912 results about "Chlorogenic acid" patented technology

The invention provides temperature and pH responsive hydrogel entrapped with chlorogenic acid lipidosome as well as a preparation method and application of the temperature and pH responsive hydrogel, and relates to the technical field of biology. The temperature and pH responsive hydrogel entrapping chlorogenic acid lipidosome contains chlorogenic acid lipidosome, phenylboronic acid grafted modified gelatin and dopamine grafted oxidized hyaluronic acid, the concentration of the phenylboronic acid grafted modified gelatin is 3-10 wt%, the concentration of the dopamine grafted oxidized hyaluronic acid is 3-10 wt%, and the concentration of chlorogenic acid is 1-20 [mu] M. The temperature and pH responsive hydrogel can effectively promote wound healing independently or in combination with blue light.

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention discloses a bamboo powder-containing biodegradable plastic for food contact and a preparation method thereof, and relates to the technical field of bio-based materials. The bamboo powder-containing biodegradable plastic for food contact comprises chitosan starch and modified bamboo powder, the chitosan starch is prepared by adding composite chitosan and amino-terminated polyethylene glycol into oxidized corn starch, and the composite chitosan is prepared by grafting chlorogenic acid on the surface of chitosan and then coating succinic anhydride derivatives. The succinic anhydride derivative is prepared from dodecenyl succinic anhydride and 1, 4-butanediamine through a reaction, so that the plastic has the antibacterial property, and meanwhile, the mechanical property is enhanced; the modified bamboo powder is prepared by reacting bamboo powder with a carboxyl-terminated composition, and the carboxyl-terminated composition comprises carboxyl-terminated polylactic acid and carboxyl-terminated polyamide. The toughness of the plastic is enhanced, and the mechanical property of the plastic is further enhanced.

The invention provides a Chinese herbal medicine preparation for preventing and controlling African swine fever and a preparation method thereof. The Chinese herbal medicine preparation for preventing and controlling African swine fever provided by the invention comprises the following components in parts by weight: 4 parts of honeysuckle, 4 parts of fructus forsythiae, 5 parts of radix isatidis, 2 parts of astragalus membranaceus, 1 part of rhodiola rosea, 3 parts of anise, 0.5 part of roasted ephedra, 1 part of fried almond, 2 parts of gypsum, 2 parts of houttuynia cordata, 0.5 part of rheum officinale, 2 parts of rhizoma dryopteris crassirhizomae, 1 part of pogostemon cablin, 0.5 part of mint, 1 part of lalang grass rhizome, 1 part of dendrobe, 0.5 part of coptis chinensis and 0.5 part of liquorice. The Chinese herbal medicine preparation for preventing and controlling the African swine fever, provided by the invention, contains chlorogenic acid, luteoloside, calycosin-7-glucoside, forsythin, (R, S)-epigoitrin and aspimaric acid. And aspidin. And mongolic acid and the like. The composition has the functions of resisting viruses, resisting bacteria, improving immunity and the like. According to the Chinese herbal medicine preparation for preventing and controlling the African swine fever and the preparation method, provided by the invention, the African swine fever virus infection can be resisted, and the effect of promoting the rehabilitation of African swine fever virus recessive infected pigs is achieved.

The invention provides a mulberry leaf extract as well as a preparation method and application thereof in cosmetics, and relates to the technical field of fermentation and extraction of mulberry leaves. The preparation method of the mulberry leaf extract comprises the following steps: S1, inoculating a culture medium with compound bacteria to obtain a culture solution; s2, adding a mulberry leaf sample into the culture solution for fermentation, and feeding supplementary materials in the fermentation process to obtain fermentation liquor; s3, sterilizing, filtering, centrifuging and concentrating the fermentation liquid to obtain a crude extract; and S4, carrying out aqueous two-phase extraction, extraction, ultrafiltration and drying on the crude extract to obtain the mulberry leaf extract. The mulberry leaf extract prepared by the method has high contents of flavone, chlorogenic acid and peptide; the effects of inhibiting tyrosinase and removing DPPH free radicals are good.

The invention discloses a quality detection method for carpesium abrotanoides formula granules, and belongs to the technical field of traditional Chinese medicine detection. The detection method comprises the following steps: testing the total content of the specific chromatogram, neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid, and testing the content of carabrone. The method for testing the specific chromatogram and the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid comprises the following steps: a, preparing a neochlorogenic acid reference substance solution, a chlorogenic acid reference substance solution and a cryptochlorogenic acid reference substance solution; b, preparing a test solution; and c, carrying out liquid chromatography detection, and calculating the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid. The carabrone content test comprises the following steps: A, preparing a carabrone reference substance solution; b, preparing a test solution; and C, carrying out liquid chromatography detection, and calculating the carabrone content. The quality of the carpesium abrotanoides formula granules is effectively controlled, and the medication effectiveness and safety of the carpesium abrotanoides formula granules are guaranteed.

