The invention relates to the technical field of interventional
medicine, in particular to sulfonated
gelatin microspheres and a preparation method thereof. The preparation method comprises the following steps: adding
gelatin into a
buffer solution, heating, stirring and dissolving to obtain a water phase, adding a dispersing agent into an
oil phase matrix solution, stirring to obtain an
oil phase, transferring the water phase into the
oil phase, stirring, and cooling to obtain gel microspheres; the preparation method comprises the following steps: primarily cleaning gel microspheres, putting the gel microspheres into a freezing medium for
freeze drying, then adding the freeze-dried gel microspheres into normal
saline, adding a cross-linking agent for a cross-linking reaction, then adding a sulfonation
reagent, a catalyst and an initiator for a sulfonation reaction, and finally, carrying out secondary cleaning on the microspheres after the sulfonation reaction is finished, so as to obtain the hydrogel. The sulfonated
gelatin microspheres are obtained. According to the preparation method, the cross-linked microspheres are firstly prepared, then the cross-linked microspheres are subjected to sulfonation treatment, the
reaction conditions are mild, the process is simple, amplified production is easier to perform, the microspheres (45 [mu] m) with very
small particle sizes can be obtained, and the adhesion condition is avoided.