182 results about "Siniperca chuatsi" patented technology

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The utility model discloses a mandarin fish domestication feeding device which comprises a feeding pipeline and a bait temporary storage tank arranged at the upper end of the feeding pipeline, a feeding hopper is arranged at the upper end of the bait temporary storage tank, and the feeding pipeline comprises a vertical pipe with the upper end in butt joint with the bait temporary storage tank and a horizontal discharging pipe in butt joint with the bottom of the vertical pipe. One end of the horizontal discharge pipe is connected with a high-pressure water source or air source; the vertical pipe is of a multi-section telescopic rod structure. A cathode ring piece and an anode ring piece are arranged at the two ends of the vertical pipe, and the cathode ring piece and the anode ring piece are connected with a pulse generator used for outputting pulse signals and a pulse power amplifier used for controlling output voltage through wires. The fish feeding device can achieve feeding at different depths, can feed live fish, dead fish and feed according to different proportions, is simple and convenient to operate, greatly reduces the workload of manual feeding, and can be widely applied to the field of bait feeding.

The invention relates to the technical field of fishery breeding, in particular to a step depth adjustable mandarin fish domestication breeding water temperature detection device and a domestication method.The step depth adjustable mandarin fish domestication breeding water temperature detection device comprises a mounting plate; a lifting assembly capable of driving a lifting piece arranged on the mounting plate to intermittently descend in the longitudinal direction is arranged on the mounting plate; triggering pieces are arranged on the mounting plate at equal intervals. A temperature measuring cylinder is arranged on the lifting part, the temperature measuring cylinder is communicated with a water sample extracting assembly on the lifting part, the water sample extracting assembly comprises a suction cylinder which is arranged on the lifting part and is communicated with the temperature measuring cylinder, a suction part is arranged in the suction cylinder, and the suction part is matched with a driving assembly on the lifting part and can suck a water sample into the temperature measuring cylinder; when the driving assembly is separated from the triggering piece and is quickly reset, the suction piece acts reversely, and the sample pumped into the temperature measuring cylinder is discharged; through cooperation of all the assemblies, culture personnel can conveniently and accurately obtain the temperatures of water bodies at different depths of a culture pond.

The invention relates to the technical field of industrial aquaculture, and discloses a mandarin fish living body automatic grading method suitable for industrial aquaculture, which comprises the following steps: constructing a grading channel with gradually increased cross section area, and injecting constant water flow to form a flow field with gradually decreased flow velocity gradient; according to the method, mandarin fish individuals with different swimming capacities autonomously select adaptive flow velocity sections to reside, spatial layering is achieved, and then zoned collection is achieved, and through the synergistic effect of the flow field gradient and fish behavior instincts, traditional mechanical classification is converted into a stress-free autonomous standing process; meanwhile, the grading precision under the high-density working condition is ensured by means of an eddy current correction mechanism induced by a wedge-shaped groove of a flow guide grid, lossless evaluation of the physiological activity is achieved by multiplexing a flow field pulsation signal, and a multi-dimensional grading decision basis is provided for industrial aquaculture.

The invention discloses an intensive ecological mandarin fish breeding system in a photovoltaic composite environment, which comprises a photovoltaic breeding area, an intelligent management and control center and an energy collaboration module, and is characterized in that a photovoltaic power station array is arranged in a space above the photovoltaic breeding area; the photovoltaic culture area is provided with at least one industrial culture group, each industrial culture group comprises a plurality of metal culture ponds, and each culture pond is of a round pond structure with an inverted conical bottom and a central sewage collection opening; the plurality of metal culture ponds are communicated through pipelines or diversion structures, and the intelligent management and control center comprises a data acquisition layer, a platform decision-making layer and an application control layer; the data acquisition layer is used for acquiring culture environment data in real time and comprises a water quality monitoring sub-module, a meteorological monitoring sub-module and a video monitoring sub-module; a mandarin fish growth regulation and control model is arranged in the platform decision-making layer, and the model is configured to be capable of processing data uploaded by the data acquisition layer and at least executing one of the following decisions.

