454 results about "Triterpene" patented technology

The invention discloses a ganoderma lucidum fermentation liquid capable of improving the content of total triterpenes and immunity and a preparation method and application thereof, the preparation method comprises the following steps: heating a ganoderma lucidum liquid, and carrying out hot water extraction to obtain a ganoderma lucidum extracting liquid; heating the lucid ganoderma extracting solution to be boiled, after sterilization is finished, after the lucid ganoderma liquid is cooled, adding lactic acid bacteria powder in a specific proportion for fermentation to obtain lucid ganoderma fermentation liquid; and carrying out solid-liquid separation on the fermentation liquor to obtain fermentation supernate, and sterilizing the ganoderma lucidum fermentation liquor to obtain the sterilized ganoderma lucidum fermentation liquor. According to the preparation method disclosed by the invention, the content of total triterpenes in the ganoderma lucidum fermentation liquor is increased, the immunomodulatory activity of the ganoderma lucidum product can be enhanced, and the ganoderma lucidum product has relatively high effects of resisting oxidation, improving the activity of phagocytes, improving the activity of acid phosphatase, improving the immunity and the like, and has a wide application prospect and market potential.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a centella asiatica glycosyl transferase gene in catalyzing glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the protein coded by the gene can catalyze the C-28 site of asiatic acid and madecassic acid to perform glucose group modification so as to generate asiatic acid monoglucoside and madecassic acid monoglucoside, and preferentially catalyzes madecassic acid. By constructing a metabolic regulation network, the genes are widely and accurately screened, and the glycosylation process of catalyzing asiatic acid and madecassic acid is analyzed; an important reference basis is provided for improving the glycosylation efficiency of asiatic acid and madecassic acid and the yield of asiaticoside and madecassoside in the in-vitro synthesis and industrial production process of the centella asiatica triterpenoid saponin, and an important theoretical support is provided for large-scale production of the centella asiatica active saponin.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to the technical field of pharmaceutical preparations, in particular to a multiple nano emulsion adjuvant and a preparation method thereof. The multiple nano emulsion adjuvant is prepared from 2.73 to 6.37 g of an oil phase matrix based on 100 g of a raw material system, 0.10 to 0.25 g of a sterol compound; 0.212 to 0.424 g of a nonionic surfactant; 0.00050 to 0.00200 g of a triterpenoid saponin type active adjuvant; 0.010 to 0.020 g of an anionic polymer modifier; 0.005 to 0.015 g of a cationic polymer modifier; 70-90 g of a buffer solution; the total mass of the raw materials is 100 g. The nano emulsion adjuvant prepared by the invention can be stored for 6 months at 2-8 DEG C under a low sterol condition, the particle size change does not exceed + 5%, no crystallization or layering is generated, and the stability of the active adjuvant is remarkably improved.

The invention provides an application of a pentacyclic triterpenoid compound targeting hydroxyl steroid 17-beta dehydrogenase 4. The pentacyclic triterpenoid compound can effectively relieve or treat central nervous system diseases (inflammatory diseases), including central nervous system degenerative diseases or chronic central inflammatory diseases. The pentacyclic triterpenoid compound can maintain the structural stability or activity of hydroxysteroid 17-beta dehydrogenase and maintain the stability or activity of peroxisome, thereby relieving or treating central nervous inflammation. The pentacyclic triterpenoid compound can target hydroxysteroid 17-beta dehydrogenase 4, inhibit neuroglial cell excessive activation related neuroinflammation, relieve neuron damage or death speed and improve a central nervous system cell metabolism microenvironment.

