205 results about "Aquaculture industry" patented technology

The invention relates to the technical field of intelligent breeding monitoring, in particular to a breeding early warning method and system based on artificial intelligence image recognition. The method comprises the following steps of: 1, dynamically acquiring and adaptively preprocessing a multi-modal image; step 2, constructing a multi-branch convolutional neural network based on the preprocessed image data, respectively extracting space apparent characteristics and time behavior characteristics of a target object, and dynamically fusing characteristic weights by combining real-time environment parameters to generate an abnormal probability value; and step 3, performing multi-level dynamic early warning decision and feedback optimization, dynamically generating a multi-level early warning threshold according to environmental parameters and historical abnormal probability value distribution, triggering differential response operation, and updating parameters of the multi-branch convolutional neural network and the multi-level early warning threshold based on an artificial recheck result and equipment feedback data. The method can automatically adjust the early warning mechanism according to the change of the real-time environment, thereby effectively preventing the potential risk in the breeding process, improving the breeding management efficiency, and guaranteeing the sustainable development of the breeding industry.

The invention relates to the technical field of the Internet of Things, and more specifically discloses an intelligent aquaculture management system and method based on the Internet of Things, which can provide a fine management scheme, optimize aquaculture conditions and improve yield and efficiency for farmers by monitoring aquaculture environment parameters in real time through a sensor and combining data analysis and artificial intelligence. The risk is reduced, and sustainable development of the aquaculture industry is promoted.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention discloses an animal quarantine method, and aims to solve the problems of high omission ratio, early warning lagging and lack of environment optimization of the traditional quarantine means. According to the method, multi-dimensional biological signals such as the heart rate, the respiratory rate, the body temperature and the acceleration of an animal are collected in real time through an ear tag type wearable device, and the anomaly detection precision is improved through a self-adaptive sampling mechanism; meanwhile, the environment monitoring module collects the temperature, humidity and ammonia gas concentration of the farm, and dynamically regulates and controls the temperature, humidity and ammonia gas concentration in case of abnormality. And the edge computing node adopts a long-short-term memory network to fuse and analyze data, outputs an epidemic disease risk probability, and optimizes the model through self-learning. The embodiment shows that the method can perform early warning and environment optimization in the incubation period, the quarantine time is shortened to 5-10 minutes per head, the omission ratio is lower than 5%, and an efficient and accurate epidemic disease prevention and control solution is provided for the breeding industry.

The invention relates to the technical field of aquaculture, and discloses an intelligent aquaculture environment regulation and control method and system, and the method comprises the steps: collecting the original data of an aquaculture environment in real time through a plurality of sensors; historical breeding data are collected, and the historical breeding data are adopted for training to construct an aquaculture environment evaluation model; performing correction processing, missing value filling feature selection and dimension reduction processing on the original data to obtain preprocessed data, inputting the preprocessed data into an aquaculture environment evaluation model, and outputting an evaluation result of a current aquaculture environment through the aquaculture environment evaluation model; based on the evaluation result of the aquaculture environment, when it is judged that the current environment is not suitable for aquaculture, equipment connected with the pond is intelligently regulated and controlled; according to the system, blindness and low efficiency of traditional cultivation are eliminated, fine management of the cultivation environment is achieved, excellent growth conditions are provided for aquaculture organisms to the maximum extent, and the aquaculture industry is powerfully pushed to a new modernization and intelligent development stage.

The invention belongs to the field of biotechnology and aquaculture, and particularly relates to a method for improving hypoxia resistance of pelteobagrus vachelli based on hypoxia induction of liver extracellular vesicles of the pelteobagrus vachelli. The method comprises the following steps: extracting high-purity extracellular vesicles EVs in liver tissues of pelteobagrus vachelli subjected to low-oxygen treatment by adopting a method of combining differential centrifugation and high-precision iodixanol density gradient centrifugation; diluting the extracted EVs to 2mu g / mu L by using PBS (Phosphate Buffer Solution) to prepare an EVs suspension; an intraperitoneal injection mode is adopted, the EVs suspension is injected into the body of the pelteobagrus vachelli, and after injection is conducted for 24 hours, the hypoxia resistance of the pelteobagrus vachelli is evaluated through a suffocation point detection test. The invention opens up a brand new path for improving the hypoxia resistance of the pelteobagrus vachelli, provides a new idea and a new method for efficiently enhancing the hypoxia resistance of economic fishes in the field of aquaculture, is expected to play an important role in actual aquaculture production, and assists the sustainable development of the aquaculture industry.

