421 results about "Lipidome" patented technology

The invention relates to a dialkyl imidazole bionic lipid compound and a preparation method and application thereof.The structure of the dialkyl imidazole bionic lipid compound imitates natural phospholipid design, alkyl with no less than 10 carbon atoms is modified on the fourth site and the fifth site of imidazole, and primary amines with different alkyl chain lengths are modified on the second site; the dialkyl imidazole bionic lipid compound is mixed with a therapeutic drug and other auxiliary materials to prepare lipid nanoparticles loaded with the therapeutic drug, and the lipid nanoparticles can be used as a drug delivery system for preparation of brain-targeted drugs. The dialkyl imidazole bionic lipid has the function of dynamically regulating and controlling the blood-brain barrier, and can realize reversible opening of the blood-brain barrier; the formed drug-loaded lipid composition can pass through a blood brain barrier and well realize brain-targeted delivery of loaded therapeutic drugs; the drug-loaded lipid composition composed of the dialkyl imidazole bionic lipid belongs to a new generation of bionic nano-drug carriers, and provides a new strategy for realizing brain-targeted delivery of different types of drugs.

The present application relates to a kind of dihydrocarbylimidazole biomimetic lipid compound and its preparation method and application, dihydrocarbylimidazole biomimetic lipid compound structure imitates natural phospholipid design, modification is not less than 10 carbon atoms alkyl on the 4th and 5th of imidazole, and modification is different alkyl chain length primary amine on 2nd position;Dihydrocarbylimidazole biomimetic lipid compound is mixed with therapeutic drug and other adjuvant, can be prepared to be loaded in the lipid nanoparticle of therapeutic drug, can be used as drug delivery system for the preparation of brain-targeted drug.Dihydrocarbylimidazole biomimetic lipid has the function of dynamic control blood-brain barrier, can realize the reversible opening of blood-brain barrier;Formed drug-loaded lipid composition can cross blood-brain barrier and better realize the brain-targeted delivery of loaded therapeutic drug;Drug-loaded lipid composition consisting of dihydrocarbylimidazole biomimetic lipid belongs to a new generation of biomimetic nanomedicine carrier, provides new strategy for realizing the brain-targeted delivery of different kinds of drugs.

The invention relates to a construction method of a metabolic syndrome phlegm syndrome lipid metabolism characteristic diagnosis model. The construction method comprises the following steps: S1, acquiring a serum sample; s2, carrying out lipid metabolite analysis through a full-quantitative lipidomics formula; s3, carrying out primary screening on differential metabolites; s4, optimizing the characteristic indexes by using a random forest algorithm and stepwise regression; and S5, constructing a differential metabolite diagnosis model through Logistic regression analysis. By analyzing differential lipid metabolites of patients with MetS phlegm syndromes and non-phlegm syndromes, the invention reveals that abnormal accumulation of lipid and lipid metabolites may be the core pathological parenchyma of MetS phlegm syndromes. By integrating lipid metabonomics data, using a random forest algorithm, stepwise regression and other methods to screen feature difference lipid metabolites and construct a MetS phlegm syndrome specific diagnosis model, a novel combined biomarker and evidence-based basis are provided for early diagnosis of MetS phlegm syndromes, and a scientific basis can also be provided for objective diagnosis of traditional Chinese medicine phlegm syndromes.

The purpose of the present invention is to provide a lipid composition capable of delivering a nucleic acid such as RNA to a hematopoietic stem / progenitor cell or a mesenchymal stem cell, and a method for delivering a therapeutic agent to a cell using the lipid composition. The present invention provides a lipid composition comprising (A) a therapeutic agent and (B) a lipid nanoparticle conjugated to a targeting molecule wherein the lipid nanoparticle comprises an ionizable lipid and the targeting molecule specifically binds to a marker of hematopoietic stem / progenitor cells or mesenchymal stem cells.

