1115 results about "Pseudomonas" patented technology

The invention relates to the technical field of antibacterial peptides, and particularly discloses a marine antibacterial peptide and application thereof, and the amino acid sequence of the marine antibacterial peptide is as shown in SEQ ID NO.1: MWKLPKWRNGRRRGFKIPRW. The marine antibacterial peptide CD109 provided by the invention has an inhibition effect on staphylococcus aureus, acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae, and has extremely low cytotoxicity.

The invention relates to the technical field of microorganisms, and provides a low-temperature-resistant heterotrophic nitrification-aerobic denitrification pseudomonas and application thereof, the strain is pseudomonas (Pseudomonas sp.) SW1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20221797. The low-temperature-resistant heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. SW1 has the characteristic of low temperature resistance, can well grow at 4-10 DEG C, and has relatively strong heterotrophic nitrification-aerobic denitrification nitrogen removal capability, and the enrichment culture of the strain is carried out in a relatively low nitrogen source, and the condition is extremely consistent with the application scene of the strain in domestic sewage.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention discloses low-temperature-resistant and high-salt-resistant aerobic pseudomonas kirschner kirschner K2 as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out low-temperature and high-salt enrichment culture; screening and domesticating, and monitoring the concentration of ammonia nitrogen; and after 3-5 times of enrichment culture, diluting the enrichment culture, further culturing until a macroscopic bacterial colony is obtained, and then picking and carrying out plate streaking to separate a pure strain. The invention also relates to application of the strain in preparation or prevention of nitrification and denitrification nitrogen removal drugs in treatment of high-salt nitrogen-containing wastewater. The strain is low-temperature-resistant and high-salt-resistant heterotrophic-aerobic denitrifying bacteria, has the characteristic of low temperature resistance, grows well at low temperature and high salt salinity, has higher heterotrophic nitrification-aerobic denitrification nitrogen removal capacity, is suitable for nitrogen removal treatment of high-salt wastewater such as food processing wastewater and landfill leachate, and has wide application prospects. The superiority is particularly obvious under a low-temperature condition.

The invention discloses a microbial compound fertilizer for improving soil pollution, which is prepared by mixing a matrix fertilizer, alginic acid, cellulase, modified bentonite, biochar, a microbial agent, nano zero-valent iron and monopotassium phosphate according to a mass ratio of (30-40): (6-10): (5-8): (20-25): (15-20): (3-5): (4-6): (4-8), the microbial agent comprises pseudomonas, bacillus and silicate bacteria; heavy metal ions in soil can be effectively neutralized through the nanoscale zero-valent iron and the phosphate, pollutants in the soil can be effectively degraded through the microbial flora, meanwhile, nutrients needed by plant growth are provided, the modified bentonite is added, the soil structure can be changed, meanwhile, phosphorus and potassium fertilizers are provided for the soil, and the soil quality is improved. By adding alginic acid and cellulase, the activity of microorganisms is effectively enhanced, the fertility conversion efficiency of the microorganisms is improved, and soil improvement and remediation are achieved.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention discloses a mud flat-derived malathion degrading bacterium and application thereof, and relates to the technical field of biology. The malathion degrading bacterium is Ectopseudomonas khazarica FJ5-34-2, and is preserved in the China General Microbiological Culture Collection Center on November 8, 2024, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.32547. The malathion degrading bacterium has the advantages that the malathion degrading bacterium can be used for degrading malathion; according to the invention, an in-vitro pseudomonas strain FJ5-34-2 is screened from a mud flat sample in a new area of Northern Daihe River in Qinhuangdao, Hebei province. The in-vitro pseudomonas can effectively degrade malathion, and the degradation rate of 100 mg / L malathion within 72 hours can reach 94.93%. The invention provides a new effective microorganism for microbial degradation of malathion, which has great practical significance for relieving environmental residues of pesticides.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention discloses a complex microbial inoculant and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent comprises bacillus (bacillus sp.BS-K1) and pseudomonas sp.CN-B1), and the preservation number of the bacillus is CCTCC (China Center for Type Culture Collection) NO: M 20242022, and the preservation number of the pseudomonas is CCTCC NO: M 20242022. The preservation number of the pseudomonas is CCTCC (China Center for Type Culture Collection) NO: M 20242021. According to the complex microbial inoculant provided by the invention, probiotics (Bacillus sp.BS-K1) and denitrifying bacteria (Pseudomonas sp.CN-B1) which have antibacterial effects are compounded to obtain the complex microbial inoculant for aquaculture, and when the complex microbial inoculant is applied to an aquaculture water body, the complex microbial inoculant has excellent denitrification capability and an inhibition effect on related pathogenic bacteria in the water body; the removal efficiency of ammonia nitrogen and nitrite nitrogen in the culture water body can be improved, the number of related pathogenic bacteria is reduced, and the safety of the culture environment is guaranteed.

