3718 results about "Biofilm" patented technology

The invention relates to the technical field of equipment fault prediction, in particular to a coal mine equipment fault prediction system and method based on big data, and the method comprises the following steps: collecting the thickness of a biological membrane on the surface of equipment, synchronously collecting the vibration acceleration of the equipment and the environmental hydrogen sulfide concentration, and constructing a corrosion multi-mode data set; performing electrochemical noise separation on the corrosion multi-modal data set, and constructing a corrosion activity fingerprint spectrum; decoupling a coupling effect of mechanical vibration and microbial corrosion, and generating a corrosion-fatigue damage correlation map; and constructing a dual-channel life prediction model based on the corrosion-fatigue damage correlation map, and setting an early warning mechanism. Microbial corrosion characteristic current is accurately extracted through wavelet packet decomposition and wavelet entropy analysis, a corrosion-vibration heterogeneous graph is established by adopting a graph attention network, corrosion-induced structural vibration enhancement effect analysis is realized, and compared with a traditional prediction method based on a single signal, the method has the advantages that the method is simple and convenient to operate. And the problems of limited local corrosion influence range, unstable vibration response and the like are effectively solved.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention relates to the technical field of nano material application. The invention provides a ruthenium-doped Prussian blue nano-enzyme and a preparation method thereof. The ruthenium-doped Prussian blue nano-enzyme is obtained by reacting metal ruthenium with Prussian blue nano-particles at normal temperature. The invention further provides a nano-enzyme library containing the ruthenium-doped Prussian blue nano-enzyme and a construction method of the nano-enzyme library. The invention also provides the SNP-PB nano-enzyme obtained by loading sodium nitroprusside on the ruthenium-doped Prussian blue nano-enzyme. The ruthenium-doped Prussian blue nano-enzyme and the SNP-PB nano-enzyme both have good antibacterial and anti-inflammatory performance, after NO gas release capacity is given, the problem of resistance of a biological membrane is better solved, then oxidative stress resistance and periodontal bacterial infection resistance are better exerted, and a new treatment method is provided for periodontal diseases.

The present invention relates to the construction of a high-yield indigo genetic engineering strain and a fermentation method. The present invention uses Escherichia coli as a starting strain, knocks out at least one of the yddG gene, trpR gene, tyrA gene or pheA gene in the host cell; integrates the tryptophanase gene tnaA and / or the flavin monooxygenase gene MaFMO on the genome; integrates one or more genes related to biofilm modification on the genome, and obtains the optimal engineering strain by comparing the fermentation yield; then transfers a plasmid into the strain to further strengthen the tryptophanase gene tnaA and the flavin monooxygenase gene MaFMO; finally, the yield is significantly increased by supplementing carbon sources and substrates and adding surfactants. The present invention provides an indigo-producing genetic engineering strain and a fermentation method with good stability and high synthesis efficiency, providing a reference for the subsequent development of more advantageous engineering strains.

The invention relates to lactobacillus crispatus LC30 for preventing and treating female genital tract infection and application of the lactobacillus crispatus LC30. The lactobacillus crispatus LC30 is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.31923. The lactobacillus crispatus LC30 has good hydrogen peroxide production capacity and adhesion capacity, has a strong inhibition effect on main vaginitis pathogenic bacteria, and can be used for preventing and treating female genital tract infection. The composition is effective to nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the pathogenic bacteria and prevent and treat drug resistance; besides, the fermentation product can reduce the content of pathogenic bacteria metabolites, regulate the secretion of cytokines and achieve the effect of relieving inflammation, so that the fermentation product can be used for preparing products for preventing and treating genital tract pathogenic bacteria infection, preventing and treating vaginitis and / or removing pudendum peculiar smell.

