407 results about "Esterase" patented technology

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

The invention relates to a washing and / or cleaning agent, comprising at least one peptide and at least one enzyme which has hydrolytic activity on an ester bond, preferably selected from lipase and esterase, in particular esterase with lipolytic activity, particularly preferably lipase, wherein the peptide is suitable for adhesion to a fat- and / or oil-containing surface or to a surface coated with fat and / or oil or to a surface provided with at least one fat- and / or oil-containing soil, wherein the surface consists of hard surfaces, more preferably surfaces containing or consisting of ceramic (e.g. porcelain, earthenware), metal, steel, stainless steel, plastic, glass, natural and artificial stone, painted and enamelled surfaces, wood, laminate, linoleum and mixtures thereof, particularly preferably tableware (preferably made of ceramic such as porcelain or earthenware and plastic), metal (e.g.cutlery or pots) or glass and mixtures thereof, particularly preferably tableware (preferably made of ceramic such as porcelain or earthenware and plastic), metal (e.g. cutlery or pots) or glass, and / or textiles, more preferably textiles containing or consisting of cotton, polyester, polyamide, polypropylene and mixtures thereof, particularly preferably cotton, polyester and mixtures thereof. Furthermore, the invention relates to methods for cleaning hard surfaces and / or textiles, and to the use of such peptides and agents for improving the cleaning performance on at least one greasy and / or oily soil.

The invention discloses a p-nitrophenyl ethyl esterase mutant with high enantioselectivity as well as a construction method and application of the p-nitrophenyl ethyl esterase mutant. The p-nitrophenyl ethyl esterase mutant is obtained by mutating an amino acid sequence of wild p-nitrophenyl ethyl esterase pnbA as shown in SEQ ID NO.1 according to one or a combination of more of the following modes: leucine at the 273rd site is mutated into aspartic acid, phenylalanine at the 314th site is mutated into histidine, and leucine at the 362nd site is mutated into arginine. The mutant pnbA-L273D / F314H / L362R provided by the invention shows optimal catalytic performance (E = 47.16) for racemization-acetic acid pinyl hydrate, the enantiomeric excess value of the mutant is 95.13%, the conversion rate of 1S, 5R-acetic acid pinyl hydrate is 90.42%, the enantioselectivity of the mutant is obviously higher than that of a wild type, and the mutant has great application potential in biological catalytic synthesis of monocyclic monoterpenoid chiral alcohols.

The present disclosure concerns polypeptide having fumonisin esterase activity exhibiting increased fumonisin esterase activity, when measured at a temperature of at least 37° C., when compared to the control polypeptide comprising the amino acid of SEQ ID NO: 3. The polypeptides of the present disclosure include one or more amino acid variations which contribute to the increase fumonisin esterase activity. The present disclosure also includes compositions comprising the polypeptide having fumonisin esterase activity, methods for detoxifying a fumonisin mycotoxin as well as processes for making the compositions comprising the polypeptide having fumonisin esterase activity.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a microbacterium strain and preparation and application of a microbial agent of the microbacterium strain. The invention discloses a strain of Microbacterium zhangzhouensis GSS18, which is preserved in Guangdong Microbial Culture Collection Center on November 14, 2022, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.62978. The strain of Microbacterium zhangzhouensis GSS18 disclosed by the invention has the advantages that the preservation number is CGMCC No.62978; compared with the prior art, the Microbacterium zhangzhouensis GSS18 disclosed by the invention can secrete esterase and lipoid esterase, and can be used for accelerating the rapid conversion of lipid substances of the compost and promoting the compost humus process.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The present disclosure provides polypeptides having a cholesterol-lowering function in a soybean protein hydrolysate, a preparation method thereof, and uses thereof. The polypeptides are derived from peptide fragments having a molecular docking “binding energy” value of less than −1.2 kcal with cholesterol esterase. The peptide fragments of the polypeptides that are subjected to molecular docking with cholesterol esterase are derived from 23 polypeptides having a Peptide Ranker bioactivity score of greater than 0.9. The polypeptides possess a significant blood-lipid-lowering function.

