281 results about "Ethanol yield" patented technology

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.

The invention discloses a Saccharomyces cerevisiae strain and a method for producing ethanol by fermenting stalks, the strain is Saccharomyces cerevisiae DQ1, the collection No. is CGMCC No.2528, the strain can tolerate methanoic acid, acetic acid and furfural type inhibitors in hydrolyzate of the stalks, tolerate high glucose concentration and ethanol concentration and have wide growth temperature, and the strain is fermented at high temperature; the method for producing the ethanol by fermenting the stalks comprises two-stage culture of seeds, wherein the first stage culture is carried out in a synthetic culture medium for 16-24 hours, the second stage culture is that a mixed culture medium containing 30-70 percent of hydrolyzate is inoculated into with the inoculation amount of 5-10 percent for culture for 15-20 hours, and the inoculation into the hydrolyzate with the inoculation amount of 10 percent or simultaneous saccharification and fermentation of the stalks is further carried out for producing the ethanol. The invention has the advantages of being applicable to high-solid-content stalk enzyme hydrolysis and simultaneous saccharification and fermentation, with short fermentation period, high ethanol yield, and low production cost, thereby having great industrial application prospect.

Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production belong to the technical field of biological engineering. The strain is any one strain selected from pichia anomala CGMCC4740, schizosaccharomyces pombe CGMCC4744, zygosaccharomyces bailii CGMCC4745, trichosporon asahii CGMCC4746, saccharomyces cerevisiae CGMCC4747, kazachstania exigua CGMCC4748, pichia fermentans CGMCC4750. The strain is separated from various Daqu, fermented grains, brewing raw materials and brewing environment of several Chinese famous distilleries; the screening method comprises the following steps: diluting distiller yeast samples, fermented grains, brewing raw materials or brewing environment substances, coating on a TTC primary screening plate, selecting a colony with an obvious red color, inoculating the colony in a shake flask for culture, performing secondary screening, determining the contents of ethanol and fusel oil of the fermentation broth by a distillation specific gravity method and HS-SPME-GC-MS respectively, screening the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and brewed wine industry.

The invention discloses a recombinant yeast strain (Saccharomyces cerevisiae)) capable of efficiently metabolizing xylose, which is named SyBE005. The recombinant yeast strain has been collected in Common Microorganism Center of China Committee for Culture Collection of Microorganisms, the collection number is CGMCC No.6634, and the recombinant yeast strain has the capacity of efficiently metabolizing xylose to produce ethanol. The recombinant yeast strain SyBE005 can effectively ferment xylose to generate ethanol. The ethanol yield reaches 64% of the theoretical yield, and the yield of the byproduct xylitol is lower than 12%; and thus, the recombinant yeast strain can efficiently convert xylose into ethanol, and is an excellent strain utilizing cellulose hydrolysate.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention discloses a method for producing ethanol by high-efficiency simultaneous saccharification and cofermentation, which comprises the following steps: pretreating; concentrating and detoxifying; preparing yeast seed solution; performing simultaneous saccharification and cofermentation; and distilling/rectifying. When the method provided by the invention is used, the inhibition action of a monosaccharide component formed by hydrolysis of cellulose on cellulose is relieved, the enzymolysis saccharification efficiency and fermentation yield are improved, the recovery rate of hemicelluloses in bagasse is about 60 percent, the cellulose recovery rate is about 86 percent, and the total ethanol yield of dry bagasse can reach about 20 percent.

Ethanol and furfural challenged strains of E. coli FBR5 exhibiting higher ethanol yield, productivity, and tolerance to both ethanol and furfural than FBR5 and methods for producing same.

Six hop acids are common to hops and beer: alpha acid, beta acids, isoalpha acids, rho-isoalpha acids, tetrahydro-isoalpha acids, and hexahydro-isoalpha acids. The six hop acids were tested to determine which were the most effective in inhibiting the growth of bacteria common to fuel ethanol production. The bacteria used in the tests were Lactobacillus brevis and Lactobacillus fermentum. The minimum inhibitory concentrations (MIC) of the hop acids were determined using MRS-broth. Molasses mash and wheat mashes were used as the growth media for the fermentations. In all cases the hop acids controlled the growth of these two lactobacillus bacteria with tetrahydroisoalpha acid, hexahydroisoalpha acid, and isoalpha acid killing the most bacteria at the lowest MIC. Treating yeast propagators, steep tanks, and fermenters with a minimum inhibitory concentration of hop acids will stop bacteria growth, increase ethanol yields and avoid the need for antibiotics.

