428 results about "Pyruvic acid" patented technology

The invention discloses pyruvate carboxylase (PC) serving as a biomarker for detecting endometriosis and application of the pyruvate carboxylase (PC) to treatment of the endometriosis. The biomarker can be used for detecting the expression level of PC in an endometrial tissue sample through an immunohistochemical method, and when the IHC score is greater than or equal to 4, a subject is judged to be at a high risk of endometriosis or suffered from the endometriosis. Meanwhile, the invention provides an application of drugs (including specific siRNA, ZY-444, octyl gallate and the like) for inhibiting PC expression in treatment of endometriosis, and a corresponding treatment method. The invention provides a new biomarker, target spot and scheme for detection and treatment of endometriosis, and has important clinical application value.

The invention provides a valine production strain and a construction method and application thereof, a designed acetohydroxy acid synthase mutant is that the 88th basic group of an ilvB gene is changed from a to c, the 382nd basic group is changed from a to g, the 413th basic group is changed from c to t, the gene sequence of a designed artificial operon comprises a promoter, an ilvB (A138V) gene or ilvB (Q30K, S128G, A138V) gene of coding mutated acetohydroxy acid synthase, and an ilvN (G20D, I21D, I21D, I21D, I21D, I21D, I21D, I21D) gene. I22F) gene, a pyk gene for coding pyruvate kinase, and a terminator; by designing a specific acetohydroxyacid synthase mutant and related biological materials and artificial operon, the strain constructed by directional modification of the strain by using a pK18mobsacB system gene editing technology based on allele exchange has the advantages of good genetic stability, high fermentation yield and the like, and valine can be stably produced.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

The present disclosure relates to compounds that are capable of inhibiting the mitochondrial pyruvate carrier and promoting hair growth. The disclosure further relates to methods of promoting hair growth or treating conditions or disorders affecting hair growth, such as baldness or alopecia.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

The invention belongs to the technical field of preparation of organic chemicals, and particularly relates to trifluoropyruvic acid and a method for preparing trifluoropyruvic acid from trifluoroacetone. The method comprises the following steps: dissolving trifluoroacetone in a chlorinated solvent, adding a catalyst, introducing chlorine, carrying out a closed reaction, controlling the reaction temperature, cooling to room temperature after the reaction is finished to obtain a 1, 1, 1-trichlorotrifluoroacetone reaction mixture, adding an alkali liquor to carry out a hydrolysis reaction, separating out a water phase after the reaction is finished, and drying to obtain the trifluoropyruvic acid. And continuously applying the chlorination solvent, continuously adding the acid into the water phase to adjust the pH value to 3-4 to obtain a trifluoropyruvic acid water solution, finally extracting and pulping to obtain a wet trifluoropyruvic acid product, and purging with air to obtain a pure trifluoropyruvic acid product. According to the method for preparing trifluoropyruvic acid from trifluoroacetone, the raw materials are easy to obtain, the process is stable, the yield is high, the cost is low, and the invention further provides a trifluoropyruvic acid product.

The present application relates to a mutant gene encoding phosphoenolpyruvate carboxylase containing a mutant 5' untranslated region (5'UTR), a polyhydroxyalkanoate (PHA)-producing microorganism containing the gene, and a PHA production method using the same.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

A system may include a processor-based system including at least one processor and memory comprising instructions. When executed by the processor-based system, the instructions cause the processor-based system to: access metrics of a subject who is being treated with a therapy that includes a magnetic non-parallel electric field (MNPEF) therapy or a magnetic non-parallel magnetic field (MNPMF) therapy, wherein the accessed metrics include at least one analyte, the at least one analyte including one or more of blood glucose data, lactate data, or pyruvate data, free fatty acid data, cholesterol data, and compliance data for complying with the therapy; use the accessed metrics as an input to a machine learning algorithm that is configured to determine a set of therapy parameters for the therapy, and program a controller operably connected to at least one energy field system to deliver the therapy according to the determined set of therapy parameters.

The present disclosure relates to novel compounds that are capable of inhibiting the mitochondrial pyruvate carrier and promoting hair growth. The disclosure further relates to methods of promoting hair growth or treating conditions or disorders affecting hair growth, such as baldness or alopecia.

