85 results about "Specific growth" patented technology

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to promote the growth of soybean plants under flood stress. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then apply to the soil containing the soybean at a specific growth stage after germination. Optionally, the KHP solution can be diluted by water as disclosed in the application.

The embodiments disclose a method for testing organic plant materials, including detecting and associating machine-readable QR code identifiers at multiple testing locations within a facility, preparing samples at the multiple testing locations with microorganism-specific growth stimulator solutions to facilitate detection of contaminants, automatically detecting and analyzing environmental conditions at the multiple testing locations with a plurality of sensors, automatically correlating the captured images and the environmental conditions to the associated machine-readable QR code identifiers, analyzing and comparing the captured images and the environmental data against known harmful microorganisms, automatically generating contamination reports including detected infestations and remediation recommendations with mitigation actions and displaying the contamination reports and the recommendations and automatically generating and displaying a facility map on the graphical user interface with hotspot locations of the detected infestations of the contamination reports associated with the machine-readable QR code identifiers.

The utility model relates to the technical field of seed cultivation devices, and discloses a seed cultivation device for biological breeding, which comprises a constant-temperature incubator body, a first fixing shaft is rotatably connected to the inner side of the constant-temperature incubator body, a support frame is fixedly connected to one end of the first fixing shaft, a connecting frame is fixedly connected to the top of the support frame, and the connecting frame is fixedly connected to the bottom of the constant-temperature incubator body. A second fixing shaft is rotationally connected to the side wall of the connecting frame, a placement tray is fixedly connected to the outer side of the second fixing shaft, and a groove is formed in one side of the constant-temperature incubator body. The seed cultivation device for biological breeding solves the problems that when an existing seed cultivation device is used, seed cultivation pots are generally placed on a horizontal supporting plate, and the specific growth condition of each seed cultivation pot is difficult to see clearly through the horizontally-placed supporting plate when the number of the seed cultivation pots is large; and a user is difficult to control the breeding condition of the seeds in the culture pot.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

The present invention discloses a relay aquaculture method for Penaeus japonicus, and relates to the field of biological aquaculture. The method employs a mixture of a protein source, a fat source, a complex vitamin, a complex inorganic salt, an attractant, and (-)-caryophyllene oxide-modified xanthan gum to prepare a bait for the relay aquaculture of Penaeus japonicus, resulting in a good survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus. The method also employs glucosamine-modified humic acid, which is used in the preparation of an amino acid fertilizer paste, which is then used in the relay aquaculture of Penaeus japonicus, resulting in an even better survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The present invention discloses a method and apparatus for screening strains with high substrate utilization efficiency using CO2 release rate, belonging to the field of microbial screening. The method comprises: standardized strain culture: inoculating the strain to be screened onto a solid culture medium, controlling environmental parameters and culturing in the dark; dynamic CO2 monitoring: collecting CO2 concentration using a CO2 sensor with integrated temperature, humidity, and pressure compensation; metabolic signature extraction: calculating the CO2 release rate per unit time, the release rate slope, and the normalized cumulative release of hyphae; hyphae growth monitoring and data synchronization: calculating the colony specific growth rate and generating a synchronized data set with CER data; hyphae growth metabolic correlation analysis: establishing a coupled kinetic model of hyphae growth and CO2 release; efficiency prediction and strain screening: using a multivariate regression model to predict substrate utilization efficiency and screening efficient strains according to grading criteria. The present invention has the advantages of being non-destructive and highly real-time, significantly shortening the screening cycle for strains with high substrate utilization efficiency.

