587 results about "Triterpene" patented technology

The invention discloses a ganoderma lucidum fermentation liquid capable of improving the content of total triterpenes and immunity and a preparation method and application thereof, the preparation method comprises the following steps: heating a ganoderma lucidum liquid, and carrying out hot water extraction to obtain a ganoderma lucidum extracting liquid; heating the lucid ganoderma extracting solution to be boiled, after sterilization is finished, after the lucid ganoderma liquid is cooled, adding lactic acid bacteria powder in a specific proportion for fermentation to obtain lucid ganoderma fermentation liquid; and carrying out solid-liquid separation on the fermentation liquor to obtain fermentation supernate, and sterilizing the ganoderma lucidum fermentation liquor to obtain the sterilized ganoderma lucidum fermentation liquor. According to the preparation method disclosed by the invention, the content of total triterpenes in the ganoderma lucidum fermentation liquor is increased, the immunomodulatory activity of the ganoderma lucidum product can be enhanced, and the ganoderma lucidum product has relatively high effects of resisting oxidation, improving the activity of phagocytes, improving the activity of acid phosphatase, improving the immunity and the like, and has a wide application prospect and market potential.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a centella asiatica glycosyl transferase gene in catalyzing glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the protein coded by the gene can catalyze the C-28 site of asiatic acid and madecassic acid to perform glucose group modification so as to generate asiatic acid monoglucoside and madecassic acid monoglucoside, and preferentially catalyzes madecassic acid. By constructing a metabolic regulation network, the genes are widely and accurately screened, and the glycosylation process of catalyzing asiatic acid and madecassic acid is analyzed; an important reference basis is provided for improving the glycosylation efficiency of asiatic acid and madecassic acid and the yield of asiaticoside and madecassoside in the in-vitro synthesis and industrial production process of the centella asiatica triterpenoid saponin, and an important theoretical support is provided for large-scale production of the centella asiatica active saponin.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention relates to the technical field of concrete admixtures, and discloses a nano rebound inhibitor for shotcrete and a preparation method thereof. The components, measured by weight, include 400-600 parts of nano microspheres, 400-550 parts of nano calcium carbonate, 10-30 parts of sodium carbonate, 1-10 parts of sodium aluminate, 10-30 parts of alum, 18-40 parts of polycarboxylic acid water reducer, and 0-0.5 parts of triterpenoid saponin; the nano microspheres are hydrated calcium silicate with silicon dioxide as the core material and colloid as the shell material, and the colloid is doped with a lithium source. The nano rebound inhibitor of the present application can significantly improve the state of shotcrete and greatly reduce the rebound amount of concrete, thereby further saving construction costs and accelerating construction accuracy. By utilizing the characteristics of the mineral admixture in the nano rebound inhibitor, the compactness of the concrete can be improved, and the durability of the shotcrete can be further improved.

The invention relates to the technical field of pharmaceutical preparations, in particular to a multiple nano emulsion adjuvant and a preparation method thereof. The multiple nano emulsion adjuvant is prepared from 2.73 to 6.37 g of an oil phase matrix based on 100 g of a raw material system, 0.10 to 0.25 g of a sterol compound; 0.212 to 0.424 g of a nonionic surfactant; 0.00050 to 0.00200 g of a triterpenoid saponin type active adjuvant; 0.010 to 0.020 g of an anionic polymer modifier; 0.005 to 0.015 g of a cationic polymer modifier; 70-90 g of a buffer solution; the total mass of the raw materials is 100 g. The nano emulsion adjuvant prepared by the invention can be stored for 6 months at 2-8 DEG C under a low sterol condition, the particle size change does not exceed + 5%, no crystallization or layering is generated, and the stability of the active adjuvant is remarkably improved.