The invention belongs to the technical field of food microorganisms, and particularly discloses a ganoderma lucidum mutant strain capable of producing polysaccharides and triterpenoids at high yield. The strain is named as Ganoderma lucidum in Latin, the preservation number is CGMCC NO: 41680, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is December 4, 2024. The strain is named as Ganoderma lucidum, the preservation number is CGMCC NO: 41680, and the preservation number is CGMCC NO: 41680. The polysaccharide content in the ganoderma lucidum spore powder produced by the ganoderma lucidum mutant strain is more than or equal to 8%, and the total triterpenoid content is more than or equal to 12%; a culture medium formula of the ganoderma lucidum mutant strain comprises potato leachate, glucose and monopotassium phosphate, the culture temperature is 25-28 DEG C, and the pH value is 5.5-6.5. The ganoderma lucidum mutant strain is high in genetic stability, the content of active ingredients is remarkably higher than that of parent strains, and the ganoderma lucidum mutant strain is suitable for large-scale fermentation production.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The invention discloses a triterpenoid saponin compound derived from gynostemma pentaphylla and application of the triterpenoid saponin compound in preparation of an anti-fatigue product. The triterpenoid saponin compound provided by the invention is selected from one of the following compounds 1 to 29. The invention further provides application of the triterpenoid saponin compound shown in the general formula I in preparation of anti-fatigue products. Experiments show that the triterpenoid saponin compound derived from the gynostemma pentaphylla has remarkable anti-fatigue activity and can be used for preparing anti-fatigue products.

The invention belongs to the technical field of medicine, and discloses a sea cucumber and ganoderma lucidum immune steady-state compound and a composite enzymatic hydrolysis targeted liposome co-delivery preparation process thereof.The sea cucumber and ganoderma lucidum immune steady-state compound is composed of sea cucumber, ganoderma lucidum, American ginseng, dried oyster meat, fructus lycii and perilla leaves; the ganoderma triterpenes release free polysaccharides and are combined with a liposome intestinal targeted delivery system, so that the absorption rate of the polysaccharides is greatly improved to 18-22% from 5% in the traditional process, the ganoderma triterpenes are emulsified and entrapped through a liposome double-layer membrane, and meanwhile, the permeation promotion effect of perillaldehyde in the perilla leaf volatile oil is supplemented, so that the transmembrane transport efficiency is remarkably enhanced, the absorption rate is improved to 12-15% from 2%, and the bioavailability is improved. The zinc element decomposes oyster zinc protein into free zinc ions through a composite enzymolysis technology, and the free zinc ions and vitamin C form a stable chelate, so that the antagonism of intestinal tracts is reduced, the zinc absorption rate is increased from 20% to 45-50%, and a solid foundation is laid for fully playing a compound immune regulation effect.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention discloses a preparation method of a ganoderma spore oil grifola frondosa soft capsule based on a cold preparation method. The preparation method comprises the following steps: firstly, carrying out high-speed shearing and nanocrystallization treatment on grifola frondosa extract, selenium-enriched yeast, squalene and ganoderma lucidum spore oil for later use; and then melting gelatin, glycerol, water and cocoa shell extract at low temperature to obtain a gelatin solution, and finally preparing the soft capsule. According to the invention, on the basis of ensuring sufficient dissolution of the gelatin, the influence of the traditional gelatin melting temperature on the gel strength, the viscosity, the three-dimensional structure, the production stability and other performances and the application effect can be effectively avoided. Meanwhile, the ganoderma lucidum spore oil, squalene, grifola frondosa extract and selenium-enriched yeast are ingeniously combined, so that the content of a landmark component triterpenoid compound in the content is remarkably increased, and the metabolic detoxification capability of the liver and the anti-inflammatory and immune regulation effects of the body are enhanced.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The invention discloses a pharmaceutical composition for treating or improving Parkinson's disease and application of the pharmaceutical composition. The triterpenoid saponin compounds and the nervonic acid in different proportions are combined, and experiments prove that multiple indexes of Parkinson mice can be remarkably improved when the triterpenoid saponin compounds and the nervonic acid are combined in a specific proportion, and the effect of synergistically improving the Parkinson disease or the progress of the Parkinson disease is achieved. Therefore, the pharmaceutical composition disclosed by the invention can be used for more effectively treating or improving the Parkinson's disease, and has a wide application prospect in the field of treatment of the Parkinson's disease.

