177 results about "Squalene" patented technology

The invention belongs to the technical field of biological medicine, and particularly discloses a composite vaccine adjuvant as well as a preparation method and application thereof. The composite vaccine adjuvant provided by the invention is an oil-in-water emulsion, and simultaneously contains squalene, polysorbate 80, sorbitan trioleate, polyinosinic acid (Poly I: C) and CpG oligodeoxynucleotide (CpG-ODN). The sequence-optimized CpG-ODN and Poly I: C are used in a specific emulsion system in a combined mode, a remarkable synergistic immune enhancement effect can be generated, the induced humoral immunity and cellular immunity response level is remarkably higher than the sum of the effects when all the components are independently used, and the composite adjuvant is good in stability, high in immunostimulatory activity and suitable for clinical application. The immune effect can be remarkably improved by combined application with various vaccine antigens. The invention also provides a preparation method and application of the composite vaccine adjuvant.

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The invention discloses an emulsifier for preparing a Pickering emulsion, the Pickering emulsion as well as a preparation method and application of the Pickering emulsion. The emulsifier of the Pickering emulsion is obtained by dissolving distearoyl phosphatidylcholine, cholesterol and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 in ethanol, carrying out rotary evaporation to remove ethanol, then adding an antigen aqueous solution containing an antigen, continuing rotary evaporation to obtain liposome nanoparticles, heating the liposome nanoparticles with mannose in a water bath, and carrying out freeze drying. The Pickering emulsion is obtained by dispersing an emulsifier in water as a water phase, taking squalene as an oil phase, mixing the water phase and the oil phase, and homogenizing. The Pickering emulsion disclosed by the invention is prepared by taking antigen presenting cell targeted liposome nanoparticles as a raw material; and the antigen has excellent ion concentration characteristic, pH stability, temperature stability and storage stability, and can protect the integrity of the antigen.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention discloses a self-emulsifying adjuvant of a vaccine and a preparation method of the self-emulsifying adjuvant, and relates to the technical field of vaccine adjuvants. The adjuvant comprises an oil phase, an emulsifier and a freeze-drying protective agent, the oil phase comprises squalene and tocopherol, the emulsifier comprises polysorbate and a polyoxyethylene-polyoxypropylene block copolymer, and the freeze-drying protective agent comprises sugar and sugar alcohol. The preparation method comprises the following steps: emulsifying an oil phase and a water phase to form a nano-emulsion, and then freeze-drying to obtain freeze-dried powder. The adjuvant has the advantages of small particle size, uniform distribution, high stability, rapid reconstruction and the like, and can effectively maintain active components and improve the immune effect of vaccines.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention relates to the technical field of synthetic biology, in particular to a squalene high-yield saccharomyces cerevisiae engineering bacterium and a construction method and application thereof.Saccharomyces cerevisiae CEN.PK.2-1D is used as an original strain, pentose phosphate pathway genes ZWF1, GND1, TAL1 and TKL1 and IDP1 genes of TCA circulation are integrated in a genome of the original strain through homologous recombination, and the squalene high-yield saccharomyces cerevisiae engineering bacterium is obtained. Constructing a dual-channel cofactor supply system to improve the intracellular NADPH level and the NADPH / NADP + ratio; according to the saccharomyces cerevisiae genetic engineering strain Sc-Sq04 constructed by the method disclosed by the invention, the yield of squalene obtained by shake flask fermentation reaches 741.08 + / -28.9 mg / L by further integrating all genes of an MVA way, ERG20 and ERG9 genes, and a new thought is provided for transforming saccharomyces cerevisiae to synthesize terpene compounds and promoting industrial application.

Disclosed herein are reagents, reagent kits, and culture media for activating and expanding immune cells in vitro. In some embodiments, the reagents, reagent kits, and culture media comprise a Quillaja saponin. In other embodiments, the reagents, reagent kits, and culture media comprise a squalene-based emulsion. Also disclosed are methods for activating and expanding immune cells in vitro using the reagents, reagent kits, and culture media.

SILICONE-FREE COSMETIC COMPOSITIONS This disclosure relates to compositions including squalene, at least one bio-based branched hydrocarbon, at least one bio-based pentaerythrityl ester, and processes for using such compositions. The cosmetic composition is silicone-free. The cosmetic composition spreads easily and has favorable sensory properties without the use of silicones. Figure for the abstract: none

The present invention relates to a process for obtaining minor components from vegetable oils, and wherein the vegetable oil includes palm oil, palm olein, palm mid-fraction or mixtures thereof and wherein the minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, a fractionation in short path evaporator followed by a further fractionation in short path evaporator. The feed stream may be a condensate stream from a deodorization step.

