Schizochytrium limacinum and method or fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of schizochytrium limacinum

A technology of high-density fermentation and Schizochytrium, which is applied in the application field of marine microorganisms, can solve the problems of excessive fishing, reduced content, difficult purification process, etc., and achieves the effect of improving price advantages.

Active Publication Date: 2013-01-23
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF4 Cites 61 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional raw material for the production of DHA is mainly fish oil, but the production of DHA through fish oil has the following disadvantages: (1) fish oil resources are limited, the output is unstable, and the output and quality of fish oil fluctuate greatly, which is far from meeting the market demand
(2) The DHA content in fish oil is not high, accounting for only 7% to 14%, and it is difficult to separate from a large amount of EPA and other highly unsaturated fatty acids with similar structures (3) The purification process is complicated, the production cost is high, and the product yield Low
In the actual production process, omega-3 polyunsaturated fatty acids are hydrogenated and saturated, reducing their content in fish oil, resulting in waste of raw materials, and also impairing the quality of DHA and EPA (4) Fish oil is easy to oxidiz

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Schizochytrium limacinum and method or fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of schizochytrium limacinum
  • Schizochytrium limacinum and method or fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of schizochytrium limacinum
  • Schizochytrium limacinum and method or fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of schizochytrium limacinum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Effects of Different Carbon Sources and Carbon Source Concentrations on the Growth and Oil Accumulation of Schizochytrium SD116 Strain

[0032] In a 250ml Erlenmeyer shaker flask, configure 50ml of medium, the nitrogen source is yeast extract 20g / L, the salinity is 15, add different carbon sources (glycerol, glucose, fructose, xylose, sucrose, maltose and starch) , the concentration is 60g / L, the pH value is adjusted to 6.0, after autoclaving, insert 5ml of the pre-cultivated strain seed solution, and vibrate at 25°C for 5 days on an air bath shaker, and the shaking speed is 180rpm. The bacteria were collected by centrifugation, freeze-dried to constant weight, and their dry weight was measured; some of the bacteria were taken, and the oil was extracted and methylated by the conventional chloroform-methanol fermentation method, and the percentage content of DHA in the bacteria was determined by GC-MS. The results are shown in Table 1

[0033]After determinin...

Embodiment 2

[0041] Example 2: Effects of Different Nitrogen Sources and Nitrogen Source Concentrations on the Growth and Oil Accumulation of Schizochytrium SD116 Strain

[0042] In a 250ml Erlenmeyer shaker flask, configure a 50m culture medium, use glucose as a carbon source, a concentration of 60g / L, and a salinity of 15, add organic nitrogen sources (yeast extract, peptone and tryptone) with a concentration of 20g / L respectively and 5g / L inorganic nitrogen source (urea, ammonium acetate and sodium nitrate), adjust the pH value to 6.0, after autoclaving, insert 5ml of pre-cultivated strain seed liquid, and vibrate on an air bath shaker at 25°C For 5 days, the shaking speed was 180 rpm. The analysis method is the same as above, and the experimental results are shown in Table 3.

[0043] Table 3 Effects of different nitrogen sources on the growth and oil accumulation of Schizochytrium SD116 strain

[0044]

[0045] It can be seen from Table 3 that yeast extract and peptone can better...

Embodiment 3

[0050] Example 3: Effects of Different Temperatures and Salinity on Growth and Oil Accumulation of Schizochytrium SD116 Strain

[0051] Schizochytrium growth and oil accumulation are affected by temperature and salinity. The experiment adopts optimized carbon source, nitrogen source and concentration, and designs different temperatures (20-37° C.) and salinity (0-60). The experimental method and analysis method are the same as the above examples.

[0052] Table 5 Effects of different temperatures on the growth and oil accumulation of Schizochytrium SD116 strain

[0053]

[0054]

[0055] It can be seen from Table 5 that the lower the temperature, the higher the DHA content of the Schizochytrium SD116 strain, indicating that low temperature is conducive to the accumulation of DHA; when the temperature is 25-28 °C, the growth of the SD116 strain is the largest, reaching 29.56 g / L, but when the temperature was higher than 30℃, the biomass and oil content of Schizochytrium ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of microbial fermentation engineering, and discloses schizochytrium limacinum (Aurantiochytrium sp.SD116). The schizochytrium limacinum is stored in China Microbial Culture Collection Administration Committee General Microbial Center with a number of CGMCC No: 6208), and a method for fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of the schizochytrium limacinum. According to the schizochytrium limacinum and the method disclosed by the invention, a bacterial strain fermenting condition is optimized based on element supply and a fermenting control angle, a carbohydrate supplementation operation is carried out, therefore, high-density fermentation is achieved, dry cell weight finally reaches 70.43g/L, grease content reaches 50.1g/L, DHA occupies more than 35% of total content of fatty acid; moreover, bioactive substances such as beta-carotene, astaxanthin and squalene are contained. The complete set of technology is convenient to operate; higher biomass liveweight and DHA content can be obtained; fermenting cost can be reduced; and the method is suitable for industrial fermenting production.

Description

technical field [0001] The invention relates to the application technology of marine microorganisms, in particular to a marine fungus Schizochytrium SD116 strain and a method for producing DHA by high-density fermentation thereof. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 polyunsaturated fatty acid, which has important physiological functions such as promoting brain cell development, lowering blood fat, protecting eyesight, anti-cancer and improving immunity, and is widely used in infants. Children's food and pharmaceutical industry. In addition, DHA is also an essential fatty acid required for the growth and development of various seawater fish, which can improve the survival rate of fry and reduce the incidence of albinism. [0003] The traditional raw material for the production of DHA is mainly fish oil, but the production of DHA through fish oil has the following disadvantages: (1) fish oil resources are limited, the output is uns...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 崔球高莽宋晓金冯银刚
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap