54 results about "Specific growth" patented technology

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The invention relates to the technical field of aquaculture, in particular to an accurate regulation and control method for micropterus salmoides culture water quality. According to the micropterus salmoides culture water quality regulation and control method, a culture water body meets one or more of the following conditions: (1) adding photosynthetic bacteria into the culture water body every day, and controlling the final concentration of the photosynthetic bacteria in the water body to be 10 < 6 >-10 < 7 > CFU / mL; (2) adjusting the content of dissolved oxygen in the culture water body to 5.5-7.5 mg / L; (3) adjusting the temperature of the culture water to 26 + / -1 DEG C in the daytime and 23 + / -1 DEG C at night. The total nitrogen, total organic carbon and chemical oxygen demand can be obviously reduced through day and night temperature regulation and addition of photosynthetic bacteria, the water change demand is reduced, and the eutrophication risk is reduced; meanwhile, the specific growth rate (SGR) and the weight growth rate (WGH) are improved, the immune and antioxidant indexes are optimized, and the aeration energy consumption is reduced by controlling dissolved oxygen to be 7.0 mg / L.

This invention discloses an irrigation resource allocation optimization system based on environmental perception, relating to the field of agricultural irrigation technology. It includes: an environmental data acquisition module for real-time acquisition of soil moisture, air humidity, precipitation, light intensity, ambient temperature, and wind speed within the irrigation area, using the acquired data as a real-time environmental parameter set; and a crop growth stage determination module for determining the specific growth stage of the crop based on a preset crop growth cycle model and the real-time environmental parameter set. This invention uses the environmental data acquisition module to obtain multi-dimensional environmental parameters such as soil moisture and air humidity in the irrigation area in real time. The crop growth stage determination module determines the specific growth stage of the crop based on the preset crop growth cycle model and the real-time parameters. The baseline water requirement acquisition module then matches the baseline water requirement for the corresponding stage from a database, solving the problem in existing technologies that fail to connect the dynamic water requirements of the crop growth period with real-time environmental parameter fluctuations.

The invention belongs to the technical field of composite strains, particularly relates to a high-lignin straw fermentation composite microbial agent, and a preparation method and a fermentation process thereof, and aims to solve the problems of low lignin degradation efficiency, long period and high cost of a single microorganism in the prior art. According to the invention, a composite synergistic system of coriolus versicolor, bacillus subtilis and pichia pastoris is innovatively adopted, and efficient degradation is realized through a mechanism that the coriolus versicolor secretes a lignin degrading enzyme system, the bacillus subtilis assists cellulose decomposition and the pichia pastoris optimizes a microenvironment; the preparation method of the complex microbial inoculant comprises the following steps: culturing various strains in a proper culture medium to a specific growth state, and mixing according to an optimized volume ratio; the fermentation process relates to high-lignin straw pretreatment, inoculation, sealed fermentation under control conditions and regular ventilation, the lignin degradation rate can be increased, the feed nutritional value can be improved, the relative feeding value and quality can be improved, and economic and environment-friendly technical support is provided for resource utilization of agricultural by-products.

The invention relates to a stem cell technology, in particular to a pet stem cell induction culture medium, a preparation method and a culture method, and the culture medium significantly improves the proliferation efficiency and multidirectional differentiation potential of adipose-derived stem cells by optimizing the proportion of specific growth factors. The preparation method comprises the steps of basic culture medium preparation, growth factor addition and sterile filtration. Experimental verification shows that compared with a control group, the adipogenic differentiation efficiency of ADSCs cultured by the culture medium is improved by 40%, the osteogenic differentiation ALP activity is improved by 65%, and the positive rates of surface markers CD29, CD44 and CD90 are all greater than or equal to 95%. The problems of animal-derived pathogen pollution risk and low induction efficiency of a traditional culture medium are solved, and the culture medium is suitable for pet medical treatment.

