35 results about "Lipid formation" patented technology

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention relates to the technical field of circulating water systems, in particular to a circulating water system protective agent and a preparation method thereof.The circulating water system protective agent is prepared from, by weight, 20-40 parts of lipid matrixes, 10-20 parts of carboxyl-containing organic compounds, 8-15 parts of ester-containing organic compounds, 5-12 parts of hydrophilic functional auxiliaries, 5-8 parts of modified functional agents, 4-7 parts of nano silver powder, 1-3 parts of dispersing agents and 4-7 parts of auxiliary agents. The polyol ester in the formula can form a stable complex with calcium and magnesium ions in water, and crystal nucleus formation and growth of carbonate and sulfate scales are inhibited; the carboxyl-containing organic compounds (adipic acid and sebacic acid) have strong chelating ability, and can disperse scale forming ions in water and prevent deposition of scale bodies.

The invention discloses a universal sample type nucleic acid releasing agent and application thereof. The nucleic acid releasing agent is prepared from the following components in molar concentration: 20 to 100 mM of Tris-HCl, 30 to 500 mM of sodium chloride, 30 to 200 mM of ammonium sulfate, 1 to 6 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 8 mM of a reducing agent, 0.1 to 0.2 percent of sodium dodecyl sarcosinate, 0.1 to 3 mM of Surfactin, 5 to 30 percent of Chelex-100 and 5 to 15 percent of trehalose. According to the method, various samples can be mildly split, impurities such as impure proteins and lipids can be effectively removed, the complexity of nucleic acid samples is reduced, and the method is seamlessly jointed with subsequent PCR (Polymerase Chain Reaction); the kit has double effects of sample preservation and splitting decomposition, can strongly inhibit various nuclease, and can ensure the long-term storage stability of samples.

The present invention relates to the technical field of additive stirring, and specifically to a lipid additive stirring device for feed coating, which includes a base, and also includes: a material containing and discharging structure connected to the base, the material containing and discharging structure includes a first rotation driving part, the first rotation driving part is connected with a connecting cover, the connecting cover is connected with a sampling mechanism, a baffle is slidably installed below each group of connecting covers, the baffle is connected with a bracket, a closing plate is slidably connected to the bottommost group of baffles, and the lower end surfaces of the remaining four groups of baffles are respectively slidably connected to the upper end surfaces of the four groups of connecting covers, and the baffle is connected with a second rotation driving part; a lifting and stirring structure connected to the base. During the process of stirring the material, the present invention is provided with a sampling mechanism to assist personnel in detecting the stirring and processing conditions of the material at different heights, and by adjusting the material containing and discharging structure and the lifting and stirring structure, a layer of the stirred material is separately separated and taken out, avoiding over-stirring of the material and improving the processing efficiency.

This invention relates to the field of pharmaceutical manufacturing, specifically to a method for preparing naturally sourced injectable cholesterol. The method includes the following steps: taking egg yolk oil, allowing it to settle, centrifuging the lower sediment at high speed, adding an organic solvent to the centrifuged solid for low-temperature extraction to remove residual glycerol lipids, taking the filtered extract, adding a first crystallization solvent for recrystallization and purification twice, taking the crude cholesterol product after recrystallization, adding a second crystallization solvent for further recrystallization and purification, and drying under reduced pressure to obtain the injectable cholesterol product. This invention solves the technical problem of preparing injectable cholesterol from egg yolk, is suitable for large-scale industrialization of cholesterol, and can be applied to the preparation of complex formulations such as liposomes, improving the safety of drug use.

The invention provides lipid rich in sphingomyelin, a preparation method and a phospholipid ingredient for infant formula milk powder, and relates to the technical field of dairy product processing.The lipid rich in sphingomyelin and the preparation method thereof have the advantages that primary separation is carried out by the aid of supercritical carbon dioxide extraction technologies, secondary extraction is carried out by the aid of follow-up ethyl alcohol serving as entrainer, non-polar lipid can be effectively removed, and the lipid rich in sphingomyelin can be obtained; and phospholipids with high polarity, especially sphingomyelin, are retained. The ethanol concentration is controlled to be 20% (v / v), so that the solubility and the selective extraction efficiency of lipid rich in sphingomyelin are improved, and a higher sphingomyelin enrichment degree is realized in a final product. Meanwhile, ethanol extraction can be directly combined with a freezing crystallization method, other organic solvents are not introduced into the method, the method is green and safe, complex equipment support is not needed, the technological process is short, operation is easy and convenient, cost is low, and the method is suitable for rapid preparation.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The invention discloses a plant polysaccharide composite film-forming agent as well as a preparation method and application thereof, and belongs to the technical field of natural film-forming agents. The film-forming agent disclosed by the invention is prepared from the following components in parts by weight: 30 to 45 parts of garlic polysaccharide, 10 to 20 parts of oat beta-glucan, 5 to 15 parts of collagen, 3 to 10 parts of sericin and 40 to 45 parts of glycerol, and the prepared composite film-forming agent has the antibacterial property of polysaccharide, the long-chain polymer structure of protein and the hydrophobicity of lipid substances; a thin film with certain gas barrier property and moisture barrier property can be formed, the strength and the adhesion property of the composite film are enhanced, and the composite film can be applied to facial masks, medical use or fresh keeping.

