The invention discloses a method for extracting umbilical cord mesenchymal stem cells by combining grinding with mixed enzyme. The method comprises the steps of 1, removing both ends of the umbilicalcord, and washing arteriovenous blood stains of the umbilical cord; 2, removing umbilical arteries and umbilical veins, cutting the umbilical cord into small pieces, peeling off an adhesive layer, cutting it into smaller pieces; 3, splitting and charging the tissue pieces into a centrifuging tube, and grinding the tissue into meat paste with a tissue grinder; 4, mixing collagenase and trypsin witha meat paste suspension and shaking the mixture in a shaker; 5, after neutralizing with a culture medium, centrifuging supernatant, mixing with a culture solution is executed, and adding the mixtureinto a cell culture flask; 6, standing and culture, digesting with enzyme, and then executing subculture; 7, continuing to culture for a period of time, subculturing P1 generation and cryopreserving the P2 generation. Based on the method of combining tissue grinding with digestion, the yield of primary cells can be greatly improved, and cell damage can be decreased.