1719 results about "Physiological saline" patented technology

The invention discloses a tree shrew epilepsy model construction method, which specifically comprises the following steps: (1) preparation of Phrixotoxin-1: dissolving 50 [mu] g of Phrixotoxin-1 in normal saline, fixing the volume of the normal saline to 1 ml, and preparing a solution with the concentration of 50 [mu] g / ml and the pH value of 7.4; and (2) performing intraperitoneal injection on the tree shrew by using the Phrixotoxin-1 solution prepared in the step (1), wherein the injection amount is 4 [mu] g / kg. Tree shrew is a small primate-related animal, has a high brain-body mass ratio, and has a developed nervous system and a stress system similar to human beings, so that the tree shrew model has irreplaceable advantages compared with other non-primate animal models. The excitability of the hippocampus pyramidal neurons of an epilepsy patient is increased due to the weakening of A-type potassium current (IA), and in the hippocampus and cortical neurons, the IA is only generated by a Kv4 potassium channel. According to the invention, a specific peptide inhibitor Phrixotoxin-1 of a Kv4 potassium channel is selected to prepare the tree shrew epilepsy model, and a novel preparation method of the epilepsy model is provided.

The invention belongs to the technical field of medical instruments, and discloses a dynamic pressure adjusting thoracic surgery anti-blocking drainage system which comprises a drainage tube, a drainage bag, a backflow tube, a positioning disc, an electric rotating disc, a blade, an electric vacuum pump and a monitoring mechanism. A drainage tube and a return tube are arranged on the special-shaped tee joint; the tail end of the return pipe is connected with the input end of the electric vacuum pump; the output end of the electric vacuum pump communicates with the drainage bag; a protective shell is arranged on the positioning disc; an electric turntable is arranged on the protective shell, a plurality of blades are arranged at the tail end of a movable rod of the multi-stage medical electric push rod, and a monitoring mechanism is arranged on the protective shell. The internal pressure of the thoracic cavity is monitored in real time, the drainage process is accurately controlled in combination with other physical sign data, and the proper pressure of the thoracic cavity is maintained to improve drainage efficiency; a real-time monitoring and abnormity identification function can timely perceive drainage abnormity; normal saline flushing and mechanical crushing are combined, so that the drainage tube is smooth; the drainage process is comprehensively evaluated in combination with various monitoring data, and potential risks are found as early as possible.

The invention provides an atomizing agent containing stem cell exosomes as well as a preparation method and application of the atomizing agent, and belongs to the technical field of exosomes. Ferroferric oxide magnetic particles are prepared, the surfaces of the ferroferric oxide magnetic particles are modified through tannic acid, berberine and adriamycin are loaded, then the ferroferric oxide magnetic particles and the exosome secreted by adipose-derived mesenchymal stem cells are mixed, incubated and centrifuged, the mixture is added into normal saline, uniform ultrasonic dispersion is conducted, and the atomizing agent containing the stem cell exosome is prepared. The prepared atomizing agent containing the stem cell exosome is simple in preparation method and wide in raw material source, the prepared atomizing agent directly enters the lung through spray administration, the unique performance of magnetic targeting drug delivery is achieved, drugs are released in a targeted and fixed-point mode, the drug effect is improved, and the effects of efficiently promoting tumor cell apoptosis and resisting lung cancer and pneumonia are achieved.

The invention belongs to the technical field of medical sampling, and discloses a gynecological vaginal secretion sampling device which comprises a shell, a sealing cover mechanism, a support mechanism and a sampling mechanism. According to the device, after the shell is taken down, the support mechanism and the sampling mechanism are inserted into the lower body of a patient, then normal saline is injected into the fixing pipe through the liquid injection pipe, and the normal saline expands along with the injection of liquid into the liquid bag, so that an inner plate is pushed to move towards the inner wall of the supporting shell, and then a rubber plate is driven to move outwards; after the rubber plate is in contact with the inner wall of the vagina of the patient, injection of the normal saline is stopped, then the outer cover is rotated to drive the gear to rotate, and the gear drives the rotating rod in the gear to rotate, so that the sampling cotton blocks rotate to dip secretions in the vagina of the patient; by arranging the rotating rods, secretions of different depths of a patient can be sampled through the single rotating rod, secretions of different positions can be sampled through the device due to the arrangement of the multiple rotating rods, and therefore samples are richer.