The application belongs to the field of pharmaceutical products, and particularly relates to a squalene modified polyphenol-chlorophyll flexible nanoliposome as well as a preparation method and application thereof. An organic phase containing phospholipid substances, squalene and the like is prepared, a lipid film is obtained through rotary evaporation and vacuum drying, and the lipid film is hydrated with a polyphenol buffer, ultrasonicated and extruded through a filter membrane to obtain the nanoliposome. The nanoliposome promotes scarless wound healing through three mechanisms of antioxidation, antibiosis and anti-fibrosis, has skin affinity and super-flexible penetration properties, and has excellent stability and transdermal properties, and can be used for preparing wound repair and drug delivery drugs, and has a wide application prospect.

This application relates to a decellularized scaffold for cartilage repair in the biomedical field, its preparation method, and application. The preparation method comprises: preparing a decellularized matrix from animal cartilage; chemically bonding the decellularized matrix with a lipid compound to obtain a double-bond-modified decellularized matrix; copolymerizing and cross-linking the double-bond-modified decellularized matrix to obtain a decellularized matrix hydrogel; and immersing the decellularized matrix hydrogel in a dipeptide solution for modification to obtain the decellularized scaffold. The decellularized scaffold of this application introduces both mechanical and electrical stimulation effects into the scaffold, addressing deficiencies in previous research and demonstrating good biocompatibility and repair effectiveness in both in vivo and in vitro experiments.

The application discloses a method for selectively separating bisphenol compounds in serum, and belongs to the technical field of extraction and purification of organic pollutants. The method is suitable for analyzing bisphenol compounds such as BPA, BPS, BPF, BPE, BPB, BPAF, BPAP, BPZ and TBBPA in serum. Zeolite molecular sieves are filled into a solid-phase extraction empty tube, and the filled solid-phase extraction column is activated by using an organic solvent to remove possible impurities. Then, serum extract is loaded onto the solid-phase extraction column. A certain volume of dichloromethane is used for elution to remove part of interfering substances. Then, a mixed solvent of methanol / dichloromethane with a proper ratio is used for elution to collect target substances. According to the application, some lipid macromolecules in serum can be removed through zeolite molecular sieve solid-phase extraction, which greatly reduces the matrix effect of serum, guarantees the recovery rate of bisphenol compounds, saves cost and is more environmentally friendly.

The present application relates to a kind of lipid droplet targeted AIE molecule in seaweed dyeing imaging and screening etc., the AIE molecule is a kind of aldehyde group substitution triphenylamine skeleton aggregation-induced emission organic fluorescent molecule, it has bright blue-green fluorescence, good biocompatibility, excellent seaweed lipid droplet targeting nature.Compared with commercial probe Nile red, it has better specific marking effect. After using this probe to dye seaweed, the size, content and change of lipid droplet in seaweed can be clearly observed.Combined with flow cytometry screening technology, seaweed with strong fluorescent signal is screened, and seaweed with more and larger lipid droplets is obtained, and the culture result shows that the yield of seaweed biological lipids and the proportion of lipid material in dry weight are significantly improved, about twice of the original culture before screening.Therefore, it has important significance and value in marine microalgae research and biological crude oil production etc.

The invention provides a fluticasone furoate micellar solution. The fluticasone furoate micellar solution comprises fluticasone furoate, a lipophilic component and a nonionic surfactant. According to the fluticasone furoate pharmaceutical composition, the dissolution rate and the absorption rate of fluticasone furoate on nasal mucosa and alveolar mucosa can be remarkably improved, and the administration dosage can be further reduced while the same curative effect is obtained, so that the toxic and side effects are reduced. The aerosol inhalation powder is especially suitable for aerosol inhalation, can provide better pulmonary deposition, and is especially suitable for nasal administration to reduce the administration dosage and improve the bioavailability.