The invention provides a prediction method of a cell culture cooling point and application thereof, and relates to the technical field of fermentation culture.The method comprises the steps that cell density data are obtained, the current specific growth rate mu current of cells is calculated after smoothing algorithm processing, and when mu current is smaller than or equal to a mu threshold value, it is judged that the cooling point is reached; the mu threshold value is equal to k * mu max; wherein the mu max is the maximum specific growth rate of the cells, the k is 0.4-0.6, and the mu max and the mu current are from the same batch of cells. According to the method, the specific growth rate mu of the cells is used as a credible process control decision variable for the first time, the hysteresis of a traditional living cell density method is overcome through robust data processing, prospective capture of the physiological state of the cells is achieved, and the product yield and the process robustness are remarkably improved.

The invention provides a method, a system, equipment and a medium for constructing a yeast high-temperature tolerance prediction model, and belongs to the field of microbial breeding, and the method comprises the following steps: determining a tolerance label based on a maximum specific growth rate, an ethanol yield and a final ethanol concentration, and obtaining transcriptome data and metabolome data of a saccharomyces cerevisiae strain, the metabolome data comprises the synthesis rate of the trehalose, and the synthesis rate of the trehalose is determined according to the net synthesis amount of the trehalose in the heat shock time of the saccharomyces cerevisiae strain; constructing feature vectors according to the transcriptome data and the metabolome data; and inputting the feature vectors into the model to determine a predicted tolerance score, and performing hyper-parameter optimization on the model according to the tolerance tag and the tolerance score of the saccharomyces cerevisiae strain in the training set to obtain a yeast high-temperature tolerance prediction model. According to the method, the built-in powerful nonlinear learning ability of the model is utilized, the complex mapping relation between the multi-omics characteristics and the high-temperature tolerance is mined, and the accuracy and reliability during model prediction are guaranteed.

The invention discloses an application of acetyl ursolic acid in promoting the growth of burkholderia. Eight Burkholderia strains are separated from soil, the separated eight Burkholderia strains are cultured in a nitrogen-free culture medium added with 50 mg / L of acetyl ursolic acid, and the result shows that the biomass of Burkholderia can be increased by 40%-95% compared with that of a control group without acetyl ursolic acid within 24 h through acetyl ursolic acid, the Burkholderia strain has a remarkable growth promoting effect on Burkholderia, and the Burkholderia strain has a good application prospect. The core functions such as nitrogen fixation are not influenced. The concentration gradient experiment further determines that the effective concentration range of the acetyl ursolic acid on the growth promotion of the burkholderia is 5-100 mg / L, and the optimal concentration of the acetyl ursolic acid is 25-75 mg / L. Acetyl ursolic acid is used as a specific growth promoting agent of burkholderia, not only are the problems of specificity, safety and stability in the prior art solved, but also the synergistic effect of promoting growth and improving crop quality is realized.

This invention relates to the field of microbial technology, and particularly to a peanut-specific growth regulator and its application in sugarcane-peanut intercropping. The peanut-specific growth regulator of this invention consists of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-6 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L-0.4 g / L of brassinolide, and 0.2 g / L-0.4 g / L of jasmonic acid. At this concentration, the peanut-specific growth regulator can significantly increase peanut yield. Under sugarcane-peanut intercropping conditions, the yield increase is even more significant. This peanut-specific growth regulator can provide a new approach for the development of specialized agricultural biological agents.

A growth prediction device includes a stage predictor configured or programmed to predict a growth stage for a crop, and a definer configured or programmed to define, when a period from a reference date to an accomplishment date of the growth stage includes a specific growth stage, a threshold value that indicates a prediction date for the specific growth stage with respect to transition data for the growth stage predicted by the stage predictor. When predicting a next growth stage, the stage predictor is configured or programmed to predict a date on which transition data for the next growth stage reaches the threshold value to be the specific growth stage.

