1237 results about "Resistant genes" patented technology

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention discloses two polypogon fugax herbicide-resistant genes and application thereof, and belongs to the technical field of herbicide-resistant genes. The invention discloses CDS sequences of PfGSTup and PfGSTl1 genes of polypogon fugax for the first time, the CDS sequence of the PfGSTup gene is as shown in SEQ ID NO. 1, and the CDS sequence of the PfGSTl1 gene is as shown in SEQ ID NO. 4; it is found for the first time that transgenic escherichia coli can obtain mesosulfuron-methyl resistance by expressing PfGSTup and PfGSTl1 genes in escherichia coli, and two new mesosulfuron-methyl resistance GST family candidate genes derived from polypogon fugax are provided; the provided PfGSTup and PfGSTl1 genes can be used for culturing ALS inhibitor herbicide-resistant wheat varieties, and two candidate genes are provided for researching the ALS inhibitor herbicide-resistant mechanism of the polypogon fugax, detecting the resistance of the polypogon fugax and determining the medication period and the dosage.

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention relates to the technical field of solid waste treatment, in particular to a method for removing antibiotics and resistance genes in aerobic composting of chicken manure. Comprising the following steps: adding a composting auxiliary material into chicken manure to enable the carbon-nitrogen ratio to be (25-30): 1, and adjusting the initial moisture content to 55-65% to obtain an initial composting material; adding persulfate and zero-valent iron into the initial compost material, and uniformly mixing; loading the mixed material into a container for composting; during the composting period, stirring is performed regularly, and the aerobic composting time is 30-40 days. The method has the advantages that the persulfate advanced oxidation technology and the aerobic composting technology are combined, the potassium persulfate and the zero-valent iron are added to serve as the aerobic composting additive, the zero-valent iron can continuously activate the potassium persulfate, persulfate free radicals and hydroxyl free radicals are generated, and removal of antibiotics and antibiotic resistance genes is promoted; high-concentration oxytetracycline, sulfamethoxazole and ciprofloxacin in the chicken manure are effectively removed; and the abundance of antibiotic resistance genes with high risk levels is obviously reduced.

The invention provides a method for intensively removing antibiotic resistance genes in a composting process, and belongs to the technical field of solid waste treatment. The method comprises the following steps: performing aerobic composting on compost materials, and when the temperature in a compost body is reduced to 38-42 DEG C, inoculating a microbial preparation; the microbial preparation is a microbial agent with a function of promoting degradation of organic matters. Based on the principle that a microbial preparation is inoculated in a compost cooling period (38-42 DEG C), a microbial community structure is changed, and ARGs host bacterium proliferation is effectively inhibited, the effect of strengthening removal of resistance genes in the compost process is remarkable, operation is easy and convenient, control and implementation are easy, secondary pollution cannot be caused in the whole process, and the method is suitable for industrial production. The method has a wide prospect of popularization and application in a large range.

The invention relates to a primer and probe combination for nested PCR detection of urinary tract klebsiella pneumoniae and drug-resistant genes and a kit thereof, and primer and probe sequences designed in the invention do not cause cross amplification of other urinary tract pathogenic bacteria, and can meet specific requirements. In addition, exogenous antibiotic interferents such as gentamicin, tobramycin, ceftazidime, levofloxacin, piperacillin and nitrofurantoin have no influence on experimental results, and the anti-interference requirement is met. The prepared kit can be used for simultaneously detecting the urinary tract klebsiella pneumoniae and the drug-resistant gene, and the detection is convenient, simple and reliable. Compared with common strain identification, drug resistance gene detection can guide clinical medication more accurately, and invalid treatment is avoided.

The invention discloses application of an LRK20 gene in regulation and control of rice disease resistance, a related biological material and a cultivation method. According to the application of the LRK20 gene in regulation and control of rice disease resistance, the sequence of the LRK20 gene is shown as SEQ ID NO.1, and the disease resistance is sheath blight resistance. The amino acid sequence of the protein coded by the LRK20 gene is as shown in SEQ ID NO. 2. The recombinant vector comprises the complementary sequence of the LRK20 gene in the application. The novel gene LRK20 for regulating and controlling the resistance of the rice sheath blight disease is obtained through identification, the sheath blight resistance gene LRK20 can regulate and control the resistance of the rice sheath blight disease, the resistance of the rice to the sheath blight disease can be remarkably weakened by knocking out the gene, and the resistance of the rice to the sheath blight disease can be remarkably enhanced by overexpressing the gene; therefore, the gene has a potential breeding application value in sheath blight resistance molecular breeding.