The utility model discloses a mandarin fish domestication flushing wave making device which comprises a water inlet pipeline used for achieving water inlet and stretching into a culture pond, a wave making pipeline is arranged at the end, away from the water inlet end, of the water inlet pipeline, and a water inlet fixing assembly used for fixing the position of the water inlet pipeline is arranged on the water inlet pipeline. A wave making supporting assembly used for fixing the position of the wave making pipeline is arranged on the wave making pipeline; the wave making pipeline comprises a wave making pipe corresponding to the culture pond, a plurality of water spraying holes are formed in the wave making pipe side by side at intervals, connecting pieces used for connecting the wave making pipe with other pipelines are arranged at the two ends of the wave making pipe, and the wave making pipe can change the directions of the water spraying holes in the connecting pieces. The direction of the water spraying holes in the wave making pipe can be changed through the connecting piece, and the wave making size, flow speed, frequency and direction can be changed through the variable frequency pump and the flow adjusting valve together, so that the mandarin fish wave making device meets the feeding characteristic requirements of mandarin fish in different growth stages.

The invention belongs to the technical field of mandarin fish culture, and relates to a mandarin fish domestication survival rate prediction method and system based on ecological simulation. Establishing a quantitative relation between the ecological factors and the sample mandarin fish survival rate change slope based on the quantitative relation so as to form an evaluation model; a historical survival rate curve is generated, and the risk state is determined by comparing the current sample mandarin fish survival rate change slope with the slope of the historical survival rate curve in the corresponding time section; calculating a change path of the ecological factors in a future time period based on the evaluation model, generating a predicted survival rate curve, and outputting an analysis report containing a risk state judgment result; according to the method, a complex domestication process is converted into a quantifiable data model, and early-stage accurate early warning of the risk is realized through dynamic analysis of the change trend of the survival rate.

The invention discloses a mandarin fish prefabricated dish detection method and system and a computer readable storage medium. The mandarin fish prefabricated dish detection method comprises the following steps: acquiring a gel strength original value, an elasticity original value, a water activity original value, an RGB image, a temperature value and a bacteria amount original value of a sample; preprocessing the elasticity original value, the moisture activity original value, the RGB image and the bacteria amount original value to form a first feature vector and a second feature vector corresponding to the sample; calculating the first feature vector and the second feature vector by using a preset neural network model to obtain a first qualified weight corresponding to the sample; on the basis of the original gel strength value, the temperature value and a gel strength correction model, calculating a gel correction strength value corresponding to the sample, and on the basis of the gel correction strength value, obtaining a second qualified weight corresponding to the sample; and on the basis of the first qualified weight and the second qualified weight, calculating a qualified coefficient of the sample, and outputting a second signal value that the sample is unqualified.

The invention relates to the technical field of siniperca chuatsi breeding, and provides the following technical scheme that a siniperca chuatsi breeding parasite ultrasonic wave-medicament atomization disinfecting and killing device comprises a paddling boat and an ultrasonic generator; an ultrasonic generator is installed in the middle of the interior of the paddling boat, a balancing weight is installed on one side of the ultrasonic generator, and a storage battery is installed on the other side of the ultrasonic generator. By arranging an angle adjusting structure, when parasites in siniperca chuatsi cultivation are killed, a reciprocating screw rod and a screw sleeve are matched for use, a toothed plate can be driven to reciprocate, the toothed plate is meshed with a rotating gear, so that an atomizing spray head on one side of a mounting seat is driven to rotate towards one side, and the atomizing spray head is driven to rotate towards the other side through the reciprocating motion of the toothed plate; the angle of the atomizing nozzle can be adjusted by adjusting the angle of the atomizing nozzle, so that the atomizing nozzle sprays pesticide in a fan shape when spraying the pesticide, the spraying area is increased, the reciprocating frequency of a pesticide spraying ship is reduced, and the working efficiency is improved.

The utility model provides tumbling equipment for salting smelly mandarin fish, which comprises a bearing frame, a mounting frame is hinged to one end of the top of the bearing frame, electric cylinders hinged to the mounting frame are symmetrically hinged to the top of the bearing frame, a mounting hole is formed in the mounting frame, and fixing rings are symmetrically fixed on the mounting frame and located in the mounting hole. The same tumbling cylinder is rotationally mounted in the two fixing rings, a material opening communicated with the interior of the tumbling cylinder is formed in one side of the tumbling cylinder, and partition plates are fixedly mounted on the inner side wall of the tumbling cylinder in an annular array mode. After the smelly mandarin fish and the pickling material are rolled, kneaded and mixed, the mounting frame rotates on the bearing frame through the two electric cylinders, so that the rolling and kneading barrel inclines downwards along the direction of the material opening, and the material in the rolling and kneading barrel can be completely poured out through the material opening; the situation that part of materials are left in the tumbling cylinder and need to be manually discharged by workers is effectively avoided, use is convenient, the workload of the workers is reduced, and the working efficiency is improved.

