27 results about "Ethanol yield" patented technology

The invention discloses an application of soluble lignin as a synergist for ethanol fermentation and / or improvement of stress resistance of yeast under a fermentation environment stress condition, the soluble lignin is added into an ethanol fermentation system, and the problems of pH instability, oxidative damage, heavy metal inhibition and the like in ethanol fermentation can be synchronously solved; the survival rate of yeast and the yield of ethanol are remarkably improved, the process steps are simplified, the cost is reduced, the method is suitable for ethanol industrial production of agricultural and forestry wastes and other raw materials, and a green and practical technical path is provided for efficient utilization of all components of lignocellulose. In addition, the soluble lignin disclosed by the invention can also be recycled, can still keep a relatively high synergistic effect after being recycled for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention discloses saccharomyces cerevisiae XJ-YSSCE010-1 with high temperature resistance, high ethanol yield and low fusel oil yield and application of the saccharomyces cerevisiae XJ-YSSCE010-1, and belongs to the technical field of microbial fermentation. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention is separated and screened from Maotai-flavor liquor yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31174. The fusel oil comprises the following main components: n-propanol, isobutanol, isoamyl alcohol, phenethyl alcohol and the like, wherein isobutanol and isoamyl alcohol have the highest toxicity. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention shows the advantages of high yield of ethanol, low yield of fusel oil and no production of isobutanol and isoamyl alcohol in liquid fermentation and simulated high-temperature stacking fermentation experiments, and the saccharomyces cerevisiae XJ-YSSCE010-1 is applied to preparation of white spirit, so that the production efficiency of the white spirit is improved, and the quality of the white spirit is improved.

The invention relates to a gene mutation dominant anaerobic strain and an acquisition method and application thereof.The method comprises the steps that a production line strain is prepared into first freeze-dried bacterial powder, then pre-purification inoculated culture and separation treatment are conducted in sequence, and a plurality of anaerobic single colonies are obtained and then subjected to expanding culture; screening a dominant anaerobic strain with gene mutation, which can increase the ethanol yield by more than 5%; after the dominant anaerobic strain with gene mutation obtained by the method is applied to industrial tail gas fermentation again, the yield of ethanol can be increased; compared with an anaerobic strain cultivated in a laboratory, the dominant anaerobic strain with the gene mutation is easier to cultivate; according to the application, a dominant anaerobic strain with gene mutation is applied to fermentation of industrial tail gas again, so that the yield of converting the industrial tail gas into renewable fuels such as ethanol and butanol can be increased to 5% or above.

The invention relates to the technical field of fermentation engineering and wine brewing, and discloses a low-alcohol fruit wine brewing process based on compound microorganism synergistic fermentation, which comprises the following steps: inoculating Meijie yeast into clarified fruit juice, adding L-leucine, culturing under controlled temperature, and removing free iron ions by biological chelation until the liquid level becomes red; then inoculating saccharomyces cerevisiae, starting a trace oxygen introduction and ORP (oxidation-reduction potential) system after main fermentation is started, and controlling potential to carry out metabolism redirected fermentation in a micro-aerobic interval until residual sugar reaches the standard; after the fermentation is finished, adding an alkaline regulator to increase the pH value, inducing the haematochrome-iron compound to flocculate and settle, separating to remove the precipitate, and adding an acidic regulator to adjust the pH value to obtain the low-alcohol fruit wine. According to the method disclosed by the invention, a brewer's yeast respiratory chain is blocked and oxidative stress is applied by utilizing a metabolic redirection strategy under precursor-induced biological iron removal and micro-aerobic environment, so that a carbon flux is promoted to flow to a glycerol synthesis path, the ethanol yield is reduced, and the glycerol content is greatly increased.

