761 results about "Pseudomonas" patented technology

The invention relates to the technical field of microorganisms, and provides a low-temperature-resistant heterotrophic nitrification-aerobic denitrification pseudomonas and application thereof, the strain is pseudomonas (Pseudomonas sp.) SW1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20221797. The low-temperature-resistant heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. SW1 has the characteristic of low temperature resistance, can well grow at 4-10 DEG C, and has relatively strong heterotrophic nitrification-aerobic denitrification nitrogen removal capability, and the enrichment culture of the strain is carried out in a relatively low nitrogen source, and the condition is extremely consistent with the application scene of the strain in domestic sewage.

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention discloses low-temperature-resistant and high-salt-resistant aerobic pseudomonas kirschner kirschner K2 as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out low-temperature and high-salt enrichment culture; screening and domesticating, and monitoring the concentration of ammonia nitrogen; and after 3-5 times of enrichment culture, diluting the enrichment culture, further culturing until a macroscopic bacterial colony is obtained, and then picking and carrying out plate streaking to separate a pure strain. The invention also relates to application of the strain in preparation or prevention of nitrification and denitrification nitrogen removal drugs in treatment of high-salt nitrogen-containing wastewater. The strain is low-temperature-resistant and high-salt-resistant heterotrophic-aerobic denitrifying bacteria, has the characteristic of low temperature resistance, grows well at low temperature and high salt salinity, has higher heterotrophic nitrification-aerobic denitrification nitrogen removal capacity, is suitable for nitrogen removal treatment of high-salt wastewater such as food processing wastewater and landfill leachate, and has wide application prospects. The superiority is particularly obvious under a low-temperature condition.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

A method of production of granular soil conditioner based on a mixture of biochar and organic fertilizer comprising the following steps:Biochar mixing with animal excrements in a ratio of 1 part of biochar to 0.1 to 0.4 parts of the excrement dry matter, adding to the base of a mixture of soil bacteria belonging to at least two genera of the following: bacteria of the Rhizobia genus, nitrifying bacteria of the Azospirillum or Azotobacter genus, bacteria of the Pseudomonas genus, and bacteria of the Bacillus genus,topping the bacteria-endowed base with water in the weight not more than double the weight of the bacteria-endowed base and letting the mixture ferment for at least 5 days,draining off the excess water and drying the bacterial base so that the water content does not exceed 20% by weight,adding a mixture of spores of fungi containing at least two of the group including Trichoderma, Arbuscular mycorrhizal fungi, Ectomycorrhizal fungi to the base-bacteria compound in a total quantity corresponding to 0.2 to 1% of the dry matter weight of the base-bacteria compound.

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention discloses a pseudomonas for antimony detoxification and autotrophic nitrogen fixation and application of the pseudomonas. The strain is separated from the rhizosphere of a pioneer plant in antimony-polluted soil of a tin mine in cold water river city, Hunan province, and is identified to be capable of quickly oxidizing high-toxicity antimony (III) into low-toxicity antimony (V) through autotrophic metabolism in a high-antimony environment, so that antimony detoxification is realized, and the detoxification rate is 108.6 (mu mol / (L.day)). The strain has autotrophic nitrogen fixation activity and can achieve autotrophic growth under the oligotrophic condition of a mining area, and the nitrogenase activity is larger than or equal to 223.4 (nmol C2H2 / (g.h)). Particularly, the antimony detoxification and nitrogen fixation capabilities of the strain have a synergistic effect, so that the strain has unique advantages in the field of heavy metal antimony pollution remediation, and dual effects of antimony pollution treatment and soil improvement can be synchronously realized; the development and application prospects are good.

The invention discloses Pseudomonas sp., a microbial agent prepared from the Pseudomonas sp. And application of the Pseudomonas sp. And the microbial agent prepared from the Pseudomonas sp. The pseudomonas strain N2-3-1-14 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation time is April 8, 2025; the preservation number is CGMCC 34128. The pseudomonas strain N2-3-1-14 and the microbial inoculum prepared from the pseudomonas strain N2-3-1-14 can effectively inhibit growth of pathogenic bacteria of pine blight and pine needle red spot, and the bacteriostasis rate is 65% or above. The strain can also significantly promote the growth of pinus sylvestris.