The application discloses a mandarin fish domestication pool, which comprises a pool body, wherein an oxygenation device, a water inlet and a water outlet are arranged in the pool body, a bait fish breeding frame is arranged below the water inlet, and a feeding device is arranged on the side of the bait fish breeding frame; the feeding device comprises a feeding pipe, a bait temporary storage tank arranged on the upper end of the feeding pipe, a feeding hopper arranged on the upper end of the bait temporary storage tank, cathode ring pieces and anode ring pieces arranged in the feeding pipe, and a pulse generator and a pulse power amplifier connected through wires. The domestication pool is improved, so that live fish feeding, live fish and dead fish or feed proportionate feeding are facilitated, and switching and adjustment are facilitated, thereby improving the convenience and success rate of domestication.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The utility model discloses an efficient scaling and cleaning device for mandarin fish processing, and relates to the technical field of fishery processing tools, the efficient scaling and cleaning device comprises a grip and a protective cover installed at the front end of the grip, a motor is installed in the grip, and an output shaft of the motor is connected with a driving rotating shaft through a coupler; the right end of the interior of the protective cover is rotationally connected with a rotating connecting shaft, the connecting shaft is connected with a tool bit clamp, the driving rotating shaft is connected with one end of a scaling assembly, and the other end of the scaling assembly is clamped by the tool bit clamp. The tool bit clamp convenient to disassemble is arranged, when the tool bit is disassembled, the positioning bolt is screwed out firstly, then the tool bit penetrates into the hole in one side of the protective cover, fingers pinch the lap lugs, then the connecting shaft sleeve can be pulled outwards, limiting to the tool bit is relieved, and the tool bit clamp is good in tool bit clamping stability, convenient to disassemble and more convenient to use.

1. Name of the product in this design: Packaging Box (Stinky Mandarin Fish). 2. Purpose of this design: For packaging products. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to a rapid mandarin fish sex monitoring system based on ultrasonic image feature recognition, which comprises a multi-modal data acquisition module, an integrated high-frequency ultrasonic probe (10-15MHz), a near infrared spectrum module (850nm) and a visible light camera, and is used for synchronously acquiring an ultrasonic image, blood oxygen distribution data and a mandibular contour image of a mandarin fish belly gonad area. The rapid mandarin fish sex monitoring system based on the multi-mode ultrasonic image and intelligent analysis has a high-precision identification effect, the ultrasonic texture features are combined with near-infrared blood oxygen distribution data, the sex judgment accuracy is improved to be larger than or equal to 95%, and the misjudgment rate is reduced to be smaller than 3%.

The invention belongs to the field of stinky mandarin fish production, and provides stinky mandarin fish artificial inoculation bacterial colony fermentation equipment and a fermentation method, and the stinky mandarin fish artificial inoculation bacterial colony fermentation equipment accelerates the fermentation process through artificial inoculation of specific bacterial colonies, accurate control of the fermentation environment by using the fermentation equipment and combination of staged accurate temperature control, effectively solves the problem of long traditional fermentation period, and improves the production efficiency. Synchronous fermentation and full contact of the bean products and the mandarin fish are realized by utilizing the equipment; fermentation conditions are controlled, so that nutrition of the bean product fully participates in fermentation and is fused with the mandarin fish leavening, and the taste richness and the flavor layering sense of the smelly mandarin fish are remarkably improved.

1. Name of this design product: Lure Bait (Siniperca chuatsi). 2. Purpose of this design product: Lure bait for fishing. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: main view.

The invention belongs to the technical field of immunology, and particularly relates to a mouse anti-siniperca chuatsi secretory immunoglobulin T monoclonal antibody as well as a preparation method and application thereof. The invention provides a hybridoma cell strain 6B7 capable of secreting an anti-siniperca chuatsi secretory immunoglobulin T (sIgT) monoclonal antibody or an antigen binding fragment thereof for the first time, the hybridoma cell strain 6B7 is preserved in China Center for Type Culture Collection on May 13, 2025, and the preservation number is CCTCC NO: C2025161. The anti-siniperca chuatsi IgT monoclonal antibody or the antigen binding fragment of the anti-siniperca chuatsi IgT monoclonal antibody can be subjected to specific reaction with siniperca chuatsi secreting type IgT protein, and can be applied to detection of IgT in a siniperca chuatsi serum sample.

