37 results about "Oleanolic acid" patented technology

The invention discloses a Lactobacillus paracasei CCFM1455 strain, a polysaccharide prepared from the Lactobacillus paracasei CCFM1455 strain for promoting gastric mucosa repair and application of the Lactobacillus paracasei CCFM1455 strain in synergism with rhizoma polygonati, and belongs to the technical field of microorganisms and medicines. According to the lactobacillus paracasei CCFM1455 exopolysaccharide disclosed by the invention, by regulating an STAT3 / SOCS3 signal channel, the levels of gastric mucosal lesion markers PGE2 and TFF3 are remarkably reduced, the expression of inflammatory factors is regulated, and gastric barrier protection and inflammation relief are realized. In addition, when the strain is used for fermenting polygonatum sibiricum, double synergistic effects can be generated: (1) macromolecular substances such as polygonatum sibiricum saponin are biologically converted, and easily absorbed micromolecular active substances such as oleanolic acid and liukucoumarin are generated; (2) polygonatum polysaccharide is converted into high-activity compound polysaccharide, so that the mucous membrane repairing synergistic effect is enhanced; therefore, the lactobacillus paracasei CCFM1455 and the fermentation derivative thereof provide an innovative solution for targeted intervention of gastric mucosal lesion and modernization of traditional Chinese medicines.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

To provide a conjugate for transferring an effector molecule from outside a cell into said cell.SOLUTION: Provided is a conjugate comprising an effector molecule, a cell surface molecule targeting antibody, and a saponin. Therein: the effector molecule, the antibody, and the saponin are covalently bound to each other; the effector molecule is an oligonucleotide; the saponin is a bi-desmosidic triterpene glycoside comprising an aglycone core structure selected from quillaic acid or gypsogenin and comprising a first glycan bound to a C3 atom of the aglycone core structure and a second glycan bound to a C28 atom of the aglycone core structure; the first glycan is branched and comprising a glucuronic acid unit; the second glycan is branched and comprising a carbohydrate unit selected from any one or more of fucose (Fuc), rhamnose (Rha), and quinovose (Qui); and the antibody is capable of binding to a cell surface molecule of the cell.SELECTED DRAWING: None

To provide wound-healing formulations containing solid birch bark extracts that may be applied to the skin without worsening the patient's condition.SOLUTION: A solid birch bark extract comprises at least about 70 wt.% of betulin, and one or more triterpenes selected from the group consisting of betulinic acid, oleanolic acid, erythrodiol, and lupeol.SELECTED DRAWING: None

Disclosed herein are oral care compositions, and preferably, oral care compositions providing reduced gingival bleeding, as well as methods for producing such oral care compositions. According to some aspects, there is provided a substrate having a stannous source; a nitrate source; a source of phosphate radicals; a surfactant system, the surfactant system comprising a sulfate based anionic surfactant; oleanolic acid; and eugenol, and an oral care composition.

The invention provides an external composition for promoting surgical incision healing and a preparation method thereof, and belongs to the technical field of medicines. Comprising a component A and a component B. The component A is powder and is prepared from banyan bark powder, traditional Chinese medicine powder, a decellularized material, collagen peptide and modified nano-hydroxyapatite according to the mass ratio of (2-4): (7-10): (3-5): (1-3): (2-4). And the component B is liquid and consists of carboxylated ionic liquid, chlorogenic acid / oleanolic acid-loaded chitosan, platelet-rich plasma and water in a mass ratio of (3-5): (5-8): (10-12): (200-300). The external composition for promoting surgical incision healing has good antibacterial, antioxidant and anti-inflammatory effects, can relieve pain, promote tissue regeneration, stop bleeding and restore, is safe and not prone to sensitization, achieves sustained and controlled release drug release, promotes surgical incision healing and scar repair, and has wide application prospects.

The invention discloses a strongly-bundled high-insulation glass fiber cloth and a preparation method thereof, and relates to the technical field of glass fiber cloth preparation, the glass fiber cloth is obtained by dipping a fiber cloth obtained by crushing, melting, drawing, yarn preparation, sizing and weaving of a glass raw material in a surface treating agent, after the yarn is subjected to sizing treatment, the strong bundling property meets the requirement, and the surface treating agent is obtained by mixing waterborne polyurethane emulsion prepared from functional diisocyanate and the like with a reinforcing agent and the like. The functional diisocyanate is obtained by carrying out a reaction on 1, 3-dihydroxyethyl adamantane, 3-amino-5-nitrobenzoic acid methyl ester, 4-amino-3-methyl benzonitrile, oleanolic acid and the like; the reinforcing agent is obtained by loading a product e containing aza-adamantane, phosphamide, cyano, hydroxyl and castor oil-based alkane long chains on halloysite nanotubes. The glass fiber cloth disclosed by the invention has relatively high insulativity, mechanical strength, high temperature resistance and flame retardance.

