107 results about "Clarkia" patented technology

The invention relates to a bottom-dwelling food organism medium for cultivating procambarus clarkia and a using method of the bottom-dwelling food organism medium; the medium and bottom sediment at bottom of a pool are mixed to be injected into river water filtered by a 60-mesh bolting-silk for cultivating aquatic bottom-dwelling organisms and proliferative waterweeds; the cultured food organism can replace more than 50% of artificial diet, the cultivation cost is greatly reduced, and the high-quality bottom-dwelling food organisms can provide a nutrient source for rapid growth of the procambarus clarkia, so as to greatly improve the growth size, yield and benefit; and the produced high-quality commodity shrimps can have improved market competitiveness, and reduced cultivation cost and cultivation risk.

The invention discloses a method for culturing a procambarus clarkia by reforming a paddy field into a pond. The method comprises the following technical steps: (1) reform of the paddy field; (2) construction of an escape-proof facility; (3) culture and propagation of a parent shrimp; (4) preparation of a pelletized feed; (5) formation of a larva; and (6) fishery. The method can be used for increasing the per-unit yield of the procambarus clarkia quickly and can also propagate a large quantity of young shrimps for personal culture and sale, thereby improving the culture yield and the culture benefit obviously.

The invention provides a procambarus clarkia deveining machine which comprises a shrimp tail conveying mechanism, a shrimp intestine removing mechanism, a main stand and a subordinate stand, wherein the shrimp tail conveying mechanism is fixed on the main stand; the shrimp intestine removing mechanism is fixed on the subordinate stand; the shrimp tail conveying mechanism is used for conveying and clamping shrimp tails; and the shrimp intestine removing mechanism is used for shearing tail fans and pulling out shrimp intestines. The deveining machine disclosed by the invention is skillful in structure, and since the shrimp tail conveying mechanism and the shrimp intestine removing mechanism are matched in use, the deveining machine disclosed by the invention can be used for removing the shrimp intestines without damaging the shrimp tails. The deveining machine is high in production efficiency and mechanical degree, and can be used for completely replacing manual labor to remove the shrimp intestines, saving expensive labor cost and greatly reducing the production cost of the shrimp tails; and the deveining machine disclosed by the invention is suitable for the large-scale processing of the procambarus clarkia tails.

The invention discloses a Chinese herbal medicine immunopotentiator for resisting white spot syndrome virus (WSSV) of procambarus clarkia, as well as a preparation method and an application thereof. The preparation method comprises the following steps: milling rheum officinale, epimedium, astragalus membranaceus and isatis root and fully mixing the ingredients in proportion to prepare the powdery immunopotentiator. The Chinese herbal medicine immunopotentiator disclosed by the invention is stable and wide in raw material resources, simple to prepare, and low in cost, is based on the immunomechanism of shellfish to provide Chinese herbal medicines for the shellfish, and can be used for improving the immunity and oxidation resistance of a body during application, improving the capacity of the procambarus clarkia for resisting WSSV infection, and promoting the growth of the procambarus clarkia.

The present invention discloses a feed for procambarus clarkia. The feed is prepared from the following components in parts by weight: 30-35 parts of corn flour, 25-30 parts of puffed soybean meal, 5-10 parts of distillers' grains, 2-3.5 parts of yeast powder, 20-25 parts of flour, 10-15 parts of spirulina platensis powder, 5-10 parts of green vegetable leaves, 2-3 parts of chicken intestine powder, 5-10 parts of earthworm powder, 2-3 parts of dried small ctenopharyngodon idellus powder, 5-10 parts of sunflower seed oil residues, 0.2-0.4 part of garlicin, 1-3 parts of Chinese yams, 2-3 parts of wild chrysanthemum, 0.5-1.2 parts of licorice, 2-4 parts of leonurus artemisia, 2-3 parts of honeysuckle, 1-1.5 parts of compound vitamins, and 1-1.5 parts of trace elements. Advantages are as follows: the feed contains various nutrients, is scientific in formula and comprehensive in nutrition, and satisfies basic nutrition of the procambarus clarkia. At the same time, the vitamins and trace elements are added, so that the feed ensures the procambarus clarkia to grow rapidly, improves immunity, and is free of toxic and side effects, and also harmful substance residues.