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention relates to the field of medicine, in particular to application of chlorogenic acid or pharmaceutically acceptable salt thereof in preventing, inhibiting or treating aortic dissection. The obvious effect of chlorogenic acid in treating the aortic dissection is found for the first time, and a brand new thought and method are provided for treating the aortic dissection. Besides, compared with the traditional surgical operation and interventional therapy, the chlorogenic acid is relatively low in treatment cost, so that the economic burden of a patient is relieved, and meanwhile, the consumption of medical resources is also reduced.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention relates to screening and application of a Candida antarctica lipase B mutant. Specifically, the Candida antarctica lipase B mutant provided by the invention is obtained by carrying out rational design and directed evolution combined strategy mutation on the basis of a wild Candida antarctica lipase B gene sequence, and the amino acid sequence of the mutant is shown as SEQ ID NO.3. The invention also relates to a preparation method of the Candida antarctica lipase B mutant. The mutant has higher enzyme activity and thermal stability, the hydrolytic catalytic activity of the mutant is improved to 2.1 times of the original hydrolytic catalytic activity, and the half-life period of the mutant enzyme at 50 DEG C is prolonged by 4 times. On the basis, pichia pastoris is used as a host to express the mutant, and a strain with hydrolytic activity improved by 52% is obtained through high resistance screening. The lipase is prepared into an immobilized enzyme to be used in a synthetic reaction of chlorogenic acid derivatives, the highest conversion rate of chlorogenic acid reaches 74% in 24 hours, and the immobilized enzyme has a good industrial application prospect.

The invention belongs to the field of preparation of sedation maintaining agents, and particularly relates to a sedation maintaining agent administrated through oral mucosa and a preparation method of the sedation maintaining agent. Bamboo leaf polysaccharide and pigskin collagen peptide are heated and mixed with chlorogenic acid and butyl acetate under the catalytic action of lanthanum nitrate, and the obtained moisturizing adhesive has biocompatibility, can be stably adhered to oral mucosa and can promote salivary secretion of children; nanoparticles obtained through freeze spray drying are uniformly dispersed in a copolymer solution containing sodium salicylate and polyacrylate, and the obtained nervous system regulator can balance excitability of sympathetic nerves and parasympathetic nerves of children; the emotion relieving composition contains modified felwort powder obtained through esterification reaction, the modified felwort powder is mixed with a moisturizing adhesive and a nervous system regulator, and the prepared sedation maintaining medicament administrated through the oral mucosa protects oral health of children in the oral operation anesthesia process, is free of irritation, improves anxiety emotion and does not affect postoperative awakening.

The invention relates to the technical field of abdominal wall adhesion materials induced by prevention and treatment of bacterial infection, and discloses an ionic liquid-based antibacterial-anti-inflammatory integrated bioactive nanoparticle with microwave sensitivity, a preparation method and application of the ionic liquid-based antibacterial-anti-inflammatory integrated bioactive nanoparticle. Then, the surfaces of the hollow nano particles are modified with bacteria specific targeting molecule 3-aminophenylboronic acid, and PBA-HPDA is formed; finally, chlorogenic acid and quaternary ammonium salt are combined according to a certain proportion to form ionic liquid (ILs) with a microwave heat effect, the ionic liquid (ILs) is wrapped in HPDA, and composite nanoparticles PBA-HPDA-CAQAC are formed. The nano particle material prepared by the invention has microwave heat effect and antibacterial and anti-inflammatory integrated biological activity, and has excellent bactericidal and anti-inflammatory effects.

The invention relates to the technical field of grapes, in particular to application of chlorogenic acid to improvement of grape fruit quality, and the dosage of the chlorogenic acid is 50-200M in mass concentration. The use method comprises the following steps: preparing the chlorogenic acid into a chlorogenic acid aqueous solution, and spraying the chlorogenic acid aqueous solution in a grape fruit swelling period or a grape fruit swelling later period once every 7-12 days for 3-6 times. By applying chlorogenic acid, the anthocyanin content of the grape fruits is remarkably increased, and the coloring effect of the grape fruits can be remarkably improved. By applying the chlorogenic acid externally, the fruit weight, the soluble solid content, the soluble total sugar content and the sugar-acid ratio of the grape fruits are increased, the titratable acid content is reduced, and the flavor and the nutrient content of the grape fruits are improved. The invention promotes the application and development of the chlorogenic acid in the maturation and quality of the grape fruits before harvesting, and solves the problems that the fruit ripening agent in the current market contains hormones and is high in price, the grapes are poor in coloring and slow in color turning, and the potential toxicity exists.

