43 results about "Squalene" patented technology

The present invention relates to a process for obtaining minor components from vegetable oils, and wherein the vegetable oil includes palm oil, palm olein, palm mid-fraction or mixtures thereof and wherein the minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, a fractionation in short path evaporator followed by a further fractionation in short path evaporator. The feed stream may be a condensate stream from a deodorization step.

The application discloses a water-in-oil-in-water adjuvant composition and a preparation method and application thereof, and belongs to the technical field of biological pharmacy. The adjuvant composition is composed of squalene, Tween-80, Span 85 and physiological saline or a phosphate buffer, wherein the volume percentage of squalene is 1% to 6%, the volume percentage of Tween-80 is 0.5% to 3%, the volume percentage of Span 85 is 0.5% to 3%, and the rest is solvent. The composition is prepared into uniform multiple emulsions with a particle size of 40 to 220 nm through high-speed shearing and high-pressure homogenization. The adjuvant can significantly enhance the humoral immune response of various vaccines, and the antibody titer is increased by 4 to 16 times compared with traditional aluminum adjuvant, meanwhile, the adjuvant has low local irritation and good safety, can be stored for more than 24 months at 2 to 8 DEG C, and is suitable for large-scale production.

This invention relates to a monk fruit residue extract containing multiple active ingredients, comprising the following components: 10ppm-20wt% squalene, 1ppm-10wt% monk fruit glycosides, 10ppm-20wt% linoleic acid, 10ppm-20wt% sitosterol, and excipients. This invention fully utilizes monk fruit residue, resulting in an extract rich in various economically valuable active ingredients, with high yields of each. The process is continuous, the equipment is simple, the operation is highly operable, and the production cost is low, making it suitable for industrial production and achieving comprehensive utilization of monk fruit residue resources.

The invention provides ski wax compositions comprising a lipid component which is a cholesterol or a cholesterol derivative, a fatty acid or salt of a fatty acid, a diglyceride, or a combination thereof. It further provides ski wax compositions comprising squalene or a squalene derivative. The ski wax compositions according to the invention can be used as a glide wax or grip wax and may be applied directly to the base of a ski or to a ski skin.

This invention discloses an enzyme preparation and a method for preparing squalene, belonging to the field of genetic engineering technology. The enzyme preparation includes inositol-2-dehydrogenase and squalene dehydrogenase. The amino acid sequence of inositol-2-dehydrogenase is shown in SEQ ID NO. 6, and the amino acid sequence of squalene dehydrogenase is shown in SEQ ID NO. 8 or SEQ ID NO. 10. This invention mutates wild-type inositol-2-dehydrogenase and wild-type squalene dehydrogenase to obtain specific amino acid sequence mutants: inositol-2-dehydrogenase mutant D270P, squalene dehydrogenase mutant H89L, and squalene dehydrogenase mutant H89L / A294R. These mutants exhibit higher enzyme activity, effectively improving the yield and conversion efficiency of squalene during preparation.

The application discloses a kind of squalene continuous flow catalytic hydrogenation squalane application alumina ball load palladium catalyst and its preparation method, the catalyst with gamma-alumina ball as carrier, modification impregnation method load palladium active component, with auxiliary control interface effect, form high dispersion Pd / Al2O3 Catalytic system, palladium loading is 0.01wt%-5wt%.The catalyst is filled in fixed bed reactor, and is applied to squalene continuous flow hydrogenation preparation squalane reaction, using continuous flow hydrogenation process, under mild conditions, squalene can be realized efficient conversion, conversion rate is ≥99%, squalane selectivity is ≥99%.The present application solves the problems of low selectivity, low utilization rate of noble metal and poor stability of existing squalene hydrogenation catalyst, and the problems of low efficiency and difficult industrial continuous production of traditional batch hydrogenation process.The process conditions are mild, environmentally friendly and suitable for industrial application.Compared with traditional Pd / C catalyst, the catalyst has higher activity and stability.

