392 results about "Pseudomonas" patented technology

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The invention relates to a multifunctional pseudomonas Y2-2 and application thereof, the strain is a multifunctional microbial strain, can effectively utilize microorganisms to solve the problem of heavy metal environmental pollution remediation, can also restrain various plant diseases and insect pests, and is classified and named as Pseudomonas sp. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 21, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34287. The method can effectively utilize microorganisms to repair heavy metal environmental pollution, can inhibit various plant diseases and insect pests, is good in effect and high in efficiency, and has practical popularization and application values.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The present invention relates to a (Rhodopseudomonas telluris DAEJI-NZ23 strain with accession number KACC81302BP, which exhibits nitrogen fixation capability, produces indole acetic acid (IAA), and has activities of reducing greenhouse gas, promoting plant growth, and secreting extracellular enzymes, and uses thereof. The strain of the present invention can be advantageously utilized in the technical field of greenhouse gas reduction, and is expected to increase crop yield while reducing excessive use of agricultural chemicals.

The invention discloses a pseudomonas capable of efficiently and rapidly degrading chloramphenicol and application of the pseudomonas. The name of the strain is Pseudomonas sp. MH-W1, the preservation number of the strain is GDMCC No: 66907, and the strain is preserved in the Guangdong Microbial Culture Collection Center on September 1, 2025. The strain can tolerate 200 mg / L of chloramphenicol; 100 mg / L of chloramphenicol can be degraded within 8 h, and the degradation rate reaches about 80%. According to the method provided by the invention, the degradation time can be remarkably shortened, the degradation efficiency is improved, the degradation condition is mild, the operation is simple and convenient, large-scale application is easy to realize, and the method has a wide industrial popularization prospect. The strain is an aerobic bacterium, is fast in growth and reproduction, simple to prepare, low in cost, convenient to use and high in efficiency, and can be used for repairing the environment polluted by chloramphenicol.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

This invention discloses a halophilic fast-growing bacillus strain that degrades chlorinated persistent organic pollutants and its applications. The strain is *Celeribacter baekdonensis* TBC005, with accession number GDMCC No:65236. The *Celeribacter baekdonensis* TBC005 of this invention exhibits excellent degradation ability against chlorinated persistent organic pollutants. After 14 days of anaerobic culture, it shows significant degradation of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP reached 21.5%, 16.7%, and 16.9%, respectively; after 35 days of cultivation, the degradation rates of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP can reach 50.7%, 59.2%, and 61.3%, respectively, and they can be applied to the environmental pollution control and bioremediation of chlorinated persistent organic pollutants.

The application discloses a high-salt-resistant denitrifying bacterial strain and a microsphere preparation thereof, a preparation method and application thereof, relates to the technical field of environmental microorganisms and wastewater biological treatment. Pseudomonas sp. The classification name of the strain is Pseudomonas sp., the preservation number is CGMCC No. 36626, the strain is preserved in the China General Microbiological Culture Collection Center, and the preservation date is November 14, 2025. The preparation method of the microsphere preparation containing the strain comprises the following steps: carrying out strain amplification culture to obtain a bacterial liquid, mixing the bacterial liquid with porous starch and a protective agent, then performing vacuum freeze drying to obtain bacterial powder, and then mixing the bacterial powder with a sodium alginate solution, and dropping the mixture into a calcium chloride solution to obtain the microsphere preparation. The microsphere preparation can maintain a nitrate nitrogen removal rate of more than 80% after being stored at-18 to-22 DEG C or 20 to 25 DEG C for 28 days, and can be used for denitrification and nitrogen removal of low-carbon-nitrogen-ratio and high-salt wastewater (such as urine wastewater in a closed circulation system).

