367 results about "Triterpene" patented technology

The invention discloses a ganoderma lucidum fermentation liquid capable of improving the content of total triterpenes and immunity and a preparation method and application thereof, the preparation method comprises the following steps: heating a ganoderma lucidum liquid, and carrying out hot water extraction to obtain a ganoderma lucidum extracting liquid; heating the lucid ganoderma extracting solution to be boiled, after sterilization is finished, after the lucid ganoderma liquid is cooled, adding lactic acid bacteria powder in a specific proportion for fermentation to obtain lucid ganoderma fermentation liquid; and carrying out solid-liquid separation on the fermentation liquor to obtain fermentation supernate, and sterilizing the ganoderma lucidum fermentation liquor to obtain the sterilized ganoderma lucidum fermentation liquor. According to the preparation method disclosed by the invention, the content of total triterpenes in the ganoderma lucidum fermentation liquor is increased, the immunomodulatory activity of the ganoderma lucidum product can be enhanced, and the ganoderma lucidum product has relatively high effects of resisting oxidation, improving the activity of phagocytes, improving the activity of acid phosphatase, improving the immunity and the like, and has a wide application prospect and market potential.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to the technical field of pharmaceutical preparations, in particular to a multiple nano emulsion adjuvant and a preparation method thereof. The multiple nano emulsion adjuvant is prepared from 2.73 to 6.37 g of an oil phase matrix based on 100 g of a raw material system, 0.10 to 0.25 g of a sterol compound; 0.212 to 0.424 g of a nonionic surfactant; 0.00050 to 0.00200 g of a triterpenoid saponin type active adjuvant; 0.010 to 0.020 g of an anionic polymer modifier; 0.005 to 0.015 g of a cationic polymer modifier; 70-90 g of a buffer solution; the total mass of the raw materials is 100 g. The nano emulsion adjuvant prepared by the invention can be stored for 6 months at 2-8 DEG C under a low sterol condition, the particle size change does not exceed + 5%, no crystallization or layering is generated, and the stability of the active adjuvant is remarkably improved.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention belongs to the technical field of medicine, and discloses a sea cucumber and ganoderma lucidum immune steady-state compound and a composite enzymatic hydrolysis targeted liposome co-delivery preparation process thereof.The sea cucumber and ganoderma lucidum immune steady-state compound is composed of sea cucumber, ganoderma lucidum, American ginseng, dried oyster meat, fructus lycii and perilla leaves; the ganoderma triterpenes release free polysaccharides and are combined with a liposome intestinal targeted delivery system, so that the absorption rate of the polysaccharides is greatly improved to 18-22% from 5% in the traditional process, the ganoderma triterpenes are emulsified and entrapped through a liposome double-layer membrane, and meanwhile, the permeation promotion effect of perillaldehyde in the perilla leaf volatile oil is supplemented, so that the transmembrane transport efficiency is remarkably enhanced, the absorption rate is improved to 12-15% from 2%, and the bioavailability is improved. The zinc element decomposes oyster zinc protein into free zinc ions through a composite enzymolysis technology, and the free zinc ions and vitamin C form a stable chelate, so that the antagonism of intestinal tracts is reduced, the zinc absorption rate is increased from 20% to 45-50%, and a solid foundation is laid for fully playing a compound immune regulation effect.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The invention discloses an engineered glycosyl transferase and application thereof in efficient preparation of triterpenoid saponin. The engineered glycosyl transferase is obtained by mutating serine at the 15th site of UGT74AC1 enzyme into alanine, mutating histidine at the 47th site into aspartic acid, mutating leucine at the 48th site into valine, mutating alanine at the 180th site into serine, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid The mutant is obtained by mutating serine at the 332 site into proline, phenylalanine at the 367 site into tryptophan and lysine at the 420 site into arginine. According to the invention, the glycosyl transferase with high catalytic activity is obtained by modifying an enzyme engineering technology, and good application of the glycosyl transferase in synthesis of triterpenoid saponin is realized. The glycosyl transferase disclosed by the invention can also be coupled with sucrose synthase, or coupled with N-acetylhexosamine kinase and N-acetylglucosamine-1-uridine phosphate transferase, and acetylglucosamine is used as a glycosyl donor, so that cascade production of triterpenoid saponin with lower cost and higher efficiency is realized.