The invention relates to the technical field of microorganisms, in particular to a viable bacterium preparation of a lactobacillus paracasei strain as well as a preparation method and application of the viable bacterium preparation. The viable bacterium preparation comprises a lactobacillus paracasei strain and auxiliary materials, and the preservation number of the lactobacillus paracasei strain is CGMCC (China General Microbiological Culture Collection Center) No.27974. The viable bacterium preparation can inhibit the growth of streptococcus mutans, reduce the formation of a dental plaque biofilm caused by the streptococcus mutans, reduce the content of a mature dental plaque biofilm, inhibit the level of water-insoluble exopolysaccharides generated by the streptococcus mutans, reduce the expression quantity of cariogenic genes gtfB, gtfC, gtfD, ftf and LuxS of the streptococcus mutans, improve the cariogenic activity of the streptococcus mutans and improve the cariogenic activity of the streptococcus mutans. The adhesion rate of streptococcus mutans on a hydroxyapatite tooth model is reduced, and a beneficial in-vitro probiotic function is achieved.

The invention relates to the technical field of environmental pollution monitoring, in particular to an on-line environmental pollution monitoring method and system.The method comprises the steps that original acoustic signals of the inner side wall and the outer side wall of a curve are collected in real time in a high-risk monitoring area, propagation path compensation is conducted, and an inner side acoustic characteristic parameter set and an outer side acoustic characteristic parameter set are obtained; analyzing to obtain thickness values and adhesion strength values of biological membranes on the inner side and the outer side of the curve; inner and outer side falling risk factors are obtained through calculation, and a comprehensive falling risk value of the inner and outer side falling risk factors is calculated; when the comprehensive shedding risk value exceeds a preset shedding risk threshold value, generating biological membrane shedding risk early warning information; the monitoring problem of spatial distribution heterogeneity of the biological membrane at the pipe network bend is effectively solved, and accurate early warning of the falling risk of the biological membrane in the bend area is achieved.

The invention discloses a lactobacillus delbrueckii subsp. Lactis TG001 and application thereof in improving oral health, and belongs to the technical field of probiotic application, the preservation number of the lactobacillus delbrueckii subsp. Lactis TG001 is CCTCC NO: M 20241941, the preservation place is China Center for Type Culture Collection in Wuhan, and the preservation date is September 9, 2024. The lactobacillus delbrueckii subsp. Lactis TG001 has a good inhibition effect on common oral pathogens such as streptococcus mutans and porphyromonas gingivalis, particularly can inhibit the growth of cariogenic bacteria streptococcus mutans by generating hydrogen peroxide and remove biofilm formation, and has good tolerance to antibacterial substances in an oral environment. Therefore, the oral cavity diseases such as dental caries, periodontitis and ozostomia can be prevented, the micro-ecological environment of the oral cavity is effectively improved, meanwhile, the peculiar smell of the oral cavity is relieved, and good application prospects are achieved in preparation of products for preventing and / or treating the oral cavity diseases such as dental caries and periodontitis.

The invention relates to a universal nano biological vesicle preparation method. The preparation method comprises the following steps: carrying out crude extraction on cells from bacteria, fungi, plants or animals to obtain a crude extracting solution; the crude extracting solution is placed in a low-temperature and high-pressure homogenizer to be subjected to low-temperature and high-pressure homogenizing treatment, then the nano vesicles are obtained, the low-temperature and high-pressure homogenizer comprises a high-pressure cavity, a homogenizing valve, a working cavity, a conveying system and a temperature control system, a slit is formed in the homogenizing valve, and the crude extracting solution located in the high-pressure cavity enters the working cavity through the slit of the homogenizing valve, the pressure intensity of the high-pressure cavity is 300-3000 bar, and the working temperature of the high-pressure homogenizer is 0-10 DEG C. The activation energy is reduced by controlling high-pressure and low-temperature environments, so that the original cell membrane structure is broken, the cell wall structure is destroyed, and phospholipid bimolecules are driven to aggregate and self-assemble to form the vesicles. For biological membranes derived from bacteria, fungi, plants and animals, the vesicles can be prepared by the preparation method disclosed by the invention, and the method has universality.