The invention belongs to the technical field of microorganisms, and particularly relates to transgenic chlamydomonas capable of secreting antibacterial peptide outside cells as well as a construction method and application of the transgenic chlamydomonas. Specifically, the invention provides transgenic chlamydomonas capable of secreting antibacterial peptide outside cells. Tests prove that not only can the water quality be improved and the immunity of fishes be improved, but also the step of purifying the antibacterial peptide protein can be effectively omitted. The zebra fish experiment is carried out, and it is proved that the survival rate of the zebra fish can be increased. Meanwhile, compared with an escherichia coli and yeast expression system, the chlamydomonas reinhardtii can also be used as bait of aquatic organisms such as fishes and shrimps, can also be cultured in a closed culture pond, and has important application potential in the aquaculture industry.

The invention belongs to the technical field of biology, and discloses a megalobrama amblycephala hypoxia-resistant snp molecular marker and detection and application thereof, the number of SNP sites of the SNP molecular marker related to megalobrama amblycephala hypoxia-resistant characters is three, the SNP sites are located at the 14321966 nucleotide site, the 14323457 nucleotide site and the 14323789 nucleotide site of a megalobrama amblycephala reference genome CM032046.1 respectively, the polymorphisms are G / A, T / C and C / A respectively, and the SNP sites are named as SNP1, SNP2 and SNP3 respectively. Based on the SNP molecular marker, the hypoxia-resistant character of megalobrama amblycephala can be identified by detecting the genotype of the SNP site, and the hypoxia-resistant megalobrama amblycephala can be screened, so that the breeding of the hypoxia-resistant variety of megalobrama amblycephala is accelerated, and the stable development of the megalobrama amblycephala culture industry is promoted.

The invention relates to the technical field of biology, in particular to a method for relieving high-starch-induced liver injury and glucose metabolism disorder of perches. The feed formula provided by the invention comprises the following components in percentage by weight: 20-22% of cassava starch, 2-6% of mulberry leaf polysaccharide and the like. The mulberry leaf polysaccharide and high-content starch in the feed form efficient synergy, so that the growth performance of the weever is remarkably improved, accumulation of liver glycogen and fat is effectively reduced, and the health condition of the liver is remarkably improved; meanwhile, the blood sugar regulation capability of the weever is also optimized, the insulin resistance caused by high starch diet is reduced, and the insulin sensitivity is enhanced; and by activating a PI3K / Akt signal path and other mechanisms, a glucose metabolism path is optimized, and efficient utilization of energy is promoted. The feed disclosed by the invention is economical and efficient in formula, provides solid scientific support for healthy culture of perches, and powerfully promotes sustainable development of aquaculture industry.

The invention relates to a specific primer pair for sex identification of Chinese softshell turtles and an identification method thereof.The primer pair comprises two pairs of primers which are used for amplifying specific sequences in genome DNA of the Chinese softshell turtles respectively so as to achieve accurate sex identification, and according to the method, the genome DNA of a to-be-detected sample can be amplified through a PCR amplification reaction; when the amplification product is 1000 bp, the sex is judged, when the amplification product is 1000 bp and 2000 bp, the sex is judged, according to the identification method provided by the invention, standard PCR amplification conditions and a reaction system are adopted, high efficiency and accuracy of sex identification are ensured, the invention further provides a kit, and the kit can be used for detecting the sex. The kit comprises a reagent for extracting DNA (deoxyribonucleic acid), a primer combination and a reaction system required by PCR (polymerase chain reaction) amplification as well as an electrophoretic buffer solution and a labeling reagent required by agarose gel electrophoresis, is beneficial to improving the sex identification efficiency and accuracy of the Chinese softshell turtles, and is widely applicable to sex screening and other related researches in the aquaculture industry.