The invention relates to the technical field of plant germplasm resource evaluation, and discloses a bamboo reed germplasm resource environmental adaptability evaluation method. The method comprises the following steps: acquiring environment data of a plurality of ecological test points and bamboo reed phenotype data, and forming a germplasm environment response record after matching; according to the bamboo reed key phenological period division points, the records are intercepted and recombined into a plurality of phenological response sub-records; for each sub-record, extracting an environmental factor extreme value exceeding a preset tolerance range and a duration, and constructing an environmental pressure portrait; connecting all portraits of the same bamboo reed germplasm in series at all test points and arranged according to a phenological sequence to form a cross-regional whole-growth-period environmental adaptability path diagram; and inputting the path diagram into a pre-trained multi-dimensional adaptability discrimination network for analysis, and outputting an adaptability grade classification label to finish evaluation. According to the method, environmental stress in a specific growth stage can be quantified more finely, the overall adaptability of germplasm to a complex stress sequence is systematically judged, and the accuracy and practicability of evaluation are improved.

The invention discloses a directional domestication method of anti-pollution algae species of haematococcus, and relates to the field of microbial breeding, key domestication indexes comprise specific growth rate, photochemical quantum yield, astaxanthin content and intracellular pollutant concentration; inputting the domestication key indexes into a resistance comprehensive scoring model, and outputting a resistance comprehensive score; and the pollutant concentration adjustment algorithm comprehensively scores according to the resistance of the current domestication period. According to the method, the resistance comprehensive scoring model is constructed, so that traditional qualitative resistance evaluation is upgraded to quantitative scoring. Scoring can objectively and comprehensively reflect the comprehensive resistance of the algal population, the problem of bifamily algal species caused by single index screening is avoided, and it is ensured that the finally obtained algal species have the anti-pollution capacity and the economic value at the same time. Whether the resistance comprehensive score reaches a target value or not is taken as a feedback signal, the pollutant concentration of the next period is automatically adjusted, the pressure is properly increased when the algal population is excellent in performance, and the pressure is timely reduced when the algal population is poor in performance. Compared with a traditional fixed gradient mode, the dynamic regulation and control mode can shorten the domestication period.

The present application relates to the technical field of plant germplasm resource evaluation, and discloses a reed germplasm resource environmental adaptability evaluation method. The method comprises the following steps: obtaining environmental data and reed phenotype data of multiple ecological test points, and matching to form a germplasm environmental response record; dividing the record according to a key reed phenophase, and recombining the record into multiple phenophase response sub-records; for each sub-record, extracting extreme values and duration of environmental factors that exceed a preset tolerance range, and constructing an environmental stress image; connecting all the images of the same reed germplasm at all test points in the order of phenophases to form a cross-regional whole growth period environmental adaptability path diagram; inputting the path diagram into a pre-trained multi-dimensional adaptability discrimination network for analysis, and outputting an adaptability grade classification label to complete the evaluation. The present application can more finely quantify environmental stress at a specific growth stage, and systematically discriminate the overall adaptability of germplasm to a complex adversity sequence, thereby improving the accuracy and practicality of the evaluation.

This invention discloses a silty substrate vegetation concrete for tung tree seed propagation and its application method. The silty substrate vegetation concrete is made by mixing silty soil, cement, seawater, and fertilizer. By pouring this mixture into seedling tubes and planting tung tree seeds, an integrated concrete seedling tube structure is formed. This tube can be directly planted in outdoor wetlands for tung tree seed propagation, thus achieving seed germination, seedling propagation, and planting. This invention utilizes silty substrate vegetation concrete to construct an integrated concrete seedling tube structure, which has suitable strength, porosity, and density, improving the specific growth rate and survival rate of tung tree seedlings and greatly simplifying the tung tree seed cultivation process.