The present application belongs to the technical field of enzyme engineering and plastic degradation, and particularly relates to an esterase, a preparation method thereof and application thereof in plastic degradation. Genome sequencing analysis is performed on a screened polyester plastic degrading strain, and an esterase 8GL004290 is successfully screened, which belongs to the alpha / beta hydrolase family. It is verified through experiments that the polyester degrading enzyme provided by the present application can not only degrade polyester model small molecule substrates, but also exhibits good degradation capacity for different polyester plastics. The esterase 8GL004290 has the highest degradation activity on a polyurethane substrate, and the substrate types are widely covered. Therefore, the present application has extremely important application value for the biodegradation treatment of mixed plastic waste.

The invention discloses a derivative of a rice bran extract as well as a preparation method and application of the derivative. A C-3 benzoate prodrug system is constructed, the transmembrane absorption efficiency is remarkably improved by optimizing the oil-water partition coefficient, fixed-point slow release of active ingredients is achieved through inflammatory tissue esterase, and high bioavailability and low irritation are both considered. Meanwhile, C-25 fluorine atoms are introduced to serve as biological electron isosteres, and target affinity is enhanced by means of the unique electronic effect and polar hydrophobicity of the C-25 fluorine atoms; spatial conformation is locked through side chain saturation, so that the compound shows anti-inflammatory and tissue repair activity which is obviously superior to that of natural cycloartanol under the same dosage.

The invention relates to the technical field of polyester yarns, in particular to a high-strength polyester yarn and a preparation method thereof.The method comprises the steps that PET slices with the intrinsic viscosity of 0.85-1.00 dL / g are subjected to melt spinning and cooled through circular blowing to obtain nascent fibers, the nascent fibers are subjected to surface activating treatment through low-temperature plasma or esterase bio-enzyme, and the high-strength polyester yarn is obtained; the preparation method comprises the following steps: firstly, activating fibers, then carrying out graft copolymerization reaction on the activated fibers and graft monomer solutions such as fluorinated acrylate and acrylic acid, and finally, cleaning, drying, carrying out thermal network and winding on the composite fibers, so that the prepared polyester yarn has the breaking strength of more than or equal to 7.7 g / D, the dry heat shrinkage rate of less than or equal to 3.5% and the shrinkage tension of less than or equal to 0.03 g / D, has the high functional characteristics of lasting super-hydrophobicity, antibacterial property and the like, is excellent in washing resistance, and is suitable for industrial production. The problem that functionalization is difficult due to surface inertia of the polyester fiber is solved, and the mechanical property and the surface characteristic of the polyester fiber are comprehensively improved.

The invention discloses a rapid screening and detecting method for vegetable pesticide residues, a multi-dimensional judgment basis is provided for pesticide residue detection by combining a PCR amplification result with the inhibition rate of acetylcholin esterase and butyrylcholin esterase, different types of pesticides may induce different gene expression changes, and the detection result is accurate. According to the rapid screening and detecting method for the pesticide residues in the vegetables, the accuracy of a detection result is improved, meanwhile, the variety of the pesticide residues in the vegetables can be accurately determined, the defect that specific pesticide varieties are difficult to distinguish by purely relying on an enzyme inhibition method is overcome, and the detection result is more targeted and accurate.

The application relates to a preparation method of a biosensor. The application relates to a preparation method of a portable pesticide detection biosensor based on acetylcholinesterase (AChE)@poly(allylamine hydrochloride)-hydrogen bond organic framework (PAH-HOF) and application of the biosensor in on-site rapid chlorpyrifos detection, and belongs to the technical field of biosensors. According to the method, AChE is combined with poly(allylamine hydrochloride) (PAH) to change the surface charge and promote self-assembly of the AChE and the hydrogen bond organic framework (HOF), so that an AChE@PAH-HOF composite material with high catalytic activity, high embedding rate and high stability is formed; then the material is integrated into a water-retaining glycerol-sodium alginate hydrogel (G-SA) matrix to construct a disc-shaped sensor. The sensor can be attached to the surface of plants and used for long-term in-situ monitoring of organophosphorus pesticides. In combination with an image processing algorithm, the system realizes accurate tracking of the chlorpyrifos degradation process on the surface of tomato plants, and the detection limit reaches 1 ng / mL. The application provides non-invasive and low-cost technical support for crop health management and sustainable precision agriculture.