The invention relates to the technical field of aquaculture, in particular to an accurate regulation and control method for micropterus salmoides culture water quality. According to the micropterus salmoides culture water quality regulation and control method, a culture water body meets one or more of the following conditions: (1) adding photosynthetic bacteria into the culture water body every day, and controlling the final concentration of the photosynthetic bacteria in the water body to be 10 < 6 >-10 < 7 > CFU / mL; (2) adjusting the content of dissolved oxygen in the culture water body to 5.5-7.5 mg / L; (3) adjusting the temperature of the culture water to 26 + / -1 DEG C in the daytime and 23 + / -1 DEG C at night. The total nitrogen, total organic carbon and chemical oxygen demand can be obviously reduced through day and night temperature regulation and addition of photosynthetic bacteria, the water change demand is reduced, and the eutrophication risk is reduced; meanwhile, the specific growth rate (SGR) and the weight growth rate (WGH) are improved, the immune and antioxidant indexes are optimized, and the aeration energy consumption is reduced by controlling dissolved oxygen to be 7.0 mg / L.

To provide a paddy field water management device capable of appropriately managing the growth of rice plants in response to environmental changes such as weather conditions, without increasing the labor burden on a paddy field manager.SOLUTION: The device comprises: an irrigation schedule management unit configured to divide the rice growth period from transplanting to harvest into a plurality of growth stages, generate an irrigation schedule that defines irrigation modes for each growth stage, and update the irrigation schedule based on the accumulated temperature after the transplanting; and a paddy field water level control unit configured to remotely control water level control devices installed in respective paddy fields based on the irrigation schedule, so as to adjust water levels of the respective paddy fields according to the irrigation mode for each growth stage. The paddy field water level control unit is configured to, when rice reaches a specific growth stage according to the irrigation schedule, select whether to adjust the water levels of the respective paddy fields according to the irrigation mode corresponding to the specific growth stage, or to adjust the water levels of the respective paddy fields according to a different specific irrigation mode than the irrigation mode, based on temperature indices of the respective paddy fields.SELECTED DRAWING: Figure 2

This invention discloses an irrigation resource allocation optimization system based on environmental perception, relating to the field of agricultural irrigation technology. It includes: an environmental data acquisition module for real-time acquisition of soil moisture, air humidity, precipitation, light intensity, ambient temperature, and wind speed within the irrigation area, using the acquired data as a real-time environmental parameter set; and a crop growth stage determination module for determining the specific growth stage of the crop based on a preset crop growth cycle model and the real-time environmental parameter set. This invention uses the environmental data acquisition module to obtain multi-dimensional environmental parameters such as soil moisture and air humidity in the irrigation area in real time. The crop growth stage determination module determines the specific growth stage of the crop based on the preset crop growth cycle model and the real-time parameters. The baseline water requirement acquisition module then matches the baseline water requirement for the corresponding stage from a database, solving the problem in existing technologies that fail to connect the dynamic water requirements of the crop growth period with real-time environmental parameter fluctuations.

The invention belongs to the technical field of composite strains, particularly relates to a high-lignin straw fermentation composite microbial agent, and a preparation method and a fermentation process thereof, and aims to solve the problems of low lignin degradation efficiency, long period and high cost of a single microorganism in the prior art. According to the invention, a composite synergistic system of coriolus versicolor, bacillus subtilis and pichia pastoris is innovatively adopted, and efficient degradation is realized through a mechanism that the coriolus versicolor secretes a lignin degrading enzyme system, the bacillus subtilis assists cellulose decomposition and the pichia pastoris optimizes a microenvironment; the preparation method of the complex microbial inoculant comprises the following steps: culturing various strains in a proper culture medium to a specific growth state, and mixing according to an optimized volume ratio; the fermentation process relates to high-lignin straw pretreatment, inoculation, sealed fermentation under control conditions and regular ventilation, the lignin degradation rate can be increased, the feed nutritional value can be improved, the relative feeding value and quality can be improved, and economic and environment-friendly technical support is provided for resource utilization of agricultural by-products.

