219 results about "Lycopersene" patented technology

The invention discloses a high-yield lycopene recombinant rhodobacter sphaeroides strain as well as a construction method and application thereof, and belongs to the technical field of biosynthesis and biological energy sources, the construction method comprises the following steps: knocking out a crtC gene of rhodobacter sphaeroides by using a homologous recombination method to obtain a recombinant strain RS01; the method comprises the following steps: on the basis of a strain RS01, replacing a crtI3 gene on a rhodobacter sphaeroides genome with a crtI4 gene (from rhodospirillum rubrum) by using a homologous recombination method to obtain a recombinant strain RS02; the method comprises the following steps: connecting crtB and crtE genes to a pYYDT vector to construct a plasmid pYYDT-crtB-crtE, transferring the plasmid pYYDT-crtB-crtE into escherichia coli S17, and then introducing the plasmid pYYDT-crtB-crtE into a recombinant rhodobacter sphaeroides RS02 in a conjugational transfer manner to obtain a recombinant strain RS03. The recombinant rhodobacter sphaeroides constructed by the invention can be used for efficiently producing lycopene.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention relates to the technical field of active peptides, and discloses a whitening and anti-wrinkle protein active peptide composition as well as a preparation method and application thereof. The whitening and anti-wrinkle protein active peptide composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of elastin peptide, 11 to 14 parts of fish collagen peptide, 8 to 10 parts of fish maw small-molecule active peptide, 5 to 10 parts of cubilose peptide, 5 to 10 parts of sea cucumber peptide, 2 to 6 parts of Chinese fevervine herb active peptide, 1 to 3 parts of zingiber corianum active peptide and 1 to 5 parts of lycopene. According to the whitening and anti-wrinkle protein active peptide composition and the preparation method and application thereof, small-molecule active peptide in the prepared protein active peptide composition is beneficial to absorption of dermal cells, and meanwhile, through the synergistic effect of multiple active ingredients, the whitening and anti-wrinkle effects are effectively achieved, the skin quality is improved, and aging is delayed.

The invention discloses a lycopene / starch compound, a vegetarian sausage as well as a preparation method and application of the vegetarian sausage, and belongs to the technical field of foods. The preparation method of the lycopene / starch compound comprises the following steps: (1) mixing starch and a phosphate buffer solution, gelatinizing, gelling, cooling, adding pullulanase, hydrolyzing, stopping hydrolysis, adding excessive ethanol, centrifuging, and freeze-drying in vacuum; (2) adding into water, heating and cooling to obtain starch suspension for later use; (3) dissolving lycopene in dimethyl sulfoxide, and carrying out ultrasonic dissolution promotion to obtain a lycopene solution; and (4) heating the lycopene solution, mixing the heated lycopene solution with the starch suspension, stirring, naturally cooling to room temperature, refrigerating, centrifuging, collecting precipitate, carrying out vortex oscillation, flushing, and carrying out vacuum freeze drying to obtain the lycopene starch. The lycopene / starch compound prepared by the invention can obviously improve the quality of the vegetarian meat sausages and improve the value of the vegetarian meat sausages.

The invention relates to the technical field of food processing, in particular to fish-derived protein gel special for 3D printing dysphagia and having a customized digestion characteristic and a lycopene release function as well as a preparation method and application of the fish-derived protein gel. The lycopene-loaded IN / KGM gel system is constructed by combining a food additive manufacturing technology and a dynamic old people digestion model, and the rheological property and swallowing safety of the gel are optimized by regulating and controlling the ratio of IN to KGM; the customized digestion characteristics of the gel and the adaptability of the gel to the digestion function of the old people are researched by utilizing an in-vitro dynamic digestion model of the old people; the loading and releasing behaviors of lycopene in a gel system are explored, the bioaccessibility of lycopene in the gastrointestinal digestion process of old people is evaluated, the interaction mechanism of IN and KGM in the gel forming and digestion process is disclosed, and a theoretical basis and technical support are provided for developing personalized nutritious food suitable for the old people with dysphagia.