A phage composition and an use thereof in strengthening soil carbon fixation, said phage composition including three phages, namely, a polyvalent phage φYSZKP that attacks Klebsiella pneumoniae and Pseudomonas aeruginosa; a Bacillus cereus phage φYSZBA1; and a Bacillus cereus phage φYSZBA2. In the present invention, the phage composition is injected into the target soil, and an approach of strengthening soil carbon fixation by means of cooperation with the host bacteria through injection of AMGs from the phage is employed; at the same time, the phage has a function of regulating the soil microbial communities from bottom to top; a network of symbiosis with indigenous bacteria is further optimized, and the soil carbon fixation potential is improved. This technique is an environmental improvement technique that achieves a good carbon fixation effect, has a low price and is environment-friendly.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The invention discloses a high-density fermentation method and application of an azotobacter strain, and belongs to the technical field of microorganisms. The invention discloses a high-density fermentation method of an azotobacter strain, which comprises the following steps: preparing a seed solution of rhodopseudomonas palustris Wrhzom-001, and inoculating the seed solution into tap water or underground water for high-density fermentation. The invention effectively solves the problems of low strain cell density, high infectious microbe content and unstable active amino acid metabolite in the high-density fermentation process of rhodopseudomonas palustris under the conditions of non-sterilization in the field and changeable temperature and air temperature.

The present application belongs to the technical field of biological medicine, and particularly relates to a thiol phenylboronic acid mediated nano-enzyme immunochromatography reagent and application thereof. The present application utilizes the broad-spectrum binding ability of MPBA to the glycosylated molecules on the surface of pathogens, and combines the colorimetric catalytic double enhancement effect of the virus-like biomimetic magnetic nano-enzyme (FeAu@AuIr), to improve the detection sensitivity. The probe takes Fe3O4 magnetic nanoparticles as the core, and sequentially loads large-diameter gold nanoparticles (Au) and gold-iridium alloy (AuIr) with high catalytic activity, to enhance the pathogen capture capacity and signal amplification effect. Combined with the antibody modified immunochromatography strip, Streptococcus pneumoniae, Pseudomonas aeruginosa and SARS-CoV-2 can be simultaneously detected, and the detection sensitivity is greatly improved. Through verification of 170 clinical samples, the present application has good application prospect in on-site rapid detection, and provides an efficient and low-cost solution for precise screening of respiratory tract infectious pathogens.

The invention relates to the technical field of microorganisms, and discloses efficient detoxification of a strain of pseudoxanthomonas to ochratoxin A and application of the strain of pseudoxanthomonas to the ochratoxin A. A strain of OTA efficient detoxification strain pseudoxanthomonas X-22 is screened out, and the pseudoxanthomonas X-22 can reduce 50 mu g / mL of OTA (10000 times of the national standard) to 0 within 70 min. The strain is mainly used for performing biological detoxification on OTA through intracellular enzyme, and a detoxification product is OTalpha with extremely low toxicity. The optimal growth conditions of the pseudoxanthomonas X-22 are as follows: 1.5 wt% of glucose is added into a basic culture medium as a carbon source, 1.5 wt% of yeast extract is added into the basic culture medium as a nitrogen source, the pH value of the culture medium is adjusted to 7.5, and the culture temperature is 37 DEG C. The OTA detoxification strain pseudoxanthomonas X-22 is applied to fermentation detoxification of polluted corn and oat, the detoxification rates of the corn and the oat are 61.29% and 97.26% respectively, OTA detoxification strain resources are enriched, and a theoretical basis is provided for application of a biological detoxification technology in the corn and the oat.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The present invention relates to an antibacterial or antifungal composition and a pharmaceutical composition for preventing or treating bacterial or fungal infections, both comprising VLX600. The antibacterial composition comprising VLX600 according to one aspect of the present invention exhibits antibacterial or antifungal activity against a wide range of strains, including Mycobacterium spp., Escherichia spp., Pseudomonas spp., Staphylococcus spp., Acinetobacter spp., Candida spp., and the like. The pharmaceutical composition comprising VLX600 demonstrates excellent antibacterial, antifungal, and therapeutic efficacy against pathogens and can be effectively utilized for the prevention or treatment of bacterial or fungal infections. Furthermore, VLX600 exhibits a synergistic effect with conventional antibiotics and thus is effective in treating infections caused by antibiotic-resistant bacterial strains.