The invention belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV (at) HP based on hyaluronic acid as well as a preparation method and application of the temperature-sensitive hydrogel SV (at) HP. The temperature-sensitive hydrogel SV (at) HP entraps CS-siRNA nanoparticles and VEGF (at) PLGA microspheres, and is used for realizing microenvironment responsive drug delivery to treat intrauterine adhesion. Chlorogenic acid and TGF beta1 siRNA are self-assembled through disulfide bonds to form CS-siRNA nano-particles, the CS-siRNA nano-particles are used for treating intrauterine adhesion through gene and chemical therapy, and cell and animal experiments prove that the CS-siRNA nano-particles have a good effect of improving intrauterine adhesion. Through evaluation of a real-time fluorescence quantification method, a western blot method, a cell scratch experiment, laser confocal microscope observation, inverted fluorescence microscope observation, an in-vivo experiment and the like, the SV-coated HP gel has the effects of resisting inflammation, improving endometrial fibrosis, promoting IUA rat endometrial angiogenesis and repair, inhibiting intrauterine adhesion and improving the intrauterine environment.

According to the antibacterial and antioxidant composite film applied to food preservation and the production method of the antibacterial and antioxidant composite film, the chlorogenic acid, the catechin, the montmorillonite and the zeolite are introduced, and a drying process is optimized, so that the antibacterial performance, the antioxidant performance and the mechanical performance of the preservative film are remarkably improved, and meanwhile, the water vapor permeability and the oxygen permeability are reduced. The method comprises the specific steps of raw material preparation, polyphenol compound and mineral substance preparation, membrane liquid preparation and treatment, drying process optimization and finished product membrane uncovering. According to the preparation method disclosed by the invention, components such as chlorogenic acid, catechin, montmorillonite and zeolite are introduced, and a drying process is optimized, so that the food preservative film with synergistic antibacterial and antioxidant properties is prepared.

The invention relates to the technical field of biological materials, in particular to silver nano-chlorogenic acid composite hydrogel as well as preparation and application thereof. According to the invention, the CGA and the silver nitrate are used for green synthesis of the nano-particles CGA-AgNPs, and the nano-particles have peroxide nano-enzyme activity and antibacterial activity; and the CGA-AgNPs and chitosan (CS) are cross-linked to form the silver nano-chlorogenic acid composite hydrogel CGA-AgNPs-CS, so that the uniform distribution of nano-particles in the hydrogel can be promoted. According to the invention, multi-functionalization is taken as a target, a novel hydrogel composition system is designed from the source, the provided preparation method is green, simple, rapid and environment-friendly, the obtained composite hydrogel has multiple functions, and a good foundation is laid for expansion and application of the composite hydrogel in the biomedical fields of wound repair, wearable equipment, nano antibacterial drugs and the like.

The invention discloses a mucosal epithelial cell targeted oral ROS response type nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The oral ROS response type nano-enzyme comprises Ce-CDs carbon dots and a betaine polymer, and the betaine polymer is coated on the surfaces of the Ce-CDs carbon dots to form nano-particles; the Ce-CCDs carbon dots are prepared by taking a metal cerium source and chlorogenic acid as raw materials through a pyrolysis method. Through structural innovation and function integration, the prepared nano-enzyme shows outstanding advantages in the aspects of catalytic activity, targeted delivery, collaborative treatment, industrial application and the like, a new technical scheme is provided for efficient and safe treatment of inflammatory bowel diseases, and the nano-enzyme has remarkable technical innovation and clinical application value.

The invention provides a sarcandra glabra and honeysuckle compound preparation and a preparation method thereof, and during preparation, part of sarcandra glabra and honeysuckle are subjected to supercritical carbon dioxide extraction, volatile oil and medicine residues are collected, and the medicine residues and the rest of sarcandra glabra and honeysuckle are subjected to water extraction. A proper amount of beta-cyclodextrin is added into a water extract for inclusion, then concentration and supercritical extraction are performed to obtain volatile oil, and beta-cyclodextrin is used for inclusion, so that on the basis of increasing the efficacy of the volatile oil, the contents of chlorogenic acid and isofraxidin are increased, the curative effect of the medicine is enhanced, the synergistic effect of all components of the medicine is exerted, and the treatment effect of the medicine is improved. Menthol is clathrated with beta-cyclodextrin and then added into the paste powder to be mixed, granulated and tableted. On the premise of not influencing the dissolution of menthol and the direct stimulation effect on the throat, the effects of dispelling wind, clearing heat, relieving sore throat and relieving pain of the medicine are further enhanced, and meanwhile the problems that the quality of the product is not stable enough due to environmental change during storage, menthol is volatilized and is easy to crystallize, separate out and deteriorate are effectively solved.