The invention relates to the technical field of data management systems and methods of classification numbers G06Q, and provides a traceable management system and method based on an aquaculture industry service platform. Comprising a server, a mobile data anchoring device, a detachable traceability label module, a life cycle state management module, an event hash and anchor verification module and a block chain intelligent verification module. The detachable traceability label module is used for identifying the identity and life cycle state of a breeding individual and triggering a state change event in a disassembly behavior; the mobile data anchoring device collects data of the key node and forms an anchor point record; the life cycle state management module maintains a life cycle logic state diagram of each individual and verifies whether uploading of all events is compliant or not; the event hash and anchor verification module encapsulates all behavior nodes into a structured hash event, and compares and verifies the structured hash event with a current state diagram to form a credible anchor; and the block chain intelligent verification module performs contract-level verification on the event hash and the state path.

The invention discloses an application of bortezomib in preparation of a medicine for resisting grouper iridovirus. Researches show that bortezomib has a remarkable inhibiting effect on grouper iridovirus, and is low in cytotoxicity and good in safety. The bortezomib can obviously reduce the fluorescence signal intensity of virus protein, inhibit the transcriptional level of main capsid protein MCP and envelope protein VP19 of the virus and reduce the copy number of DNA and mRNA of the virus; the bortezomib can effectively inhibit grouper iridovirus infection and effectively block synthesis of virus protein, so that efficient inhibition of grouper iridovirus infection is achieved, and along with prolonging of virus infection time, bortezomib can also remarkably inhibit virus gene transcription, protein synthesis and genome replication. Therefore, bortezomib not only can effectively prevent and treat grouper iridovirus infection, but also has the characteristics of high specificity, low toxicity, remarkable antiviral effect and the like, and also has important application value in prevention and control of iridovirus-related diseases in aquaculture industry.

The invention belongs to the field of tilapia mossambica breeding, and discloses a rapid and efficient tilapia mossambica breeding method and system.The method comprises the steps that tilapia mossambica populations with excellent character potential are collected and screened, and a basic breeding population is established; molecular markers related to target characters are developed, and individuals carrying excellent genes are rapidly screened; a target gene is precisely regulated and controlled through a CRISPR / Cas9 technology, so that stable inheritance of excellent characters is accelerated; phenotypes such as growth performance, disease resistance and environment resistance are efficiently evaluated and screened; the excellent characters are fixed; and the performance of the new strain under the actual breeding condition is evaluated. The efficient and low-cost tilapia mossambica breeding method is constructed by integrating molecular marker, gene editing and phenotype screening technologies, the breeding efficiency and the commercialization value of a new strain are remarkably improved, and powerful technical support is provided for the aquaculture industry.

The application discloses a Vibrio harveyi oral vaccine and a preparation method and application thereof. The application uses a lactococcus lactis NZ9000 expression system, modifies transformation parameters, effectively expresses Vibrio harveyi OmpU protein, constructs a lactococcus lactis (NZ9000) engineering bacterium expressing Vibrio harveyi outer membrane protein (OmpU), develops an oral vaccine against Vibrio harveyi infection, aims to reduce the infection of economic animals in seawater aquaculture by oral administration, provides a new scheme for dealing with the harm of Vibrio harveyi infection and antibiotic resistance and the like, promotes the breeding of fry and fingerlings, and promotes the development of biological agriculture and related industries. The strategy is expected to overcome the problems of traditional injection vaccines, such as inconvenient operation and great stress, and can provide a new technical scheme for reducing the dependence of the aquaculture industry on antibiotics and promoting the establishment of a green and healthy breeding mode.

