235 results about "Squalene" patented technology

The invention belongs to the technical field of biological medicine, and particularly discloses a composite vaccine adjuvant as well as a preparation method and application thereof. The composite vaccine adjuvant provided by the invention is an oil-in-water emulsion, and simultaneously contains squalene, polysorbate 80, sorbitan trioleate, polyinosinic acid (Poly I: C) and CpG oligodeoxynucleotide (CpG-ODN). The sequence-optimized CpG-ODN and Poly I: C are used in a specific emulsion system in a combined mode, a remarkable synergistic immune enhancement effect can be generated, the induced humoral immunity and cellular immunity response level is remarkably higher than the sum of the effects when all the components are independently used, and the composite adjuvant is good in stability, high in immunostimulatory activity and suitable for clinical application. The immune effect can be remarkably improved by combined application with various vaccine antigens. The invention also provides a preparation method and application of the composite vaccine adjuvant.

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The invention discloses an anti-oxidation system. The anti-oxidation system is prepared from 0.05 v / v% to 1 v / v% of squalene and 0.2 mM to 5 mM of Trolox. According to the anti-oxidation system provided by the invention, the squalene and the Trolox are combined for use, so that the anti-oxidation function of the Trolox is exerted to the greatest extent; and by using squalene, the problem of pH value fluctuation caused by using a Gloxy and glucose mixed solution can be solved, and the antioxidant system provided by the invention can realize an excellent oxygen removal effect under the condition of not influencing the pH value of the system.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention discloses an application of squalene or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing or treating endotoxemia. Experiments show that squalene significantly relieves LPS-induced endotoxemia liver injury through a multi-target mechanism, squalene not only relieves inflammatory response by inhibiting release of inflammatory factors, but also relieves oxidative stress by activating an antioxidant pathway, and the squalene has dual action mechanisms. The invention further provides application of the squalene-containing composition to preparation of the medicine for treating endotoxemia, squalene and glucocorticoid show a synergistic effect in the aspect of inhibiting TNF-alpha, AST can be reduced more remarkably by combining squalene and glucocorticoid, and the effect of dual inhibition of inflammatory mediators is achieved. Besides, the composition of the inducible nitric oxide synthase and squalene is applied to the preparation of the medicine for treating endotoxemia, and the inducible nitric oxide synthase inhibitor can inhibit the activity of iNOS, so that the excessive generation of nitric oxide is reduced, and the inflammatory reaction is further inhibited synergistically.

The invention discloses an emulsifier for preparing a Pickering emulsion, the Pickering emulsion as well as a preparation method and application of the Pickering emulsion. The emulsifier of the Pickering emulsion is obtained by dissolving distearoyl phosphatidylcholine, cholesterol and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 in ethanol, carrying out rotary evaporation to remove ethanol, then adding an antigen aqueous solution containing an antigen, continuing rotary evaporation to obtain liposome nanoparticles, heating the liposome nanoparticles with mannose in a water bath, and carrying out freeze drying. The Pickering emulsion is obtained by dispersing an emulsifier in water as a water phase, taking squalene as an oil phase, mixing the water phase and the oil phase, and homogenizing. The Pickering emulsion disclosed by the invention is prepared by taking antigen presenting cell targeted liposome nanoparticles as a raw material; and the antigen has excellent ion concentration characteristic, pH stability, temperature stability and storage stability, and can protect the integrity of the antigen.