The invention discloses a procambarus-clarkii environment-friendly sinking puffing feed and a preparing method. The feed is prepared from protein source-fat source-sugar source raw materials, composite vitamins, composite mineral substances, antioxidants, phagostimulants and adhesives in a certain proportion. The preparing method includes the steps that A, the feed raw materials are respectively smashed and screened, the obtained mixture and the adhesives are mixed to be even, the fat source raw materials are added while stirring, water is adding while stirring after even mixing, and feed base materials are prepared; B, the feed base materials are input into a double-shaft extrusion puffing machine, a slicing motor is started, and the extrusion puffing machine operates in the modes that a switch is switched on, a main motor is started, the feed base materials are matured and extruded through a mold hole for extrusion puffing, semi-particle feed is obtained and matured, and the puffing particle feed is obtained. The formula is reasonable, use is convenient, the culturing cost and the incidence rate of procambarus clarkia are reduced, the disease resistance of procambarus clarkia is improved, and the sustainable development of the procambarus clarkia culturing industry is achieved.

The invention relates to procambarus clarkia compound feed, which belongs to shrimp feed. The invention is characterized in that the procambarus clarkia compound feed comprises the following ingredients in percentage by weight: 19 to 22 parts of soybean cake powder, 16 to 19 parts of full wheat powder, 7 to 9 parts of fish meal, 9 to 11 parts of colza cake powder, 9 to 11 parts of cottonseed cake powder, 8 to 10 parts of rice spermoderm, 8 to 10 parts of corn powder, 6 to 8 parts of animal harslet dry powder, 1.5 to 2.5 parts of fish oil, 1.5 to 2 parts of zeolite powder, 1 to 1.5 parts of ground caustic, 1.5 to 2.5 parts of composite mineral substance powder, 1 to 2 parts of premix compound (earthworm, beetle and beer yeast) dry powder, 1 to 1.5 parts of slough powder, 0.1 to 0.3 part of vitamin B complex, 0.05 to 0.15 part of common salt, 0.03 part of antioxidants and 0.05 part of mildew inhibitors. The trial use shows that the invention has the advantages that: 1. the procambarus clarkia compound feed has overall nutritive elements and reasonable mixture ratio, the growth speed of the fed procambarus clarkia is improved by 20 to 30 percent than that of the procambarus clarkia fed with existing randomly prepared feed; and 2. because the palatability is good, the digestive absorption utilization rate is high, and compared with that when feeding the existing randomly prepared feed, the practical culture production cost is reduced by 15 to 25 percent.

The invention discloses a C-type agglutinin gene of procambarus clarkia as well as a preparation method and applications thereof. The method comprises the following steps: A. extracting total RNA (ribose nucleic acid); B. synthetizing a first chain cDNA; C. acquiring partial nucleotide sequences; D. acquiring the nucleotide sequences at 3'-RACE: 3' end; E. acquiring the nucleotide sequences at 5'-RACE: 5' end; F. acquiring the complete nucleotide sequences of the gene; and G. coding an amino acid sequence of protein by the C-type agglutinin gene. From the cloning in procambarus clarkia to theC-type agglutinin gene, the gene is cloned to a PET-28a carrier, recombinant protein mClec is expressed in colon bacillus BL21 (DE3), and the purified mClec expresses agglutination effect to multiplebacteria and rabbit red blood cells; and the protein mClec or recombinant agglutinin obtained from cloning prokaryotic expression vector on the agglutinin gene can be used for producing shrimp and fish antivirus and antibacterial medicaments.

The invention relates to a quick-acting environment-friendly pool cleaning method for procambarus clarkia. The quick-acting environment-friendly pool cleaning method comprises the following steps that derris roots are cut into thin slices and are crushed into fine powder after being dried, the sieving is carried out by a bolting silk screen, and derris root powder is prepared; during the procambarus clarkia pool cleaning, about 1-1.5kg of derris root powder is used for 667m<3> of water bodies and is added into a pool to be soaked by clean water, then, the water and the slag are simultaneously and uniformly spread into the pool, and wild trash fishes can be fast killed in 2-4 hours; and after the pool cleaning and sterilization through the derris root powder, fermented organic manure of pigeon manure or rabbit manure can be spread into the water bodies, and shrimp seeds can be placed into the pool after 5-7 days after the fermentation. The natural Chinese herbal medicine plant of the derris is adopted to be prepared into the pool cleaning medicine, the process is simple, the operation is convenient, and compared with the conventional pool cleaning medicine, the prepared derris root powder has the advantages that the effect taking speed is high, the efficiency is high, the medicine dosage is low, the production cost is low, green and environment-friendly effects are realized, the degradation in the water bodies is easy, no medicine residue is left, the derris root powder is safe on pool remaining propagating parent procambarus clarkia, shrimp seeds and waterweeds, and the like. The method provided by the invention is particularly suitable for being used for pool cleaning during the procambarus clarkia culture.