The invention relates to a stem cell technology, in particular to a pet stem cell induction culture medium, a preparation method and a culture method, and the culture medium significantly improves the proliferation efficiency and multidirectional differentiation potential of adipose-derived stem cells by optimizing the proportion of specific growth factors. The preparation method comprises the steps of basic culture medium preparation, growth factor addition and sterile filtration. Experimental verification shows that compared with a control group, the adipogenic differentiation efficiency of ADSCs cultured by the culture medium is improved by 40%, the osteogenic differentiation ALP activity is improved by 65%, and the positive rates of surface markers CD29, CD44 and CD90 are all greater than or equal to 95%. The problems of animal-derived pathogen pollution risk and low induction efficiency of a traditional culture medium are solved, and the culture medium is suitable for pet medical treatment.

The invention provides a prediction method of a cell culture cooling point and application thereof, and relates to the technical field of fermentation culture.The method comprises the steps that cell density data are obtained, the current specific growth rate mu current of cells is calculated after smoothing algorithm processing, and when mu current is smaller than or equal to a mu threshold value, it is judged that the cooling point is reached; the mu threshold value is equal to k * mu max; wherein the mu max is the maximum specific growth rate of the cells, the k is 0.4-0.6, and the mu max and the mu current are from the same batch of cells. According to the method, the specific growth rate mu of the cells is used as a credible process control decision variable for the first time, the hysteresis of a traditional living cell density method is overcome through robust data processing, prospective capture of the physiological state of the cells is achieved, and the product yield and the process robustness are remarkably improved.

This invention discloses a method for designing optimal strains for product synthesis using an enzyme-constrained model combined with an evolutionary algorithm, comprising the following steps: Step S1, solving the enzyme-constrained model with the objective function of maximizing the specific growth rate of the strain to obtain the simulated metabolic flux of the wild-type strain; Step S2, performing dimensionality reduction analysis and labeling on genes directly related to enzyme synthesis within the model by solving a series of flux balance analysis problems with fixed biomass synthesis rates; Step S3, predicting the yield of single-target editing; Step S4, using a genetic algorithm to search for combined targets after dimensionality reduction, adjusting relevant parameters to obtain the optimal gene editing combination strategy; Step S5, repeating the experiment to verify the stability of the algorithm. This invention utilizes genetic algorithms and enzyme-constrained models to discover non-intuitive gene editing combination strategies, providing a new method for constructing efficient microbial cell factories for synthetic chemicals, and demonstrating the potential of applying heuristic algorithms to strain optimization design.

The invention provides a method, a system, equipment and a medium for constructing a yeast high-temperature tolerance prediction model, and belongs to the field of microbial breeding, and the method comprises the following steps: determining a tolerance label based on a maximum specific growth rate, an ethanol yield and a final ethanol concentration, and obtaining transcriptome data and metabolome data of a saccharomyces cerevisiae strain, the metabolome data comprises the synthesis rate of the trehalose, and the synthesis rate of the trehalose is determined according to the net synthesis amount of the trehalose in the heat shock time of the saccharomyces cerevisiae strain; constructing feature vectors according to the transcriptome data and the metabolome data; and inputting the feature vectors into the model to determine a predicted tolerance score, and performing hyper-parameter optimization on the model according to the tolerance tag and the tolerance score of the saccharomyces cerevisiae strain in the training set to obtain a yeast high-temperature tolerance prediction model. According to the method, the built-in powerful nonlinear learning ability of the model is utilized, the complex mapping relation between the multi-omics characteristics and the high-temperature tolerance is mined, and the accuracy and reliability during model prediction are guaranteed.