The present application relates to oxidized squalene cyclase, recombinant vector, recombinant engineering bacteria and application thereof, and preparation method of parkol. The oxidized squalene cyclase comprises: a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1; or a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1 with one or more amino acids being deleted, substituted or added; or a polypeptide having at least 80% homology with the polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1. The above-mentioned oxidized squalene cyclase can catalyze 2, 3 oxidized squalene to produce parkol, and the enzyme can be expressed according to the coding sequence of the cyclase, and then the substrate is catalyzed to improve the yield of parkol.

The invention relates to a squalene epoxidase mutant and application thereof. The amino acid sequence of the squalene epoxidase mutant is an amino acid sequence obtained by deleting amino acids from the first site to the 55th site at the N end of an amino acid sequence of wild squalene epoxidase SgSQE1 from momordica grosvenori, and then connecting a dissolution-promoting tag sequence to the N end and / or the C segment. The squalene epoxidase mutant provided by the invention can be used for remarkably improving the yield of 2, 3, 22, 23-diepoxy squalene.

The present disclosure relates to microbial cells, engineered enzymes, and methods for producing mogrol or mogrosides. The methods include providing a microbial cell that produces squalene and expresses a biosynthetic pathway that converts squalene to the mogrol or mogrosides, and culturing the microbial cell under conditions suitable for producing the mogrol or mogrosides. In various embodiments, the methods disclosed herein produce squalene derivatives in which the extrapathway products cucurbitadienols, hydrolyzed 2,3;22,23-dioxidosqualene, and glycosylated 24,25-dihydroxycucurbitadienols are less than about 40% by weight.

The present disclosure relates to compositions comprising squalene, at least one bio-based branched hydrocarbon, at least one bio-based pentaerythritol ester, and methods of using such compositions. The cosmetic composition is free of silicone. The cosmetic composition is easy to smear and has good sensory properties even without using silicone.

The invention discloses an immunogenic composition containing gE nanoparticles as well as a preparation method and application thereof, and relates to the technical field of biomedicine, the immunogenic composition comprises self-assembled gE nanoparticles and an oil-in-water emulsion adjuvant, and a self-assembled gE nanoparticle antigen comprises gE, a connecting peptide SGS and a gI polypeptide sequence containing Th epitopes; the oil-in-water type emulsion adjuvant is prepared from squalene, Tween 80, DL-alpha-tocopherol and / or Span 85. The immunogenic composition provided by the invention can be applied to vaccines for preventing varicella and / or herpes zoster and / or postherpetic neuralgia, so that the aims of improving the immunogenicity of the gE antigen and reducing side effects of the vaccines are fulfilled.

The invention provides a construction method of a multi-gene synergistic expression vector and application of the multi-gene synergistic expression vector in improvement of oil tea squalene synthesis, and belongs to the technical field of plant genetic engineering and metabolic engineering. MAPK, PP1 and HMGR gene segments are obtained through an RT-PCR technology, the MAPK, PP1 and HMGR gene segments are cloned to expression cassettes containing independent promoters and terminators respectively, and after Insulator isolation elements are inserted, the expression cassettes are integrated to a pCAMBIA1300 vector through a modular assembly technology. Wherein the HMGR gene contains S47A and T208A mutation sites so as to enhance the enzyme activity. The vector constructed by the invention can cooperatively express a target gene in plant cells, so that accurate regulation and control on the phosphorylation state of HMGR are realized. When the method is applied to tobacco or camellia oleifera callus, the squalene content is effectively increased, the method has the advantages of multi-target regulation and control, efficient and flexible construction, high universality and the like, and an efficient tool is provided for anabolism enhancement of natural products.

The invention provides camellia oil containing nano squalene and a preparation method thereof, and relates to the technical field of food processing, in particular to camellia oil containing nano squalene. According to the scheme, a high-pressure homogeneous emulsification technology and a lipid-surfactant synergistic stabilization mechanism are adopted; the preparation method comprises the following steps: firstly, beeswax (solid lipid), camellia oil and rice oil (liquid lipid) are fused at high temperature to form a uniform oil phase; the interfacial tension is reduced through high-speed stirring, in the high-pressure homogenizing process, lipid liquid drops are broken to nanoscale through mechanical shearing force and the hole effect, and soybean lecithin molecules rapidly wrap new liquid drops to prevent aggregation. And finally, quickly cooling to recrystallize and solidify the beewax, wherein a crystalline region of the beewax can enhance the rigidity of emulsion droplets, lock a nano structure and uniformly disperse squalene to obtain the nano functional camellia oil. Solid lipid of the nano-carrier can form a physical barrier, the contact rate of squalene and oxygen is reduced, the oxidation rate is reduced, and the preparation method is simple in process, high in operability and suitable for large-scale mass production.

