1105 results about "Bacterial strain" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

The invention belongs to the technical field of agricultural sensing data management and control, and particularly relates to a vision-based multi-modal data fusion intelligent monitoring culture method and system, and the method comprises the steps: obtaining the growth image data and environmental parameter data of a strain based on a multi-sensor information source; identifying the growth image data through a deep learning algorithm, and outputting an image identification result; performing fusion processing on the image recognition result and the environment parameter data through a multi-data fusion algorithm to generate a comprehensive environment evaluation index; importing the comprehensive environment evaluation index and the abnormal state information into a growth environment control model, generating an environment regulation and control instruction conforming to a preset strain growth index, and executing the environment regulation and control instruction through an environment regulation and control module; the system can be used for realizing comprehensive environment monitoring, accurately evaluating the strain growth environment condition, and avoiding errors and one-sidedness caused by a single data source, so that culture environment parameters are dynamically adjusted.

The preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO.33629, the preservation date of the bacterial strain is February 24, 2025, the preservation classification of the bacterial strain is Lactobacillus sakei, and the preservation unit of the bacterial strain is China General Microbiological Culture Collection Center. The strain provided by the invention can reduce the concentration of blood uric acid, relieve kidney inflammation caused by hyperuricemia, improve the protein level of a kidney uric acid excretion channel and reduce the expression level of uric acid resorption protein.

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

The invention relates to the field of fermentation processes, in particular to a method for preparing 1, 4-butanediol through multi-strain synergistic fermentation, which comprises the following steps: inoculating a first strain and a second strain into a culture medium containing glucose and / or xylose for synergistic fermentation, producing ornithine by the first strain by taking glucose and / or xylose as a substrate, and converting ornithine into 1, 4-butanediol by the second strain. The invention discloses 1, 4-butanediol. Glucose is converted into ornithine through the first strain, ornithine is converted into 1, 4-butanediol through the second strain, the first strain and the second strain are subjected to synergistic fermentation, preparation of 1, 4-butanediol through microbial synergistic fermentation is achieved, and higher metabolic capacity and higher production efficiency are achieved. The mixed sugar of xylose and glucose prepared from biomass raw materials is used as a substrate for fermentation, so that the method has the advantages that the raw materials are easy to obtain, renewable and low in cost, and the production cost can be reduced.

The invention discloses an algicidal bacterium and application thereof, the algicidal bacterium is named as Bacillus sp.L1.3, and is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025629. The nucleotide sequence of the 16S rRNA gene of the strain is as shown in SEQ ID NO: 1. The invention also provides application of the algicidal bacteria in treatment of water bloom of freshwater cyanobacteria, and the freshwater cyanobacteria is microcystis aeruginosa. Fermentation liquor of the strain L1.3 has a strong inhibition effect on growth of microcystis aeruginosa, secretions of the strain L1.3 can destroy cell walls, cell membranes, thylakoid and other structures of algae cells, inhibit photosynthesis of algae and cause irreversible damage to the algae, and the strain L1.3 has wide-range temperature stability and can be used for preparing the microcystis aeruginosa strain L1.3. The bacterial strain is suitable for being prepared into an algicidal bacterial agent to be applied to the field of biological control of freshwater cyanobacterial bloom.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention relates to the technical field of crop straw treatment and soil improvement, in particular to a rice straw returning method for improving soil fertility, which mainly comprises the following steps: crushing rice straws, soaking with a sodium hydroxide solution, and drying; then selecting specific strains, mixing and fermenting to prepare a microbial agent; preparing a composite additive in proportion; within 1-2 days after the rice is harvested, the pretreated straw, the microbial agent and the composite additive are applied to the field, deep ploughing is conducted, and irrigation is conducted; and finally, performing rotary tillage and soil preparation after straw decomposition, and monitoring and adjusting the pH value of the soil when next-stubble crops are planted. According to the method, straw is pretreated, lignin is destroyed through a sodium hydroxide solution, the particle size is accurately controlled, drying is conducted in cooperation, the microbial decomposition efficiency is improved, straw and soil are fully mixed through accurate ploughing in the field, decomposition is accelerated into nutrients, the straw can be powerfully decomposed through a microbial agent, and the soil micro-ecology is improved; the composite additive supplements various nutrients and enhances the water and fertilizer retention capability of soil.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The invention provides a bifidobacterium longum subsp. Longum aFMT-103 strain, which is classified and named as Bifidobacterium longum subsp. Longum, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 65181. The invention also provides a preparation method of the bifidobacterium longum subsp. Longum aFMT-103 strain. The invention further provides a microbial preparation containing the aFMT-103 bacterial strain. In addition, the invention also provides an application of the strain or the microbial preparation in preparation of an anti-depression product, and also provides an anti-depression product. The bifidobacterium longum subsp. Longum aFMT-103 strain has the advantages that the bifidobacterium longum subsp. Longum aFMT-103 strain is separated from the faeces of healthy adults; the strain has a remarkable anti-depression effect, can be used for preparing a preparation for resisting depression symptoms, and is of great significance in prevention and adjuvant therapy of depression.

The invention provides a strain for enhancing an immune function and application thereof, and belongs to the technical field of microbial medical treatment. The fungus provided by the invention is cordyceps sinensis qinggensis Cs-2025, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.41879. The invention also provides a preparation method of the cordyceps sinensis qinggensis. The strain provided by the invention can be used as a substitute of cordyceps sinensis, has the effect of remarkably enhancing the immune function, and is free of toxic and side effects. The method disclosed by the invention is low in production cost, short in time and easy for large-scale production, and an effective way is provided for solving the problem of shortage of natural cordyceps sinensis resources.

This invention provides a genetically engineered bacterial strain, its preparation method, and its application in the synthesis of perillaldehyde. The genetically engineered bacterial strain includes the CcLPPS gene derived from *Cistus creticus*; the strain is selected from *Yarrowia lipolytica*. This invention constructs a stable, highly efficient de novo perillaldehyde synthesis strain. The entire fermentation process requires no addition of any organic reagents or intermediate substances, significantly reducing production costs and simplifying subsequent separation and purification steps. The engineered *Yarrowia lipolytica* strain provided by this invention exhibits high production intensity and a simple fermentation process, showing promising application prospects and laying the foundation for the microbial fermentation production of perillaldehyde.

The invention discloses streptomyces bulbus as well as a multifunctional fungicide and application thereof. According to the invention, firstly, a strain of Streptomyces tauricus zhanshen-LZ-12 is separated and screened from straw returning soil, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34456. The invention also discloses a preparation method of the Streptomyces tauricus zhanshen-LZ-12. The strain has the functions of degrading cellulose, decomposing protein, fixing nitrogen, resisting salt, degrading phenolic acid autotoxic substances (benzoic acid, p-hydroxybenzoic acid and cinnamic acid) and the like. Bacterial powder obtained after fermentation of the bacterial strain is uniformly mixed with bacillus bacterial powder to obtain the multifunctional bacterial agent, and when straw returning or composting is carried out, the multifunctional bacterial agent is adopted, straw decomposition can be accelerated, soil ecology can be improved, crop growth can be promoted, and the stress resistance of crops can be enhanced.

The present invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation. The low-temperature-resistant multifunctional bacteria is the strain TLT1 of the genus Tularemia, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on August 9, 2023, with the deposit number: CGMCC No: 40754. The bacterial agent of the present invention can be used to stabilize zinc in the soil environment, which helps to enrich the database of bacterial strains that stabilize heavy metals and have the ability to degrade quinolone antibiotics. The present invention prepares the purified strain into a bacterial suspension, which can effectively stabilize zinc in different environmental media such as water and soil under low-temperature conditions, and has the ability to degrade enrofloxacin in different environmental media such as water and soil. Compared with methods such as chemical oxidation, this method is more efficient, less expensive, and has no secondary pollution, providing an effective biological-non-biological combined remediation technology for the remediation of zinc-enrofloxacin complex-contaminated soil and water.