The invention relates to a feed capable of improving immunity and yield of Procambrus clarkii and a production method thereof. The method is characterized in that: after being processed and smashed, bean pulp, fish meal, wheat middling, corns and adhesive are mixed with fish oil, phosphatide oil, monocalcium phosphate and multidimensional premix to be prepared into granulated feed; and embedded cellulase, breaking yeast powder and fermentation shrimp shell meal are added to be fully stirred to be prepared into the granulated feed. The invention can change parts of cellulose taken by the Procambrus clarkii into saccharide, thereby being favourable for lowering feed coefficients; shrimp shell powder can kill the pathogen in shrimp shells after being performed with high-temperature fermentation; meanwhile, the chitin in the shrimp shells is decomposed into chitosan and oligosaccharide to improve the immunity and the survival rate of the Procambrus clarkia; and the breaking yeast with obvious food calling effect is added to improve the utilization ratio of the feed, thereby being favourable for improving cultivation yield and benefits.

The invention discloses a procambarus clarkia feed additive as well as a preparation method and use thereof, belonging to the technical field of aquatic product feed additives. The procambarus clarkia feed additive comprises the following raw materials in parts by weight: 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata. The preparation method comprises the steps of weighing 1 part of honeysuckle, 0.8-1.2 parts of radix astragali, 0.6-1.0 part of poria cocos and 0.7-1.1 parts of houttuynia cordata according to parts by weight, respectively soaking, poaching and filtering the raw materials to obtain filtrates, respectively concentrating the filtrates to obtain honeysuckle concentrated solution, radix astragali concentrated solution, poria cocos concentrated solution and houttuynia cordata concentrated solution, mixing the concentrated solutions to obtain the procambarus clarkia feed additive. The procambarus clarkia feed additive has the advantage of remarkably improving the sum of blood corpuscles, the lysozyme, the superoxide dismutase, the acid phosphatase and the alkaline phosphatase of the procambarus clarkia to ensure the healthy growth and reduce the death rate by using the honeysuckle, the radix astragali, the poria cocos and the houttuynia cordata to form the additive.

The present invention discloses a compound Chinese herbal medicine preparation for preventing and curing white spot disease (WSD) of procambarus clarkia and a method thereof. The compound Chinese herbal medicine preparation comprises the following components: radix isatidis, rheum officinale, herba houttuyniae and allicin. The method comprises the following steps: the compound Chinese herbal medicinal preparation is mixed with feed and the feed is continuously used to feed for 30-40 days before a season of an onset of the WSD of the procambarus clarkia. Compared with the prior art, especially for ponds which the WSD outbreak, the provided compound Chinese herbal medicine preparation for preventing and curing the WSD of the procambarus clarkia can control the incidence of the WSD of the procambarus clarkia to a very low level, the method is simple in operations, and the preparation has very important meanings for preventing and curing the WSD of the procambarus clarkia, reducing morbidity of the procambarus clarkia caused by the WSD, and improving yields.

The present invention discloses a feed for procambarus clarkii and a preparation method thereof. The feed for procambarus clarkia comprises the following raw material components: flour, rice bran, rapeseed meal, peanut meal, soybean meal, super steam fish meal, cottonseed meal, corn gluten meal, bentonite, brewer's yeasts, spray-dried animal blood cells, squid cream, monocalcium phosphate, semen ricini kernels, phosphatide oil, fish oil, choline, a mildew preventive, shrimp trace elements, shrimp vitamins, vitamin C, an antioxidant, inositol and carnitine. The feed for the procambarus clarkii can inhibit the gonad development and inhibit natural ovulation in the development period of the procambarus clarkia, thereby increase the weight gain, and increase the economic benefits. The preparation method of the feed for the procambarus clarkii is simple and convenient in operation, and conductive to improving the breeding efficiency.