The application discloses a combined metabolic marker and detection kit for mitochondrial purity evaluation, the marker comprises phosphatidylcholine PC 34:0 (16:0 / 18:0), sphingomyelin SM 34:1;2O (d18:1;2O / 16:0), sphingomyelin SM 44:2;2O (18:1;2O / 24:1) and hexosylceramide HexCer 42:2;2O (d18:1;2O / 24:1), and is used for judging the new application and kit of mitochondrial purity of mitochondria extracted by a mitochondrial extraction method. The application judges the mitochondrial purity extracted by the method by detecting the relative concentrations of four lipid metabolites in the mitochondrial sample, calculating a combined marker variable P based on a binary logistic regression equation and determining a cut-off value. The kit can realize high-sensitivity and high-efficiency detection of the four small molecule metabolites, and has the characteristics of low detection cost and good repeatability.

The invention belongs to the technical field of reverse osmosis, and particularly relates to a repair additive suitable for an oxidized reverse osmosis membrane and a preparation method of the repair additive. The repairing additive comprises the following components in percentage by mass: 5%-10% of a novel biological degradable material, 80%-89.95% of a lipid organic compound, 5%-10% of an organic solvent and 0.05%-0.1% of a thickening agent, according to the method, the desalination rate of the oxidized reverse osmosis membrane can be recovered to 97% or above, the recovery rate of a single membrane can be recovered to about 15%, and the repair effect can be stably maintained for half a year or above; and meanwhile, the reverse osmosis water production can be maintained not to be reduced.

An automatic extraction device for large-volume free nucleic acid through a centrifugal column method comprises a back plate, a shifting mechanism and a reagent filling module, the shifting mechanism and the reagent filling module are sequentially and fixedly arranged on the back plate, and a centrifugal column filtering module with a negative-pressure liquid pumping module is arranged at the bottom of the reagent filling module. Through a centrifugal column specifically combined with nucleic acid and a special reagent filling module, after a sample is subjected to cracking and protease K digestion treatment, nucleic acid in the sample is rapidly adsorbed and rapidly washed through a filter membrane, so that protein, pigments, lipids and other impurities are removed to the maximum extent; the obtained total nucleic acid can be stored in an eluent for a long time to be used for a next experiment. The whole device can realize centrifugal column method large-volume free nucleic acid automatic extraction for extracting, enriching and purifying the free total nucleic acid from fresh or frozen serum, plasma, lymph and other cell-free body fluids.

This invention belongs to the field of food raw material science and discloses a chitosan-dextran nanopolysaccharide complex, its preparation method, and its applications. This invention proposes the use of an emulsifier. The combination of a low-concentration alkali and the emulsifier, along with a shortened processing time, preserves the natural properties of the complex while maximally removing related compounds such as proteins, lipids, fats, pigments, alkali-soluble dextran, and mineral compounds. This improves product purity while reducing CGC degradation during preparation and increasing removal efficiency. The chitosan-dextran nanopolysaccharide complex of this invention, due to its nano-sizing, provides optimal dispersibility in a hydrophilic environment, promoting better entry into the human body and interaction with cell surface receptors. It regulates the content of giant cells and the activity of T lymphocytes in vivo, effectively improving human immune regulation, and primarily inhibits tumors by enhancing the body's non-specific immune function.

The invention belongs to the technical field of lipophilic adsorption materials, and particularly relates to a lipophilic adsorption material, a preparation method thereof and an aerosol generating device. The preparation method of the lipophilic adsorption material comprises the steps that a lipophilic and hydrophobic first adsorption material is prepared, the first adsorption material comprises a three-dimensional porous base material, an oxide micro-nano structure array is vertically distributed on the surface of the three-dimensional porous base material, and the surface of the three-dimensional porous base material is modified with a lipophilic and hydrophobic layer; preparing a lipophilic and hydrophilic second adsorption material; and combining the first adsorption material and the second adsorption material to obtain the lipophilic adsorption material. Through the combined action of the first adsorption material and the second adsorption material, the adsorption efficiency on lipid molecules and condensate is high, the lipid molecules and the condensate can be rapidly fixed, and the problem of solvent leakage is avoided. Moreover, the lipophilic adsorption material is large in adsorption capacity, so that the containing capacity of lipid molecules and condensate is improved, and the service life of the lipophilic adsorption material is prolonged.