The invention discloses a tree shrew depression model modeling method which comprises the following steps: (1) dissolving 20 mg of corticosterone in 10 microliters of DMSO (dimethylsulfoxide), and fixing the volume to 1 ml by normal saline; the method comprises the following steps: dissolving 100 mg of estradiol in 1 ml of DMSO (Dimethylsulfoxide) to prepare 100 mg / ml of estradiol stock solution; and (2) carrying out subcutaneous injection on the corticosterone solution prepared in the step (1) to tree shrews at 14: 00-16: 00 in the afternoon every day at the injection amount of 27 mu g / kg, diluting the estradiol stock solution prepared in the step (1) with normal saline after the corticosterone solution is injected for 1 hour, then carrying out subcutaneous injection on the tree shrews at the injection amount of 50-75 mu g / kg, and continuously injecting for 21 days to complete molding. According to the invention, a method of increasing the use dosage of corticosterone and simultaneously adding estradiol (estrogen with the highest in-vivo content and strongest activity) is adopted, so that the modeling time can be shortened. The corticosterone and the estradiol are combined for tree shrew depression modeling, so that the modeling time can be effectively shortened, and the optimal modeling effect is achieved by adopting a special estradiol dosage.

The invention provides a fiber dressing for promoting wound healing and a preparation method and application thereof, and belongs to the technical field of biological materials. The shell of the fiber dressing contains the first medicine for promoting polarization of macrophages, and the first medicine can promote polarization of macrophages at normal and diabetic wound surfaces from M1 type to M2 type; a shell substrate is a mixture of hydrophilic and hydrophobic polymers, so that painless adhesion and stripping of a wound surface can be realized by absorbing seepage and spraying non-irritant normal saline on the wound surface, and secondary injury of the wound surface is reduced; an inner core contains a second medicine and a third medicine which are used for inducing death of M1 type macrophages, so that wound inflammatory response can be reduced, wound bacteria can be killed, wound infection degree can be reduced, and wound healing can be accelerated; the core matrix is a mixture of hydrophilic and hydrophobic polymers with good biocompatibility, can provide mechanical support for the fiber dressing, and provides a slow release effect for the two substances in the core. By controlling the composition of the inner core and the shell, the healing of normal wounds and diabetic wounds can be effectively promoted.

The invention discloses a preparation method of near-infrared response thermoelectric temperature-sensitive supramolecular injectable hydrogel. The preparation method comprises the following steps: dissolving bismuth nitrate pentahydrate and thioacetamide in deionized water to react to obtain Bi2S3 nano-particles, and putting the Bi2S3 nano-particles into a tannic acid TA aqueous solution to react to obtain Bi2S3 coated TA nano-particles; the preparation method comprises the following steps: adding NIPAM, VI, disulfiram and azodiisobutyronitrile into an organic solvent, dissolving to obtain a mixed solution, and heating to obtain a supramolecular temperature-sensitive polymer PNV; sA, TA, PNV and Bi2S3 at TA are dissolved in normal saline, an obtained mixed solution is injected into a mold, a metal salt solution is sprayed, and the thermoelectric temperature-sensitive supramolecular injectable hydrogel is obtained through a reaction. The invention also discloses application of the injectable hydrogel obtained by the preparation method in preparation of products for repairing skin wounds. The injectable hydrogel provided by the invention completes active contraction and thermal-electric stimulation under single near-infrared energy input, and can be used as a photo-thermal platform to relieve bacterial load and promote repair of infected wounds.