The present invention discloses a feed for procambarus clarkia. The feed comprises the following raw materials in parts by weight: 85-130 parts of fishmeal, 375-450 parts of soybean meal, 40-70 parts of fermented soybean meal, 90-120 parts of puffed soybeans, 220-260 parts of puffed wheat, 40-60 parts of rice bran, 22-28 parts of calcium dihydrogen phosphate, 4-7 parts of choline chloride, 8-12 parts of compound minerals, 2-5 parts of complex vitamins, 1-1.5 parts of a shelling agent, and 4-6 parts of edible salt. All the components in the feed recipe are natural substances, ensure basic nutrition of the feed for the procambarus clarkia, and at the same time increase effective ingredients of proteins, carbohydrates, phosphorus, calcium, vitamins, minerals, etc., so that the feed for the procambarus clarkia is comprehensive and balanced in nutritional ingredients, effectively enhances immunity, disease resistance, a variety of stress resistance ability of shrimps, strengthens growth of the shrimps, improves survival rate, and are comprehensive and balanced in the nutritional ingredients.

The invention relates to a method for preparing a seasoning by utilizing the zymolysis of the heads of procambarus ckarkii, which pertains to the technical field of aquatic product processing. The method takes the heads of procambarus ckarkii as a raw material and the seasoning product is acquired by removing impurities, concentration and drying after the completion of zymolysis and hydrolysis. The method utilizes the heads of procambarus ckarkii as the raw material, is beneficial to promoting the processing level of the wastes of agricultural products, livestock products and aquatic products of China and can produce great economic benefit.

The invention discloses a gene sequence of encoding an antibacterial peptide-chitin peptide cloned from procambarus clarkia, and provides a recombinant expression and purification method thereof. The invention further discloses an amino acid sequence of the antibacterial peptide-chitin peptide cloned from the procambarus clarkia. The invention further provides the uses of the gene sequence and the chitin peptide. In the invention, a yeast expression vector can be prepared by virtue of the procambarus clarkia chitin peptide gene of the invention; an engineering strain can be obtained by transfecting Pichia stipitis; and recombinant protein with antibacterial activity can be obtained by induction expression. The recombinant chitin peptide obtained by the invention can be applied to an antibacterial feed additive, food preservation, animal and plant gene transformation and drug development.

The invention discloses a preparation method for a procambarus clarkia simple sequence repeats (SSR) primer. The preparation method comprises the steps: (1), extracting procambarus clarkia genome DNAs: extracting the genome DNAs from procambarus clarkia abdominal muscles; (2), hybridizing a probe and a target segment: hybridizing the biotin labeled probe and the target segment to obtain a hybridization product; (3), enriching magnetic beads: enriching the hybridization product by a magnetic bead enrichment method to obtain an enrichment product; (4), performing positive clone sequencing, and designing a primer amplification gene DNA according to a microsatellite flanking sequence; (5), performing SSR primer detection. The preparation method for the procambarus clarkia SSR primer has the advantages that a microsatellite sequence of the procambarus clarkia whole genome can be simply and quickly screened; a large number of SSR primers can be developed at a short time; technical measures are provided for germ plasma conservation and genetic improvement of the procambarus clarkia.

The invention relates to a method for comprehensively utilizing procambarus clarkia by-products, which comprises the following steps of: preparing heads and shells of procambarus clarkias into shrimp powder at first; leaching and extracting the shrimp powder by using mixed acid prepared from citric acid solution and malic acid solution and distilled water; carrying out auxiliary extraction of leach liquor through ultrasonic wave, and drying filtered filtrate in vacuum to obtain citric acid-calcium malate; washing leached residues by using water to be neutral, and drying; carrying out enzymolysis of the leached residues by using compound protease prepared by mixing papain and flavourzyme and distilled water, deactivating enzyme after carrying out enzymolysis, centrifuging to obtain supernate and precipitate, and concentrating, freezing and drying supernate to obtain protein powder; and extracting and filtering the precipitate by using extractant prepared by mixing pure ethyl acetate and absolute ethyl alcohol, and concentrating, freezing and drying filtrate in vacuum to obtain astaxanthin powder. According to the invention, calcium, protein and astaxanthin in procambarus clarkia by-products are extracted step by step; the environmental pollution problem of shrimp enterprises is well solved; and resource saving is realized to the most extent.