The invention relates to an application of a genetic transformation method of populus sibirica and a CRISPR / Cas9 gene editing method in plant breeding, and belongs to the technical field of plant molecular breeding. The method comprises the following steps: (1) soaking an explant in an infection bacterium solution; (2) spreading on a CM1 culture medium; (3) transferring the explant to a CM2 culture medium for 4-5 weeks; (4) transferring the calluses to a CM3 culture medium until adventitious buds are obtained; (5) transferring the callus with the adventitious buds to a CM4 culture medium until cluster buds are obtained; and (6) cutting off clustered seedlings, and inserting the clustered seedlings on a CM5 culture medium. According to the application of the genetic transformation and CRISPR / Cas9 gene editing method of the poplar in plant breeding, a target gene is transferred into a poplar body to obtain a positive plant, meanwhile, a poplar phytoene dehydrogenase (PDS) gene is taken as an example, a poplar mutant of which the PDS gene is knocked out is successfully obtained by applying a CRISPR / Cas9 gene editing technology, and the method is suitable for plant breeding. And a technical support is provided for application of a CRISPR / Cas9 gene editing technology in molecular breeding of the populus sibirica.

The invention relates to the technical field of food processing, in particular to 3D printing flaxseed gum fish-source protein gel adaptive to elderly swallowing and bionic digestion response as well as a preparation method and application of the 3D printing flaxseed gum fish-source protein gel. According to the present invention, by constructing the lycopene-loaded flaxseed gum-myofibrillar protein composite gel system, the multi-scale structure regulation and the lycopene precise delivery are achieved, and the function adaptation mechanism of the lycopene in the 3D printing swallowing food is systematically clarified; the invention focuses on a molecular mechanism that flaxseed gum molecule acting force rearrangement drives myofibrillar protein secondary structure transformation, and combines rheological recovery characteristics of LFG gel under a dynamic shearing condition and a cross-scale regulation effect of swallowing kinetics, and further discloses a lycopene release behavior of the LFG gel in a process of simulating dynamic digestion of old people. From the synergetic perspective of molecular interaction, rheological response and digestive behavior regulation, reference is provided for the development of elderly special dietary food with swallowing suitability, accurate nutrition delivery and sensory palatability.

The invention discloses a method for detecting the protein catalytic function of carrot lycopene beta-cyclase, and belongs to the technical field of protein catalytic function detection. According to the method for detecting the catalytic function of the carrot lycopene beta-cyclase protein, escherichia coli is used as a heterologous expression host, a lycopene substrate is accumulated in the escherichia coli by expressing pACCRT-EIB plasmids, then lycopene epsilon-cyclase protein and lycopene beta-cyclase are constructed on the same carrier for co-expression, and the lycopene beta-cyclase protein is obtained. A monocyclic carotene intermediate product in the process of converting lycopene into carotene is introduced into a reaction system so as to detect the substrate preference and functional difference of two lycopene beta-cyclase isoenzymes. The method is easy to operate, expression in an escherichia coli system is convenient and rapid, various lycopene beta-cyclase catalytic substrates except lycopene can be provided, and the catalytic function of lycopene beta-cyclase protein can be identified more accurately.

The invention discloses a saccharomyces cerevisiae strain for de novo synthesis of fucoxanthine precursor neoxanthine and application of the saccharomyces cerevisiae strain, and belongs to the technical field of biology. According to the recombinant saccharomyces cerevisiae disclosed by the invention, the 3-hydroxy-3-methylglutaryl coenzyme A reductase, the isopentenyl pyrophosphate isomerase, the farnesyl pyrophosphate synthase and the endoplasmic reticulum size regulation factor are expressed in an enhanced manner; according to the method, geranyl geranyl diphosphate synthase, phytoene dehydrogenase, 15-cis-phytoene synthase, bifunctional lycopene cyclase / phytoene synthase, beta-carotene hydroxylase, zeaxanthin epoxidase truncated mutant and violaxanthin deep oxidase-like protein are subjected to heterologous expression, so that the zeaxanthin deep oxidase-like protein is obtained; the ROX1 and GAL80 genes in the saccharomyces cerevisiae are knocked out, and the expression of squalene synthase ERG9 is down-regulated. When the strain is subjected to shake flask fermentation, the contents of neoxanthine and violaxanthin of the strain respectively reach 1.78 mg / L and 26.77 mg / L, so that the strain has a wide application prospect.