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

The invention discloses a micropterus salmoides intestinal function feed additive based on composite synergy and application thereof, and belongs to the technical field of feed additives. The feed additive for improving the intestinal function of the largemouth bass comprises the following raw materials: clostridium butyricum, tributyrin, aspergillus niger and a plant source carrier. The invention provides a compound feed additive capable of remarkably improving the growth performance of the largemouth bass, improving the digestive enzyme activity of the intestinal tract and optimizing the intestinal barrier, and the compound feed additive can remarkably improve the weight gain rate and the specific growth rate of the largemouth bass, remarkably improve the digestive enzyme activity of the intestinal tract of the largemouth bass and maintain the integrity of the tissue structure of the intestinal tract. Compared with a single additive, the compound additive shows a better synergistic gain effect in the aspect of comprehensive effects, has important value for promoting research, development and practical application of novel feed additives of micropterus salmoides, has good popularization and application prospects, and is expected to bring remarkable economic benefits to the breeding industry.

The invention relates to an application of Edwardsiella fish-sourced protein EseO or a coding gene thereof in preparation of medicines for treating and / or preventing obesity. The edwardsiella fish source protein EseO screened by the invention can inhibit lipid metabolism of cells HeLa and mice C57 / BL6J, which proves that the edwardsiella fish source protein EseO has an obvious fat reducing effect and can be applied to feed additives, medical products and the like in the aquaculture industry related to weight loss.

The present invention provides a method for dynamic hierarchical prediction of water temperature based on high-level pond aquaculture, wherein high-level pond water parameters including at least water temperature data and air temperature data and external meteorological data are input into a multimodal fusion adaptive collaborative network, and a dynamic merging method is used to segment the time signal set into time segments; and a prediction model TimeFusionNet fused with a progressive attention mechanism is used to perform time series prediction to obtain prediction results. The present invention provides a set of targeted water temperature prediction schemes for high-level pond aquaculture environments, which can be widely used in high-level pond aquaculture environments, provide a refined water temperature control scheme for the aquaculture industry, and improve aquaculture efficiency. At the same time, the scheme can also provide important data support for environmental monitoring and ecological research, and has high application value and social benefits.

In recent years, the aquaculture industry and the ocean ship industry develop rapidly, the requirements for materials such as ships, underwater equipment, fishponds and fishing gears are higher and higher, and the requirements for the performance of the materials are higher and higher. The aquatic product equipment needs to be soaked in water throughout the year, and the requirements for corrosion resistance and adhesion of materials of the aquatic product equipment are high; and meanwhile, higher requirements on the antifouling and self-cleaning capabilities of the material are also put forward. A mainstream super-amphiphobic material taking organosilane as a coupling agent is strict in construction condition, needs to be carried out at specific temperature and humidity, is relatively high in cost, and is not beneficial to sustainable recovery development due to lack of reprocessing or recovery capability. According to the super-amphiphobic composite material for preparing the primer by using the zirconate as the coupling agent, provided by the invention, the adhesion of a coating, especially metal, can be effectively enhanced, the corrosion resistance is remarkably improved, meanwhile, the cost is reduced, and the economy of fishermen engaged in marine aquatic products and even the aquaculture industry is improved.

The invention discloses application of ocean-derived enterococcus durans YQ-6 in improving the growth performance, slaughter performance and muscle quality of Leizhou black ducks, and belongs to the technical field of poultry breeding. Experiments prove that the marine-derived enterococcus durans YQ-6 can improve the growth performance and muscle quality of the Leizhou black ducks, enhance the immune function and oxidation resistance of organisms, improve the intestinal flora structure of the Leizhou black ducks and improve the intestinal health, and the effect is optimal when 1 * 10 < 9 > CFU / kg of the marine-derived enterococcus durans YQ-6 is added into feed. The feed disclosed by the invention complies with the development trend of'feed resistance-free ', and has important significance on green, safe and sustainable development of the breeding industry.