The invention discloses pseudomonas TB392 and application thereof in degradation of pectin and generation of flavor substances in tobacco leaves, and belongs to the technical field of biology. The strain is preserved in the China General Microbiological Culture Collection Center on October 16, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32230. The strain can produce pectinase at high yield, has the capability of reducing nicotine, can degrade macromolecular compounds such as carotenoid, starch, pectin, cellulose and the like in tobacco leaves, and can remarkably improve the content of flavor substances in the tobacco leaves. After the strain or the prepared microbial agent is applied to tobacco leaves, the quality of the tobacco leaves can be improved, and the use value of the tobacco leaves is improved.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

A method of production of granular soil conditioner based on a mixture of biochar and organic fertilizer comprising the following steps:Biochar mixing with animal excrements in a ratio of 1 part of biochar to 0.1 to 0.4 parts of the excrement dry matter, adding to the base of a mixture of soil bacteria belonging to at least two genera of the following: bacteria of the Rhizobia genus, nitrifying bacteria of the Azospirillum or Azotobacter genus, bacteria of the Pseudomonas genus, and bacteria of the Bacillus genus,topping the bacteria-endowed base with water in the weight not more than double the weight of the bacteria-endowed base and letting the mixture ferment for at least 5 days,draining off the excess water and drying the bacterial base so that the water content does not exceed 20% by weight,adding a mixture of spores of fungi containing at least two of the group including Trichoderma, Arbuscular mycorrhizal fungi, Ectomycorrhizal fungi to the base-bacteria compound in a total quantity corresponding to 0.2 to 1% of the dry matter weight of the base-bacteria compound.

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention relates to the technical field of new strains, and provides a new strain which is screened from Xinjiang saline-alkali soil, is classified and named as sphingomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.32220. Research finds that the strain can remarkably reduce the soil conductivity, the exchangeable sodium content, the alkalization degree and the total salt content, so that the soil quality is improved. The organic matter content and the acid phosphatase activity of the soil are remarkably improved, and the quality of the saline-alkali soil can be efficiently improved. Meanwhile, the invention provides a compound bacterial fertilizer for effectively improving saline-alkali soil, the compound bacterial fertilizer comprises bacillus subtilis, Exiguobacterium acetylbacter, Bacillus velezensis, Pseudomonas fluorescens, Rhodococcus capsulatus and a new strain Sphingomonas sp., and research finds that compared with a single bacterial solution, the compound bacterial solution has a more prominent remediation effect on saline-alkali soil.

The invention discloses lactobacillus plantarum and application thereof, and aims at solving the technical problems of how to inhibit the growth of malassezia furfur, splitting the malassezia furfur, and inhibiting the growth of pseudomonas aeruginosa and staphylococcus aureus and the formation of a biological membrane. In particular discloses lactobacillus plantarum, the lactobacillus plantarum is lactobacillus plantarum DZ041, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.20835 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Bacterial suspension or cell-free fermentation liquor prepared from the malassezia furfur can inhibit the growth of malassezia furfur and even can crack malassezia furfur, and the malassezia furfur can be used for industrial production.

The invention discloses a pseudomonas for antimony detoxification and autotrophic nitrogen fixation and application of the pseudomonas. The strain is separated from the rhizosphere of a pioneer plant in antimony-polluted soil of a tin mine in cold water river city, Hunan province, and is identified to be capable of quickly oxidizing high-toxicity antimony (III) into low-toxicity antimony (V) through autotrophic metabolism in a high-antimony environment, so that antimony detoxification is realized, and the detoxification rate is 108.6 (mu mol / (L.day)). The strain has autotrophic nitrogen fixation activity and can achieve autotrophic growth under the oligotrophic condition of a mining area, and the nitrogenase activity is larger than or equal to 223.4 (nmol C2H2 / (g.h)). Particularly, the antimony detoxification and nitrogen fixation capabilities of the strain have a synergistic effect, so that the strain has unique advantages in the field of heavy metal antimony pollution remediation, and dual effects of antimony pollution treatment and soil improvement can be synchronously realized; the development and application prospects are good.