The invention provides a plant lactobacillus KSFY202408 strain and application thereof, and belongs to the technical field of microorganisms. The preservation number of the plant lactobacillus KSFY202408 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 31102. The survival rate of the strain in artificial gastric juice with the pH value of 3.0 can reach 55.2%, the survival rate of the strain in artificial gastric juice with the pH value of 2.5 can reach 33.2%, the activity of a helicobacter pylori strain can be remarkably inhibited, formation of a helicobacter pylori thallus biological membrane can be inhibited, and the activity of helicobacter pylori metabolite urease can be reduced. Meanwhile, the bacterial strain can remarkably protect cell membrane integrity and cell viability of gastric mucosa epithelial cells, and has important significance on prevention or treatment of gastritis, gastric ulcer and other digestive tract diseases caused by helicobacter pylori.

The invention discloses a milk-derived broad-spectrum antibacterial peptide and application thereof, and relates to the technical field of biology. The amino acid sequence of the antibacterial peptide is as shown in any one of SEQ ID NO.2-6. Five novel antibacterial peptides are obtained by carrying out symmetrical structure design and amino acid mutation on the basis of a template peptide, and tests find that the five novel antibacterial peptides have broad-spectrum antibacterial activity which is obviously higher than that of the template peptide, and basically have no cytotoxicity. Wherein the geometric mean (GM) of the minimum inhibitory concentration can reach 3.60, and the therapeutic index (TI) can reach 71.11. The antibacterial peptide prepared by the invention still keeps good antibacterial activity in physiological salt concentration and protease environment, has certain serum stability, and has higher application value; in addition, the antibacterial peptide has a good inhibition effect on the formation of a bacterial biofilm, and has a synergistic effect with traditional antibiotics.

The invention discloses a double-sterilization-anti-sticking composite cross-linked coating as well as a preparation method and application thereof, and belongs to the technical field of functional polymer materials. The coating comprises the following raw materials in parts by weight: 5-30 parts of a cross-linkable release type multifunctional branched polymer and 30-95 parts of a cross-linkable functional polymer. According to the double-sterilization-anti-adhesion composite cross-linked coating, the advantages of contact sterilization, antibacterial factor release and adhesion resistance can be combined together, on one hand, the super-hydrophilic component can effectively prevent initial adhesion of bacteria and protein, and even if a small amount of bacteria fall into the surface of the coating, the contact sterilization component can effectively kill a small amount of adhesion bacteria; the formation of bacteria and biological membranes is avoided; in addition, the introduction of releasable antibacterial molecules can effectively enhance the antibacterial effect, and the purpose of deeply killing pathogens is achieved.

The invention discloses rapid denitrification composite carbon source particles as well as a preparation method and application thereof. The rapid denitrification composite carbon source particle is prepared from raw materials including a slow-release skeleton, a carbon source and an optional functional modified material. The invention integrates the advantages of two solid carbon sources (natural carbon source and artificially synthesized carbon source), and develops novel composite carbon source particles with excellent performance and high cost effectiveness by designing and regulating the formula, proportion and structure. The prepared rapid denitrification composite carbon source particles can ensure that microorganisms have stable carbon source supply in the whole denitrification process, and the denitrification efficiency reduction caused by discontinuous or non-uniform carbon source supply is avoided. The rapid denitrification composite carbon source particles prepared by the invention also have the characteristics of easy biofilm formation, easy fluidization, stable carbon release, lasting carbon supply, relatively high mechanical strength, low cost, wide application range, difficulty in breaking, long service cycle and large specific surface area, and can be well applied to a biological sewage treatment system as a microbial carrier.