The invention relates to the technical field of biology, and particularly discloses a strain with a uric acid reducing characteristic and a growth-dependent uric acid characteristic, and the microbial preservation number of the strain is GDMCC No.65914. The uric acid reducing strain is screened from pickles by a transparent ring method, the 16S rRNA gene sequence of the uric acid reducing strain is studied, a resource library of the uric acid reducing strain is expanded, resources are provided for research and development of uric acid reducing drugs in the clinical field, non-target strains are effectively removed through a dual specific screening mechanism of uric acid dependent growth verification and liquid fermentation induced uric acid reduction, and the yield of the uric acid reducing strains is increased. The uric acid reduction and uric acid dependence function stability and metabolic specificity of the screened strain are ensured, the high uric acid reduction strain obtained through screening can be directly used for developing a novel microecological preparation or a uric acid reduction preparation, and compared with traditional chemical drugs, the high uric acid reduction strain has the advantages of being high in safety, good in biocompatibility and small in side effect; high-quality strain resources are provided for biological treatment of hyperuricemia and gout and development of uric acid detection reagents.

The object of the present invention is to use the strain of bacteria named Lacticaseibacillus paracasei DG® CNCM I-1572 DSM 34154 for use in a method of treating dysbiosis, particularly in patients with IBS (Irritable Bowel Syndrome), preferably without constipation, who have high fecal levels of Collinsella aerofaciens.

The invention discloses a bacterial synbiotic beneficial to growth promotion of pasture in saline-alkali soil and application of the bacterial synbiotic. The bacterial synbiotic is a combination of bacterial strains NAU-RHO, NAU-MIC2 and NAU-FLA4 with 4-guanidinobutyric acid. The bacterial strains NAU-MIC2, NAU-FLA4, NAU-RHO are respectively classified and named as microbacterium laeviniform, Flavobacterium anhuiense and Rhodococcus oxyrhenium, and the research result shows that the bacterial synbiotics can be used for improving the salt tolerance and growth promoting effects of the bacteria on the medicago sativa and promoting the high yield of the medicago sativa in the saline-alkali soil.

The invention discloses a microbial composite flora, a microbial agent and application of the microbial composite flora and the microbial agent, and relates to the technical field of microorganisms and application of the microorganisms. The invention relates to a microbial composite flora, which is characterized in that the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12, and the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12. The microorganisms for constructing the composite flora are separated from the semi-coke wastewater, have better phenol resistance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biologically removing nitrogen and phosphorus and degrading COD. Under the condition that the original microbial community composition in the wastewater is not changed, the microbial compound flora can effectively improve the degradation rate of COD (Chemical Oxygen Demand) in the phenol-ammonia wastewater and effectively reduce the treatment cost of high-phenol-ammonia wastewater such as semi-coke and the like through the synergistic effect of the strains, and has great potential in the aspect of promoting the low-carbon green sustainable development of the coal chemical industry.

The invention discloses a Pseudomonas nicosulfurones bacterial strain and an application of the Pseudomonas nicosulfurones bacterial strain in degradation of phenanthrene and benzopyrene. According to the invention, a degradation strain Pseudomonas nicosulfurensis DY-8, which takes phenanthrene and benzopyrene as carbon sources, is obtained by domestication, separation and identification from petroleum-polluted soil in Ningbo. The invention also relates to a method for preparing the degradation strain Pseudomonas nicosulfurensis DY-8 with phenanthrene and benzopyrene as carbon sources. The DY-8 can utilize phenanthrene and benzopyrene as carbon sources, and after the DY-8 is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rates of phenanthrene and benzopyrene can reach 70% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

The present invention relates to recombinant virulence attenuated Gram-negative bacterial strains and its use in a method of treating cancer in a subject.

The present invention relates to a fermentation product obtained by a fermentation process of at least one bacterial strain in the presence of at least one vegetable extract. The fermentation product of a bacterial may comprise alimentary and / or pharmaceutically acceptable components or comprised in medical food or nutritional composition. The fermentation product and is useful for the treatment and / or prevention of inflammatory diseases, in particular, inflammatory bowel disease.