The present invention discloses a preparation method of Ganoderma lucidum spore oil and Grifola frondosa soft capsules based on a cold preparation method. First, Grifola frondosa extract, selenium-enriched yeast, squalene, and Ganoderma lucidum spore oil are subjected to high-speed shear nano-processing and then set aside; subsequently, gelatin is subjected to low-temperature gelation with glycerol, water, and cocoa shell extract to obtain a gelatin solution, and finally, soft capsules are prepared. While ensuring that gelatin is fully dissolved, the present invention effectively avoids the impact of traditional gelation temperature on gel strength, viscosity, three-dimensional structure, production stability, and other properties and application effects. At the same time, the Ganoderma lucidum spore oil and squalene, Grifola frondosa extract, and selenium-enriched yeast are cleverly combined to significantly increase the content of triterpenoid compounds, a characteristic component in the contents, thereby enhancing the liver's metabolic detoxification ability and the body's anti-inflammatory and immunomodulatory effects.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention discloses melogabalin besylate tablets and a preparation method thereof, and relates to the technical field of medicines. The melogabalin besylate tablet is prepared from melogabalin besylate, a filling agent, a disintegrating agent, a stabilizing agent, a lubricating agent and an antioxidant composition, the antioxidant composition comprises tartaric acid and squalene. The preparation process is optimized, a uniform and stable solid dispersion is prepared by means of a hot melt extrusion process, and it is guaranteed that active raw materials and the antioxidant are uniformly mixed; the antioxidant composition with compatibility of tartaric acid and squalene is screened, and the stability of the antioxidant in the long-term storage process is remarkably improved.

The invention discloses a self-emulsifying adjuvant of a vaccine and a preparation method of the self-emulsifying adjuvant, and relates to the technical field of vaccine adjuvants. The adjuvant comprises an oil phase, an emulsifier and a freeze-drying protective agent, the oil phase comprises squalene and tocopherol, the emulsifier comprises polysorbate and a polyoxyethylene-polyoxypropylene block copolymer, and the freeze-drying protective agent comprises sugar and sugar alcohol. The preparation method comprises the following steps: emulsifying an oil phase and a water phase to form a nano-emulsion, and then freeze-drying to obtain freeze-dried powder. The adjuvant has the advantages of small particle size, uniform distribution, high stability, rapid reconstruction and the like, and can effectively maintain active components and improve the immune effect of vaccines.

The invention relates to the technical field of soothing compositions, and discloses a camellia oil composition for soothing dry, itching and red skin and a preparation method of the camellia oil composition. 0.2 to 5 parts of natural vitamin E; 20 to 70 parts of glycerol; 0.1 to 5 parts of a nonionic emulsifier; 1 to 10 parts of water; in the camellia oil, the content of triterpene alcohol is greater than or equal to 1500mg / kg; the content of squalene is greater than or equal to 100mg / kg; the nonionic emulsifier is prepared from polyglycerol-6 stearate and glyceryl stearate. Camellia oil rich in triterpenol and squalene is adopted as a raw material, and the soothing effect can be effectively improved; meanwhile, the natural vitamin E is added, so that the active ingredients of triterpene alcohol and squalene in the camellia oil can be stabilized; and the camellia oil and the natural vitamin E are prepared into an intermediate which can be directly applied to water aqua, cream and cleaning products, and meanwhile, the stability of the products is improved.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention relates to the technical field of synthetic biology, in particular to a squalene high-yield saccharomyces cerevisiae engineering bacterium and a construction method and application thereof.Saccharomyces cerevisiae CEN.PK.2-1D is used as an original strain, pentose phosphate pathway genes ZWF1, GND1, TAL1 and TKL1 and IDP1 genes of TCA circulation are integrated in a genome of the original strain through homologous recombination, and the squalene high-yield saccharomyces cerevisiae engineering bacterium is obtained. Constructing a dual-channel cofactor supply system to improve the intracellular NADPH level and the NADPH / NADP + ratio; according to the saccharomyces cerevisiae genetic engineering strain Sc-Sq04 constructed by the method disclosed by the invention, the yield of squalene obtained by shake flask fermentation reaches 741.08 + / -28.9 mg / L by further integrating all genes of an MVA way, ERG20 and ERG9 genes, and a new thought is provided for transforming saccharomyces cerevisiae to synthesize terpene compounds and promoting industrial application.