The present invention discloses a preparation method of Ganoderma lucidum spore oil and Grifola frondosa soft capsules based on a cold preparation method. First, Grifola frondosa extract, selenium-enriched yeast, squalene, and Ganoderma lucidum spore oil are subjected to high-speed shear nano-processing and then set aside; subsequently, gelatin is subjected to low-temperature gelation with glycerol, water, and cocoa shell extract to obtain a gelatin solution, and finally, soft capsules are prepared. While ensuring that gelatin is fully dissolved, the present invention effectively avoids the impact of traditional gelation temperature on gel strength, viscosity, three-dimensional structure, production stability, and other properties and application effects. At the same time, the Ganoderma lucidum spore oil and squalene, Grifola frondosa extract, and selenium-enriched yeast are cleverly combined to significantly increase the content of triterpenoid compounds, a characteristic component in the contents, thereby enhancing the liver's metabolic detoxification ability and the body's anti-inflammatory and immunomodulatory effects.

The invention relates to edible and medicinal fungi, in particular to a new strain of ganoderma leucocontextum and application thereof. The preservation number of the ganoderma leuconium RK001 (Ganoderma leuconium RK001) is CCTCC (China Center for Type Culture Collection) NO: M20232555, and the preservation number of the ganoderma leuconium RK001 is CCTCC NO: M20232555. The new strain Ganoderma leucoderma RK001 has the advantages of high content of crude polysaccharides and total triterpenes, short growth cycle and high yield, is suitable for artificial cultivation, and has high popularization value.

The invention relates to the technical field of concrete materials, in particular to a formula of plateau high-cold high-speed railway bridge pier column anti-crack concrete which comprises the following components: 280-320 kg / m < 3 > of Portland cement, 80-100 kg / m < 3 > of mineral powder, 60-80 kg / m < 3 > of coal ash, 800-850 kg / m < 3 > of machine-made sand with the fineness of 2.6-3.0, 900-1000 kg / m < 3 > of broken stone, 140-160 kg / m < 3 > of water and 4.0-5.0 kg / m < 3 > of polycarboxylic acid water reducing agent. The total use amount of the retarding components in the water reducing agent is 0.8-1.2 kg / m < 3 >, the air entraining agent comprises triterpenoid saponin and a liquid air entraining agent, the total use amount is 0.02-0.05 kg / m < 3 >, and the use amount of the polypropylene fiber is 0.9-1.2 kg / m < 3 >. Through a low water-binder ratio, a composite gelling system, fiber reinforcement and seasonal regulation and control composite admixture technology, the compressive strength, crack resistance and freeze-thaw resistance are remarkably improved, and the composite admixture is suitable for bridge engineering in plateau alpine regions.

The invention relates to the technical field of soothing compositions, and discloses a camellia oil composition for soothing dry, itching and red skin and a preparation method of the camellia oil composition. 0.2 to 5 parts of natural vitamin E; 20 to 70 parts of glycerol; 0.1 to 5 parts of a nonionic emulsifier; 1 to 10 parts of water; in the camellia oil, the content of triterpene alcohol is greater than or equal to 1500mg / kg; the content of squalene is greater than or equal to 100mg / kg; the nonionic emulsifier is prepared from polyglycerol-6 stearate and glyceryl stearate. Camellia oil rich in triterpenol and squalene is adopted as a raw material, and the soothing effect can be effectively improved; meanwhile, the natural vitamin E is added, so that the active ingredients of triterpene alcohol and squalene in the camellia oil can be stabilized; and the camellia oil and the natural vitamin E are prepared into an intermediate which can be directly applied to water aqua, cream and cleaning products, and meanwhile, the stability of the products is improved.