The invention discloses a sea cucumber accurate fishing robot suitable for a shallow sea culture area, and relates to the technical field of aquaculture equipment.The sea cucumber accurate fishing robot comprises a rack, an equipment cabin, a collecting cabin, a power supply cabin and a plurality of propellers are installed on the rack, and a fishing mechanical arm matched with the collecting cabin is installed on the front side of the rack; a precise fishing framework is arranged in the equipment cabin, the precise fishing framework identifies the positions of sea cucumbers in the shallow sea culture area and controls the fishing mechanical arm to fish the sea cucumbers and then put the sea cucumbers into the collecting cabin, and a power supply unit is arranged in the power supply cabin to supply power to the precise fishing framework and the fishing mechanical arm. Through fusion perception, turbid water body image enhancement and accurate positioning, full-process autonomous accurate fishing of the sea cucumbers in the shallow sea high-turbidity environment is achieved, manual diving operation is replaced, the fishing efficiency and the product quality are greatly improved, and intelligent upgrading of the sea cucumber culture industry is promoted.

The invention discloses an ADO (adenosine dioxygenase) gene deleted zebrafish model as well as a construction method and application thereof. According to the invention, ADOa gene knockout is carried out on a model organism (zebrafish) to successfully construct an ADO gene deleted zebrafish model. Based on the model, the function of the ADO gene in fish taurine metabolism can be researched more deeply, so that a new scientific basis is provided for understanding the ADO function of zebra fish and even other fishes, and meanwhile, the model can be used for drug screening and provides a theoretical basis and technical support for breeding and genetic improvement of aquaculture industry.

The invention discloses a real-time estimation method for tail water flow of aquaculture. The method comprises the following steps: firstly, comprehensively collecting basic environment data of geographic position, landform, water quality and geometric dimension of an aquaculture pond, and arranging and storing the data; secondly, monitoring the initial flow velocity, flow direction and cross sectional area change of the water flow at the tail water discharge port in real time by using professional equipment, and transmitting data according to preset intervals; then analyzing interference factors such as wind power, tide and cultivated organism activity, constructing a correlation model to correct water flow state parameters, and preliminarily estimating instantaneous flow based on a water flow dynamics algorithm; and predicting a flow change trend through time sequence analysis, and constructing an incidence matrix optimization estimation value in combination with production activity data. And finally, the accurate estimated value is transmitted to a culture management system database in a JSON format through a Modbus protocol, and key data support is provided for scientific management. According to the method, a more scientific, efficient and accurate solution is provided for estimating the tail water flow in the aquaculture industry.

The present invention discloses novel antimicrobial peptides based on large pre-trained models and their applications, belonging to the field of bioengineering technology. The antimicrobial peptides AMP3, AMP6, AMP13, AMP19 and AMP23 provided by the present invention have broad-spectrum antibacterial properties and have great application value in the development and preparation of antibacterial agents. They can be applied in the aquaculture industry as feed additives, and can also be developed into antibacterial agents and antibacterial drugs, etc., with broad application prospects.

The invention discloses a molecular marker primer for identifying genetic sex of pike fish and application thereof, and relates to the technical field of aquatic animal breeding. The invention provides a molecular marker primer for identifying genetic sex of pike fish and application of the molecular marker primer. The molecular marker primer for identifying the genetic sex of the pike comprises a molecular marker specific primer and an auxiliary molecular marker primer, and the molecular marker primer for identifying the genetic sex of the pike is applied to all-female breeding of the pike. Compared with the prior art, the molecular marker with extremely high correlation with the genetic sex of the pike vibrio molitor and the genetic sex of the pike vibrio molitor is adopted, the difference between the molecular marker and the prior art is that the molecular marker is universal in genetic sex identification of the pike vibrio molitor and the genetic sex of the pike vibrio molitor, and in addition, the identification accuracy of the molecular marker reaches up to 100% and is obviously superior to that of the prior art. Aiming at the problems of high all-female breeding cost and low efficiency caused by shortage of a dog fish pseudo-male fish screening technology, the invention provides a dog fish genetic sex identification marker and a set of efficient and convenient dog fish all-female breeding method which is formed by taking the dog fish genetic sex identification marker as a core, so that the development of the dog fish breeding industry is effectively promoted.

