lactobacillus casei Zhang, animal bifidobacterium subsp. Lactis V9, lactobacillus plantarum P-01, animal bifidobacterium subsp. Lactis B-01 and casei paracasei LC-11, the weight ratio is 3: 2: 2: 1: 1. 1.2 to 3.5 parts of nanoscale refined halite; 10 to 15 parts of a microcapsule embedding material; 0.6 to 1.2 parts of an antioxidant system; 15 to 30 parts of auxiliary materials; the probiotic preparation disclosed by the invention has the characteristics of obvious strain combination advantage, nano-scale halite protection and synergism, accurate release of a multi-layer microcapsule structure, synergistic effect of an antioxidant system and the like, the survival rate of probiotics, gastrointestinal tract tolerance and intestinal colonization capacity are remarkably improved, and a new technical scheme is provided for improving gastrointestinal health.

The present invention belongs to the technical fields of biological bacterium agents and functional food processing. Specifically, it relates to a bacterium agent for soybean milk fermentation and its application, a soy cheese stick and a preparation method thereof. The bacterium agent for soybean milk fermentation provided by the present invention uses Lactobacillus helveticus HL88 and Lactobacillus casei BL25 in combination. It can not only quickly acidify the soy milk, shortening the coagulation time of soy cheese, but also more fully hydrolyze soy proteins, releasing more short peptides and amino acids. In particular, it can highly produce γ-aminobutyric acid (GABA), enhancing the fermentation effect and nutritional value of soybean milk. Further, the soy cheese food prepared by fermenting with the above bacterium agent not only has a strong soy milk fragrance and a smooth texture, but is also easily digested and absorbed, improving the digestibility and reducing the soybean allergenicity, especially helping to improve exercise fatigue and enhance sleep quality. Therefore, the above bacterium agent provided by the present invention has excellent application prospects in the field of preparing functional foods for anti-fatigue and promoting sleep.

The present invention relates to the technical field of probiotics, and in particular to a strain of Lactobacillus casei ZKLc1221, its lactobacillus and its application in improving ulcerative colitis. Lactobacillus casei )ZKLc1221 has been deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on June 21, 2023, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 27673. The lactobacillus obtained by optimizing the fermentation conditions of Lactobacillus casei ZKLc1221 provided by the present invention has good thermal stability, good effect in improving ulcerative colitis, and can significantly improve pathological damage to the colon.

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention relates to the technical field of microorganisms, in particular to a probiotic composition for improving sleep and application, and the probiotic composition comprises lactobacillus paracasei BMC-2020, lactobacillus plantarum MCA-3066, lactobacillus rhamnosus, lactobacillus fermentum and lactobacillus casei. The probiotic composition provided by the invention is safe and efficient, has a prospect of developing a new medicine for improving sleep, and has a clinical practical value; the probiotic composition is used for fermenting the traditional Chinese medicine composition, the obtained traditional Chinese medicine fermentation composition contains probiotics and traditional Chinese medicine fermentation liquor, the probiotics and the traditional Chinese medicine fermentation liquor can effectively achieve a synergistic effect, and the sleep improving efficacy of a traditional Chinese medicine fermentation product is promoted; the traditional Chinese medicine fermented composition can reduce the awakening activity amount and the total awakening time, and has the effect of improving sleep.

The invention discloses a composite microecological preparation as well as a preparation method and application thereof. The compound microecological preparation disclosed by the invention comprises lactobacillus casei, lactobacillus plantarum and saccharomyces cerevisiae. The application effect of the composite microecological preparation in cow breeding is remarkable, the optimal addition amount of the composite microecological preparation is 100 mL / cow / day, the production performance of lactating cows can be improved, the milk yield is improved by 3.30 kg / cow / day, the milk yield of 4% standard milk is improved by 3.78 kg / cow / day, the total protein is improved by 15.9%, the albumin is improved by 14.7%, the globulin is improved by 16.5%, and the somatic cell number is reduced by 52.6%; and pollutant emission can be reduced, including 37.2% of ammonia emission reduction and 28.7% of carbon dioxide emission reduction.

