318 results about "K pneumoniae" patented technology

The invention relates to a yarn, especially the manufacturing method for blended yarn of chitin fiber, alginate fiber and tencel fiber, and the application. The blended yarn comprises 5-30 by wt of chitin fiber, 5-40 by wt of alginate fiber and 30-70 by wt of tencel fiber. Merits of invention are that (1) produced blended yarn and the product is environmental protection and degradation material; (2) produced blended yarn and the product is washing resistance and long time for medical and health efficacy; (3) the product is provided with the health action of bacteriostasis, diminishing inflammation, deodorization and so on, and the antibacterial ratio for staphylococcus aureus and Klebsiella pneumoniae bacterium can achieve the standard of national ministry of health; (4) the product is provided with wide application, it can be made into knitting, woven and non-woven fabrics; it can not only be applied in textile for medical treatment but also for ordinary clothes.

The invention discloses a strong lysis bacteriophage for splitting NDM-1 positive Klebsiella pneumoniae clinical isolates, and an application of the strong lysis bacteriophage used as a biological preparation in the prevention and treatment of NDM-1 Klebsiella pneumoniae induced infection and pollution. The preservation number of the bacteriophage is CCTCC M 2015760, and the bacteriophage has a regularly icosahedral head and a telescopic tail, and belongs to a Myoviridae double-stranded DNA bacteriophage. The bacteriophage has strong kilign activity and wide bacteriophage lysis spectrum, can be used for preparing medicines for preventing and treating NDM-1 Klebsiella pneumoniae induced bacterium infection, and also can be used for killing NDM-1 positive Klebsiella pneumoniae in culture environment and medical environment.

The invention relates to a multifunctional biological seed coating agent and a preparation method thereof. The multifunctional biological seed coating agent comprises auxiliary agents and beneficial microorganism, volume ratio thereof is 1:1-5, and effective bacterium content is not less than or equal to 10<8>cfu / ml. The auxiliary agent comprises one or various auxiliary agents in film former, dispersant, adhesion agent, protectant, nutritient, anti-freeze agent, preservative and colourant. The beneficial microorganism is selected from one or various rhizobium, azotobacter, pseudomonad, bacillus, K pneumoniae, Enterobacter cloacae and Raoulterlla planticola. The preparation method comprises the following steps: dissolving the polyvinyl alcohol and natural substance with water in a reaction kettle, mixing with the water dispersion liquid of the adhesion agent to prepare a film forming agent, and finally mixing and stirring mechanically with the beneficial microorganism fermentation liquor to obtain the finished product. The invention has the advantages of no residue, high efficiency, low toxicity, strong specificity, good film forming effect and convenient application, therefore, the multifunctional biological seed coating agent plays an significant role of preventing and controlling cotton seedling diseases as well as enhancing the cotton seedling growth under salt stress.

The invention discloses polyurethane synthetic leather capable of inhibiting bacteria and resisting mildew and a preparation method thereof. The polyurethane synthetic leather capable of inhibiting bacteria and resisting mildew comprises a polyurethane resin and a composite antiseptic uniformly dispersed in polyurethane, wherein, the composite antiseptic comprises 0.01 to 20 parts of an inorganic antiseptic, 0.01 to 35 parts of an organic antiseptic, 0.0001 to 0.4 part of a compatilizer and 0.0001 to 0.4 part of an anti-blushing agent. The polyurethane synthetic leather provided in the invention has instant, high efficiency and lasting functions of inhibiting bacteria and resisting mildew; according to results of antibacterial property detection, the antibacterial polyurethane synthetic leather has good inhibition and killing effects on staphylococcus aureus, Klebsiella pneumoniae, escherichia coli, mixed mycete, etc., an antibacterial rate is greater than 99%, and a mildew-resistant grade is up to 1-0 grade. The preparation method comprises the following steps: dispersing the composite antiseptic with dimethyl formamide (a DMF solvent), adjusting viscosity of an obtained mixture until the viscosity of the mixture is close to the viscosity of molding slurry of the polyurethane synthetic leather, uniformly mixing the mixture with the molding slurry of the polyurethane synthetic leather and carrying out molding so as to prepare the polyurethane synthetic leather capable of inhibiting bacteria and resisting mildew. The method is simple and reliable, does not change an original process route and enables production cost for a product to be controlled.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam lysinate delivered as an aerosol or dry powder formulation.