The invention belongs to the technical field of microorganisms, and relates to schizosaccharomyces japonicus capable of efficiently metabolizing malic acid and an application of the schizosaccharomyces japonicus. The schizosaccharomyces japonicus strain FBKL2.9SZJ-37 capable of efficiently metabolizing malic acid is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20251245, the preservation time is June 4, 2025, the strain shows the efficient malic acid metabolizing ability during fermentation, 2.959 g / L of malic acid is consumed after the strain is cultured in an MDL screening culture medium for 7 days, and the highest degradation rate in all strains is 98.64%; 2.965 g / L of malic acid is consumed after fermentation of a simulated malic acid fermentation medium (SMF) for 16 days, and the degradation rate of malic acid is 98.83%; the malic acid consumption after fermentation of the cabernet sauvignon, the rosa sterilis and the rosa roxburghii tratt is 2.93 g / L, 28.73 g / L and 32.40 g / L respectively, and the malic acid degradation rates are 98.32%, 60.50% and 64.98% respectively. The strain is excellent in fermentation performance, stable in ethanol yield, high in tolerance, relatively high in glycerol yield and relatively low in volatile acid level.

The invention discloses a continuous gas fermentation regulation and control method, and solves the technical problem of low ethanol yield of a fermentation system in the prior art under the condition of fluctuation of industrial tail gas. The regulation and control method comprises the following steps: acquiring the current acetic acid concentration, ethanol concentration, carbon monoxide demand and carbon monoxide supply of the fermentation system; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within a set range and the carbon monoxide supply amount is greater than the carbon monoxide demand amount, increasing the process parameters of the fermentation system in multiple stages until the dilution rate reaches a first set value; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within the set range and the carbon monoxide supply amount is smaller than the carbon monoxide demand amount, the dilution rate of the fermentation system is lowered in multiple stages until the dilution rate reaches a second set value, and the first set value is larger than the second set value. The continuous gas fermentation regulation and control method provided by the invention improves the yield of ethanol.

The application discloses a fermentation method for improving the tolerance of Clostridium aceticum to high-concentration CO gas and belongs to the technical field of microbial fermentation. In view of the substrate inhibition of high-concentration CO on Clostridium aceticum, the application proposes an asymmetric gas velocity regulation strategy of "over-regulation and slow increase". When the CO volume concentration is increased from C1 to C2, the inlet gas velocity is excessively decreased to V2=k*(C1 / C2)*V1, k=0.3-0.7; the low gas velocity is maintained for T1>=24 h to make the bacteria adapt to the high CO environment; and then the gas velocity is gradually increased to V3>=(C1 / C2)*V1 in stages. The method can make the Clostridium aceticum restore growth and metabolism within 2-3 days after the CO concentration is switched to 90%, the ethanol yield is increased by more than 18% compared with the 60% CO culture, and the method does not need genetic modification and can be directly adapted to the industrial tail gas with a CO content of 80-95%, thereby significantly improving the efficiency of high-concentration CO fermentation and the resource utilization value of the industrial tail gas.

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof. In order to enrich the source of strains with low-yield higher alcohols, the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is separated and screened from fermented grains of Luzhou-flavor liquor through primary screening and secondary screening, then normal-pressure plasma mutation breeding is performed on the Wickerhamomyces anomalus Wa9, and the Wickerhamomyces anomalus Wa9 which is relatively low in higher alcohol yield and good in environment tolerance is obtained through primary screening and secondary screening. The Wickerhamomyces anomalus WA27 has the advantages that the Wickerhamomyces anomalus WA27 is high in yield, environmental tolerance and ethanol conversion rate, and the preservation number of the Wickerhamomyces anomalus WA27 is CCTCC NO: M 20252211. The strain not only maintains good ethanol yield, but also significantly reduces the generation of higher alcohols. And the strain is obtained through ARTP mutagenesis, is good in hereditary stability and is suitable for industrial production. When the prepared distiller's yeast is used for fermenting white spirit, a white spirit product with better flavor and taste can be obtained.