The invention belongs to the technical field of biological medicine, and particularly relates to a small-molecule exosome-loaded composition based on bone marrow mesenchymal stem cells and application of the small-molecule exosome-loaded composition. The invention discloses the treatment effect of squalene on osteoarthritis for the first time, and further prepares the squalene-loaded bone marrow mesenchymal stem cell exosome. An in-vitro cell experiment and a model animal experiment prove that the composition has a synergistic treatment effect on osteoarthritis. In-vitro tests prove that the squalene-loaded mesenchymal stem cell exosome can improve the survival rate of inflammatory cartilage cells, inhibit release of proinflammatory factors and restore expression of repair factors. Model animal experiments prove that the squalene-loaded mesenchymal stem cell exosome has the effects of resisting inflammation, easing pain and protecting cartilage in the treatment of osteoarthritis.

This invention pertains to modern agriculture, specifically the application of transcription factors CdZF-HD1 / 9 in regulating squalene synthesis. Using the CdSQS promoter as bait, this invention screens a yeast single-hybrid library to identify ZF-HD transcription factors that interact with the CdSQS promoter. ZF‑HD1 and ZF‑HD9 .clone ZF‑HD1 and ZF‑HD9 The nucleotide sequence was further confirmed by dual-luciferase assay and EMSA. ZF‑HD1 and ZF‑HD9 Interacts with CdSQS. ZF‑HD1 and ZF‑HD9 Genetic transformation of Camellia oleifera from Vietnam resulted in increased expression levels of squalene synthase and higher squalene content in the induced hairy roots. This invention provides a scientific basis for finding optimal genetic modification and exogenous regulation methods to increase squalene content in Camellia oleifera, and offers important theoretical and practical support for promoting the development of the Camellia oleifera industry in Hainan.

The invention discloses a preparation method and application of nanoparticles based on anthocyanin coating, and the preparation method comprises the following steps: firstly, extracting anthocyanin and extracellular vesicles (EVs) from lonicera caerulea to obtain anthocyanin powder; then adding PBS (Phosphate Buffer Solution) and squalene into the obtained anthocyanin powder to obtain squalene-anthocyanin; and adding the EVs into squalene and anthocyanin, co-incubating, and carrying out centrifugal treatment, so as to obtain the anthocyanin-coated nanoparticles. The nanoparticle has the characteristics that the particle size is 180-220 nm, the Zeta potential is-45 mV to-55 mV, and the encapsulation efficiency is greater than or equal to 85%, has excellent stability and biocompatibility, has the functions of regulating intestinal flora, resisting depression, resisting oxidation, resisting inflammation and the like, is good in biocompatibility and low in toxicity, solves the problems of stability and bioavailability of the nanoparticle, realizes accurate regulation and control of the intestinal flora, and has a good application prospect. And a safe and efficient new way is provided for treating depression.

The invention belongs to the technical field of edible plant blend oil, and discloses plant compound oil for protecting heart and blood vessels, reducing blood fat, resisting aging and fading spots and a preparation method thereof. The plant compound oil is prepared by compounding camellia oil, fermented soya beans, walnut oil, linseed oil, perilla oil, peach kernel oil, bitter apricot kernel oil, safflower seed oil and semen brassicae oil, and is prepared by the following steps: cleaning raw materials, alternately sterilizing and killing by ultraviolet rays and ozone, squeezing at low temperature, soaking the fermented soya beans at constant temperature, and stirring and mixing in vacuum. The product is rich in active ingredients such as unsaturated fatty acid, polyphenol, flavone, vitamin E, squalene and phytosterol, is high in cooking stability, has a remarkable synergistic effect of all the components, can effectively reduce triglyceride, regulate blood fat and protect heart and blood vessels, and has the effects of resisting oxidation, resisting ageing and fading spots. The process is mild, natural, safe and suitable for industrial production, and the obtained compound oil can be used as functional cooking oil and can also be used for preparing related functional food or health care products.

The application discloses a method for separating squalene from a plant oil deodorization distillate, which comprises mixing the plant oil deodorization distillate containing squalene with a first solvent to obtain a raw material liquid, and performing fractional extraction on the raw material liquid by taking a second solvent as an extractant and a third solvent as a washing agent, wherein the first solvent is selected from one or more of non-polar solvents and weakly polar solvents, the second solvent is selected from one or more of polar solvents, and the third solvent is selected from one or more of non-polar solvents and weakly polar solvents. The fractional extraction method has the advantages of large processing capacity, high product purity and recovery rate, low energy consumption and cost, easy amplification and the like, and is suitable for industrial production.

