208 results about "Triterpene" patented technology

Provided is the use of a traditional Chinese medicine composition in preparation of a drug for preventing major adverse cardiac and cerebrovascular events after percutaneous coronary intervention and ameliorating myocardial injury and vascular endothelial dysfunction after percutaneous coronary intervention. The traditional Chinese medicine composition is composed of total salvianolic acid and total hawthorn triterpene acid.

The invention relates to a triterpenoid saponin compound, a preparation method thereof and application of the triterpenoid saponin compound in preparation of anti-neuroinflammation drugs. The triterpenoid saponin compound has a structure as shown in a formula I. An in-vitro anti-neuroinflammation activity experiment proves that the triterpenoid saponin compound has an obvious inhibition effect on lipopolysaccharide (LPS)-induced BV2 cell inflammation, and can be used as a novel anti-neuroinflammation drug and a neurodegenerative disease treatment drug. Formula I

The application discloses a compound with the function of purgation and water excretion, which has a chemical structural formula as shown in formula (I). The compound is a new natural product of tetracyclic triterpenes, has good fat solubility and chemical stability, can be conveniently extracted from the rhizome of the herbal medicine Alisma orientale and prepared into an oral or injection dosage form. The application further provides a preparation method and application of the compound. The compound can obviously inhibit the increase of the abdominal volume and the weight of ascites of mice in a dose-dependent manner, and is a new water excretion drug with great potential.

The application discloses a norchizhiol triterpenoid compound, a preparation method and application thereof. The norchizhiol triterpenoid compound norchizhiol A can be quickly prepared from Ganoderma lucidum. The test proves that the norchizhiol A can inhibit the accumulation of triglyceride in the differentiation process of 3T3L1 preadipocytes. The norchizhiol A can also effectively reduce the weight increase and liver coefficient of high-fat-diet-induced obese mice, regulate the blood lipid level of the obese mice, and significantly intervene and improve the lipoprotein distribution of the body. The compound can be used for preparing anti-obesity and lipid-lowering medicines.

The present application relates to the technical field of biological medicine, and discloses a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia, after the ganoderma lucidum thick piece is treated by the processes of spraying moistening, hot pressure steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpene and sterol is improved, and the release rate of active ingredients is also significantly improved. The structure of the ganoderma lucidum mycelium cell wall is damaged by the present application technology, so that the active ingredients such as polysaccharide, triterpene and sterol originally existing in the ganoderma lucidum can be easily extracted and released. The ganoderma lucidum cells are loosened and broken in the present application, and the polysaccharide in the combined state can be changed into the free state, so that the content of the ganoderma lucidum polysaccharide can be greatly improved. The active ingredients in the ganoderma lucidum can be quickly and maximally released in the digestive tract by directly taking the broken wall superfine powder of the broken cells, so that the effect of the superfine powder on improving female insomnia is improved.

The application provides a varicella-zoster virus vaccine and application thereof, and belongs to the technical field of vaccines.The varicella-zoster virus vaccine comprises liposome nanoparticles, zoster virus glycoprotein E and an adjuvant encapsulated in the liposome nanoparticles.The adjuvant comprises triterpenoid saponins.In the application, the zoster virus glycoprotein E (gE) is wrapped by the liposome nanoparticles, which can effectively promote antigen-presenting cells to phagocytose and efficiently deliver the antigen, and realize sustained stimulation of the vaccine to the body to produce a specific cellular immune response against VZV-gE.The triterpenoid saponins used in the application can effectively realize cross-presentation of the antigen zoster virus glycoprotein E and induce an antigen-specific cellular immune response.In addition, the cholesterol rich in the liposome nanoparticles can effectively neutralize the cytotoxicity of the triterpenoid saponins, ensuring the safety of the vaccine.

This invention discloses a class of pentacyclic triterpenoid derivatives with diverse B-ring modifications, their preparation methods, and applications. This invention utilizes a chemical enzymatic method to expand the chemical space of the B-ring in pentacyclic triterpenoids, obtaining a series of pentacyclic triterpenoid derivatives with diverse B-ring modifications. Pharmacological activity screening of these pentacyclic triterpenoid derivatives revealed their good tumor-suppressive activity, providing a promising application prospect for the prevention and treatment of tumors. This invention achieves the efficient synthesis of pentacyclic triterpenoid derivatives with diverse B-ring modifications, structures that are difficult to obtain using traditional chemical methods. Systematic antiproliferative activity screening identified candidate molecules with broad-spectrum and highly efficient inhibitory effects against various human tumor cell lines. These molecules show good drug development potential, providing novel lead compounds for the innovative development of anticancer drugs.