The invention discloses application of lactobacillus paracasei S1 in relieving acute liver injury, and belongs to the technical field of microorganisms. The invention aims to provide application of lactobacillus paracasei S1 separated from fresh rumen fluid and applied to a product for relieving acute liver injury in relieving acute liver injury. The lactobacillus paracasei S1 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.33269. The lactobacillus paracasei S1 disclosed by the invention is a lactobacillus paracasei S1 strain. According to the lactobacillus paracasei S1 disclosed by the invention, AST, ALT, GGT and GDH activities of serum of mice with acute liver injury are reduced, and the condition of liver injury is improved. In conclusion, the lactobacillus paracasei S1 can be used for improving the acute liver injury or applied to feed, food and health care products, and a scientific basis is provided for developing functional products related to the lactobacillus paracasei S1 for improving the acute liver injury in the future.

The present invention belongs to the field of microbial technology, and specifically relates to a postbiotic composition for anti-corrosion and antibacterial in food, its preparation method and application. The raw materials of the postbiotic composition include: inactivated cells of a compound probiotic and metabolites of the compound probiotic; the compound probiotic includes Lactobacillus casei Zhang, Bifidobacterium animalis subsp. lactis V9, Lactobacillus plantarum P-8 and Lactobacillus plantarum PB-2. Through the mutual cooperation of the above four strains, the present invention can more effectively convert the culture medium substrate into several organic acids and short-chain fatty acids with more excellent anti-corrosion and antibacterial effects, improving the anti-corrosion and antibacterial effects of the postbiotic composition.

The object of the present invention is to use the strain of bacteria named Lacticaseibacillus paracasei DG® CNCM I-1572 DSM 34154 for use in a method of treating dysbiosis, particularly in patients with IBS (Irritable Bowel Syndrome), preferably without constipation, who have high fecal levels of Collinsella aerofaciens.

The invention provides a cordyceps militaris fermentation extract and application thereof in preservation of agaricus bisporus, and belongs to the technical field of deep processing of edible mushrooms and preservation of agricultural products. The preparation method of the cordyceps militaris fermentation extract comprises the following steps: (1) taking cordyceps militaris sporocarp, adding water, grinding, and performing enzymolysis by using cellulase and papain in sequence to obtain cordyceps militaris pulp; (2) adding glucose and waste beer yeast powder into the cordyceps militaris pulp, then inoculating lactobacillus paracasei and bifidobacterium bifidum, and fermenting; and (3) after fermentation is completed, carrying out ultrasonic crushing, centrifuging to remove thalli, and carrying out spray drying to obtain the cordyceps militaris fermentation extract. The method can effectively reduce the browning index and weight loss ratio of agaricus bisporus, can slow down the increase of malonaldehyde, reduces the activity of PPO enzyme, and prevents enzymatic browning caused by the synergistic effect of the PPO enzyme and peroxidase. The SOD enzyme activity in the agaricus bisporus can be kept, active oxygen in tissues can be removed, and the metabolic balance of the active oxygen can be maintained.

Provided is a composition for controlling uptake of microparticles into a body, the composition containing a lactic acid bacterium. The lactic acid bacterium is preferably any one selected from bacteria belonging to the genus Lactobacillus and bacteria belonging to the genus Streptococcus. For example, the lactic acid bacterium is a bacterium belonging to Lactobacillus delbrueckii, Lactobacillus paracasei, Lactobacillus gasseri, or Streptococcus thermophilus.

The invention discloses a method for fermenting sunflower bee pollen by using compound lactic acid bacteria and application of the sunflower bee pollen, and belongs to the technical field of fine and deep processing of bee products. The fermentation method comprises the following steps: inoculating compound lactic acid bacteria into sunflower bee pollen for fermentation; the preparation method of the compound lactic acid bacteria comprises the step of compounding lactobacillus plantarum and lactobacillus casei according to the volume ratio of (2 to 1) to (5 to 1), so as to obtain the compound lactic acid bacteria. The compound lactobacillus fermentation method suitable for the sunflower bee pollen is established for the first time, a new way is provided for high-value utilization of the bee pollen, and theoretical support is provided for breaking through the bottleneck of a bee pollen processing technology and developing an innovative bee pollen product with high absorptivity, high activity and pleasant flavor.