The utility model discloses a fish ecological breeding device which comprises a breeding pond, supporting columns are fixedly connected to the inner surface of the breeding pond, rotating motors are fixedly connected to the interiors of the supporting columns, rotating columns are fixedly connected to the output ends of the rotating motors, rotating rods are fixedly connected to the upper surfaces of the rotating columns, and the rotating rods are fixedly connected to the lower surfaces of the rotating columns. A mounting groove is formed in the lower surface of the rotating rod, positioning columns are fixedly connected to the left side and the right side of the inner surface of the mounting groove, rolling wheels are rotatably connected to the outer surfaces of the positioning columns, baffles are fixedly connected to the lower surface of the rotating rod, the number of the baffles is five, and screening holes are formed in the four baffles. By screening the fishes with different body types, the fishes with the same body type can be intensively bred, so that a more accurate breeding strategy can be formulated according to the specific growth stages and body type characteristics of the fishes, such as reasonably adjusting the feeding amount, optimizing the breeding density and the like, and differentiated management on the fishes at different growth stages is facilitated.

This invention provides a culture medium for closed-system culture of mesenchymal stem cells, comprising the following components: 1% (v / v) human platelet lysate, 10-15 g / L basal culture medium powder, 15-20 mM HEPES, 1.0-2.0 g / L sodium bicarbonate, and 0.1-1.0 g / L sodium chloride; wherein the basal culture medium powder includes non-essential amino acids, D-glucose, L-glutamine, specific growth factors, inorganic salts, and vitamins. Using the culture medium provided by this invention for mesenchymal stem cell culture eliminates the need for exogenous CO2, reducing equipment costs and process complexity; furthermore, it results in cell viability ≥97%, more concentrated particle size distribution, and more stable expression of surface markers, which is beneficial for large-scale production.

This invention discloses a recombinant protein pPIC9K-17, its preparation method, and its applications, relating to the field of gene recombination technology. The recombinant protein pPIC9K-17 provided by this invention does not strictly follow the (Gly-X-Y)n triple helix repeat pattern of traditional collagen in its core structural unit. The repeating unit sequence includes a "GAIQ" sequence and a sequence containing collagen characteristics, possessing a certain rigid structural basis and containing receptor-binding or signal activation domains for specific growth factors. The direct tandem connection of multiple such units theoretically enables multivalent binding, enhancing affinity and signal activation efficiency with cell surface receptors, thus surpassing the biological functional limitations of single components (pure collagen or pure growth factors) or simple repeating structures. The recombinant protein of this invention not only effectively promotes the proliferation and migration of skin fibroblasts and accelerates collagen synthesis, but also shows clear efficacy in promoting hair regeneration (hair growth), and can be used to prepare products for skin repair and / or promoting hair regeneration.

The present invention relates to bacterial probiotics useful in aquaculture. Specifically, it discloses a selection of non-hemolytic, proteolytic, stress-tolerant bacterial strains with enhanced quorum- quenching capabilities. Moreover, the method allows detection of novel species of bacteria whose probiotic attributes could be massive for aquaculture. From the isolated bacteria, selected species were tested for their probiotic efficacy in Nile tilapia (Oreochromis niloticus), a key fish species in global finfish aquaculture. The species demonstrated significant efficacy in improving the health and growth performance of Nile tilapia juveniles. Specific growth rates and weight gain, and improved feed conversion ratios without adverse effects on fish health were observed. The gut microbiome was positively influenced by promoting beneficial bacteria while reducing harmful (opportunistic) species, leading to improved immune modulation. Survival rates were significantly boosted during a pathogenic challenge, and key immune genes were upregulated, indicating stronger local immune defenses. These findings show that the selected probiotic species offer substantial benefits for growth, feed efficiency, and disease resistance, emphasizing their potential for widespread application as probiotics in aquaculture.

The invention provides an application of bacillus amyloliquefaciens HS17 in water quality regulation and control and vibrio inhibition, the bacillus amyloliquefaciens HS17 provided by the invention is used as a single strain, can simultaneously realize the synergistic functions of inhibiting specific vibrio pathogenic bacteria, efficiently degrading multiple nitrogen sources and constructing stable biological floccules, does not need to depend on multi-strain mixing, and has the advantages of simple preparation process, low cost and high efficiency. Bacterial strain screening, compounding and application operations in aquaculture are greatly simplified, and the production and use cost is reduced; in addition, when a microbial preparation containing the strain is applied to culture of penaeus vannamei boone and tilapia mossambica, the survival rate and the specific growth rate of cultured animals can be remarkably increased; and the bacillus amyloliquefaciens HS17 shows no hemolytic activity, has no toxic risk to bred animals and ecological environment, and has high safety.