The invention discloses an antibody-modified lipid nanoparticle, a preparation method thereof and application of the antibody-modified lipid nanoparticle as a targeting carrier. The invention provides lipid nanoparticles coupled with an antibody and loaded with nucleic acid. The lipid nanoparticles are characterized in that raw materials of the lipid nanoparticles consist of ionizable lipid, phospholipid, steroidal lipid, PEG lipid and PEG-Mal lipid, the PEG lipid is C16-PEG2k, and the PEG-Mal lipid is C16-PEG2k-Mal, and the PEG-Mal lipid is C16-PEG2k-Mal. The method aims at the key scientific problems of low delivery efficiency, insufficient targeting and the like in the field of in-vivo hematopoietic stem / progenitor cell gene therapy at present. The invention develops a lipid nanoparticle delivery system based on antibody modification, provides a modular antibody targeted delivery platform with high universality, and shows huge clinical transformation potential.

Provided herein are lipid nanoparticles composition comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide. Further provided are lipid nanoparticle compositions comprising a lipid component which comprises: (i) a first ionizable cationic lipid, (ii) a PEG-lipid, wherein the PEG-lipid comprises a PEG-Ceramide selected from: N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C8 PEG5000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]}(C8 PEG2000-Ceramide), N-octanoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C8 PEG750-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)5000]} (C16 PEG5000-Ceramide), N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (C16 PEG200-Ceramide), and N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)750]} (C16 PEG750-Ceramide) (iii) a phospholipid, and (iv) a permanently cationic lipid, an anionic lipid, or a second ionizable cationic lipid separate from the first ionizable cationic lipid.

Provided are a cationic lipid and a preparation method thereof, a lipid composition containing the cationic lipid, and an LNP-drug composition and a preparation thereof containing the lipid composition, especially an LNP-drug composition and a preparation thereof containing the cationic lipid, which have the characteristics of high delivery efficiency and high biocompatibility, thereby improving the therapeutic effect and preventive effect of the drug. The structure of the cationic lipid is shown in general formula (1), and the definitions of the symbols in the formula are as described in the text.

Described herein are compositions, kits, and methods for potent delivery to a cell of a subject. The cell can be of a particular cell type, such as a basal cell, a ciliated cell, or a secretory cell. In some cases, the cell can be a lung cell of a particular cell type. Also described herein are pharmaceutical compositions comprising a therapeutic or prophylactic agent assembled with a lipid composition. The lipid composition can comprise an ionizable cationic lipid, a phospholipid, and a selective organ targeting lipid. Further described herein are high-potency dosage forms of a therapeutic or prophylactic agent formulated with a lipid composition.

Provided herein are lipid nanoparticles composition comprising a lipid component which comprises: (i) a first lipid wherein the first lipid is an ionizable cationic lipid, (ii) a second lipid wherein the second lipid is separate from the first lipid, (iii) a phospholipid, and (iv) a PEG-lipid wherein the second lipid is a lipid having a structural formula S-I'a, S-I'b, or S-I'c. Also, provided herein is a method of treating or preventing a disease or disorder in a subject in need thereof, the method comprising administering an effective amount of the lipid nanoparticle composition disclosed herein.

This disclosure provides a lipid composition and methods of use thereof.

The invention discloses a method for improving the survival rate of probiotics based on cell membrane regulation and control. The method comprises the following steps: adding a lipid component in a probiotic culture process and / or adding the lipid component as a protective agent after probiotic culture, the lipid component added in the probiotic culture process comprises cytidine diphosphate diacylglycerol and / or stearic acid; the lipid component added as the protective agent after culture comprises cardiolipin and / or oleic acid. The method breaks through the research category of a traditional optimized freeze drying process (such as a protective agent and parameters), and key lipid molecules directly related to resistance are screened out through a lipidomics means and establishment of a screening method of stress resistance related lipid molecules; through a dual mode of supplementing key lipid components and precursor substances thereof, the tolerance of the strain is improved from the metabolic level, and the freeze-drying resistance and the survival rate of the strain are remarkably improved.

The invention discloses a double-selenium lipid nanoparticle as well as a preparation method and application thereof. According to the nanoparticle, a skeleton is composed of cationic lipid, neutral phospholipid, cholesterol and functionalized PEG lipid, meanwhile, mRNA and a TLR agonist for coding therapeutic protein are carried, and the nanoparticle is synthesized through an ethanol injection method; the nanoparticle has both active targeting and strong transfection capabilities, the lipid nanoparticle is actively targeted to a tumor microenvironment and is taken by tumor cells by using biotin modified PEG lipid, and rapid'inclusion-lysosome 'escape is realized in the tumor cells by using trifluorogroup modified PEG lipid. Meanwhile, the nanoparticles are degraded in a glutathione / hydrogen peroxide dual-mode, so that the entrapped medicine is quickly and effectively released; the drug delivery system provided by the invention is simple in synthesis method, high in gene transfection efficiency and good in biological safety, and has potential value in anti-tumor research and clinical application.

The invention discloses a nucleic acid medicinal preparation based on dialkyl imidazole cationic lipid as well as a preparation method and application thereof, and belongs to the field of medicinal preparations. The nucleic acid pharmaceutical preparation comprises drug-loaded lipid nanoparticles, and the drug-loaded lipid nanoparticles comprise one or more dihydrocarbyl imidazole cationic lipids. By adjusting lipid composition and proportion in the drug-loaded lipid nanoparticles, efficient loading and delivery of different types of nucleic acid drugs can be realized; the drug-loaded lipid nanoparticles have good biological safety, the preparation process is simple, and large-scale production is easy; the drug-loaded lipid nanoparticle can effectively pass through a blood brain barrier, realizes specific brain-targeted delivery of the loaded nucleic acid drug, and provides a new strategy for realizing brain-targeted delivery of different nucleic acid drugs for treating brain diseases.

The invention provides a method for nondestructive detection of contents of EPA and DHA in adductor muscles of scallops based on Raman spectroscopy, which comprises the following steps: taking a proper amount of gill filament tissues in a survival state of the scallops by using a nondestructive gill filament tissue taking method, preparing into frozen slices, performing Raman laser scanning on the slices to obtain original data, performing pretreatment such as normalization, noise reduction, baseline correction and the like, and determining the content of EPA and DHA in the adductor muscles of the scallops. Selecting a characteristic wavelength, and combining with adductor muscle lipidome data to establish a model for predicting the content of unsaturated fatty acid in adductor muscle in a cross-tissue manner by gill filament Raman data. Compared with an existing method for detecting the content of unsaturated fatty acid in scallops, the method has the advantages that the content of unsaturated fatty acid in rhabdoids of main edible parts of the scallops can be quickly, accurately and simply detected under the condition that the scallops survive, and the detection cost is greatly reduced; an efficient and simple technical means is provided for breeding of shellfish quality characters, and popularization and promotion in comprehensive evaluation and genetic improvement work of aquatic germplasm are facilitated.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

The invention relates to the field of biological medicine, and discloses application of LPC, PC and AA in children sepsis diagnosis and prognosis product development. According to the invention, a high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS) technology is used, plasma samples of a child sepsis patient and a healthy control group are compared, the relationship between lipidome change and pSOFA score is analyzed, and specific lipid molecules closely related to disease severity and prognosis are identified. According to the invention, the following lipid molecules are highlighted as key biomarkers: lysophosphatidylcholine (LPC), phosphatidylcholine (PC) and arachidonic acid (AA). The change of the lipid molecules is closely related to the physiological state of a child sepsis patient, and the lipid molecules can be used for early diagnosis and evaluation of the state of the child sepsis, so that the treatment effect and the accuracy of prognosis evaluation are improved, and a new treatment strategy and a new management method are provided for clinic.