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention relates to the technical field of gene engineering, and discloses a rice flood-resistant gene ST4 and application thereof. The nucleotide sequence of the rice flood-resistant gene ST4 is as shown in SEQ ID NO.1, and the coding sequence of the rice flood-resistant gene ST4 is as shown in SEQ ID NO.2. The amino acid sequence of the rice flooding-resistant protein encoded by the rice flooding-resistant gene ST4 is shown as SEQ ID NO.3. By adopting the rice flood-tolerant gene ST4 and the application thereof, the rice flood-tolerant gene ST4 is over-expressed in a rice plant, the germination rate and the seedling rate of rice can be obviously improved, and a foundation is laid for cultivating flood-tolerant rice.

The invention relates to the technical field of gene isolation and plant improvement, in particular to a late blight resistance gene, a biological material and application. The Rpi-card1 nucleic acid molecule provided by the invention comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-4; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 5 or 6; the nucleotide sequence has at least 75% sequence identity with the nucleotide sequence. The invention provides an important gene resource for potato late blight resistance breeding, overcomes the problem of disease resistance loss of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.

The invention relates to the technical field of plant genetic engineering, in particular to a gene of leymus secalinus participating in adversity stress and application of the gene. The gene LsHSFA participating in adversity stress in leymus secalinus is found for the first time, the nucleotide sequence of the gene LsHSFA is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO.2. Through cloning and identification of the adversity stress participating gene of the leymus secalinus, expression analysis of the gene, genetic transformation of the gene and other means, the function of the gene is analyzed and verified, and the result shows that the adversity stress participating gene of the leymus secalinus has important influence on promotion of upper development of the leymus secalinus and improvement of heat resistance, salt resistance, drought resistance and other stress resistance; the stress-resistant gene of the leymus secalinus is utilized to positively regulate and control the abiotic stress function, so that the leymus secalinus can cope with various stress adversities better, the leymus secalinus can adapt to more complex and harsh ecological environments, degenerated and desertified grassland is improved, and potential application prospects are provided for improved variety breeding and excellent population construction of the leymus secalinus in the future.

The invention relates to cloning of a stress-resistant gene LpbHLH144 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH144 of Lilium pumilum DC. Comprising the following steps: (1) cloning a target gene; (2) constructing an expression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH144 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the functions of overexpression of the LpbHLH144 for improving the saline-alkaline and drought stress of the tobacco are identified through transgenic tobacco, the functions of the lilium tenuifolium bHLH gene when the lilium tenuifolium bHLH gene is subjected to adversity stress are disclosed, a foundation is laid for further application of the lilium tenuifolium in lily breeding, and a theoretical basis is provided for the functions and effects of bHLH transcription factors.

The invention provides a kit for rapidly detecting MRSA and drug-resistant gene nucleic acid POCT of the MRSA based on a phage lyase mutant. The kit comprises nucleic acid extraction and purification and triple PCR rapid amplification detection reagents. The staphylococcus aureus bacteriophage lyase mutant nucleic acid release liquid containing the amino acid sequence as shown in SEQ ID NO: 1 is adopted, wall breaking can be rapidly conducted on staphylococcus aureus, and nucleic acid is released. The effect of the nucleic acid release liquid acting for 5 minutes is equivalent to or even better than that of the lysozyme acting for 60 minutes; according to the present invention, with the combination of the MRSA and drug-resistant gene triple PCR rapid amplification detection reagent, the total detection time is only 35 min, the time is saved by 55 min or more than 55 min compared with the lysozyme method, and the MRSA and drug-resistant gene nucleic acid POCT rapid detection can be achieved;

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention provides a pathogen interpretation method and device and a medium, and the method comprises the steps: carrying out the data preprocessing of received initial sequencing data, and obtaining first sequence data; comparing the first sequence data with a reference genome sequence in a preset pathogen database to obtain a comparison result; according to a comparison result, obtaining a read parameter set of the initial sequencing data; performing pathogen interpretation processing on the read parameter set to obtain a target pathogen type corresponding to the initial sequencing data; performing drug-resistant gene detection on the target pathogen type, and judging whether a target drug-resistant gene corresponding to the target pathogen exists or not; if the target drug-resistant gene exists, analyzing the target drug-resistant gene to obtain drug-resistant data; and generating a target report according to the target pathogen type, the target drug-resistant gene and the drug-resistant data. According to the invention, the type of the infected pathogen can be rapidly and accurately interpreted, and the drug resistance condition of the infected pathogen can be accurately analyzed, so as to assist clinicians in formulating a reasonable target report.

The invention provides a functional molecular marker of a wheat leaf rust resistance gene Lr.ace-4A, a detection method and application of the functional molecular marker. The functional molecular marker comprises an upstream primer as shown in SEQ ID NO: 1 and a downstream primer as shown in SEQ ID NO: 2. The method can solve the problem that the Lr.ace-4A gene in wheat is difficult to rapidly detect in the prior art, and is suitable for the field of molecular genetic breeding.

