282 results about "Siniperca chuatsi" patented technology

The invention provides a primer group, a kit and an identification method for rapidly identifying genetic sex of siniperca chuatsi and application, and relates to the technical field of molecular biology. The primer group comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID NO. 1, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 2. The primer group can accurately distinguish the male and female sex of siniperca chuatsi at the genome level, so that the physiological genetic sex of sex reversal individuals is rapidly identified. The establishment of the method provides an important scientific basis for the development of a subsequent siniperca chuatsi unisexual breeding technology and the research of a sex determination mechanism.

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The utility model discloses a mandarin fish domestication feeding device which comprises a feeding pipeline and a bait temporary storage tank arranged at the upper end of the feeding pipeline, a feeding hopper is arranged at the upper end of the bait temporary storage tank, and the feeding pipeline comprises a vertical pipe with the upper end in butt joint with the bait temporary storage tank and a horizontal discharging pipe in butt joint with the bottom of the vertical pipe. One end of the horizontal discharge pipe is connected with a high-pressure water source or air source; the vertical pipe is of a multi-section telescopic rod structure. A cathode ring piece and an anode ring piece are arranged at the two ends of the vertical pipe, and the cathode ring piece and the anode ring piece are connected with a pulse generator used for outputting pulse signals and a pulse power amplifier used for controlling output voltage through wires. The fish feeding device can achieve feeding at different depths, can feed live fish, dead fish and feed according to different proportions, is simple and convenient to operate, greatly reduces the workload of manual feeding, and can be widely applied to the field of bait feeding.

The invention relates to the technical field of aquaculture, in particular to an on-demand intelligent feeding management system and method based on aquaculture, and the system obtains the ingestion activeness of siniperca chuatsi in each time period in each density of culture environment through analysis according to ingestion state parameters of the siniperca chuatsi in each time period in each density of culture environment. And constructing a time period-ingestion activity degree curve under each density, so as to obtain the optimal feeding time period of the siniperca chuatsi under the breeding environment of each density, and analyzing to obtain the positive influence degree of the breeding environment of each density on the ingestion activity degree of the siniperca chuatsi, the ingestion behavior growth correlation value of the siniperca chuatsi and the ingestion activity degree of the siniperca chuatsi. The optimal feeding amount of siniperca chuatsi in the breeding environment of each density is determined, the improvement effect evaluation index of aquaculture is obtained according to the water quality evaluation coefficients and the breeding cost of the experimental breeding ponds of each density and the control breeding ponds of each density, and the improvement effect evaluation index is fed back, so that blind feeding is avoided, the feeding accuracy is improved, and the breeding environment is optimized.

The invention discloses a processing method capable of shortening the fermentation period and reducing the biogenic amine content for inoculated fermentation of smelly mandarin fish, and belongs to the field of food processing. The method comprises the following steps: (1) pretreating mandarin fish; (2) preparing fermentation liquor, wherein the fermentation liquor is prepared from NaCl, Chinese prickly ash, a leavening agent and water; the mass percentage concentration of each component is as follows: 0.1% of pepper, 4-8% of NaCl, 0.5-1.5% of a leavening agent and the balance of water; (3) fermentation process of smelly mandarin fish: completely immersing the fish body in the fermentation liquid, respectively fermenting for 6-12 days at the temperature of 5-20 DEG C, and collecting a smelly mandarin fish sample; and (4) performing vacuum packaging: performing vacuum packaging on the collected smelly mandarin fish samples, and performing cryopreservation at-18 DEG C. The leavening agents are lactobacillus sake, pediococcus pentosaceus, lactobacillus plantarum and mixed strains (lactobacillus sake, pediococcus pentosaceus and lactobacillus plantarum in a ratio of 1: 1: 1) respectively. The fermentation period of the siniperca chuatsi is shortened through inoculated fermentation, the content of biogenic amine is reduced, and the method has very high application and popularization value.

