683 results about "Esterase" patented technology

The invention discloses a fusion enzyme capable of efficiently degrading PET (polyethylene terephthalate) plastic and application of the fusion enzyme. The invention provides a recombinant fusion enzyme. The recombinant fusion enzyme comprises a mutant KL-MHETase of thermophilic carboxylesterase Est30 and a PET (Polyethylene Terephthalate) degrading enzyme FAST-PETase, the amino acid sequence of the mutant KL-MHETase of the thermophilic carboxylesterase Est30 is as shown in SEQ ID NO. 3; the amino acid sequence of the FAST-PETase is as shown in the 284th to 546th amino acids of SEQ ID NO.1 (Sequence Identifier Number 1). The fusion enzyme is used as a biocatalyst, the activity of catalyzing hydrolysis of MHET at 50 DEG C is 52.6 times that of FAST-PETase, and the fusion enzyme has high enzyme activity and thermal stability. PET degradation is catalyzed at the temperature of 50 DEG C, the conversion rate of PET is 90% after 48 hours of reaction, and meanwhile, the TPA content in the product is close to 100%.

The invention discloses a high-activity and high-thermal-stability MHET hydrolase mutant and application thereof. The invention provides a mutant protein of thermophilic carboxylesterase, which is shown as 1) or 2): 1) the protein shown as 1) is a protein obtained by mutating amino acid residues at the 130th site and the 171th site of wild thermophilic carboxylesterase shown as SEQ ID NO.2 or a sequence corresponding to the SEQ ID NO.2 and keeping other amino acid residues unchanged; (2) the protein is obtained by mutating amino acid residues at the 127th site, the 130th site and the 171th site of wild thermophilic carboxylesterase and keeping other amino acid residues unchanged. Experiments prove that the activity of the modified mutant for catalyzing MHET hydrolysis at the temperature of 50 DEG C is improved by 95 times compared with that of a wild type, and the modified mutant has higher enzyme activity and thermal stability and is the first enzyme capable of efficiently catalyzing MHET hydrolysis at the temperature of 50 DEG C at present.

The invention discloses feruloyl esterase fermentation preparation and application of feruloyl esterase in polyester plastic degradation, and belongs to the field of environmental science. According to the method, feruloyl esterase FAE4 derived from Anerocephalum thermophilum is prepared through fermentation, and is compounded with polyester degrading enzymes which are different in source and are used for degrading polyester plastic oligomers, so that a compound reaction system which remarkably improves the degradation efficiency of poly (butylene adipate-co-terephthalate) or poly (butylene adipate-co-terephthalate) (PBAT) and the content proportion of a final product is obtained; good industrial prospects are realized.

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to an esterase mutant for degrading ethyl carbamate and application of the esterase mutant, the esterase mutant has an amino acid sequence as shown in SEQ ID NO.1 which is subjected to site mutation, the site mutation is any one or more site combined mutation of V129S, V161L or I229M, and the amino acid sequence of the esterase mutant is shown in SEQ ID NO.1. The V129S site mutation is that valine at the 129 site is mutated into serine, the V161L site mutation is that valine at the 161 site is mutated into leucine, and the I229M site mutation is that isoleucine at the 229 site is mutated into methionine. Mutation is carried out through a site-directed mutagenesis method, so that the amino acid sequence of the esterase mutant V129S / I229M is changed, the autoplasmic structure and function of eggs are changed, the esterase mutant V129S / I229M with the ethyl carbamate hydrolysis rate increased to 6.55 times in an acid environment is finally obtained, and the esterase mutant V129S / I229M has high industrial application value.

The invention relates to the field of microorganisms, and discloses an Aspergillus tubingensis strain and application thereof in preparation of meal seasonings, the Aspergillus tubingensis strain is not subjected to mutagenesis and acclimatization and has the capacity of high yield of glutaminase, feruloyl esterase, glucosidase, pectinase and protease, and when the Aspergillus tubingensis strain is applied to soy sauce brewing, the yield of soy sauce is increased. The high-value utilization of the meal raw materials can be realized, the amino acid content in the soy sauce is increased, and the flavor and nutritional value of the soy sauce are enhanced.

