356 results about "Chlorogenic acid" patented technology

The invention discloses a mucosal epithelial cell targeted oral ROS response type nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The oral ROS response type nano-enzyme comprises Ce-CDs carbon dots and a betaine polymer, and the betaine polymer is coated on the surfaces of the Ce-CDs carbon dots to form nano-particles; the Ce-CCDs carbon dots are prepared by taking a metal cerium source and chlorogenic acid as raw materials through a pyrolysis method. Through structural innovation and function integration, the prepared nano-enzyme shows outstanding advantages in the aspects of catalytic activity, targeted delivery, collaborative treatment, industrial application and the like, a new technical scheme is provided for efficient and safe treatment of inflammatory bowel diseases, and the nano-enzyme has remarkable technical innovation and clinical application value.

The invention discloses a preparation method of a fermented coffee pericarp fruit juice beverage. The coffee pericarp and the fruit juice are used as raw materials and are sequentially fermented by two strains, so that on the premise that the content of chlorogenic acid in the beverage is relatively high, the acerbity of the coffee pericarp is reduced, and the content of flavor substances with fruit flavor, flower flavor and the like is increased, so that the beverage not only has rich coffee flavor and fruit flavor, but also has soft ethanol flavor; the coffee pericarp is mellow and smooth in taste, meets diversified taste requirements of consumers, and provides a new thought for further development and utilization of coffee pericarp.

The present invention relates to a dandelion active component extraction process optimization method based on an I-WO-SVR model. The method comprises: acquiring factors to be predicted, wherein said factors comprise the ethanol concentration, ultrasonic time, ultrasonic temperature and ultrasonic power; and inputting said factors into an I-WO-SVR model, so as to acquire the extraction rate of chlorogenic acid from dandelion flowers and stems, wherein the I-WO-SVR model is obtained by using a training set to train a support vector regression (SVR) prediction model and using an improved walrus optimizer (I-WO) to optimize a penalty parameter and a nuclear parameter of the SVR prediction model, and the training set comprises original factors and chlorogenic acid extraction rates corresponding to the original factors. The present invention combines modern artificial intelligence technology with the field of traditional Chinese medicine to design a Chinese medicinal material active component extraction optimization method, has broad application prospects, and can provide new ideas for Chinese medicine extraction and intelligent industrial production thereof.

The invention relates to the technical field of daily chemical and skin external preparations, in particular to a six-dimensional synergistic effect-based acne removal and repair composition, which is prepared from the following active ingredients in parts by weight: 1 to 10 parts of sturgeon caviar extract, 0.1 to 10 parts of chlorogenic acid, 0.1 to 20 parts of 4-tert-butyl cyclohexanol and 0.1 to 20 parts of willow extract. The sturgeon caviar extract is prepared by supercritical CO2 fluid extraction, the willow extract is treated by a synthase process, the content of salicin is not less than 1.5 wt%, and at least one active component in the composition is subjected to supramolecular inclusion modification; the preparation method comprises the following steps: sequentially preparing the extracts and components, sequentially mixing, and finally carrying out an inclusion reaction. The composition constructs a full-link acne removal and skin health maintenance system through a six-dimensional synergistic effect of bacteriostasis, oil control, anti-inflammation, allergy relief, cutin dredging and repairing, has the characteristics of efficient acne removal, mildness, safety and low recurrence rate, is suitable for various skin types such as sensitive acne skin, and can be widely applied to acne removal products of various dosage forms.

The invention relates to the technical field of separation and extraction, and provides an extraction method and application of chlorogenic acid in honeysuckle. The invention relates to a method for extracting chlorogenic acid from honeysuckle, which comprises the following steps: mixing honeysuckle powder with an ethanol solution, and carrying out ultrasonic enzymolysis; carrying out suction filtration to obtain a supernatant A containing chlorogenic acid and medicine residues I, and adding an ethanol solution into the medicine residues I to carry out microwave extraction; performing suction filtration to obtain a filtrate B and medicine residues II, mixing the medicine residues II with a citric acid-sodium citrate buffer solution, adding a Fe3O4-coated GO-coated MOFs nano-catalyst, performing hydrothermal reaction, performing suction filtration to obtain hydrolysate, and combining the hydrolysate with the supernate A and the filtrate B to obtain a total extraction solution; concentrating the total extracting solution under reduced pressure, and recovering ethanol until the volume of the concentrated solution is about 1 / 6-1 / 8 of the original volume; and concentrating under reduced pressure to thick paste, and drying to obtain the chlorogenic acid. The extraction efficiency and yield of chlorogenic acid are improved, and the utilization rate of raw materials and the product purity are improved.