The invention belongs to the field of fermentation engineering, and particularly discloses a preparation method and application of edible fungus and probiotic co-culture fermentation liquor. A bidirectionally promoted edible fungus-probiotic symbiotic metabolism system is constructed by adopting a synchronous inoculation technology and combining with an optimized culture medium formula taking grain processing materials such as coarse rice powder as a main substrate. According to the method, in the culture period of 7-14 days, the fermentation liquor with the total triterpene content not lower than 35 mg / g, the total polysaccharide content not lower than 25 mg / g and the viable count of probiotics reaching 1 * 10 < 8 > CFU / mL or above can be obtained. The technology has the advantages of being easy and convenient to operate, low in cost and short in period, the content of the active ingredients of the edible fungi is remarkably increased while the viable count of the probiotics is maintained, and a new technical scheme is provided for developing high-quality functional food or preparations with the probiotic function and the active ingredients of the fungi.

The utility model discloses supercritical extraction equipment for high-purity ganoderma triterpenes, which relates to the technical field of extraction equipment and comprises a supporting outer frame, a supercritical extraction equipment body is arranged at one end of the inner side of the supporting outer frame, a driving cylinder is fixed at the center of the upper end of the supporting outer frame, and the telescopic end of the driving cylinder penetrates through the supporting outer frame and is fixedly provided with a guide rail seat. A connecting plate is fixed at one end, close to the supercritical extraction equipment body, of the guide rail seat; the screw is sleeved with a carrying seat, a driving assembly is arranged at one end of the carrying seat and located on the outer side of the screw, and an outer receiving seat is arranged at the other end of the carrying seat and located on the outer side of the screw. According to the supercritical extraction equipment for the high-purity ganoderma triterpenes, ground ganoderma raw materials are conveyed to the inner side of the inner receiving seat, the outer receiving seat is driven to move to the inner side of the reaction kettle cavity, then the overturning assembly is used for achieving overturning control over the outer receiving seat and the inner receiving seat, and automatic feeding is achieved.

The invention relates to the technical field of medicines, in particular to a novel triterpenoid glycoside compound extracted from rosa roxburghii tratt fruits and a preparation method and application of the novel triterpenoid glycoside compound. The novel triterpenoid glycoside compound is Roxbuterene E, the molecular formula of the novel triterpenoid glycoside compound is C41H66O13, the novel triterpenoid glycoside compound is obtained by separating and purifying a rosa roxburghii tratt fruit methanol extract by adopting normal-phase silica gel column chromatography, reversed-phase ODS column chromatography and HPLC (High Performance Liquid Chromatography), and a PTP1B enzyme inhibitory activity experiment proves that the novel triterpenoid glycoside compound has potential activity for treating diabetes. And technical support is provided for research and development of novel diabetes treatment drugs.

This application relates to the technical field of traditional Chinese medicine compositions, specifically disclosing a traditional Chinese medicine composition for treating deficiency syndromes caused by illness, surgery, radiotherapy, and chemotherapy, and its preparation process. The composition comprises Ganoderma lucidum extract and ginseng powder. The preparation method of the Ganoderma lucidum extract is as follows: Step 1, pulverizing the raw material to obtain Ganoderma lucidum powder; Step 2, extraction: extracting the Ganoderma lucidum powder with a primary liquid-liquid phase solvent for 1.5-2.5 hours, adding non-oxidizing gas nano-bubble water and extracting for 0.5-1.5 hours, then adding acidic gas nano-bubble water and continuing extraction for 0.5-1.5 hours; Step 3, collection: collecting the extract, concentrating, forming a paste, and drying to obtain the Ganoderma lucidum extract; the primary liquid-liquid phase solvent includes an alcohol solution with a volume ratio of (2-5):1 and a non-polar organic solvent, with the alcohol volume percentage in the primary alcohol solution being 65-90%. The composition of this application has a higher content of Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes.

The application provides a key enzyme involved in glycosylation modification of triterpene saponins, and an application thereof, and belongs to the technical field of genetic engineering. The glycosyltransferase gene is screened based on the omics research of the base plant of traditional Chinese medicine Duanxueliu, i.e. clinopodium gracile, and a pET28a-MBP-CcGT-g51295 prokaryotic expression vector is constructed to successfully obtain the glycosyltransferase. It is found that the glycosyltransferase can catalyze Saikogenin A and Saikogenin F to generate Prosaikogenin A and Prosaikogenin F respectively by using a glycosyl donor UDP-fucose; can catalyze Saikogenin A to generate clinopodiside I and clinopodiside X by using UDP-glucose; can catalyze Prosaikogenin A to generate Saikosaponin B1, Saikosaponin c1 and Saikosaponin X by using UDP-glucose; and can catalyze Prosaikogenin F to generate Saikosaponin A and Buddlejasaponin IV by using UDP-glucose, which reveals the catalytic function of the glycosyltransferase and provides a gene component for the biological research of triterpene saponins, and solves the problem of biological resources of rare saponins.