The antioxidant activity of alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) and of alpha-tocopherol succinate (TS) has been examined in isolated hepatocytes and microsomal fractions from rat liver. Both TPGS and TS require esterase activity to yield free alpha-tocopherol and, hence, antioxidant activity. TPGS and TS consistently exerted a more effective antioxidant protection than an equivalent amount of directly-added free alpha-tocopherol. The low antioxidant efficiency of directly added free alpha-tocopherol in such water-based experimental systems as used here seems to be due to its extreme hydrophobicity. TPGS, on the other hand, is an extremely hydrophilic compound which is being examined as a useful source of alpha-tocopherol in certain clinical situations and is here shown to be a convenient and effective source for experimental studies into lipid peroxidation and antioxidant mechanisms.

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

The application discloses a thermophilic bacteria-derived esterase mutant and application thereof. The application provides a protein, wherein the amino acid residue at the 211th position in the amino acid sequence shown in SEQ ID NO: 4 is mutated, and the amino acid residues at other positions are not changed, so that a protein with PBAT hydrolytic enzyme activity is obtained; or the amino acid residues at the 211th and 256th positions in the amino acid sequence shown in SEQ ID NO: 4 are mutated, and the amino acid residues at other positions are not changed, so that a protein with PBAT hydrolytic enzyme activity is obtained; the wild type Tcur-lipase is mutated by using structure analysis and site-directed mutagenesis technology, and the Tcur-lipase mutants N211A and N211A / D256A are obtained; the Tcur-lipase mutant improves the degradation efficiency of PBAT, and has a good industrial application prospect.

The invention relates to the field of blood products, in particular to a preparation method of human activated C1 esterase. The preparation method comprises the following steps: carrying out contact adsorption on cryoprecipitate-removed human plasma and anion exchange resin, washing and eluting to obtain an eluent containing C1 proesterase, carrying out membrane filtration, and carrying out ultrafiltration desalination treatment; adding an anionic substance into the eluent, incubating in vitro to effectively activate the C1 esterase, and filtering by a membrane to obtain a feed liquid containing the activated C1 esterase; and carrying out purification treatment on the feed liquid to obtain the human activated C1 esterase, and carrying out cryopreservation. According to the method, an anion substance is introduced into an eluent system obtained through anion exchange, mild and effective activation of the C1 proesterase is achieved under the condition of not depending on foreign proteolytic enzyme, the risk of non-specific hydrolysis and self-cutting inactivation is remarkably reduced, good synergy is formed with a follow-up purification process, and the method is suitable for industrial production. The human activated C1 esterase product with relatively high activity and good stability is obtained.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

The present invention relates to novel esterases, and more particularly to esterase variants having improved activity and / or improved thermal stability compared to the parent esterase of SEQ ID NO: 1 or SEQ ID NO: 2 under acidic conditions and their use for degrading polyester-containing materials, such as plastic articles. The esterase of the present invention is particularly suitable for degrading polyethylene terephthalate and materials containing polyethylene terephthalate under acidic conditions.

The present application belongs to the technical field of pharmaceutical therapy, and particularly relates to an amide-substituted tryptophan ketone derivative, a preparation method and application thereof. The structural formula of the tryptophan ketone derivative is shown in formula (A) or formula (B). In the formula, n=1 or 2, and R is any one of dimethylamine group, diethylamine group, morpholine group, 1-methylpiperazine group, cyclopropylamine group and 4-hydroxypiperidyl group. The compound can selectively inhibit the activity of acetylcholinesterase, and has the potential to develop into a drug for treating Alzheimer's disease due to the abilities of self-aggregation and the like. 1‑42 ​

The application provides a high-activity pectin methylesterase mutant designed based on a diffusion model, and preparation and application of the pectin methylesterase mutant, and the amino acid sequence of the pectin methylesterase mutant PME-DS12 is shown as SEQ ID NO:2. The application re-designs the structure of the pectin methylesterase by using RFdiffusion generated diffusion model, and a mutant PME-DS12 with significantly improved enzyme activity is screened. Compared with the wild-type pectin methylesterase, the enzyme activity of PME-DS12 is improved by about 6.3 times, and PME-DS12 has higher industrial application value. The mutant has wide application prospects in the fields of food processing, textile and the like. Especially in the aspect of flavor substance generation, the enzyme can provide the generation of aroma lipid substances by degrading macromolecular substances in food, so as to improve the aroma quality of products; and the enzyme can also be used in the papermaking industry, including biological bleaching of paper pulp, reduction of use of chemical reagents and reduction of environmental pollution.