The invention relates to the technical field of freshwater fish culture, and discloses a culture box for freshwater fish culture, which comprises a culture box and a feeding tank, the top of the feeding tank is fixedly connected with a feeding hopper, and a feeding mechanism is arranged in the feeding tank; the feeding mechanism comprises a motor, a vertical shaft, a disc, movable teeth and fixed teeth; the motor is fixedly connected to the outer wall of the top of the feeding tank, the output end of the bottom of the motor penetrates into the feeding tank, animal livers in the smashing chamber are smashed into small blocks through friction and collision of movable teeth and fixed teeth, swallowing of the mandarin fish fries is facilitated, growth and development of the mandarin fish fries are facilitated, a bent pipe is driven by a disc to rotate, and the feeding efficiency is improved. The feeding range is expanded through the rotating bent pipe, and the gear and the rack are arranged to move back and forth in the rotating process of the bent pipe, so that the feeding range of the bent pipe is further expanded, mandarin fish fries are prevented from being grabbed by adult fishes during concentrated feeding, mandarin fish ingestion is more uniform, and the overall mandarin fish growth efficiency is improved.

The invention relates to the technical field of fishery breeding, in particular to a step depth adjustable mandarin fish domestication breeding water temperature detection device and a domestication method.The step depth adjustable mandarin fish domestication breeding water temperature detection device comprises a mounting plate; a lifting assembly capable of driving a lifting piece arranged on the mounting plate to intermittently descend in the longitudinal direction is arranged on the mounting plate; triggering pieces are arranged on the mounting plate at equal intervals. A temperature measuring cylinder is arranged on the lifting part, the temperature measuring cylinder is communicated with a water sample extracting assembly on the lifting part, the water sample extracting assembly comprises a suction cylinder which is arranged on the lifting part and is communicated with the temperature measuring cylinder, a suction part is arranged in the suction cylinder, and the suction part is matched with a driving assembly on the lifting part and can suck a water sample into the temperature measuring cylinder; when the driving assembly is separated from the triggering piece and is quickly reset, the suction piece acts reversely, and the sample pumped into the temperature measuring cylinder is discharged; through cooperation of all the assemblies, culture personnel can conveniently and accurately obtain the temperatures of water bodies at different depths of a culture pond.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The invention belongs to the technical field of mandarin fish culture, and discloses a mandarin fish recirculating aquaculture method and system based on sensing data analys.The mandarin fish recirculating aquaculture method comprises the steps that a culture pond is divided into n areas, and environment parameters of aquaculture water of all the areas are sequentially collected based on sensors; the environmental parameter consistency coefficient of each region is calculated in sequence according to the collected environmental parameters, whether the environmental parameters of the aquaculture water body of each region are abnormal or not is judged according to the environmental parameter consistency coefficient, if yes, the adjusting quantity of the abnormal environmental parameters is calculated, and the corresponding environmental parameters are adjusted according to the adjusting quantity. If the environmental parameters are not abnormal, determining the weight data of the mandarin fish, analyzing the growth state of the mandarin fish according to the weight data of the mandarin fish, then adjusting the feeding amount of the mandarin fish based on the growth state, and monitoring and analyzing the environmental parameters of the culture water body and the growth state of the mandarin fish in real time to realize precise culture and improve the culture efficiency.

The invention belongs to the technical field of immunology, and particularly relates to an anti-mandarin fish immune globulin D (IgD) monoclonal antibody as well as a preparation method and application thereof. The invention provides a hybridoma cell strain 5C7 capable of secreting an anti-fish IgD monoclonal antibody or an antigen binding fragment thereof for the first time, the hybridoma cell strain 5C7 is preserved in China Center for Type Culture Collection on January 16, 2025, and the preservation number is CCTCC NO: C202550. The anti-mandarin fish IgD monoclonal antibody or the antigen binding fragment thereof can be subjected to specific reaction with mandarin fish secreting type IgD protein and B cell surface film type IgD, can be applied to detection of IgD in a mandarin fish serum sample, and can also be applied to detection of IgD + B cell surface markers.