This application discloses an emulsion composition, an emulsion, its preparation method, uses, and cosmetics, belonging to the field of chemical production technology. The technical solution is as follows: an emulsion composition containing at least two of Bacillus ferment, glycolipid emulsifiers, and phospholipid emulsifiers, and containing at least Bacillus ferment. This emulsion composition uses Bacillus ferment as the main emulsifier, combined with other biological emulsifiers, to enhance the application range and stability of the D-phase emulsion system. Furthermore, this application has also found through testing that when the D-phase emulsion system is used in conjunction with a special oil phase, the brightening and soothing abilities of the emulsion can be further enhanced.

The invention discloses photosensitive gel with eucalyptus oil microemulsion wrapped by a biomimetic membrane as well as a preparation method and application of the photosensitive gel. The preparation method comprises the following steps: taking eucalyptus oil and a lipid compound as a mixed oil phase, wrapping the outer layer of eucalyptus oil microemulsion with a biomimetic membrane, and then distributing the eucalyptus oil microemulsion wrapped with the biomimetic membrane in a photosensitive gel matrix of dopamine modified methacrylic anhydride gelatin to prepare the photosensitive gel with the eucalyptus oil microemulsion wrapped with the biomimetic membrane. The photosensitive gel has high adhesion and capability of actively targeting periodontitis pathogenic bacteria, and the treatment effect on periodontitis is remarkably improved.

The invention relates to the technical field of microorganisms, in particular to edwardsiella bacteriophage as well as a composition and application thereof. The Edwardsiella phage is an Edwardsiella phage EDP3AEDQD6 (Edwardiella phage EDP3AEDQD6), the Edwardsiella phage EDP3AEDQD6 is preserved in the Guangdong Microbial Culture Collection Center on August 18, 2025, and the preservation number is GDMCC NO. 66852-B1. The invention further discloses a preparation method of the Edwardsiella phage. The bacteriophage has a relatively broad-spectrum host range, and can still keep relatively high activity while splitting target bacteria; meanwhile, the bacteriophage is excellent in alkali resistance and thermal stability, can adapt to the application requirements of multiple temperature nodes in production, does not contain lipid substances in the capsid, and has a good application prospect.

The invention relates to the field of mass spectrometry, and particularly provides a method for determining a lipid chemical structure and an ion mobility spectrometry tandem mass spectrometer. The method for determining the lipid chemical structure comprises the following steps: an ionization step: ionizing a sample to obtain sample ions; an ion mobility separation step: separating target lipid ions from the sample ions based on the ion mobility; a first dissociation step: dissociating the target lipid ions by dissociation energy suitable for breaking a first chemical bond of the target lipid ions; a mass selection step: based on the mass number, selecting a target lipid ion with the first chemical bond broken to obtain a fragment ion; a second dissociation step: dissociating the fragment ions, at least breaking a second chemical bond of the fragment ions, the bond energy of which is higher than that of the first chemical bond, to obtain diagnosis ions; and a mass analysis step: performing mass analysis on the diagnosis ions.

The invention discloses a chromatographic detection method and device for residual pesticides and chemical substances in food in the technical field of food detection, and the method comprises the following steps: step 1, crushing the food to be detected, sequentially adding purified water and lipid, and mixing with the crushed food; 2, carrying out water bath heating on the crude mixture, and filtering by using a filter screen to obtain a turbid liquid; step 3, pouring the turbid liquid into a separation pipe, carrying out centrifugal separation, and carrying out condensation treatment; 4, after condensation treatment is completed, supernate in the dissolving solution is extracted, and freezing treatment is conducted; separating the solidified lipid layer on the central tube from the separation tube; step 5, putting the frozen supernatant into a standard solution for chromatographic detection, and putting a solidified block on the uppermost layer of the lipid layer into the standard solution for chromatographic detection to obtain a chromatographic detection report; therefore, the food sample can be quickly purified, and the detection time is shortened.

The present invention belongs to the field of biological preparations, and specifically relates to a nanocomposite emulsion of dihydromyricetin, a preparation method thereof, and an application thereof. The nanocomposite emulsion of dihydromyricetin proposed by the present invention comprises the following components, calculated by weight percentage: 0.5-3.0 wt.% dihydromyricetin, 2.0-8.0 wt.% hydrophilic core solvent, 12.5-25.5 wt.% lipid carrier with a bidirectional hydrophilic structure, and 63.5-85.0 wt.% external aqueous phase. The present invention first uses a hydrophilic core solvent to completely dissolve DHM. The invention overcomes the defects of the prior art, greatly improves the solubility of DHM, and significantly increases its room temperature stability. The DHM lipid particles can reach the nanometer level and are not easily segregated, which significantly improves the permeability of DHM and enhances its bioavailability.