The invention provides lucid ganoderma tea as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine tea. The preparation method of the lucid ganoderma tea comprises the following steps: crushing lucid ganoderma, adding water to infiltrate, steaming at high pressure and drying; the mass ratio of the lucid ganoderma powder to the water is 1: (1-4) when the water is added for infiltration, and the time is 20-200 min; during the high-pressure steaming, the temperature is 100 to 115 DEG C, the pressure is 0.030 to 0.090 Mpa, and the time is 30 to 90 minutes; and further packaging to prepare ganoderma lucidum tea bags. In the ganoderma lucidum tea prepared by the invention, the content of ganoderma lucidum polysaccharide is more than or equal to 9.0 mg / g, and the content of triterpenes and sterols is more than or equal to 5.0 mg / g, so that the ganoderma lucidum tea has the functions of strengthening the spleen and stomach and calming the heart and tranquilizing the mind, can be used for preparing medicines or health care products for treating or adjunctively treating tumors, insomnia, amnesia, poor appetite and body deficiency, and can also be used for adjunctively treating patients with increased glutamic-pyruvic transaminase.

The invention relates to a mycelium extracted from ganoderma lucidum and a preparation method of a culture medium of the mycelium, belongs to the technical field of mycelium extraction, and aims to solve the problems that in the prior art, ganoderma lucidum mycelia grow slowly and are prone to pollution, the content of effective components is unstable, and molecular identification is difficult. The invention provides the solid culture medium prepared from the saussurea involucrata purple yellow extract, the extract is formed by combining effective components of saussurea involucrata and sodium alginate, the growth rate and metabolism efficiency of ganoderma lucidum hyphae can be improved, active substances such as polysaccharides and triterpenes are effectively enriched, and the yield of the ganoderma lucidum hyphae is increased. Meanwhile, the stress resistance and the survival rate of the mycelium under the stress conditions of oxidative stress, pathogen invasion and the like are enhanced, the pollution risk in the culture process is reduced, the biosynthesis efficiency and the extracellular secretion level of key secondary metabolites are improved, the immunocompetence and the bioavailability of the product are further improved, and the production cost is reduced. And a stable and reliable technical support is provided for large-scale and high-quality production of ganoderma lucidum.

The present application relates to triterpene glycoside saponin-derived adjuvants, syntheses thereof, and intermediates thereto. The application also provides pharmaceutical compositions comprising compounds of the present invention and methods of using said compounds or compositions in the treatment of and immunization for infectious diseases.

The invention provides a triterpene enantiomer compound as well as a preparation method and application thereof, and relates to the technical field of medicines. The preparation method of the triterpenoid enantiomer compound comprises the following steps: (1) preparing a lycopodium clavatum extract: crushing a dried lycopodium clavatum whole plant, adding the crushed lycopodium clavatum whole plant into an ethanol solution for extraction, collecting an extracting solution, and concentrating the extracting solution under reduced pressure into paste to obtain a lycopodium clavatum crude extract; and (2) separation and purification: adding the crude lycopodium clavatum extract in the step (1) into deionized water for dilution, preparing a suspension, sequentially extracting with petroleum ether and chloroform, concentrating the chloroform extract into extract, and performing column chromatography, thin layer chromatography and molecular sieve chromatography separation to obtain the target triterpenoid enantiomer compounds named (+)-oncerin A (1) and (-)-oncerin A (2). The invention provides a new path for developing a new acetylcholin esterase inhibitor drug, and the application prospect is good.

The invention discloses a fermentation method for fungus-enzyme synergistic treatment of lucid ganoderma and application of the method in pet food. The fermentation method comprises the following steps: (1) enzymolysis pretreatment: mixing ganoderma lucidum sporocarp powder with water, adding cellulase and laccase, and performing enzymolysis to obtain an enzymolysis solution; (2) sterilizing and supplementing a carbon source: adding the carbon source into the enzymatic hydrolysate, and sterilizing; (3) lactobacillus fermentation: adding lactobacillus plantarum into the sterilized enzymatic hydrolysate, and fermenting under a fermentation condition to obtain fermentation liquor; and (4) solid-liquid separation: performing centrifugal separation on the fermentation liquor, and collecting supernate to obtain the ganoderma lucidum fermentation liquor. According to the method, the laccase and the cellulase are synergistically broken, so that the release efficiency of triterpenes and total phenols in the ganoderma lucidum with spores is remarkably improved. The fermentation liquor can improve lipid accumulation (the oil red O staining lipid content is reduced) and relieve oxidative stress injury on the cell level, and can be used for preparing functional food for improving metabolic health, especially for lipid abnormality and oxidative stress state related to metabolic dysfunction of pets.