The invention provides an application of a pentacyclic triterpenoid compound targeting hydroxyl steroid 17-beta dehydrogenase 4. The pentacyclic triterpenoid compound can effectively relieve or treat central nervous system diseases (inflammatory diseases), including central nervous system degenerative diseases or chronic central inflammatory diseases. The pentacyclic triterpenoid compound can maintain the structural stability or activity of hydroxysteroid 17-beta dehydrogenase and maintain the stability or activity of peroxisome, thereby relieving or treating central nervous inflammation. The pentacyclic triterpenoid compound can target hydroxysteroid 17-beta dehydrogenase 4, inhibit neuroglial cell excessive activation related neuroinflammation, relieve neuron damage or death speed and improve a central nervous system cell metabolism microenvironment.

The invention belongs to the technical field of food microorganisms, and particularly discloses a ganoderma lucidum mutant strain capable of producing polysaccharides and triterpenoids at high yield. The strain is named as Ganoderma lucidum in Latin, the preservation number is CGMCC NO: 41680, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is December 4, 2024. The strain is named as Ganoderma lucidum, the preservation number is CGMCC NO: 41680, and the preservation number is CGMCC NO: 41680. The polysaccharide content in the ganoderma lucidum spore powder produced by the ganoderma lucidum mutant strain is more than or equal to 8%, and the total triterpenoid content is more than or equal to 12%; a culture medium formula of the ganoderma lucidum mutant strain comprises potato leachate, glucose and monopotassium phosphate, the culture temperature is 25-28 DEG C, and the pH value is 5.5-6.5. The ganoderma lucidum mutant strain is high in genetic stability, the content of active ingredients is remarkably higher than that of parent strains, and the ganoderma lucidum mutant strain is suitable for large-scale fermentation production.

The invention relates to a poria cocos extract and a preparation process thereof. According to the invention, ethanol and N-methyl pyrrolidone are used as extraction solvents to prepare the poria cocos extract, and the extract is rich in poria cocos triterpenes and polysaccharides. Experimental results show that the obtained extract has a remarkable treatment effect on gall-stone model mice, the number of cholesterol crystals in gallbladders of the mice can be remarkably reduced, the content of cholesterol in the gallbladders can be reduced, and the content of phospholipid and bile acid in the gallbladders can be effectively increased.

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a radix puerariae fermented product with a blood pressure reducing effect and a preparation method thereof. The preparation method comprises the following steps: (1) adding water into kudzuvine root powder, uniformly mixing, and carrying out ultrasonic treatment to obtain a mixed solution; (2) sterilizing the mixed solution to obtain a sterilized solution; and (3) adding a leavening agent into the sterilized solution, fermenting at 25-35 DEG C for 24-72 hours to obtain a fermentation solution, and performing solid-liquid separation on the fermentation solution to obtain a fermentation supernatant, namely a fermentation product. According to the method, the content of puerarin, genistein and total triterpenes in the radix puerariae fermentation liquor can be increased, and a very good blood pressure reducing effect is achieved.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention provides a traditional Chinese medicine composition and application thereof, the traditional Chinese medicine composition contains total salvianolic acid and total triterpenic acid of hawthorn, and the traditional Chinese medicine composition has an excellent treatment effect on prevention or treatment of cerebrovascular diseases, especially ischemic cerebrovascular diseases.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The invention discloses a triterpenoid saponin compound derived from gynostemma pentaphylla and application of the triterpenoid saponin compound in preparation of an anti-fatigue product. The triterpenoid saponin compound provided by the invention is selected from one of the following compounds 1 to 29. The invention further provides application of the triterpenoid saponin compound shown in the general formula I in preparation of anti-fatigue products. Experiments show that the triterpenoid saponin compound derived from the gynostemma pentaphylla has remarkable anti-fatigue activity and can be used for preparing anti-fatigue products.