The invention provides a klebsiella pneumoniae detection kit. The klebsiella pneumoniae detection kit comprises two pairs of primers, i.e., an inner primer FIP / BIP and an outer primer F3 / B3, which use oppA gene of klebsiella pneumoniae as the target gene and is designed based on the loop-mediated constant-temperature amplification technology. The klebsiella pneumoniae detection kit has more comprehensive detection effect and low omission ratio.

The present invention provides a gene chip for detecting common pathogen in dairy, which comprises a solid-phase vector and an oligonucleotide probe fixed on the solid-phase vector, wherein the oligonucleotide probe includes DNA fragment or its complementary DNA or RNA sequence selected from E. sakazakii, streptococcus pyogenes, staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Listeria monocytogenes, salmonella and 16S-23S rDNA intergenic spacer region of Bacillus cereus, as well as ipaH gene of Shigella or gyrB gene of citrobacter freundii. The invention further provides a reagent box which uses the gene chip to detect pathogen in dairy. The gene chip and the reagent box of the invention for detecting pathogen in dairy are easy to operate, highly precise and greatly repeatable.

The invention provides a gene chip of main pathogenic microorganism in drinking water and a testing kit, which mainly aims at 11 kinds of bacteria of colibacillus / Shigella, salmonella, vibrio cholera, vibrio parahaemolyticus, staphylococcus aureus, enterococcus faecails, pseudomonas aeruginosa, legionella pneumophilia, pneumobacillus, yersinia enterocolitica and the like, and L.interrogans. The gene chip comprises a solid phase carrier and a oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe contains gyrB gene with tremendous evolutionary advantage, ITS gene and DNA segment selected from 16srRNA gene or complementary DNA segment. The gene chip and the testing kit of the invention can test the main pathogenic microorganism in drinking water, and has the characteristics of simple operation, high throughput, high accuracy, strong repeatability and the like, and can be used for clinical test for the water quality monitoring department.

The present invention discloses a composite microbial fertilizer. It is characterized by utilizing three bacterial powders of Bacillus mucilaginosus CGMCC, No.1641, Klebsiella pneumoniae subsp pneumonaiae CGMCC, No.1642 and Bacillus megaterium CGMCC, No.1640, mixing them according to weight ratio of 1:1:1 to obtain their mixture and mixing said mixture with a synergistic agent according to a certain mixing ratio so as to obtain the invented microbial fertilizer.

A biological inoculant for enhancing the growth of plants is disclosed. The inoculant includes the bacterial strains Herbaspirillum seropedicae 2A, Pantoea agglomerans P101, Pantoea agglomerans P102, Klebsiella pneumoniae 342, Klebsiella pneumoniae zmvsy, Herbaspirillum seropedicae Z152, Gluconacetobacter diazotrophicus PA15, with or without a carrier. The inoculant also includes strains of the bacterium Pantoea agglomerans and K. pneumoniae which are able to enhance the growth of cereal grasses. Also disclosed are the novel bacterial strains Herbaspirillum seropedicae 2A, Pantoea agglomerans P101 and P102, and Klebsiella pneumoniae 342 and zmvsy.

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

The invention discloses a kit for detecting klebsiella pneumoniae, and belongs to the technical field of PCR detection. The kit comprises specific primers and a probe for detecting klebsiella pneumoniae; the nucleotide sequences of the specific primers and the probe are as shown in SEQ ID NO.1-3; a detected target gene is a sequence of a pho gene, and has a nucleotide sequence as shown in SEQ ID No.4. The kit has the advantages of being accurate in detection, high in sensitivity, strong in specificity, and simple and quick to operate, has a favorable sample detection capacity, can replace a traditional bacterial isolated cultivation and diagnosis method, and the novel kit for quick detection is provided for klebsiella pneumoniae.