The invention discloses a method of quickly analyzing group and number of blood lymphocytes of various common crustaceans according to the principles that with the particle structure in the blood lymphocytes of crustaceans, such as litopenaeus vannamei, procambarus clarkia, eriocheir sinensis and the like, as classification reference, the blood lymphocytes, in different groups, can be discriminated and statistic analysis is automatically completed at the same time with a region, where sample points are located, and auxiliary images as evidences by means of a multi-dimensional panorama analyzer having an image auxiliary function. The method is free of dyeing and avoids influence due to manual steps such as normal dyeing, observation under a microscope and the like; by means of automatic signal analysis of a high-sensitivity detector, the method has good repeatability. The multi-dimensional panorama analyzer has high-throughput detection performance, so that the reliability of a result from tens of thousands cell sample points is far higher than that of manual counting. The method is very low in usage amount of reagents and consumables and is low in cost due to trace amount detection. The method not only can be used for scientific analysis and daily monitoring, but also can supply technical support to classifying collection of the blood lymphocytes, so that the method has great application value.

The invention discloses a feeding attractant for juvenile procambarus clarkii as well as a preparation method and application of the feed attractant. The feeding attractant comprises the following components in parts by weight: 30-40 parts of lycine, 15-25 parts of kanirin, 15-20 parts of dimethyl-beta-propiothetin and 20-30 of allicin. The preparation method of the feeding attractant comprises the following steps: weighing the components according to the weight parts and then mixing uniformly. The raw materials of the feeding attractant is stable in source and can be taken from a wide range, the production process is simple, the cost is low, and the effect is obvious; from the food intake and digestion mechanism of crustacean, the feeding attractant improves the food intake rate of procambarus clarkia bodies and reduces the waste of baits, simultaneously, the activity of digestive enzymes of the intestines and pancreases of the juvenile procambarus clarkii is improved to promote the growth of the procambarus clarkii.

The invention discloses a preparation method of Chinese herbal medicine compound fermented feed for procambarus clarkia. The feed is prepared from a fermentation substrate, a bacterial agent and a Chinese herbal medicine compound extract by a multiple fermentation process; the mass ratio of the bacterial agent, the Chinese herbal medicine compound extract and the fermentation substrate is (0.2-5):(0.2-4):50000, and the fermentation substrate comprises, in parts by mass, 5-15 parts of soybean meal, 30-40 parts of wheat, 30-40 parts of corn, 10-15 parts of bran, 1-5 parts of molasses, 1-3 partsof compound premix and 10-20 parts of water; and the Chinese herbal medicine compound extract comprises, in parts by mass, 15-20 parts of yucca, 15-20 parts of cortex eucommiae, 15-20 parts of radix astragali, 10-15 parts of garlic, 10-15 parts of common fenugreek seeds and 10-15 parts of folium mori. The number, activity and survival time of intestinal probiotics in the product are guaranteed, the Chinese herbal medicine compound extract and the compound premix with specific composition have characteristics of micro ecological preparation and functions of antibiotic-like growth factors, the immunity and disease resistance of the procambarus clarkia can be significantly enhanced, the disease incidence can be decreased, and the feeding quality can be improved.

The invention discloses procambarus clarkia inhibin (prohibin 1) gene with a nucleotide sequence as shown in SEQ ID NO.1. The invention further provides inhibin coded by the crawfish inhibin 1 gene with the nucleotide sequence as shown in SEW ID NO.2. The invention further provides a use of the gene sequence and a use of the inhibin. The procambarus clarkia inhibin 1 disclosed by the invention can be used for preparing a prokaryotic expression vector, so that escherichia coli is converted and recombinant protein with antiviral activity is expressed. The recombined PHB (Polyhydroxybutyrate) obtained by utilizing the procambarus clarkia inhibin 1 gene can be used for antiviral feed additive, animal and plant gene transformation and medicinal development.

