693 results about "Culture environment" patented technology

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of mouse embryonic fibroblast feeder cells to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by defined components added to the culture environment that support rapid proliferation without differentiation. The medium contains an isotonic buffer, a blend of essential nutrients such as protein and lipids, and an effective growth factor or combination of factors that promote proliferation while inhibiting differentiation. Culturing human embryonic stem cells in fresh medium on an extracellular matrix according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

The invention provides a shrimp-fish-shellfish-alga multi-unit culture and aquatic organism regulation system thereof. A shrimp culture zone, a fish culture zone, a shellfish culture zone, a large scale alga cultivation zone, a probiotics and microalga culture zone, a water processing zone and an emergency drainage system are arranged in a closed water circulation culture system, wherein the culture water circulates in different culture and water processing units. As the water circulates in different culture units and is utilized in multiple levels by various aquatic organisms of different trophic level and ecological niche, the system gives the various aquatic organisms full play to the positive bait resource complementarity, prevents the aquatic organisms from directly competing on living space and dissolved oxygen and reduces the mutual damage caused by organic remains and metabolic waste, achieves the 'self-modification' and 'biomanipulation' of the culture environment, enables the environment itself to recover from the pollution in culture process, and decontaminates the seawater culture environment.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of mouse embryonic fibroblast feeder cells to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by defined components added to the culture environment that support rapid proliferation without differentiation. The medium contains an isotonic buffer, a blend of essential nutrients such as protein and lipids, and an effective growth factor or combination of factors that promote proliferation while inhibiting differentiation. Culturing human embryonic stem cells in fresh medium on an extracellular matrix according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

The present invention provides a biological sample culturing and observation system which includes: an incubator in which a biological samples are housed, and whose interior is maintained in a culturing environment satisfying predetermined conditions, and the interior is isolated from the outside; an observation optical system that from outside the incubator optically observes the biological sample via the incubator; a light blocking device that blocks external light that is irradiated on the biological sample and within the visual field of a observation optical system; and a supply device that selectively supplies a liquid or gas to the biological sample inside the incubator from a plurality of holding vessels that individually hold a plurality of different types of liquid or gas that are necessary for the culturing.

The invention relates to a pig feed additive capable of replacing antibiotics, and a preparation method and an application thereof. Components and weight part ratio thereof in a formula are as follows: 1-5 parts of medicated leaven, 1-5 parts of angelica sinensis, 1-5 parts of radix astragali, 1-5 parts of hawthorn, 1-5 parts of radix codonopsis, 10-20 parts of citric acid, 10-20 parts of lactic acid, 20-30 parts of coated phosphoric acid, 5-10 parts of a micro-ecological preparation, 5-10 parts of yeast cell wall polysaccharide, 1-5 parts of a composite plant essential oil and 5-10 parts of a carrier. The pig feed additive carries out combination collocation of traditional Chinese herbs and functional polysaccharide, the micro-ecological preparation, acidifying agents, plant essential oil and the like, so that synergic effect of the traditional Chinese herbs and other components are maximized; the pig feed additive can replace the antibiotics completely; problems of drug resistance, drug residues, overcommitment and the like of the antibiotics which trouble animal husbandry for a long time is reduced; and at the same time, the pig feed additive plays effects of improving culture environment, reducing feed safety hidden dangers, increasing culture benefits and the like.

The invention discloses a green biological active feed, which relates to the technical field of feeds. The green biological active feed is prepared from the following materials in part by weight: 40 to 80 parts of straw, 5 to 30 parts of corn four, 5 to 25 parts of cake dregs, 5 to 25 parts of wheat bran, 0.1 to 0.2 part of complex bacteria and 0.1 to 0.2 part of complex enzyme. During preparation, the straw is crushed and then is uniformly mixed with the corn flour, the cake dregs and the wheat bran; the complex bacteria and the complex enzyme are mixed into the mixture; water is added into the mixture to ensure that the water content reaches 45 to 50 percent, and the mixture is filled in a big tank, sealed and stored for later use; or the mixture is baked or dried by natural wind for storage and later use. The green biological active feed has the following advantages of: (1) improving the balance of intestinal flora, and improving the feed utilization rate; (2) promoting the growth of meat livestock and poultry, and shortening the breeding cycle; (3) strengthening the body conditions of laying hens, ducks and geese, and having a significant strengthening action on prolonging theegg-laying peak period; (4) strengthening the body condition of female domestic animals, and improving the yielding rate; (5) strengthening the body conditions of the livestock and poultry, enhancingthe disease prevention and disease resisting capacities, and reducing the sickness rate; and (6) purifying the culture environment, and improving the meat quality due to no public nuisances and no residues.