The invention discloses an engineered glycosyl transferase and application thereof in efficient preparation of triterpenoid saponin. The engineered glycosyl transferase is obtained by mutating serine at the 15th site of UGT74AC1 enzyme into alanine, mutating histidine at the 47th site into aspartic acid, mutating leucine at the 48th site into valine, mutating alanine at the 180th site into serine, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid The mutant is obtained by mutating serine at the 332 site into proline, phenylalanine at the 367 site into tryptophan and lysine at the 420 site into arginine. According to the invention, the glycosyl transferase with high catalytic activity is obtained by modifying an enzyme engineering technology, and good application of the glycosyl transferase in synthesis of triterpenoid saponin is realized. The glycosyl transferase disclosed by the invention can also be coupled with sucrose synthase, or coupled with N-acetylhexosamine kinase and N-acetylglucosamine-1-uridine phosphate transferase, and acetylglucosamine is used as a glycosyl donor, so that cascade production of triterpenoid saponin with lower cost and higher efficiency is realized.

The invention relates to the technical field of preparation methods of nutritional products, in particular to a preparation method of ganoderma lucidum spore oil with a liver regulating effect. Comprising the steps of preparing ganoderma lucidum spore powder, extracting ganoderma lucidum spore oil and enriching the ganoderma lucidum spore oil. Ganoderma lucidum blocks are treated through an ultrasonic extraction technology, ganoderma lucidum spore powder is prepared through a spray drying method, n-hexyl alcohol is used for soaking extraction, high-purity ganoderma lucidum spore oil is obtained through vacuum concentration and molecular distillation, and residual solvent is removed through film evaporation. Active ingredients such as triterpenoids and unsaturated fatty acid are further enriched by using supercritical CO2 extraction; the extraction efficiency and the purity of active ingredients of the ganoderma lucidum spore oil are remarkably improved, the obtained ganoderma lucidum spore oil has remarkable effects in the aspects of reducing liver toxicity indexes (ALT and AST) and enhancing antioxidant enzyme activity (SOD and GSH-Px), the operation controllability is high, the ganoderma lucidum spore oil is suitable for large-scale industrial application, and technical support is provided for research and development of liver protection drugs and functional food.

The present invention discloses a preparation method of Ganoderma lucidum spore oil and Grifola frondosa soft capsules based on a cold preparation method. First, Grifola frondosa extract, selenium-enriched yeast, squalene, and Ganoderma lucidum spore oil are subjected to high-speed shear nano-processing and then set aside; subsequently, gelatin is subjected to low-temperature gelation with glycerol, water, and cocoa shell extract to obtain a gelatin solution, and finally, soft capsules are prepared. While ensuring that gelatin is fully dissolved, the present invention effectively avoids the impact of traditional gelation temperature on gel strength, viscosity, three-dimensional structure, production stability, and other properties and application effects. At the same time, the Ganoderma lucidum spore oil and squalene, Grifola frondosa extract, and selenium-enriched yeast are cleverly combined to significantly increase the content of triterpenoid compounds, a characteristic component in the contents, thereby enhancing the liver's metabolic detoxification ability and the body's anti-inflammatory and immunomodulatory effects.

The invention relates to edible and medicinal fungi, in particular to a new strain of ganoderma leucocontextum and application thereof. The preservation number of the ganoderma leuconium RK001 (Ganoderma leuconium RK001) is CCTCC (China Center for Type Culture Collection) NO: M20232555, and the preservation number of the ganoderma leuconium RK001 is CCTCC NO: M20232555. The new strain Ganoderma leucoderma RK001 has the advantages of high content of crude polysaccharides and total triterpenes, short growth cycle and high yield, is suitable for artificial cultivation, and has high popularization value.

The invention relates to the technical field of biological medicines, and discloses a preparation method of ganoderma lucidum wall-broken superfine powder for female insomnia, which is characterized in that thick ganoderma lucidum slices are subjected to spray moistening, hot-pressing steam heating, ultrasonic treatment, microwave drying and other process treatments, so that the contents of polysaccharides, triterpenes and sterols are increased; and the release rate of the active ingredients is also obviously improved. According to the technology, the structure of the cell wall of the ganoderma lucidum mycelium is destroyed, so that active ingredients such as polysaccharide, triterpenes and sterol originally existing in ganoderma lucidum can be easily extracted and released. Ganoderma lucidum cells are loosened and broken, polysaccharide in a combined state can be changed into a free state, and the content of ganoderma lucidum polysaccharide can be greatly increased. The cell-broken ganoderma lucidum wall-broken superfine powder can be directly taken in digestive tracts to quickly release active ingredients in ganoderma lucidum to the maximum extent, and the effect of improving female insomnia by the superfine powder is improved.