A process to improve ethanol yield, decrease fermentation time and reduce byproduct formation by monitoring and controlling oxidation reduction potential (redox) of the fermentor is disclosed.

The invention relates to the ethanol preparation field, and discloses a method for preparing ethanol through tuber crops. The method is based on an ethanol preparation device. The invention is characterized in that: the preparation method comprises the following steps of preprocessing; mixing materials; liquefying; desanding; and generating ethanol. The method arranges the desanding step behind the liquefying step and adopts a three-stage desanding process with reasonable process layout, obvious desanding effect and high ethanol yield.

This invention discloses a method for producing fuel ethanol from a straw fibre raw material, comprising: (1) raw material selection; (2) disintegration; (3) pre-processing; (4) double-enzyme saccharifying; (5) sterilization; (6) enlargement culture and application of Pachysolen tannophilus P-01; (7) fermentation; (8) distillation; characterizing: the pre-processing employs white-rot fungi solid culture to decompose lignin. The procedures comprise: (1) bacteria selection: Phanerochete chrysosporium, which is donated by Shanghai hydnology institute of Chinese Academy of Agricultural Sciences, is a fugus, and can produce lignin decomposing enzymes under specific culture conditions, comprising lignin peroxidase, manganese dependent peroxidase, laccase, and so on; (2) specific culture media producing conditions: i. add 2g glucose, 0.3g ammonium chloride and 100g water to dry straw per 100g and mix homogenously, sterilize at 121Deg C for 2h, inoculate Phanerochete chrysosporium for culture decomposition; ii. conditions of culture decomposition: inoculation 5%, 37-39Deg C, pH normal, material to volume: 1:5 ( dry material weight: container volume), cycle 30 days. This invention can improve the ethanol yield and decease cost.

The invention discloses vanillin-tolerant saccharomyces cerevisiae. The strain is named as saccharomyces cerevisiae EMV-8 and collected in CGMCC on July 16th, 2010 with a collection number CGMCC No.4012. The strain is obtained by domesticating saccharomyces cerevisiae EM-13 in a YEPD liquid culture medium prepared from corn straw steam blackout extraction solution of different concentrations and screening; compared with the original strain EM-13, the strain can consume all glucose faster and has high ethanol yield when cultured in the YEPD liquid culture medium containing 1g/L of vanillin,; when cultured in the YEPD liquid culture medium containing 2g/L of vanillin, the strain has the growth delay period shortened by about 20 hours compared with the original strain; and the strain has good application value for further breeding the inhibitor tolerant saccharomyces cerevisiae in lignocellulose hydrolysis solution, and meanwhile has certain theoretical value for researching the phenolic compound tolerance mechanism of the saccharomyces cerevisiae.

The invention discloses a method for carrying out alkaline pretreatment on plant fiber raw materials for preparing ethanol through enzymolysis and fermentation. According to the method, firstly, green liquid is used for carrying out alkaline treatment on the air dried plant fiber raw materials; then, under the condition that the substrate w/v concentration being 5 to 15 percent, the cellulose is adopted for batch type hydrolysis for 48 to 72 hours; and after the hydrolysis completion, hydrolysate is subjected to solid-liquid separation, the obtained clear liquid is concentrated to a state that the glucose concentration in the sugar liquid is 100 to 200g/L, hexose in the concentrated sugar liquid is converted into the ethanol through brewer's yeast, the ethanol mash is removed through distillation, and then, the pichia stipitis is used for converting pentose into the ethanol. The method realizes the goals that the mature process and equipment of the paper pulp process are utilized for recovering chemical medicine and heat energy, the consumption of chemicals and the loss of heat energy are reduced, the environment pollution is reduced, the clean production of the ethanol is realized, the efficient proceeding of the enzymolysis and the hexose and pentose fermentation are respectively ensured through the low-substrate-concentration enzyme hydrolysis and the sequential fermentation, particularly, through the pentose fermentation, the ethanol yield of each ton of plant fiber raw materials is improved, and the raw material cost of the ton ethanol is reduced.