The invention belongs to the technical field of enzyme mutants, and discloses a mutant of lycopene cyclase and application of the mutant, glutamic acid at the 321 site of wild type lycopene cyclase which is derived from arabidopsis and has an amino acid sequence as shown in SEQ ID NO.1 is mutated into lysine, phenylalanine at the 319 site of the wild type lycopene cyclase is mutated into leucine, cysteine at the 323 site of the wild type lycopene cyclase is mutated into alanine, and the mutant of the lycopene cyclase is obtained. 1, and the lycopene cyclase mutant with the amino acid sequence as shown in SEQ ID NO. 2 is obtained. BTS1, CrtB and CrtI are integrated into a saccharomyces cerevisiae BY4741 strain, and a chassis strain ZA1 for stably producing alpha-carotene precursor lycopene is constructed; mutant expression plasmids are constructed, positive clone strains are screened out, and the epsilon-cyclization ability of mutant enzymes is remarkably higher than the beta-cyclization ability; the method comprises the following steps: by taking lycopene as a substrate, constructing an expression vector lipid droplet surrounding protein PET10; gene PAH1, DGA1 and Cat2 related to TAG synthesis and perilipid droplet protein PET10 are constructed on an expression vector and converted into ZA1, and when lycopene is used as a substrate, the efficiency of catalytic production of alpha-carotene is improved.

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition and application of the traditional Chinese medicine composition in preparation of drugs for improving sleep disorder and treating mood disorder or cognitive disorder. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by mass: 20-30 parts of lycium ruthenicum, 30-40 parts of nitraria fruit, 15-25 parts of gastrodia elata, 13-25 parts of hyperforin perforatum and 2-6 parts of lycopene. The traditional Chinese medicine composition provided by the invention is prepared from lycium ruthenicum, nitraria fruit, gastrodia elata, hyperforin perforatum and lycopene according to a specific ratio, and the composition can effectively relieve anxiety behaviors of chronic stress bound anxiety model mice through ways of resisting oxidation and inflammation, regulating expression of neurotransmitters and the like, prolong sleep time and improve sleep quality. The learning ability and the memory ability of cognitive impairment mice induced by scopolamine are improved, and the neuron density of brain tissues is increased, so that the neuroprotective effect is achieved.

The utility model relates to the field of lycopene content detection, in particular to a sampling device for lycopene content detection, which comprises an outer box body, a panel seat, a top sealing cover and an extraction barrel, a placing groove is arranged on the surface of the outer box body, the panel seat is matched with the right side of the placing groove, the extraction barrel is arranged on the inner side of the panel seat, and the top sealing cover is arranged on the top sealing cover. A through hole is formed in the top of the placement groove, and the top sealing cover is slidably connected with the through hole; the extraction barrel is installed on the panel base, through the split structure of the panel base and the outer box body, the extraction barrel can be conveniently taken out for sampling operation, meanwhile, the extraction barrel can be removed for cleaning, the cleaning effect is guaranteed, meanwhile, the crushing structure is installed on the top sealing cover, cleaning operation of a blade is facilitated, secondary use is facilitated, and the extraction barrel can be conveniently taken out for sampling operation. A filter screen and a filter plate are arranged on the inner side of the extraction barrel, solid-liquid separation operation after tomatoes are smashed is facilitated, liquid enters a bottom liquid storage cavity, solids are retained on the surface of the filter plate, cleaning is conducted by removing the filter plate, and convenience is improved.