The invention relates to a STING agonist prodrug compound, a liposome preparation and a preparation method thereof. Specifically, the invention relates to an STING agonist prodrug compound shown in a general formula (I) or a pharmaceutically acceptable salt thereof, a liposome preparation thereof and application of the STING agonist prodrug compound or the pharmaceutically acceptable salt and the liposome preparation in preparation of drugs for treating STING-related diseases. The prodrug compound disclosed by the invention can be rapidly hydrolyzed under the induction of esterase to release an active ingredient MSA-2, and has a good STING protein excitation function. # imgabs0 #

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention discloses a biological catalytic synthesis method of norambrox ether, which comprises the following steps: step 1, starting from trans-nerolidol, carrying out carroll rearrangement reaction to synthesize farnesyl acetone; 2, in the presence of a cofactor, converting farnesyl acetone into high farnesol acetate under catalysis of Bayer-Villiger monooxygenase; step 3, catalyzing the deacetylation of the high-farnesol acetate by using esterase to obtain EE-high-farnesol; and step 4, carrying out enzymatic conversion on farnesol by using the squalene hopaene cyclase mutant to obtain norambrox ether. The transformation process is safe and non-toxic, Bayer-Villiger monooxygenase which is new with a key enzyme, natural co-expressed esterase, alcohol dehydrogenase and squalene and hopaene cyclase mutants matched with the system are used for synergistic transformation, so that not only is the coenzyme for oxidation reaction promoted to be recycled, but also the conversion efficiency is improved; conversion from farnesyl acetone to norambrox ether can be efficiently and specifically catalyzed, and a final product can be directly obtained from a reaction system without separation and purification of an intermediate.

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

The invention relates to a washing and / or cleaning agent, comprising at least one peptide and at least one enzyme which has hydrolytic activity on an ester bond, preferably selected from lipase and esterase, in particular esterase with lipolytic activity, particularly preferably lipase, wherein the peptide is suitable for adhesion to a fat- and / or oil-containing surface or to a surface coated with fat and / or oil or to a surface provided with at least one fat- and / or oil-containing soil, wherein the surface consists of hard surfaces, more preferably surfaces containing or consisting of ceramic (e.g. porcelain, earthenware), metal, steel, stainless steel, plastic, glass, natural and artificial stone, painted and enamelled surfaces, wood, laminate, linoleum and mixtures thereof, particularly preferably tableware (preferably made of ceramic such as porcelain or earthenware and plastic), metal (e.g.cutlery or pots) or glass and mixtures thereof, particularly preferably tableware (preferably made of ceramic such as porcelain or earthenware and plastic), metal (e.g. cutlery or pots) or glass, and / or textiles, more preferably textiles containing or consisting of cotton, polyester, polyamide, polypropylene and mixtures thereof, particularly preferably cotton, polyester and mixtures thereof. Furthermore, the invention relates to methods for cleaning hard surfaces and / or textiles, and to the use of such peptides and agents for improving the cleaning performance on at least one greasy and / or oily soil.

The invention discloses a p-nitrophenyl ethyl esterase mutant with high enantioselectivity as well as a construction method and application of the p-nitrophenyl ethyl esterase mutant. The p-nitrophenyl ethyl esterase mutant is obtained by mutating an amino acid sequence of wild p-nitrophenyl ethyl esterase pnbA as shown in SEQ ID NO.1 according to one or a combination of more of the following modes: leucine at the 273rd site is mutated into aspartic acid, phenylalanine at the 314th site is mutated into histidine, and leucine at the 362nd site is mutated into arginine. The mutant pnbA-L273D / F314H / L362R provided by the invention shows optimal catalytic performance (E = 47.16) for racemization-acetic acid pinyl hydrate, the enantiomeric excess value of the mutant is 95.13%, the conversion rate of 1S, 5R-acetic acid pinyl hydrate is 90.42%, the enantioselectivity of the mutant is obviously higher than that of a wild type, and the mutant has great application potential in biological catalytic synthesis of monocyclic monoterpenoid chiral alcohols.

The invention discloses a method for preparing uric acid-reducing diglyceride through enzymolysis and a product and application thereof, and belongs to the technical field of diglyceride production, the method comprises the following steps: (1) mixing vegetable oil, glycerol, water and an enzyme preparation for reaction to obtain a reaction solution; (2) standing and layering the reaction liquid, and collecting supernatant; (3) performing distillation separation on the supernatant, and performing decoloration and deodorization to obtain diglyceride; the enzyme preparation in the step (1) is prepared from lipase Lipase, esterase Esterase and phospholipase C in a weight ratio of (5-10): (0.1-0.3): 1. Compared with the prior art, the method disclosed by the invention is simple and low in cost, and the prepared diglyceride has more advantages in the aspect of reducing uric acid.