The invention provides a fingerprint spectrum and multi-component content determination method of a calamus-far nerve calming mixture, and relates to the field of quality control of traditional Chinese medicines. 31 common peaks are calibrated by establishing a fingerprint spectrum, and 24 common peaks can clearly belong to polygala tenuifolia. Meanwhile, synchronous content determination of seven components including chlorogenic acid, caffeic acid, ferulic acid, liquiritin, 2, 3, 5, 4 '-tetrahydroxy stilbene-2-O-beta-D-glucoside, 3, 6'-dimustard acyl sucrose and rosmarinic acid is realized. The method is good in precision, high in repeatability and stable and reliable in result, the medicine quality can be comprehensively and accurately reflected and controlled, and powerful support is provided for guaranteeing the safety and effectiveness of the calamus-far Ningmind mixture.

The invention relates to the technical field of toothpaste preparation, and particularly discloses bamboo leaf flavonoid toothpaste as well as a preparation method and application thereof. The bamboo leaf flavonoid toothpaste is prepared from the following raw materials in parts by weight: 26 to 30 parts of a wetting agent, 1.6 to 1.8 parts of a binding agent, 0.2 to 0.4 part of a sweetening agent, 20 to 24 parts of a friction agent, 1.2 to 1.6 parts of a surfactant, 0.3 to 0.5 part of a pH (Potential of Hydrogen) regulator, 2.4 to 2.8 parts of active microcapsules, 0.4 to 0.6 part of essence and 14 to 18 parts of de-ionized water. A core material of the active microcapsule is a nano-dispersion, a wall material of the active microcapsule is prepared from Arabic gum and chitosan through a complex coacervation method, the nano-dispersion is prepared from xanthan gum, a bamboo leaf flavone extract, xylitol, calcium chloride and modified pectin through a reaction, and the modified pectin is prepared by mixing degreased pectin and chlorogenic acid according to a specific proportion. Through the synergistic effect of the substances, the dispersibility and antibacterial and anti-inflammatory performance of the bamboo leaf flavonoid toothpaste are improved.

The invention discloses a coffee-flavor plant extract and a preparation method thereof, and the coffee-flavor plant extract is refined by using lophatherum gracile, Chinese olive, folium cortex eucommiae, dendrobium officinale, astragalus membranaceus, codonopsis pilosula, dandelion and liquorice as composite plant raw materials through multi-strain synergistic fermentation and oak barrel aging and curing technologies. The dissolution rate and bioavailability of various bioactive components such as chlorogenic acid, ginsenoside, curcumin and dendrobium polysaccharides are effectively improved through multi-strain synergistic fermentation. The oak barrel ageing endows the product with unique baking, nut and vanilla flavors, the taste is mellow, and the flavor characteristic of coffee is effectively simulated. The plant extract prepared by the invention is rich in various functional components, and can assist in improving the memory ability. Meanwhile, a healthy, caffeine-free and rich-flavor herbaceous plant beverage is provided for consumers, a new solution is provided for product development of plant extracts, and huge market potential and economic value are achieved.

Compositions and methods for providing a person with an exogenous and therapeutically effective supply of short chain fatty acids, chlorogenic acid, and, optionally, ketones are disclosed. The compositions include one or more short chain fatty acids (such as butyric acid, butyrate salts, propionic acid, propionate salts, acetic acid, or acetate salts); chlorogenic acid; and, optionally, one or more beta-hydroxybutyrate salts. The compositions and methods are useful for inducing ketosis and / or beta-oxidative activity in a subject. In addition, compositions that include concentrated forms of, and methods of using, chlorogenic acid (alone or in combination with short chain fatty acids and ketones) to induce ketogenesis in a subject are disclosed.