The invention relates to the technical field of industrial aquaculture, and discloses a mandarin fish living body automatic grading method suitable for industrial aquaculture, which comprises the following steps: constructing a grading channel with gradually increased cross section area, and injecting constant water flow to form a flow field with gradually decreased flow velocity gradient; according to the method, mandarin fish individuals with different swimming capacities autonomously select adaptive flow velocity sections to reside, spatial layering is achieved, and then zoned collection is achieved, and through the synergistic effect of the flow field gradient and fish behavior instincts, traditional mechanical classification is converted into a stress-free autonomous standing process; meanwhile, the grading precision under the high-density working condition is ensured by means of an eddy current correction mechanism induced by a wedge-shaped groove of a flow guide grid, lossless evaluation of the physiological activity is achieved by multiplexing a flow field pulsation signal, and a multi-dimensional grading decision basis is provided for industrial aquaculture.

The invention relates to the technical field of fry domestication, in particular to domestication bait and domestication method for mandarin fish fries. According to the specific technical scheme, the domestication bait for the mandarin fish fries comprises live crucian carp fries and silver carp fries, half-dead crucian carp fries and half-dead silver carp fries, mandarin fish powder, a mixture of the dead crucian carp fries and the dead silver carp fries and mandarin fish feed 0 #; a phagostimulant is added into the powder, and the phagostimulant is one of squid paste, betaine and hermetia illucens pulp. According to the three phagostimulants disclosed by the invention, the feeding domestication efficiency can be improved, the squid paste has the most obvious effect in the aspect of promoting the early growth performance and is excellent in related physiological metabolism indexes, and the betaine relieves the liver metabolism burden in the mandarin fish domestication process.

The invention relates to the technical field of mandarin fish classification, and discloses a weighing platform for mandarin fish classification and distribution, which comprises a conveyor belt rotatably connected with a roller, the roller is rotatably connected with the inner side of a frame, the inner side of the frame is provided with a plurality of sliding bearing plates, the bearing plates tightly press the top of an electronic scale, the electronic scale is fixedly connected with the inner side of the frame, and a visual sensor is arranged above the electronic scale. A rotating shaft of the stepping motor is fixedly connected with a guide plate; one side of the guide plate is provided with a guide slideway; the inner side of the guide slideway is provided with a protection plate; the bottom of the guide slideway is provided with a buffer plate; the bottom plate is fixedly connected with the inner side of the guide slide way, so that the buffer plate can provide an effective buffer effect when mandarin fish slides down, the damage to the fish body caused by the fact that the fish body collides with the inner wall of the storage basket at a high speed is reduced, the risk of fish body injury is reduced, and economic losses are reduced for suppliers.

The invention discloses an intensive ecological mandarin fish breeding system in a photovoltaic composite environment, which comprises a photovoltaic breeding area, an intelligent management and control center and an energy collaboration module, and is characterized in that a photovoltaic power station array is arranged in a space above the photovoltaic breeding area; the photovoltaic culture area is provided with at least one industrial culture group, each industrial culture group comprises a plurality of metal culture ponds, and each culture pond is of a round pond structure with an inverted conical bottom and a central sewage collection opening; the plurality of metal culture ponds are communicated through pipelines or diversion structures, and the intelligent management and control center comprises a data acquisition layer, a platform decision-making layer and an application control layer; the data acquisition layer is used for acquiring culture environment data in real time and comprises a water quality monitoring sub-module, a meteorological monitoring sub-module and a video monitoring sub-module; a mandarin fish growth regulation and control model is arranged in the platform decision-making layer, and the model is configured to be capable of processing data uploaded by the data acquisition layer and at least executing one of the following decisions.