The invention belongs to the technical field of medicines, and particularly relates to an experimental method for treating liver cancer by using oleanolic acid. Comprising an in-vitro experiment method and an in-vivo experiment method. The in-vitro experiment method comprises the following steps: S1, cell culture; s2, cell proliferation and migration; s3, detecting cell apoptosis and a cell cycle; s4, performing immunoblotting; s5, carrying out real-time quantitative PCR; the in-vivo experiment method comprises the following steps: step 1, performing chick embryo chorioallantoic model experiment; and 2, carrying out tumor-bearing experiment, treatment and tissue sampling on the mouse. The application of the oleanolic acid in liver cancer treatment is systematically researched for the first time, it is found that the oleanolic acid has the remarkable effects of inhibiting proliferation, inducing apoptosis, resisting angiogenesis and the like on liver tumors through the synergistic effect of multiple mechanisms, the remarkable curative effect on cancer mice is achieved, and a new medicine choice is provided for liver cancer treatment.

The application belongs to the field of new adjuvants and new dosage forms of pharmaceutical preparations, and particularly relates to a type I and type II immunogenic cell death inducer ion pairing engineered nanoassemblies as well as construction and application thereof. The nanoassemblies are composed of type I ICD inducers, type II ICD inducers, hydrophobic auxiliary counterions and amphiphilic lipid ion pairing. The type I ICD inducer is selected from mitoxantrone, doxorubicin, oxaliplatin or cyclophosphamide; the type II ICD inducer is selected from hypericin; and the hydrophobic auxiliary counterion is selected from cholesteryl sodium sulfate, cholic acid, deoxycholic acid, chenodeoxycholic acid, lithocholic acid, glycolcholic acid, taurocholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, deoxycholic acid lysine derivative, oleanolic acid and ursolic acid. The type I and type II ICD inducers are combined in the application, and have a synergistic tumor treatment effect.

The invention relates to the technical field of liqueur, in particular to liqueur and a preparation method thereof.The liqueur is prepared by soaking various medicinal and edible raw materials such as kudzuvine roots, rice, barbary wolfberry fruits, smoked plums, grapes, mulberries, poria cocos, Chinese yams, rhizoma polygonati, jujubes and ginseng in baijiu base liquor according to a specific proportion. According to the present invention, the modern analysis technology shows that the wine contains 25 active components such as resveratrol, protocatechuic acid, oleanolic acid, quercetin, kaempferol and the like, and the components achieve the synergistic effect, such that the product has effects of tumor resistance, liver protection, blood sugar reduction, blood fat reduction, oxidation resistance, inflammation resistance, nerve protection and other health-care functions, and the product has characteristics of definite component, synergistic effect, high safety, and no toxic-side effect. The preparation process is stable and controllable, and is suitable for large-scale production.

The invention provides a 5 alpha-reductase inhibitor. The inhibitor is pawpaw total triterpenes (TCS). The invention also provides application of the pawpaw total triterpenes TCS in preparation of drugs for treating benign prostatic hyperplasia. TCS and UA have no obvious toxic and side effects on visceral organs of each group of rats; the thickness of glandular epithelial cells in a TCS group is remarkably reduced, the 5AR activity of the TCS group is remarkably reduced, the T level is remarkably reduced, and the DTH level is remarkably reduced; the blood sugar of the TCS group is obviously reduced, the concentration of total cholesterol and low-density lipoprotein is obviously reduced, and the TCS can obviously increase the high-density lipoprotein level and reduce the content of triglyceride. The results show that the TCS and the main component tartaric acid have a remarkable inhibiting effect on prostatic hyperplasia. The inhibition of 5AR activity and the reduction of DHT generation and T content are one of the action mechanisms, and the important characteristic is that the prostatic hyperplasia can be inhibited and abnormal blood sugar and blood fat can be regulated at the same time.

The invention belongs to the field of plant genetic engineering, and particularly relates to an entada phaseoloides EpCYP88 gene and application of the entada phaseoloides EpCYP88 gene to production of entada phaseoloides acid by catalyzing oleanolic The invention discloses a key gene EpCYP88 in biosynthesis of entada phaseoloides specific metabolite entada phaseoloides acid. The nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the EpCYP88 protein coded by the gene is as shown in SEQ ID NO. 2; the protein is used as a key enzyme for catalyzing the dihydroxylation of oleanolic acid to realize the heterologous production of entada phaseoloides acid in a model plant (tobacco). Conditions are created for mass production of entada phaseoloides acid in tobacco, and the cost of obtaining entada phaseoloides acid is greatly reduced.