The bamboo forest biolgoical fertilizer capable of meeting the requirements for biological organic fertilizer in production of nuisance-free green bamboo shoots and organic bamboo shoots for reducingor substituting chemical fertilizer is formed from matrix and bamboo plant rhizospheric combined nitrogen-fixing bacteria which are separated from bamboo plant rhizosphere, can be used for fixing molecular nitrogen in atmosphere and are any one strain or combination of any two kinds of strains or more than two kinds of (Bacillus polymyxa mace) WG-1 strain, (Bacillus polymyxa mace) WG-2 strain, (Bacillus licheniformis Chester) WG-3 strain and (Klebsiella pneumoniae (Schroeter) Trevisan) WG-4 strain. Said fertilizer can be used for cultivating bamboo forest.

The invention discloses new klebsiella pneumoniae phage vB_KpnM_Bp5. The klebsiella pneumoniae phage (klebsiella pneumoniae phage) phage ) vB_KpnM_Bp5 is preserved in the China Center for Type CultureCollection (CCTCC) on June 13, 2019, and the preservation number is CCTCC NO:M 2019452. The phage has better disinfecting and sterilizing effects on host multidrug resistant klebsiella pneumoniae separated from piggery sewage, also has good disinfecting and sterilizing effects on other two multidrug resistant klebsiella pneumoniae separated from hospitals, is wide in antimicrobial spectrum, and can be applied to preparation of medicines for preventing and treating multidrug resistant klebsiella pneumoniae.

The invention discloses a method for producing 2,3-butanediol by using starch raw materials, which comprises the following steps: subjecting the starch raw materials to treatment by alpha-amylase and maltogenic amylase; and producing the 2,3-butanediol by carrying out separate hydrolysis and fermentation or simultaneous sacchrification and fermentation under an aseptic condition, using saccharification liquid or liquefaction liquid as a fermentable substrate and using Klesiella pneumoniae as a fermentable strain. Finally the concentration of the 2,3-butanediol reaches 43 to 112g / L, and the final concentration of acetoin and the final concentration of the 2,3-butanediol reach 45 to 121g / L. The method has the advantages of using the starch raw materials to produce a high-value platform compound by fermentation, reducing the raw material cost of the production of the 2,3-butanediol by the fermentation and establishing the optimal fermentation condition for the production of the 2,3-butanediol by the sacchrification and fermentation of the starch raw materials. The production of the 2,3-butanediol by the simultaneous sacchrification and fermentation of the invention can save energy and equipment investment, reduce fermentation period and improve production efficiency.

The invention discloses a primer and a probe for quantitative determination of klebsiella pneumonia, and an application of the primer and the probe. Nucleotide sequences of the primer are a sequence 3 and a sequence 4 in a sequence table; a nucleotide sequence of the probe is a sequence 5 in the sequence table. The primer and the probe have the beneficial effects that the specific primer and probe sequences of the klebsiella pneumonia disclosed by the invention can be applied to qualitative and quantitative detection of the klebsiella pneumonia, the target of accurately and quantitatively determining the deoxyribonucleic acid (DNA) content of the klebsiella pneumonia in a specimen to be detected can be achieved by extracting the DNA in the sample to be detected and combining with a real-time fluorescence quantification polymerase chain reaction (PCR) detection technology, and the primer and the probe can be applied to food detection, scientific research and clinical diagnosis and used for carrying out qualitative and quantitative analysis on the klebsiella pneumonia DNA in samples such as a food sample, a suffer throat swab, nasopharyngeal secretion, people sputum specimens and blood samples. Thus, the primer and the probe have an important significance on judgment of klebsiella pneumonia infection, evaluation of treatment effectiveness and dynamic observation of the state of an illness, and simultaneously play an important role in the field of detection of clinical medicine.