The invention provides a method for extracting alcohol by using a by-product after garlic oil production, which comprises a pretreatment step, a fermentation step, a distillation step and a refining step, and through the combination of crushing and high-temperature cooking, starch in the garlic by-product is fully gelatinized, the cell wall structure is destroyed, and the alcohol content is reduced; more importantly, infectious microbes possibly carried in the raw materials are effectively killed, and a solid foundation is laid for subsequent pure fermentation; amylase and saccharifying enzyme are selectively added for enzymolysis, so that macromolecular carbohydrates are further accurately degraded into fermentable sugars which can be directly utilized by yeast, the saccharification rate of the raw materials is remarkably improved, and a core guarantee is directly provided for improving the final ethanol yield. Through combination of normal pressure rectification and pressurized rectification, ethanol is separated and concentrated, and through deep processing units such as molecular sieve dehydration or blending and aging, diversified alcohol products from industrial fuel and medical disinfection to high-end drinking can be flexibly produced by the same basic process, so that the application range and the economic value are expanded.

The invention relates to the technical field of genetically engineered microorganisms, and particularly discloses a saccharomyces cerevisiae genetically engineered bacterium with ATF1 and ALD6 genes knocked out as well as a construction method and application of the saccharomyces cerevisiae genetically engineered bacterium, and the preservation number of the saccharomyces cerevisiae genetically engineered bacterium is CCGMCC No.35992. The original saccharomyces cerevisiae gene knockout ATF1 and ALD6 genes are modified to obtain the saccharomyces cerevisiae genetically engineered bacterium, and compared with the original saccharomyces cerevisiae, the saccharomyces cerevisiae genetically engineered bacterium with the ATF1 and ALD6 genes knockout shows higher ethanol yield and lower acetate substance concentration in the beer fermentation process; the ethanol yield and the taste of the upper fermentation type beer fermented by the saccharomyces cerevisiae are greatly improved.

The invention relates to the technical field of microorganisms, and particularly discloses saccharomyces cerevisiae IMPHA-6 and application thereof in fermentation production of ethanol. The invention provides a Saccharomyces cerevisiae strain capable of resisting multiple stress, which is Saccharomyces cerevisiae IMPHA-6, and the preservation number of the Saccharomyces cerevisiae strain is CCTCC (China Center for Type Culture Collection) NO: M 20251412. The saccharomyces cerevisiae strain can solve the problem of low fermentation efficiency caused by a composite inhibitor (such as potassium chloride, ethanol, furfural and vanillin) in the lignocellulose hydrolysate, and can remarkably improve the reducing sugar utilization rate and ethanol yield during fermentation of the corn straw cellulose hydrolysate; a novel efficient microbial resource and method are provided for biomass energy production, and promotion of industrial production of cellulosic ethanol is facilitated.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The application discloses application of soluble lignin in the fields of serving as an enhancer of ethanol fermentation and / or improving stress resistance of yeast under fermentation environmental stress conditions, and adds the soluble lignin to an ethanol fermentation system, so that problems such as pH instability, oxidation damage and heavy metal inhibition in the ethanol fermentation can be solved synchronously, yeast survival rate and ethanol yield are significantly improved, process steps are simplified, and cost is reduced, and the application is suitable for industrialized production of ethanol from raw materials such as agricultural and forestry waste, and provides a green and practical technical path for efficient utilization of whole components of lignocellulose. In addition, the soluble lignin can be recycled, and still has relatively high enhancement effect after recycling for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

This invention belongs to the field of aviation fuel technology and provides a method for producing sustainable aviation fuel using straw. First, straw is converted into syngas; then, the syngas and dimethyl ether undergo a catalytic reaction using a primary molecular sieve catalyst. The resulting catalytic product then undergoes a secondary metal catalyst reaction, followed by separation to obtain ethanol; the ethanol is then converted into ethylene; finally, the ethylene is used to produce aviation fuel. The preparation method of the primary molecular sieve catalyst is as follows: a phosphoric acid solution is mixed with deionized water, and a template agent is added (the molar ratio of phosphoric acid to template agent is 1:(0.5-0.55). An aluminum source, a cerium source, and silica sol are added under stirring to obtain a gel; the gel is heated and crystallized; the product is centrifuged, washed until neutral, dried, and calcined; the calcined product is immersed in a zinc nitrate solution, dried, and calcined to obtain a zinc-loaded product; the zinc-loaded product is treated with citric acid solution for dealuminization, followed by calcination to obtain the final product. This invention can significantly improve the ethanol yield in the aviation fuel production process.