The invention provides an environment-friendly PUR hot melt adhesive with excellent temperature resistance as well as a preparation method and application thereof, and belongs to the technical field of adhesives. The environment-friendly PUR hot melt adhesive is prepared from the following raw materials in parts by mass: 50 to 65 parts of polyester polyol, 10 to 20 parts of polyether polyol, 15 to 30 parts of isocyanate, 5 to 10 parts of modified isocyanate, 5 to 10 parts of chain extender and 3 to 5 parts of anti-aging additive, wherein the polyester polyol is formed by carrying out dehydration polycondensation on polybasic acid and polyol through a vacuum melting method, and the polybasic acid comprises binary acid, cinnamic acid, chlorogenic acid and tannic acid according to the mass ratio of (3-5): (0.5-0.8): (1.2-1.6): 1; the anti-aging auxiliary agent is a nano inorganic oxide containing a maleimide group; the modified isocyanate is a mixture obtained by reacting polyisocyanate, sulfamic acid, hydroxycarboxylic acid and tertiary amine. The PUR hot melt adhesive prepared by the invention has excellent thermal stability, and the cohesiveness of the PUR hot melt adhesive can be kept basically unchanged in a long-term high-temperature environment and a thermal cycle environment.

The invention discloses a metal polyphenol nano material based on chlorogenic acid and a preparation method and application thereof.The preparation method comprises the steps that chlorogenic acid and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 are mixed and dissolved in an anhydrous tetrahydrofuran solvent, and a first mixed solution is prepared; adding a FeCl3 solution into the first mixed solution until a clear and transparent system is formed, so as to obtain a second mixed solution; and removing the anhydrous tetrahydrofuran solvent of the second mixed solution to obtain the metal polyphenol nano material (CA-Fe NPs) based on chlorogenic acid. The metal polyphenol nano material has excellent photothermal conversion performance, and can be applied to in-vitro antibiosis, antioxidation, anti-inflammation and wound healing promotion.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses a method for efficiently preparing chlorogenic acid by using a stevia rebaudiana extracting solution, which comprises the following steps: concentrating the stevia rebaudiana extracting solution, and adjusting the pH value of the concentrated solution to 5.0-6.5 to obtain a to-be-treated solution; pumping the to-be-treated liquid into a simulated moving bed chromatographic system for separation to obtain a chlorogenic acid phase and a stevioside phase; adjusting the pH value of the chlorogenic acid phase to 3.0-4.5, then sequentially carrying out nanofiltration membrane concentration and thermal concentration, and finally carrying out spray drying to obtain a chlorogenic acid product. According to the method, ethanol extraction, simulated moving bed separation and nanofiltration membrane desalination are combined, the yield of the prepared chlorogenic acid is high, the extraction efficiency is high, the technological process is simple, and the production cost is low. In addition, stevioside is also recycled in the process of preparing the chlorogenic acid.

The composition is of a network structure and is composed of strontium ions and chlorogenic acid, and the weight ratio of the strontium ions to the chlorogenic acid is 1: (9-11). The composition and hydrogel are mixed to prepare a microneedle patch, and the microneedle patch is prepared into a medical instrument for wound repair. Through verification, the SrC-MPNs is continuously released by the SrC-MPNs (at) MN-PP, so that the substances are dissolved and absorbed into tissue fluid around a wound, and strontium ions and chlorogenic acid in the SrC-MPN can stably and continuously exert the effects of resisting bacteria and oxidation, promoting angiogenesis, promoting wound healing and the like. An in-vivo experiment result shows that the SrC-MPNs (at) MN-PP can promote in-vivo and in-vitro wound healing, promote M2 macrophage polarization, remove excessive ROS and promote angiogenesis and tissue regeneration so as to promote healing of diabetic wounds.