The invention belongs to the technical field of biology, and provides bullfrog protein peptide with antioxidant activity and a preparation method and application thereof, and the method comprises the following steps: decoloring pretreated bullfrog leftovers to obtain decolored leftovers; after degreasing treatment, stirring at normal temperature and washing to obtain crude protein; adding a reaction medium and protease for enzymolysis, separating soluble peptide after inactivation, and freeze-drying to obtain solid protein peptide. The bullfrog whole leftovers are subjected to decoloration, degreasing and enzymolysis for preparation, the method is simple and efficient, protein resource utilization is improved, and industrial application is facilitated; when the additive is applied to a perch feed, the oxidation resistance and the growth performance of perch can be improved, when 1% of the additive is added, the CAT activity and the SOD activity of perch are improved by 40.72% and 51.94% respectively compared with those of a control group, when 0.5% of the additive is added, the weight gain rate and the specific growth rate are improved by 11.46% and 4.41% respectively, and the additive has a good application prospect.

The invention provides application of ergothioneine in improving growth of large yellow croakers in a wintering period, improving liver oxidation resistance and enriching intestinal flora, and belongs to the technical field of aquaculture. According to the method, 25 mg / kg and 50 mg / kg of ergothioneine are added into feed for the larimichthys crocea juvenile fish in the wintering period for feeding, and results show that the ergothioneine can remarkably improve the weight gain rate, the specific growth rate and the muscle and whole fish crude fat content of the larimichthys crocea juvenile fish; the activity of CAT, T-SOD and GSH-Px in the liver of a test group is remarkably improved, and the content of MDA is remarkably reduced; in addition, the ergothioneine can improve the intestinal villus height of the larimichthys crocea juvenile fish in the wintering period and improve the abundance of intestinal flora, the abundance of harmful bacteria is reduced, and the abundance of beneficial bacteria is increased. The invention proves that the ergothioneine can promote the growth of the larimichthys crocea juvenile fish in the wintering period, enhance the oxidation resistance and improve the intestinal health, and can be used as a green feed additive for the larimichthys crocea in the wintering period.

The invention provides a special agricultural product planting intelligent control system based on a microcontroller and an operation method, and the system comprises a plurality of sensors which are used for collecting the growth period recognition information and various environmental data of special agricultural products; the dual-mode protocol conversion gateway is used for distributing communication time slots for a plurality of sensors and receiving data acquired by the sensors through an RS485 communication protocol and a LoRa communication protocol respectively; the MCU is used for generating a plurality of control instructions according to the growth period identification information, the various environmental data and the user input information; and the relay is used for adjusting the running state of the corresponding external equipment according to the plurality of control instructions. According to the system and the control method, deep coupling of crop physiological characteristics and Internet of Things resource scheduling is realized, the system deployment and maintenance difficulty is reduced, and meanwhile, the accuracy of regulation and control decision making of specific growth stages or spatial differences of characteristic agricultural products is improved.

The application discloses application of Prunella vulgaris extract in prevention and treatment of fish parasitic disease infection. Inclined tube worm in-vitro soaking experiments show that the insecticidal effect of Prunella vulgaris extract and zinc sulfate is equivalent after 48 hours, and the mortality rate is above 95.44%; based on the growth condition of rainbow trout feeding on Prunella vulgaris extract, the specific growth rate of fish is significantly improved, the growth performance is improved, and the growth of fish is promoted; the plasma biochemical indexes after parasitic infection show that the addition of 0.25% to 1% Prunella vulgaris extract in feed can significantly reduce stress caused by pathogenic stress and protect the health of fish liver.