The invention relates to high-stability amoxicillin powder for livestock. 100 kg of the amoxicillin powder mainly comprises the following raw materials by weight: 10 to 30 kg of amoxicillin, 2.5 to 7.5 kg of potassium clavulanate, 0.6 to 0.7 kg of a hydroxypropyl-beta-cyclodextrin-baicalin and chlorogenic acid compound inclusion body, 1.8 to 2.4 kg of citric acid and calcium carbonate controlled release microspheres, 23.7 to 45 kg of oat beta-glucan, 18 to 25 kg of pre-gelatinized cassava starch, 3 to 4 kg of mannitol, 8 to 12.7 kg of silanized microcrystalline cellulose, and the balance of water. The coating comprises the following components by weight: 0.8 to 1.2 kg of nano silicon dioxide, 2.0 to 2.5 kg of hydroxypropyl methylcellulose and 0.6 to 1.0 kg of zinc diricinoleate. The powder is dried at low temperature, so that the degradation rate of beta-lactam rings is reduced, the stability of amoxicillin is improved, and the bioavailability of the medicine is improved.

The invention discloses construction and application of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum for integrally controlling a multi-component material basis of a traumatic injury bone-growing capsule, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and neochlorogenic acid, protocatechuic aldehyde, cryptochlorogenic acid, vanillic acid, rosmarinic acid, alkannic acid, berberine hydrochloride and salvianolic acid B are taken as reference substances; the fingerprint spectrum is successfully constructed by detecting different batches of traumatic injury bone-growing capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least eight components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be carried out on a plurality of characteristic components of the traumatic injury bone-growing capsule accurately, rapidly and simultaneously, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the traumatic injury bone-growing capsule.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The invention discloses a chlorogenic acid component detection method fused with deep learning, and relates to the technical field of chlorogenic acid. The method comprises the following steps: acquiring a chlorogenic acid hyperspectral image; clustering the chlorogenic acid hyperspectral image through a fuzzy C-means clustering algorithm based on a plurality of subclass centers to obtain a chlorogenic acid hyperspectral cluster, and calculating a chlorogenic acid spectral cluster feature vector; the method comprises the following steps: calculating mutual information of chlorogenic acid hyperspectral cluster feature vectors to obtain hyperspectral cluster feature weights; obtaining a chlorogenic acid hyperspectral cluster weighted vector by combining the chlorogenic acid hyperspectral cluster feature vector and the hyperspectral cluster feature weight; inputting the chlorogenic acid hyperspectral cluster weighted vector into a support vector machine regression model based on high-dimensional multiple scales, and outputting to obtain a chlorogenic acid concentration predicted value; and constructing a chlorogenic acid concentration diagram according to the chlorogenic acid concentration predicted value, calculating a concentration abnormal value through a sliding window method, and performing early warning if the concentration abnormal value is greater than a preset threshold value.

The invention relates to the technical field of hydrogel, in particular to a hydrogel system based on oxidized gastrodia elata stem polysaccharide as well as a preparation method and application of the hydrogel system. The preparation method comprises the following steps: performing water extraction and alcohol precipitation treatment on gastrodia elata stems to obtain a gastrodia elata stem polysaccharide sample; the method comprises the following steps: carrying out oxidation treatment on a gastrodia elata stem polysaccharide sample by using sodium periodate, and introducing an aldehyde group into the gastrodia elata stem polysaccharide sample to obtain an oxidized gastrodia elata stem polysaccharide sample; activating chlorogenic acid by using an activating agent to obtain activated chlorogenic acid; the preparation method comprises the following steps: grafting chitosan and activated chlorogenic acid to obtain a graft of chitosan grafted chlorogenic acid; and carrying out Schiff base reaction on a cross-linking accelerant, the oxidized gastrodia elata stem polysaccharide sample and the graft of chitosan grafted chlorogenic acid to obtain the hydrogel system containing the oxidized gastrodia elata stem polysaccharide. According to the preparation method, the replacement frequency of a hydrogel system is reduced by optimizing the structural stability and long-acting moisturizing adhesiveness of the hydrogel, and meanwhile, the recovery effect of the hydrogel system is improved by virtue of the synergistic effect of various active components.