The invention discloses a method for measuring the content of water-insoluble solids in a nanocrystallized lycopene liquid beverage, which comprises the following steps: acquiring spectral data of a sample by an infrared spectrometer, extracting spectral characteristics and constructing a spectral matrix; calculating a water-insoluble solid content value to form a numerical matrix; removing spectral noise by using a Savitzky-Golay convolution smoothing method, and calculating a second derivative spectrum; main characteristic components are extracted through a partial least square method, and a mathematical relationship model between spectral characteristics and the water-insoluble solid content is established; and finally, inputting sample spectrum data into the model, and predicting the content of water-insoluble solids. According to the method, the mathematical relationship model is established by combining the infrared spectrum technology with the partial least square method, so that the rapid and nondestructive detection of the water-insoluble solid content in the nanocrystallization lycopene liquid beverage is realized.

The present application discloses a spectral detection method suitable for detecting the lycopene content in agricultural products. The method is implemented by a spectrometer, and the spectrometer includes: a light source device, a converging device, a chopping device, a filtering device, a detection device and a processing device; the spectral detection method includes: exciting the light source device to generate light source light; the converging device converges the light source light into converged light; controlling and driving the chopping device to obtain detection light with a modulation frequency for irradiating the agricultural products; arranging a filtering device on the optical path of the detection light to obtain characteristic light; controlling the detection device to convert the optical signal of the characteristic light into an electrical signal; and controlling the processing device to output the analysis result of the substance content according to the electrical signal output by the detection device. The present application is beneficial in that: it provides a spectral detection method suitable for detecting the lycopene content in agricultural products, which can effectively detect the lycopene content in agricultural products.

The invention belongs to the technical field of food safety and quality detection, and provides a method for detecting lycopene content by paper-based surface enhanced Raman. The method comprises the following steps: reacting chloroauric acid with sodium citrate to obtain a gold nano solution, and carrying out centrifugal concentration on the gold nano solution to obtain a concentrated gold nano solution; mixing a lycopene-containing solution to be detected with the concentrated gold nano solution to obtain a sample solution; dipping the paper tip substrate in a sample solution, sequentially performing drying and lycopene Raman signal detection after dipping, and obtaining the content of lycopene in the to-be-detected solution according to a Raman signal detection result, the preparation method of the paper tip substrate comprises the following steps: sequentially cutting filter paper and sealing the filter paper with wax oil to obtain the paper tip substrate. The method not only can give full play to the advantages of the SERS technology, but also has the characteristics of high detection precision, high detection speed, good detection stability and repeatability and low detection cost, and is beneficial to wide application in the field of rapid detection.

The invention relates to a lycopene composition and an emulsion preparation thereof. The lycopene composition is prepared from lutein, beta-carotene, all-trans lycopene, 9-cis lycopene, 11-cis lycopene and 13-cis lycopene. The emulsion preparation also comprises an emulsifier, a co-emulsifier, an antioxidant and water, has good solubility in water, can obtain solutions with chromaticity of L30-35, a35-40 and b25-30, and is concentrated in tone value range and stable in tone coloring.

A heart boosting formulation comprises: L-carnitine, alpha-linolenic acid, citrulline, vitamin D3, lycopene, holy basil, cinnamon and beetroot.