The present invention discloses a fermentation method for Antrodia camphorata mycelium and an auxiliary composition for fermentation of Antrodia camphorata. The method provided by the present invention comprises the following steps: S1. subjecting an Antrodia camphorata strain to liquid fermentation for 60-120 hours; S2. adding precursors to the fermented Antrodia camphorata strain liquid fermentation broth, and continuing the fermentation for more than 120 hours to obtain Antrodia camphorata mycelium; the precursors being coenzyme Q0, p-hydroxybenzoic acid, linalool, and α-terpineol, and the fermentation broth after adding the precursors having a coenzyme Q0 content of 100-500 mg / L, a p-hydroxybenzoic acid content of 20-300 mg / L, a linalool content of 0.1-0.5 mg / L, and an α-terpineol content of 0.3-0.7 mg / L. This method adds precursor substances of specific composition and concentration to the liquid fermentation broth of Antrodia cinnamomea at a certain time and combines them with each other, which is beneficial to the secondary metabolism of Antrodia cinnamomea and promotes the synthesis of triterpenoid compounds, thereby increasing the yield. Compared with the existing technology, the yield of triterpenoid compounds produced by Antrodia cinnamomea mycelium is increased, the culture cycle is shortened, and it is more suitable for large-scale production.

The present invention provides triterpenoid compounds in Ganoderma sinense fermented mycelia. The structural formulas of the triterpenoid compounds are Structural Formulas 1, 3, 4, and 7. The present invention also provides the application of the triterpenoid compounds in Ganoderma sinense fermented mycelia as a drug for preventing or treating inflammation; the triterpenoid compounds in the Ganoderma sinense mycelia are one or more of the compounds with Structural Formulas 1-8. The present invention for the first time conducts an anti-inflammatory activity test on the triterpenoid compounds isolated and purified from Ganoderma sinense mycelia, and finds that the compounds with Structural Formulas 1-8 can all inhibit the release of TNF-α and IL-6, and have significant anti-inflammatory activity.

Provided is the use of a traditional Chinese medicine composition in preparation of a drug for preventing major adverse cardiac and cerebrovascular events after percutaneous coronary intervention and ameliorating myocardial injury and vascular endothelial dysfunction after percutaneous coronary intervention. The traditional Chinese medicine composition is composed of total salvianolic acid and total hawthorn triterpene acid.

The invention relates to the field of lucid ganoderma cultivation, and particularly discloses a method for increasing the content of ganoderma triterpenes in lucid ganoderma, which comprises the following steps: S1, preparing a culture medium; step S2, basswood manufacturing; step S3, inoculation; step S4, mycelium culture; step S5, burying soil and growing ganoderma lucidum; step S6, forming; from two aspects, firstly, a fermentation accelerant is added during preparation of the culture medium, and the fermentation accelerant is prepared by mixing calcium lactate, salicylic acid, a citrate-phosphate buffer solution and hydroxypropyl-beta-cyclodextrin according to a specific mass ratio; secondly, in the soil burying and ganoderma lucidum growing process, culture medium soil rich in fermentation extract is adopted for culture, the fermentation extract is prepared into a fermentation substrate through compound bacteria fermentation by taking Chinese wolfberry residues, liquorice residues and astragalus membranaceus residues as raw materials, and the content of ganoderma lucidum triterpenes in ganoderma lucidum is increased through the synergistic effect of all the substances.

The invention provides an extraction method of cynanchum thesioides fruit total triterpenes and the extracted cynanchum thesioides fruit total triterpenes, and belongs to the technical field of plant extraction. By controlling the relational expression of process parameters in the ultrasonic extraction process, the extraction rate of the cynanchum thesioides fruit total triterpenes can be regulated and controlled by controlling the relationship among the material-liquid ratio, the ultrasonic time and the ultrasonic temperature, and the maximum value of the extraction rate of the cynanchum thesioides fruit total triterpenes is obtained according to the relational expression. Therefore, the extraction method provided by the invention can be used for optimizing the process conditions for extracting the cynanchum thesioides fruit total triterpenes. According to the method, the cynanchum thesioides fruit total triterpenes are extracted in an ultrasonic-assisted manner, the extraction process is simple, expensive equipment does not need to be introduced, the cost is low, and the method is suitable for industrial large-scale popularization. The result of the embodiment shows that the extraction rate of the cynanchum thesioides fruit total triterpenes obtained by the extraction method provided by the invention can reach 6.69 mg / g.