The invention relates to ecological feed for procambarus clarkii and a production method thereof. The feed comprises the following components in parts by weight: 3-5 parts of imported fish meal, 2-4 parts of meat and bone meal, 10-12 parts of puffed soybeans, 8-10 parts of fermented soybean meal, 6-8 parts of peanut meal, 4-6 parts of rapeseed meal, 8-10 parts of shrimp powder, 1-2 parts of squidpaste, 3-5 parts of scallop skirt powder, 1.0-2.0 parts of fish oil, 1-2 parts of soybean oil, 1-2 parts of soybean lecithin, 1-2 parts of corn oil, 0.2-0.4 part of cholesterol, 20-22 parts of flour,6-8 parts of corn flour, 5-7 parts of rice bran, 2-3 parts of algae powder, 0.2-0.5 part of choline, 0.5-1.0 part of multivitamins, 1.5-2 parts of calcium lactate, 0.5-1.0 part of composite mineral salt, 0.3-0.5 part of vitamin C phosphate and 0.1-0.2 part of gamma-aminobutyric acid. The protein level and the phosphorus content of the feed are reduced, the feed digesting and absorbing rate of theprocambarus clarkia is increased, and the concentrations of the total nitrogen and the total phosphorus in procambarus clarkia aquaculture water are significantly reduced.

The invention relates to an indoor soilless procambarus clarkii culture method and an artificial cave for the same. The method comprises steps as follows: a multi-layer stereoscopic box rack is arranged indoors, and a plurality of aquaculture water tanks are placed on each layer of stereoscopic box rack; plastic aquatic plants and the artificial cave are arranged in each tank in proportion according to size of the tank, a long strip-shaped corridor is reserved between the artificial cave and the aquatic plants after the artificial cave and the aquatic plants are placed, baits are mainly thrownin the corridor, a few short and small passages are reserved in other places, and a small part of food is thrown to the passages; adult procambarus clarkia are cultured at the density of 30-40 / m<2>,or juvenile procambarus clarkii are cultured at the density of 400-800 / m<2>, or procambarus clarkii with larvae are cultured at the density of 2-4 / m<2>; the baits are thrown once in the morning and evening every day. With the adoption of the culture method with controllable temperature, controllable illumination, controllable water level and controllable feeding, the demand of normal molting of procambarus clarkii for hidden things can be met by the artificial cave and with the method of plastic aquatic plant placement.

The invention relates to a special feed containing shell-broken yeast powder for procambrus clarkia and a production method thereof, which is characterized by mixing and producing soybean meal, fish meal, wheat middling, corn and bond after being smashed with fish oil, phospholipids oil, calcium biphosphate and multi-dimension pre-mixed material, and the shell-broken yeast powder is added to be adequately stirred the uniformly-mixed materials are cured by steam inside a conditioner and is squeezed by a granulating machine to granulated feed, and the granulated fed is packed to obtain the finished product after being dried and cooled. The shell-broken yeast powder with remarkable food calling effect and high nutrition value is added, so the utilization rate of the feed can be improved, the production cost can be reduced, and the culture yield and the culture benefit can be increased; and moreover, the special feed has the advantages of less waste, fast growth of the shrimp postlarvae, high culture yield and good economic benefit after feeding the procambrus clarkia.

The invention discovers procambarus clarkia anti-lipopolysaccharide factor gALF1 gene for the first time and the nucleotide sequence of the gALF1 gene is shown as SEQ ID NO.1. According to the invention, a gALF1 protein is prepared by utilizing the gene coding, and the amino acid sequence of the gALF1 protein is shown as SEQ ID NO.2. The invention also discloses application of the gene and the protein in the field of immunization and treatment of WSSV virus infection. The procambarus clarkia anti-lipopolysaccharide factor gALF1 gene disclosed by the invention has an effective antiviral effectas well as extremely strong inhibiting and killing effects on the WSSV virus; and immune and treatment problems of WSSV virus infection can be solved effectively.

Provided is an ecological pond breeding method of procambarus clarkia. The ecological breeding method comprises the following steps of 1, pond preparation; 2, pond cleaning and disinfection; 3, pond maintenance before breeding; 4, pond water injection and aquatic plant planting; 5, shrimp seed stocking; 6, bait feeding; 7, water quality control; and 8, timely fishing. The ecological pond breedingmethod of the procambarus clarkia can overcome the correlative defects that the daily management of existing crayfish is not good, the water quality is polluted easily to cause high death rate, poor quality, low yield of the crayfish.