The invention discloses a phosphate solubilizing bacterium with eurytopic characteristic and application thereof, the bacterium is separated from corn straw biochar in which rice is continuously planted for 3 years, is identified as Pseudomonas paaversuta and is named as MPP2401, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuchang District, Wuhan, Hubei province, the preservation date is November 6, 2024, and the preservation number is CCTCC NO: M20242439. The strain can keep stable phosphate solubilizing capacity under the environmental conditions of 15-30 DEG C, pH of 7-10 and NaCl 0-0.8 mol.L <-1 >, and still has functional phosphate solubilizing capacity even under the condition of extreme low temperature of 5 DEG C, the composite stress of pH of 7-8 and NaCl 0-0.8 mol.L <-1 > and the composite stress of pH of 9-10 and NaCl 0-0.6 mol.L <-1 >. Meanwhile, the bacterium has the broad-spectrum antibacterial characteristic, and the antibacterial rate on fusarium graminearum, fusarium verticillioides and magnaporthe oryzae reaches 41.0-77.6%. Due to the unique wide environment adaptability and the phosphate solubilizing-bacteriostasis dual-function synergistic characteristic, a microbial germplasm resource with production-ecology dual values is provided for biochar bacterial fertilizer development, saline-alkali phosphorus activation and biological prevention and control.

The invention belongs to the field of microorganisms, and particularly discloses a Pseudomonas klackmussii strain TYQ5 and application thereof, the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27214, and the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The applicant finds that the Pseudomonas kirschner can secrete auxin, can promote the growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum, and also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum. Furthermore, the pseudomonas kirschner has the capability of degrading vegetable straws and the capability of solubilizing phosphorus, so that the applicant considers that the pseudomonas kirschner has a remarkable application prospect in the fields of agriculture and environmental protection.

The invention discloses a magnetic carbon-loaded slow-release microbial agent which comprises an activated carbon carrier containing ferroferric oxide nanoparticles and a compound microbial bacterium composed of bacillus megatherium, bacillus mucilaginosus, white-rot fungi and pseudomonas, and the mass ratio of the compound microbial bacterium to the activated carbon carrier is (3-4): 30. The invention further discloses a preparation method and application of the magnetic carbon-loaded slow-release microbial agent. According to the magnetic carbon-loaded slow-release microbial agent disclosed by the invention, the activated carbon containing the ferroferric oxide nano-particles is used as a carrier to load the compound bacteria, so that the stability of the microbial bacteria is improved, the microbial bacteria can better resist the impact of the environment, the service life of the microbial bacteria is prolonged, and the slow-release effect of the microbial agent in the remediation of vegetable planting soil is achieved; the nutrient absorption of the vegetables is promoted.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention discloses a bacterial strain and a method for producing pyrroloquinoline quinone. The strain is pseudomonas putida N1-1 which is preserved in the China General Microbiological Culture Collection Center on January 2, 2025, the preservation number is CGMCC NO.33266, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The strain is separated from myxomycete. The bacterial strain is inoculated into a fermentation culture medium, standing fermentation culture is carried out for 7 days at room temperature under the dark condition, a fermentation product is obtained and filtered, fermentation filtrate containing pyrroloquinoline quinine is obtained, and the yield of pyrroloquinoline quinine reaches 12.21 mg / L.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention provides a biological agent with a scale inhibition effect. The biological agent comprises plant lactobacillus, pseudomonas and a saccharomyces cerevisiae strain. The biological scale inhibitor is a mixture of independent fermentation broth containing the three bacteria, the three bacteria are compounded according to different proportions, and the static scale inhibition effect is tested. The preparation is applied to 10L and 100L circulating water simulation systems, the microbial agent is added according to the proportion that the microbial agent is added every 7 days and the total amount of the microbial agent added each time accounts for 0.01%-0.05% of the circulating water, and compared with a control group, an experimental group added with the biological scale inhibitor shows better scale inhibition and algae inhibition effects within four weeks. The microbial agent disclosed by the invention is applied to a 2800m < 3 > circulating water cooling system for a scale inhibition test, and shows a stable scale inhibition effect compared with a conventional chemical scale inhibitor in a test period of 30 days.

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-2 and a complex microbial inoculant thereof in peanut vine silage, and the strain GX-2 is suitable for surviving in the stomach and has an antibacterial effect on staphylococcus aureus, escherichia coli, pseudomonas fluorescens and listeria monocytogenes; the streptococcus non-lactolyticus GX-7 and the streptococcus non-lactolyticus GX-2 are prepared into a complex microbial inoculant, and then the complex microbial inoculant is inoculated into the peanut vines, so that the synergistic fermentation effect can be achieved at the mass ratio of 1: (1-3); after the gestational buffalo is fed with the silage prepared from the complex microbial inoculant, the silage is found to be capable of effectively increasing the contents of total milk solids and non-fat milk solids in the colostrum of the buffalo and relieving the diarrhea of the buffalo, and is safe and good.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses a composite activated carbon filter material and a preparation process thereof, and belongs to the technical field of water treatment materials. The filter material sequentially comprises a modified coconut shell activated carbon matrix, a bimetallic oxide and a biodegradable functional film from inside to outside, the modified coconut shell activated carbon matrix is prepared by a three-step process of acid leaching modification, microwave activation and nitrogen doping; the bimetallic oxide is Fe-Mn oxide nano particles; the biodegradable functional film is composed of a polyvinyl alcohol / polylactic acid blended film and a complex microbial inoculant, and the complex microbial inoculant comprises a mixed flora of pseudomonas, bacillus and nitrifying bacteria. Through the three-layer composite structure of the coconut shell activated carbon matrix, the bimetallic catalyst layer and the biodegradable film, the synergistic effect of physical adsorption, chemical catalysis and biodegradation is achieved. Through step-by-step superposition of the functional layers, the removal efficiency of complex pollutants such as organic matters, heavy metals, ammonia nitrogen and the like is remarkably improved.