The invention discloses an application of acetyl ursolic acid in promoting the growth of burkholderia. Eight Burkholderia strains are separated from soil, the separated eight Burkholderia strains are cultured in a nitrogen-free culture medium added with 50 mg / L of acetyl ursolic acid, and the result shows that the biomass of Burkholderia can be increased by 40%-95% compared with that of a control group without acetyl ursolic acid within 24 h through acetyl ursolic acid, the Burkholderia strain has a remarkable growth promoting effect on Burkholderia, and the Burkholderia strain has a good application prospect. The core functions such as nitrogen fixation are not influenced. The concentration gradient experiment further determines that the effective concentration range of the acetyl ursolic acid on the growth promotion of the burkholderia is 5-100 mg / L, and the optimal concentration of the acetyl ursolic acid is 25-75 mg / L. Acetyl ursolic acid is used as a specific growth promoting agent of burkholderia, not only are the problems of specificity, safety and stability in the prior art solved, but also the synergistic effect of promoting growth and improving crop quality is realized.

The present invention relates to a method for culturing lactic acid bacteria that adapt to the vaginal environment and strongly adhere to vaginal epithelial cells. The method comprises inoculating the cultured lactic acid bacteria seed solution into a liquid culture medium, and culturing the culture medium statically until the OD value of the culture medium reaches 0. 600 When the value is 0.3-0.6, the preparation is added and culture is continued, and after completion, the lactic acid bacteria that strongly adhere to vaginal epithelial cells are obtained; the preparation includes L-arabinose. The present invention also relates to the use of L-arabinose in preparing a preparation for improving the adhesion of lactic acid bacteria to vaginal epithelial cells. By adding the relevant preparation during a specific growth stage of lactic acid bacteria, it is beneficial to ensure rapid bacterial proliferation and stable formation of surface hydrophobic proteins, thereby further improving the adhesion and colonization ability of the cultured lactic acid bacteria.

This invention relates to the field of microbial technology, and particularly to a peanut-specific growth regulator and its application in sugarcane-peanut intercropping. The peanut-specific growth regulator of this invention consists of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-6 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L-0.4 g / L of brassinolide, and 0.2 g / L-0.4 g / L of jasmonic acid. At this concentration, the peanut-specific growth regulator can significantly increase peanut yield. Under sugarcane-peanut intercropping conditions, the yield increase is even more significant. This peanut-specific growth regulator can provide a new approach for the development of specialized agricultural biological agents.

The utility model discloses a vertical greening device for landscaping, which relates to the technical field of landscaping and comprises a mounting plate, a plurality of planting boxes are arranged on one side of the outer wall of the mounting plate, an adjusting mechanism is arranged on one side of the outer wall of the mounting plate, and the adjusting mechanism is used for adjusting the height of the planting boxes. Meanwhile, the distance between the upper and lower planting boxes can be adjusted. Through the arrangement of the adjusting mechanism, the distance between the upper planting box and the lower planting box can be adjusted according to the specific growth requirements of crops, so that the growth environment is optimized, the growth efficiency and the yield of the crops are improved, the adjacent mounting plates can be connected together through the arrangement of the sliding strips and the sliding grooves, and the planting box is convenient to use. The whole wind resistance of the device is improved, meanwhile, maintenance and replacement of a single mounting plate are facilitated, through arrangement of an irrigation mechanism, a mounting sleeve and a mounting pipe, plants in each planting box can be irrigated, and the labor amount of workers is reduced.

The present invention relates to a method for predicting the reaction rates of substances during anaerobic ethanol fermentation. This method uses changes in the specific growth rate μ to predict changes in the specific consumption or specific production rate of each substance under different substrate and product concentration conditions. Compared with the existing technology, the present invention, based on a process model constructed based on thermodynamic principles, can predict the stoichiometric relationship between the rates of raw materials, products, heat production, and other factors during the ethanol production process of brewer's yeast under anaerobic culture at different specific growth rates, based on minimal understanding of process metabolic regulation mechanisms and experimental data requirements. Based on this, the thermodynamically based black box model constructed in this solution can provide excellent predictions of changes in the anaerobic ethanol fermentation process under steady-state and dynamic conditions.

