1546 results about "Pseudomonas" patented technology

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

Methods and compositions relating to recombinant anti-CD22 antibodies with high binding affinity, and immunoconjugates comprising the anti-CD22 antibody linked to a therapeutic agent such as a Pseudomonas exotoxin or a detectable label. The invention provides diagnostic methods, and means to inhibit the growth of malignant B cells.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

A heterologous expression in a host Pseudomonas bacteria of an optimized polynucleotide sequence encoding a protein.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The present invention provides a microbe composition, tobacco-dedicated biological organic fertilizer containing the composition, its usage method. The microbe composition comprises aztobacter sp., pseudomonas fluorescence, Bacillus megaterium, bacillus mucilaginosus and bacteria capable of preventing and treating plant disease and insect pests. The microbe composition is mixed according to proportion with oligochitosan, lignite, grass charcoal, and bean cake and fermented to make bacteria powder fertilizer, and further added with potassium-enriching shale, bentonite and water-keeping agent to make bacteria granule fertilizer. The tobacco-dedicated biological organic fertilizer contains the above-mentioned bacteria powder fertilizer and granule fertilizer which contains natural mineral and good organic substance, C/N value being large, which fit the growth rule of tobacco plant and promote potassium fertilizer efficiency and possesses long-lasting potassium-supply capability and provides effective ingredient for tobacco.

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

An extremoyzme over-expression system in which Pseudomonads and closely related bacteria are used as host cells, and methods and kits for use thereof, extremozymes expressed therefrom.

The invention relates to a method of restoring plants that are polluted by heavy metal with little hairy euphorbia or interplant of the little hairy euphorbia and ramie planted in the heavy metal polluted field, or inoculating heavy metal activated bacteria that are bacillocin or pseudomonas after planting the above-mentioned plants. With above-mentioned plant which absorbs heavy mental and the activated bacteria that breaks heavy metal away from the plant 's root and activates them, the invention makes the heavy metal polluted field restored quickly and effectively.

The invention provides a Pseudomonas exotoxin A (PE) comprising an amino acid sequence having a substitution of one or more B-cell and/or T-cell epitopes. The invention further provides related chimeric molecules, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions. Methods of treating or preventing cancer in a mammal, methods of inhibiting the growth of a target cell, methods of producing the PE, and methods of producing the chimeric molecule are further provided by the invention.

The invention relates to an organic materials zymolysis complex bacillus agent in the field of zymolysis bacillus agent technology. It comprises: low temperature bacteria, Bacillus cereus, gliotoxin, pseudomonas, slim streptomycete, high temperature bacteria and high temperature actinomyces with the weight ration 4:2:1:1:3:2:2. The low temperature bacterium is enzyme yeast with the heat resisting temperature below 20 deg. The high temperature bacterium is enzyme yeast with the heat resisting temperature below 70 deg. The heat resisting temperature below 70 deg of the high temperature actinomyces bellows 70 deg.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention discloses bacillus amyloliquefaciens JK6 and biological fertilizer and application. The serial number of strain preservation is CGMCC No. 10658 and is stored in the China general microbiological culture collection center on March 23, 2015. The bacillus amyloliquefaciens JK6 is mainly used for biological prevention and control of pseudomonas solanacearum and is provided with an iron-production carrier, a biological membrane, protease, catalase, cellulose and IAA characteristics, multiple fungal pathogens of banana wilt pathogen, litchi anthrax germs, litchi frost phytophthora pathogens, rice blast fungus, cucumber wilt pathogen and the like, and growth of lettuce plants is obviously promoted; the K6 strain can be successfully planted, and disease preventing functional genes of yndj, srfAB, fend and ituC are expressed in soil; the strain is high in reproduction speed, simple in production technology, high in adverse resistance, easy to store and favorable for industrialized production, the biological fertilizer is prepared with the strain, soil-born diseases can be prevented and controlled, growth of the plants can be promoted, and application prospect is broad.

The invention relates to microcapsules prepared from viable microbes such as pseudomonas, bacillus and paenibacillus serving as main active components, belonging to the technical field of microorganism and biochemistry. The method for preparing microcapsules of viable microbe biopesticide comprises the following steps of: adsorbing microbial fermentation culture solution through an adsorption effect to prepare capsule cores and solidifying the capsule cores, and adding wall materials, so as to prepare a microcapsule preparation. The microcapsule preparation prepared by adopting the method has high content of viable microbes, high storage property and strong high-temperature and ultraviolent resistances, and stable and good prevention and control effect on plant diseases and insect pests.

The invention relates to a microbial agent for purifying river wastewater and a preparation method of the microbial agent. The microbial agent comprises the following components in parts by weight: 20-40 parts of bacillus natto, 10-30 parts of nitrobacteria, 5-15 parts of white-rot fungi, 5-15 parts of pseudomonas and 10-40 parts of photosynthetic bacteria. The microbial agent is well-adapted and has the capabilities of fast forming dominant bacterial communities and replenishing indigenous rhizobia to the water body; and the microbial agent can be used for reducing the COD (Chemical Oxygen Demand), NH3-H, total nitrogen, TP (Total Phosphorus) and the like, removing odor and color and producing high-activity bio-enzyme to suppress the growth of harmful bacteria and acceleratively decompose residual baits as well as animal and plant residues, and a remarkable water quality purifying effect is achieved.

The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.

The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.

The invention relates to a preparation method for a compound fungus agent for specifically degrading an organic matter for a biological enhancing water treatment system. The compound fungus agent is added into a biological treatment process, has high biological activity, a stable effect and low cost, is easy to adsorb on the surface of a carrier and is difficult to lose. The preparation method issimple and practical, and has a short preparation period and low cost. The compound fungus agent comprises the following four strains: 20 to 30 percent of pseudomonas-stutzeri, 20 to 30 percent of pseudomonas putida, 10 to 20 percent of pseudomonas-pertucinogena and 30 to 50 percent of bacillus subtilis. The compound fungus agent is prepared by combining and screening the strains, performing slant culture and primary culture, mixing according to the proportion, and performing secondary cycle culture. The preparation method is widely used in a biological enhancing water treatment process, has a long-term and stable operation period, and has specific degradation ability for toxic and harmful organic pollutants such as halogenated hydrocarbons, phenol, benzoic acid, aromatics, polycyclic aromatic hydrocarbon, heterocyclic compounds, phthalic acid ester and the like.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The present invention is directed to methods for delaying a plant development process comprising exposing a plant or plant part to one or more bacteria or enzymes. In specific embodiments, the one or more bacteria are selected from the group consisting of Rhodococcus spp., Pseudomonas chloroaphis, Brevibacterium ketoglutamicum, and a mixture comprising any combination of these bacteria. Apparatuses for delaying a plant development process comprising a catalyst that comprises one or more of the above bacteria.

The invention provides novel recombinant immunotoxins comprising domain III of cholix toxin and exotoxin from Vibrio cholerae. The present invention further provides methods for using the compositions of the present invention to (i) induce apoptosis in a cell bearing one or more surface markers (ii) inhibit unwanted growth, hyperproliferation or survival of a cell bearing one or more cell surface markers, (iii) treat a condition, such as a cancer, (iv) provide therapy for a mammal having developed antibodies to Pseudomonas exotoxin A, and (v) provide therapy for a mammal having developed a disease caused by the presence of cells bearing one or more cell surface marker.