The invention belongs to the technical field of medicine, and discloses a sea cucumber and ganoderma lucidum immune steady-state compound and a composite enzymatic hydrolysis targeted liposome co-delivery preparation process thereof.The sea cucumber and ganoderma lucidum immune steady-state compound is composed of sea cucumber, ganoderma lucidum, American ginseng, dried oyster meat, fructus lycii and perilla leaves; the ganoderma triterpenes release free polysaccharides and are combined with a liposome intestinal targeted delivery system, so that the absorption rate of the polysaccharides is greatly improved to 18-22% from 5% in the traditional process, the ganoderma triterpenes are emulsified and entrapped through a liposome double-layer membrane, and meanwhile, the permeation promotion effect of perillaldehyde in the perilla leaf volatile oil is supplemented, so that the transmembrane transport efficiency is remarkably enhanced, the absorption rate is improved to 12-15% from 2%, and the bioavailability is improved. The zinc element decomposes oyster zinc protein into free zinc ions through a composite enzymolysis technology, and the free zinc ions and vitamin C form a stable chelate, so that the antagonism of intestinal tracts is reduced, the zinc absorption rate is increased from 20% to 45-50%, and a solid foundation is laid for fully playing a compound immune regulation effect.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention discloses a preparation method of a ganoderma spore oil grifola frondosa soft capsule based on a cold preparation method. The preparation method comprises the following steps: firstly, carrying out high-speed shearing and nanocrystallization treatment on grifola frondosa extract, selenium-enriched yeast, squalene and ganoderma lucidum spore oil for later use; and then melting gelatin, glycerol, water and cocoa shell extract at low temperature to obtain a gelatin solution, and finally preparing the soft capsule. According to the invention, on the basis of ensuring sufficient dissolution of the gelatin, the influence of the traditional gelatin melting temperature on the gel strength, the viscosity, the three-dimensional structure, the production stability and other performances and the application effect can be effectively avoided. Meanwhile, the ganoderma lucidum spore oil, squalene, grifola frondosa extract and selenium-enriched yeast are ingeniously combined, so that the content of a landmark component triterpenoid compound in the content is remarkably increased, and the metabolic detoxification capability of the liver and the anti-inflammatory and immune regulation effects of the body are enhanced.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The invention discloses a preparation method of an alkali aggregate inhibitor based on lithium slag and phosphorus slag multi-solid-waste materials, common concrete doped with the alkali aggregate inhibitor and a preparation method of the common concrete. The preparation method of the alkali aggregate inhibitor comprises the following steps: firstly weighing 25-50 parts of lithium slag powder, 30-40 parts of phosphorus slag powder, 15-25 parts of silicon powder, 5-10 parts of sodium sulfate and 0.05-0.2 part of triterpenoid saponin, mixing the pretreated lithium slag powder and phosphorus slag powder with the silicon powder, sodium sulfate and triterpenoid saponin, and uniformly stirring to obtain the alkali aggregate inhibitor based on the lithium slag and phosphorus slag multi-solid waste materials. The alkali aggregate inhibitor is prepared by taking the lithium slag powder, the phosphorus slag powder, the silicon powder, the sodium sulfate and the triterpenoid saponin as raw materials, and then 10-20wt% of cement is replaced by the alkali aggregate inhibitor in equal mass, so that not only can the generation of alkali aggregate be inhibited, but also the compressive strength, the impermeability, the freezing resistance and the like of the concrete can be improved.

The invention discloses a pharmaceutical composition for treating or improving Parkinson's disease and application of the pharmaceutical composition. The triterpenoid saponin compounds and the nervonic acid in different proportions are combined, and experiments prove that multiple indexes of Parkinson mice can be remarkably improved when the triterpenoid saponin compounds and the nervonic acid are combined in a specific proportion, and the effect of synergistically improving the Parkinson disease or the progress of the Parkinson disease is achieved. Therefore, the pharmaceutical composition disclosed by the invention can be used for more effectively treating or improving the Parkinson's disease, and has a wide application prospect in the field of treatment of the Parkinson's disease.