The device comprises a heat preservation outer shell, an installation inner shell is installed on the inner side of the heat preservation outer shell, a liquid drainage frame is installed on the inner side of the installation inner shell, and a placement mesh plate is installed on the upper surface of the liquid drainage frame; the liquid drainage frame is arranged in a spiral shape, the longitudinal section of the liquid drainage frame is in a V shape, and a plurality of hole-shaped structures are formed in the bottom of the installation inner shell. A liquid guide pipe installed in the installation inner shell is arranged on the upper side of the liquid drainage frame, the liquid guide pipe is arranged in a spiral shape, and a plurality of atomization nozzles are installed on the upper side of the liquid guide pipe. The smelly mandarin fish pickling device belongs to the technical field of smelly mandarin fish pickling, and achieves the technical effects that the spiral arrangement of the liquid drainage frame and the cavity formed between the liquid drainage frame and the placement mesh plate are used for draining water, so that effective water drainage and moisture preservation in the pickling process are realized, and impurity water is prevented from entering fish meat after being pickled again; therefore, the pickling efficiency and the pickling quality are improved.

The utility model discloses a mandarin fish domestication flushing wave making device which comprises a water inlet pipeline used for achieving water inlet and stretching into a culture pond, a wave making pipeline is arranged at the end, away from the water inlet end, of the water inlet pipeline, and a water inlet fixing assembly used for fixing the position of the water inlet pipeline is arranged on the water inlet pipeline. A wave making supporting assembly used for fixing the position of the wave making pipeline is arranged on the wave making pipeline; the wave making pipeline comprises a wave making pipe corresponding to the culture pond, a plurality of water spraying holes are formed in the wave making pipe side by side at intervals, connecting pieces used for connecting the wave making pipe with other pipelines are arranged at the two ends of the wave making pipe, and the wave making pipe can change the directions of the water spraying holes in the connecting pieces. The direction of the water spraying holes in the wave making pipe can be changed through the connecting piece, and the wave making size, flow speed, frequency and direction can be changed through the variable frequency pump and the flow adjusting valve together, so that the mandarin fish wave making device meets the feeding characteristic requirements of mandarin fish in different growth stages.

The invention belongs to the technical field of mandarin fish culture, and relates to a mandarin fish domestication survival rate prediction method and system based on ecological simulation. Establishing a quantitative relation between the ecological factors and the sample mandarin fish survival rate change slope based on the quantitative relation so as to form an evaluation model; a historical survival rate curve is generated, and the risk state is determined by comparing the current sample mandarin fish survival rate change slope with the slope of the historical survival rate curve in the corresponding time section; calculating a change path of the ecological factors in a future time period based on the evaluation model, generating a predicted survival rate curve, and outputting an analysis report containing a risk state judgment result; according to the method, a complex domestication process is converted into a quantifiable data model, and early-stage accurate early warning of the risk is realized through dynamic analysis of the change trend of the survival rate.

The invention discloses an intelligent screening device for industrial aquaculture of mandarin fish, and belongs to the field of aquaculture, the intelligent screening device for industrial aquaculture of mandarin fish comprises a screening structure, the screening structure comprises a support, the lower portion of the rear end of the support is fixedly connected with an isolation device, and the lower portion of the front end of the support is fixedly connected with a linkage device; the lower end of the middle of the support is fixedly connected with two guiding structures, each guiding structure comprises a plurality of driving devices which are sequentially and fixedly connected from top to bottom, the upper portion of the rear end of each guiding structure is fixedly connected with an air interchanger, and the front end of each guiding structure is fixedly connected with a push rod. Through the screening structure, the mandarin fish can be screened without leaving the water surface, and the driving device can drive the mandarin fish with the size smaller than that of the round hole to pass through the isolation device through the threatening of sound and bubbles, so that the mandarin fish is not easy to cause stress reaction due to separation from the water surface when the mandarin fish is screened.