The application discloses a compound nanomicelle co-loading icariin and glycyrrhiza triterpene saponin and a preparation method thereof. The compound nanomicelle comprises icariin, glycyrrhiza triterpene saponin and an amphiphilic block copolymer carrier material, and through a self-assembly process in an aqueous medium, the compound nanomicelle is formed, in which icariin and glycyrrhiza triterpene saponin are simultaneously and stably wrapped by the block copolymer carrier material. The prepared compound nanomicelle has uniform particle size, high encapsulation rate and good stability, can effectively overcome the medicine-making defects of icariin and glycyrrhiza triterpene saponin, and obtain a synergistic pharmacological effect of combination.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The present application provides vaccines comprising an inactivated influenza virus and an adjuvant comprising a triterpenoid saponin, a sterol, and an immunostimulatory oligonucleotide comprising CpG. Also provided are methods of using such vaccines.

The invention relates to the field of daily chemicals, and particularly discloses a plant extract with a skin repairing effect as well as a preparation method and application of the plant extract. The plant extract with the skin repairing effect is prepared from the following raw materials in parts by mass: 1-4 parts of lycium barbarum roots and 3-5 parts of pomelo fruits, and the weight ratio of the lycium barbarum roots to the pomelo fruits is (1-4): (3-5). The preparation method comprises the following steps: preparing the boxthorn root extract, and preparing and preparing the pomelo fruit extract. The preparation method comprises the following steps: adding an alcohol solvent into the lycium barbarum root extract and the pomelo fruit extract, uniformly mixing and stirring, carrying out micron-sized filtration, and carrying out heat preservation at 90-95 DEG C; the obtained plant extract is characterized by comprising more than or equal to 1.8% of total flavonoids and more than or equal to 0.8% of total triterpenes. The product is mild and safe, good in formula compatibility and stable in compatibility, has a broad-spectrum light protection effect of completely covering the ultraviolet band of 290-400 nm, can reduce damage to the skin caused by solarization, and has a good sunburn and sunburn prevention effect.

The invention relates to the field of medicinal chemistry and medicine, and discloses pentacyclic triterpene derivatives and application thereof in prevention and / or treatment of immune diseases. The pentacyclic triterpene derivative disclosed by the invention has a structure as shown in a formula (I) and has an excellent immunoregulation effect on Tc1, Tc17 and Th17 cells, and the invention also provides application of the compound in preparation of medicines for preventing and / or treating immune diseases.

Provided are a Gynostemma pentaphyllum-derived triterpenoid saponin compound and a use thereof in the preparation of anti-fatigue products. The provided triterpenoid saponin compound is selected from one of compounds 1-29. Also provided are a use of a triterpenoid saponin compound as represented by general formula I in the preparation of anti-fatigue products. Experiments show that the Gynostemma pentaphyllum-derived triterpenoid saponin compound has significant anti-fatigue activity and can be used for preparing anti-fatigue products.

The invention provides a lipidosome adjuvant system based on furanose type QS-21 saponin molecules and a preparation method. The lipidosome adjuvant system comprises lipidosome and the QS-21 saponin molecules, the lipidosome comprises a lipid bilayer formed by ionizable lipid, structural phospholipid and cholesterol, the QS-21 saponin molecule is in a furanose type and is loaded on the lipidosome, and the QS-21 saponin molecule is partially inserted into the lipid bilayer through hydrophobic triterpenoid aglycone of the QS-21 saponin molecule. The preparation method comprises the following steps: dissolving lipidosome components forming lipid bilayers in an organic solvent to form a lipid phase, dissolving furanose type QS-21 saponin molecules in an acidic aqueous phase buffer solution to form a QS-21 aqueous phase, mixing the lipid phase and the QS-21 aqueous phase, precipitating lipid, and self-assembling to form lipidosome nanoparticles loaded with furanose type QS-21 saponin molecules, and finally, purifying to remove the organic solvent and the unencapsulated QS-21 molecules. According to the invention, through well-designed lipid composition and a preparation process, furanose type QS21 molecules are stably integrated into the lipidosome.