The invention belongs to the technical field of biomass conversion and utilization, and particularly provides a method for efficiently producing ethanol by utilizing lignocellulose. Additives such as tea saponin are introduced in the synchronous saccharification and fermentation stage, the conversion efficiency of the lignocellulose raw material can be remarkably improved, and the ethanol yield is remarkably improved. The method specifically comprises the following steps: firstly, pretreating a lignocellulose raw material by adopting a deep eutectic solvent, combining with a synchronous saccharification and fermentation technology, and introducing a specific additive in a fermentation stage, so that the conversion efficiency of the lignocellulose raw material is remarkably improved, and the ethanol yield is improved. The method for efficiently producing ethanol is high in ethanol yield, wide in raw material source and suitable for lignocellulose raw materials such as pennisetum hydridum with a compact fiber structure, has sustainable development, and provides powerful support for industrial application of lignocellulose energy.

The present application relates to a kind of cellulose ethanol production closed loop recycling system, comprising the following steps: S1, cellulose biomass is placed in sodium citrate buffer solution, sulfomethylization modified lignin is added, and then cellulase is added, and cellulase enzymatic saccharification is carried out, and after saccharification, the sugar solution is inoculated with saccharomyces cerevisiae and enters fermentation process, and monosaccharide is converted into ethanol;S2, sulfomethylization modified lignin is recovered from the product obtained in S1 by solid-liquid separation method, and is regenerated by alkali elution or resulfonation, and is used in S1 for the next round of cellulose ethanol production process.Compared with prior art, the present application can reduce the non-productive adsorption of cellulase and lignin, while reducing the toxicity of inhibitors, thereby improving the saccharification and detoxification efficiency, and ultimately improving the ethanol yield;The present application recycles and utilizes the byproduct lignin in the ethanol production process, and constructs an efficient detoxification saccharification and resource recycling closed loop system.

The application discloses a high-ethanol-yield Saccharomyces cerevisiae engineering strain based on molasses addition as a substrate and a construction method and application thereof, the engineering strain takes Saccharomyces cerevisiae as an original strain, overexpresses a PIR3 gene, or simultaneously overexpresses the PIR3 gene and a SPI1 gene. The application mines the target sites PIR3 and SPI1 which can relieve molasses stress, overexpression of the target sites can increase cell viability, improves the biosynthesis efficiency of Saccharomyces cerevisiae under the condition of molasses as a substrate, and can effectively improve the ethanol yield.

The invention discloses a preparation method of functionalized lignin, which comprises the following steps: carrying out in-situ modification on lignocellulose by using p-toluenesulfonic acid and ethylene glycol to obtain the functionalized lignin with surface activity; meanwhile, the functionalized lignin is used as a surfactant, so that the ethanol concentration and the ethanol yield can be effectively improved in the saccharification and fermentation process, and the ethanol yield can reach 90% or above. The functionalized lignin can be synchronously prepared when the lignocellulose raw material is pretreated, so that the whole process from the raw material to the product is simplified; and the functionalized lignin is derived from a byproduct in a lignocellulose raw material pretreatment process and is cheaper than commercial surface activity, so that the comprehensive cost of producing ethanol by saccharification and fermentation can be effectively reduced.

The invention discloses a yeast composite leavening agent with low ethanol yield and high aroma yield and application thereof, and belongs to the technical field of microorganisms. The method solves the problems that the fermented beverage is unbalanced in flavor, insufficient in fragrance and too high in acidity due to the fact that the saccharomyces cerevisiae is independently used for fermentation. After the non-saccharomyces cerevisiae is added, the hansenula polymorpha strain HHT134 and the saccharomyces cerevisiae JM9 in the grape juice promote growth mutually, meanwhile, aroma substances richer than those of single-bacterium fermentation can be generated, generation of bad flavor can be reduced, and the fermented beverage is endowed with more coordinated taste and flavor. The invention provides a new method for deep processing of yellow peaches and development of low-alcohol beverages.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