The invention relates to the technical field of nutritious food, and discloses a DHA microcapsule and a preparation method thereof. The DHA microcapsule contains a core material and a wall material, the core material contains DHA oil, squalene and carotenoid, the carotenoid is selected from at least one of beta-carotene, alpha-carotene, lycopene, xanthophyll and zeaxanthine, and the mass ratio of the DHA oil to the squalene to the carotenoid is 1: (0.1-0.55): (0.04-0.16). The DHA microcapsule has relatively low surface oil and a relatively good embedding effect, and active substances in the DHA microcapsule have a relatively high retention rate.

Disclosed herein are reagents, reagent kits, and culture media for activating and expanding immune cells in vitro. In some embodiments, the reagents, reagent kits, and culture media comprise a Quillaja saponin. In other embodiments, the reagents, reagent kits, and culture media comprise a squalene-based emulsion. Also disclosed are methods for activating and expanding immune cells in vitro using the reagents, reagent kits, and culture media.

SILICONE-FREE COSMETIC COMPOSITIONS This disclosure relates to compositions including squalene, at least one bio-based branched hydrocarbon, at least one bio-based pentaerythrityl ester, and processes for using such compositions. The cosmetic composition is silicone-free. The cosmetic composition spreads easily and has favorable sensory properties without the use of silicones. Figure for the abstract: none

The formula comprises the following raw materials in parts by weight: 70-85 parts of base liquor, 5-15 parts of fresh pandan leaves, 0.5-3 parts of baked pandan leaf powder, 1-3 parts of a flavor modifier, 3-8 parts of a sugar source and 0.1-0.6 part of a functional additive. The unique flavor of the pandan leaves originates from the rich 2-acetyl-1-pyrroline, and the pandan leaves give out charming rice dumpling fragrance and have the effects of promoting appetite and metabolism at the same time. The pandan leaves are blended into the food, so that the delicious taste is improved, and additional nutritional value is also brought. The pandan leaves are rich in dietary fibers, vitamin B family, vitamin C and various minerals. The composition also contains squalene, linoleic acid, estragole, phytol and other active ingredients. Squalene is a natural organic terpenoid, widely exists in a living body, and has remarkable effects in the aspects of promoting blood circulation, resisting oxidation, resisting inflammation, resisting tumors and the like. The prepared pandan wine has multiple effects and is beneficial to body health after being drunk for a long time.

The present invention relates to a process for obtaining minor components from vegetable oils, and wherein the vegetable oil includes palm oil, palm olein, palm mid-fraction or mixtures thereof and wherein the minor components of interest are squalene, tocopherols, and optionally tocotrienols. The process is comprising, a fractionation in short path evaporator followed by a further fractionation in short path evaporator. The feed stream may be a condensate stream from a deodorization step.

The present application relates to oxidized squalene cyclase, recombinant vector, recombinant engineering bacteria and application thereof, and preparation method of parkol. The oxidized squalene cyclase comprises: a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1; or a polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1 with one or more amino acids being deleted, substituted or added; or a polypeptide having at least 80% homology with the polypeptide consisting of the amino acid sequence shown as SEQ ID No. 1. The above-mentioned oxidized squalene cyclase can catalyze 2, 3 oxidized squalene to produce parkol, and the enzyme can be expressed according to the coding sequence of the cyclase, and then the substrate is catalyzed to improve the yield of parkol.

The invention relates to a squalene epoxidase mutant and application thereof. The amino acid sequence of the squalene epoxidase mutant is an amino acid sequence obtained by deleting amino acids from the first site to the 55th site at the N end of an amino acid sequence of wild squalene epoxidase SgSQE1 from momordica grosvenori, and then connecting a dissolution-promoting tag sequence to the N end and / or the C segment. The squalene epoxidase mutant provided by the invention can be used for remarkably improving the yield of 2, 3, 22, 23-diepoxy squalene.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V based on multi-compartment multi-metabolic pathways and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The preparation method comprises the following steps: anchoring an MVA pathway and a squalene synthesis pathway on the surface of a peroxisome membrane, and enabling mogrol synthetase and epoxy squalene cyclization enzyme ERG1 to form a linear polywheat complex through polypeptide interaction; then, through overexpression of MVA pathway key enzyme and introduction of an isopentenol utilization pathway, supply of precursor isopentenyl pyrophosphate and dimethyl allyl pyrophosphate in cytoplasm is increased, and squalene epoxidase ERG1 and mogrol synthetase are anchored in transmembrane ABC efflux protein PDR11 through polypeptide interaction; then anchoring the mogrol synthetase in the lipid droplet subcells; finally, the glucosyltransferase is displayed on the surface of the yeast cell wall through an alpha-lectin system, so that extracellular mogrol is converted into MG-V. The engineering bacterium improves the yield of mogroside V synthesized from the beginning in microorganisms, and has great industrial application value.

