The invention relates to a recombinant heat-resistant DNA polymerase. The recombinant heat-resistant DNA polymerase comprises a DNA binding structural domain, a protein structural domain connector and a heat-resistant DNA polymerase without 3'-5' nucleic acid exonuclease activity, wherein the DNA binding structural domain is a Sso7d structural domain, HMf protein, or an HhH structural domain of DNA topoisomerase V from methanothermus fervidus; the heat-resistant DNA polymerase without the 3'-5' nucleic acid exonuclease activity is a deletion mutant of Taq, Tth, Tma, Pfu, Deep, Vent or Tgo DNA polymerase. The invention also provides the application of the recombinant heat-resistant DNA polymerase in DNA sequencing. The continuity and the extending speed of the recombinant heat-resistant DNA polymerase disclosed by the invention are obviously enhanced; the time is shortened during a PCArg reaction sequencing process; by using the sequenase provided by the invention, longer reading length and higher sequencing success rate can be achieved.