The invention provides a method and device for preparing ethyl alcohol through photoelectrocatalytic reduction of carbon dioxide. The method comprises the following steps that S1, an MOF parent material with high adsorption and catalytic performance is screened, specifically, the MOF parent material meeting the requirement is screened out by conducting CO2 adsorption capacity testing and catalytic activity preliminary evaluation on multiple MOF materials; the invention relates to a method and a device for preparing ethanol by photoelectrocatalytic reduction of carbon dioxide. According to the method and the device for preparing ethanol by photoelectrocatalytic reduction of carbon dioxide provided by the invention, Cu single atoms are loaded on the modified MOF material, so that the catalyst has high atom utilization rate and good catalytic activity and selectivity; the solar-driven photoelectric catalytic reaction device is built and optimized, so that the reaction can be carried out under the optimal condition to improve the ethanol generation efficiency, and the reaction process is monitored and analyzed by multiple means to provide theoretical support assistance for optimizing a photoelectric catalytic reaction system so as to further improve the ethanol yield and the energy utilization efficiency.

The invention discloses thermoanaerobacter thermosaccharolyticus for producing ethanol and application of the thermoanaerobacter thermosaccharolyticus, and belongs to the technical field of genetic engineering. According to the invention, metabolic pathways of lactic acid, acetic acid, propylene glycol and the like of wild thermoanaerobacter thermolyticus are blocked through metabolic engineering transformation, so that the engineering bacteria of thermoanaerobacter thermolyticus of which the metabolite is mainly ethanol are obtained. The thermoanaerobacter thermolyticus engineering bacteria can produce ethanol through fermentation by taking glucose, xylose or arabinose as a carbon source, when glucose is taken as the carbon source, 25g / L glucose can be consumed after fermentation is carried out for 24h, and the ethanol yield is 94.5%; when xylose is used as a carbon source, 25 g / L of xylose can be consumed after fermentation is performed for 24 h, and the yield of ethanol is 91.4%; when glucose, xylose and arabinose are used as carbon sources, the ethanol yield is 90.5% after 24 hours of fermentation; in the fermentation, byproducts such as lactic acid, acetic acid and propylene glycol are not accumulated.

The application discloses a recombinant engineering bacterium with ThlA gene knockout and industrial gas source domestication and application thereof. The recombinant engineering bacterium is Clostridium autoethanogenum engineering bacterium with the preservation number of GDMCC No: 67962. The application optimizes carbon flow distribution of the C. autoethanogenum from the metabolic pathway level by using the CRISPR / Cas9 gene editing technology to knock out the acetyl coenzyme A acetyltransferase coding gene ThlA (gene number: CLAU_0409), and reduces by-product generation. On this basis, the gene edited strain is further subjected to laboratory directional domestication to strengthen the tolerance and adaptability of the strain to the complex environment of steel industry tail gas, and finally the growth rate, ethanol selectivity and ethanol yield of the strain are simultaneously improved, thereby providing core strain support for industrialization landing of resource utilization of the steel industry tail gas.

The invention discloses a single cell surface stereocomplex polylactic acid silicon dioxide radiation refrigeration shell layer material and a preparation method thereof, L-polylactic acid, D-polylactic acid and silicon dioxide are adopted as raw materials, a radiation refrigeration shell layer is constructed on the surface of a yeast single cell, and yeast cells are endowed with good environmental tolerance; the cell activity of yeast cells in a high-temperature environment is improved, so that the ethanol yield is improved. The stereocomplex polylactic acid silicon dioxide radiation refrigeration shell material can reduce the surface temperature of an object with zero energy consumption by reflecting visible light and near-infrared light and radiating mid-infrared light, improves the tolerance of cells to light and heat, provides protection for the cells, and further provides light, heat and high temperature resistant immobilized cells for simultaneous saccharification and fermentation. The radiation refrigeration material can reflect visible light and near infrared rays and emit intermediate infrared rays, so that cooling and refrigeration are achieved. A radiation refrigeration technology and a cell immobilization technology are combined, and cells are immobilized by a radiation refrigeration material, so that the tolerance of yeast cells to light and heat is improved.