The invention relates to the technical field of concrete materials, in particular to a formula of plateau high-cold high-speed railway bridge pier column anti-crack concrete which comprises the following components: 280-320 kg / m < 3 > of Portland cement, 80-100 kg / m < 3 > of mineral powder, 60-80 kg / m < 3 > of coal ash, 800-850 kg / m < 3 > of machine-made sand with the fineness of 2.6-3.0, 900-1000 kg / m < 3 > of broken stone, 140-160 kg / m < 3 > of water and 4.0-5.0 kg / m < 3 > of polycarboxylic acid water reducing agent. The total use amount of the retarding components in the water reducing agent is 0.8-1.2 kg / m < 3 >, the air entraining agent comprises triterpenoid saponin and a liquid air entraining agent, the total use amount is 0.02-0.05 kg / m < 3 >, and the use amount of the polypropylene fiber is 0.9-1.2 kg / m < 3 >. Through a low water-binder ratio, a composite gelling system, fiber reinforcement and seasonal regulation and control composite admixture technology, the compressive strength, crack resistance and freeze-thaw resistance are remarkably improved, and the composite admixture is suitable for bridge engineering in plateau alpine regions.

The invention relates to the technical field of soothing compositions, and discloses a camellia oil composition for soothing dry, itching and red skin and a preparation method of the camellia oil composition. 0.2 to 5 parts of natural vitamin E; 20 to 70 parts of glycerol; 0.1 to 5 parts of a nonionic emulsifier; 1 to 10 parts of water; in the camellia oil, the content of triterpene alcohol is greater than or equal to 1500mg / kg; the content of squalene is greater than or equal to 100mg / kg; the nonionic emulsifier is prepared from polyglycerol-6 stearate and glyceryl stearate. Camellia oil rich in triterpenol and squalene is adopted as a raw material, and the soothing effect can be effectively improved; meanwhile, the natural vitamin E is added, so that the active ingredients of triterpene alcohol and squalene in the camellia oil can be stabilized; and the camellia oil and the natural vitamin E are prepared into an intermediate which can be directly applied to water aqua, cream and cleaning products, and meanwhile, the stability of the products is improved.

The invention discloses a method for extracting effective components in poria cocos, which comprises the following steps: taking poria cocos raw powder, adjusting the moisture content of the poria cocos raw powder, then extruding the poria cocos raw powder, keeping the temperature at 30-90 DEG C in the extrusion process, and collecting poria cocos powder after the extrusion is completed; the collected poria cocos powder is crushed, dried and dissolved in a solvent for extraction. According to the method for extracting the effective components in the poria cocos, a traditional method for directly extracting the effective components in the poria cocos by utilizing a solvent is abandoned, the effective components in the poria cocos are innovatively extracted after treatment by adopting a low-temperature extrusion mode before the solvent is added, and cell walls of poria cocos powder are damaged in the extrusion process; the dispersion time of the poria cocos powder in a solvent is shortened, and the extraction yield of effective components such as total polysaccharides, total triterpenes and alcohol-soluble extracts in the poria cocos powder is remarkably improved; extrusion within the temperature range can particularly avoid reduction of total polysaccharide and alcohol-soluble extract components after extraction, and the extraction rate is increased.