The invention discloses a portable wound surface protection brace and an application method thereof, and belongs to the technical field of medical instruments. The brace is of a cylindrical structure, is made of EVA materials, has flexibility, non-toxic characteristics and good supporting performance and is externally wrapped with cotton cloth and provided with ventilation through holes, high-density cotton strips are fixed to the two ends of the inner wall to form a hollow structure, and the circumference of the outer wall can be adjusted through at least three sets of bandages with hook-and-loop fasteners. And an antibacterial coating is added, so that clinical application is expanded. During application, a wound surface is subjected to liquid extraction and hydrogen peroxide cleaning in the earlier stage and then covered with insulin mixed normal saline gauze, and the dressing is prevented from making contact with clothes in cooperation with a brace; and smearing mupirocin ointment in the later stage and performing infrared irradiation, and continuously using the brace to keep the wound dry and zero contact. The brace can be repeatedly used, can adapt to various wound types and patient body types, effectively solves the problems of wound adhesion and friction in traditional nursing, creates a high-oxygen healing environment, remarkably shortens wound healing time, relieves pain of patients and improves life quality, and has good clinical application and popularization value.

The invention provides a non-invasive nerve regulation and control device. The non-invasive nerve regulation and control device comprises an optical fiber, an optical fiber fixing device arranged at one end of the optical fiber, a laser light source arranged at the other end of the optical fiber and a photoelectric conversion material positioning and injecting assembly. The photoelectric conversion material positioning and injecting assembly is used for injecting a photoelectric conversion material into a specified solution injection point; the photoelectric conversion material is powdery and comprises nanoparticles capable of converting near-infrared light into electric signals, and the photoelectric conversion material is dispersed in normal saline so as to be injected into biological tissues; the laser light source irradiates laser in a near-infrared band to an area where a photoelectric conversion material injection point is located, so that the laser is converted into an electric signal through the photoelectric conversion material, and the electric signal stimulates an area where a solution injection point is located. The non-invasive nerve regulation and control device has the advantages of non-invasiveness, high efficiency, system uniqueness, stability and programmable control, can realize accurate regulation and control of neurons and neural circuits, and has a wide application prospect.

The invention relates to the technical field of elastin peptides, in particular to a method for extracting elastin peptides from animal cartilage, which comprises the following specific steps: removing surface connective tissues and fat from animal cartilage, cleaning with normal saline, grinding and degreasing to obtain pretreated cartilage particles; putting the pretreated cartilage particles into a container, adding ionic liquid and stirring; after stirring is completed, centrifugally collecting precipitates; adding the precipitate into a phosphate buffer solution to form a suspension; adding an immobilized compound enzyme, and carrying out an enzymolysis reaction; after the enzymolysis is completed, raising the temperature for inactivation; filtering to obtain enzymatic hydrolysate; adding macroporous resin into the enzymatic hydrolysate, and filtering to obtain clear liquid; and carrying out ultrafiltration on the clear liquid to obtain the elastin peptide. The ionic liquid interacts with impurities in the animal cartilage, so that the impurities are easier to remove, a purer raw material environment is created for subsequent elastin peptide extraction, and the extraction efficiency is improved.

The invention discloses a tree shrew Alzheimer's disease modeling method. The method comprises the following specific steps: mixing an ethanol solution of Microcystin-LR with the concentration of 0.5 [mu] g / [mu] l with an amaninic acid solution, and injecting 2 [mu] l to Meynert basal nuclei on the two sides of a tree shrew respectively; and after drug administration is finished, normally feeding for 2 weeks to finish Alzheimer's disease modeling. The method comprises the following steps: under a sterile condition, sucking 200 microliters of sterile normal saline, adding the sterile normal saline into a small bottle containing 1mg of amanitine, repeatedly blowing and beating to completely dissolve the amanitine, and preparing a solution with the concentration of 5mu g / microliter; the Microcystin-LR solution is a product purchased in the market. According to the invention, a mixed solution of MC-LR (0.1 [mu] g) and IBO solution (3 [mu] g) is respectively injected to Meynert basal nuclei on both sides of the tree shrew, and a stable tree shrew AD model can be established after 2 weeks. After the medicine is administrated for 3 days, tree shrews have AD behavior expressions, such as drooping spirit and reduced activity; after two weeks, the tree shrew brain shows AD typical pathological expressions such as A beta deposition, increase of expression levels of multiple phosphorylation sites of Tau protein and the like.