The invention provides a fluorescent detection kit and a detection method for Klebsiellapneumoniae carbapenamase. The sequences of specific primers and a fluorescent probe in the kit are as follows: a forward primer: 5'-ACT GGG CGC GCACCTA-3'; a reverse primer: 5'-TCG CTGTGC TTG TCATCC TT-3'; and the fluorescent probe: 5'-FAM-CCG TCT ACACCCGGG CGC C-TAMRA-3', wherein the FAM is a fluorescent reporter, and the TAMRA is a fluorescent quencher. The invention has the advantages that: the detection kit has high sensitivity and good specificity for the Klebsiellapneumoniae carbapenamase, and reduces the false positive rate of the conventional polymerase chain reaction (PCR) amplification; and quick, accurate and specific detection for the Klebsiellapneumoniae carbapenamase can be realized.

The invention provides a gene chip for detecting pathogens of lower respiratory tract, which comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises a sequence selected from one or more DNA sequences of staphylococcus aureus, 16s-23s rDNA intergenic spacer region of Klebsiella pneumoniae or Acinetobacter baumannii, 16S gene of pseudomonas aeruginosa or haemophilus influenzae, gyrB gene of streptococcus pneumoniae or legionella pneumophila and copB gene of moraxella catarrhalis or the complementary DNA or RNA sequence. The invention further provides a reagent kit containing the gene chip. The utilization of the gene chip and the reagent kit can detect the pathogens of the lower respiratory tract; furthermore, the operation is simple, the accuracy is high and the repeatability is strong.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

The invention relates to a group of insect Thanatin ramifications with antibacterial action and the salt which is applicable for medicinal use, as well as a preparation method and the use thereof. Approved by the results of a sterilization experiment, a hemolysis experiment and an irritable partial stimulation experiment, the insect Thanatin ramification has bigger antibacterial activity than Thanatin to colon bacillus, Candida albicans, Klebsiella pneumoniae, and staphylococcus aureus. The hemolysis reaction is not incurred when the concentration is 2.5mg / ml without acute toxicity stimulation reaction.

The invention discloses a Klebsiella pneumoniae SDM CCTCC M 208097 and applications in preparing 2, 3-butanediol. The Klebsiella pneumoniae SDM CCTCC M 208097 has features of wide nutritional requirement range, high outcome concentration and short production period or the like. The culture medium and the operation method are simple, the cost is low. The outcome of the 2, 3-butanediol prepared by a fermenter feeding batch fermentation can reach 120-160g / L, the conversion can reach 90-940f the theoretic conversion, the most production rate of the 2, 3-butanediol is 4. 05g / (L. h). The invention has very large commercial use prospect.

The invention discloses a bacteriophage depolymerase capable of degrading Klebsiella pneumoniae capsular polysaccharides and biofilms. By prokaryotic expression and purification of a 32nd open readingframe in a genome of Klebsiella pneumoniae bacteriophage GH-K3, a brand-new depolymerase depo32 with high activity is obtained. The depolymerase has high-efficiency degradation effect on the capsularpolysaccharides and biofilms formed by Klebsiella pneumoniae.

The invention relates to a long-acting antibacterial amino molding compound. A silver nanowire composite antibacterial assistant comprises the following components in parts by weight: 0-50 (excluding 0) parts of silver nanowire, 0-25 (excluding 0) parts of nano zinc compound, 10-100 parts of alcohol compound, 10-100 parts of distilled water and 0.1-5 parts of hexamethylenetetramine. The long-acting antibacterial amino molding compound comprises the following components in parts by weight: 2000-4000 parts of urea, 4000-8000 parts of formaldehyde, 200-400 parts of melamine, 10-150 parts of silver nanowire composite assistant, 50-150 parts of hexamethylenetetramine, 20-50 parts of ammonium sulfamate curing agent, 100-500 parts of filler, 1000-2500 parts of cellulose, 20-100 parts of lubricant and 1-100 parts of dispersing agent. The antimicrobial ratio of the molding product of the long-acting amino molding compound for Escherichia coli, Staphylococcus aureus, Friedlander's bacillus, Salmonella typhimurium and Enterococcus faecalis is greater than 99.9%, the antimicrobial activity value is greater than 4, and the long-acting amino molding compound has the advantages of favorable mechanical properties and favorable moldability.