The invention discloses a fowl manure fermentation fertilizer. The fertilizer is produced by materials of fresh fowl manure, rice bran powder, maize meal, wheat flour, brown sugar, lime powder, dinoflagellate, microzyme, lactobacillus, thermophilic actinomycetes, etc. The invention improves culture environment of henhouses, and heavy metal in the fowl manure is degraded and changed into treasure, becoming an ecological organic fertilizer which is comprehensively and abundantly nutrient. Nitrate and secondary salt formed in the soil by fertilizer residual can be degraded by the fowl manure fermentation fertilizer, and therefore functions of disease resistant, lodging resistant, crop yields improvement can be realized; ventilation property of the soil is improved, soil hardening and virus can be reduced and banished, meanwhile, dosage of the chemical fertilizer and pesticide is reduced; production cost is reduced, pollution is reduced and soil environment is optimized, and microeubiosis of the soil is achieved; therefore the crops can be ripen in advance, grade of the crops is improved, hazardous and noxious substance contents remained in the crops are lowered and banished, and the green environmental requirements of heavy metal residual of the crops can be achieved.

This disclosure provides an improved system for culturing human embryonic stem cells. The cells are cultured in suspension so as to maximize the production capacity of the culture environment. The new culture system of this invention allows for bulk proliferation of hES cells in a more cost-effective manner, which facilitates commercial production of important products for use in human therapy.

The invention discloses a livestock culture environment regulating device, comprising a measurement and control unit which comprises an intelligent measurement and control terminal. The intelligent measurement and control terminal comprises a microprocessor, a detector connected with the microprocessor and a control output which is connected with a control device; the detector comprises a temperature and humidity detector and an ammonia concentration detector; the control device comprises an air blower; the livestock culture environment regulating device is characterized in that the detector also comprises dust concentration monitoring, illuminance detection and wind speed detection; the control device also comprises a lighting device, an atomization device, a heating device and a sprayingdevice; when temperature reduction is required, the device performs temperature drop by adopting the mode of combining the air blower with atomization, namely, when the temperature is higher than theset value, temperature drop is carried out by adopting the mode of combining atomization with the air blower; and when the temperature is lower than the set temperature, temperature drop is carried out by adopting the air blower. The invention has the advantages of dropping temperature by combining the air blower with atomization, being capable of multifunctional detection and fuzzy control and the like.

A microecological water quality conditioning agent applicable to restoring of sea cucumber culture water body and a method for preparing the microecological water quality conditioning agent belong to the technical field of aquaculture and microorganisms. By aiming at physiological habits and culturing characteristics of sea cucumbers and applying modern biological technique, the microecological water quality conditioning agent is made of bacillus flexus CGMCC (China general microbiological culture collection center) No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117, which are separated and sieved from a sea cucumber culturing environment and prepared by steps: using corn starch and soybean cake powder as main culturing materials, fermentating for 24-48h at 25-40 DEG C, and using zeolite and wheat bran as adsorption carriers for processing to obtain the microecological water quality conditioning agent. The microecological water quality conditioning agent is less susceptible to environment conditions, has remarkable purifying effect on sea cucumber culturing water body, and is capable of greatly reducing content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, deodorizing and effectively restraining pathogenic bacteria so as to prevent sea cucumber diseases and improve yield and quality of cultured sea cucumbers.