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The utility model discloses a ureter structure with an air bag, and aims to solve the problems that 0.5 mL of normal saline needs to be injected into the air bag to jack up the air bag to enable the surface of the air bag to be smooth after the normal saline is pumped out when a ureter is pulled out in the prior art, the pain of a patient is prevented from being increased due to the fact that wrinkles of the air bag rub the urethral wall, and the workload is increased due to the increase of operation steps of nurses. And the catheter pulling speed is reduced. The urinary catheter comprises a urinary catheter body, a hose connector is mounted at the left end of the urinary catheter body, an air groove is formed in the outer surface of the urinary catheter body, a positioning air bag is fixedly connected to the inner wall of the air groove, an air channel is formed in the urinary catheter body and communicated with the air groove, and an air bag connector is fixedly connected to the outer surface of the urinary catheter body. Through the design, normal saline in the positioning air bag is quantitatively retained to support the positioning air bag so as to prevent the positioning air bag from wrinkling, the normal saline does not need to be injected into the positioning air bag for the second time, and the workload of medical staff is reduced.

The present invention induces differentiation of mesenchymal stem cells and osteoblast precursor cells into osteoblasts. Provided is a medium for culturing stem cells, the medium including 60-90 vol% basal medium and 10-40 vol% physiological saline, to which 1-100 ng / mL of EGF, 0.2-20.0 ng / mL of FGF-2, 0.2-20.0 ng / mL of PDGF, and 0.5-8.0 mM of magnesium ascorbyl phosphate are further added.

The invention provides oral streptococcus ZRSOR-5 as well as a related product, a method and application thereof. The preservation number of the streptococcus oral cavity ZRSOR-5 provided by the invention in the Guangdong Microbial Culture Collection Center is GDMCC No: 65352, and the preservation number of the streptococcus oral cavity ZRSOR-5 provided by the invention is CGMCC No: 65352. Experiments prove that the SOR preparation is prepared by diluting the SOR preparation with normal saline and inhaling the SOR preparation into mice in advance, so that the survival rate of influenza virus infected mice can be remarkably increased, and lung inflammation and virus load can be reduced. When the ZRSOR-5 preparation provided by the invention is used in advance, pneumonia caused by influenza viruses can be resisted.

The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.

The invention relates to a skin grafting material and a using method thereof. The skin grafting material is composed of compressed sponge and repairing liquid. The compressed sponge is selected from chitosan sponge, collagen sponge or polyvinyl formal sponge; the repairing liquid is selected from normal saline containing human epidermal growth factors and / or normal saline containing superoxide dismutase. The use method comprises the following steps: attaching the skin layer of the cut skin graft to the compressed sponge, then putting the compressed sponge into a medical skin rolling machine, and cutting the skin graft into micro skin graft; the cut miniature skin graft is soaked in the repairing liquid, is attached to the position to be subjected to skin grafting after fully absorbing the liquid and expanding, and is wrapped with gauze. The skin grafting material provided by the invention can reduce damage to newborn granulation when the skin grafting material is removed, has an antibacterial effect and improves scar hyperplasia contracture after a skin grafting wound operation; meanwhile, the sponge which absorbs the liquid and expands can slowly release the repairing liquid, so that skin cell regeneration is promoted, epithelization of a skin grafting area is accelerated, and wound healing is promoted; the healing effect is good, and the application prospect is wide.

The invention provides a formula of a sample eluent. The eluent comprises a surfactant, peptone with a concentration of 9 to 12 g / L, normal saline with a concentration of 7.2 to 9.6 g / L, tween-80 with a concentration of 0.8 to 1.2 g / L, disodium hydrogen phosphate with a concentration of 2.75 to 2.97 g / L and potassium dihydrogen phosphate with a concentration of 1.12 to 1.64 g / L. The eluent can effectively improve the sample recovery rate, and at least 90% of target substances can be successfully recovered after the eluent is treated by specific steps. In the use process, a dry and pollution-free sampling brush head needs to be completely immersed in the prepared eluent, then oscillation is carried out under set conditions to promote a target substance to be dissolved in the liquid, and finally the eluent is collected for subsequent analysis. The method is simple and convenient to operate, high in efficiency and very suitable for extracting and detecting various biological samples.