The invention discloses a method for repairing arsenic-cadmium combined polluted rice field soil, which comprises the following steps of: reducing the redox potential Eh value of the soil to-100 to-300mV by controlling the height of a flooded water layer of the polluted soil and adding a reducing agent, reducing arsenic in the soil into trivalent arsenic, and simultaneously constructing an indigenous microbial community with high arsenic methylation capability; oxidizing by draining and drying, methylating trivalent arsenic by indigenous microbial communities, and gradually volatilizing arsenic through methylation; and applying a carbon-based functional biological membrane mainly comprising a cadmium passivation flora to stably passivate cadmium. According to the method, the arsenic methylation capacity of microorganisms is improved by precisely regulating and controlling the Eh value, in-situ passivation of cadmium is enhanced through the synergistic effect of the carbon-based carrier and the composite flora, synchronous enhancement of arsenic methylation volatilization and cadmium biological membrane passivation is achieved, the difficulty that an arsenic and cadmium pollution treatment technology is'helpful 'is converted into an acting point of technical breakthrough, and the method is suitable for large-scale popularization and application. The arsenic and cadmium co-treatment of the combined polluted soil is realized.

A device and method for enhancing nitrogen and phosphorus removal based on a multistage AO partial denitrification coupled with Anammox process in combination with a sludge hydrolytic acidification mixture belong to the technical field of active sludge method wastewater treatment. A system includes a water tank, a water pump, a biochemical reaction zone, a hydrolytic acidification tank and other devices. A multistage AO step-feed pipeline is used to inject raw water into the reaction zone in a segmented manner, guaranteeing efficient utilization of organic matter in the raw water; biofilm carriers are added into an anaerobic zone and anoxic zones to enrich anammox bacteria, and nitrite nitrogen produced by partial denitrification provides a substrate for the anammox bacteria to realize autotrophic nitrogen removal; a nitrification and phosphorus accumulating bacteria aerobic phosphorus uptake are performed in aerobic zones; and part of excess sludge in a secondary sedimentation tank enters the hydrolytic acidification tank to convert macromolecular organic matter into low molecular weight organic matter, a hydrolytic acidification mixture and the excess sludge in the secondary sedimentation tank synchronously flow back to the anaerobic zone, and as a high-quality carbon source, the low molecular weight organic matter can promote partial denitrification. The system provides a novel method for efficient and energy-saving treatment of municipal wastewater.

Provided are a composite carbon source particle for rapid denitrification, and a preparation method therefor and the use thereof. The composite carbon source particle for rapid denitrification is prepared from raw materials including a sustained-release framework, a carbon source and an optional functionalized modification material. The composite carbon source particle for rapid denitrification can ensure the stable supply of a carbon source to microorganisms during the whole denitrification process, prevents a reduction in denitrification efficiency caused by discontinuous or non-uniform supply of a carbon source, has the characteristics of easily forming a biofilm, good fluidizability, stable carbon release, long-lasting carbon supply, relatively high mechanical strength, low cost, a wide application range, low risk of fragmentation, a long service cycle and a large specific surface area, and can be applied as a microbial carrier to a biological sewage treatment system.

The invention discloses a high-entropy alloy nanoparticle as well as application and a preparation method thereof, and belongs to the technical field of mature biological membrane removal. The high-entropy alloy nanoparticles comprise the following raw materials: metal powder Fe, Ni, Mn, Nb, Cu and Ag; wherein the molar ratio of Fe to Ni to Mn is 1: 1: 1; the molar ratio of Nb to Fe is x, the molar ratio of Cu to Fe is y, the molar ratio of Ag to Fe is z, x is larger than or equal to 1.0 and smaller than or equal to 12.0, y is larger than or equal to 0.5 and smaller than or equal to 1.5, and z is larger than or equal to 0.05 and smaller than or equal to 0.25. According to the high-entropy alloy nano-particles, the biological membrane removal effect is further enhanced through component regulation and control, and the high-entropy alloy nano-particles have excellent photothermal conversion capacity under sunlight irradiation, so that the biological membrane removal performance is more excellent; and an efficient and long-term stable biofilm removal effect can be realized by using a very small amount of high-entropy alloy nanoparticles.