An induction medium and induction culture method for lavender suspension cells having high antioxidant activity. Suspension cells having a high ABTS free radical scavenging rate can be cultured by optimizing the formulation of a medium, and the contents of total phenols, total flavonoids and total triterpenoids are relatively high. Additionally, lavender suspension cells induced by using the medium grow rapidly and have a stable system, and extracts thereof can significantly reduce redundant ROS generated by oxidative stress of HaCaT cells, exhibit good biological antioxidant activity, and provide a reference for the development of lavender secondary metabolite resources and the like.

This invention discloses an antitumor natural drug composition based on Norway maple and its preparation method, relating to the field of biomedical technology. The compound is selected from at least one of the following structures: 3-O-[β-D-glucopyranosyl-(1→4)-[β-D-galactopyranosyl-(1→2)]]-β-D-glucopyranoside-21-O-acetyl-22-O-angeloyl yrutile alcohol, with the molecular formula C2. 55 H 86 O 23 This invention isolates a class of triterpenoid saponins with a rutinol skeleton from the bark of Norwegian maple, enriching the chemical composition library of maple species. The compounds contain unique oligosaccharide chains and diacyl substitution patterns at C-21 and C-22. One compound has a rare branched acyl group (3-hydroxy-4-methylhexanoyl) at C-21, while the other compound contains an arabinofuranose unit in its C-3 trisaccharide chain. These structural features are rare in natural products and demonstrate excellent structural novelty.

The invention discloses a pentacyclic triterpene acylhydrazone compound as well as a preparation method and application thereof. A series of oleanolic acid derivatives are synthesized by carrying out structural modification on carboxyl at C28 site of oleanolic acid, structural confirmation is carried out by using 1H NMR, 13C NMR, HRMS and X-ray single crystal diffraction, and the inhibition effect of the pentacyclic triterpenoid acylhydrazone compound on the activity of tumor cells A549, AGS and K562 is evaluated by taking cis-platinum as positive control and adopting a CCK-8 method. Results show that the inhibition effect of the derivatives 5, 6, 9, 10, 16, 21, 27 and 28 on tumor cells is superior to that of cis-platinum. Reference is provided for research of oleanolic acid derivatives and discovery of anti-tumor seedling compounds.

To provide a composition for promoting tight junction formation that promotes formation of tight junctions between epithelial tissue cells.SOLUTION: A composition for promoting tight junction formation for promoting formation of tight junctions between epithelial tissue cells, contains triterpenes and skim milk and / or wheat gluten, thereby solving the problem to be solved.SELECTED DRAWING: None

The invention relates to a phellinus igniarius polysaccharide extraction method, which comprises: adopting phellinus igniarius sporocarp, adding a composite extraction solvent, carrying out synchronous enzymatic hydrolysis and extraction with a composite enzyme, carrying out ultrasonic treatment, collecting a filtrate I obtained by filtering the ultrasonic extraction solution, carrying out vacuum concentration on the filtrate I, adding an ethanol solution, mixing, filtering again, and collecting a filter cake and a filtrate II after filtering to obtain the phellinus igniarius polysaccharide. And drying the filter cake under reduced pressure to obtain the phellinus igniarius polysaccharide. The composite extraction solvent is a mixed solution of an ethanol solution, propylene glycol and water according to the volume ratio of (0.4-0.5): (0.1-0.2): (0.3-0.4). The extraction efficiency of phellinus igniarius polysaccharide and phellinus igniarius triterpene active ingredients is effectively improved, the activity and long-term storage stability of the phellinus igniarius polysaccharide are improved, and after the active ingredients are further processed and prepared into the flash release freeze-dried buccal tablets, the disintegration efficiency and the dissolution rate of the buccal tablets are improved, so that the bioavailability of the buccal tablets is improved.

The present application relates to the field of biological medical technology, in particular to a traditional Chinese medicine composition as GSK-3beta inhibitor and preparation and application thereof. The traditional Chinese medicine composition as GSK-3beta inhibitor comprises gastrodin and ganoderma triterpene, and the mass ratio of gastrodin and ganoderma triterpene is (1-4):1. The traditional Chinese medicine composition provided by the present application can inhibit the overexpression of GSK-3beta in AD model, thereby inhibiting the overphosphorylation of Tau protein and playing a protective role on Alzheimer's disease. In addition, the traditional Chinese medicine composition provided by the present application contains gastrodin and ganoderma triterpene, which are homoeopathic traditional Chinese medicine efficacy components, greatly improving the safety of the drug, and providing a train of thought for the application of the traditional Chinese medicine composition as a functional food.