The invention discloses a disposable balloon catheter with an opening and closing function, which belongs to the technical field of medical instruments and comprises a balloon, a balloon cavity and a catheter. The balloon is arranged on the outer side of the balloon cavity, and a water inlet communicated with the balloon is formed in the side wall of the balloon cavity; one end of the catheter is detachably connected with the balloon cavity and is used for filling water into the balloon; a valve structure for controlling water feeding and discharging is arranged in the balloon cavity; the catheter is of a double-layer structure, and a piston mechanism is arranged at the upper end of the inner-layer catheter and used for abutting against the valve structure to be communicated with the normal saline channel of the balloon. And a fixed balloon body is arranged between the upper end of the outer-layer catheter and the upper end of the inner-layer catheter and is used for butt joint of the catheter and the balloon cavity and conveying normal saline into the balloon. The catheter and the balloon cavity which are detachably connected are arranged, so that the balloon catheter is rapidly placed in a bleeding bronchial lumen through the bronchoscope during treatment, compression or blocking hemostasis is achieved, and discomfort and injury caused by long-time retention of the bronchoscope and the catheter in the airway of a patient are reduced.

The invention relates to the technical field of medical treatment, and discloses an intraperitoneal hepatic vein blood flow blocking device which comprises a drainage tension-brace mechanism, a hepatic vein blocking mechanism is arranged on the surface of the drainage tension-brace mechanism, a catheter recycling mechanism is arranged on the surface of the hepatic vein blocking mechanism, a hydraulic measuring mechanism is arranged at one end of the catheter recycling mechanism, and the hydraulic measuring mechanism is connected with the drainage tension-brace mechanism. And a normal saline injection mechanism is arranged at one end of the hydraulic measuring mechanism. According to the intraperitoneal hepatic vein blood flow blocking device, through the arrangement of the drainage pulling and supporting mechanism, the hepatic vein blocking mechanism, the catheter recycling mechanism, the hydraulic measuring mechanism and the normal saline pushing and injecting mechanism, the hepatic vein can be directly blocked through expansion of the hepatic vein blocking mechanism in the using process, and meanwhile it is guaranteed that the inferior vena cava blood flow can flow through a circulation cavity; therefore, smooth return blood circulation of the inferior vena cava is guaranteed.

The invention belongs to the technical field of bone tissue cryopreservation, and specifically is a method for long-term cryopreservation of autologous bone tissue, comprising the following steps: S1, obtaining a bone tissue sample, washing the bone tissue with physiological saline or phosphate buffer, and removing blood and other residues; S2, preparing a cryopreservation medium, pouring the cryopreservation medium into an inner barrel of a preservation device, wrapping the bone tissue sample with a gauze net bag and hanging it under the middle sealing cover of an outer barrel, and the outer barrel and the inner barrel can slide relative to each other, thereby sinking the bone tissue sample into the cryopreservation medium for preservation; S3, placing the preservation device in a freezing device to pre-freeze the bone tissue; S4, after the pre-freezing is completed, taking out the preservation device and putting it into a liquid nitrogen storage tank to preserve the bone tissue at low temperature; the invention separates the bone tissue and the cryopreservation medium before taking out, so as to reduce the subsequent cleaning workload, reduce the work intensity of medical staff, and improve work efficiency.

The invention discloses a cervical image classification method and system based on wavelet transform multi-modal fusion, and the method comprises the steps: 1) constructing a multi-branch feature coding network, and respectively processing a cervical OCT image, a colposcope normal saline image, an iodine coating image and an acetic acid white image; 2) introducing wavelet transform to perform frequency domain decomposition on the multi-modal image, obtaining high-frequency and low-frequency components and designing a cross-frequency interaction mechanism to more effectively capture the structure and detail information of the image, and extracting each modal feature through a ResNet-18 backbone network; 3) designing a feature fusion module, performing channel stacking on the three colposcope features, compressing the three colposcope features, and cascading the three colposcope features with the OCT features to form fusion features; (4) feature dimension reduction is achieved through double full connection layers, a weighted voting multi-mode decision classification strategy is adopted, a five-fold cross validation strategy is adopted, and a weighted cross entropy loss function is combined to solve the problem of data imbalance, and (5) finally Softmax classification is carried out.