The present invention provides compositions and methods for producing ethanol wherein the amount of CO₂ by-product is reduced during the fermentation process. The invention includes the use of oxidized lignin during the fermentation process.

The present invention discloses one kind of Saccharomyces cerevisiae strain with glycerin passage protein gene deficiency and resulting in lowered glycerin generation and raised ethanol yield and its construction process. The construction process includes the following steps: 1. obtaining Saccharomyces cerevisiae monoploid and deleting of URA3 gene; 2. constructing PUC18-FPS1-ORF plasmid; 3. constructing PUC18-FUF plasmid; and 4. constructing KAM-21 strain. The present invention deletes glycerin passage protein FPS1 gene code embedded on cell membrane and loses the expression of FPS1 gene by means of gene engineering technology, so as to inhibit glycerin synthesizing way, convert from glycerin metabolism to ethanol metabolism, increase ethanol yield, and solve serial problems in ethanol production.

The invention discloses a method for constructing the ethanol high-yield strains of Saccharomyces cerevisiae, which comprises the following steps: (1) ethylmethane sulfonate is utilized to carry out random mutagenesis to the amphiploid strains of the Saccharomyces cerevisiae to cause the final concentration of the volume of the ethylmethane sulfonate in the bacteria liquid to be processed to be 2 to 4 percent and the concentration of the amphiploid strains of the Saccharomyces cerevisiae to be 2 multiplied by 10<6> to 6 multiplied by 10<6> cell/ml; (2) 3 to 5 rounds of the sexual recombination of high efficient sporulation, spore purification and full integration cause genome recombination between strains in a library to construct the ethanol high-yield strains of the Saccharomyces cerevisiae. The ethanol output of the recombinant strains constructed by the method of the invention is increased by 8.88 percent compared with that of contrast strains, residual sugar is reduced by 64.37 percent, the fermentation cycle is shortened by 10 hours and ethanol and high osmotic resistance are 5.10 times of that of the contrast strains, thus providing excellent strains for the industrial production of fuel ethanol.

The invention discloses a compound enzymatic hydrolysis method for straw raw materials, which comprises: hydrolyzing the straw raw materials by diluted acid to decompose lignin; adding xylanase, cellulose, amylase, pectinase and beta-glucanase, and adjusting the pH value of the mixture to 4.3 to 5.5 by using an acidic matter or alkaline matter; and performing enzymatic hydrolysis at 40 to 55 DEG C for 24 to 72 hours. In the invention, a compound enzyme method is used to perform enzymatic hydrolysis on straw to convert the straw into reducing sugar, a proper mixing ratio of compound enzymes is selected to allow enzymes to interact to maximize the hydrolysis, the concentration of the reducing sugar generated by the enzymatic hydrolysis is high, the yield of sugar is high, the enzymatic hydrolysis conditions are mild, adverse influences on fermentation in a later period are avoided, the growth of a strain for fermentation in the later period is not inhibited, the ethanol yield during fermentation is high, the investment and cost are small, and thus, the method is suitable for large-scale use in industry.

The invention discloses a method for producing high-concentration ethanol by the mixed fermentation of starch and molasses which are used as raw materials. The method comprises the following steps of: adding water to the raw material of starch and stirring to constant volume, liquefying at low temperature and simultaneously fermenting and saccharifying, and then adding the raw material of molasses for mixed fermentation through the batch or continuous flow feeding supplement way. The mixing ratio of the raw materials of starch and molasses is 1:2-8:1 (W/V), the temperature of fermentation is 30-37 DEG C, the time of fermentation lasts for 40-48h, the percentage of ethanol yield is 12.43-16.67% (V/V). The invention changes the traditional single raw material fermentation way, and not only improves the total sugar concentration of the fermented mash, but also reduces the viscosity of starch raw mash and improves the liquidity, so the concentration of ethanol in the fermentation mash is increased, and the raw material utilization rate is improved, and ethanol distillation energy consumption and production cost are decreased, the amount of sewage discharge is reduced, the load of sewage treatment is lowered and the ethanol production process is more economical and environmentally friendly.