The invention belongs to the field of membrane pollution control, and particularly relates to pseudomonas as well as a separation method and application thereof. The invention provides a pseudomonas, which is identified as Pseudomonas knackmussii HITSZ-Q1, is preserved in the Guangdong Microbiological Culture Collection Center, and has the preservation number of GDMCC (China General Microbiological Culture Collection Center) NO: 66162. The strain takes C6-HSL as the unique carbon source and nitrogen source, and the 16S rDNA sequence of the strain is as shown in SEQ ID NO. 1. The strain has a remarkable degradation capability on signal molecules such as C6-HSL, C8-HSL, 3-oxo-C12-HSL and the like, and the highest quenching rate can reach 98.9%. The strain and pseudomonas aeruginosa are co-cultured, so that the signal molecule concentration of the pseudomonas aeruginosa can be effectively reduced, and biofilm formation is inhibited. The strain is further prepared into a carrier in the form of immobilized pellets, the carrier is added into a membrane bioreactor, the operation cycle is prolonged from 6.5 days to 10.5 days, and the membrane pollution delay rate reaches up to 61.5%.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention discloses an environment-friendly bio-organic fertilizer for improving acidified soil and a preparation method of the environment-friendly bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The bio-organic fertilizer is prepared from the following raw materials in parts by weight: 10-20 parts of a composite gel material, 80-100 parts of decomposed livestock and poultry manure, 10-20 parts of a microbial agent, 30-50 parts of modified waste tea biochar, 10-15 parts of urea, 5-10 parts of humic acid, 1-5 parts of calcium superphosphate and 1-5 parts of sodium carboxymethyl cellulose, the microbial agent is prepared by mixing bacillus pseudofirmus, pseudomonas oryzae and azotobacter beijerinckii according to the volume ratio of 1: 1: 1. The bio-organic fertilizer can remarkably improve the pH of soil and increase the content of organic matters in the soil, has good buffering capacity and long-term effect, enhances the water and fertilizer retention capacity of the soil, and has a remarkable effect of increasing both production and income of crops.

The invention relates to Hunan pseudomonas and application thereof. The pseudomonas hunanensis JP1-10 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the pseudomonas hunanensis JP1-10 is CGMCC (China General Microbiological Culture Collection Center) NO: 33318. The strain disclosed by the invention has relatively high salt resistance, has the functions of producing siderophores, IAA, nitrogen fixation and phosphate solubilization, can help plants relieve salt stress and promote growth, and also can antagonize pathogenic bacteria of fusarium oxysporum of cucumbers. Wide application prospects are realized.

The invention relates to the technical field of compound microbial fertilizers, in particular to a biochar-based compound microbial fertilizer for improving saline-alkali soil. The organic fertilizer comprises the following components in parts by weight: 30-50 parts of straws, 10-15 parts of compound microbial capsules, 20-40 parts of compost and 5-10 parts of humic acid. A multi-strain mixture composed of azotobacter chroococcum, pseudomonas and bacillus mucilaginosus is added, and comprehensive improvement of the saline-alkali soil is achieved; the azotobacter chroococcum can convert nitrogen in the air into nitrogen which can be utilized by plants, so that the dependence on chemical fertilizers is reduced; pseudomonas has efficient phosphate solubilizing capacity, and bacillus mucilaginosus can strongly solubilize potassium, so that the soil fertility is comprehensively improved. Composite microorganisms and polylactic acid are subjected to an interfacial polymerization reaction to prepare microcapsules, dopamine hydrochloride is utilized to form a coating on the surfaces of the microcapsules in a self-assembly mode, physical protection can be provided for the microorganisms, the survival rate of the microorganisms in a severe environment is increased, and the acting time of the microorganisms in soil is prolonged.

The invention provides diglyceride oil with a blood sugar reducing effect and a preparation method of the diglyceride oil, and relates to the technical field of functional grease processing. The preparation method comprises the following steps: mixing sea buckthorn seed oil, acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil, and adding glycerol and water to obtain a material A; adding onion pseudomonas lipase and candida antarctica lipase B into the material A, and carrying out primary reaction to obtain a material B; adding candida lipolytica lipase and candida antarctica lipase B into the material B, and carrying out a second reaction to obtain a material C; adding thermomyces lanuginosus lipase into the material C, and carrying out a third reaction to obtain a material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the invention has a good effect in the aspect of reducing blood sugar.