The invention provides recombinant Escherichia coli for synthesizing carotene pigments. The recombinant Escherichia coli comprises the following multiple modifications: (a) introducing and expressing an mvaS gene, an mvaE gene and an mvk gene; (b) introducing and expressing a pmk gene and an mvd gene; and (c) introducing and expressing a crtB gene, a PDS gene, a crtI gene, a crtE gene, an idi gene and the like. According to the present invention, the recombinant Escherichia coli capable of highly producing the hexahydrolycopene, the phytoene or the lycopene is prepared, the target product yield is increased while the by-product yield is reduced, the bottleneck problem in the industrial production is solved, and the recombinant Escherichia coli can be used for the mass production of the hexahydrolycopene, the phytoene or the lycopene industry.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The present invention relates to a method for improving the antioxidant activity of Lactococcus lactis and its application. The method for improving the antioxidant activity of Lactococcus lactis is to construct a recombinant lactic acid bacterium that produces lycopene, thereby improving the antioxidant activity of Lactococcus lactis. The method for constructing the recombinant lactic acid bacterium that produces lycopene is to construct a lycopene heterologous synthesis plasmid, knock out the lactate dehydrogenase (LDH) gene in Lactococcus lactis or overexpress MVA pathway genes in strains of different sources to obtain the recombinant Lactococcus lactis, thereby achieving heterologous synthesis of lycopene by Lactococcus lactis. Compared with the prior art, the present invention constructs a recombinant lactic acid bacterium that produces lycopene, measures its own antioxidant activity and that in skim milk, and finds that the antioxidant activity of the recombinant Lactococcus lactis is more than 1 times that of the original strain, and the DPPH, ABTS and ·OH free radical scavenging abilities are significantly enhanced. The recombinant Lactococcus lactis is expected to be developed into an antioxidant probiotic product or fermentation agent.

The utility model relates to the technical field of lycopene production, in particular to a lycopene production ingredient blending device which comprises a blending box, a heating sleeve barrel is fixedly connected to the upper end of the blending box, a blending tank is fixedly connected to the interior of the heating sleeve barrel, a lifting air cylinder is fixedly connected to the position, located on the right side of the heating sleeve barrel, of the upper end of the blending box, and a connecting plate is fixedly connected to the output end of the lifting air cylinder; through the use of the stirring rod and the filter barrel I, ingredients are fully stirred and uniformly mixed at different depths, the stirring efficiency is improved, and meanwhile, the scraping blades fully clean and scrape the inner wall of the ingredient tank along with the rotation of the stirring blades, so that the adhesion of the materials is prevented, the mixing effect is improved, the heat transfer is promoted, and blended mixed liquid flows into the filter barrel II; the first filtering barrel rotates to drive the second filtering barrel to rotate synchronously, centrifugal force is generated to conduct double filtration on mixed liquid, the filtering precision is high, and the product quality of lycopene is effectively improved.

The invention provides mutagenic chlorella capable of realizing high yield of phytoene and application of the mutagenic chlorella, and belongs to the technical field of chlorella. The mutagenic algal strain SZU-C49 of the mutagenic chlorella pyrenoidosa is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66780, the preservation time is July 30, 2025, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The algal strain is obtained by carrying out chemical mutagenesis and screening on a mutagenic chlorella wild type common chlorella original strain. The mutagenic chlorella disclosed by the invention is cultured for 72 hours under an optimized culture condition, the content of phytoene in cells of the strain reaches 25799 mg / g (dry weight) and is more than 100 times that of an original strain, and the mutagenic chlorella can be widely applied to the fields of functional foods, health-care products, cosmetics, medicines and the like.

The invention discloses yarrowia lipolytica engineering bacteria for producing esterified astaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. According to the invention, acyltransferase ScDGAT2-1 from fission vibrio is integrated in a genome of a yarrowia lipolytica engineering strain DN20, diacylglycerol acyltransferase DGA1 and phosphatidic acid phosphatase PAH1 are knocked out, and the corn oil lipoprotein Oleosin and the acyltransferase ScDGAT2-1 from fission vibrio are subjected to fusion expression, so that the yarrowia lipolytica engineering strain DN20 is obtained. According to the present invention, the Yarrowia lipolytica engineering bacterium capable of producing esterified astaxanthin at a high yield is constructed by using SBT06-005 as a raw material and integrating beta-carotene hydroxylase CrtZ, endogenous truncated 3-hydroxy3-methylglutaryl coenzyme A reductase tHMG1, phytoene synthase CarRP and beta-isopropyl malate dehydrogenase LEU2, and the SBT06-005 can produce 166.6 mg / L esterified astaxanthin and 1097.0 mg / L total astaxanthin in a 5L fermentation tank.