The invention discloses a multi-ecological niche bioremediation method of seawater aquiculture environment, which comprises the steps of draining aquiculture wastewater into an aquiculture trench, adding beneficial bacteria to remove nitrogen and phosphorous and convert organic matters on the first stage, culturing filter-feeding fishes and large-scale seaweed to form a multi-ecological niche biosphere coexisting microalgae, filter-feeding fishes and large-scale seaweed in the second stage, and allowing bioremediation of seawater aquiculture environment by utilizing different ecological niches of beneficial bacteria, microalgae, filter-feeding fishes and large-scale seaweed through synergic action of organisms. The inventive method can clean aquiculture wastewater to meet discharge standards, remediate aquiculture environment, and obtain fishes and large-scale seaweed, thereby bringing environmental, economic and ecological benefits.

The invention discloses a method for producing Anrodia camphorata mycelia based on solid-state surface culture, and belongs to the field of bioengineering. The method comprises the following steps of: screening components of a culture medium of the Anrodia camphorate and optimizing the proportion of the cereal raw material in the culture medium so that the culture medium is firmly adsorbed to the inner wall of a culture vessel or the culture surface to bring convenience to the surface culture of the Anrodia camphorata mycelia; inoculating the Anrodia camphorata mycelia in the culture surface of a reactor and culturing the Anrodia camphorata mycelia in the environment of certain temperature and moisture; feeding the culture medium every a certain time in batch during the surface culture so as to timely replenish the nutrient components required by the growth of the Anrodia camphorata mycelia and making the growth environment of the Anrodia camphorata mycelia close to the natural culture environment until a large amount of mycelia or large colony is formed; and quickly drying the Anrodia camphorata culture at a low temperature after the culture. The method of the invention makes the growth environment of the Anrodia camphorata mycelia close to that of the wild Anrodia camphorate and improves the productivity of the Anrodia camphorata culture by timely feeding the culture medium in batch, so that the obtained Anrodia camphorata culture has high content of active ingredient and closer efficacy to the wild fruit body.

The invention relates to a feed additive premix for low-salinity cultured marine fishes. The feed additive premix for the low-salinity cultured marine fishes consists of multi-vitamins and composite trace elements, and 20 to 30kg of feed additive premix is added into each ton of marine fish feed, wherein the multi-vitamins are 5 to 10kg and the composite trace elements are 15 to 25kg. Long-term tests of the low-salinity cultured marine fishes in the Pearl River Delta, such as trachinotus ovatus, Japanese sea bass, sciaenops ocellatus, sparus latus, sparus macrocephalus, latescalcarfer and the like, prove that the feed additive premix for the low-salinity cultured marine fishes has comprehensive nutrition and can meet the requirement of various marine fishes on total nutrition in a low-salinity culture environment; the feed additive premix is safe and high-efficiency, does not have any toxic or side effect, makes cultured objects grow rapidly and has high feed conversion rate and good culture benefit if taken for a long time; meanwhile, the additive can obviously enhance the immunologic function and anti-stress capability of the cultured objects, and the cultured marine fishes have high vigor, bright body color and high survival rate.

The invention discloses a strong lysis bacteriophage for splitting NDM-1 positive Klebsiella pneumoniae clinical isolates, and an application of the strong lysis bacteriophage used as a biological preparation in the prevention and treatment of NDM-1 Klebsiella pneumoniae induced infection and pollution. The preservation number of the bacteriophage is CCTCC M 2015760, and the bacteriophage has a regularly icosahedral head and a telescopic tail, and belongs to a Myoviridae double-stranded DNA bacteriophage. The bacteriophage has strong kilign activity and wide bacteriophage lysis spectrum, can be used for preparing medicines for preventing and treating NDM-1 Klebsiella pneumoniae induced bacterium infection, and also can be used for killing NDM-1 positive Klebsiella pneumoniae in culture environment and medical environment.

