597 results about "Pyruvic acid" patented technology

The invention relates to the field of gene engineering, and discloses a D-3-phosphoglycerate dehydrogenase mutant, a coding gene thereof and application of the D-3-phosphoglycerate dehydrogenase mutant and the coding gene. Compared with a protein with an amino acid sequence as shown in SEQ ID NO: 1, the mutant comprises mutation at the 131 site and / or the 285 site. The mutant can specifically catalyze a substrate 3-phosphoglycerate to synthesize 3-phosphate-hydroxypyruvic acid, can effectively promote synthesis of L-serine in a metabolic pathway of microorganisms, improves the yield and conversion rate of the L-serine, and reduces the yield of byproducts at the same time.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention provides recombinant escherichia coli for de novo synthesis of anisic acid. At least one of the following improvements is carried out on an escherichia coli starting strain: (1) overexpression of O-methyltransferase PSA-OMT1 from Pleuromyces sapidus, (2) overexpression of O-methyltransferase PSA-OMT1 from Pleuromyces sapidus, and (3) overexpression of O-methyltransferase PSA-OMT1 from Pleuromyces sapidus; the method comprises the following steps: carrying out overexpression on chorismate lyase UbiC derived from E.coli MG1655 or Providencia rustiana, and carrying out the overexpression on the chorismate lyase UbiC derived from E.coli MG1655 or Providencia rustiana; (2) carrying out overexpression on a 3-deoxy-D-arabic-heptanone acid 7-phosphate (DAHP) synthase feedback inhibition mutant AroGD146N derived from E.coli MG1655 on the basis of the step (1); and (3) overexpressing synthase PpsA derived from phosphoenolpyruvate (PEP) on the basis of the step (2), and knocking out a pykF gene for coding pyruvate kinase I. Therefore, in the fermentation process of the recombinant escherichia coli, the accumulation of the anisic acid in the fermentation liquor can be realized, the maximum anisic acid shake flask yield of the recombinant escherichia coli can reach 284.9 mg / L, and a foundation is laid for the subsequent construction of high-yield anisic acid engineering bacteria.

The invention belongs to the technical field of biological medicines, and particularly relates to a pyruvate preparation for treating asthma and a preparation method of the pyruvate preparation. The pyruvate preparation is prepared from the following components in parts by weight: 10 to 12 parts of pyruvate, 10 to 12 parts of sodium chloride, 0.5 to 1 part of citric acid and 500 parts of water for injection. The pH value of the pyruvate preparation is 6 to 7. The pyruvate preparation is used for daily management of asthma patients, the purpose of inhibiting asthma is achieved by controlling increase of the number of inflammatory cells such as eosinophilic granulocytes and reducing inflammatory reaction, the effect of the pyruvate preparation is close to that of similar hormone medicine dexamethasone, and the effect of the pyruvate preparation is improved while the treatment purpose is achieved. The dependence and side effects of patients on hormone drugs are reduced.

The invention belongs to the technical field of medicines, and particularly relates to a pyruvate-containing composite preparation as well as a preparation method and application thereof. The composite preparation containing pyruvate is prepared from the following components in percentage by mass: 0.02 to 0.04 percent of a compound A, 0.05 to 0.1 percent of pyruvate, 0.2 to 2 percent of sodium dihydrogen phosphate, 0.2 to 2 percent of disodium hydrogen phosphate, 0.7 to 0.9 percent of sodium chloride and the balance of water for injection, the structural formula of the compound A is as follows: # imgabs0 #. The compound A and pyruvate in the compound preparation interact with each other, so that lung inflammatory response can be inhibited, the level of inflammatory factors can be reduced, and lung edema can be relieved.

The invention relates to the field of biological medicines, in particular to a composition containing pyruvate as well as a preparation method and application of the composition. The composition containing pyruvate is prepared from the following raw materials in percentage by mass: 0.05 to 0.1 percent of pyruvate, 0.02 to 0.04 percent of bergenin derivative, 5 to 10 percent of water for injection and the balance of propellant, the structural formula of the bergenin derivative is as follows: # imgabs0 #. The composition containing pyruvate can reduce the abundance of pathogenic bacteria and increase the abundance of probiotics, so that the effect of treating lung diseases is achieved by adjusting the respiratory tract micro-ecology.

The present disclosure relates to compounds that are capable of inhibiting the mitochondrial pyruvate carrier and promoting hair growth. The disclosure further relates to methods of promoting hair growth or treating conditions or disorders affecting hair growth, such as baldness or alopecia.