This invention belongs to the field of agricultural and aquatic organism detection technology, specifically relating to a fluorescent RPA detection kit and its application method for detecting Ichthyophthirius multifiliis. Compared to traditional PCR technology, the detection kit and method of this invention significantly simplify the operation steps. Furthermore, after RPA amplification, the results can be directly interpreted through real-time fluorescence signals, eliminating the need for subsequent analysis operations such as opening the lid or electrophoresis. This avoids cross-contamination between different amplification products and reduces false positive results, greatly improving detection efficiency and accuracy. In aquaculture, this innovative RPA kit will significantly enhance the rapid on-site detection capability of Ichthyophthirius multifiliis, providing an efficient, simple, low-cost solution that does not require cold chain transportation, meeting the urgent need of modern aquaculture for rapid and accurate pathogen detection.

The invention discloses a culture method based on posthypoxia gastric intima separation treatment of freshwater white pomfret, and relates to the technical field of aquaculture and disease prevention and control. The method comprises the following steps: step 1, identifying hypoxia symptoms; 2, medicine compatibility feeding is carried out; 3, regulating and controlling the water body environment; 4, breeding environment management; and step 5, continuous monitoring. According to the invention, the food maternal, the endothelium corneum gigeriae galli and the pipemidic acid are combined for the separation treatment of the endogastric membranes of the aquatic animals for the first time, the thinking limitation of traditional disease prevention and treatment is broken through, the traditional Chinese and western medicine theories are organically combined, and a new disease prevention and treatment thought is provided for the aquaculture industry; compared with a traditional pure antibiotic treatment or water quality improvement method, the scheme is high in pertinence and directly aims at a core pathological link of gastric intima separation after hypoxia; the comprehensive treatment effect is good, and meanwhile, anti-infection, mucous membrane repair and function regulation are considered; and the used medicines are all common medicines, have small side effects and low residues, and meet the safety requirements of aquatic products.

The present application relates to a kind of circadian rhythm-based circulating water aquaculture fish pond dissolved oxygen mechanism modeling method, including the influence of fish respiration consumption, water surface atmospheric reoxygenation, circulating water flow, mechanical aeration factor on dissolved oxygen dynamic change;According to oxygen transfer equation, considering the influence of the above four factors on fish pond dissolved oxygen, the dissolved oxygen mechanism model of circulating water aquaculture fish pond is established;Combined with oxygen transfer equation and the characteristics of fish respiratory activity changes between day and night, the influence of fish respiratory circadian rhythm on the model is analyzed, and the model is verified.The present application can more accurately reflect the high and low peak value in different time periods and also capture the dynamic changes presented in the whole cycle, and effectively avoid the loss and risk caused by the inaccurate model description to the aquaculture industry in extreme breeding environment or abnormal environment.

The invention discloses an application of alpha-lipoic acid in preparation of an anti-iridovirus drug. Related experiments show that alpha-lipoic acid can safely and effectively treat iridovirus such as frog virus type 3, not only can effectively reduce harm of iridovirus to frog and fish culture, but also can promote ecological high-quality development of aquaculture industry, and has obvious economic and social benefits.

This invention discloses a specific DNA fragment, primers, and applications for sex identification of the longfin sclerotium. PCR amplification and agarose gel electrophoresis were performed using PCR primers. The results showed that individuals with a specific 271bp band were male, while those without a band were female. This invention discloses a molecular marker, primers, and identification method for sexing the longfin sclerotium. Sexing can be determined by taking fin rays without killing the fish; it can also identify the sex of juvenile fish whose sex cannot be determined by appearance. It has advantages such as accuracy, simplicity, speed, and minimal harm to the fish, providing important assistance for the conservation of wild longfin sclerotium resources and the development of the aquaculture industry.