The present invention discloses a method for promoting the synthesis of 7-dehydrocholesterol by Saccharomyces cerevisiae. By mutating phosphomevalonate kinase ERG8 and squalene synthase ERG9 and integrating them into the Saccharomyces cerevisiae genome separately or simultaneously, several mutant strains with significantly increased 7-dehydrocholesterol production are obtained. Among them, phosphomevalonate kinase ERG8 involves mutations at one or more sites such as 75, 187, 192, 205, 206, 314, 322, 349, etc., and squalene synthase ERG9 involves mutations at one or more sites such as 96, 108, 146, 208, 209, 251, 252, 258, 286, 345, 355, 388, 389, 404, 426, 434, etc. The Saccharomyces cerevisiae mutants provided by the present invention can achieve the production of 7-DHC, laying a foundation for metabolic engineering transformation of Saccharomyces cerevisiae to promote 7-DHC synthesis.

The present disclosure relates to microbial cells, engineered enzymes, and methods for producing mogrol or mogrosides. The methods include providing a microbial cell that produces squalene and expresses a biosynthetic pathway that converts squalene to the mogrol or mogrosides, and culturing the microbial cell under conditions suitable for producing the mogrol or mogrosides. In various embodiments, the methods disclosed herein produce squalene derivatives in which the extrapathway products cucurbitadienols, hydrolyzed 2,3;22,23-dioxidosqualene, and glycosylated 24,25-dihydroxycucurbitadienols are less than about 40% by weight.

The present disclosure relates to compositions comprising squalene, at least one bio-based branched hydrocarbon, at least one bio-based pentaerythritol ester, and methods of using such compositions. The cosmetic composition is free of silicone. The cosmetic composition is easy to smear and has good sensory properties even without using silicone.

The invention discloses an immunogenic composition containing gE nanoparticles as well as a preparation method and application thereof, and relates to the technical field of biomedicine, the immunogenic composition comprises self-assembled gE nanoparticles and an oil-in-water emulsion adjuvant, and a self-assembled gE nanoparticle antigen comprises gE, a connecting peptide SGS and a gI polypeptide sequence containing Th epitopes; the oil-in-water type emulsion adjuvant is prepared from squalene, Tween 80, DL-alpha-tocopherol and / or Span 85. The immunogenic composition provided by the invention can be applied to vaccines for preventing varicella and / or herpes zoster and / or postherpetic neuralgia, so that the aims of improving the immunogenicity of the gE antigen and reducing side effects of the vaccines are fulfilled.

The invention provides a construction method of a multi-gene synergistic expression vector and application of the multi-gene synergistic expression vector in improvement of oil tea squalene synthesis, and belongs to the technical field of plant genetic engineering and metabolic engineering. MAPK, PP1 and HMGR gene segments are obtained through an RT-PCR technology, the MAPK, PP1 and HMGR gene segments are cloned to expression cassettes containing independent promoters and terminators respectively, and after Insulator isolation elements are inserted, the expression cassettes are integrated to a pCAMBIA1300 vector through a modular assembly technology. Wherein the HMGR gene contains S47A and T208A mutation sites so as to enhance the enzyme activity. The vector constructed by the invention can cooperatively express a target gene in plant cells, so that accurate regulation and control on the phosphorylation state of HMGR are realized. When the method is applied to tobacco or camellia oleifera callus, the squalene content is effectively increased, the method has the advantages of multi-target regulation and control, efficient and flexible construction, high universality and the like, and an efficient tool is provided for anabolism enhancement of natural products.