The invention relates to a method for increasing the yield of L-malic acid produced by fermentation by adding ethylene glycol, which comprises the following steps: constructing genetic engineering escherichia coli, and introducing ethylene glycol metabolism genes (overexpression) including glycerol dehydrogenase, lactaldehyde reductase, lactaldehyde dehydrogenase and the like; and overexpression of other genes involving malic acid production, such as malic acid synthase, phosphoenolpyruvate carboxylase and malic acid export protein. The gene engineering strain is an E.coli BW25113 strain of which ldhA, pta, poxB, adhE, pflB, mgsA, frdA, maeA, maeB, yqhD, ycdW and iclR genes are substantially inactivated, and the deleted genes are beneficial to reducing the generation of metabolic byproducts and increasing the accumulation of malic acid. The genetic engineering strain is subjected to fermentation culture by taking glucose and ethylene glycol as carbon sources, and L-malic acid is separated from a culture after culture is finished.

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The invention relates to a repetitive structural unit recombinant protein cpE2 and a preparation method and application thereof. The recombinant protein cpE2 of the repetitive structural unit has self-assembly performance and is formed by modifying an E2 subunit of a pyruvate dehydrogenase compound and dihydrofatty acid amide transacetylase. Specifically, the C end of the E2 protein is arranged at the N end through a connecting peptide by utilizing a circulating recombination principle, so that the recombinant protein cpE2 is constructed. The engineered cpE2 protein cage can be self-assembled into a cage-shaped structure, linking groups can be respectively brought into the protein cage through N-terminal or C-terminal modification of cpE2, the problem that a modifier or a drug is difficult to put into the protein cage by a traditional E2 protein is solved, meanwhile, the specific particle size of the cpE2 protein cage can be controlled through the length of a linking peptide, and the protein cage has a good application prospect. And efficient carrying of goods is achieved.

The invention discloses escherichia coli recombinant bacteria with high glycolic acid yield and a construction method of the escherichia coli recombinant bacteria, and belongs to the technical field of bioengineering. Lactate dehydrogenase ldhA, phosphate transacetylase pta, acetokinase ackA and pyruvate oxidase poxB are knocked out in escherichia coli, key enzymes isocitrate lyase aceA and glyoxylate reductase ghrA in a glycollic acid synthesis path are over-expressed in a genome level, conversion of carbon flow to glycollic acid is enhanced, and the glycolic acid is converted into glycolic acid. On the basis, malic acid synthase AaceB, malic acid synthase GglcB and glycollic acid oxidase glcDEF are knocked out, a branch metabolic pathway is blocked, an efficient strain which is free of plasmids and resistance genes and does not need to induce glycollic acid production is constructed, the accumulation amount of L-valine fermented in a 5L fermentation tank for 48 hours reaches 51.2 g / L, the yield is 0.6 g / g, and the production intensity is 1.06 g / L / h.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of tyrosol, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain, and the recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain. The recombinant yarrowia lipolytica disclosed by the invention expresses an ethanol dehydrogenase encoding gene, and simultaneously expresses any one or a combination of more of a DAHP synthase mutant encoding gene, a chorismate mutase mutant encoding gene, a glucose 6-phosphate dehydrogenase encoding gene, a transketolase encoding gene and a transaldolase encoding gene; meanwhile, a natural promoter of a pyruvate kinase coding gene is truncated. After the recombinant yarrowia lipolytica XJ-TL strain is subjected to fed-batch fermentation, tyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of tyrosol can be achieved.

The invention belongs to the field of metabolic engineering, and discloses a genetically engineered bacterium of high-yield beta-alanine as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli W3110 as an original strain, and is obtained by performing the following gene editing on a genome of the escherichia coli W3110, the genetically engineered bacterium does not express proteins such as lactose operon repressor protein, pyruvate dehydrogenase, acetokinase and the like; protein such as aspartic acid-alpha-decarboxylase, phosphoenolpyruvate carboxylase, pyruvate carboxylase and the like is overexpressed; the phosphoenolpyruvate carboxykinase is replaced by phosphoenolpyruvate carboxykinase from Mannheim succinic acid producing bacteria. The genetically engineered bacterium BA-17 for high yield of beta-alanine, which is constructed by the invention, is fermented in a 5L fermentation tank for 60 hours, the yield of beta-alanine reaches 126.7 g / L, the conversion rate reaches 60.3%, and the production intensity reaches 2.11 g / L / h.

The invention discloses pyruvate carboxylase (PC) serving as a biomarker for detecting endometriosis and application of the pyruvate carboxylase (PC) to treatment of the endometriosis. The biomarker can be used for detecting the expression level of PC in an endometrial tissue sample through an immunohistochemical method, and when the IHC score is greater than or equal to 4, a subject is judged to be at a high risk of endometriosis or suffered from the endometriosis. Meanwhile, the invention provides an application of drugs (including specific siRNA, ZY-444, octyl gallate and the like) for inhibiting PC expression in treatment of endometriosis, and a corresponding treatment method. The invention provides a new biomarker, target spot and scheme for detection and treatment of endometriosis, and has important clinical application value.