The invention provides a chlorhematin-containing plant growth regulator as well as a preparation method and application thereof, and relates to the technical field of plant growth regulators. The plant growth regulator containing chlorhematin is prepared from the following raw materials in percentage by mass: 0.2 to 0.5 percent of microencapsulated chlorhematin, 3 to 6 percent of triterpenoid saponin, 1 to 2 percent of oleanolic acid-beta-cyclodextrin inclusion compound, 3 to 6 percent of tea saponin, 2 to 4 percent of sodium lignin sulfonate, 0.2 to 0.5 percent of polyether modified organic silicon defoaming agent, 1 to 3 percent of sodium polyacrylate dispersing agent, 0.5 to 1.5 percent of polyvinyl alcohol and the balance of water. 0.5-1.5% of sodium carboxymethyl cellulose and the balance of pre-gelatinized starch; the microencapsulated hemin wall material consists of sodium alginate, chitosan and gelatin in a mass ratio of 3: (2-3): 1; the triterpenoid saponin is gynostemma pentaphyllum saponin and / or ginsenoside.

The invention belongs to the technical field of medicine detection, and particularly relates to a detection method of a gastrodia elata Taifengling capsule, the gastrodia elata Taifengling capsule is prepared from gastrodia elata, achyranthes bidentata, radix scrophulariae, rehmannia, angelica sinensis, eucommia ulmoides, ligusticum wallichii, mistletoe, wild chrysanthemum flower and uncaria, and the detection method comprises the following steps: carrying out microscopic identification on the gastrodia elata by adopting a microscopic identification method; carrying out thin-layer chromatography identification on radix scrophulariae, ligusticum wallichii, angelica sinensis and oleanolic acid by adopting a thin-layer chromatography method; and determining the content of gastrodin in the gastrodia elata medicinal material and the content of linarin in the wild chrysanthemum medicinal material by adopting a high performance liquid chromatography. According to the detection method disclosed by the invention, a quality control system of the gastrodia elata naolingling capsule is established, the adopted detection method is scientific and reasonable, high in accuracy and good in reproducibility, the quality of the gastrodia elata naolingling capsule can be comprehensively and effectively controlled, and the clinical curative effect of the product is ensured.

The application discloses a detection method and application of ursolic acid and total triterpenes in prunella vulgaris seed and prunella vulgaris seed oil. The detection method is characterized in that ursolic acid in prunella vulgaris seed and prunella vulgaris seed oil is qualitatively identified by using a thin layer chromatography method; oil and fat components interfering with determination of total triterpenes are separated and removed by column separation, and then the content of total triterpenes in prunella vulgaris seed and prunella vulgaris seed oil is determined by using spectrophotometry with ursolic acid or oleanolic acid as a control, so that the problems of interference and false high content in determination of the content of total triterpenes in oil components in the prior art are overcome. The method has the advantages of high accuracy and precision, good stability and repeatability, and provides a method for quality detection of triterpenes in development and utilization of prunella vulgaris seed and prunella vulgaris seed oil, and has strong specificity and good reproducibility.

The invention discloses a kind of oleanolic acid and ursolic acid identification method in gynecological antipruritic tablets, and relates to the technical field of Chinese medicine quality control. Oleanolic acid and ursolic acid identification method of the present invention include the preparation of test solution, the preparation of reference solution, thin layer plate spotting, developing agent development, spraying display agent color development and the step of inspection, also including in situ pretreatment;The in situ pretreatment is:After the thin layer plate spotting ends, one end of the thin layer plate spotting is immersed in prepared iodine ‑ dichloromethane solution, after soaking spotting point 1.0 ‑ 1.5cm, it is quickly taken out, and covered with a glass plate, the glass plate is removed after being placed for 30 ‑ 50min, the solution remaining on the thin layer plate is waved away, and it is observed that brown on the thin layer plate fades, that is, to obtain final product. The present invention is applicable to the simultaneous identification of oleanolic acid and ursolic acid in Hedyotis diffusa.

The application discloses a thin-layer identification method of Belamcanda chinensis in gynecological itching-relieving tablets and relates to the technical field of traditional Chinese medicine quality control. The thin-layer identification method is characterized in that ursolic acid and oleanolic acid are used as the control samples, the developing agent is composed of ethyl ether and dichloromethane in a volume ratio of 0-2:6-9, and the color developing agent is 10% sulfuric acid ethanol solution.