The invention provides a blood fat reducing polypeptide as well as a screening method and application thereof, and belongs to the field of polypeptides. The blood fat reducing polypeptide provided by the invention comprises at least one of 116 polypeptides with amino acid sequences of FFPFF, VFFFPF, PFFFF, AFFFPFF, LFFFPFY and the like. The blood fat reducing polypeptide provided by the invention can effectively reduce blood fat, has no obvious toxic or side effect, shows good inhibitory activity on steroid esterase and lipase in an in-vitro enzyme activity test, and also can effectively reduce blood fat in an animal in-vivo experiment; and no obvious toxic or side effect is shown in toxicity prediction and animal in-vivo models.

The present invention discloses a pectin methylesterase mutant ΔDdPemA, its encoding gene, preparation method, and application. The pectin methylesterase mutant ΔDdPemA is obtained by mutating the amino acid at position 200 in the center of the substrate binding pocket of the wild-type pectin methylesterase DdPemA from a high-molecular-weight, branched valine to a low-molecular-weight alanine, and by mutating the amino acid at position 339 at the substrate inlet and outlet of the substrate binding pocket from a high-molecular-weight, branched valine to a basic lysine. The amino acid sequence and nucleotide sequence of the wild-type pectin methylesterase DdPemA are SEQ ID NO. 1 and SEQ ID NO. 2, respectively. The mutant enzyme provided by the present invention has significantly improved enzyme activity under weakly acidic conditions, solving the problem of insufficient catalytic activity of the wild-type pectin methylesterase under weakly acidic conditions and creating favorable conditions for the application of the enzyme in the fields of food, textiles, and feed processing.

The invention discloses a preparation method of a carbon dot nano-enzyme, which comprises the following steps: dissolving oxalic acid, polyethyleneimine and ferric nitrate in ultrapure water, heating, mixing and reacting, cooling to room temperature, centrifuging, filtering, dialyzing, and taking supernate to obtain the carbon dot nano-enzyme. The prepared oxalic acid carbon dot nano-enzyme is matched with acetylcholin esterase, acetylcholine, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2 to construct a colorimetric and fluorescent dual-mode detection method for glyphosate in fruits and vegetables. The oxalic acid carbon dot nano-enzyme is prepared by doping ferric nitrate and tetraethylenepentamine into oxalic acid serving as a precursor, the oxalic acid carbon dot nano-enzyme has excellent peroxidase-like activity, a colorimetric and fluorescent dual-mode detection method of glyphosate is constructed through the oxalic acid carbon dot nano-enzyme, dual-mode detection results can be mutually verified, and the detection result is accurate. And the accuracy and the reliability of a detection result are remarkably improved.

The present disclosure concerns polypeptide having fumonisin esterase activity exhibiting increased fumonisin esterase activity, when measured at a temperature of at least 37° C., when compared to the control polypeptide comprising the amino acid of SEQ ID NO: 3. The polypeptides of the present disclosure include one or more amino acid variations which contribute to the increase fumonisin esterase activity. The present disclosure also includes compositions comprising the polypeptide having fumonisin esterase activity, methods for detoxifying a fumonisin mycotoxin as well as processes for making the compositions comprising the polypeptide having fumonisin esterase activity.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a microbacterium strain and preparation and application of a microbial agent of the microbacterium strain. The invention discloses a strain of Microbacterium zhangzhouensis GSS18, which is preserved in Guangdong Microbial Culture Collection Center on November 14, 2022, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.62978. The strain of Microbacterium zhangzhouensis GSS18 disclosed by the invention has the advantages that the preservation number is CGMCC No.62978; compared with the prior art, the Microbacterium zhangzhouensis GSS18 disclosed by the invention can secrete esterase and lipoid esterase, and can be used for accelerating the rapid conversion of lipid substances of the compost and promoting the compost humus process.

The invention provides a biological enzyme preparation for rapidly reducing pesticide residues and a preparation method thereof, and belongs to the technical field of pesticide residue degradation. The invention provides a biological enzyme preparation capable of rapidly reducing pesticide residues. The biological enzyme preparation is prepared from the following components in parts by weight: 10 to 20 parts of phytase, 10 to 50 parts of acetylcholin esterase, 5 to 10 parts of protease, 10 to 20 parts of tyrosinase, 1 to 3 parts of radix platycodonis extract and 1 to 3 parts of aloe extract. According to the biological enzyme preparation capable of rapidly and efficiently reducing the pesticide residues, the removal rate of organophosphorus pesticides on fruits and vegetables is 95% or above, the removal rate of pyrethroid pesticides on the fruits and vegetables is 70% or above, and the biological enzyme preparation is simple in formula, easy to clean and free of secondary residues.

