17 results about "Ethanol yield" patented technology

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

The invention discloses saccharomyces cerevisiae XJ-YSSCE010-1 with high temperature resistance, high ethanol yield and low fusel oil yield and application of the saccharomyces cerevisiae XJ-YSSCE010-1, and belongs to the technical field of microbial fermentation. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention is separated and screened from Maotai-flavor liquor yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31174. The fusel oil comprises the following main components: n-propanol, isobutanol, isoamyl alcohol, phenethyl alcohol and the like, wherein isobutanol and isoamyl alcohol have the highest toxicity. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention shows the advantages of high yield of ethanol, low yield of fusel oil and no production of isobutanol and isoamyl alcohol in liquid fermentation and simulated high-temperature stacking fermentation experiments, and the saccharomyces cerevisiae XJ-YSSCE010-1 is applied to preparation of white spirit, so that the production efficiency of the white spirit is improved, and the quality of the white spirit is improved.

The invention relates to a gene mutation dominant anaerobic strain and an acquisition method and application thereof.The method comprises the steps that a production line strain is prepared into first freeze-dried bacterial powder, then pre-purification inoculated culture and separation treatment are conducted in sequence, and a plurality of anaerobic single colonies are obtained and then subjected to expanding culture; screening a dominant anaerobic strain with gene mutation, which can increase the ethanol yield by more than 5%; after the dominant anaerobic strain with gene mutation obtained by the method is applied to industrial tail gas fermentation again, the yield of ethanol can be increased; compared with an anaerobic strain cultivated in a laboratory, the dominant anaerobic strain with the gene mutation is easier to cultivate; according to the application, a dominant anaerobic strain with gene mutation is applied to fermentation of industrial tail gas again, so that the yield of converting the industrial tail gas into renewable fuels such as ethanol and butanol can be increased to 5% or above.

The invention relates to the technical field of fermentation engineering and wine brewing, and discloses a low-alcohol fruit wine brewing process based on compound microorganism synergistic fermentation, which comprises the following steps: inoculating Meijie yeast into clarified fruit juice, adding L-leucine, culturing under controlled temperature, and removing free iron ions by biological chelation until the liquid level becomes red; then inoculating saccharomyces cerevisiae, starting a trace oxygen introduction and ORP (oxidation-reduction potential) system after main fermentation is started, and controlling potential to carry out metabolism redirected fermentation in a micro-aerobic interval until residual sugar reaches the standard; after the fermentation is finished, adding an alkaline regulator to increase the pH value, inducing the haematochrome-iron compound to flocculate and settle, separating to remove the precipitate, and adding an acidic regulator to adjust the pH value to obtain the low-alcohol fruit wine. According to the method disclosed by the invention, a brewer's yeast respiratory chain is blocked and oxidative stress is applied by utilizing a metabolic redirection strategy under precursor-induced biological iron removal and micro-aerobic environment, so that a carbon flux is promoted to flow to a glycerol synthesis path, the ethanol yield is reduced, and the glycerol content is greatly increased.

The invention discloses a continuous gas fermentation regulation and control method, and solves the technical problem of low ethanol yield of a fermentation system in the prior art under the condition of fluctuation of industrial tail gas. The regulation and control method comprises the following steps: acquiring the current acetic acid concentration, ethanol concentration, carbon monoxide demand and carbon monoxide supply of the fermentation system; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within a set range and the carbon monoxide supply amount is greater than the carbon monoxide demand amount, increasing the process parameters of the fermentation system in multiple stages until the dilution rate reaches a first set value; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within the set range and the carbon monoxide supply amount is smaller than the carbon monoxide demand amount, the dilution rate of the fermentation system is lowered in multiple stages until the dilution rate reaches a second set value, and the first set value is larger than the second set value. The continuous gas fermentation regulation and control method provided by the invention improves the yield of ethanol.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The application discloses application of soluble lignin in the fields of serving as an enhancer of ethanol fermentation and / or improving stress resistance of yeast under fermentation environmental stress conditions, and adds the soluble lignin to an ethanol fermentation system, so that problems such as pH instability, oxidation damage and heavy metal inhibition in the ethanol fermentation can be solved synchronously, yeast survival rate and ethanol yield are significantly improved, process steps are simplified, and cost is reduced, and the application is suitable for industrialized production of ethanol from raw materials such as agricultural and forestry waste, and provides a green and practical technical path for efficient utilization of whole components of lignocellulose. In addition, the soluble lignin can be recycled, and still has relatively high enhancement effect after recycling for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

This invention belongs to the field of aviation fuel technology and provides a method for producing sustainable aviation fuel using straw. First, straw is converted into syngas; then, the syngas and dimethyl ether undergo a catalytic reaction using a primary molecular sieve catalyst. The resulting catalytic product then undergoes a secondary metal catalyst reaction, followed by separation to obtain ethanol; the ethanol is then converted into ethylene; finally, the ethylene is used to produce aviation fuel. The preparation method of the primary molecular sieve catalyst is as follows: a phosphoric acid solution is mixed with deionized water, and a template agent is added (the molar ratio of phosphoric acid to template agent is 1:(0.5-0.55). An aluminum source, a cerium source, and silica sol are added under stirring to obtain a gel; the gel is heated and crystallized; the product is centrifuged, washed until neutral, dried, and calcined; the calcined product is immersed in a zinc nitrate solution, dried, and calcined to obtain a zinc-loaded product; the zinc-loaded product is treated with citric acid solution for dealuminization, followed by calcination to obtain the final product. This invention can significantly improve the ethanol yield in the aviation fuel production process.