The invention relates to a triterpenoid saponin compound, a preparation method thereof and application of the triterpenoid saponin compound in preparation of anti-neuroinflammation drugs. The triterpenoid saponin compound has a structure as shown in a formula I. An in-vitro anti-neuroinflammation activity experiment proves that the triterpenoid saponin compound has an obvious inhibition effect on lipopolysaccharide (LPS)-induced BV2 cell inflammation, and can be used as a novel anti-neuroinflammation drug and a neurodegenerative disease treatment drug. Formula I

The application discloses a compound with the function of purgation and water excretion, which has a chemical structural formula as shown in formula (I). The compound is a new natural product of tetracyclic triterpenes, has good fat solubility and chemical stability, can be conveniently extracted from the rhizome of the herbal medicine Alisma orientale and prepared into an oral or injection dosage form. The application further provides a preparation method and application of the compound. The compound can obviously inhibit the increase of the abdominal volume and the weight of ascites of mice in a dose-dependent manner, and is a new water excretion drug with great potential.

The invention discloses a key enzyme farnesyl pyrophosphate synthase of a black fungus terpenoid synthetic route, and belongs to the technical field of protein. The invention aims to provide a key enzyme gene of a mevalonic acid pathway synthesis pathway in black fungus. The invention provides an FPPS protein of black fungus. The amino acid sequence of the FPPS protein is as shown in SEQ ID NO. 2. A solid foundation is laid for deeply exploring functions of key genes for triterpene synthesis, and meanwhile important data support is provided for systematically explaining specific effects of the genes in terpene synthesis routes.

The application discloses a norchizhiol triterpenoid compound, a preparation method and application thereof. The norchizhiol triterpenoid compound norchizhiol A can be quickly prepared from Ganoderma lucidum. The test proves that the norchizhiol A can inhibit the accumulation of triglyceride in the differentiation process of 3T3L1 preadipocytes. The norchizhiol A can also effectively reduce the weight increase and liver coefficient of high-fat-diet-induced obese mice, regulate the blood lipid level of the obese mice, and significantly intervene and improve the lipoprotein distribution of the body. The compound can be used for preparing anti-obesity and lipid-lowering medicines.

The present invention belongs to the field of plant genetic engineering technology, and in particular to the application of a Centella asiatica glycosyltransferase gene in catalyzing the glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is as shown in SEQ ID No.1, and the protein encoded by it can catalyze the C-28 position of asiatic acid and madecassic acid to carry out glucose group modification to produce asiatic acid monoglucoside and madecassic acid monoglucoside, and prefers catalysis madecassic acid. The present invention, by building a metabolic regulatory network, widely and accurately screens the above-mentioned gene, resolves the glycosylation process of catalyzing asiatic acid and madecassic acid, for the in vitro synthesis of Centella asiatica triterpenoid saponins and industrial production process to improve asiatic acid and madecassic acid glycosylation efficiency and asiaticoside and madecassin yield provide important reference basis, and provide important theoretical support for large-scale production of Centella asiatica active saponins.

The invention discloses a Ganoderma sinense flash-soluble tablet and a preparation method thereof, and belongs to the technical field of food health care, Ganoderma sinense sporocarp is subjected to deproteinization treatment to prevent precipitation generated by combination of tannic acid and Ganoderma sinense protein, and polysaccharide and triterpenes in Ganoderma sinense sporocarp are effectively retained and enriched after Ganoderma sinense sporocarp superfine powder is subjected to deproteinization treatment. In the preparation process, tannic acid serves as a structure trigger and is self-assembled with ganoderma sinense polysaccharide in deproteinized ganoderma sinense powder through hydrogen bonds to form a pH responsive hydration film, phenolic hydroxyl groups of tannic acid and alcoholic hydroxyl groups of beta-glucan form a multiple hydrogen bond network, aromatic rings are embedded into hydrophobic cavities of beta-glucan, and the compound is stabilized through the hydrophobic effect. By adopting hydroxypropyl-beta-cyclodextrin inclusion, the tannic acid is prevented from being combined with the ganoderma sinense sporocarp protein to form a precipitate in space. The Ganoderma sinensis flash-soluble tablet prepared by the invention is rich in triterpenes and polysaccharide components, can significantly improve immunity and regulate intestinal flora, and is suitable for health food development.