The invention relates to bacteria of the species Lactobacillus paracasei and / or compositions comprising Lactobacillus paracasei for use in preventing and / or treating a mental illness, a symptom affecting mental health and / or a condition associated with chronic stress, in a mammal. The invention further relates to methods and uses of said bacteria of the species Lactobacillus paracasei and / or compositions. The invention can be applied in restoring, maintaining, and / or promoting mental (including cognitive) health and has potential applications, inter alia, in the area of dietary and food supplements, medicaments and pharmaceuticals.

The invention belongs to the technical field of biology, and particularly relates to a composition with anti-hair-loss and hair-growing effects and a preparation method and application thereof. The preparation method provided by the invention comprises the following steps: standing and culturing composite lactobacillus consisting of lactobacillus plantarum, lactobacillus paracasei and lactobacillus bulgaricus according to a volume ratio of (12-15): (3-5): (4-8) to obtain a seed solution, further inoculating the seed solution into a fermentation culture medium containing polygonum multiflorum, ginseng leaves and cacumen biotae, standing and culturing to obtain a fermentation medium containing polygonum multiflorum, ginseng leaves and cacumen biotae; the composition with the anti-hair-loss and hair-growing effects is obtained. The composition prepared by the method disclosed by the invention is rich in flavone, polyphenol and other hair-growing and hair-loss-preventing active ingredients, and has good hair-loss-preventing, hair-growing, hair-brightening and hair-repairing effects.

The invention belongs to the related technical field of pet prescription food products, and particularly relates to a prescription food for pet dogs with acute intestinal inflammation and a preparation method of the prescription food, the prescription food comprises various components such as rice, barley, chicken powder and corn protein powder, and probiotics are self-made and comprise bifidobacterium, lactobacillus acidophilus and lactobacillus casei. The preparation method comprises the steps of quantitative raw material weighing, auxiliary agent grinding, constant-temperature accurate grinding, adding of specific components for accurate grinding again, heat preservation, deodorization and degassing, drop casting forming, cooling, packaging, sealed storage and the like. Compared with the prior art, the prescription food is high in safety, and feeding tests of healthy dogs have no abnormity; clinical cases show that the leucocyte number and the inflammatory factor content of sick dogs can be reduced; the intestinal barrier can be repaired, so that the intestinal mucosa repair is accelerated; intestinal flora can be improved, and the content of short-chain fatty acid in excrement is increased; the nutrition state can be maintained, and the weight loss range of sick dogs is reduced.

The invention relates to a polygonatum sibiricum probiotic compound preparation with an aging delaying effect and application thereof. The compound preparation comprises a polygonatum sibiricum extract and a probiotic composition, the bacterial strains in the probiotic composition comprise a combination of a bifidobacterium animalis subsp. Lactis BLa36 bacterial strain, a bifidobacterium longum BL21 bacterial strain, a bifidobacterium adolescentis BAC30 bacterial strain, a lactobacillus plantarum Lp90 bacterial strain, a lactobacillus casei LC89 bacterial strain, a bifidobacterium breve BBr60 bacterial strain and a pediococcus acidilactici CCFM7902 bacterial strain. After the rhizoma polygonati component and the probiotic component are combined for use, the rhizoma polygonati component and the probiotic component have potential synergistic effects in the aspects of improving the intestinal flora structure, regulating the intestinal environment, delaying senescence, reducing inflammation-related symptoms and the like. Moreover, further, various probiotic strains are compounded and combined for use, so that the capability of a related compound preparation in the aspect of delaying various symptoms of senescence can also be improved in a combined manner.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention relates to a composite strain leavening agent and application thereof in baking cake making. The composite strain leavening agent is composed of six microorganisms according to a specific ratio, including saccharomyces cerevisiae, lactobacillus rolfsii, lactobacillus curvatus, lactobacillus plantarum, acetic acid bacteria and associated lactobacillus casei, the optimal volume ratio of fresh and wet thalli of all the strains is 1: 0.3: 0.2: 0.1: 0.2: 0.2, and the viable count of all the strains is larger than or equal to 1.0 * 10 < 7 > CFU / g. According to the composite strain leavening agent, through the synergistic effect of a specific flora combination, the dual technical effects are achieved in the baked cake making process, on one hand, metabolites of different strains act together, and the texture characteristics of dough are remarkably improved; and on the other hand, various flavor substances generated by the composite flora endow the baked cakes with unique flavor characteristics. The method is of great significance in realizing industrial production of traditional leavening agent fermented food, inheriting ethnic diet culture and promoting and developing ethnic spirit.