The invention discloses a method for catalytic synthesis of malonic acid by using whole cells, and belongs to the field of bioengineering. According to the invention, escherichia coli BL21 (DE3) is taken as a host, an aspartic acid ammonia lyase gene aspA and an aspartic acid-alpha-decarboxylase gene panD, a succinate semialdehyde dehydrogenase gene yneI and a beta-alanine pyruvate transaminase gene, a beta-alanine pyruvate transaminase gene bauA and a malonyl-coenzyme A reductase gene mcr-C are expressed in modules, and recombinant escherichia coli is constructed. The malonic acid is produced by adopting a whole-cell catalysis method, so that the production period is shortened, the production efficiency of the malonic acid is improved, and the yield of the malonic acid can reach 51.0 g / L after the malonic acid is catalyzed for 24 hours.

The invention provides a valine production strain and a construction method and application thereof, a designed acetohydroxy acid synthase mutant is that the 88th basic group of an ilvB gene is changed from a to c, the 382nd basic group is changed from a to g, the 413th basic group is changed from c to t, the gene sequence of a designed artificial operon comprises a promoter, an ilvB (A138V) gene or ilvB (Q30K, S128G, A138V) gene of coding mutated acetohydroxy acid synthase, and an ilvN (G20D, I21D, I21D, I21D, I21D, I21D, I21D, I21D) gene. I22F) gene, a pyk gene for coding pyruvate kinase, and a terminator; by designing a specific acetohydroxyacid synthase mutant and related biological materials and artificial operon, the strain constructed by directional modification of the strain by using a pK18mobsacB system gene editing technology based on allele exchange has the advantages of good genetic stability, high fermentation yield and the like, and valine can be stably produced.

The invention discloses a tobacco leaf surface spraying composition and application thereof, and belongs to the technical field of organic fertilizers. The tobacco leaf surface spraying composition is prepared from the following components in percentage by weight: 0.50 percent to 1.00 percent of lycopene, 0.06 percent to 0.10 percent of phosphoenolpyruvic acid and the balance of water. According to the method, the lycopene and the phosphoenolpyruvic acid with specific contents are sprayed on the leaf surfaces of the tobacco leaves, so that the content of key volatile metabolites closely related to the sensory quality of the flue-cured tobacco in the fresh and sweet flavor type flue-cured tobacco can be increased, the sensory quality of the fresh and sweet flavor type flue-cured tobacco is improved, and the fresh and sweet flavor type of the flue-cured tobacco is enhanced.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

The invention relates to the technical field of microorganisms, and discloses an induction method of VBNC state shigella, a resuscitation medium and application, and the resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, vitamin B12, tryptone, glucose, dipotassium phosphate, monopotassium phosphate, sodium chloride and Tween 80. The resuscitation culture medium obtained by the invention can be used for successfully culturing Shigella in a VBNC state.

The invention belongs to the technical field of biochemical engineering, and particularly relates to a marine metagenome sourced meso-diaminopimelate dehydrogenase mutant and application thereof in D-tryptophan synthesis. According to the invention, a fast evolution method based on Rosetta guidance is adopted to modify meso-diaminopimelate dehydrogenase from a marine metagenome, and a mutant with high catalytic activity on 3-indolepyruvic acid is obtained through screening. The meso-diaminopimelate dehydrogenase mutant disclosed by the invention can be applied to direct catalysis of reductive amination of 3-indolepyruvic acid to generate chiral D-tryptophan, and can also be coupled with L-amino acid deaminase and coenzyme regeneration enzyme to realize configuration inversion from L-tryptophan to D-tryptophan, the substrate concentration reaches 0.1 M, and the product conversion rate is gt; the ee value is gt; therefore, the method has a relatively high practical application value.

The present disclosure relates to compounds that are capable of inhibiting the mitochondrial pyruvate carrier and promoting hair growth. The disclosure further relates to methods of promoting hair growth or treating conditions or disorders affecting hair growth, such as baldness or alopecia.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

The invention belongs to the technical field of preparation of organic chemicals, and particularly relates to trifluoropyruvic acid and a method for preparing trifluoropyruvic acid from trifluoroacetone. The method comprises the following steps: dissolving trifluoroacetone in a chlorinated solvent, adding a catalyst, introducing chlorine, carrying out a closed reaction, controlling the reaction temperature, cooling to room temperature after the reaction is finished to obtain a 1, 1, 1-trichlorotrifluoroacetone reaction mixture, adding an alkali liquor to carry out a hydrolysis reaction, separating out a water phase after the reaction is finished, and drying to obtain the trifluoropyruvic acid. And continuously applying the chlorination solvent, continuously adding the acid into the water phase to adjust the pH value to 3-4 to obtain a trifluoropyruvic acid water solution, finally extracting and pulping to obtain a wet trifluoropyruvic acid product, and purging with air to obtain a pure trifluoropyruvic acid product. According to the method for preparing trifluoropyruvic acid from trifluoroacetone, the raw materials are easy to obtain, the process is stable, the yield is high, the cost is low, and the invention further provides a trifluoropyruvic acid product.