A growth prediction device includes a stage predictor configured or programmed to predict a growth stage for a crop, and a definer configured or programmed to define, when a period from a reference date to an accomplishment date of the growth stage includes a specific growth stage, a threshold value that indicates a prediction date for the specific growth stage with respect to transition data for the growth stage predicted by the stage predictor. When predicting a next growth stage, the stage predictor is configured or programmed to predict a date on which transition data for the next growth stage reaches the threshold value to be the specific growth stage.

The invention relates to the technical field of plant germplasm resource evaluation, and discloses a bamboo reed germplasm resource environmental adaptability evaluation method. The method comprises the following steps: acquiring environment data of a plurality of ecological test points and bamboo reed phenotype data, and forming a germplasm environment response record after matching; according to the bamboo reed key phenological period division points, the records are intercepted and recombined into a plurality of phenological response sub-records; for each sub-record, extracting an environmental factor extreme value exceeding a preset tolerance range and a duration, and constructing an environmental pressure portrait; connecting all portraits of the same bamboo reed germplasm in series at all test points and arranged according to a phenological sequence to form a cross-regional whole-growth-period environmental adaptability path diagram; and inputting the path diagram into a pre-trained multi-dimensional adaptability discrimination network for analysis, and outputting an adaptability grade classification label to finish evaluation. According to the method, environmental stress in a specific growth stage can be quantified more finely, the overall adaptability of germplasm to a complex stress sequence is systematically judged, and the accuracy and practicability of evaluation are improved.

The invention discloses a directional domestication method of anti-pollution algae species of haematococcus, and relates to the field of microbial breeding, key domestication indexes comprise specific growth rate, photochemical quantum yield, astaxanthin content and intracellular pollutant concentration; inputting the domestication key indexes into a resistance comprehensive scoring model, and outputting a resistance comprehensive score; and the pollutant concentration adjustment algorithm comprehensively scores according to the resistance of the current domestication period. According to the method, the resistance comprehensive scoring model is constructed, so that traditional qualitative resistance evaluation is upgraded to quantitative scoring. Scoring can objectively and comprehensively reflect the comprehensive resistance of the algal population, the problem of bifamily algal species caused by single index screening is avoided, and it is ensured that the finally obtained algal species have the anti-pollution capacity and the economic value at the same time. Whether the resistance comprehensive score reaches a target value or not is taken as a feedback signal, the pollutant concentration of the next period is automatically adjusted, the pressure is properly increased when the algal population is excellent in performance, and the pressure is timely reduced when the algal population is poor in performance. Compared with a traditional fixed gradient mode, the dynamic regulation and control mode can shorten the domestication period.

The present application relates to the technical field of plant germplasm resource evaluation, and discloses a reed germplasm resource environmental adaptability evaluation method. The method comprises the following steps: obtaining environmental data and reed phenotype data of multiple ecological test points, and matching to form a germplasm environmental response record; dividing the record according to a key reed phenophase, and recombining the record into multiple phenophase response sub-records; for each sub-record, extracting extreme values and duration of environmental factors that exceed a preset tolerance range, and constructing an environmental stress image; connecting all the images of the same reed germplasm at all test points in the order of phenophases to form a cross-regional whole growth period environmental adaptability path diagram; inputting the path diagram into a pre-trained multi-dimensional adaptability discrimination network for analysis, and outputting an adaptability grade classification label to complete the evaluation. The present application can more finely quantify environmental stress at a specific growth stage, and systematically discriminate the overall adaptability of germplasm to a complex adversity sequence, thereby improving the accuracy and practicality of the evaluation.

The invention discloses a culture solution for inducing proliferation and differentiation of mesenchymal stem cells and a preparation method of the culture solution, the culture solution comprises the following raw materials in parts by weight: 40-60 parts of a basal culture medium, 5-10 parts of a serum substitute, 3-5 parts of growth factors, 1-3 parts of hormones, 1-3 parts of vitamins, 0.5-1 part of a small molecule compound and 1-3 parts of other additives, and the invention relates to the technical field of stem cell culture. According to the culture solution for inducing proliferation and differentiation of the mesenchymal stem cells and the preparation method of the culture solution, by optimizing basic components and adding specific growth factors and small molecule compounds, the proliferation capacity and directional differentiation efficiency of the MSCs are remarkably improved, and the culture solution is suitable for in-vitro induction of various tissues such as bones, cartilages and nerves. The culture solution adopts an animal-origin-free formula, has the advantages of low cost, high safety, strong applicability and the like, and provides an important technical tool for the field of regenerative medicine.

