15 results about "Lipid formation" patented technology

The invention relates to the technical field of circulating water systems, in particular to a circulating water system protective agent and a preparation method thereof.The circulating water system protective agent is prepared from, by weight, 20-40 parts of lipid matrixes, 10-20 parts of carboxyl-containing organic compounds, 8-15 parts of ester-containing organic compounds, 5-12 parts of hydrophilic functional auxiliaries, 5-8 parts of modified functional agents, 4-7 parts of nano silver powder, 1-3 parts of dispersing agents and 4-7 parts of auxiliary agents. The polyol ester in the formula can form a stable complex with calcium and magnesium ions in water, and crystal nucleus formation and growth of carbonate and sulfate scales are inhibited; the carboxyl-containing organic compounds (adipic acid and sebacic acid) have strong chelating ability, and can disperse scale forming ions in water and prevent deposition of scale bodies.

The invention discloses a universal sample type nucleic acid releasing agent and application thereof. The nucleic acid releasing agent is prepared from the following components in molar concentration: 20 to 100 mM of Tris-HCl, 30 to 500 mM of sodium chloride, 30 to 200 mM of ammonium sulfate, 1 to 6 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 8 mM of a reducing agent, 0.1 to 0.2 percent of sodium dodecyl sarcosinate, 0.1 to 3 mM of Surfactin, 5 to 30 percent of Chelex-100 and 5 to 15 percent of trehalose. According to the method, various samples can be mildly split, impurities such as impure proteins and lipids can be effectively removed, the complexity of nucleic acid samples is reduced, and the method is seamlessly jointed with subsequent PCR (Polymerase Chain Reaction); the kit has double effects of sample preservation and splitting decomposition, can strongly inhibit various nuclease, and can ensure the long-term storage stability of samples.

This invention relates to the field of pharmaceutical manufacturing, specifically to a method for preparing naturally sourced injectable cholesterol. The method includes the following steps: taking egg yolk oil, allowing it to settle, centrifuging the lower sediment at high speed, adding an organic solvent to the centrifuged solid for low-temperature extraction to remove residual glycerol lipids, taking the filtered extract, adding a first crystallization solvent for recrystallization and purification twice, taking the crude cholesterol product after recrystallization, adding a second crystallization solvent for further recrystallization and purification, and drying under reduced pressure to obtain the injectable cholesterol product. This invention solves the technical problem of preparing injectable cholesterol from egg yolk, is suitable for large-scale industrialization of cholesterol, and can be applied to the preparation of complex formulations such as liposomes, improving the safety of drug use.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The application belongs to the field of pharmaceutical products, and particularly relates to a squalene modified polyphenol-chlorophyll flexible nanoliposome as well as a preparation method and application thereof. An organic phase containing phospholipid substances, squalene and the like is prepared, a lipid film is obtained through rotary evaporation and vacuum drying, and the lipid film is hydrated with a polyphenol buffer, ultrasonicated and extruded through a filter membrane to obtain the nanoliposome. The nanoliposome promotes scarless wound healing through three mechanisms of antioxidation, antibiosis and anti-fibrosis, has skin affinity and super-flexible penetration properties, and has excellent stability and transdermal properties, and can be used for preparing wound repair and drug delivery drugs, and has a wide application prospect.

The present application relates to a kind of lipid droplet targeted AIE molecule in seaweed dyeing imaging and screening etc., the AIE molecule is a kind of aldehyde group substitution triphenylamine skeleton aggregation-induced emission organic fluorescent molecule, it has bright blue-green fluorescence, good biocompatibility, excellent seaweed lipid droplet targeting nature.Compared with commercial probe Nile red, it has better specific marking effect. After using this probe to dye seaweed, the size, content and change of lipid droplet in seaweed can be clearly observed.Combined with flow cytometry screening technology, seaweed with strong fluorescent signal is screened, and seaweed with more and larger lipid droplets is obtained, and the culture result shows that the yield of seaweed biological lipids and the proportion of lipid material in dry weight are significantly improved, about twice of the original culture before screening.Therefore, it has important significance and value in marine microalgae research and biological crude oil production etc.

The application discloses a combined metabolic marker and detection kit for mitochondrial purity evaluation, the marker comprises phosphatidylcholine PC 34:0 (16:0 / 18:0), sphingomyelin SM 34:1;2O (d18:1;2O / 16:0), sphingomyelin SM 44:2;2O (18:1;2O / 24:1) and hexosylceramide HexCer 42:2;2O (d18:1;2O / 24:1), and is used for judging the new application and kit of mitochondrial purity of mitochondria extracted by a mitochondrial extraction method. The application judges the mitochondrial purity extracted by the method by detecting the relative concentrations of four lipid metabolites in the mitochondrial sample, calculating a combined marker variable P based on a binary logistic regression equation and determining a cut-off value. The kit can realize high-sensitivity and high-efficiency detection of the four small molecule metabolites, and has the characteristics of low detection cost and good repeatability.