The invention discloses a plurality of mutants of esterase E7, comprising: esterase E7-F145L, the amino acid sequence of which is as shown in SEQ ID NO.5; the amino acid sequence of the esterase E7-F145A is as shown in SEQ ID NO. 4; the amino acid sequence of the esterase E7-Q199F is as shown in SEQ ID NO. 8. The mutant of the esterase E7 is applied to preparation of glyceride, preferably EPAMG. Through semi-rational design, site-directed mutagenesis is carried out on a bridge-shaped structure of esterase E7 from stenotrophomonas maltophilia, a plurality of mutants with remarkably improved catalytic activity are obtained, the effect of the mutant E7-F145L is optimal, the reaction efficiency is greatly improved, the high specificity of a catalytic product is reserved, and the application potential is huge.

本发明公开了一种基于静电驱动的双酶级联放大荧光传感器及其制备方法和应用，所述纳米酶荧光传感器由探针Azo‑Bodipy 685、胆碱氧化酶（CHO）和氯化乙酰胆碱（ACh）组成。本发明基于静电吸附原理，提出静电络合共固定化‑酶级联反应协同策略，构建一种新的静电驱动荧光传感器，利用ACh的季铵盐正电荷与CHO表面负电荷的静电作用，在特异性荧光探针Azo‑Bodipy 685表面构建纳米尺度空间限域的酶反应微环境。当有机磷农药抑制乙酰胆碱酯酶（AChE）时，氯化胆碱生成减少导致CHO催化产生的过氧化氢量下降，荧光信号强度减弱，一系列荧光强度的变化可以实现检测AChE活性和测定有机磷农药残留含量。

The application provides a recombinant cholesteryl esterase, a polynucleotide thereof, a recombinant plasmid, an expression system and application. The application belongs to the technical field of protein engineering, and specifically, the amino acid sequence of the recombinant cholesteryl esterase provided by the application is shown as SEQ ID No. 1, and the nucleotide sequence for encoding the recombinant cholesteryl esterase is shown as SEQ ID No. 2. The sequence of the recombinant cholesteryl esterase provided by the application has a full length of 482 amino acids, is derived from Aspergillus ruber, has the advantage of high thermal stability, and has a thermal stability deviation of less than 10% after thermal storage at 37 DEG C for 11 days and after 30 days of on-board opening, and has a wide application prospect in the field of in-vitro diagnostic reagents.

This invention discloses an enzyme-responsive silk fibroin nanovesicle, its preparation method, and its application, comprising the following steps: silk fibroin is mixed with a hydrophobic acylation reagent containing enzymatically cleavable chemical bonds and a catalyst for a grafting reaction to obtain amphiphilic modified silk fibroin; the amphiphilic modified silk fibroin is mixed with hair care active ingredients in an aqueous phase, and self-assembly is induced to form nanovesicles encapsulating the active ingredients through ultrasonic treatment or high-pressure homogenization. This invention uses silk fibroin as the main body to provide a biocompatible framework and self-assembly basis. Hydrophobic groups are introduced through chemical modification, and these hydrophobic groups are connected to the silk fibroin backbone through enzymatically cleavable chemical bonds. These chemical sites can be recognized and cleaved by sebaceous lipase secreted by the scalp sebaceous glands or other specific lipases highly expressed in the hair follicle microenvironment, achieving specific enzymatic release of the nanovesicles in the hair follicle microenvironment.

The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and / or hydrolases.

The invention belongs to the technical field of antibacterial products, and particularly relates to a female private part antibacterial composition. Through the synergistic effect of the white willow bark extract, the melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedar wood oil, the prepared antibacterial composition has an excellent antibacterial effect, is mild and non-irritant, and can solve the problems that an existing female private part antibacterial agent is limited in antibacterial effect, and the antibacterial effect is poor; and gynecological diseases can be aggravated after long-term use. Wherein the white willow bark extract is prepared through enzymolysis of a compound enzyme composed of cellulase, feruloyl esterase and alkaline protease, the enzymolysis efficiency can be improved, and then the release rate of active ingredients in white willow bark is increased. The preparation method of the antibacterial composition is simple and convenient in operation process and suitable for industrial production. The antibacterial composition is applied to preparation of female private part care products and has good application value.