The invention relates to the technical field of analysis and detection, in particular to a substitute matrix for detecting folic acid and 5-methyltetrahydrofolic acid and application of the substitute matrix. The alternative substrate comprises a preservative and a rabbit whole blood diluent; the rabbit whole blood diluent comprises rabbit whole blood and normal saline; the rabbit whole blood accounts for 25%-75% of the volume of the rabbit whole blood diluent. The invention provides a substitute matrix for detecting folic acid and 5-methyltetrahydrofolic acid, which not only can meet the matrix effect requirement, but also can be stably stored for a long time, can be applied to the production and use of folic acid detection reagents or kits, and has important significance on the market-oriented popularization and application of whole blood folic acid detection kits.

The invention discloses an ophthalmologic postoperative atomization nursing device, and relates to the field of medical equipment, the ophthalmologic postoperative atomization nursing device comprises a shell, an air supply unit is arranged on the inner side of the shell, the air supply unit is used for preparing high-pressure air, an air supply box is arranged on one side of the air supply unit, and the high-pressure air prepared by the air supply unit is stored in the air supply box; the device further comprises an atomization unit and an atomization eyeshade unit. According to the ophthalmologic postoperative atomization nursing device, by arranging the three atomizers, medicine with different atomization time can be atomized separately, medicine mixing is avoided, normal saline is placed in one atomizer and used for cleaning remaining atomized medicine mist in the conveying pipe after one medicine is atomized, and the atomization time is shortened; meanwhile, residual atomized medicine is conveyed to the eyes of the patient, medicine waste is avoided, medicine atomization in different atomizers is controlled by driving the special-shaped piston to move in the adjusting pipe through the translation driving piece, and the atomizers do not need to be replaced.

The invention discloses eye drops containing L-ergothioneine and a preparation method of the eye drops. The eye drops are prepared from the following components in parts by weight: 0.0015 to 15 parts of L-ergothioneine, 0.001 to 0.01 part of a bacteriostatic agent, 0.05 to 15 parts of a buffering agent and the balance of normal saline, and the pH value of the eye drops is 5.0 to 7.0. Experiments show that L-ergothioneine can prevent and reverse denaturation of crystalline lens protein, the prepared eye drops containing L-ergothioneine can prevent and reduce opacity of crystalline lens protein, and the prevention and treatment effects on cataract are better than those of existing eye drops.

The invention discloses a meridian and collateral stasis point and stasis degree detection device, and particularly relates to the technical field of meridian detection, the meridian and collateral stasis point and stasis degree detection device comprises a display and a detector, one end of the detector is provided with a containing cavity, and a stable coating assembly is used for stably coating normal saline in the detector on the surface of a human body when the detector moves along the surface of the human body. The stable smearing assembly comprises a smearing ball installed in the containing cavity in a rolling mode and a limiting ring rotationally connected to the interior of the containing cavity, and the smearing ball is of an oval structure and used for smearing normal saline outside the smearing ball to the surface of the human body; through the arrangement of the stable coating assembly, normal saline in the detector can be stably coated on the surface of a human body when the detector moves along the surface of the human body, the situation that a coating ball falls off due to the influence of external force caused by frequent movement of the detector on the human body is avoided, and the coating continuity of the coating ball on the surface of the human body is ensured; and the detection efficiency of the device on human meridian conditions is improved.