Disclosed herein are modified lipid compositions comprising (a) a structural component comprising one or more lipids selected from the group consisting of soybean-derived lipids, cardiolipin, sphingolipid, ceramide, glucosylceramide, lactosylceramide, galactosylcholesterol, glucosylcholesterol; and modified by (b) ionizable lipids. The present disclosure also includes a method for preparing a modified lipid composition, the method comprises reconstituting (a) a structural component comprising one or more lipids selected from the group consisting of soybean-derived lipids, cardiolipin, sphingolipid, ceramide, glucosylceramide, lactosylceramide, galactosylcholesterol and / or glucosylcholesterol in the presence of (b) ionizable lipids to produce the modified lipid composition, and loading one or more heterologous functional agents into the modified lipid composition.

The invention discloses a lipid composition, lipid nanoparticles, a preparation method of the lipid nanoparticles, a pharmaceutical composition, a pharmaceutical preparation and application. The lipid composition is prepared from a cationic lipid SM-102, a cationic lipid NT-O14B, cholesterol, distearoyl phosphatidylcholine (DSPC) and a phospholipid polyethylene glycol (PEG) derivative. The invention provides the lipid composition which can be applied to preparation of lipid nanoparticles for brain delivery, through optimal combination of specific lipid components, the efficiency of penetrating through the blood brain barrier during LNP drug delivery is improved, reliable technical support is provided for application of siRNA drugs in nervous system diseases, and the lipid composition has the application prospect of promoting treatment of the nervous system diseases.

The invention relates to an mRNA (messenger Ribonucleic Acid) vaccine targeting FomA and application of the mRNA vaccine in immunotherapy of esophageal squamous carcinoma in the technical field of tumor immunotherapy. The problems that in the prior art, a specific targeting vaccine for the fusobacterium nucleatum membrane protein FomA lacks, traditional antibiotics can only kill extracellular bacteria and cannot remove intracellular colonized fusobacterium nucleatum, and cellular immunity cannot be effectively activated to remove fusobacterium nucleatum colonized in tumor tissue; the immunotherapy drug resistance of esophageal squamous carcinoma caused by fusobacterium nucleatum infection is difficult to reverse. According to the technical scheme, an mRNA molecule with a fusobacterium nucleatum FomA extracellular domain tandem repeat sequence and a lipid nanoparticle delivery system are coded, an extramembrane segment tandem repeat immune enhancement technology is adopted for design, two extracellular ring sequences are repeated for 2-5 times and connected through a connector sequence, lipid nanoparticles are composed of optimized quaternary lipids, and the lipid nanoparticles are prepared from the quaternary lipids. And a PD-1 monoclonal antibody is combined to activate FomA specific CD8 + T cell immunoreaction, so that comprehensive removal of intracellular and extracellular bacteria and bacteria targeted immune remodeling are realized.

The invention discloses retinol retinoate-ceramide lipidosome modified by hyaluronic acid and salts thereof and a preparation method of the retinol retinoate-ceramide lipidosome, and belongs to the technical field of cosmetic nano-carriers. The preparation method comprises the steps that retinol retinoate-ceramide lipidosome is prepared, hyaluronic acid is pretreated, and the hyaluronic acid and the salts thereof are combined to prepare the retinol retinoate-ceramide lipidosome. The liposome is modified by hyaluronic acid and salts thereof in a dual-mechanism manner, and purification and stabilization are realized; the preparation method of the retinol retinoate-ceramide liposome comprises the following steps: dissolving phospholipid, a functionalized lipid component with positive ions and amino functional groups, cholesterol, retinol retinoate and ceramide in absolute ethyl alcohol to serve as an organic phase, taking a phosphate buffer solution as a water phase, dropwise adding the organic phase into the water phase under a stirring condition, stirring, performing ultrasonic treatment, washing, and drying to obtain the retinol retinoate-ceramide liposome. Performing membrane extrusion to obtain a liposome suspension; the retinol retinoate-ceramide liposome modified by hyaluronic acid and salts thereof prepared by the invention has excellent stability, skin transmittance and anti-aging and moisturizing effects, also has low irritation, and is suitable for anti-aging cosmetics.