The invention relates to a greenhouse wireless sensor network control node device for intensive aquiculture by using a wireless measurement and control method, which is installed in a wireless measurement and control network of intensive aquiculture. The device is arranged in a culturing farm of an intensive aquiculture region, a collection node is used for collecting the parameters of water quality environment sensors, and the data collected by the sensor group is preprocessed by combining a rule base and an information fusion algorithm. A radial basis function neutral network algorithm (RBF algorithm) and the fuzzy computing technology are adopted as an algorithm model of information fusion, the collected data is subject to field level data fusion, and a ZigBee wireless module is used for sending fused abnormal water quality environment state and environment parameter data to a convergent node. The convergent node uploads the data to a monitoring center, and the monitoring center can monitor the growth conditions of cultured organisms, environmental conditions, control device operating conditions and the like of a plurality of culturing farms within a monitoring range in real time. The collection node can also receive the feedback information of the convergent node and the monitoring center and adjusts the parameters of the information fusion algorithm. The device is an information collection device for a wireless sensor network, has high efficiency, reliability and convenient operation, and is used for solving the difficulties of data real-time collection for culturing environment and filed level data fusion in the intensive aquiculture process by using the wireless sensors.

The invention discloses a fermented feed. The fermented feed is prepared by the following steps of: performing amplification culture on Lactobacillus acidophilus ACCC10637 and Bacillus subtilis ACCC10619 respectively to obtain amplification culture solution by taking soybean whey as a raw material of a liquid culture medium, and performing amplification culture on Candida utilis ACCC20060 to obtain an amplification culture medium by a semi-solid culture medium; and according to the weight of a semi-solid-state fermentation culture medium, adding 3.2 percent of amplification culture solution of the Lactobacillus acidophilus ACCC10637, 1.0 percent of amplification culture medium of the Candida utilis ACCC20060, and 0.1 percent of amplification culture solution of the Bacillus subtilis ACCC10619 into the semi-solid-state fermentation culture medium respectively, and sealing and fermenting for 36 to 72 hours at the temperature of between 28 and 32 DEG C. When 10 percent of fermented feed is added into a pig feed, the utilization rate of the feed can be improved by 7 to 8 percent, the morbidity is reduced by 8 to 10 percent, and the culture environment is remarkably improved.

The invention relates to the technical field of edible and medicinal fungus culture, in particular to a wild imitating culture collection method for lucid ganoderma and lucid ganoderma conidial powder. The method comprises eight work procedure steps including (1) strain selection; (2) culture environment preparation; (3) culture material preparation; (4) bag sheathing and sterilization; (5) inoculation and fungus growing; (6) land preparation and soil covering; (7) lucid ganoderma growth and culture; and (8) powder collection through sleeve barrels. The wild imitating culture collection method for lucid ganoderma and lucid ganoderma conidial powder provided by the invention has the advantages that through the full improvement on the work procedure steps, the completely imitated wild lucid ganoderma survival environment and internal hereditary basis as well as external substance basis are provided for culturing and collecting high-yield and high-quality lucid ganoderma conidial powder, the cultured and collected lucid ganoderma conidial powder has high yield and high quality, the grains are full, and the purity is as high as more than 99.9 percent.

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

The invention provides an industrial loach breeding and fry culturing method and belongs to the field of aquaculture. The method includes industrial breeding and fry culturing workshop construction and a specific breeding and fry culturing method. The specific breeding and fry culturing method includes: arranging bi-layer open net cages in a cement pool in a breeding workshop, wherein inner net cages are rutting and spawning places for parents, and outer net cages receive eggs concentratedly; and arranging a plurality of open foam boxes in a hatching and fry culturing workshop, placing the eggs into the foam boxes after counting, feeding loach fries by micro initial feed after hatching to fries to enable the loach fries to live through an initial critical period to become strong fries, and selling the fries or placing the fries into a pond for large-scale fingerling rearing. A workshop production process is adopted, breeding and hatching and fry culturing environments are guaranteed effectively, operation is facilitated, working efficiency is high, fecundity can be measured accurately, fertilization rate reaches above 98%, hatching rate basically reaches 100%, fry survival rate basically reaches 100%, and survival rate of large-scale fingerling rearing by placing the strong fries into the pond reaches above 50%.

