243 results about "Lactobacillus casei" patented technology

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention discloses a composite microecological preparation as well as a preparation method and application thereof. The compound microecological preparation disclosed by the invention comprises lactobacillus casei, lactobacillus plantarum and saccharomyces cerevisiae. The application effect of the composite microecological preparation in cow breeding is remarkable, the optimal addition amount of the composite microecological preparation is 100 mL / cow / day, the production performance of lactating cows can be improved, the milk yield is improved by 3.30 kg / cow / day, the milk yield of 4% standard milk is improved by 3.78 kg / cow / day, the total protein is improved by 15.9%, the albumin is improved by 14.7%, the globulin is improved by 16.5%, and the somatic cell number is reduced by 52.6%; and pollutant emission can be reduced, including 37.2% of ammonia emission reduction and 28.7% of carbon dioxide emission reduction.

The object of the present invention is to use the strain of bacteria named Lacticaseibacillus paracasei DG® CNCM I-1572 DSM 34154 for use in a method of treating dysbiosis, particularly in patients with IBS (Irritable Bowel Syndrome), preferably without constipation, who have high fecal levels of Collinsella aerofaciens.

Provided is a composition for controlling uptake of microparticles into a body, the composition containing a lactic acid bacterium. The lactic acid bacterium is preferably any one selected from bacteria belonging to the genus Lactobacillus and bacteria belonging to the genus Streptococcus. For example, the lactic acid bacterium is a bacterium belonging to Lactobacillus delbrueckii, Lactobacillus paracasei, Lactobacillus gasseri, or Streptococcus thermophilus.

The invention relates to bacteria of the species Lactobacillus paracasei and / or compositions comprising Lactobacillus paracasei for use in preventing and / or treating a mental illness, a symptom affecting mental health and / or a condition associated with chronic stress, in a mammal. The invention further relates to methods and uses of said bacteria of the species Lactobacillus paracasei and / or compositions. The invention can be applied in restoring, maintaining, and / or promoting mental (including cognitive) health and has potential applications, inter alia, in the area of dietary and food supplements, medicaments and pharmaceuticals.

The invention belongs to the related technical field of pet prescription food products, and particularly relates to a prescription food for pet dogs with acute intestinal inflammation and a preparation method of the prescription food, the prescription food comprises various components such as rice, barley, chicken powder and corn protein powder, and probiotics are self-made and comprise bifidobacterium, lactobacillus acidophilus and lactobacillus casei. The preparation method comprises the steps of quantitative raw material weighing, auxiliary agent grinding, constant-temperature accurate grinding, adding of specific components for accurate grinding again, heat preservation, deodorization and degassing, drop casting forming, cooling, packaging, sealed storage and the like. Compared with the prior art, the prescription food is high in safety, and feeding tests of healthy dogs have no abnormity; clinical cases show that the leucocyte number and the inflammatory factor content of sick dogs can be reduced; the intestinal barrier can be repaired, so that the intestinal mucosa repair is accelerated; intestinal flora can be improved, and the content of short-chain fatty acid in excrement is increased; the nutrition state can be maintained, and the weight loss range of sick dogs is reduced.

The invention relates to a polygonatum sibiricum probiotic compound preparation with an aging delaying effect and application thereof. The compound preparation comprises a polygonatum sibiricum extract and a probiotic composition, the bacterial strains in the probiotic composition comprise a combination of a bifidobacterium animalis subsp. Lactis BLa36 bacterial strain, a bifidobacterium longum BL21 bacterial strain, a bifidobacterium adolescentis BAC30 bacterial strain, a lactobacillus plantarum Lp90 bacterial strain, a lactobacillus casei LC89 bacterial strain, a bifidobacterium breve BBr60 bacterial strain and a pediococcus acidilactici CCFM7902 bacterial strain. After the rhizoma polygonati component and the probiotic component are combined for use, the rhizoma polygonati component and the probiotic component have potential synergistic effects in the aspects of improving the intestinal flora structure, regulating the intestinal environment, delaying senescence, reducing inflammation-related symptoms and the like. Moreover, further, various probiotic strains are compounded and combined for use, so that the capability of a related compound preparation in the aspect of delaying various symptoms of senescence can also be improved in a combined manner.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention relates to the technical field of microorganisms, in particular to an anti-inflammatory compound probiotic and application thereof.Lactobacillus rhamnosus LGD-0817, lactobacillus rhamnosus LDL-1708 and lactobacillus plantarum LEP-1208 serve as basic dominant bacteria and then are compounded with multiple bacteria such as lactobacillus casei, lactobacillus delbrueckii and lactobacillus paracasei, the multiple bacteria can be matched and promoted mutually, and the anti-inflammatory compound probiotic has the anti-inflammatory effect. The traditional Chinese medicines jointly play a significant role in improving ulcerative colitis; the anti-inflammatory compound probiotics are used for traditional Chinese medicine fermentation, and the obtained traditional Chinese medicine fermentation composition can regulate intestinal flora, enhance the barrier function of the intestinal tract, inhibit release of proinflammatory factors, improve the shortening of the colon length of a mouse with colitis, inhibit the levels of proinflammatory cytokines IL-1beta, IL-6 and TNF-alpha, competitively inhibit colonization of pathogenic bacteria and improve the colonization of colitis. The method shows a good comprehensive intervention effect in a DSS-induced mouse ulcerative colitis model, and has clinical practical value.