The invention provides a composition, a kit and a detection method for detecting staphylococcus aureus or a drug-resistant gene thereof based on RPA-CRISPR / Cas12a and application of the composition, the kit and the detection method, and belongs to the technical field of gene detection. A CRISPR / Cas12a system is combined with recombinase polymerase amplification (RPA), an RPA-CRISPR / Cas12a detection method for rapidly detecting the staphylococcus aureus in the wastewater and the drug-resistant gene of the staphylococcus aureus is established, a specific primer and crRNA are designed, the high-specificity nucleic acid cutting capability of a gene editing enzyme CRISPR / CAS system and the isothermal rapid amplification advantage of a nucleic acid isothermal amplification technology are fully fused, and the detection method has the advantages of high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity The fluorescent probe has high-specificity sequence recognition capability, realizes efficient detection of target nucleic acid, is strong in detection specificity, high in sensitivity and simple and convenient to operate, does not need complex equipment and professionals, and is suitable for rapid detection in an on-site environment with limited resources.

The invention provides a wheat drought-tolerant gene TaTZF1 and application thereof in plant drought tolerance, and belongs to the technical field of gene engineering. The invention provides a wheat drought-enduring gene TaTZF1. The amino acid sequence of a protein encoded by the wheat drought-enduring gene TaTZF1 is as shown in SEQ ID No. 2. The wheat drought-enduring gene TaTZF1 is subjected to induced expression by drought, ABA and salt stress, the wheat drought-enduring gene TaTZF1 is overexpressed and transformed into wheat in an overexpression mode, and it is found that the drought tolerance of a transgenic plant with overexpressed TaTZF1 is remarkably improved compared with that of a wild type plant; after the wheat drought-tolerant gene TaTZF1 in wheat is knocked out, the drought tolerance is obviously reduced. It is proved that the wheat drought-resistant gene TaTZF1 positively regulates the drought resistance of wheat, gene resources are provided for wheat molecular breeding, and the wheat drought-resistant gene TaTZF1 has great significance in improvement of wheat drought-resistant genetic improvement.

The invention belongs to the technical field of biotechnology, and particularly relates to a wheat thermo-sensitive stripe rust resistant gene TaEDR2-7B, a recombinant vector, application and a method. The nucleotide sequence of the gene TaEDR2-7B is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention provides the gene TaEDR-7B related to the temperature-sensitive stripe rust resistance of the wheat as well as the encoding protein and the application of the gene TaEDR-7B for the first time. The invention discloses that the gene TaEDR2-7B plays an important role in the temperature-sensitive disease resistance of the wheat.

The invention relates to the technical field of cotton alkali-resistant gene mining, and particularly discloses a method for jointly screening key genes responding to upland cotton alkali stress based on transcriptome and metabolome, which comprises the following steps: carrying out alkali stress treatment on upland cotton to obtain tissue samples at different time points; performing transcriptome sequencing and metabolome sequencing on the tissue sample to respectively obtain gene expression data and metabolite data; performing difference analysis on the gene expression data and the metabolite data to obtain DEGs and DAMs; carrying out conjoint analysis on the DEGs and the DAMs, and screening to obtain a key gene and a regulatory pathway which respond to alkali stress; analyzing the key gene and the regulatory pathway through a gene expression verification technology to obtain an expression mode; according to the invention, 15 candidate genes related to the alkali resistance of the upland cotton are screened through WGCNA, a theoretical basis is provided for deeply knowing the molecular mechanism of the alkali resistance of the upland cotton, and a new gene resource is provided for subsequent research.

The invention relates to the technical field of information, and provides a cross-platform global weed gene database unified building method which comprises the following steps: screening out a weed population having gene exchange characteristics with a target crop, identifying gene segments of gene exchange between the crop and weeds by adopting a gene comparison method, and determining a transmission path of resistance genes in interspecific hybridization; according to a gene comparison result, extracting transmission path data of the resistance gene, and analyzing the influence of resistance gene transmission on the genetic structure of the weed population in combination with the genetic structure characteristics of the weed population; calculating the frequency distribution of the resistance genes in different weed populations, judging the diffusion trend of the resistance genes in the weed populations according to the frequency distribution, generating a resistance gene diffusion map, and predicting the variation trend of the future weed population genetic structure based on a weed population genetic structure variation trend chart and the resistance gene diffusion map. And a global weed gene database is established, so that an effective weed management strategy can be formulated.