The present invention relates to the field of bioengineering technology and provides a ppc mutant and its application in the fermentative production of L-valine. By performing point mutations on the ppc gene, the expression activity of phosphoenolpyruvate carboxylase is reduced, the competition of the bypass pathway is weakened, the supply of pyruvate precursors is enhanced, and the production capacity and biomass of the valine engineering strain are further improved. It can be seen from the fermentation results that this method can significantly increase the yield of valine, from the original 1.3 g / L to 1.49 g / L, with a 14.6% increase in yield. Therefore, the present invention has application value.

The present application relates to a mutant gene encoding phosphoenolpyruvate carboxylase containing a mutant 5' untranslated region (5'UTR), a polyhydroxyalkanoate (PHA)-producing microorganism containing the gene, and a PHA production method using the same.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention belongs to the technical field of bioengineering, and particularly relates to kluyveromyces marxianus engineering bacteria for synthesizing eicosapentaenoic acid and application of the kluyveromyces marxianus engineering bacteria. According to the engineering bacterium, pyruvate decarboxylase PDC1 genes, alcohol dehydrogenase ADH1 genes and alcohol dehydrogenase ADH2 genes are sequentially knocked out from a kluyveromyces marxianus FIM1 delta ura3 strain; meanwhile, a citrate lyase ACL gene, a pyruvate oxidase PO gene and a phosphate transacetylase PTA gene are inserted into the loci respectively, so that synthesis of acetyl coenzyme A is enhanced; then a plurality of genes are sequentially inserted into a Kluyveromyces marxianus genome, and then a vector of a delta 8 desaturase delta 8D gene is transferred into the Kluyveromyces marxianus genome. The invention also provides a method for efficiently preparing the EPA, namely the Kluyveromyces marxianus engineering strain is used as a fermentation strain, glucose, a yeast extract, peptone and other components are used as a culture medium, and the yield of the EPA produced by culturing and fermenting reaches 8.71 mg / g of dry cell weight.

The invention belongs to the technical field of biological medicines, and particularly relates to a pyruvate preparation for treating non-small cell lung cancer and a preparation method of the pyruvate preparation. The pyruvate preparation is prepared from the following components in parts by weight: 0.03 to 0.05 part of sodium pyruvate, 0.01 to 0.03 part of synergist and 100 parts of water for injection. The preparation method of the synergist comprises the following steps: adding sodium pyruvate into a 3, 4-diaminophenol solution for reaction to obtain an intermediate 1; reacting the intermediate 1 with 4-chloro-1, 2-phenylenediamine to obtain an intermediate 2; reacting p-hydroxybenzaldehyde with propynyl chloride to obtain an intermediate 3; reacting the intermediate 3 with 1-azido-4-chlorobenzene to obtain an intermediate 4; and reacting the intermediate 2 with the intermediate 4 to obtain the synergist. Experimental results show that the synergistic agent and the sodium pyruvate are matched to achieve an excellent anti-tumor effect, and particularly, the selectivity of inhibition on non-small cell lung cancer tumors is shown.

A system may include a processor-based system including at least one processor and memory comprising instructions. When executed by the processor-based system, the instructions cause the processor-based system to: access metrics of a subject who is being treated with a therapy that includes a magnetic non-parallel electric field (MNPEF) therapy or a magnetic non-parallel magnetic field (MNPMF) therapy, wherein the accessed metrics include at least one analyte, the at least one analyte including one or more of blood glucose data, lactate data, or pyruvate data, free fatty acid data, cholesterol data, and compliance data for complying with the therapy; use the accessed metrics as an input to a machine learning algorithm that is configured to determine a set of therapy parameters for the therapy, and program a controller operably connected to at least one energy field system to deliver the therapy according to the determined set of therapy parameters.

The invention belongs to the technical field of sewage treatment, and particularly relates to a composite carbon source additive and a preparation method thereof. The composite carbon source additive comprises the following components in parts by weight: 30-40 parts of bagasse, 8-12 parts of a polyvinyl alcohol derivative, 2-4 parts of a functional filler, 5-8 parts of potassium citrate and 5-8 parts of sodium pyruvate, the preparation process of the polyvinyl alcohol derivative comprises the following steps: adding polyvinyl alcohol, 1, 3, 5-benzene trisulfonic acid, triethylamine and 4-dimethylaminopyridine into dichloromethane to react, filtering, washing and drying; the polyvinyl alcohol derivative is obtained. The composite carbon source additive can be slowly released in the denitrification process and continuously and stably provide a carbon source, the denitrification process is promoted, and the denitrification efficiency is improved.