The invention provides camellia oil containing nano squalene and a preparation method thereof, and relates to the technical field of food processing, in particular to camellia oil containing nano squalene. According to the scheme, a high-pressure homogeneous emulsification technology and a lipid-surfactant synergistic stabilization mechanism are adopted; the preparation method comprises the following steps: firstly, beeswax (solid lipid), camellia oil and rice oil (liquid lipid) are fused at high temperature to form a uniform oil phase; the interfacial tension is reduced through high-speed stirring, in the high-pressure homogenizing process, lipid liquid drops are broken to nanoscale through mechanical shearing force and the hole effect, and soybean lecithin molecules rapidly wrap new liquid drops to prevent aggregation. And finally, quickly cooling to recrystallize and solidify the beewax, wherein a crystalline region of the beewax can enhance the rigidity of emulsion droplets, lock a nano structure and uniformly disperse squalene to obtain the nano functional camellia oil. Solid lipid of the nano-carrier can form a physical barrier, the contact rate of squalene and oxygen is reduced, the oxidation rate is reduced, and the preparation method is simple in process, high in operability and suitable for large-scale mass production.

The invention belongs to the technical field of biological medicine, and particularly relates to a small-molecule exosome-loaded composition based on bone marrow mesenchymal stem cells and application of the small-molecule exosome-loaded composition. The invention discloses the treatment effect of squalene on osteoarthritis for the first time, and further prepares the squalene-loaded bone marrow mesenchymal stem cell exosome. An in-vitro cell experiment and a model animal experiment prove that the composition has a synergistic treatment effect on osteoarthritis. In-vitro tests prove that the squalene-loaded mesenchymal stem cell exosome can improve the survival rate of inflammatory cartilage cells, inhibit release of proinflammatory factors and restore expression of repair factors. Model animal experiments prove that the squalene-loaded mesenchymal stem cell exosome has the effects of resisting inflammation, easing pain and protecting cartilage in the treatment of osteoarthritis.

This invention pertains to modern agriculture, specifically the application of transcription factors CdZF-HD1 / 9 in regulating squalene synthesis. Using the CdSQS promoter as bait, this invention screens a yeast single-hybrid library to identify ZF-HD transcription factors that interact with the CdSQS promoter. ZF‑HD1 and ZF‑HD9 .clone ZF‑HD1 and ZF‑HD9 The nucleotide sequence was further confirmed by dual-luciferase assay and EMSA. ZF‑HD1 and ZF‑HD9 Interacts with CdSQS. ZF‑HD1 and ZF‑HD9 Genetic transformation of Camellia oleifera from Vietnam resulted in increased expression levels of squalene synthase and higher squalene content in the induced hairy roots. This invention provides a scientific basis for finding optimal genetic modification and exogenous regulation methods to increase squalene content in Camellia oleifera, and offers important theoretical and practical support for promoting the development of the Camellia oleifera industry in Hainan.

The invention discloses a preparation method and application of nanoparticles based on anthocyanin coating, and the preparation method comprises the following steps: firstly, extracting anthocyanin and extracellular vesicles (EVs) from lonicera caerulea to obtain anthocyanin powder; then adding PBS (Phosphate Buffer Solution) and squalene into the obtained anthocyanin powder to obtain squalene-anthocyanin; and adding the EVs into squalene and anthocyanin, co-incubating, and carrying out centrifugal treatment, so as to obtain the anthocyanin-coated nanoparticles. The nanoparticle has the characteristics that the particle size is 180-220 nm, the Zeta potential is-45 mV to-55 mV, and the encapsulation efficiency is greater than or equal to 85%, has excellent stability and biocompatibility, has the functions of regulating intestinal flora, resisting depression, resisting oxidation, resisting inflammation and the like, is good in biocompatibility and low in toxicity, solves the problems of stability and bioavailability of the nanoparticle, realizes accurate regulation and control of the intestinal flora, and has a good application prospect. And a safe and efficient new way is provided for treating depression.