The invention relates to eutecticevaporate gel and a preparation method and application thereof, and belongs to the technical field of gel materials, the eutecticevaporate gel is obtained by photo-initiation polymerization of a polymeric monomer, an eutecticevaporate solvent and a cross-linking agent; the polymeric monomer is acrylamide; the eutectic solvent is prepared from cyclodextrin and organic acid through a reaction; the mass ratio of the cyclodextrin to the organic acid is 1: (2-6); the organic acid is at least one of phytic acid and oleanolic acid; the cross-linking agent is methacrylated gelatin. The eutectic gel has mechanical properties matched with human skin, good biocompatibility and strong adhesion, can respond to metal matrix protease so as to be quickly degraded, and can meet different use requirements in the field of controlled release of medical drugs.

The invention relates to a composition for oral or topical human administration and a preparation method thereof. The present invention relates to pentacyclic triterpenoid compounds wherein said triterpenoid compounds are incorporated into lipid vesicles, preferably cream globules, surrounded by three layers of membranes. The triterpenoid is preferably betulinic acid, and / or betulinol, and / or lupenol, and / or oleanolic acid, and / or ursolic acid, most preferably betulinic acid. The incorporation of the triterpenoid compound into the milk fat globules or lipid vesicles at a high payload of at least 10 to at most 50 mg / ml is preferably accomplished with high energy emulsification techniques, most preferably ultrasound treatment. The composition is present as a liquid emulsion formulation in a preferred embodiment. In another preferred embodiment, the composition is a powder, which can be prepared from a liquid embodiment of the composition by, for example, spray drying or freeze drying techniques. The composition can be preferably used as a medicament, a food supplement or a nutraceuticals for the treatment or prevention of cancer, type 2 diabetes, obesity and cardiovascular diseases.

An oleanane-type triterpenoid or a glycoside thereof is useful as a compound having an effect of inducing formation of cilium and activating hair papilla cells or hair matrix cells.

The present application relates to the field of cosmetics, especially to an anti-aging essence and a preparation device, which is composed of A phase, B phase, C phase and D phase, and the components of each phase are as follows according to weight percentage: A phase: deionized water 16.15%, rose hydrolat 18.00%, propylene glycol 5.00%, glycerol 4.00%, xanthan gum 0.15%, carbomer 940 0.10%, panthenol (vitamin B5) 2.00%, boswelia (30% aqueous solution) 10.00%, ikedoin 2.00%, nicotinamide 2.00%, sodium hyaluronate compound 0.50%; B phase: polyglyceryl-6 distearate 1.20%, squalane 3.00%, isononyl isononanoate 2.00%, octyldodecanol 5.00%, rosehip oil 0.50%, argan oil 0.50%, ceramide compound powder 0.50%, cholesterol 0.30%, free fatty acid (C18-C22) 0.20%, Transcutol P 4.00%, oleanolic acid (ligustrum lucidum extract) 0.50%, plant extract compound liquid 5.00%, ergothioneine 0.30%. The advantages are that the active ingredient utilization rate is improved, the efficacy is comprehensive and the synergistic effect is strong, and the preparation device can make the mixing of each component more uniform, greatly improving the mixing efficiency.

The present invention proposes a kind of external-use composition promoting the healing of surgical incisions and its preparation method, belong to the field of medical technology.Including component A and component B, wherein component A is powder, and is composed of banyan bark powder, Chinese medicine powder, acellular material, collagen peptide, modified nano hydroxyapatite, and mass ratio is 2 4:7 10:3 5:1 3:2 4;Component B is liquid, and is composed of carboxylated ionic liquid, chlorogenic acid / oleanolic acid chitosan, platelet-rich plasma and water, and mass ratio is 3 5:5 8:10 12:200 300. The external-use composition promoting the healing of surgical incisions obtained by the present invention has good antibacterial, antioxidant and anti-inflammatory effects, analgesic and granulogenic, hemostasis recovery, and safety is not easily sensitized, realizes slow-controlled release drug release, promotes the healing of surgical incisions, the repair of scar, with broad application prospects.

This invention provides a CYP716 gene, protein, and applications for catalyzing the oxidation of β-amyrin at the C22α and C28 positions, belonging to the field of biotechnology. The CYP716 gene includes either CYP716BX1 (nucleotide sequence as shown in SEQ ID No. 1) or CYP716BX2 (nucleotide sequence as shown in SEQ ID No. 2). Studies have shown that CYP716BX1 and CYP716BX2 are isoenzymes, both capable of catalyzing the oxidation of β-amyrin at the C22α and C28 positions to 22α-hydroxy-kapogonadiol or 22α-hydroxy-oleanolic acid, and also capable of catalyzing the oxidation of 21β-hydroxy-β-amyrin at the C22α and C28 positions to 21β,22α-dihydroxy-kapogonadiol. This invention can provide key gene elements for the synthetic biology research of cyrimyl alcohol-type triterpenoids.