The invention discloses a synthesis method of tripeptide Fmoc-Pro-Pro-Pro-OH, belongs to the technical field of polypeptide synthesis, and particularly relates to Fmoc-Pro-Pro-Pro-OH prepared by a condensation deprotection reaction of a proline reagent and a proline derivative, the proline reagent comprises Fmoc-Pro-OH or Fmoc-Pro-Pro-OH, and the proline derivative is H-Pro-OtBu. An activating reagent is used in the condensation deprotection reaction, and the mass ratio of the usage amount of the Fmoc-Pro-OH to the usage amount of the activating reagent is 1: (0.1-0.9). Alkali is used in the condensation deprotection reaction, the alkali comprises an alcohol amine compound, the alcohol amine compound is prepared through the reaction of ethanolamine and ethyl 3-bromopyruvate, and the synthesis method of the tripeptide Fmoc-Pro-Pro-Pro-OH is high in yield and purity.

Provided are a theanine-producing strain and use thereof in tea fermentation production. A corynebacterium glutamicum is proposed, which includes an alanine decarboxylase CsAlaDC mutant. The theanine-producing strain is obtained by taking the corynebacterium glutamicum as a starting strain, knocking out in sequence an α-ketoglutarate dehydrogenase E1 subunit gene odhA, a glutamate external transporter gene Ncg11221 and a lactate dehydrogenase gene ldh; and / or expressesing a citrate synthase gene gltA, a pyruvate kinase gene pyk and a glutamate dehydrogenase gene gdh; and / or overexpressing an alanine dehydrogenase alaA and integrating a γ-glutamine synthetase GMAS into a cg1960 pseudogene locus of the corynebacterium glutamicum.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention belongs to the technical field of biological engineering, and discloses a schizochytrium limacinum engineering strain co-expressing a PDC-ACC gene, a construction method and application, and the genetic engineering strain is obtained by taking schizochytrium limacinum as an original strain and expressing a pyruvate decarboxylase compound gene PDC and an acetyl-coenzyme A carboxylase gene ACC in yarrowia lipolytica. The PDC and ACC genes in the schizochytrium limacinum genetic engineering strain disclosed by the invention are cloned to yarrowia lipolytica, namely Po1f. The yield of traditional fermented grease is 43.90 g / L. The engineering strain performs overexpression on PDC and ACC genes, so that the grease content of the schizochytrium limacinum engineering strain is increased by 53.13% and reaches 67.22 g / L, and the DHA content is increased by 36.97% and is increased from 40.71% to 55.76%.

The invention relates to the fields of genetic engineering and microbial fermentation, and discloses a recombinant strain for highly producing terpenoids as well as a construction method and application of the recombinant strain. The recombinant strain is obtained by genetic engineering modification of an original strain, compared with the original strain, the recombinant strain enhances the synthetic route of terpenoids, and the activity of pyruvate kinase, pyruvate dehydrogenase complex, glucose-6-phosphate dehydrogenase and key enzymes for synthesis of terpenoids is enhanced by using a CRISPR activation system; wherein the sgRNA expression promoters of the pyruvate kinase and the pyruvate dehydrogenase complex are respectively oleic acid induction type promoters, and the sgRNA expression promoters of the glucose-6-phosphate dehydrogenase and the terpene compound synthesis key enzyme are respectively copper ion induction type promoters. The recombinant strain can dynamically regulate and control an editing system of the terpenoids, precisely regulate and control the synthetic pathway flux of the terpenoids, and effectively improve the yield of the terpenoids (such as sclareol and bisabolol).

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

The invention discloses a synthesis method of a mono / dibromo pyridine derivative, which comprises the following steps: reacting a pyridine derivative with dimethyl butynedioate and methyl pyruvate at room temperature by using acetonitrile as a solvent to generate a dearomatized heteroaromatic compound; in an oxygen environment, copper bromide is used for brominating the dearomatized heteroaromatic compound to realize dibromination, and if potassium carbonate is additionally added in the reaction, monobromination is realized; and finally, respectively generating mono / dibromopyridine derivatives under hydrochloric acid hydrolysis. A carbon halogen bond of bromopyridine has very high activity, and various reactions such as Suzuki coupling, Sonogashira coupling, Heck reaction, amination reaction and the like can be carried out. On the basis, the polysubstituted bromopyridine can be subjected to selective reaction, and many new ideas can be provided for organic synthesis. The method disclosed by the invention is simple and safe to operate and environment-friendly, the cost is lower by using the copper bromide, and the required reaction conditions are simple.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