The utility model relates to cleaning equipment for smelly mandarin fish processing, which belongs to the technical field of smelly mandarin fish processing and comprises a conveyor, two groups of conveying components are oppositely arranged above the conveyor, two connecting plates are oppositely arranged above the middle of the conveyor, the bottom ends of the connecting plates are fixedly connected with side plates of the conveyor, and a motor is fixedly mounted on one side of each connecting plate. A transmission assembly is arranged between the connecting plates, and a cleaning mechanism is arranged below the transmission assembly in a matched mode. By means of the conveyor and the conveying rollers in the conveying assembly, mandarin fish can be conveyed in the state that the belly of the mandarin fish faces upwards, and the effect of cleaning the belly of the mandarin fish in the later period is improved; the cleaning block capable of being opened and closed is arranged in the cleaning mechanism, and the brush is fixedly installed on the cleaning block, so that on one hand, intermittent water outlet of cleaning water can be achieved, water resource saving is effectively improved, on the other hand, black membranes in the belly of mandarin fish can be cleaned, and the cleaning efficiency is improved. The mandarin fish cleaning effect is effectively improved.

The invention belongs to the field of electronic information and aquaculture fry, and particularly relates to a method for early domestication of siniperca chuatsi juveniles based on an artificial intelligence technology and a matched special feed for domestication of the siniperca chuatsi juveniles. When the siniperca chuatsi juveniles grow to 1 cm, domesticating the siniperca chuatsi juveniles with special puffed pellet feed under the assistance of an artificial intelligence technology. The artificial intelligence technology strictly monitors the water quality condition, the feeding behavior and the fry health condition in the domestication stage, the domestication progress of mandarin fish is more accurately controlled, and the special feed for mandarin fish juvenile domestication is complete in formula, good in palatability and higher in nutritive value and metabolism efficiency. According to the method, artificial intelligence is applied to the mandarin fish domestication process for the first time, early domestication of the mandarin fish juveniles is achieved for the first time, the time for starting domestication of the mandarin fish is advanced from 5-7 cm to 1 cm, and the time for receiving artificial feed by the mandarin fish is advanced from 10 cm or above to 2-3 cm. The controllability of baits in the mandarin fish fry breeding period is improved, meanwhile, the bait cost is saved, and technical support is provided for mandarin fish feed controllable breeding.

The invention discloses seedling and breeding equipment for mandarin fish culture, and relates to the technical field of aquaculture equipment. The invention provides a siniperca chuatsi breeding device with high survival rate, which comprises a box body and the like, the box body is fixedly connected with a temperature control plate, the box body is fixedly connected with paired air pumps, the box body is fixedly connected with paired spray heads, the air pumps are fixedly connected and communicated with the spray heads, the box body is fixedly connected with paired fixing frames which are longitudinally distributed, the fixing frames are rotatably connected with connecting shafts, and the connecting shafts are fixedly connected with the box body. The connecting shaft is fixedly connected with pairwise colored ribbons, the box body is fixedly connected with a partition plate, the partition plate is rotatably connected with pairwise hinged plates which are longitudinally distributed, and pairwise torsional springs are fixedly connected between the hinged plates and the partition plate. By means of the colored ribbons, the hinged plates and other parts, the activity degree of the mandarin fish fries can be improved, the fries of different specifications can be distinguished and separated by means of habits of the mandarin fish fries, the situations that the fries grab food and are disable in food due to the difference of the specifications of the fries are avoided, and the breeding survival rate of the seedling breeding equipment for mandarin fish breeding is increased.

The invention discloses a mandarin fish prefabricated dish detection method and system and a computer readable storage medium. The mandarin fish prefabricated dish detection method comprises the following steps: acquiring a gel strength original value, an elasticity original value, a water activity original value, an RGB image, a temperature value and a bacteria amount original value of a sample; preprocessing the elasticity original value, the moisture activity original value, the RGB image and the bacteria amount original value to form a first feature vector and a second feature vector corresponding to the sample; calculating the first feature vector and the second feature vector by using a preset neural network model to obtain a first qualified weight corresponding to the sample; on the basis of the original gel strength value, the temperature value and a gel strength correction model, calculating a gel correction strength value corresponding to the sample, and on the basis of the gel correction strength value, obtaining a second qualified weight corresponding to the sample; and on the basis of the first qualified weight and the second qualified weight, calculating a qualified coefficient of the sample, and outputting a second signal value that the sample is unqualified.