The preparation method comprises the following steps: firstly fermenting milk, grain juice, pine needle juice and a sweetening agent by adopting lactobacillus plantarum, lactobacillus acidophilus and lactobacillus paracasei, and then adding saccharomycetes for secondary fermentation, so as to prepare a fermented beverage matrix; phospholipid, capric acid, cholesterol, pine needle extract and compound bacteria liquid are used as raw materials to load compound probiotic liposome of the pine needle extract, and then chitosan, sodium alginate and oat polysaccharide are adopted for secondary embedding and curing to prepare active probiotic particles. And assembling the fermented beverage matrix and the active probiotic particles to obtain the probiotic fermented beverage. The probiotic fermented beverage is high in product stability, can colonize viable probiotics in intestinal flora to play a health-care effect, and also has rich nutrition and taste.

The invention relates to the technical field of microorganisms, in particular to an anti-inflammatory compound probiotic and application thereof.Lactobacillus rhamnosus LGD-0817, lactobacillus rhamnosus LDL-1708 and lactobacillus plantarum LEP-1208 serve as basic dominant bacteria and then are compounded with multiple bacteria such as lactobacillus casei, lactobacillus delbrueckii and lactobacillus paracasei, the multiple bacteria can be matched and promoted mutually, and the anti-inflammatory compound probiotic has the anti-inflammatory effect. The traditional Chinese medicines jointly play a significant role in improving ulcerative colitis; the anti-inflammatory compound probiotics are used for traditional Chinese medicine fermentation, and the obtained traditional Chinese medicine fermentation composition can regulate intestinal flora, enhance the barrier function of the intestinal tract, inhibit release of proinflammatory factors, improve the shortening of the colon length of a mouse with colitis, inhibit the levels of proinflammatory cytokines IL-1beta, IL-6 and TNF-alpha, competitively inhibit colonization of pathogenic bacteria and improve the colonization of colitis. The method shows a good comprehensive intervention effect in a DSS-induced mouse ulcerative colitis model, and has clinical practical value.

The present invention belongs to the technical field of extracting effective pharmaceutical ingredients, and particularly relates to a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof. The composition comprises a spray-dried powder prepared from citrus fruit filtrate, Flammulina velutipes fiber, soy protein isolate, aronia berry extract, and black tea extract, and a compound probiotic, wherein the compound probiotic comprises Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei. The present invention adopts compound spraying of Flammulina velutipes fiber, soy protein isolate, aronia berry extract, black tea extract, and citrus fruit filtrate to improve the stability of total polyphenols and Vc in citrus fruits. The obtained citrus powder is further mixed with a compound probiotic to prepare a composition with pharmacologically active ingredients, which can effectively burn fat and lose weight and can be applied to weight loss drugs.

A postbiotic product of lactobacillus paracasei K56. The postbiotic product of a probiotic comprises inactivated bacteria of the probiotic and / or metabolites thereof, and the probiotic includes lactobacillus paracasei K56, which has the preservation number of CGMCC No.15139 or DSM27447; the metabolic products comprise: 62.60 mg / 100 g or more of L-methionine and 70.1 mg / 100 g or more of short peptide; and the inactivated bacteria of the probiotic are inactivated bacteria of a probiotic after undergoing fermentation, and the inactivation conditions for the inactivated bacteria involve treating at a temperature ranging from 70°C to 121°C for 5-15 min. The bacteriocin of lactobacillus paracasei K56 comprises exocytotic materials and metabolic products of lactobacillus paracasei K56, and in the bacteriocin, the mass ratio of citric acid to L-methionine is greater than 8:1. The postbiotic product of the probiotic can be applied to intestinal health.