The invention relates to the aquaculture field and provides a method for culturing cowfishes by using a net cage. The net cage comprises a metal frame and two paratactic cage bodies formed by independent nets in an enclosing way. The cage bodies are connected through a pedestrian road. The frame is provided with a floating device. Sinkers are arranged at the four corners of the bottom of each cage body. Fixing devices are arranged around the net cage. The method comprises the following steps of: adopting a method matching voice driving with seining to catch the cowfishes; adopting a semidry transportation method to deliver the cowfishes; adopting a field temporary culture and artificial feeding method to domesticate the cowfishes to adapt to the culture environment of the net cage; and conducting net cage culture and daily cleaning management. The method can resist the influences of water flows, tides, waves and the like, can maintain the shape retentivity and the setting stability ofthe culture net cage and can help the cowfishes grow and develop.

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

The invention discloses biological enzyme protein feed additives which comprises microbe live bacteria cells, active biological enzymes, amino acid, starch, fibers and water. The microbe live bacteria are Candida utilis bacteria, lactobacillus acidophilus and Bacillus licheniformis. The active biological enzymes are protease, amylase, beta-glucanase, xylanase, pectase, glucoamylase and fat acid enzyme, and amount of the active biological enzymes is 20000-24000 mu / g. The additives are full of microbe live bacterial and active biological enzymes for growth, metabolism, digestion, absorption, immunoregulation of livestock and poultry, intestinal tract flora balance capability, intestine mucous membrane epithelial cells restoration capability, intestinal villus growth capability, vitamin and trace element supply capability of the livestock and poultry are maintained. Absorption and utilization rate of the feed additives is high. According to the feed additives, drain amount of nitrogen, phosphor and calcium in night soil of the livestock and poultry is reduced, qualities of livestock, poultry and aquatic products are raised, and environmental pollution is reduced. Offensive odor of the livestock and poultry night soil is weakened and culture environment is purified.

The invention relates to a Salmonella bacteriophage, particularly a wide-host-spectrum strongly-lytic Salmonella pullorum bacteriophage. The invention also relates to Salmonella pullorum for controlling the bacteriophage in a culture environment. The Salmonella pullorum bacteriophage is named PSPu-4-116, and collected in China Center for Type Culture Collection, and the collection number is CCTCC M2011042.

The invention provides a method for producing rabies viruses by the suspension culture of BHK21 cells, which comprises a step of performing suspension culture of BHK21 cells in bioreactor containing a BHK21 cell culture medium, wherein the conditions for the suspension culture in the step include a temperature of 32 to 36 DEG C, a pH value of 7.0 to 8.0 and a dissolved oxygen concentration of 30 to 50 percent; and the BHK21 cell culture medium comprises the components shown in a table 1. The method overcomes the biases of the prior art and realizes the production of the rabies viruses by the suspension culture of BHK21 cells in the bioreactor. The obtained rabies viruses can be used for producing rabies vaccine. Due to the automatic culture environment parameter control of the bioreactor, the cells grow and the viruses propagate in more favorable environments, the virus titer is improved and the large-scale automatic continuous production can be realized.

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The invention relates to a coliphage and application thereof, in particular to a short-tailed highly-lytic coliphage. The coliphage named PD38 is collected in the China General Microbiological CultureCollection Center on September 26, 2018, wherein the collection number is CGMCC No. 16391. The coliphage has a good lytic effect on escherichia coli, has a good killing effect on the escherichia coliin food and culture environment, and can prevent and treat diseases caused by the escherichia coli of chicken.

The invention relates to a root acclimation method of a camellia from soil culture to water culture. The core of the invention is characterized in that: the camellia is put in a water culture environment which is oxygenized timely for root acclimation in earlier stage of water culture till the camellia under acclimation send up water roots which adapt to the water culture environment, and therefore, the camellia that is cultured in soil is finally acclimated to completely adapt to the water culture environment to grow normally. The root acclimation method of the camellia has little stimulation to the camellia, and avoids a second adaptation process of the camellia after acclimation in clear water and then being transplanted in a nutrient solution environment, and can save labor force and cost.