The invention discloses a PCR (Polymerase Chain Reaction) amplification primer for quickly detecting klebsiella pneumonia and application thereof, and belongs to the field of animal bacteriology and molecular biology. The PCR amplification primer is prepared from an upstream primer having a nucleotide sequence as shown in SEQ ID NO:1 and a downstream primer having a nucleotide sequence as shown inSEQ ID NO:2; the PCR amplification primer is used for preparing a PCR amplification kit for detecting the klebsiella pneumonia. A PCR detecting method provided by the PCR amplification kit has high specificity and sensitivity, good repeatability and high credibility and is capable of specifically detecting the klebsiella pneumonia and quickly and accurately obtaining a detecting result; meanwhile, the PCR amplification kit is low in cost and simple in operation, is suitable for being used at the primary level and can be used as a quick, accurate and simple detecting tool for quick laboratoryidentification of the klebsiella pneumonia and large-scale epidemiological investigation.

The invention discloses a novel klebsiella pneumoniae strain as well as an isolation method and application thereof. The novel strain is isolated and screened from activated sludge in an aeration tankof a papermaking sewage treatment plant and is collected in China General Microbiological Culture Collection Center, the collection name is ZS-01, the collection number is 16041, and the collection date is June 30, 2018. The strain has the characteristics and performance as follows: (1), the characteristics are: colonies formed on a phenol MedA solid culture medium are relatively short and thickbacilli, have the sizes of (0.5-0.8)*(1-2)[mu]m, are arranged separately or in pairs, and are pale yellow, uniform in texture and opaque; relatively large gray white sticky colonies are formed on an MPYE solid culture medium, are flagella-free, have capsules, belong to enterobacteriaceae and are Gram-negative short and thick bacilli; (2), the performance is: the strain has certain phenol degradingcapability and has relatively high tolerance to phenol. The strain has the advantages as follows: the strain can degrade the phenol with relatively high concentration, and provides a new bacterial source for effectively treating phenol-containing wastewater with relatively high concentration.

A newly isolated lytic bacteriophage specifically against Klebsiella pneumoniae, deposited at DSMZ-Deutsche Sammlung von Mikroorganismen and Zellkulturen with deposit number (DSM 24329), shows broad host ranges and lytic effects on K. pneumoniae isolated from Taiwan under either in vivo or in vitro models.

The invention relates to Klebsiella pneumoniae and a method of preparing hydrogen and 2, 3-butanediol thereby. The Klebsiella pneumoniae is Klebsiella pneumoniae ECU-21 with the preservation number of CGMCC No. 2850. In the preparation method, a ring colony taken from a panel is inoculated to a seed culture medium, the inoculated seed culture medium is arranged in an incubator of 37 DEG C for the static culture, and the seed culture medium which is cultured for 12 hours is switched to a fermentation medium for the fermentation culture; after the inoculation, a rubber stopper is used for sealing a fermentation shake flask, the anaerobic culture environment is formed by introduced N2 sweeping, superfluous gas is discharged via an exhaust port, a gas bag is used to be connected with the exhaust port for collecting hydrogen produced during the fermentation after the gas discharge is finished, and the 2, 3-butanediol is obtained in liquid product; the culturing temperature is 37 DEG C, a magnetic stirrer is adopted for stirring, the rotation speed is 150rpm, and the culturing time is 12-15h. The invention has the advantages that the hydrogen and the 2, 3-butanediol are produced by the fermentation under the anaerobic condition, the additional value of the production is increased, the reaction conditions are easy and wide industrial application development prospect is provided.

The invention discloses a longifolene derivative (2, 2, 8, 8-tetramethyl-4-atrane [5. 4. 0. 11, 9] dodecane-6-alkene obtained by rearrangement and reduction reaction and by taking isolongifolene ketoxime as a raw material. Besides, bacteria such as staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, bacillus proteus vulgaris and pseudomonas aeruginosa can be inhibited by the longifolene derivative, and particularly, the longifolene derivative has a superior insecticidal effect on pests such as aphids and rice planthopper.