The invention relates to the field of chemical analysis, and discloses a liquid chromatographic column for separating glycol ester isomeride as well as application and an application method of the liquid chromatographic column, and chromatographic column filler comprises amino resin modified silicon dioxide microspheres. The liquid chromatographic column can effectively separate the isomers of the diol esters, the purity of the two isomers of the separated diol esters exceeds 99%, the preparation raw materials are simple in component, easy to obtain and low in cost, the preparation process is simple, no environmental pollution is caused, and the consistency of the chromatographic column is high.

The present invention provides a degradable multi-functional nanoemulsion, a preparation method thereof and an application thereof. Based on 100% of the total weight of the nanoemulsion, it comprises: 5-15% of a first surfactant, 5-15% of a second surfactant, 10-30% of a co-surfactant, 5-20% of an oil phase and 30-75% of water; wherein, the first surfactant comprises a glycolipid biosurfactant; the second surfactant comprises one or a combination of several of dodecyl glucoside, alkanolamide, fatty acid methyl ester sulfonate and fatty amide alkyl betaine. The present invention also provides an application of the nanoemulsion in a fracturing fluid and / or an oil displacement agent. The nanoemulsion of the present invention can effectively reduce the viscosity of crude oil and enhance the imbibition effect of a heavy oil reservoir, and has the advantages of being completely biodegradable, having low reservoir damage, being safe and non-toxic, having a simple preparation, and having a low recovery and treatment cost, etc.

The invention relates to the field of printing ink cleaning agents, in particular to a composite system water-based ink cleaning agent and a preparation method thereof, and solves the problems that an existing water-based ink cleaning agent is relatively weak in permeability to stubborn ink stains, and a composite enzyme system aiming at lipid substances is easily influenced by a strong surfactant. The preparation method comprises the following steps: activating mesoporous silicon dioxide nanoparticles in a nitric acid solution, reacting the activated mesoporous silicon dioxide nanoparticles with 3-glycidoxypropyltrimethoxysilane to obtain epoxy powder, carrying out glycerolation on the epoxy powder, and reacting the glycerolated epoxy powder with glutaraldehyde to obtain an activated carrier; and finally, mixing the biological enzyme with the activated carrier to form immobilized enzyme particles, and uniformly mixing the immobilized enzyme particles with various assistants to obtain the composite system water-based ink cleaning agent. Unification of high activity of the enzyme body and high permeability of the cleaning agent can be realized, so that damage of a surfactant to the enzyme body is reduced, and finally, the capability of removing printing ink stains is improved.

The invention provides earthworm protein concentration and purification equipment and method, and belongs to the technical field of purification equipment. Comprising a tower, a support and a main tank arranged on the tower, an inlet pipe is arranged at the top end of the main tank, an inner centrifugal cylinder, a chromatographic column assembly and a driving mechanism for driving the inner centrifugal cylinder are arranged on the support, the inner centrifugal cylinder is connected with the inlet pipe, the inlet pipe is connected with the chromatographic column assembly, and the inner centrifugal cylinder is used for centrifugally layering mixed liquid. A liquid discharging pipe is also arranged on the chromatographic column assembly; the cleaning mechanism is arranged on the outer wall of the inner centrifugal barrel. By arranging the cleaning mechanism, grease on the inner wall of the inner centrifugal cylinder can fall into a baffle along the cylinder wall after being stripped through ultrasonic waves; by arranging the circulating mechanism, a mixed solution obtained by remixing lipids at the bottom of the cylinder and a supernatant can be introduced into the temporary storage cylinder, so that reprocessing can be performed, a large amount of mixed solution at the bottom is prevented from entering the chromatographic column assembly along with the supernatant to cause influence, and the protein purity is further improved.

The invention discloses a biomass essence and a preparation method thereof.The preparation method comprises the steps that fatty acid and lipoid substances are provided and mixed, a mixed solution is obtained, a dissolving agent is added into the mixed solution, the mixed solution is extracted through an ultrasonic-assisted extraction method, extract liquor is subjected to concentration treatment, and the biomass essence is obtained. Carrying out drying treatment on the concentrated extraction liquid by using nitrogen gas flow to obtain raw material powder; mixing the raw material powder with a surfactant, adding the mixture into deionized water, and carrying out homogenization treatment to obtain an emulsion; mixing the emulsion with a trace metal ion source to obtain a lipid-metal compound, removing insoluble impurities and large-particle substances through centrifugal separation to obtain supernate, and purifying the supernate through an ultrafiltration membrane to obtain primary essence; and adjusting the primary essence to obtain the biomass essence. The purity and the stability of the biomass essence are remarkably improved while the content of the high-activity components is kept.