The present invention relates to the field of plant extraction, and in particular to an extraction process for skin care active ingredients in coffee peels. The present invention adopts a natural and pollution-free coffee peel extraction method, and extracts the natural active chemical components in the coffee peels through enzymatic biomimetic extraction, which can achieve the simultaneous extraction of chlorogenic acid, caffeic acid, and resveratrol in the coffee peels. The obtained extraction product has a high content of skin care active ingredients, which can be used as a cosmetic raw material to exert anti-inflammatory, anti-oxidation, whitening, soothing and other skin care effects; this technology makes full use of the production waste of coffee peels, reduces environmental pollution, recycles resources, and improves economic benefits.

The invention discloses a phenolic acid inhibitor and a use method and application thereof.The phenolic acid inhibitor comprises a component A. The component A comprises phenolic acid, polyethylene glycol and a phosphate buffer solution, and the phenolic acid is one or more of cumaric acid, kojic acid, chlorogenic acid, gallic acid and ferulic acid; the phenolic acid inhibitor is prepared from the following raw materials in parts by weight: 1 to 3 parts of phenolic acid, 0.1 to 0.3 part of polyethylene glycol and 8 to 12 parts of phosphate buffer solution, and the phenolic acid is two or three of coumaric acid, chlorogenic acid and ferulic acid. The PPO inhibition activity of the phenolic acid inhibitor can reach 70%-80%, the quality of fruits is well maintained, and browning caused by fruit processing is delayed.

The invention provides a method for detecting medicinal components of a traditional Chinese medicine composition UPLC-MS / MS. The detection method can be used for identifying 14 medicinal components of the traditional Chinese medicine composition, namely saikoside A, saikoside D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, narcissoside, kaempferitrin, lobetyolin, amygdalin, hyperoside and rutin. And a reference is provided for evaluating the internal quality of the traditional Chinese medicine composition.

A preparation method of a polyester nanofiltration membrane with high chlorine resistance and pollution resistance relates to the technical field of chlorine-resistant polyester nanofiltration membranes, and comprises the following steps: sequentially dipping a polyacrylonitrile support layer in an aqueous phase solution and an oil phase solution, carrying out condensation polymerization at 25-45 DEG C to generate a polyester selection layer on the surface, and finally heating and crosslinking at 45-65 DEG C to obtain the polyester nanofiltration membrane. The aqueous phase solution is a tannic acid aqueous solution, a rhamnose aqueous solution, a vitamin C aqueous solution or a chlorogenic acid aqueous solution, the preparation method is simple and low in cost, and the formed polyester nanofiltration membrane has high chlorine resistance and pollution resistance.

The invention discloses a morchella esculenta extract as well as an extraction method and application thereof, and belongs to the technical field of plant medicine product separation. The invention relates to a specific extraction method of phenolic acid of morchella, through the synergistic effect of low-temperature plasma pretreatment, targeted enzymolysis and deep eutectic solvent extraction, the extraction rate of phenolic acid is remarkably improved, particularly, specific extraction of chlorogenic acid can be realized, and the purity of chlorogenic acid is greater than 85%. The process is environment-friendly and efficient, and the obtained product has important application value in the fields of inflammation resistance and oxidation resistance.

The invention discloses a plant cell activating factor as well as a preparation method and application thereof. The plant cell activating factor contains active ingredients such as resveratrol, quercetin, chlorogenic acid and the like. The active ingredients of the plant cell activating factor are obtained from natural plants, so that the plant cell activating factor has no pollution to the environment, is safe and harmless to human bodies and animals, and meets the requirements of green agricultural development. Experiments prove that the plant cell activating factor disclosed by the invention can effectively regulate the physiological activity of plant cells, activate the growth potential of plants, promote the development of plant root systems, thick and strong stalks and dark green leaves, and remarkably improve the growth speed and biomass accumulation of the plants, thereby increasing the yield of crops.

The invention discloses a composition for promoting wound healing of pets and a preparation method and application of the composition. A nutritional supplement comprises chlorogenic acid and glutamine. The nutritional supplement is wide in raw material source, healthy, rich in nutrition, good in palatability, low in price and easy to obtain; by using the specific formula, especially the chlorogenic acid and glutamine in a specific ratio, nutrient substances required by growth and development of pets can be supplemented, postoperative wound healing of the pets can be effectively promoted, postoperative pain, discomfort and inflammation of the pets can be reduced, immunity of the pets can be improved, no antibiotic component is added into the supplement, and the supplement is free of toxic and side effects. The liver and kidney burden of pets can be relieved, and the risk that bacteria generate drug resistance is avoided; the preparation method of the nutritional supplement only needs processing technologies such as crushing, sieving, homogenizing and emulsifying, the operation process is simple and easy to learn, and industrial large-scale production is facilitated.