The present invention discloses a fermentation method for Antrodia camphorata mycelium and an auxiliary composition for fermentation of Antrodia camphorata. The method provided by the present invention comprises the following steps: S1. subjecting an Antrodia camphorata strain to liquid fermentation for 60-120 hours; S2. adding precursors to the fermented Antrodia camphorata strain liquid fermentation broth, and continuing the fermentation for more than 120 hours to obtain Antrodia camphorata mycelium; the precursors being coenzyme Q0, p-hydroxybenzoic acid, linalool, and α-terpineol, and the fermentation broth after adding the precursors having a coenzyme Q0 content of 100-500 mg / L, a p-hydroxybenzoic acid content of 20-300 mg / L, a linalool content of 0.1-0.5 mg / L, and an α-terpineol content of 0.3-0.7 mg / L. This method adds precursor substances of specific composition and concentration to the liquid fermentation broth of Antrodia cinnamomea at a certain time and combines them with each other, which is beneficial to the secondary metabolism of Antrodia cinnamomea and promotes the synthesis of triterpenoid compounds, thereby increasing the yield. Compared with the existing technology, the yield of triterpenoid compounds produced by Antrodia cinnamomea mycelium is increased, the culture cycle is shortened, and it is more suitable for large-scale production.

The invention discloses a preparation method and application of a nano-composite for improving the bioavailability of a fat-soluble active substance curcumin by using a triterpenoid active micromolecule co-assembly. The invention aims to provide a preparation process of nanoparticles capable of loading active substances such as curcumin by utilizing the assembly characteristic of triterpenoid micromolecules and screening the combination proportion of materials. The preparation method is simple, convenient and low in cost. The nano-particles prepared by the method are spherical, uniform in particle size, and good in stability, water solubility and antioxidant activity; and the drug loading capacity of the nanoparticles encapsulated with the curcumin is 20.42%. According to the invention, high loading capacity of nutrient substances in the nano drug loading system is realized, and the bioavailability of polyphenol nutrient substances is improved.

Pentacyclic triterpene weight loss agents are provided herein. Also provided are pharmaceutical formulations containing a therapeutically effective amount of one or more of the weight loss agents, or pharmaceutically acceptable salts or prodrugs thereof, in combination with one or more pharmaceutically acceptable excipients. The pharmaceutical formulations can be administered to a pre-obese, obese, or morbidly obese patient to induce weight loss, reduce body fat, reduce food intake, improve glucose homeostasis, prevent obesity, or a combination thereof. The weight loss agents can also be co-administered with leptin or a leptin analog.

The present invention provides triterpenoid compounds in Ganoderma sinense fermented mycelia. The structural formulas of the triterpenoid compounds are Structural Formulas 1, 3, 4, and 7. The present invention also provides the application of the triterpenoid compounds in Ganoderma sinense fermented mycelia as a drug for preventing or treating inflammation; the triterpenoid compounds in the Ganoderma sinense mycelia are one or more of the compounds with Structural Formulas 1-8. The present invention for the first time conducts an anti-inflammatory activity test on the triterpenoid compounds isolated and purified from Ganoderma sinense mycelia, and finds that the compounds with Structural Formulas 1-8 can all inhibit the release of TNF-α and IL-6, and have significant anti-inflammatory activity.

Provided is the use of a traditional Chinese medicine composition in preparation of a drug for preventing major adverse cardiac and cerebrovascular events after percutaneous coronary intervention and ameliorating myocardial injury and vascular endothelial dysfunction after percutaneous coronary intervention. The traditional Chinese medicine composition is composed of total salvianolic acid and total hawthorn triterpene acid.

The invention relates to the field of lucid ganoderma cultivation, and particularly discloses a method for increasing the content of ganoderma triterpenes in lucid ganoderma, which comprises the following steps: S1, preparing a culture medium; step S2, basswood manufacturing; step S3, inoculation; step S4, mycelium culture; step S5, burying soil and growing ganoderma lucidum; step S6, forming; from two aspects, firstly, a fermentation accelerant is added during preparation of the culture medium, and the fermentation accelerant is prepared by mixing calcium lactate, salicylic acid, a citrate-phosphate buffer solution and hydroxypropyl-beta-cyclodextrin according to a specific mass ratio; secondly, in the soil burying and ganoderma lucidum growing process, culture medium soil rich in fermentation extract is adopted for culture, the fermentation extract is prepared into a fermentation substrate through compound bacteria fermentation by taking Chinese wolfberry residues, liquorice residues and astragalus membranaceus residues as raw materials, and the content of ganoderma lucidum triterpenes in ganoderma lucidum is increased through the synergistic effect of all the substances.