The invention discloses a fishing and light integrated mandarin fish ecological breeding method of a large-span flexible heat preservation greenhouse. The method comprises the following steps: (a) building the large-span flexible heat preservation greenhouse; (b) offspring seed putting; (c) regulating and controlling the water temperature of the mandarin fish pond; (d) bait feeding: feeding mandarin fish pellet feed along the bank after the mandarin fish fries are fed in each season; and (e) catching the mandarin fish by adopting a pull net. According to the fishing and light integrated flexible support photovoltaic and large-span flexible heat preservation greenhouse culture system, mandarin fish fry stocking, precise feeding of matched feed and fine water temperature regulation are combined; the problems of low fry survival rate, low growth speed, low feed conversion rate, high disease occurrence rate, difficulty in fishing, unobvious synergy and the like in a traditional mandarin fish culture mode are solved, facility efficient culture of mandarin fish is realized, integrated design is realized, a large-span flexible heat preservation greenhouse is arranged by utilizing photovoltaic pile positions, the construction cost of a temperature control greenhouse can be remarkably reduced, and the economic benefit is increased. And a real fishing and light integrated effect is achieved.

The utility model relates to the technical field of smelly mandarin fish processing, in particular to a bagging device for smelly mandarin fish processing, which comprises a conveying belt arranged in an inner cavity of a rectangular fixing frame, a packaging bag anti-toppling mechanism is arranged in the inner cavity of the rectangular fixing frame, and the packaging bag anti-toppling mechanism comprises a plurality of conveying boxes fixed on a working face of the conveying belt at equal intervals. Two U-shaped stop levers are symmetrically and fixedly installed on the two inner side walls of the rectangular fixing frame and located above the conveying box. A vacuumizing mechanism is arranged at the top end of the rectangular fixing frame and comprises a vacuumizing pump fixed to the top end of the rectangular fixing frame, a vacuumizing pipe at one end of the vacuumizing pump extends to an inner cavity of the rectangular fixing frame, and clamping plates for supporting packaging bags are fixedly installed in the inner cavity of the rectangular fixing frame and located on the two sides of the vacuumizing pipe. According to the packaging bag conveying device, packaging bags are kept in a vertical state all the time in the conveying process through the limiting effect of the conveying box and the two U-shaped stop levers, and then the packaging bags are prevented from toppling over in the conveying process.

The utility model provides a smelly mandarin fish soaking pool with an automatic lifting function, which comprises a soaking tank body, two groups of spraying assemblies are fixedly arranged at the top of the soaking tank body, a pressing assembly is fixedly arranged at the top of the soaking tank body, the pressing assembly is positioned between the spraying assemblies, a water storage tank body is fixedly arranged on one side of the soaking tank body, and the water storage tank body is fixedly arranged on the other side of the soaking tank body. A water passing opening is formed between the water storage tank body and the soaking tank body, a water supply assembly is arranged in the water storage tank body, the output end of the water supply assembly is connected with the spraying assembly in an assembled mode, and a flow guide cavity is fixedly formed in one side of the interior of the soaking tank body through a baffle; and a material taking assembly extending into the flow guide cavity is fixedly mounted on one side of the soaking tank body. According to the utility model, the pond realizes the effect of automatically extracting aquatic products through the material taking assembly, the aquatic products are effectively extracted and guided out along with the trend by utilizing the circulation effect of the aquatic products in the pond, and compared with the traditional manual salvage, the automatic aquatic product salvage device is faster and more convenient.

The embodiment of the application discloses a kind of based on low salt domestication and partition feeding mandarin fish fry scale-up cultivation method.The method is by providing low salinity domestication pool, and mandarin fish fry is put into the low salinity domestication pool, simultaneously based on light-flow composite stress domestication food process, using partition feeding and intelligent bait conversion system to mandarin fish fry is converted food, after mandarin fish fry is completed conversion food, hierarchical water quality regulation is carried out and micro-ecological steady state is constructed, until mandarin fish fry is out of pool, and is transferred into fish culture, to solve mandarin fish fry conversion food difficult, survival rate is low, and disease frequently occurs etc.Industrialization pain point, for realizing mandarin fish large specification fry scale-up, standardization, green production provides complete technical scheme, with wide popularization prospect and significant economic, social, ecological benefits.