The application belongs to the field of pharmaceutical products, and particularly relates to a squalene modified polyphenol-chlorophyll flexible nanoliposome as well as a preparation method and application thereof. An organic phase containing phospholipid substances, squalene and the like is prepared, a lipid film is obtained through rotary evaporation and vacuum drying, and the lipid film is hydrated with a polyphenol buffer, ultrasonicated and extruded through a filter membrane to obtain the nanoliposome. The nanoliposome promotes scarless wound healing through three mechanisms of antioxidation, antibiosis and anti-fibrosis, has skin affinity and super-flexible penetration properties, and has excellent stability and transdermal properties, and can be used for preparing wound repair and drug delivery drugs, and has a wide application prospect.

This application discloses a squalene / calcium phosphotungsten oleogel, its preparation method, and its applications, relating to the field of biomedical technology. The squalene / calcium phosphotungsten oleogel is composed of squalene and calcium phosphotungsten nanowires; by mass percentage, the calcium phosphotungsten nanowire content is 2-5%, and the squalene content is 95-98%. The squalene / calcium phosphotungsten oleogel exerts anti-inflammatory and antioxidant effects by scavenging excess ROS, and can restore cell membrane fluidity, weaken mechanosensitive YAP pathway activation, and synergistically promote photoaging repair and scarless healing through multiple pathways. This comprehensive regulatory strategy provides a new solution for skin damage repair.

This invention discloses an unsaponifiable extract of olive oil, its preparation method, and its applications. The preparation method includes: S1, raw material pretreatment: after drying and pulverizing olive pulp, antioxidants and a compound enzyme are added for enzymatic hydrolysis, followed by ultrasonic cell disruption; S2, solvent extraction: extraction is performed using a hexane-anhydrous ethanol compound solvent to obtain an extract; S3, multi-stage saponification: the extract undergoes two stages of saponification sequentially, and the organic phase is collected; S4, purification and refining: after dehydration and concentration, the organic phase is dissolved in squalane and filtered to obtain a refined concentrate. This invention achieves efficient and gentle extraction of high-value components such as squalene and phytosterols through low-temperature processing, enzyme-ultrasonic synergistic cell disruption, and gradient saponification. The obtained product can be directly used in cosmetics, exhibiting excellent anti-aging and skin repair effects, and the formulation is extremely simple, gentle, and non-irritating, showing promising application prospects.

The application provides a squalene fermentation liquor and a method for determining the content of extracted process sample acetone, n-heptane, ethanol and squalene, relates to the field of chemical industry, and specifically comprises the following steps: S1, preparing a test solution: determination of acetone, n-heptane and ethanol: accurately weighing n-propanol and a sample, and shaking to obtain; determination of squalene: accurately weighing squalene and n-propanol in a volumetric flask, adding ethyl acetate to the scale, and shaking to obtain; the test solution is detected by using a gas chromatography method, and the content of acetone, n-heptane, ethanol and squalene is calculated by using an internal standard method; the linear relationship between the theoretical concentration and the detected concentration in the concentration range is investigated, ethanol r=0.999, the average recovery rate is 101%; acetone r=1.000, the average recovery rate is 99%; n-heptane r=1.000, the average recovery rate is 100%; and squalene r=1.000, the average recovery rate is 100%.

This invention discloses the application of *Pteris vittata* oil in skincare products. The preparation method of the *Pteris vittata* oil involves first extracting the raw material using supercritical CO2 extraction technology to obtain crude *Pteris vittata* oil; then purifying the crude *Pteris vittata* oil through multi-stage molecular distillation, collecting the light fraction to obtain the final *Pteris vittata* oil. This preparation method can efficiently enrich the antioxidant active ingredients such as tocopherols, squalene, and polyunsaturated fatty acids in the *Pteris vittata* oil. The prepared *Pteris vittata* oil exhibits excellent scavenging ability against DPPH free radicals, superoxide anions, and ABTS free radicals, while significantly enhancing SOD activity, reducing MDA levels, and minimizing oxidative stress damage, thereby exerting antioxidant and anti-aging effects. This provides a safe, gentle, and highly effective natural raw material for anti-aging, whitening, and skin barrier repair skincare products, and has promising market application prospects.

