4 results about "Xylose fermentation" patented technology

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to a method for promoting the co-utilization efficiency of glucose and xylose of saccharomyces cerevisiae and application of the method. Specifically, it is accidentally found through research that the co-fermentation efficiency of the saccharomyces cerevisiae glucose and xylose can be effectively improved by activating a TORC1 signal channel, and the method is achieved by adding a TORC1 signal channel activator into a fermentation system. And the typical activating agent comprises leucine and glutamine. According to the technical scheme, the xylose fermentation efficiency can be remarkably improved, the overall fermentation time is shortened, a foundation is laid for effective utilization of biomass resources such as lignocellulose, and therefore the method has good practical application value.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant foal foal xylose as well as a construction method and application thereof. The recombinant foal foal capable of improving the bacterial cellulose yield and the xylose utilization amount is constructed by taking the foal foal foal as an original strain and overexpressing a xylose isomerase gene xylA, a xylulokinase gene xylB, a phosphopentose pathway key gene TKL and a structural gene BcsD. Under the fermentation conditions of pure xylose and xylose and glucose, the bacterial cellulose yields of the bacterial strain can reach 9.51 g / L and 8.1 g / L respectively and are increased by 553.23% and 44.75% respectively compared with original bacteria, and the highest xylose consumption of the bacterial strain under the fermentation conditions of pure xylose can reach 11.18 g / L. According to the method, the utilization rate of xylose is remarkably increased, the production efficiency of bacterial cellulose is improved, and a feasible solution is provided for replacing efficient conversion of traditional biomass resources with glucose as a carbon source.

An engineered yeast strain capable of efficient fermentation of xylose to ethanol, and methods of making and using the strain, are provided.

The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases which, upon transformation of a eukaryotic microbial host cell, such as yeast, confer upon the host cell the ability to isomerize xylose into xylulose. The nucleic acid sequences encode xylose isomerases originating from bacteria such as Eubacterium sp., Clostridium cellulosii, and others. The invention further relates to fermentation processes in which the transformed host cells ferment a medium containing xylose to produce ethanol or other fermentation products.