To provide a new agent for controlling soil-borne plant diseases, containing a microorganism as an active ingredient.SOLUTION: An agent for controlling a soil-borne plant disease, comprising, as an active ingredient, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 96% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 1, a Pseudomonad bacterium having a 16SrRNA genome comprising a nucleotide sequence having 98% or more sequence identity to a nucleotide sequence represented by SEQ ID NO: 2, or a combination thereof.SELECTED DRAWING: None

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The invention belongs to the field of microorganism application, and particularly relates to a bacterium capable of degrading pyrethroid pesticides, and a preparation and application thereof. The invention relates to the field of biotechnology, and in particular relates to a pseudomonas aeruginosa, which is named as paracoccus panotrophus BB-2 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251184, and pseudomonas aeruginosa, which is named as pseudomonas aeruginosa BS-3 and is preserved in the China Center for Type Culture Collection on May 26, 2025 with the preservation number of CCTCC NO: M 20251185. The invention further relates to a mixed bacterial agent of the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3, it is found that the paracoccus pantotrophus BB-2 and the pseudomonas aeruginosa BS-3 can be subjected to synergistic symbiosis and secrete and produce extracellular hydrolase and multiple oxidoreductases, pyrethroids are efficiently degraded, and the removal efficiency of the multiple pyrethroids reaches 90% or above.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The invention discloses a strain of pseudomonas gestacei LAB-SA 50, which is classified and named as pseudomonas gestacei, the preservation date is September 18, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35979. The strain of pseudomonas gestacei LAB-SA 50 is named as pseudomonas gestacei LAB-SA 50. The invention also discloses a microbial inoculum prepared from the strain. The invention further discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in growth promotion of saline-alkali soil crops, and the saline-alkali soil is soda type saline-alkali soil. The screened pseudomonas georgi LAB-SA 50 has relatively strong salt resistance and alkali resistance, and can colonize and stably exert functions in a soda type saline-alkali stress environment, so that the damage of soda type saline-alkali stress to crops (such as corn) is effectively relieved, and the growth of the crops (such as corn) is promoted.

The invention provides a pseudomonas aeruginosa engineering bacterium which is based on a PAO1 wild type strain, and two of the following genes, namely cyaA and cyaB, are knocked out from a genome at the same time. According to the pseudomonas aeruginosa engineering bacterium, the cyaA gene and the cyaB gene are knocked out at the same time, and the yield of rhamnolipid is remarkably increased on the premise that existing PAO1 fermentation process parameters and processes are not changed. The yield cannot be increased when the cyaA or the cyaB is singly knocked out, and the synergistic effect of double-gene knockout is proved.

The invention discloses a nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1) with a capability of degrading multiple pollutants and an application of the nicosulfuron pseudomonas (Pseudomonas nisulfuron HD1, and belongs to the technical field of screening and application of functional microorganisms. The strain is derived from a chicken manure aerobic composting environment, the preservation number is GDMCC No: 67351, and nitro polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons and tetracycline antibiotics can be efficiently degraded at the same time. In a single pollutant with the concentration of 50 mg / L, the degradation rates of tetracycline and doxycycline exceed 97% in 8 h, the degradation rate of nitrofluoranthene reaches 99.01% in 48 h, and the degradation rate of phenanthrene reaches 66.17%. In nitrofluoranthene-phenanthrene composite pollutants, the 48-hour degradation rate is kept to be 78% or above. The strain is high in degradation efficiency and strong in environmental adaptability, provides a new microbial resource for bioremediation of a combined pollution site, and has a wide application prospect.

This invention discloses the application of arginine-depleted engineered bacteria for the treatment of soft tissue sarcomas, including preliminary validation, obtaining qualified engineered bacteria SGR1, obtaining drug compositions and regimens, experimental conclusions, and clinical application promotion. During use, clinical soft tissue sarcoma samples are collected, including undifferentiated pleomorphic sarcoma, synovial sarcoma, and liposarcoma. Immunohistochemistry (IHC) is used to detect ASS1 protein expression, qPCR is used to detect ASS1 gene transcription levels, and the proportion of ASS1 deletion / low expression is statistically analyzed. Arginine auxotrophic sarcoma subpopulations are confirmed based on literature. Cells are cultured in arginine-containing or arginine-free media, and cell viability, colony formation, cell cycle, and apoptosis are detected. The sensitivity of ASS1-deficient cells to arginine deprivation is verified, and therapeutic targets are identified. The enzyme activity, substrate specificity, and immunogenicity of human ARG I / II, mycoplasma ADI, and Pseudomonas ADI are compared. High-activity, low-immunogenic enzymes or enzyme combinations (such as human ARG I + microbial ADI) are selected, and site-directed mutagenesis or codon optimization is performed on microbial ADI to increase expression levels.