The application provides a seven-day period factory cyclic Megalobrama amblycephala fry behavior domestication method and system, relates to the Megalobrama amblycephala breeding technical field, and includes: domesticating the fry into a domestication pond on the first day; starting vertical water flow impact before feeding fresh bait fish according to a preset first feeding frequency on the second day; feeding electric shock disabled live bait according to a preset second feeding frequency on the third day; feeding mixed live bait according to a preset third feeding frequency on the fourth day; feeding mosaic bait according to a preset fourth feeding frequency on the fifth day; feeding pure artificial compound feed using a composite feeding mode and starting water flow impact on the sixth day; feeding pure artificial compound feed on the seventh day, and screening domestication successful individuals based on behavior indexes after the period ends, so that the Megalobrama amblycephala is rapidly domesticated from feeding live bait to feeding artificial compound feed.

The invention discloses a method for visually detecting mandarin fish iridovirus and infectious spleen and kidney necrosis virus based on RPA-CRISPR Cas12a / Cas13a, and belongs to the technical field of biological diagnosis. According to the method, a CRISPR / Cas system is combined with RPA amplification, a fluorescent reporter and a test strip are used for naked eye observation, and the method combines the advantages of different isothermal amplification technologies, has the advantages of short detection time, high specificity, no cross reaction, high sensitivity and the like, realizes rapid and accurate diagnosis of the two pathogens, and has a good application prospect. Important technical support is provided for prevention and control of mandarin fish diseases.

The invention discloses a mandarin fish fry whole-stage feed taming device and a taming method thereof, and relates to the field of mandarin fish taming, the mandarin fish fry whole-stage feed taming device comprises a feeding barrel and a water inlet pipe, a stirring cavity is formed in the feeding barrel, a plurality of rotating blades are rotatably connected in the stirring cavity, a first driving part is arranged on the feeding barrel, and a live bait bin and a feed bin are arranged on the feeding barrel; a first discharging opening is formed in one side of the live bait bin, a second discharging opening is formed in one side of the feed bin, and pushing devices are arranged on the live bait bin and the feed bin respectively and used for pushing live bait to be discharged and pushing feed to be discharged respectively; a water adding pipe is arranged on the feeding barrel, a water adding switch is arranged on the water adding pipe, a discharging pipe is arranged at the bottom of the feeding barrel, a switch piece is arranged on the discharging pipe, the side wall of the discharging pipe is communicated with a feeding pipe used for feeding the feeding pond, and a control assembly used for controlling connection and disconnection of the feeding pipe and the discharging pipe is arranged on the feeding pipe. According to the invention, the eating amount of live baits by mandarin fish is increased, the efficiency of domesticating mandarin fish is improved, and the success rate of domesticating mandarin fish is increased.

The application discloses application of a dinoflagellate toxin in preparation of a medicine for resisting SCRV virus. The dinoflagellate toxin is Patinopecten yessoensis toxin. The application proves through experiments that the Patinopecten yessoensis toxin can significantly inhibit replication and infection of the SCRV virus, reduce virus titer and copy number, and destroy virus particle structure in a safe concentration range. Therefore, the Patinopecten yessoensis toxin can be used as an SCRV inhibitor for preventing and treating diseases caused by the SCRV of mandarin fish and perciform fish.

The invention relates to an ecological mandarin fish breeding method based on natural breeding of bait fishes, and belongs to the technical field of aquaculture. According to the method, a crayfish culture pond or a river crab culture pond serves as an ecological mandarin fish culture pond, and aquatic plants with the coverage rate larger than 30% are planted. Propagation and growth characteristics of indigenous crucian carps are ingeniously utilized, the indigenous crucian carps are put from November of the previous year to January of the next year, and feed residues and excrement for breeding crayfishes or river crabs are used for providing food for the crucian carps; the aquatic plants provide inhabiting and molting places for the crayfishes or the river crabs, provide spawning places for the crucian carps, and purify water to form a stable ecological system. Large-size mandarin fish fries are put in the middle and last ten days of May in the next year, natural breeding of crucian carps provides continuous and palatable live bait fishes for mandarin fish, an efficient ecological circulation system is formed, and rapid growth of mandarin fish is promoted. The usage amount of the water quality improver and fishery medicine is zero, and mandarin fish, crayfish or river crab and crucian are harvested in autumn at the same time.