The invention provides a single bacterium drug sensitivity detection method and system, and relates to the technical field of drug sensitivity detection.The single bacterium drug sensitivity detection method comprises the steps that a cultured to-be-tested strain is subjected to fluorescent staining and then mixed with an antibiotic solution, and a mixed solution is obtained; continuing the strain to be tested in the mixed solution through a structured light illumination microscope to obtain an original imaging sequence; reconstructing and preprocessing each original imaging sequence to obtain a processed imaging sequence, and then cutting out a single bacterium to be tested from each processed image in the processed imaging sequence to obtain a plurality of region-of-interest sequences; and inputting each region-of-interest sequence into a pre-trained evaluation model to obtain a detection result including drug resistance or susceptibility. The method has the beneficial effects that the method focuses on the early change of a subcellular structure before bacterial division, so that the detection time is greatly shortened; imaging acquisition and image processing and cutting are carried out on the single bacterium level, and the survival (drug resistance prompting) state and the non-survival (susceptibility prompting) state of single bacteria can be rapidly distinguished.

The invention discloses a high-yield lycopene recombinant rhodobacter sphaeroides strain as well as a construction method and application thereof, and belongs to the technical field of biosynthesis and biological energy sources, the construction method comprises the following steps: knocking out a crtC gene of rhodobacter sphaeroides by using a homologous recombination method to obtain a recombinant strain RS01; the method comprises the following steps: on the basis of a strain RS01, replacing a crtI3 gene on a rhodobacter sphaeroides genome with a crtI4 gene (from rhodospirillum rubrum) by using a homologous recombination method to obtain a recombinant strain RS02; the method comprises the following steps: connecting crtB and crtE genes to a pYYDT vector to construct a plasmid pYYDT-crtB-crtE, transferring the plasmid pYYDT-crtB-crtE into escherichia coli S17, and then introducing the plasmid pYYDT-crtB-crtE into a recombinant rhodobacter sphaeroides RS02 in a conjugational transfer manner to obtain a recombinant strain RS03. The recombinant rhodobacter sphaeroides constructed by the invention can be used for efficiently producing lycopene.

The invention relates to a cellulose microbacterium J6, the preservation number of which is CCTCC (China Center For Type Culture Collection) NO: M2025107, and the bacterial strain has the capability of degrading cellulose, protein and starch, is acid-resistant, alkali-resistant, salt-resistant and heavy metal ion-resistant, and can grow in a temperature range of 10-50 DEG C. The invention also discloses an application of the strain in aerobic composting, and the strain can effectively promote a compost body to enter a high-temperature stage more quickly, accelerate the composting process, shorten the decomposition period and improve the decomposition degree.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

The invention discloses a conyza sumen biocontrol bacterium and application thereof, and relates to the technical field of weed biological control, the conyza sumen biocontrol bacterium has a strain number of SMBJC-3, is preserved in the China Center for Type Culture Collection, the preservation time is September 17, 2025, the preservation number is CCTCC NO: M 20252054, and the conyza sumen biocontrol bacterium is classified and named as Colletotrichum orbiculare. The conyza sativa biocontrol bacterium provided by the invention aims to provide a new resource and method for biological control of conyza sativa.

The invention discloses bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in promoting growth of plants in salinized land. The strain is preserved in Guangdong Microbial Culture Collection Center on June 24, 2025, and the preservation number is GDMCC NO: 67014. The bacillus amyloliquefaciens R1 obtained by screening is a high-yield IAA (Indoleacetic Acid), is a bacterial strain capable of remarkably promoting the growth of plants in the salinized land, and has good development potential and application value for promoting the growth of the plants in the salinized land. The problem that in the prior art, microbial strains with the rhizosphere growth-promoting and saline-alkaline-tolerant functions are lacked is solved.

The invention discloses a bacterial strain C5A-1 capable of producing lipopeptide and having a plant growth promoting function and application of the bacterial strain C5A-1, solves the technical problem of promoting plant growth, especially root length, plant height, stem diameter, leaf number, leaf area, underground dry weight and overground dry weight, and particularly discloses bacillus velezensis C5A-1 or a variant or a progeny thereof. The bacillus velezensis C5A-1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32880. When the bacteria or the fermented supernate thereof or the combination thereof is applied to plants, the growth of the plants can be promoted, and the bacteria can be used for industrial production.