The invention discloses a lactobacillus mucus fermentation strain and application thereof, the lactobacillus mucus fermentation strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on February 21, 2025, and the preservation number is GDMCCNO: 65936. The feruloyl esterase is separated and screened from white sour soup in Kailing in southeast of Guizhou province, has feruloyl esterase activity, and shows good blood fat reducing and fatty liver treating capabilities in a HepG2 high-fat cell model and a high-fat zebra fish model. The compound has a good prospect in the development of products for preventing or treating hyperlipidemia and fatty liver and relieving obesity.

The invention relates to the technical field of biological materials, in particular to a cyclic peptide derivative and a preparation method and application thereof. The cyclopeptide derivative provided by the invention simultaneously comprises a hydrophobic cyclopeptide structure and a hydrophilic pantothenic acid structure which are combined through a covalent ester bond; due to the structure, excellent stability is achieved, and the problems that pantothenic acid is unstable and irritant are effectively solved; after entering the skin, the derivative can be hydrolyzed into pantothenic acid under the action of esterase, and can play a role without oxidative conversion of panthenol. Secondly, the cyclic peptide derivative disclosed by the invention has good biological safety; in an inflammatory cell model, the derivative can more effectively reduce expression of inflammatory factors, and antioxidant activity research shows that the derivative has a strong free radical inhibition effect and can exert excellent anti-inflammatory and antioxidant effects.

Provided are a polypeptide having the function of lowering cholesterol in a soybean protein hydrolysate, and a preparation method therefor and the use thereof. The polypeptide is derived from a peptide fragment having a binding_energy value of < -1.2 Kcal docked to a cholesterol esterase molecule, and the peptide fragment of the polypeptide subjected to molecular docking to cholesterol esterase is derived from 23 polypeptides with the biological activity score of Peptide Ranker > 0.9. Therefore, the polypeptide has a significant blood-lipid-lowering function.

The invention provides a triglyceride determination kit, the kit comprises a reagent 1 and a reagent 2, the reagent 1 comprises the following main components: piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH 6.8), glycerophosphate oxidase (GPO), peroxidase (POD), triadenosine phosphate (ATP), chromogen, a surfactant, a stabilizer and a preservative; and the reagent 2 comprises the following main components: a piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH is 6.5), lipoprotein esterase (LPL), glycerol kinase (GK), 4-aminoantipyrine (4-AA), a surfactant, a stabilizer and a preservative. By optimizing a chromogen substrate in the reagent and adding potassium ferrocyanide, the linear range of the prepared kit is wider, and the bottle opening test is more stable.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention discloses cyclohexene formate esterase, a mutant of cyclohexene formate esterase and application of cyclohexene formate esterase in production of (S)-3-cyclohexene-1-formic acid. According to the cyclohexene formate esterase and the mutant disclosed by the invention, 100-400g / L of racemic 3-cyclohexene-1-methyl formate can be split by taking wet thalli subjected to induced expression or crude enzyme liquid obtained by ultrasonically crushing the wet thalli as a catalyst, so that (S)-3-cyclohexene-1-formic acid with chemical purity and optical purity reaching 99% or above is synthesized. The enzyme activity of the cyclohexene formate esterase mutant provided by the invention is further improved, the reaction time and the catalyst dosage are obviously reduced compared with those of a substrate with the same catalysis concentration before mutation, the reaction condition is mild, the operation is relatively simple and convenient, and the cyclohexene formate esterase mutant has a very good industrial application prospect.

The invention discloses a preparation method of a pilose antler mushroom extract and application of the pilose antler mushroom extract in products with nitrite adsorption and / or lipid lowering efficacy, SDF in pilose antler mushroom is extracted, it is found that the content of SDF in pilose antler mushroom is high, the pilose antler mushroom has good in-vitro lipid lowering activity, and the pilose antler mushroom is a high-quality edible mushroom raw material capable of being used for lipid lowering functional food development. A biological activity experiment shows that the pilose antler mushroom SDF extract can well remove DPPH and ABTS free radicals, adsorb nitrite and inhibit the activity of cholesterol esterase and pancreatic lipase, and shows good oxidation resistance, nitrite adsorption and lipid-lowering activity.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

The invention discloses a galactose-masked aminoquinoline compound and application thereof, the structural formula of the galactose-masked aminoquinoline compound is # imgabs0, and R is acetyl, propionyl or butyryl. After being subjected to hydrolysis deacetylation, propionylation or butyrylation by esterase in cells, the compound can be activated by senescence-related beta galactosidase in senescence cell lysosome to release aminoquinoline (NCQ) so as to inhibit the survival of senescence cells in vivo or in vitro.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present invention relates to novel esterases, more particularly to esterase variants having improved activity and / or improved thermostability compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.