The present invention relates to a process for obtaining minor components from vegetable oils, and wherein the vegetable oil includes palm oil, palm olein, palm mid-fraction or mixtures thereof. The minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, a fractionation in a plate short path evaporator. The fractionation is conducted in a plate short path evaporator with at least one evaporation section at a temperature of 140°C to 180°C, followed by at least one evaporation section at temperature of above 180°C to 240°C. The feed stream may be a condensate stream from a deodorization step.

This invention relates to the field of vaccine adjuvant technology, and discloses an oil-in-water adjuvant containing squalene and α-tocopherol, its preparation method, and its application. The preparation method includes the following steps: mixing squalene, α-tocopherol, and poloxamer to obtain an oil phase mixture; subjecting the oil phase mixture to high-speed shearing with an aqueous phase to obtain a pre-emulsion; and subjecting the pre-emulsion to high-pressure homogenization to obtain the oil-in-water adjuvant. This invention, through the synergistic effect of α-tocopherol and poloxamer, combined with a specific preparation process, solves the problems of poor stability and uneven particle size distribution in existing adjuvants. The adjuvant prepared by this invention exhibits excellent long-term physicochemical stability, with a Z-average particle size in the range of 100-300 nm and a polydispersity index (PDI) of less than 0.25, demonstrating good immunostimulant carrying capacity and immune enhancement effect.

This invention belongs to the field of bioengineering technology and provides the application of P450 enzyme in catalyzing the oxidation of squalene. Several cytochrome P450 genes were cloned from the full-length transcriptome data of the medicinal plant *Alisma plantago-aquatica*. Preliminary screening of the gene sequences corresponding to the P450 enzyme was conducted using bioinformatics. Recombinant vectors were prepared using the screened genes and transformed into squalene-producing yeast for gene function verification. This demonstrated the oxidation effect of the P450 enzyme encoded by the AoCYP_3836 gene on squalene, providing a novel reaction mode different from the traditional 2,3-epoxidation reaction of squalene. The results show that the P450 enzyme encoded by the AoCYP_3836 gene can catalyze the oxidation of squalene to produce two new products: squalene-1,1'-diol and squalene-1-carboxylic acid-24-ol, providing a new pathway for the production of these squalene oxides.

This invention discloses an engineered methanogenic bacterium that produces high levels of squalene, its construction method, and its applications, belonging to the fields of metabolic engineering and synthetic biology. This is achieved by using a strong promoter in the host bacterium (methanogenic bacterium). P mxaF Expression of squalene synthase encoding gene sqs Overexpression of the gene encoding 1-deoxy-xylulose-5-phosphate synthase in the squalene synthesis pathway dxs1 and dxs2 Farnesyl pyrophosphate synthase encoding gene ispA Isopentenyl pyrophosphate isomerase encoding gene My friends Simultaneously overexpressing glucose-6-phosphate dehydrogenase zwf1 and the EMP pathway pfk1 and fba2 An engineered methanogenic bacterium producing squalene was obtained. Using this engineered methanogenic bacterium for squalene fermentation production can significantly increase squalene yield and has excellent prospects for industrial application.