The invention belongs to the technical field of biomass conversion and utilization, and particularly provides a method for efficiently producing ethanol by utilizing lignocellulose. Additives such as tea saponin are introduced in the synchronous saccharification and fermentation stage, the conversion efficiency of the lignocellulose raw material can be remarkably improved, and the ethanol yield is remarkably improved. The method specifically comprises the following steps: firstly, pretreating a lignocellulose raw material by adopting a deep eutectic solvent, combining with a synchronous saccharification and fermentation technology, and introducing a specific additive in a fermentation stage, so that the conversion efficiency of the lignocellulose raw material is remarkably improved, and the ethanol yield is improved. The method for efficiently producing ethanol is high in ethanol yield, wide in raw material source and suitable for lignocellulose raw materials such as pennisetum hydridum with a compact fiber structure, has sustainable development, and provides powerful support for industrial application of lignocellulose energy.

The present application relates to a kind of cellulose ethanol production closed loop recycling system, comprising the following steps: S1, cellulose biomass is placed in sodium citrate buffer solution, sulfomethylization modified lignin is added, and then cellulase is added, and cellulase enzymatic saccharification is carried out, and after saccharification, the sugar solution is inoculated with saccharomyces cerevisiae and enters fermentation process, and monosaccharide is converted into ethanol;S2, sulfomethylization modified lignin is recovered from the product obtained in S1 by solid-liquid separation method, and is regenerated by alkali elution or resulfonation, and is used in S1 for the next round of cellulose ethanol production process.Compared with prior art, the present application can reduce the non-productive adsorption of cellulase and lignin, while reducing the toxicity of inhibitors, thereby improving the saccharification and detoxification efficiency, and ultimately improving the ethanol yield;The present application recycles and utilizes the byproduct lignin in the ethanol production process, and constructs an efficient detoxification saccharification and resource recycling closed loop system.

The application discloses a high-ethanol-yield Saccharomyces cerevisiae engineering strain based on molasses addition as a substrate and a construction method and application thereof, the engineering strain takes Saccharomyces cerevisiae as an original strain, overexpresses a PIR3 gene, or simultaneously overexpresses the PIR3 gene and a SPI1 gene. The application mines the target sites PIR3 and SPI1 which can relieve molasses stress, overexpression of the target sites can increase cell viability, improves the biosynthesis efficiency of Saccharomyces cerevisiae under the condition of molasses as a substrate, and can effectively improve the ethanol yield.

The invention discloses a preparation method of functionalized lignin, which comprises the following steps: carrying out in-situ modification on lignocellulose by using p-toluenesulfonic acid and ethylene glycol to obtain the functionalized lignin with surface activity; meanwhile, the functionalized lignin is used as a surfactant, so that the ethanol concentration and the ethanol yield can be effectively improved in the saccharification and fermentation process, and the ethanol yield can reach 90% or above. The functionalized lignin can be synchronously prepared when the lignocellulose raw material is pretreated, so that the whole process from the raw material to the product is simplified; and the functionalized lignin is derived from a byproduct in a lignocellulose raw material pretreatment process and is cheaper than commercial surface activity, so that the comprehensive cost of producing ethanol by saccharification and fermentation can be effectively reduced.

The invention discloses a yeast composite leavening agent with low ethanol yield and high aroma yield and application thereof, and belongs to the technical field of microorganisms. The method solves the problems that the fermented beverage is unbalanced in flavor, insufficient in fragrance and too high in acidity due to the fact that the saccharomyces cerevisiae is independently used for fermentation. After the non-saccharomyces cerevisiae is added, the hansenula polymorpha strain HHT134 and the saccharomyces cerevisiae JM9 in the grape juice promote growth mutually, meanwhile, aroma substances richer than those of single-bacterium fermentation can be generated, generation of bad flavor can be reduced, and the fermented beverage is endowed with more coordinated taste and flavor. The invention provides a new method for deep processing of yellow peaches and development of low-alcohol beverages.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

The invention provides a method and device for preparing ethyl alcohol through photoelectrocatalytic reduction of carbon dioxide. The method comprises the following steps that S1, an MOF parent material with high adsorption and catalytic performance is screened, specifically, the MOF parent material meeting the requirement is screened out by conducting CO2 adsorption capacity testing and catalytic activity preliminary evaluation on multiple MOF materials; the invention relates to a method and a device for preparing ethanol by photoelectrocatalytic reduction of carbon dioxide. According to the method and the device for preparing ethanol by photoelectrocatalytic reduction of carbon dioxide provided by the invention, Cu single atoms are loaded on the modified MOF material, so that the catalyst has high atom utilization rate and good catalytic activity and selectivity; the solar-driven photoelectric catalytic reaction device is built and optimized, so that the reaction can be carried out under the optimal condition to improve the ethanol generation efficiency, and the reaction process is monitored and analyzed by multiple means to provide theoretical support assistance for optimizing a photoelectric catalytic reaction system so as to further improve the ethanol yield and the energy utilization efficiency.

The application discloses a recombinant engineering bacterium with ThlA gene knockout and industrial gas source domestication and application thereof. The recombinant engineering bacterium is Clostridium autoethanogenum engineering bacterium with the preservation number of GDMCC No: 67962. The application optimizes carbon flow distribution of the C. autoethanogenum from the metabolic pathway level by using the CRISPR / Cas9 gene editing technology to knock out the acetyl coenzyme A acetyltransferase coding gene ThlA (gene number: CLAU_0409), and reduces by-product generation. On this basis, the gene edited strain is further subjected to laboratory directional domestication to strengthen the tolerance and adaptability of the strain to the complex environment of steel industry tail gas, and finally the growth rate, ethanol selectivity and ethanol yield of the strain are simultaneously improved, thereby providing core strain support for industrialization landing of resource utilization of the steel industry tail gas.