The present application relates to the technical field of biological medicine, and discloses a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia, after the ganoderma lucidum thick piece is treated by the processes of spraying moistening, hot pressure steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpene and sterol is improved, and the release rate of active ingredients is also significantly improved. The structure of the ganoderma lucidum mycelium cell wall is damaged by the present application technology, so that the active ingredients such as polysaccharide, triterpene and sterol originally existing in the ganoderma lucidum can be easily extracted and released. The ganoderma lucidum cells are loosened and broken in the present application, and the polysaccharide in the combined state can be changed into the free state, so that the content of the ganoderma lucidum polysaccharide can be greatly improved. The active ingredients in the ganoderma lucidum can be quickly and maximally released in the digestive tract by directly taking the broken wall superfine powder of the broken cells, so that the effect of the superfine powder on improving female insomnia is improved.

The invention belongs to the technical field of chemical medicines, and particularly relates to a novel triterpene alcohol ferulate compound as well as a preparation method and medical application thereof. According to the invention, oryzanol is used as a raw material, and the new triterpene alcohol ferulate compound is obtained through reversed-phase preparative high performance liquid chromatography and SFC chiral separation and purification. A method and a tool of network pharmacology are used for researching the action mechanism of potential anti-inflammatory and anti-infection related treatment of the new compound. Hierarchical analysis from a global network to a core target reveals a possible action mechanism of the compound in anti-inflammatory and anti-infection, and highlights a regulation mode of a key acting protein. Wherein IGF1R, CTSS, MMP2, BCL2 and other proteins may be core regulatory factors of compound mediated host immune regulation and bacterial drug resistance inhibition, and an important theoretical basis is provided for further research on molecular mechanisms and potential therapeutic targets of compounds in anti-inflammatory and anti-infection related treatment.

The application provides a varicella-zoster virus vaccine and application thereof, and belongs to the technical field of vaccines.The varicella-zoster virus vaccine comprises liposome nanoparticles, zoster virus glycoprotein E and an adjuvant encapsulated in the liposome nanoparticles.The adjuvant comprises triterpenoid saponins.In the application, the zoster virus glycoprotein E (gE) is wrapped by the liposome nanoparticles, which can effectively promote antigen-presenting cells to phagocytose and efficiently deliver the antigen, and realize sustained stimulation of the vaccine to the body to produce a specific cellular immune response against VZV-gE.The triterpenoid saponins used in the application can effectively realize cross-presentation of the antigen zoster virus glycoprotein E and induce an antigen-specific cellular immune response.In addition, the cholesterol rich in the liposome nanoparticles can effectively neutralize the cytotoxicity of the triterpenoid saponins, ensuring the safety of the vaccine.

This invention discloses a class of pentacyclic triterpenoid derivatives with diverse B-ring modifications, their preparation methods, and applications. This invention utilizes a chemical enzymatic method to expand the chemical space of the B-ring in pentacyclic triterpenoids, obtaining a series of pentacyclic triterpenoid derivatives with diverse B-ring modifications. Pharmacological activity screening of these pentacyclic triterpenoid derivatives revealed their good tumor-suppressive activity, providing a promising application prospect for the prevention and treatment of tumors. This invention achieves the efficient synthesis of pentacyclic triterpenoid derivatives with diverse B-ring modifications, structures that are difficult to obtain using traditional chemical methods. Systematic antiproliferative activity screening identified candidate molecules with broad-spectrum and highly efficient inhibitory effects against various human tumor cell lines. These molecules show good drug development potential, providing novel lead compounds for the innovative development of anticancer drugs.

The invention belongs to the field of fermentation engineering, and particularly discloses a preparation method and application of edible fungus and probiotic co-culture fermentation liquor. A bidirectionally promoted edible fungus-probiotic symbiotic metabolism system is constructed by adopting a synchronous inoculation technology and combining with an optimized culture medium formula taking grain processing materials such as coarse rice powder as a main substrate. According to the method, in the culture period of 7-14 days, the fermentation liquor with the total triterpene content not lower than 35 mg / g, the total polysaccharide content not lower than 25 mg / g and the viable count of probiotics reaching 1 * 10 < 8 > CFU / mL or above can be obtained. The technology has the advantages of being easy and convenient to operate, low in cost and short in period, the content of the active ingredients of the edible fungi is remarkably increased while the viable count of the probiotics is maintained, and a new technical scheme is provided for developing high-quality functional food or preparations with the probiotic function and the active ingredients of the fungi.