This invention belongs to the field of food raw material science and discloses a chitosan-dextran nanopolysaccharide complex, its preparation method, and its applications. This invention proposes the use of an emulsifier. The combination of a low-concentration alkali and the emulsifier, along with a shortened processing time, preserves the natural properties of the complex while maximally removing related compounds such as proteins, lipids, fats, pigments, alkali-soluble dextran, and mineral compounds. This improves product purity while reducing CGC degradation during preparation and increasing removal efficiency. The chitosan-dextran nanopolysaccharide complex of this invention, due to its nano-sizing, provides optimal dispersibility in a hydrophilic environment, promoting better entry into the human body and interaction with cell surface receptors. It regulates the content of giant cells and the activity of T lymphocytes in vivo, effectively improving human immune regulation, and primarily inhibits tumors by enhancing the body's non-specific immune function.

This application discloses an emulsion composition, an emulsion, its preparation method, uses, and cosmetics, belonging to the field of chemical production technology. The technical solution is as follows: an emulsion composition containing at least two of Bacillus ferment, glycolipid emulsifiers, and phospholipid emulsifiers, and containing at least Bacillus ferment. This emulsion composition uses Bacillus ferment as the main emulsifier, combined with other biological emulsifiers, to enhance the application range and stability of the D-phase emulsion system. Furthermore, this application has also found through testing that when the D-phase emulsion system is used in conjunction with a special oil phase, the brightening and soothing abilities of the emulsion can be further enhanced.

The invention relates to the technical field of microorganisms, in particular to edwardsiella bacteriophage as well as a composition and application thereof. The Edwardsiella phage is an Edwardsiella phage EDP3AEDQD6 (Edwardiella phage EDP3AEDQD6), the Edwardsiella phage EDP3AEDQD6 is preserved in the Guangdong Microbial Culture Collection Center on August 18, 2025, and the preservation number is GDMCC NO. 66852-B1. The invention further discloses a preparation method of the Edwardsiella phage. The bacteriophage has a relatively broad-spectrum host range, and can still keep relatively high activity while splitting target bacteria; meanwhile, the bacteriophage is excellent in alkali resistance and thermal stability, can adapt to the application requirements of multiple temperature nodes in production, does not contain lipid substances in the capsid, and has a good application prospect.

The invention relates to the field of chemical analysis, and discloses a liquid chromatographic column for separating glycol ester isomeride as well as application and an application method of the liquid chromatographic column, and chromatographic column filler comprises amino resin modified silicon dioxide microspheres. The liquid chromatographic column can effectively separate the isomers of the diol esters, the purity of the two isomers of the separated diol esters exceeds 99%, the preparation raw materials are simple in component, easy to obtain and low in cost, the preparation process is simple, no environmental pollution is caused, and the consistency of the chromatographic column is high.

The invention relates to the field of printing ink cleaning agents, in particular to a composite system water-based ink cleaning agent and a preparation method thereof, and solves the problems that an existing water-based ink cleaning agent is relatively weak in permeability to stubborn ink stains, and a composite enzyme system aiming at lipid substances is easily influenced by a strong surfactant. The preparation method comprises the following steps: activating mesoporous silicon dioxide nanoparticles in a nitric acid solution, reacting the activated mesoporous silicon dioxide nanoparticles with 3-glycidoxypropyltrimethoxysilane to obtain epoxy powder, carrying out glycerolation on the epoxy powder, and reacting the glycerolated epoxy powder with glutaraldehyde to obtain an activated carrier; and finally, mixing the biological enzyme with the activated carrier to form immobilized enzyme particles, and uniformly mixing the immobilized enzyme particles with various assistants to obtain the composite system water-based ink cleaning agent. Unification of high activity of the enzyme body and high permeability of the cleaning agent can be realized, so that damage of a surfactant to the enzyme body is reduced, and finally, the capability of removing printing ink stains is improved.

The application relates to a class of lipid molecules containing photosensitizers and an application thereof, and belongs to the fine chemical field. The series of molecules are connected with photosensitizers with a photodynamic treatment effect and lipid molecules, belong to a class of novel bifunctional material molecules. The molecules contain lipid side chains, have the performance of combining RNA / DNA and other base sequences and self-assembling into nanoparticles, become high-quality RNA / DNA delivery carrier materials; and the photosensitizer part in the molecules can generate singlet oxygen to induce tumor cell death under light source irradiation. The series of molecules have the RNA / DNA delivery performance and the ability of photodynamic damage to tumor cells, can efficiently connect gene therapy and photodynamic therapy.

A lipid nanoparticle, and a preparation method therefor and the use thereof. The lipid nanoparticle comprises packaging materials, namely, cationic lipids, neutral lipids, PEG or PEGylated lipids, and cholesterol or derivatives thereof at a molar percentage of (33-50)%:(10-13.33)%:(1.5-2)%:(38.5-51.33)%. The synthesis of a targeted LNP within the molar percentage range has a higher encapsulation efficiency, and can significantly improve the transduction efficiency of the targeted LNP, which can not only improve the encapsulation efficiency and loading capacity of the particle, but also enhances the targeting function of T cells, thereby realizing highly efficient transfection of T cells.