The invention discloses an engineered glycosyl transferase and application thereof in efficient preparation of triterpenoid saponin. The engineered glycosyl transferase is obtained by mutating serine at the 15th site of UGT74AC1 enzyme into alanine, mutating histidine at the 47th site into aspartic acid, mutating leucine at the 48th site into valine, mutating alanine at the 180th site into serine, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid The mutant is obtained by mutating serine at the 332 site into proline, phenylalanine at the 367 site into tryptophan and lysine at the 420 site into arginine. According to the invention, the glycosyl transferase with high catalytic activity is obtained by modifying an enzyme engineering technology, and good application of the glycosyl transferase in synthesis of triterpenoid saponin is realized. The glycosyl transferase disclosed by the invention can also be coupled with sucrose synthase, or coupled with N-acetylhexosamine kinase and N-acetylglucosamine-1-uridine phosphate transferase, and acetylglucosamine is used as a glycosyl donor, so that cascade production of triterpenoid saponin with lower cost and higher efficiency is realized.

The invention relates to the technical field of preparation methods of nutritional products, in particular to a preparation method of ganoderma lucidum spore oil with a liver regulating effect. Comprising the steps of preparing ganoderma lucidum spore powder, extracting ganoderma lucidum spore oil and enriching the ganoderma lucidum spore oil. Ganoderma lucidum blocks are treated through an ultrasonic extraction technology, ganoderma lucidum spore powder is prepared through a spray drying method, n-hexyl alcohol is used for soaking extraction, high-purity ganoderma lucidum spore oil is obtained through vacuum concentration and molecular distillation, and residual solvent is removed through film evaporation. Active ingredients such as triterpenoids and unsaturated fatty acid are further enriched by using supercritical CO2 extraction; the extraction efficiency and the purity of active ingredients of the ganoderma lucidum spore oil are remarkably improved, the obtained ganoderma lucidum spore oil has remarkable effects in the aspects of reducing liver toxicity indexes (ALT and AST) and enhancing antioxidant enzyme activity (SOD and GSH-Px), the operation controllability is high, the ganoderma lucidum spore oil is suitable for large-scale industrial application, and technical support is provided for research and development of liver protection drugs and functional food.

The invention discloses a strong-absorption water-soluble lutein compound preparation and application thereof in food with an eyesight protection function. The xanthophyll-dipotassium glycyrrhizinate water-soluble granules are water-soluble granules formed by molecular self-assembly of xanthophyll, dipotassium glycyrrhizinate, triterpenic acid, protein and dietary polysaccharide. Compared with untreated xanthophyll, the xanthophyll compound preparation with strong absorption has the advantages that the xanthophyll has good water solubility and stability, the intestinal absorption efficiency is improved by 20-35 times, and eyesight protection can be effectively realized. Meanwhile, the embedding rate is high, the safety is good, and the production cost is low.

The present invention discloses a preparation method of Ganoderma lucidum spore oil and Grifola frondosa soft capsules based on a cold preparation method. First, Grifola frondosa extract, selenium-enriched yeast, squalene, and Ganoderma lucidum spore oil are subjected to high-speed shear nano-processing and then set aside; subsequently, gelatin is subjected to low-temperature gelation with glycerol, water, and cocoa shell extract to obtain a gelatin solution, and finally, soft capsules are prepared. While ensuring that gelatin is fully dissolved, the present invention effectively avoids the impact of traditional gelation temperature on gel strength, viscosity, three-dimensional structure, production stability, and other properties and application effects. At the same time, the Ganoderma lucidum spore oil and squalene, Grifola frondosa extract, and selenium-enriched yeast are cleverly combined to significantly increase the content of triterpenoid compounds, a characteristic component in the contents, thereby enhancing the liver's metabolic detoxification ability and the body's anti-inflammatory and immunomodulatory effects.