The utility model discloses a cell lipidomics detection sampling device which comprises a test tube assembly, a cell lipidomics detection sampling device and a cell lipidomics detection sampling device, the suction tube assembly comprises a suction tube main body, a glass rod, a piston and a holding rod; the middle of the test tube body is movably connected with the straw body, the middle of the straw body is movably connected with the glass rod, the piston is fixedly mounted at the bottom of the glass rod, and the holding rod is fixedly mounted at the top of the glass rod; the device further comprises a fixed density assembly. The fixed density assembly comprises a threaded cylinder and a screw column; the top of the test tube main body is fixedly provided with a threaded cylinder, and the outer ring of the test tube main body is fixedly provided with a screw column which is connected with the threaded cylinder. The utility model relates to a cell lipidosome sampling device, which solves the problem that cell lipidosome liquid in a test tube is easy to contact with the outside and the cell lipidosome is easy to pollute because a rubber head dropper is directly inserted into a test tube for sampling in the existing cell lipidosome and a gap is reserved between the rubber head dropper and the test tube in the process.

The invention relates to a high-coverage quantitative analysis method of lipidome and a quantitative kit, the method adopts a mobile phase A and a mobile phase B with different pH values for elution, the pH value of the mobile phase B is 2.5-5, and the pH value of the mobile phase A is 2.5-3.0. According to the method, good separation of acid-sensitive lipid compounds such as PA, S1P and PS can be realized, ionization inhibition of lipid compounds such as free fatty acid and free cholesterol can be reduced, and the quantitative sensitivity of the lipid compounds can be improved. According to the method, 104 lipid subclasses can be covered, 2375 lipid compounds can be quantified, and the number of the lipid compounds is more than two times that of the lipid compounds in the existing lipidomics quantitative method. The lipidome quantitative kit can be used for quantitatively analyzing lipidomes of seven biological samples such as plasma, urine, cells, liver and excrement of human or animal sources as well as escherichia coli and arabidopsis rosette leaves, and can meet quantitative analysis requirements of lipidomes in different biological samples.

The invention discloses an aptamer-modified lipid nano delivery platform as well as a preparation method and application thereof, and belongs to the field of biomedicine. The platform is prepared by taking DPPC, DOTAP and cholesterol as basic lipid components, Ce6 as a sound-sensitive agent and Flt3L as an immune agonist, controlling the particle size through gradient extrusion, and coupling cholesterol with an EpCAM aptamer for surface modification. The method has the core advantages that active targeting enrichment of tumors is realized by virtue of the aptamer, tumor cell immunogen cell death (ICD) is induced in combination with a sonodynamic therapy (SDT), and damage-related molecular patterns (DAMPs) are released; meanwhile, Flt3L is released in a tumor microenvironment, collection and activation of type 1 classical dendritic cells (cDC1) are specifically promoted, an'endogenous cDC1 vaccine 'is constructed, and CD8 + T cell mediated anti-tumor immune response is enhanced. Experiments prove that the platform can significantly improve the cDC1 infiltration level of a tumor site, effectively inhibit the progress of prostate cancer (PCa), and provide a new normal form for immune'cold tumor 'treatment.

The invention relates to a material for local drug delivery and an application thereof. In particular, provided are nanoparticle compositions comprising a lipid component comprising a compound of formula (I). The nanoparticle compositions of the invention are delivered only at the site of administration.

Provided herein are methods and materials for using organisms (e.g., engineered yeast strain-derived) to generate lipid compositions which can modulate cellular aging, extend lifespan, and / or modulate gut homeostasis in a subject. The lipid compositions contain at least twelve of the lipids selected from a phosphatidylethanolamine (PE); a lysophosphatidylethanolamine (LPE); a diacylglycerol (DG); a monoglyceride (MG); an acyl carnitine (AcCa); a phosphatidic acid (PA); a phosphatidylglycerol (PG); a lysophosphatidylcholine (LPC); a triacylglycerol (TG); a cholesterol ester (ChE); a wax ester (WE); a phosphatidylserine (PS); a phosphatidylcholine (PC); a phosphatidylinositol (PI); a ceramide (Cer); and a lysophosphatidylserine (LPS).