This invention discloses a silty substrate vegetation concrete for tung tree seed propagation and its application method. The silty substrate vegetation concrete is made by mixing silty soil, cement, seawater, and fertilizer. By pouring this mixture into seedling tubes and planting tung tree seeds, an integrated concrete seedling tube structure is formed. This tube can be directly planted in outdoor wetlands for tung tree seed propagation, thus achieving seed germination, seedling propagation, and planting. This invention utilizes silty substrate vegetation concrete to construct an integrated concrete seedling tube structure, which has suitable strength, porosity, and density, improving the specific growth rate and survival rate of tung tree seedlings and greatly simplifying the tung tree seed cultivation process.

The utility model discloses a fish ecological breeding device which comprises a breeding pond, supporting columns are fixedly connected to the inner surface of the breeding pond, rotating motors are fixedly connected to the interiors of the supporting columns, rotating columns are fixedly connected to the output ends of the rotating motors, rotating rods are fixedly connected to the upper surfaces of the rotating columns, and the rotating rods are fixedly connected to the lower surfaces of the rotating columns. A mounting groove is formed in the lower surface of the rotating rod, positioning columns are fixedly connected to the left side and the right side of the inner surface of the mounting groove, rolling wheels are rotatably connected to the outer surfaces of the positioning columns, baffles are fixedly connected to the lower surface of the rotating rod, the number of the baffles is five, and screening holes are formed in the four baffles. By screening the fishes with different body types, the fishes with the same body type can be intensively bred, so that a more accurate breeding strategy can be formulated according to the specific growth stages and body type characteristics of the fishes, such as reasonably adjusting the feeding amount, optimizing the breeding density and the like, and differentiated management on the fishes at different growth stages is facilitated.

This invention provides a culture medium for closed-system culture of mesenchymal stem cells, comprising the following components: 1% (v / v) human platelet lysate, 10-15 g / L basal culture medium powder, 15-20 mM HEPES, 1.0-2.0 g / L sodium bicarbonate, and 0.1-1.0 g / L sodium chloride; wherein the basal culture medium powder includes non-essential amino acids, D-glucose, L-glutamine, specific growth factors, inorganic salts, and vitamins. Using the culture medium provided by this invention for mesenchymal stem cell culture eliminates the need for exogenous CO2, reducing equipment costs and process complexity; furthermore, it results in cell viability ≥97%, more concentrated particle size distribution, and more stable expression of surface markers, which is beneficial for large-scale production.

This invention discloses a recombinant protein pPIC9K-17, its preparation method, and its applications, relating to the field of gene recombination technology. The recombinant protein pPIC9K-17 provided by this invention does not strictly follow the (Gly-X-Y)n triple helix repeat pattern of traditional collagen in its core structural unit. The repeating unit sequence includes a "GAIQ" sequence and a sequence containing collagen characteristics, possessing a certain rigid structural basis and containing receptor-binding or signal activation domains for specific growth factors. The direct tandem connection of multiple such units theoretically enables multivalent binding, enhancing affinity and signal activation efficiency with cell surface receptors, thus surpassing the biological functional limitations of single components (pure collagen or pure growth factors) or simple repeating structures. The recombinant protein of this invention not only effectively promotes the proliferation and migration of skin fibroblasts and accelerates collagen synthesis, but also shows clear efficacy in promoting hair regeneration (hair growth), and can be used to prepare products for skin repair and / or promoting hair regeneration.