The invention provides a culture method of Weissella fusiformis RM125 and application of the Weissella fusiformis RM125 in mandarin fish culture, a probiotic strain is named as Weissella fusiformis RM125, is identified as Weissella, is preserved in China General Microbiological Culture Collection Center on November 11, 2024, and has a preservation number of CGMCC NO.32556. The invention further provides a preparation method of the Weissella fusiformis RM125. The strain can be stably planted in the intestinal tract of the feed siniperca chuatsi, can significantly improve the intestinal tract health of the siniperca chuatsi, significantly promotes the growth of the siniperca chuatsi, and reduces

The invention relates to the field of fish culture, and discloses a mandarin fish culture domestication management system and method based on factory circulating water, and the system comprises a circulating water treatment module which is used for achieving the circulating flow and purification treatment of a culture water body; comprising at least one culture pond and one or more water quality purification devices. The environment control module comprises a temperature control device and an oxygenation device and is used for adjusting the water temperature in the culture pond and increasing the dissolved oxygen content in the water body so as to maintain the environment condition suitable for growth of the mandarin fish; the automatic feeding module comprises an automatic feeding machine, corresponding feed is fed according to the growth stage, the feeding habit and the health condition of the mandarin fish, and smooth transition of the mandarin fish from natural feeding habits to artificial feed is achieved by gradually adjusting the feeding mode and the feed type; and the intelligent monitoring and management module is used for monitoring the breeding environment parameters and the growth state parameters of the mandarin fish in real time, analyzing monitoring data and then providing a corresponding breeding adjustment strategy according to an analysis result.

The invention relates to the technical field of siniperca chuatsi breeding, and provides the following technical scheme that a siniperca chuatsi breeding parasite ultrasonic wave-medicament atomization disinfecting and killing device comprises a paddling boat and an ultrasonic generator; an ultrasonic generator is installed in the middle of the interior of the paddling boat, a balancing weight is installed on one side of the ultrasonic generator, and a storage battery is installed on the other side of the ultrasonic generator. By arranging an angle adjusting structure, when parasites in siniperca chuatsi cultivation are killed, a reciprocating screw rod and a screw sleeve are matched for use, a toothed plate can be driven to reciprocate, the toothed plate is meshed with a rotating gear, so that an atomizing spray head on one side of a mounting seat is driven to rotate towards one side, and the atomizing spray head is driven to rotate towards the other side through the reciprocating motion of the toothed plate; the angle of the atomizing nozzle can be adjusted by adjusting the angle of the atomizing nozzle, so that the atomizing nozzle sprays pesticide in a fan shape when spraying the pesticide, the spraying area is increased, the reciprocating frequency of a pesticide spraying ship is reduced, and the working efficiency is improved.

The invention discloses a preparation method of a fish belly prefabricated dish, which comprises the following steps: preparing circulating water culture facilities of mandarin fish, adjusting water before culture, selecting fish fries, disinfecting before placing the fish fries, preparing fish fry bait formula, determining feed feeding rule, preparing before processing the mandarin fish, slaughtering and processing the mandarin fish, pickling the fish belly, and making the fish belly prefabricated dish. According to the preparation method of the fish belly prefabricated dish, efficient connection of breeding and processing is achieved, freshness and quality of a fish source are ensured, a water transfer process and biological membrane culture are optimized, a good water quality environment is created for mandarin fish, disease occurrence is reduced, the survival rate and growth quality of the fish are improved, and a scientific bait formula and feeding rules are adopted; according to the method, the siniperca chuatsi can grow quickly, the siniperca chuatsi can reach ideal weight and meat quality, high-quality fish belly premade dishes can be stably produced through strict quality control and standardized processes, and the siniperca chuatsi premade dishes have high market competitiveness.