The invention discloses a dogwood flower extract with anti-oxidation and blood sugar and lipid reducing effects as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention aims to solve the technical problems of regulating blood sugar, insulin, blood fat and antioxidant level by activating a PI3K / AKT / mTOR pathway in vivo, improving liver, kidney and pancreas injuries and exerting anti-diabetic activity by reducing inflammatory factor expression. According to the key point of the technical scheme, the invention discloses a dogwood flower extract with the effects of resisting oxidation and reducing blood sugar and blood lipid. The active ingredients of the dogwood flower extract comprise morroniside, swertioside, loganin, ursolic acid, oleanolic acid, dogwood neoglycoside, gallic acid, protocatechuic acid, p-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, orientin, rutin, ferulic acid, benzoic acid, resveratrol, quercetin and cinnamic acid.

An oleanolic acid-producing industrial hemp endophyte fungus, HSDMZ07, and its applications relate to the field of microbiology. This fungus, belonging to the genus Fusarium, has been deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, on June 17, 2024, with a deposit number of CCTCC NO: M20241243. This fungus, HSDMZ07, can ferment to produce oleanolic acid and exhibits good antibacterial activity, showing some inhibitory effects against Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis. The present invention is used for both antibacterial and oleanolic acid production.

The invention relates to the field of antibacterial hydrogel, and discloses an oligopeptide molecule and application thereof, oleanolic acid hydrogel as well as a preparation method and application thereof. The structural formula of the oligopeptide molecule is shown in the formula (I). The oligopeptide molecule provided by the invention has an excellent application prospect in preparation of a hydrogel material. # imgabs0 #

The present invention provides a cytochrome P450 oxidase gene and application of Bupleurum chinense, which is CYP716A41. The enzyme has been confirmed by catalytic experiments to have the function of catalyzing the biosynthesis intermediate of saikosaponin β-amyrin and producing erythrodiol and oleanolic acid in its C-28 multi-step oxidation. The present invention has achieved the heterologous production of erythrodiol, an intermediate in the synthesis of saikosaponin, by using the enzyme, and erythrodiol is an important synthetic intermediate of various C-28 hydroxylated oleanane-type saikosaponins and other C-28 hydroxylated site triterpenoids. The present invention further promotes the analysis of the biosynthetic pathway of saikosaponins and synthetic biology research, and has important research value for the synthesis and regulation of oleanane-type pentacyclic triterpenoids such as saikosaponins and the breeding of Bupleurum plants.

The invention provides a conyza blinii extract (tablet) fingerprint spectrum detection method, which comprises: A) taking a test sample raw material, and pre-treating to obtain a liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; b) measuring the liquid to be measured by adopting a high performance liquid chromatography-evaporative light scattering method to obtain an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) fingerprint spectrum of the Conyza blinii extract (tablet); chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% formic acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography-evaporative light scattering method is adopted, an acetonitrile-0. 1% formic acid aqueous solution is selected as a mobile phase for gradient elution, ursolic acid, oleanolic acid, beta-amyrin, alpha-amyrin and friedelin are taken as reference substances, and the conyza blinii extract (tablet) fingerprint detection method is established. The scientificity and the internal quality of the conyza blinii extract (tablet) are integrally evaluated.

The invention relates to the technical field of functional food, in particular to a composition with the effects of reducing blood sugar and regulating blood fat and application of the composition. The invention provides a composition with the effects of reducing blood sugar and regulating blood fat, the composition comprises a cyclocarya paliurus triterpenoic acid compound and a selenoprotein additive, and the effect is optimal when the mass ratio of ursolic acid to oleanolic acid to betulinic acid to arjiurolic acid in the cyclocarya paliurus triterpenoic acid compound is optimized to be 1: 3.21: 3.37: 0.74 through a response surface experiment. According to the invention, the cyclocarya paliurus triterpene component and the selenium food nutrition enhancer are used at the same time, so that the cyclocarya paliurus selenium food nutrition enhancer can reduce blood sugar, regulate blood fat and increase the antioxidant capacity of the body, also has the functions of inhibiting postprandial blood sugar rise, relieving insulin resistance and protecting liver islet, and can meet the selenium intake of 50-200 micrograms per day of adults recommended by Chinese Nutrition Association; the health-care food can be used as a novel functional food to be applied to prevention and health care of sub-health people with hyperglycemia and hyperlipidemia.