The invention provides a preparation method and application of lucid ganoderma nano powder, a lucid ganoderma polysaccharide extract or a lucid ganoderma triterpenoid extract, and belongs to the technical field of lucid ganoderma processing and preparation. The preparation method of the lucid ganoderma nano powder comprises the following steps: airing lucid ganoderma sporocarp, cutting into lucid ganoderma particles, and crushing the lucid ganoderma particles to obtain lucid ganoderma coarse powder; drying the ganoderma lucidum coarse powder at constant temperature, and performing superfine grinding on the dried ganoderma lucidum coarse powder to obtain the ganoderma lucidum powder. The ganoderma lucidum nano powder is successfully prepared through superfine grinding, the content of heavy metal in the ganoderma lucidum nano powder is low, the content of polysaccharide and the content of triterpenes measured in the ganoderma lucidum polysaccharide extract and the ganoderma lucidum triterpenes extract obtained through extraction of the ganoderma lucidum nano powder are high, and the antioxidant activity of the ganoderma lucidum polysaccharide extract and the antioxidant activity of the ganoderma lucidum triterpenes extract are high; further research finds that the ganoderma triterpenoid extract has remarkable activity of inhibiting brain glioma. Various functional products are prepared from the ganoderma lucidum nano powder, and the ganoderma lucidum nano powder has important significance and application value.

The invention relates to the field of hericium erinaceus strains, and discloses a wild coral hericium erinaceus strain with high yield of triterpenes and application, the wild coral hericium erinaceus strain with high yield of triterpenes is preserved in China General Microbiological Culture Collection Center on August 13, 2024, the strain is Hs, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the postcode is 100101, the strain number is Hs, and the preservation number is CGMCC NO.41435. The culture medium is improved by applying liquid fermentation culture, so that the production period of the coral hericium erinaceus is effectively shortened, and the yield is increased; the cultivation medium is optimized, radix tetrastigme and mulberry branch powder are added to serve as the cultivation medium, so that the content of triterpenes is increased, sporocarp is pure white and good in commodity, the yield and quality are effectively improved, a more efficient coral hericium erinaceus cultivation mode is provided, the production cost of coral hericium erinaceus is greatly reduced, and the coral hericium erinaceus cultivation method is suitable for large-scale popularization and application. The yield and the quality of the coral hericium erinaceus are improved.

The invention relates to edible and medicinal fungi, in particular to a new strain of ganoderma leucocontextum and application thereof. The preservation number of the ganoderma leuconium RK001 (Ganoderma leuconium RK001) is CCTCC (China Center for Type Culture Collection) NO: M20232555, and the preservation number of the ganoderma leuconium RK001 is CCTCC NO: M20232555. The new strain Ganoderma leucoderma RK001 has the advantages of high content of crude polysaccharides and total triterpenes, short growth cycle and high yield, is suitable for artificial cultivation, and has high popularization value.

The invention discloses a preparation method and application of a rosa roxburghii tratt leaf extract for improving sleep. Roxburgh rose leaves are subjected to heating reflux extraction, ethyl acetate extraction, separation and purification to obtain the roxburgh rose leaf extract. The rosa roxburghii tratt leaf active extract is rich in triterpenes and flavones, has remarkable sleep improvement activity, and can be used for preparing medicines and auxiliary medicines for improving sleep or used as a health food additive; the preparation method is simple and easy to implement, low in cost and easy for industrial production, and has high application value and good application prospect.

The invention relates to the technical field of biological medicines, and discloses a preparation method of ganoderma lucidum wall-broken superfine powder for female insomnia, which is characterized in that thick ganoderma lucidum slices are subjected to spray moistening, hot-pressing steam heating, ultrasonic treatment, microwave drying and other process treatments, so that the contents of polysaccharides, triterpenes and sterols are increased; and the release rate of the active ingredients is also obviously improved. According to the technology, the structure of the cell wall of the ganoderma lucidum mycelium is destroyed, so that active ingredients such as polysaccharide, triterpenes and sterol originally existing in ganoderma lucidum can be easily extracted and released. Ganoderma lucidum cells are loosened and broken, polysaccharide in a combined state can be changed into a free state, and the content of ganoderma lucidum polysaccharide can be greatly increased. The cell-broken ganoderma lucidum wall-broken superfine powder can be directly taken in digestive tracts to quickly release active ingredients in ganoderma lucidum to the maximum extent, and the effect of improving female insomnia by the superfine powder is improved.