The invention discloses a real-time detection method for the activity of fibrinolytic enzyme in whole blood, which comprises the following steps: preparing a fibrinolytic response interference layer film with fibrinolytic enzyme response, and fixing to obtain a microfluidic sample cell; preparing a closed plasmin response and plasmin response interference layer film; analyzing the anti-interference capability of the fibrinolysis response interference layer film on interference protein in whole blood; analyzing the specificity of the fibrinolytic response interference layer film for detecting fibrinolytic enzyme in whole blood; analyzing the sensitivity of the fibrinolytic response interference layer film on the detection of fibrinolytic enzyme in whole blood; diluting the whole blood sample by different multiples with normal saline, introducing the diluted whole blood sample and the diluted whole blood sample with labeled fibrinolytic enzyme into a sample pool formed by the closed fibrinolytic response interference layer film, and detecting the fibrinolytic enzyme activity in the whole blood sample. The method can overcome the interference of colored, complex and viscous whole blood samples, successfully detects the activity of the fibrinolytic enzyme in the whole blood samples, and is stable in detection.

The invention relates to the related technical field of medical instruments, in particular to a contrast injection device for enhancing nuclear magnetic resonance, which comprises a base, a mounting shell arranged at the upper part of the base, a first barrel arranged at the left upper part in the mounting shell, a second barrel arranged at the right upper part in the mounting shell, and a mixing barrel arranged in the middle of the upper part of the mounting shell, a first piston rod is arranged in the first barrel, a second piston rod is arranged in the second barrel, communicating pipes are respectively communicated between the first barrel and the mixing barrel and between the second barrel and the mixing barrel, a one-way water inlet pipe is arranged at the top of the barrel, and the one-way water inlet pipe is communicated with an external liquid storage tank. By rotating the adjusting screw rod, the adjusting screw rod drives the first wedge-shaped block to move to the position of the needed dosage, then the rodless air cylinder controls the extrusion plate to move upwards, and after the extrusion plate moves upwards and makes contact with the plate body, the piston rods move upwards to inject the contrast agent and normal saline of the corresponding dosage into the mixing barrel to be mixed.

The invention relates to a heparinized gelatin / sodium alginate pancreas islet packaging material with an anti-fibrosis function as well as a preparation method and application of the heparinized gelatin / sodium alginate pancreas islet packaging material. The preparation method comprises the following steps: adding gelatin into normal saline, soaking, performing autoclaved sterilization to obtain a gelatin solution, then adding epsilon-polylysine hydrochloride into the gelatin solution, and finally adding glutamine transaminase for reaction to obtain an epsilon-polylysine grafted gelatin solution; adding heparin into the solution, and reacting to obtain a heparinized gelatin solution; adding sodium alginate and glutamine transaminase, reacting, diluting, preparing hydrogel microspheres by adopting an electrostatic spraying method, and cross-linking to obtain the hydrogel microspheres. According to the invention, heparin molecules are incorporated into a pancreas islet packaging system, so that fibrosis at the periphery of the microspheres can be relieved, the pancreas islet cells are packaged by using the pancreas islet packaging material to prepare the biological artificial pancreas, the pancreas islet cell proliferation and insulin secretion can be promoted, and the pancreas islet packaging material has a good diabetes treatment effect.

The invention relates to the technical field of medical auxiliary instruments, and particularly discloses an infusion port pressure sore prevention fixing device which comprises an injection fixing cover, a normal saline arc storage frame is fixedly connected to the outer side of the bottom end of the injection fixing cover, and a pressure sore prevention nursing arc frame is fixedly connected to the outer end of the normal saline arc storage frame; after the infusion port injection fixing cover is installed stably, a butt joint pressing plate is pressed to enable a lifting conduction arc plate to move downwards in a pressure sore prevention nursing arc frame, and normal saline in a normal saline arc storage frame flows into a hook-and-loop fastener cleaning and wiping cotton cushion through a normal saline seepage hole to be absorbed by the hook-and-loop fastener cleaning and wiping cotton cushion; the hook-and-loop fastener cleaning and wiping cotton pad cleans the skin outside the infusion port implantation position of a patient, the pressure sore risk caused by long-time pressing of the skin is reduced, the hook-and-loop fastener cleaning and wiping cotton pad wipes normal saline on the skin of the affected part to reduce friction and stimulation to the skin, the comfort of the patient is improved, meanwhile, the occurrence rate of pressure sores is further reduced, and the use safety of the patient is improved. The risk of cross infection is reduced by automatic delivery of normal saline.