The invention provides an application of chlorogenic acid as a vaccine adjuvant. Research finds that when chlorogenic acid is used as the adjuvant of the vaccine, the vaccination effect of the vaccine can be effectively enhanced, meanwhile, humoral immune response and cellular immune response mediated by the vaccine are improved, and better immune protection is provided. The chlorogenic acid is used as the adjuvant of the vaccine, so that better immune protection can be provided, and the chlorogenic acid is low in extraction cost, easy to digest and absorb by a human body, high in safety, easy to store, suitable for batch production and wide in application prospect.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention discloses a biological fermentation feed for improving pork quality and a preparation method thereof, and belongs to the technical field of biological fermentation feeds. The biological fermentation feed comprises astragalus membranaceus powder, angelica sinensis powder, pericarpium citri reticulatae powder, codonopsis pilosula powder, liquorice powder, wild chrysanthemum flower powder, hawthorn fruit powder, rhizoma atractylodis powder, cinnamon powder, corn flour, soybean meal powder, bran powder, a bacterium enzyme complexing agent, compound enzyme liquid, compound chlorogenic acid liquid and drinking water. A preparation method of the compound enzyme liquid comprises the following steps: preparing a starch-tea polyphenol inclusion compound, and mixing; the biological fermentation feed prepared by the preparation method disclosed by the invention is not easy to decay when being placed in an open manner, is good in palatability, can greatly improve digestion and absorption of nutrient substances by pigs and improve the quality of pork, and does not need wall breaking treatment in the preparation process.

The application discloses a use of a pharmaceutical composition of a phenolic acid compound and probiotics, and particularly relates to a combination of chlorogenic acid and lactobacillus reuteri. The combination of chlorogenic acid and lactobacillus reuteri can improve glycolipid metabolic disorder, improve cognitive dysfunction, emotional disorder and delay aging, and can be used for preparing a medicine for treating metabolic disorder combined with neuropsychiatric diseases.

The invention relates to the technical field of organic chemical synthesis, and discloses a method for preparing a chlorogenic acid derivative by condensing chlorogenic acid and a bifunctional amino acid derivative, which comprises the following step: in the presence of EDCI and HOBt, reacting chlorogenic acid with the bifunctional amino acid derivative in a DMF solvent to obtain the chlorogenic acid derivative. The method does not need to protect hydroxyl, the synthesis steps are simple, and the reaction is green, environment-friendly and pollution-free.

The application discloses a dandelion medicinal material effective component rapid determination method based on color chroma values, and relates to the technical field of traditional Chinese medicinal material quality detection. * , a * , b * Chroma values of dandelion medicinal material powder are collected, and the contents of chicoric acid, monocaffeoyl tartaric acid and chlorogenic acid are determined by using high performance liquid chromatography as true values; subsequently, a neural network prediction model with chroma values as input and effective component contents as output is constructed and trained, preferably a single hidden layer structure; finally, the model is used for fast and nondestructive content prediction of unknown samples; compared with a traditional multiple linear regression model, the neural network model used in the application has significantly higher prediction accuracy, and the test set R² is all more than 0.86; the application realizes fast, nondestructive, low-cost and high-precision detection of dandelion medicinal material effective components, and is suitable for multiple scenes such as raw material quality control, market supervision, optimal harvest period judgment and fast pricing grading.