The invention discloses a feed formula suitable for aquaculture, and relates to the technical field of aquaculture. The feed comprises the following components in percentage by weight: 15-30% of soybean meal, 18% of rapeseed meal, 7% of cottonseed protein concentrate, 0-17% of sunflower seed meal, 4% of rice bran, 23% of flour, 4% of corn dry vinasse, 0.15-7.32% of microcrystalline cellulose, 0.2% of choline chloride, 3.8% of soybean oil, 1.5% of monocalcium phosphate, 1% of premix, 0-0.21% of lysine hydrochloride, 0-0.04% of methionine, 0.03% of a mildew preventive, 0.01% of an antioxidant and 2-5% of enteromorpha powder. The formula provided by the invention can promote ingestion of the grass carps under the condition of low soybean meal, improve the average weight, weight gain rate and specific growth rate of the grass carps, and promote absorption and utilization of phosphorus in the feed by the grass carps.

The present invention relates to a composition for improving skin health containing an active ingredient-enhanced green tea flower enzyme reactant, the enzyme reactant according to the present invention being generated by inducing an enzyme reaction by using a green tea flower extract as a substrate and using a green tea flower-derived crude enzyme solution in a specific growth period, thereby improving the content of an active ingredient and the enzyme activity. The composition has the advantage of being capable of exhibiting excellent skin health improving ability.

The invention relates to the technical field of microorganisms, in particular to lactobacillus reuteri and application thereof. The Lactobacillus reuteri is Lactobacillus reuteri ZAAS002, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on October 24, 2025, and the registration number of the preservation center is CGMCC (China General Microbiological Culture Collection Center) No.36328. The Lactobacillus reuteri is Lactobacillus reuteri ZAAS002. The lactobacillus reuteri produced by the lactobacillus reuteri ZAAS002 disclosed by the invention has an excellent antibacterial effect on staphylococcus aureus and escherichia coli; the bullfrog feed additive can significantly improve growth, increase bullfrog weight gain rate and specific growth rate, and reduce death rate, and is a good strain as a growth-promoting probiotic in bullfrog culture.

The invention belongs to the technical field of aquaculture, and provides application of dried porcine soluble as a feed protein source capable of replacing fish meal. According to the invention, dried porcine soluble is used as a feed protein source capable of replacing fish meal to be added into daily feeding feed for fishes, and under the condition that the replacement level is not more than 20%, growth indexes such as final weight, specific growth rate, protein efficiency and the like of the fishes have no significant difference from those of a whole fish meal group, and even are better than those of the whole fish meal group. Compared with fish meal, the dried porcine soluble is wide in source and low in cost, high-value utilization of heparin by-products can be achieved, dependence on fish meal resources can be relieved, and sustainable development of aquaculture is promoted.

This invention belongs to the field of seahorse farming technology and discloses a feed additive that promotes the growth of striped seahorses and its application. Specifically, it discloses a feed additive comprising carotenoids, sea squirt powder, oyster powder, mannan oligosaccharides, probiotics, and vitamins. This invention provides a feed additive containing carotenoids, sea squirt powder, oyster powder, mannan oligosaccharides, probiotics, and vitamins. Adding this feed additive to seahorse feed can significantly promote seahorse growth (significantly increasing weight gain rate, specific growth rate, and body length growth rate) and improve seahorse survival rate. This feed additive not only helps improve the economic benefits of seahorse farming but also provides strong technical support for the sustainable development of the seahorse farming industry.

The invention discloses a diamond crystal as well as a growth deposition device and a growth method thereof, and belongs to the technical field of vapor deposition. The diamond crystal meets the following conditions that a, the thickness difference between the edge area and the center area of the crystal is smaller than or equal to 10 micrometers; b, total dislocation defect density lt; 105 / cm < 2 >; c, the thickness of the crystal is 0.3 to 10 mm; and d, the absolute value of the stress is lower than 20 MPa. By using a structure for actively adjusting the height of the surface and the edge of the molybdenum support and combining a specific growth process, the growth method for realizing the constant height of the crystal surface and the edge of the molybdenum support and inhibiting the edge effect is realized, and the high-quality and few-crack diamond crystal is obtained.