This invention discloses a method for preparing anthocyanin-coated nanoparticles and their applications. First, anthocyanins and extracellular vesicles (EVs) are extracted from honeysuckle fruit to obtain anthocyanin powder. Then, PBS and squalene are added to the anthocyanin powder to obtain squalene@anthocyanin. Next, EVs are added to squalene@anthocyanin, co-incubated, and then centrifuged to obtain anthocyanin-coated nanoparticles. These nanoparticles have a particle size of 180-220 nm, a zeta potential of -45 to -55 mV, and an encapsulation efficiency ≥85%. They exhibit excellent stability and biocompatibility, possessing functions such as regulating gut microbiota, antidepressant, antioxidant, and anti-inflammatory effects. Furthermore, their good biocompatibility and low toxicity solve the problems of stability and bioavailability, achieving precise regulation of gut microbiota and providing a safe and efficient new approach for the treatment of depression.

This invention discloses a ternary supramolecular eutectic solvent, its preparation method, and its applications. The ternary supramolecular eutectic solvent is composed of a hydrogen bond acceptor, a hydrogen bond donor, and a silver salt. In application, the solvent is mixed with vegetable oil, and through assisted enhanced extraction, phase separation, and back-extraction, high-purity squalene can be obtained efficiently. This invention utilizes the synergistic effect of the hydrophobic inclusion of cyclodextrin and the specific complexation of double bonds by silver ions, significantly enhancing the targeted binding and separation ability of squalene. This solvent system is green and low in toxicity, simple to prepare, and the extraction method is highly efficient and selective. The solvent can be recycled, overcoming the shortcomings of existing technologies such as low extraction efficiency, high pollution, and high cost. It has broad application prospects in the field of green extraction of plant-derived squalene.

The application belongs to the technical field of diesel anti-wear agents, and specifically provides a composite diesel anti-wear agent and a preparation method thereof, and a preparation method of the composite diesel anti-wear agent, which is obtained by mixing and treating fatty acid ester, base oil, Fischer-Tropsch wax, squalene and tocopherol acetate; the preparation steps comprise the following: S01. mixing the base oil and the Fischer-Tropsch wax, heating to complete dissolution of the Fischer-Tropsch wax under a nitrogen atmosphere to obtain a first mixture; S02. adding the fatty acid ester, the squalene and the tocopherol acetate to the first mixture, stirring and uniformly mixing to obtain a second mixture; S03. supplementing the base oil to the second mixture, continuing to stir and then cooling to room temperature to obtain the composite diesel anti-wear agent. The composite diesel anti-wear agent prepared in the application has good emulsion resistance and wear resistance.

The application discloses a squalene-based rubber antioxidant and a preparation method thereof. The preparation method comprises the following steps: S1, dissolving squalene in an organic solvent, then adding m-chloroperbenzoic acid, and obtaining an epoxidized squalene solution after reaction; S2, adding an inorganic alkali solution into the above solution, separating and removing an aqueous phase, and then removing a solvent in an organic phase to obtain epoxidized squalene; S3, adding the epoxidized squalene into dimethylbenzene to dissolve, adding p-aminodiphenylamine and an organic amine catalyst, and heating in an inert gas atmosphere, and obtaining a squalene-based antioxidant solution after reaction, and finally removing the solvent and the catalyst to obtain the squalene-based rubber antioxidant. The prepared antioxidant has good compatibility with common non-polar rubber, has a plasticizing effect, is beneficial to rubber processing, can participate in a rubber vulcanization reaction, effectively reduces migration and precipitation of the antioxidant in a rubber product during storage and use, and effectively improves anti-aging performance of the rubber product.

The application provides a saccharomyces cerevisiae engineering bacterium for producing squalene through endogenous and artificial synthetic pathways and an application thereof, the saccharomyces cerevisiae engineering bacterium takes ZS00 strain as a starting strain, overexpresses acetyl coenzyme A acetyltransferase gene, hydroxymethylglutaryl coenzyme A synthase gene and N-terminal truncated hydroxymethylglutaryl coenzyme A reductase gene, and effectively improves the yield of squalene. Heterologous expression of mevalonate kinase gene and mevalonate pyrophosphate decarboxylase gene, overexpression of the key gene squalene synthase gene for promoting the conversion of precursors into squalene effectively promotes the synthesis of squalene. The introduction of isopentenol utilization pathway genes and their mutants, through the regulation of IU pathway key substrate, overexpression of isopentenyl pyrophosphate isomerase gene and farnesyl pyrophosphate synthase gene, the final strain can effectively accumulate squalene to 687.93 mg / L. The application realizes the breakthrough of squalene yield and efficiency through complementation and synergistic effect.