The invention discloses a composition for improving sexual life and / or prostate symptoms and a preparation method thereof. The composition is prepared from the following components in parts by weight: 30 to 50 parts of algal polysaccharides, 15 to 25 parts of phospholipid, 8 to 12 parts of euphausia superba oil, 8 to 12 parts of magnesium oxide, 8 to 12 parts of lycopene, 2 to 4 parts of zinc gluconate, 1 to 3 parts of radix ginseng extract, 0.8 to 1.2 parts of flos sophorae immaturus extract, 0.8 to 1.2 parts of fructus rubi extract, 0.8 to 1.2 parts of mulberry fruit extract, 0.8 to 1.2 parts of fructus lycii extract and 0.8 to 1.2 parts of sodium selenite. By limiting the raw materials and the dosage, under the combined action of the raw materials, the prepared composition can improve erectile, ejaculation and anterior lacrimal gland symptoms, can improve the quality of sexual life, and has no side effect on the body.

The invention discloses a method for rapidly extracting industrial microorganism lycopene. Comprising the following steps: saponifying fermentation liquor, breaking bacteria, and extracting to obtain the lycopene. The method provided by the invention improves the efficiency of lycopene extraction, and overcomes the defects of low extraction rate, flammable and explosive properties and the like of the existing lycopene organic extractant. The method is favorable for improving the quality of the tomato pigment and is suitable for industrial production.

The invention provides recombinant saccharomyces cerevisiae for producing beta-carotene and application of the recombinant saccharomyces cerevisiae, and belongs to the field of genetic engineering. According to the invention, a beta-carotene metabolic pathway is constructed in saccharomyces cerevisiae, screening is carried out on a key gene lycopene cyclase gene in the metabolic pathway, and site-directed mutagenesis is carried out on lycopene cyclase derived from Rhizomucor circinelloides f. Lusitanius, so that a mutant is constructed. According to the invention, the constructed mutant is expressed in saccharomyces cerevisiae, so that the titer of beta-carotene is remarkably improved.

The invention discloses a bacterium-material heterozygote, a construction method thereof and a method for efficient carbon sequestration synthesis of lycopene, and belongs to the technical field of microbial electrosynthesis. According to the invention, the surface of engineering strain photosynthetic bacteria is modified with tannic acid modified ZIF-8 (TA ZIF-8) by using an in-situ growth method, so that the photosynthetic bacteria are promoted to capture CO2 in MES reaction and synthesize lycopene. According to the invention, the cell heterozygote which takes CO2 as a substrate and is used for synthesizing lycopene by providing reducing power through a biological cathode is constructed, so that the efficiency and yield of producing lycopene by MES carbon sequestration are effectively improved; mES reaction and yield test results show that under the optimal performance, 72h MES reaction can generate 2.062 mg / L lycopene, and the yield is increased by 29.2% compared with that of an unmodified engineering strain.

1. Name of the product in this design: Packaging Box (Lycopene Oleoresin Soft Capsules). 2. Application of this design: This design relates to a packaging box for lycopene oleoresin soft capsules. 3. The key design features of this product are the combination of pattern and color. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The design for which protection is sought includes color.

The invention provides a method for dynamically regulating and controlling the copy number of plasmids in saccharomyces cerevisiae based on RNA (Ribonucleic Acid) interference. According to the method, an orthogonal RNAi system is reconstructed, saccharomyces cerevisiae is used as a synthetic biological chassis with a dynamic programmable plasmid copy number, a heterologous RNAi mechanism from Ckstri saccharomyces cerevisiae is integrated, sequence-specific siRNA of a targeted plasmid coding selective marker is designed, a chemically induced gene dose control platform is established, and the gene dose control platform is used for controlling the gene dose. The dynamic regulation and control of the plasmid copy number are realized, so that the gene expression dosage is optimized, and the biosynthesis efficiency is improved. RNAi-mediated copy number regulation is applied to a carotenoid biosynthesis pathway, and compared with a static plasmid system, the lycopene titer is increased by 18.6 times. The method further enriches an anabolism regulation tool set, has huge potential in the aspect of improving the production performance of a microbial cell factory, and provides a new thought for metabolic engineering optimization in the field of synthetic biology.