The subject of this invention is to use beneficial reactive support media or biofilms in the form of reactive support bases or stratums that provide structural (including dimensions) or biochemical benefits to the growth or function (including agglutination) of biofilms. The functional aspect includes the provision of reactivity to a polymeric, cellulosic or silicic framework. The functions are supplied to the structural media during manufacture, conditioning, regeneration or during colonization of biofilms. The structural support media may include biodegradable or refractory polymers / plastics, alginates or uronic acids or extracted bacterial EPS. These materials are reacted, retained or removed based on their physical characteristics.

The invention discloses a salt-tolerant growth-promoting bacillus cereus strain and an application thereof. In particular discloses bacillus cereus T146, and the preservation number of the bacillus cereus T146 is CGMCC (China General Microbiological Culture Collection Center) No.30429. The bacillus cereus T146 disclosed by the invention has good biofilm production, siderophore secretion and nitrogen fixation capabilities, and can remarkably increase the root length, the overground dry weight, the plant height, the number of lateral roots and the average density of the lateral roots of wheat under the condition of salt stress. According to the invention, a low-cost, efficient and applicable culture medium aiming at the bacillus cereus T146 is determined, a stable and controllable fermentation system is established, the preparation method of the microbial inoculum with high mature spore number is obtained by optimizing a spray drying process, and the active spore number of the microbial inoculum can reach 1.3 * 10 < 11 > CFU / g. The bacillus cereus T146 has good development and application prospects in the fields of microbial agents, microbial fertilizers, soil improvement and the like.

A microbial electrochemical filter and a method for using same to remove iron, manganese and ammonia nitrogen from underground water, which belong to the technical field of wastewater treatment. The filter and the method solve the problem of existing biological filters having poorer operation performance and stability under oligotrophic conditions. The microbial electrochemical filter comprises a cylinder (1), an electrochemical system, and a filter column (4), wherein the electrochemical system and the filter column (4) are arranged in the cylinder (1); the electrochemical system is arranged above the filter column (4); the filter column (4) consists of a carrier, a surface of which is covered with a biological film; and a water outlet (8) is provided in a lower portion of a side wall of the cylinder (1), and an overflow port is provided in an upper portion of the side wall of the cylinder (1). In the method, by means of an electro-oxidation process, some pollutants are removed before entering a filter column (4), thus reducing the bioremoval pressure in the filter column (4); the abundance and carbon sequestration capability of autotrophic microbes in a biological filter are enhanced by means of an electrochemical system, thereby providing an additional carbon source and an additional electron acceptor for the autotrophic biological filter, and prompting the growth and metabolism of biological communities; and the microbial electrochemical filter provides conditions for the growth of heterotrophic iron-manganese oxidizing flora, and achieves the generation and coverage of more iron-manganese active oxide films on the surfaces of filter materials.

The invention discloses a bacillus velezensis strain and an application thereof in disease prevention and growth promotion, the strain is preserved in the China General Microbiological Culture Collection Center on May 14, 2024, the preservation number is CGMCC No.30619, and the strain is named as bacillus velezensis. The invention further discloses a preparation method of the bacillus velezensis strain, and the application of the bacillus velezensis strain in disease prevention and growth promotion of the bacillus velezensis strain in disease prevention and growth promotion of the bacillus velezensis strain in disease prevention and growth promotion of the bacillus velezensis strain. When the strain is applied to the field of agricultural biological prevention and control, a biological membrane can be formed and stably colonized at the rhizosphere of a plant, a relatively strong and broad-spectrum inhibition effect can be generated on various plant pathogenic fungi, and substances such as IAA and siderophores can be generated to promote plant growth. The bacillus velezensis suspension has a prevention effect of 56.36% on cucumber fusarium wilt potted plants, has a certain growth promoting effect on cucumbers, is a biocontrol strain with excellent performance, and has good development and application prospects.