The application provides a culture medium and a culture method of ovarian cancer primary cells. The culture medium comprises an MST1 / 2 kinase inhibitor, sodium pyruvate, forskolin, epidermal growth factor, gastrin, fibroblast growth factor 7, nicotinamide, SB431542, and fetal bovine serum. Compared with the existing culture method, the in-vitro culture using the culture medium of the application has higher amplification efficiency; the culture of the ovarian cancer primary cells using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the ovarian cancer primary cell culture.

The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.

The invention discloses a fluorescent probe as well as a preparation method and application thereof. The fluorescent probe has a structural formula as shown in a formula I: # imgabs0 #, wherein R is selected from # imgabs1 #, and X is selected from F, Cl, Br and I. The invention provides the fluorescent probe which is novel in structure and excellent in water solubility, and the fluorescent probe can specifically recognize pyruvate kinase M2 and has the characteristic of more excellent tumor cell imaging.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

Methods for preparing pyruvate kinase (PK) activators or salts or hydrates thereof are provided herein. Synthetic methods for preparing pyruvate kinase (PK) activators, or salts or hydrates thereof, are provided herein. Such activators may be prepared using compounds having formula (A-1) and / or formula (B-1). Specific PK activators and various intermediates prepared by and / or used by the synthetic methods described herein are also disclosed.

The invention discloses application of eupatolide in preparation of a medicine for preventing and treating inflammatory bowel disease, application of eupatolide in preparation of a medicine for preventing and treating inflammatory bowel disease and application of a medicine preparation containing eupatolide in preparation of a medicine for preventing and treating inflammatory bowel disease, and researches that eupatolide (EPT) can be used for preparing the medicine for preventing and treating inflammatory bowel disease through targeting pyruvate kinase isoenzyme 2 (PKM2, Gene ID: 5135). The inflammatory response, glycolysis metabolism and mitochondrial homeostasis are synergistically regulated and controlled, so that the inhibition effect on the inflammatory bowel disease is exerted. Meanwhile, EPT has the advantages of being low in dosage, feasible in oral administration, free of obvious toxic and side effects and the like, and a solid experimental basis is provided for further developing EPT into candidate drugs for preventing and treating inflammatory bowel diseases.

Methods of treating or preventing conditions in a subject are disclosed, the methods including administering to the subject a therapeutically effective amount of ethyl pyruvate, or a derivative or analog thereof, or a pharmaceutical composition thereof. Methods of treating or preventing conditions in a subject including administering to the subject a therapeutically effective amount of ethyl pyruvate, or a derivative or analog thereof, or a pharmaceutical composition thereof, and one or more additional therapeutic agents, are described.

The invention relates to a pyruvate kinase-targeted compound shown as a formula I, a preparation method and application thereof, and application of the pyruvate kinase-targeted compound serving as a plant antibacterial agent, and part of the pyruvate kinase-targeted compound shows excellent bactericidal activity.

The invention discloses a recombinant bacterium for producing L-lactic acid through co-utilization of glucose and xylose as well as a construction method and application of the recombinant bacterium. The invention relates to the technical field of biology, in particular to recombinant bacteria for producing L-lactic acid through co-utilization of glucose and xylose as well as a construction method and application. Methylglyoxal synthase, and / or pyruvate formate lyase, and / or fumarate reductase and / or L-lactic dehydrogenase in receptor Escherichia coli Rec1.0 are modified to obtain recombinant Escherichia coli, the recombinant Escherichia coli is fermented for 24 h, L-lactic acid is the only metabolite, and the yield reaches 2458 g / L; the consumption rate of xylose is 0.76 g / g DCW / h, the consumption rate of glucose is 0.69 g / g DCW / h, and the consumption rate of total sugar reaches 1.45 g / g DCW / h. The recombinant Escherichia coli constructed by the invention can realize efficient utilization of lignocellulose hydrolysate (containing glucose and xylose), and has a wide application prospect.