The invention discloses Pseudomonas sp., a microbial agent prepared from the Pseudomonas sp. And application of the Pseudomonas sp. And the microbial agent prepared from the Pseudomonas sp. The pseudomonas strain N2-3-1-14 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation time is April 8, 2025; the preservation number is CGMCC 34128. The pseudomonas strain N2-3-1-14 and the microbial inoculum prepared from the pseudomonas strain N2-3-1-14 can effectively inhibit growth of pathogenic bacteria of pine blight and pine needle red spot, and the bacteriostasis rate is 65% or above. The strain can also significantly promote the growth of pinus sylvestris.

The invention discloses application of synbiotics to prevention and control of plutella xylostella pests. Probiotics in the synbiotics are a pseudomonas strain PPF1, the pseudomonas strain PPF1 is classified and named as Pseudomonas putida, and the prebiotic composition is ornithine, alanine, valine, serine, leucine and cycloleucine. The invention further discloses a preparation method of the prebiotic composition. Wherein the pseudomonas can improve the resistance of Chinese cabbage to plutella xylostella. According to the prebiotic composition, the resistance of Chinese cabbage to plutella xylostella is improved, meanwhile, the growth of the pseudomonas strain PPF1 is remarkably promoted, and the colonization ability of the pseudomonas strain PPF1 is improved. The synbiotics are applied into soil by a root irrigation method, so that the feeding area of the cabbage by the plutella xylostella can be remarkably reduced, and the pest of the plutella xylostella in the cabbage can be effectively prevented and controlled.

The invention discloses a phosphate solubilizing bacterium with eurytopic characteristic and application thereof, the bacterium is separated from corn straw biochar in which rice is continuously planted for 3 years, is identified as Pseudomonas paaversuta and is named as MPP2401, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuchang District, Wuhan, Hubei province, the preservation date is November 6, 2024, and the preservation number is CCTCC NO: M20242439. The strain can keep stable phosphate solubilizing capacity under the environmental conditions of 15-30 DEG C, pH of 7-10 and NaCl 0-0.8 mol.L <-1 >, and still has functional phosphate solubilizing capacity even under the condition of extreme low temperature of 5 DEG C, the composite stress of pH of 7-8 and NaCl 0-0.8 mol.L <-1 > and the composite stress of pH of 9-10 and NaCl 0-0.6 mol.L <-1 >. Meanwhile, the bacterium has the broad-spectrum antibacterial characteristic, and the antibacterial rate on fusarium graminearum, fusarium verticillioides and magnaporthe oryzae reaches 41.0-77.6%. Due to the unique wide environment adaptability and the phosphate solubilizing-bacteriostasis dual-function synergistic characteristic, a microbial germplasm resource with production-ecology dual values is provided for biochar bacterial fertilizer development, saline-alkali phosphorus activation and biological prevention and control.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention belongs to the field of microorganisms, and particularly discloses a Pseudomonas klackmussii strain TYQ5 and application thereof, the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27214, and the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The applicant finds that the Pseudomonas kirschner can secrete auxin, can promote the growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum, and also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum. Furthermore, the pseudomonas kirschner has the capability of degrading vegetable straws and the capability of solubilizing phosphorus, so that the applicant considers that the pseudomonas kirschner has a remarkable application prospect in the fields of agriculture and environmental protection.

The invention provides a selenium-rich apricot planting method, and belongs to the technical field of fruit tree planting. The planting method comprises the following steps: soil preparation and fertilization management; performing planting; fertilization: respectively carrying out topdressing before flowering, in a flower bud differentiation period and in a fruit expansion period; and harvesting. A fertilizer for topdressing is a selenium-rich fertilizer, and the selenium-rich fertilizer is composed of a microbial fertilizer, animal manure, waste plant rhizomes, monopotassium phosphate, urea and a substance containing a selenium element; the microbial fertilizer is prepared from saline-alkaline tolerant bacillus, pseudomonas and arbuscular mycorrhizal fungi. According to the selenium-rich fertilizer disclosed by the invention, the saline-alkaline tolerant bacillus, the pseudomonas and the arbuscular mycorrhizal fungi are added into the selenium-rich fertilizer, and the enrichment effect of apricot trees on selenium in soil is improved through the synergistic effect of the saline-alkaline tolerant bacillus, the pseudomonas and the arbuscular mycorrhizal fungi, so that the content of selenium in apricots is greatly increased.