The invention belongs to the technical field of microorganisms, and particularly discloses lactobacillus paracasei and application thereof, the lactobacillus paracasei is named as lactobacillus paracasei Jlus66, the lactobacillus paracasei is preserved in China General Microbiological Culture Collection Center on March 31, 2021, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.22101. The lactobacillus paracasei is named as lactobacillus paracasei Jlus66. The lactobacillus paracasei Jlus66 has the obvious beneficial effects in the treatment of the alopecia areata, and the obvious beneficial effects comprise the immune regulation, the anti-oxidation and anti-inflammatory effects and the optimization of the intestinal tract-skin axis. In addition, the medicine is high in safety and small in side effect, so that the medicine becomes a promising adjuvant therapy means.

To provide an external preparation for skin containing a plant-derived lactic acid bacterium and a specified plant extract.SOLUTION: An external preparation for skin comprises bacterial cells of Lactobacillus casei subsp. casei strain 327 and a specified plant extract.SELECTED DRAWING: None

The invention belongs to the technical field of soil improvement, and particularly relates to a saline-alkali soil conditioner as well as a preparation method and application thereof. The saline-alkali soil conditioner is a compound microbial agent for saline-alkali soil improvement. The compound microbial agent is prepared by jointly mixing and fermenting a seed solution of lactobacillus acidophilus, a seed solution of lactobacillus casei and a seed solution of streptococcus thermophilus; the volume ratio of the seed solution of the lactobacillus acidophilus to the seed solution of the lactobacillus casei to the seed solution of the streptococcus thermophilus is 1: (0.8-1.2): (0.8-1.2). The saline-alkali soil conditioner provided by the invention has the characteristics of quickly reducing alkaline harm of saline-alkali soil, promoting crop growth, improving nutrient absorption and utilization, improving microbial diversity and functional activity of soil, and being easy to apply, low in cost, quick in effect and the like.

The invention discloses lactobacillus casei capable of improving the flavor of a tea beverage through fermentation and application of the lactobacillus casei. The lactobacillus casei is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus casei is CGMCC No.32672, and the preservation date of the lactobacillus casei is November 18, 2024. The lactobacillus casei can be used for producing extracellular beta-glucosidase, polyphenol oxidase and peroxidase; when being used for fermentation, the fermentation broth can improve the flavor and color of a tea beverage, increase the content of organic acid, reduce the bitter taste of the tea beverage, generate new volatile aroma substances such as nerolidol, isobutanol, ethyl hexanoate, ethyl 3-hexenoate, ethyl caprylate, ethyl 2-hexenoate and the like, and enrich the aroma substance composition of the tea beverage. The invention provides a novel tea beverage production process which has the advantages of simplicity and convenience in operation, low cost, remarkable effect and the like, and is suitable for industrial production.

The invention belongs to the technical field of probiotics, and particularly relates to a lactobacillus casei MY01 strain with an effect of improving immunity and application of the lactobacillus casei MY01 strain. A new Lactobacillus casei MY01 strain is separated from feces of a healthy infant in Guangzhou city, Guangdong province, a zebra fish immunosuppression model is constructed, and research finds that the Lactobacillus casei MY01 strain can increase the number of macrophages, neutrophil and T cells, increase the expression quantity of if n phi 1, if n phi 3 and if ng genes, inhibit the activity of angiotensin converting enzyme, inhibit the expression quantity of if n is greater than 1, and inhibit the expression quantity of if n is greater than 1, so that the new Lactobacillus casei MY01 strain can be used for preparing the new Lactobacillus casei MY01 strain. The potential effect of improving the immunity is realized. Therefore, the strain can be used in the field of medicine, and can be used for developing immunomodulatory drugs and assisting in treating immunosuppression related diseases, such as chronic infection and tumors; the method can also be applied to the health care product industry, develops health care products for enhancing immunity, and helps people to improve autoimmunity and maintain a healthy state.

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.