The invention belongs to the field of water ecological restoration, and particularly relates to a riverway water ecological restoration method based on an ecological floating blanket, ecological floating blanket-fish-microorganism is utilized to construct an ecological restoration system to improve a riverway water body, and the ecological floating blanket comprises plants, a carrier, a nylon net, a planting matrix and an inorganic filler; the selected plants are subjected to pollutant degrading bacterium inoculation and tolerance experiment domestication; inorganic filler is hung on the lower part of the ecological floating blanket through a nylon net; the nylon net is subjected to membrane pre-hanging treatment, so that a layer of yellowish-brown biological membrane is formed on the surface of the nylon net; the planting substrate is a combination of multiple of chitosan-potassium alginate coating melamine polyurethane composite foam, biochar, scrap iron and alum sludge ceramsite; the plant provided by the invention is high in tolerance and can efficiently adsorb and degrade pollutants; the planting matrix provided by the invention promotes each other and efficiently adsorbs pollutants; the constructed ecological floating blanket-fish-microorganism ecological planting and breeding technology can promote a receiving river channel to form a food chain system with a healthy water body.

The present invention discloses a biofilm multi-stage treatment device for industrial wastewater with a telescopic mechanism, including a multi-stage treatment device body, a biological reaction tank and an aeration rod, the bottom of the aeration rod is fixedly connected to an aeration pipe, the outer wall of the aeration pipe is fixedly connected to an air dispersion nozzle, and the treatment device also includes: a receiving block and a disc. In the present invention, the first screw is driven to rotate by rotating the handle, and the two groups of first screw sleeves on the first screw move synchronously toward the center or both sides, the first connecting rod and the second connecting rod are fixedly connected to the first screw sleeve through a fixed rod, and the center of the first connecting rod and the second connecting rod is connected through a rotating shaft, so that the first connecting rod and the second connecting rod are synchronously expanded outward or contracted inward, and the disc at the bottom can synchronously adjust the spacing accordingly, thereby improving the growth environment of microorganisms, improving the purification efficiency of microorganisms, and improving the use effect of the product.

The invention relates to the technical field of wastewater treatment, in particular to a hydrolytic acidification wastewater treatment process for strengthening extracellular electron transfer, which comprises the following steps: S1, constructing a hydrolytic acidification reactor, taking carbon fiber suspension filler as an anode, taking a steel wire mesh frame as a cathode, connecting the carbon fiber suspension filler in series by a stainless steel wire rope, and taking the stainless steel wire rope as a cathode; the carbon fiber suspension filler is connected with the steel wire mesh frame through an electrochemical workstation; s2, inoculating hydrolytic acidification bacteria into a reactor, controlling culture conditions, and domesticating microorganisms in a micro-current environment until a biological membrane is formed on the carbon fiber suspension filler; s3, inputting wastewater to be treated into the reactor, controlling treatment conditions, monitoring an effluent result, and discharging after the effluent result reaches the standard. Compared with traditional interspecific electron transfer, the wastewater treatment method has higher efficiency and speed, the oxygenolysis process of organic matter is accelerated, and the overall efficiency of wastewater treatment is improved.

The invention discloses a lactobacillus casei KC88, and the preservation number of the lactobacillus casei KC88 is CGMCC (China General Microbiological Culture Collection Center) No.32107. Meanwhile, the invention also discloses a screening process and application of the strain. According to the lactobacillus casei KC88 provided by the invention, the expression of a Cdl gene with fusobacterium nucleatum can be down-regulated, and the expression of a Mgl gene of porphyromonas gingivalis can be down-regulated. At the same time, the lactobacillus casei KC88 can inhibit the production of pathogenic bacteria biological membranes and reduce the production of volatile sulfides. And the inactivated surface protein of the casei casei KC88 can inhibit the formation of a pathogenic bacterium biological membrane. Therefore, the lactobacillus casei KC88 viable bacteria and the thermally inactivated bacteria have the effects of relieving breath and improving gastrointestinal functions, and can be applied to the fields of medicines, health care products and the like.