The invention discloses a magnetic carbon-loaded slow-release microbial agent which comprises an activated carbon carrier containing ferroferric oxide nanoparticles and a compound microbial bacterium composed of bacillus megatherium, bacillus mucilaginosus, white-rot fungi and pseudomonas, and the mass ratio of the compound microbial bacterium to the activated carbon carrier is (3-4): 30. The invention further discloses a preparation method and application of the magnetic carbon-loaded slow-release microbial agent. According to the magnetic carbon-loaded slow-release microbial agent disclosed by the invention, the activated carbon containing the ferroferric oxide nano-particles is used as a carrier to load the compound bacteria, so that the stability of the microbial bacteria is improved, the microbial bacteria can better resist the impact of the environment, the service life of the microbial bacteria is prolonged, and the slow-release effect of the microbial agent in the remediation of vegetable planting soil is achieved; the nutrient absorption of the vegetables is promoted.

The invention discloses bacillus siamensis capable of antagonizing various phytopathogens and application of the bacillus siamensis. The preservation number of the bacillus siamensis A-2 is GDMCC (China General Microbiological Culture Collection Center) NO.64228. The bacillus siamensis A-2 has the advantages that the preservation number of the bacillus siamensis The strain has an obvious inhibition effect on Releasella syzygium jambos Indian niesia subspecies, pseudomonas solanacearum, fusarium solani, phomopsis, fusarium oxysporum, colletotrichum musae, colletotrichum laurosporum, fusarium fujikubin and fusarium sp. The gene clusters contain gene clusters for synthesizing plipastatin, abinogen, surfactin, siderophore, bacitracin, difixitin, butyrosidin A / B and some terpene compounds, and the gene clusters contain gene clusters for synthesizing plipastatin, abinogen, surfactin, siderophore, bacitracin, difixitin and butyrosidin A / B; the composition has a relatively good prevention and treatment effect (the relative prevention effect is 58.14%) on tomato bacterial wilt; and the strain has the capability of degrading tetracycline hydrochloride (the degradation rate in 3 days is 49.28%). Therefore, the bacillus siamensis A-2 has extremely high development and utilization value in agricultural production.

The invention belongs to the technical field of microorganisms, and particularly relates to a synthetic flora for promoting growth of macadamia nuts as well as a construction method and application of the synthetic flora. The invention relates to a synthetic flora. The synthetic flora is prepared by mixing bacillus subtilis X17, pseudomonas fluorescens X62 and trichoderma harzianum Z27 in equal volume. According to the invention, strains are separated and screened from healthy rhizosphere soil, and the strains which grow well are subjected to a pairwise confrontation test, so that the strains which have no antagonism in pairwise are screened out. According to molecular biology identification, three strains with the growth promoting effect, namely bacillus subtilis X17, pseudomonas fluorescens X62 and trichoderma harzianum Z27, are screened, then the three strains are constructed into a synthetic flora, pot experiment verification shows that the constructed synthetic flora can promote growth of macadimia nut plants and accumulation of biomass, meanwhile, soil can be activated, the yield of macadimia nut plants is increased, and the yield of macadimia nut plants is increased. The soil nutrient content, the enzyme activity and the microbial biomass carbon, nitrogen and phosphorus content are improved, and support can be provided for planting and economic development of macadamia nuts.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention discloses a bacterial strain and a method for producing pyrroloquinoline quinone. The strain is pseudomonas putida N1-1 which is preserved in the China General Microbiological Culture Collection Center on January 2, 2025, the preservation number is CGMCC NO.33266, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The strain is separated from myxomycete. The bacterial strain is inoculated into a fermentation culture medium, standing fermentation culture is carried out for 7 days at room temperature under the dark condition, a fermentation product is obtained and filtered, fermentation filtrate containing pyrroloquinoline quinine is obtained, and the yield of pyrroloquinoline quinine reaches 12.21 mg / L.

The invention relates to the technical field of organic fertilizers for saline-alkali soil, in particular to a semi-decomposed straw saline-alkali soil improvement fertilizer and application thereof. The straw semi-decomposed saline-alkali soil improvement fertilizer is prepared from the following raw materials in parts by weight: 50-70 parts of semi-decomposed straw, 3-7 parts of humic acid, 30-45 parts of farmyard manure and 2-3 parts of trace element fertilizer, wherein the semi-decomposed straws are obtained by mixing agricultural wastes and a microbial agent and then fermenting; the microbial agent is prepared by mixing a bacillus subtilis bacterial liquid, a pseudomonas bacterial liquid and a serratia bacterial liquid; the volume ratio of the bacillus subtilis liquid to the pseudomonas liquid to the serratia marcescens liquid is (2-3): (2-3): (2-4). The straw semi-decomposed saline-alkali soil improvement fertilizer prepared by the invention can effectively reduce the pH value of saline-alkali soil, and also can increase the yield of wheat in saline-alkali soil.