The utility model provides tumbling equipment for salting smelly mandarin fish, which comprises a bearing frame, a mounting frame is hinged to one end of the top of the bearing frame, electric cylinders hinged to the mounting frame are symmetrically hinged to the top of the bearing frame, a mounting hole is formed in the mounting frame, and fixing rings are symmetrically fixed on the mounting frame and located in the mounting hole. The same tumbling cylinder is rotationally mounted in the two fixing rings, a material opening communicated with the interior of the tumbling cylinder is formed in one side of the tumbling cylinder, and partition plates are fixedly mounted on the inner side wall of the tumbling cylinder in an annular array mode. After the smelly mandarin fish and the pickling material are rolled, kneaded and mixed, the mounting frame rotates on the bearing frame through the two electric cylinders, so that the rolling and kneading barrel inclines downwards along the direction of the material opening, and the material in the rolling and kneading barrel can be completely poured out through the material opening; the situation that part of materials are left in the tumbling cylinder and need to be manually discharged by workers is effectively avoided, use is convenient, the workload of the workers is reduced, and the working efficiency is improved.

The invention discloses a nursing method before and after artificial spawning induction of parent mandarin fish, which comprises the following steps: nursing after collection of wild mandarin fish: selecting healthy, disease-free and residue-free fish, carrying out aeration transfer with water in a sorting box, controlling the temperature difference between water temperatures before and after transfer not to exceed 5 DEG C, soaking in a bag for over-temperature, soaking in saline water, and putting the wild mandarin fish into a 1: 1 filtered circulating water system; the steps of disinfection, pest killing, sterilization and bacteriostasis are alternately carried out, the ingestion condition, the swimming capacity and the clustering condition of the mandarin fish are observed, sampling microscopic examination observation is carried out, and if all conditions are normal and do not have parasitic diseases or bacterial infection, nursing work after the wild mandarin fish is collected is completed. The method further comprises the steps of prenatal breeding of mandarin fish, spawning induction, egg squeezing, postnatal breeding of parent fish and disease control, the survival rate of the wild mandarin fish after collection is improved in multiple aspects, breed conservation and breeding work of the wild mandarin fish is facilitated, the survival rate of the parent fish of the mandarin fish reaches 80% or above, and the method plays an important role in breeding of excellent new varieties which grow fast and are high in stress resistance.

The present invention provides a production method of high-quality room-temperature cooked stinky mandarin fish, including thawing, washing and cutting, soaking and sterilizing, removing odor and locking freshness, low-temperature air drying, deep-frying and shaping, sterilization and other processing of fermented stinky mandarin fish to obtain a room-temperature cooked stinky mandarin fish product. Compared with the traditional method, the present invention adopts a method of soaking and sterilizing combined with mild heat treatment sterilization to kill microorganisms, prolong the shelf life of cooked stinky mandarin fish, and can be stored at room temperature for 9 months, and significantly improve its processing quality, such as the volume shrinkage ratio of fish meat is reduced by 14.73% and the elasticity is increased by 80.24%. The present invention also innovates the formula and process for removing odor and locking freshness during the processing process. The added deodorizing and locking freshness ingredients have multiple functions such as removing odor, locking freshness and anti-oxidation, such as the product moisture content and water holding capacity are increased by 9.32% and 17.84% respectively; and improves the safety quality of cooked stinky mandarin fish during storage, such as the thiobarbituric acid content in the fifth month under 30°C storage conditions is significantly reduced by 43.39%.

The application discloses a mandarin fish domestication pool, which comprises a pool body, wherein an oxygenation device, a water inlet and a water outlet are arranged in the pool body, a bait fish breeding frame is arranged below the water inlet, and a feeding device is arranged on the side of the bait fish breeding frame; the feeding device comprises a feeding pipe, a bait temporary storage tank arranged on the upper end of the feeding pipe, a feeding hopper arranged on the upper end of the bait temporary storage tank, cathode ring pieces and anode ring pieces arranged in the feeding pipe, and a pulse generator and a pulse power amplifier connected through wires. The domestication pool is improved, so that live fish feeding, live fish and dead fish or feed proportionate feeding are facilitated, and switching and adjustment are facilitated, thereby improving the convenience and success rate of domestication.