A method of increasing lipid synthesis or accumulation in and optionally secretion from a mammary epithelial cell (MEC) culture is provided. The method contacts the MEC with an LXR agonist in the presence of a fatty acid to increase lipid synthesis or accumulation and optionally increase lipid secretion. Also provided is a method of producing synthetic fat globules, the method comprising: (a) isolating lipid droplets from cultured cells; (b) encapsulating the droplets with a lipid bilayer, thereby producing synthetic fat globules. Compositions thus produced are also provided.

Disclosed herein are aspects of a compound according to Formula (I) Y[-Z-Ao-X]y. The compound may have a structure according to Formula (II) or Formula (III). Also disclosed are compositions comprising the compounds that may be useful for delivering agents such as therapeutic and / or prophylactic agents, for example, nucleic acids such as, but not limited to, DNA or RNA, small molecules, proteins, polypeptides or peptides. In some aspects, the composition is a lipid nanoparticle. Also disclosed herein are lipid nanoparticles comprising the compounds and methods for making and using the nanoparticles.

The invention relates to drug delivery materials and uses thereof. In particular, provided are nanoparticle compositions comprising a lipid component comprising a compound of formula (I). The nanoparticle compositions of the invention are delivered only at the site of administration.

Lipid composition comprising: (i) one or more cationic lipids, preferably cationically ionizable lipids, (ii) one or more phospholipids, and (iii) one or more statistical copolymers comprising repeating units of the following formula (I-a) and repeating units of formula (II-a) wherein R1 represents a hydrogen atom or a group selected from a C1-3 alkyl group, a cyclopropyl group, and a C3 alkenyl group; R2 represents a group selected from a saturated C4-40 hydrocarbyl group; an unsaturated C4-40 hydrocarbyl group having one or more carbon-carbon double and / or triple bonds; a saturated C4-40 heterohydrocarbyl group containing 1 to 5 divalent groups selected from ether, thioether, sulfone, sulfoxide, keto, ester, amide, carbamate and carbonate groups, and / or 1 to 5 fluorine atoms as substituents; and an unsaturated C4-40 heterohydrocarbyl group having one or more carbon-carbon double and / or triple bonds, and containing 1 to 5 divalent groups selected from ether, keto, ester, and carbonate groups and / or 1 to 5 fluorine atoms as substituents; and x and y independently represent 0 or 1.

The invention relates to the field of tumor immunotherapy, in particular to an mRNA vaccine targeting fusobacterium nucleatum membrane protein FomA and application of the mRNA vaccine in esophageal squamous cell carcinoma immunotherapy. The vaccine comprises a nucleotide sequence for coding a specific epitope of FomA, and the specific epitope contains 1-10'GGSGGGGSGG 'repetitive sequences and has homology gt in different strains; 95%. The mRNA is encapsulated in lipid nanoparticles (the particle size is 80-150 nm, the polydispersity coefficient is lt; and the liposome is composed of 38-42% of cationic lipid, 18-22% of neutral lipid, 33-37% of cholesterol and 3-5% of PEG modified lipid, and the encapsulation efficiency is 85-95%. The vaccine can specifically reduce the abundance of fusobacterium in tumors by 72.4 + / -8.0%, the tumor microenvironment is converted into an immune activation type, and the proportion of CD8 + T cells is increased from 5.2 + / -1.0% to 14.5 + / -2.0%. When the compound is combined with a PD-1 inhibitor, the objective remission rate reaches 70%, the tumor volume is reduced by 78.5 + / -8.0%, and the compound is suitable for treating clostridium-enriched esophageal squamous carcinoma.