To provide an external preparation for skin containing a plant-derived lactic acid bacterium and a specified plant extract.SOLUTION: An external preparation for skin comprises bacterial cells of Lactobacillus casei subsp. casei strain 327 and a specified plant extract.SELECTED DRAWING: None

This invention discloses a raw material composition for preparing yogurt, yogurt, food, and a method for preparing the same. The raw material composition includes fermentation ingredients, additives, and a starter culture. The fermentation ingredients include 80-90 wt% soy milk; the additives include 0.1-0.4 wt% dietary fiber, 1-3 wt% natural starch, and 0.5-1.5 wt% coconut extract; the starter culture is used at 0.005-0.02 wt%, and includes *Streptococcus thermophilus*, *Lactobacillus delbrueckii* subsp. bulgaricus, *Lactobacillus casei*, and *Lactobacillus plantarum*. The yogurt of this invention has a good taste and flavor at room temperature, maintains a stable pH, and can be stored at room temperature for one month while ensuring the activity of lactic acid bacteria.

The present invention relates to a process for producing a probiotic powder from grape marc, comprising the steps of: a) mixing 75-85% of grape marc and 15-25% of an inoculum, said inoculum consisting of Lactobacillus casei 431 and Bifidobacterium BB12; b) keeping the mixture from step a) under aseptic conditions for a time interval in the range of 24-168 hours; c) drying the mixture from step b) at a temperature in the range of 60-65°C until it reaches a constant weight; and d) grinding the dry mixture from step c). The invention pertains to the field of production of probiotic products.

The present application relates to a method for producing a fermented dairy product using Lactobacillus casei. In particular, the present invention relates to a method of producing a fermented dairy product comprising adding lactic acid bacteria to the milk wherein the bacteria comprise Lactobacillus casei and at least one other strain of lactic acid bacteria other than Lactobacillus casei, wherein the other strain has a lactose metabolism deficiency but is capable of metabolizing one or more carbohydrates other than lactose present in milk.

The present invention belongs to the field of microbial fermentation technology, and discloses a plant fermentation product with soothing and repairing effects, as well as its preparation method and application. The raw materials for preparing the plant fermentation product include: plant raw materials, solvents and fermentation strains; the plant raw materials include sea buckthorn fruit and licorice; the solvents include solvent A and solvent B, solvent A includes glycerol and / or ethoxydiglycol, and solvent B includes water; the fermentation strains include at least one of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum. The present invention organically combines high-pressure homogenization and specific microbial fermentation, and high-pressure homogenization is supplemented with a specific solvent, so that plant tissue cells can be fully broken, components can be fully released and dissolved, and the utilization efficiency of plant raw materials can be improved. The plant composite liquid obtained by high-pressure homogenization is directly used as the subsequent fermentation matrix. Through the biotransformation ability of microorganisms, a plant fermentation product with a high content of active substances and significant soothing and repairing effects can be prepared.

The invention belongs to the technical field of food fermentation, and provides a naan air-holding aroma-enhancing rapid fermentation composite leavening agent and a preparation method, the composite leavening agent comprises freeze-drying type lactobacillus plantarum, freeze-drying type saccharomyces cerevisiae, freeze-drying type pediococcus pentosaceus, lactobacillus rhamnosus, lactobacillus casei and Xiangba powder, and the optimal mass ratio of the freeze-drying type lactobacillus plantarum, the freeze-drying type saccharomyces cerevisiae, the freeze-drying type pediococcus pentosaceus, the lactobacillus rhamnosus, the lactobacillus casei and Xiangba powder is 2: 1: 3: 2: 3: 1 or 3: 1: 3: 1: 2: 2. The crusty pancake prepared according to the proportion has better sensory characteristics, specifically, the crusty pancake tastes softer and more elastic, the surface glossiness is improved, and the color is more uniform and natural. In addition, the method can also shorten the fermentation time, and endows the product with unique aroma of the sour dough fermented crusty pancake, thereby enhancing the flavor of the final product. The problems that the taste and the flavor are single when commercial yeast is independently used for making the fermented crusty pancake are solved, and the problems that when traditional sour dough is used for making fermented flour products, the fermentation time is long, the stability is poor, the quality is difficult to control, harmful microorganisms are prone to breeding and the like are solved.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