The application discloses a method for establishing a gynecological menstruation-regulating tablet fingerprint spectrum by using a high-performance liquid chromatography technology and application, by using the method, a good fingerprint spectrum is obtained, and the chromatographic peak is comprehensive, 12 fingerprint peaks are confirmed, wherein, corresponding attribution peaks of angelica sinensis, fried atractylodes macrocephala koidz, vinegar corydalis and chuanxiong are all in the gynecological menstruation-regulating tablet, and the angelica sinensis, fried atractylodes macrocephala koidz and vinegar corydalis all have exclusive peaks. By comparing with the reference substance, and combining with ultraviolet absorption spectrum characteristics, characteristic peaks of five chemical components, i.e., chlorogenic acid, ferulic acid, chuanxiongolide I, ligusticum lactone and butenyl phthalide are identified. The application further discloses a reference fingerprint spectrum of the gynecological menstruation-regulating tablet obtained by using the method, the gynecological menstruation-regulating tablet fingerprint spectrum detection method and the obtained reference fingerprint spectrum can objectively evaluate the overall quality of the gynecological menstruation-regulating tablet, and provide a research basis for quality control of the gynecological menstruation-regulating tablet.

This invention discloses a method for blanching and drying honeysuckle and its application, relating to the field of traditional Chinese medicine processing technology. The method includes: microwaving fresh honeysuckle with a power of 300-350 kW for 3-5 minutes; followed by drying the blanched honeysuckle at 80-90℃ for 3-5 hours. This invention, by employing a high-power microwave blanching combined with constant-temperature drying, significantly improves the retention rate of core active ingredients such as chlorogenic acid, flavonoids, and polyphenols in honeysuckle, while also achieving excellent sensory quality. Testing showed that the honeysuckle prepared by this method contained chlorogenic acid ≥140 mg / g, flavonoids ≥80 mg / g, and polyphenols ≥22 mg / g, with a weighted sensory evaluation score ≥90 points. The honeysuckle prepared by this method possesses excellent heat-clearing, detoxifying, antibacterial, anti-inflammatory, and antioxidant effects, making it suitable for continuous industrial production.

The invention discloses a method for improving the anti-oxidation characteristic of a soybean whey protein-dialdehyde starch composite film by using chlorogenic acid, and belongs to the technical field of food processing. The preparation method comprises the following steps: step 1, preparing soybean whey protein; step 2, preparation of dialdehyde starch; 3, preparing a film forming solution; and step 4, preparing the soybean whey protein-dialdehyde starch composite film added with chlorogenic acid. The chlorogenic acids with different concentrations are added into a soybean whey protein-dialdehyde starch solution to prepare the composite membrane, and the influence of the chlorogenic acids with different concentrations on the antioxidant property of the soybean whey protein-dialdehyde starch composite membrane is comprehensively evaluated by measuring the ABTS < + > and DPPH free radical scavenging rates of the composite membrane. And a theoretical basis is provided for improving the comprehensive performance of the edible film and promoting the application of novel food packaging. Comprehensive analysis shows that along with the increase of the concentration of the added chlorogenic acid, the anti-oxidation characteristic of the composite membrane is gradually enhanced, and the chlorogenic acid can effectively improve the anti-oxidation characteristic of the composite membrane.

The invention provides a broad-spectrum combined medicine for treating colorectal cancer. The broad-spectrum combined medicine is prepared from the following components in parts by weight: 100 parts of purified water, 38 to 90 parts of tripterygium wilfordii alkaloid, 38 to 90 parts of tripterygium hypoglaucum alkaloid, 28 to 85 parts of chamaejasmine, 28 to 85 parts of gelsmium elegans alkaloid and 28 to 85 parts of xanthoxylin. The medicine is prepared from the following raw materials in parts by weight: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata, 16 to 80 parts of astragaloside, 16 to 80 parts of oldenlandia diffusa alkaloid, 16 to 80 parts of sculellaria barbata alkaloid, 16 to 80 parts of tanshinone, 16 to 80 parts of carthamin, 16 to 80 parts of honeysuckle chlorogenic acid and 16 to 80 parts of pericarpium citri reticulatae flavone. By integrating anti-tumor components with multiple action mechanisms, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid and the like, tumor cell proliferation can be directly inhibited, and apoptosis can be induced; tanshinone, emodin and the like can inhibit tumor angiogenesis; ganoderma lucidum polysaccharides, astragaloside and the like can activate the immune system of the body, enhance the activity of T cells and NK cells, and form double strikes of direct killing and immune surveillance.