This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.

Disclosed are: yeast which is capable of highly producing squalene, the yeast being genetically modified in such a manner that the expression or activity of at least one enzyme in the ergosterol biosynthesis pathway can be increased or decreased and being also genetically modified in such a manner that the expression of a protein involved in the synthesis of a cell wall can be increased or decreased; and a method for producing squalene, the method including a step for culturing the yeast in a culture medium.

The present invention provides a vaccine comprising a VZV gE protein and a complex adjuvant, wherein the VZV gE protein is amino acids 1-546 of wild-type gE, and the complex adjuvant comprises an emulsion of squalene and / or squalane and CpG. The present invention also provides methods of making the vaccine, and uses of the vaccine for preventing herpes zoster disease and its complications.

The application discloses a method for synthesizing natural products by using methanol yeast chassis cells and an engineering strain, relates to the technical field of genetic engineering, and comprises a squalene engineering strain.The construction of the squalene engineering strain comprises the following steps: cloning a rate-limiting enzyme gene in Pichia pastoris GS115 into a vector pPICZA with a PAOX1 promoter, electrically transforming a linearized recombinant plasmid into the Pichia pastoris GS115, integrating the linearized recombinant plasmid into a genomic HIS4 site, screening positive transformants, and obtaining a first strain.The first strain is synthesized, the squalene synthesis capacity is established in the chassis cells, the problem that the wild-type Pichia pastoris has a very low endogenous mevalonate pathway flow is solved, the rate-limiting bottleneck of the squalene synthesis pathway is eliminated, and the precursor supply level is improved.

The present invention relates to a process for obtaining minor components from vegetable oils, wherein the minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, an enzymatic condensation of a feed stream in presence of a lipase followed by a fractionation in a plate short path evaporator. The fractionation is conducted in a plate short path evaporator with at least one evaporation section at a temperature of from 140°C to 180°C, followed by at least one evaporation section at temperature of from 180°C to 240°C. The feed stream may be a condensate stream from a deodorization step.

The present disclosure provides, a vaccine composition that comprises an Epstein Barr Virus (EBV) polypeptide and a squalene nanoemulsion (SNE) adjuvant, and methods of inducing an immune response to an Epstein Barr Virus (EBV) or methods of preventing infection of or reducing the likelihood of infection by an Epstein Barr Virus (EBV) using the compositions, or a combination of the EBV polypeptide and the SNE adjuvant.

The application discloses a dammaradiene synthase and application thereof, and belongs to the technical field of bioengineering.The application aims to provide a protein with dammaradiene synthase and a key amino acid site capable of transforming a triterpene cyclase OSC protein into a catalytic active site for synthesizing dammaradiene.The application provides a dammaradiene synthase, and the amino acid sequence of the dammaradiene synthase is shown as SEQ ID NO.3.It is proved by a site-directed mutation experiment that the mutation of Y260N of the homologous gene of the Panax notoginseng dammaradiene synthase OeOSC14 can make the OSC catalyze the efficient synthesis of dammaradiene from squalene.

The present invention relates to a process for obtaining minor components from vegetable oils and wherein the vegetable oil includes palm oil, palm olein, palm mid-fraction or mixtures thereof. The minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, a fractionation in short path evaporator and a further fractionation in short path evaporator at a temperature of from 180 to 240°C. The feed stream may be a condensate stream from a deodorization step.

Squalene hopene cyclase (SHC) enzyme and its variants and their use in the preparation of (-)-ambromide from bis- ...