The application belongs to the technical field of biological medicine, and particularly relates to an adjuvant influenza vaccine and a preparation method and application thereof. The adjuvant influenza vaccine comprises an influenza virus monovalent stock solution, an adjuvant and a PBS buffer solution; the adjuvant is prepared by using mixed oil and an auxiliary agent, the mixed oil comprises squalene, medium-chain triglyceride and alpha-tocopherol; and the auxiliary agent comprises sorbitan trioleate Span-85, Tween 80 and vitamin E succinate polyethylene glycol ester. The adjuvant influenza vaccine prepared by the application has good immunization effect, and can especially improve the overall effect of a tetravalent adjuvant influenza vaccine on four viruses.

The invention relates to the field of bioengineering and technology, in particular to an engineering bacterium for synthesizing isoglycyrrhetinic acid, a construction method of the engineering bacterium and a method for synthesizing the isoglycyrrhetinic acid by using the engineering bacterium. The engineering bacterium comprises introduced exogenous genes, the exogenous genes comprise an HMG-CoA reductase gene, a beta-amyrin synthase gene, a beta-amyrin C-11 oxidase coding gene, a P450 reductase gene, a 11-O-beta-amyrin C-29 oxidase coding gene and an expression-enhanced endogenous gene, and the expression-enhanced endogenous gene is a beta-amyrin synthase gene, a beta-amyrin C-11 oxidase coding gene and a P450 reductase gene. Wherein the endogenous genes comprise squalene oxidase coding genes, the endogenous genes GAL80, HO and BTS1 in the engineering bacteria are knocked out, and the starting strain of the engineering bacteria is saccharomyces cerevisiae. According to the engineering bacterium provided by the embodiment of the invention, isoglycyrrhetinic acid can be obtained in an efficient, green and sustainable production mode.

The invention discloses a pichia pastoris recombinant strain for producing astaxanthin as well as a construction method and application of the pichia pastoris recombinant strain. According to the method, pichia pastoris serves as an operation platform, astaxanthin serves as a target product, a beta-carotene high-yield strain is obtained by increasing the dosage of beta-carotene synthesis genes, then astaxanthin is synthesized through pichia pastoris by integrating astaxanthin synthetase genes, and the astaxanthin yield is improved through optimization of a carotenoid synthesis module. In addition, the synthesis of the recombinant strain astaxanthin is promoted through multienzyme assembly of key nodes in an upstream path of an astaxanthin synthesis path and weakening of a squalene synthesis path in a branch path. On the basis, recombination strain cofactor synthesis is optimized, astaxanthin synthesis is improved, the astaxanthin yield is further increased, and the astaxanthin high-yield strain is obtained. According to the invention, the yield of astaxanthin reaches 4.75 g / L by using a pichia pastoris combined regulation strategy, which is the highest level in current microbial synthesis.

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing squalene by inducing schizochytrium limacinum through a salt stress strategy and application, the method comprises the following steps: fermenting a schizochytrium limacinum strain for fermentation for 0-72 hours, adding 3g / L of NaCl into a fermentation medium, after 72 hours, adding NaCl into the fermentation liquid to 5g / L, continuing to ferment for 120 hours, measuring the sugar consumption every 12 hours in the fermentation process, and supplementing glucose to 80g / L. According to the method, the squalene yield of schizochytrium limacinum is remarkably increased through a salt stress strategy, an expensive inducer is prevented from being used, and the production cost is effectively reduced. 3 g / L of low-concentration salt is added in 0-72 h of the early stage of fermentation to serve as an adaptation period, so that schizochytrium limacinum grows rapidly and is induced to start an osmotic regulation mechanism, the concentration of NaCl is increased to 5 g / L in 72 h, at the moment, the schizochytrium limacinum enters a lipid accumulation period, and high-concentration NaCl is utilized to stress the schizochytrium limacinum to efficiently accumulate squalene.

To provide a new composition for suppressing the accumulation of glycated proteins.SOLUTION: Provided is a composition for suppressing the accumulation of glycated proteins, the composition containing squalene as an active ingredient.SELECTED DRAWING: None

The invention belongs to the technical field of biological medicines, and particularly relates to an adjuvant influenza vaccine as well as a preparation method and application thereof. The adjuvant influenza vaccine comprises an influenza virus monovalent stock solution, an adjuvant and a PBS (Phosphate Buffer Solution), the adjuvant is prepared by using mixed oil and an auxiliary agent, wherein the mixed oil comprises squalene, medium chain triglyceride and alpha-tocopherol; the auxiliary agents comprise sorbitan trioleate Span-85, Tween 80 and vitamin E succinic acid polyethylene glycol ester. The prepared adjuvant influenza vaccine is good in immune effect, and especially the overall effect of the tetravalent adjuvant influenza vaccine on four viruses can be improved.

The invention relates to a derivative of targeted squalene epoxidase as well as a preparation method and application thereof, in particular to application of thiazole imine-containing compounds or agrochemically acceptable salts thereof in prevention and treatment of plant fungal diseases, and part of the compounds show relatively good agricultural bactericidal activity and can be used as potential commercial products.