The invention relates to the technical field of apple production place detection, in particular to a red Fuji apple production place discrimination method based on organic component analysis, which comprises the following steps: S1, constructing a linear discrimination function of organic components such as oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin; s2, obtaining the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the apple sample to be detected; s3, inputting the contents of oxalic acid, succinic acid, tartaric acid, citric acid, malic acid, phlorizin, chlorogenic acid and rutin in the obtained apple sample to be detected into the linear discrimination function, and taking a group of maximum discrimination function calculation values as a production area detection result. According to the method, the production places of red Fuji apples in five production areas can be discriminated to a certain extent through linear discrimination established on the basis of eight organic components, the classification accuracy rate reaches 95.2%, and technical support is provided for the production place traceability of the Jinning apples and the quality control of the apples.

The invention provides a coffee and soybean milk composition for body weight management and a preparation method thereof. The coffee and soybean milk composition comprises the following components in parts by mass: 10-20 parts of a coffee component, 20-60 parts of a soybean protein component, 5-10 parts of a mitochondrial activator and 5-15 parts of dietary fibers. According to the coffee and soybean milk composition provided by the invention, the coffee component, the soybean protein component, the mitochondrial activator and the dietary fiber are scientifically and specifically proportioned, so that key functional components, namely caffeine and chlorogenic acid in coffee, generate a synergistic biological effect with the soybean protein; and a coordinated regulation system of a targeted lipid metabolism full chain is systematically constructed. According to the preparation method, the coffee-dietary fiber microcapsule powder is obtained through a microencapsulation process, the structure can effectively protect heat-sensitive components such as chlorogenic acid and part of aroma substances in coffee from being damaged in the processing and storage process, and the synergistic effect performance of beneficial components in the coffee and soybean milk composition in the human body is guaranteed.

The present application relates to a kind of bone repair compositions and its preparation method, belong to biological medicine technical field, pharmaceutical composition includes acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide, yak bone peptide, loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract;Acid anhydride bovine beta-lactoglobulin peptide is prepared using special enzymolysis method.Acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide and yak bone peptide are loaded using carboxymethyl chitosan-hydroxyapatite composite microspheres, then using sodium alginate-sodium carboxymethyl cellulose containing loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract one coating, then using sodium alginate-sodium carboxymethyl cellulose secondary coating.The present application develops high-efficiency pharmaceutical combination component, using polymer material to coat drug to form sustained-release system, improve bioavailability, using granular carrier to increase the stability and solubility of drug, promote intestinal absorption, improve bone repair process.

The invention discloses a chicoric acid carrier with a DNA regular tetrahedron as a skeleton and a preparation method of the chicoric acid carrier. According to the method, four oligonucleotide chains with specific sequences are synthesized, so that the oligonucleotide chains are self-assembled in a buffer solution to form a stable DNA regular tetrahedral structure. And then loading the natural active component chicoric acid with poor water solubility and low chemical stability into the interior or on the surface of the tetrahedron in a physical embedding or electrostatic adsorption manner. The obtained compound utilizes a hydrophilic skeleton of a DNA tetrahedron, so that the water solubility and chemical stability of the chicoric acid are remarkably improved, and the degradation rate of the chicoric acid under light, heat and oxidation conditions is effectively reduced. The problem that the clinical application of the chicoric acid is limited due to poor physicochemical properties, fast in-vivo metabolism and difficult targeted delivery is solved, and a new strategy is provided for further development of the chicoric acid.

The invention discloses a method for creating autotetraploid long-split opisthopapus taihangensis, and belongs to the technical field of medicinal plant breeding. The method comprises the following steps: soaking a diploid long-split opisthopapus taihangensis tender stem segment by colchicine, inducing to obtain an autotetraploid plant, and identifying by a flow cytometry and a chromosome counting method. The tetraploid opisthopapus longissima is remarkably superior to diploid opisthopapus longissima in drought tolerance and salt tolerance, and the content of chlorogenic acid and linarin in leaves is remarkably increased. The invention provides a new way for creation and efficient utilization of germplasm resources of the opisthopapus longissima, and has remarkable breeding and application values.