The application discloses a fermented fruit juice with the effect of reducing high uric acid and a preparation method thereof, and comprises the following steps: step one, obtaining fruit wine after fruit juice fermentation, and obtaining de-alcohol fruit juice by removing alcohol from the fruit wine; step two, inoculating lactic acid bacteria into the de-alcohol fruit juice to perform lactic acid fermentation, and obtaining the fermented fruit juice; the lactic acid bacteria comprises lactobacillus helveticus CICC6064 and / or lactobacillus paracasei CICC20292. The fruit juice prepared by the fermentation process disclosed in the application not only retains the metabolic products of the yeast fermentation except for alcohol, but also increases the biomass and improves the content of apple polyphenol monomers through lactic acid bacteria fermentation, so that the fermented apple juice has the effects of rich taste and nutrition and reducing high uric acid, and has the innovation in the field of functional development of ordinary fruit juice.

The application provides a lactococcus lactis, a compound dairy product fermenting agent and application thereof, the lactococcus lactis has the functions of sweetening and flavoring, and is classified and named as lactococcus lactis Lactococcus lactis The strain has significant sweetening and flavoring characteristics, exhibits strong fructo-oligosaccharide (FOS) and acetaldehyde generation capacity in the fermentation process, can provide endogenous sweet taste and aroma flavor substances for fermented milk, and significantly improves the taste and flavor of the fermented milk.The lactococcus lactis C15 is compounded with lactobacillus casei AU9077 and lactobacillus rhamnosus AU9260 to obtain a compound dairy product fermenting agent, which has a significant synergistic effect in releasing fructo-oligosaccharide, continuously provides stable endogenous sweetening effect for fermented milk, improves the quality of dairy products, and meets the needs of consumers for healthy and flavored dairy products.

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

The present application belongs to the technical field of microbial fermentation feed, and discloses a fattening sheep complete mixed granular feed fermented by lactobacillus paracasei and a preparation method. The present application provides a lactobacillus paracasei strain for degrading gossypol, and the strain is used for solid fermentation of a mixture of cottonseed meal and cassava powder to obtain a fermented feed. The fermented feed is used for preparing a fattening sheep complete mixed granular feed, which can be directly used for feeding fattening sheep and can completely meet the nutritional needs of the fattening sheep. The complete mixed granular feed can significantly improve the palatability, and increase the feed intake and daily weight gain of the fattening sheep through fermentation of the cottonseed meal and the cassava powder.

This invention discloses a water mold inhibitor, its preparation method, and its application. The water mold inhibitor comprises Agaricus bisporus polysaccharide, *Epicoccumnigrum* Z01, *Lactobacillus paracasei* LY02, and ergothioneine. The preservation number of *Epicoccumnigrum* Z01 is CCTCC NO: M2024967. The concentration of Agaricus bisporus polysaccharide in the water mold inhibitor is 100-500 mg / L, and the spore concentration of *Epicoccumnigrum* Z01 is 1.0-8.0 × 10⁻⁶ mg / L. 8 / mL. The concentration of Lactobacillus paracasei LY02 in the water mold inhibitor is 1.0-5.0 × 10⁻⁶. 9 CFU / mL. The concentration of ergothioneine in the water mold inhibitor is 300-500 μmol / L. This invention can effectively inhibit water mold infection in aquatic animals.

The invention relates to the technical field of microorganisms, in particular to lactobacillus paracasei ZK-35 and application thereof. The lactobacillus paracasei ZK-35 is separated from a human oral cavity flushing fluid, has good compatibility with the human intestinal environment, high safety and excellent acid and cholate resistance, has an effect of relieving side effects of various antibiotics such as penicillin, cephalosporin and quinolones, can regulate various intestinal florae at the same time, improves constipation and diarrhea, and has a good application prospect in the field of oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid, oral cavity flushing fluid and oral cavity flushing fluid. The intestinal barrier function is enhanced, and nutrient absorption is promoted.

The invention discloses a probiotic which is derived from human milk and has the effects of resisting oxidation, resisting inflammation, protecting tissues and protecting intestinal barriers and application of the probiotic. Lactobacillus paracasei DPUMW-M14-9 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CCTCC M 20252990. The strain has the characteristics of acid resistance, bile resistance, strong oxidation resistance, high surface hydrophobicity, self-aggregation capability and the like. The lactobacillus paracasei DPUMW-M14-9 can effectively adhere to epithelial cells, promote cell proliferation and regulate and control the process of a cell cycle. The bacterial strain can be used for inhibiting proinflammatory cytokines (TNF-alpha, IL-6 and IL-1beta) induced by lipopolysaccharide (LPS) and repairing tight junction proteins (ZO-1, Occludin and Claudin-1) at the same time, so that the integrity of epithelial cells is enhanced.

The present invention refers to a composition including fermented supernatant of Lactobacillus casei or paracasei species. The species is characterized by having in its DNA genome at least one DNA sequence essentially identical to one of the sequences selected from the group consisting of: SEQ ID NO: 1 to 5, and proper diluents and / or carriers and / or excipients. The composition can be used in the treatment and / or prevention of skin and / or dermatological diseases related to inflammation, allergic reactions, contact hypersensitivity reactions and intolerance, excessive sebaceous secretion, exfoliation or microbial dysbiosis, preferably of the epidermis.

The present application belongs to the technical field of natural product extraction and preparation, and particularly relates to a preparation method of garlic polysaccharide and application thereof. The present application finds through experimental research that the garlic polysaccharide product prepared by taking garlic (such as black garlic and fresh garlic) as raw material not only has good antioxidant activity, but also has prebiotic effect and can effectively promote the growth of lactic acid bacteria such as lactobacillus plantarum and lactobacillus casei. In particular, the antioxidant and prebiotic effect of the black garlic polysaccharide product prepared by taking black garlic as raw material is better, so that the garlic polysaccharide product can be widely applied in the fields of food, medicine, daily chemical product and feed, and the comprehensive utilization value of garlic, especially black garlic, is effectively improved, and therefore the present application has wide and good practical application value and prospect.

The invention discloses a composite leavening agent and application of the composite leavening agent in high epiphytic microorganism and high lignocellulose straw silage and biomass pretreatment. The technical problem to be solved is how to improve the quality of silage obtained by fermenting silage raw materials (especially silage raw materials with high epiphytic microorganisms and / or high lignocellulose) and / or shorten the fermentation time of the silage and / or improve the cellulose conversion rate of the silage raw materials. The composite leavening agent provided by the invention is prepared from lactobacillus casei TD, lactobacillus plantarum JT-10, laccase and cellulase. The method can be used for fermentation of silage raw materials with high epiphytic microorganisms and high lignocellulose, the quality, nutritional value and the like of silage can be effectively improved, the conversion efficiency of biomass cellulose can be improved, and biological energy can be produced through pretreatment.

The invention discloses probiotic fermented carrot juice capable of improving intestinal health and resisting oxidation and a preparation method of a metagen. The carrot juice is prepared by fermenting carrot raw materials through a compound probiotic agent containing lactobacillus plantarum YZX21, lactobacillus casei YRL577, lactobacillus paracasei X11 and lactobacillus rhamnosus LR-C111. The preparation method comprises the following steps: cleaning, peeling, crushing and pulping carrots to prepare carrot primary pulp, then carrying out high-temperature instantaneous sterilization treatment on the carrot primary pulp, and carrying out vacuum concentration after sterilization; the concentrated carrot primary pulp is inoculated with the composite probiotic agent, the final inoculation concentration is 102-109 CFU / mL, standing fermentation is conducted for 9-15 hours at the temperature of 30-40 DEG C, the probiotic fermented carrot juice is obtained, and the probiotic fermented carrot juice is subjected to high-temperature sterilization treatment to obtain the postbiotics. The probiotic carrot juice and the metabiotics prepared by the invention have the functions of improving constipation, regulating intestinal flora and improving antioxidant capacity, and the preparation method is stable and efficient.

This invention relates to *Lactobacillus paracasei* NEFU-5 and its applications, belonging to the field of biotechnology. To address the problems of limited functionality and poor direct degradation of metabolites in existing probiotic strains, this invention provides *Lactobacillus paracasei* NEFU-5 (accession number CCTCC NO: M 2026147). This *Lactobacillus paracasei* NEFU-5 can simultaneously and efficiently degrade uric acid, triglycerides, and cholesterol, and possesses excellent probiotic properties and safety. The *Lactobacillus paracasei* NEFU-5 provided by this invention can be used to prepare fermented products and can be used to prevent or improve hyperuricemia, hyperlipidemia, and other concurrent metabolic diseases, showing significant application prospects.

The invention provides a culture medium for lactic acid bacteria after-generation and application, the culture medium takes trehalose as a carbon source and a yeast extract as a nitrogen source, so that the growth biomass of lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus can be remarkably increased, the pH is reduced, and the antibacterial ability is improved. Lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus are prepared through the culture medium for the lactobacillus after-generation, so that the lactobacillus after-generation has a good inhibition effect on main food-borne pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes; the preservative can be widely applied to preservation and preservation of meat products such as chilled chicken and pork. The preparation method of the lactic acid bacteria metagen is simple, convenient, low in cost, natural, safe and suitable for large-scale production.