The invention discloses a nano-antibacterial agent for treating biofilm infection through Prussian blue and a preparation method and application thereof.According to the nano-antibacterial agent, hollow Prussian blue (HPB) serves as a carrier to load a substance with anti-inflammatory and anti-oxidation effects, a metal polyphenol network structure is formed through chelating of tannic acid (TA) modified by hyaluronic acid (HA) and high-valence metal ions, and the nano-antibacterial agent is used for treating biofilm infection through Prussian blue. And the MPNs are packaged to obtain the product. All the components of the nano antibacterial agent have a synergistic effect, MPNs formed by chelating TA modified by hyaluronic acid and high-valence metal ions in the antibacterial agent have good photo-thermal performance, and light energy can be converted into heat energy for photo-thermal therapy. According to the invention, the nanoparticles for photo-thermal antisepsis and anti-inflammation are constructed, so that comprehensive treatment of anti-biofilm and anti-inflammatory effect is realized, and healing of infected parts is promoted. And the antibacterial agent has good biocompatibility and high biological safety, and is very suitable for replacing antibiotics to treat biological membrane infection.

The invention discloses lactobacillus plantarum and application thereof, and aims at solving the technical problems of how to inhibit the growth of malassezia furfur, splitting the malassezia furfur, and inhibiting the growth of pseudomonas aeruginosa and staphylococcus aureus and the formation of a biological membrane. In particular discloses lactobacillus plantarum, the lactobacillus plantarum is lactobacillus plantarum DZ041, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.20835 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms). Bacterial suspension or cell-free fermentation liquor prepared from the malassezia furfur can inhibit the growth of malassezia furfur and even can crack malassezia furfur, and the malassezia furfur can be used for industrial production.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The invention discloses a device and a method for realizing low-carbon-nitrogen-ratio sewage autotrophic and heterotrophic coupled deep nitrogen and phosphorus removal based on a continuous flow membrane aeration bio-membrane reactor. A hollow fiber membrane is arranged in the continuous flow reactor, and an oxygen concentration gradient is formed through a bubble-free membrane aeration technology. In the biomembrane area, spatial partitions of functional bacteria such as nitrifying bacteria and anaerobic ammonium oxidation bacteria are established, and the two anoxic areas realize efficient nitrogen removal through short-cut denitrification coupling anaerobic ammonium oxidation (PD / A) and synchronous nitrification and denitrification. In the aspect of phosphorus removal, the system adopts an anaerobic-aerobic operation mode, phosphorus-accumulating bacteria release phosphorus anaerobically and absorb excessive phosphorus aerobically, and finally, phosphorus removal is realized through residual sludge discharge. The system integrates an internal carbon source extraction process, recovers a sludge carbon source and supplements an electron donor required by denitrification, and reduces the operation cost and carbon emission. The continuous flow MABR denitrification system enhances the denitrification efficiency of the PD / A system, is suitable for large-scale operation, and efficiently and stably realizes deep denitrification of a low-carbon-nitrogen-ratio urban sewage treatment plant.

The invention relates to a bacteria-algae composite adsorption module wetland substrate filler as well as a preparation method and application thereof, and belongs to the technical field of biology. The bacteria-algae composite adsorption module wetland substrate filler is prepared by the following steps: preparing an adsorption module, fungicide microspheres and a diatom biological membrane layer, placing the diatom biological membrane layer on the upper layer of the fungicide microspheres to form a diatom fungicide composite layer, and placing the diatom fungicide composite layer on the upper layer of the adsorption module. The bacteria-algae composite adsorption module wetland substrate filler disclosed by the invention can effectively remove pollutants such as nitrogen, phosphorus, lead, cadmium and the like in sewage by independently using the bacteria-algae composite adsorption module wetland substrate filler, effectively reduces the chemical oxygen demand (COD) of the sewage, and simplifies the hierarchical design of an artificial wetland; and the regeneration capacity is high, the replacement period is prolonged, and the operation and maintenance cost is reduced.