The invention discloses a gene LRK21 for regulating and controlling the resistance of rice sheath blight disease as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The novel gene LRK21 for regulating and controlling the resistance of the rice sheath blight disease is identified, and the identification result of the resistance of the sheath blight disease of in-vitro leaf sheaths in a climatic chamber proves that the resistance of rice to the sheath blight disease can be weakened by knocking out the gene LRK21, and the resistance of the rice sheath blight disease can be reduced by overexpression of the gene LRK21. The result shows that the LRK21 gene participates in the regulation and control of the resistance of the rice sheath blight disease, and the disorder of the function of the LRK21 can influence the resistance of the rice to the sheath blight disease, so that an important gene resource is provided for the research on the resistance mechanism of the rice sheath blight disease.

The invention belongs to the field of crop genetic breeding, and particularly relates to a rice blast resistant gene OsK8 (Os04g0658700 gene) and application thereof. The invention discloses a rice blast resistance gene OsK8. The nucleotide sequence of the gene is shown as SEQ ID NO: 1. The invention also discloses application of the rice blast-resistant gene OsK8. The OsK8 gene is knocked out from rice, so that the resistance of rice plants to pyricularia oryzae can be effectively enhanced; and the gene Os04g0658700 is overexpressed in the rice, so that the resistance of the rice to the pyricularia grisea is reduced.

The invention discloses three novel corynebacterium glutamicum heat-resistant elements and application thereof, and belongs to the technical field of bioengineering. Three heat-resistant genes are obtained from corynebacterium glutamicum research. The heat-resistant gene and a promoter Ptuf are connected and are combined and transformed in corynebacterium glutamicum. The heat-resistant element is introduced into a plasmid and is expressed in corynebacterium glutamicum. After 24 hours of high-temperature culture at 42 DEG C, the OD600 of the corynebacterium glutamicum containing the heat-resistant element and the plasmid is improved, and the growth condition of the corynebacterium glutamicum at 42 DEG C is obviously superior to that of the starting corynebacterium glutamicum. Therefore, the heat-resistant elements can obviously improve the heat resistance of corynebacterium glutamicum under a high-temperature culture condition. The method has important application value in improvement of the thermal stability of corynebacterium glutamicum and industrial production prospect.

The invention discloses a kit for detecting nucleic acid and drug-resistant genes of pertussis through a high-resolution melting curve and an application method of the kit, and the kit for detecting nucleic acid and drug-resistant gene loci of respiratory tract infection pertussis is developed by combining a PCR (Polymerase Chain Reaction) technology with a high-resolution melting curve analysis technology. The pertussis infection can be diagnosed in the early stage by only using one group of primer probes, and meanwhile, the drug resistance condition of the bordetella pertussis can be accurately judged. And a reliable experimental means is provided for clinical prevention and control of pertussis infection.

The invention belongs to the technical field of gene engineering, and provides a drought-resistant gene OsAP4, a protein coded by the same and application of the drought-resistant gene OsAP4. According to the invention, after the OsAP4 gene is edited in rice, the plant shows drought tolerance under the drought stress condition simulated by PEG6000 treatment; and after the OsAP4 gene is over-expressed in the rice, the drought tolerance of the plant is reduced, which indicates that the drought tolerance of the rice can be regulated and controlled by a method for changing the gene function. An editing carrier containing an OsAP4 editing site is transferred into rice, and the drought tolerance of the rice can be improved by editing the gene; the OsAP4 gene can also be used as a molecular marker to be applied to molecular breeding. The OsAP4 gene has a huge application prospect in molecular breeding and cultivation of drought-resistant rice by utilizing genetic engineering means.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention belongs to the technical field of biological detection, and particularly discloses a targeted sequencing primer group and a kit for detecting common pathogens of children and drug-resistant genes thereof. The primer group comprises 145 primer pairs of common children pathogen primer groups and 67 primer pairs of drug-resistant gene primer groups of the common children pathogen primer groups. According to the kit provided by the invention, nucleic acid in a clinical sample is directly extracted, 212 pairs of specific primers are adopted to realize multi-targeted amplification of a pathogen target area, a high-throughput sequencing platform is adopted to accurately identify pathogens and drug-resistant mutant genes thereof, 72 common children pathogens and drug-resistant genes thereof can be detected at the same time, and the kit has a wide application prospect. More accurate diagnosis information is provided for clinic, a key technical support is provided for early-stage accurate treatment of children infectious diseases, and the technical problems that in the prior art, in children pathogen detection, the detection speed is low, the number of missed detection is large, and the drug-resistant blind area is large are solved.

The invention discloses a primer combination for identifying six genital tract pathogens and detecting drug resistance genes, a kit and application, the primer combination comprises a primer combination for identifying chlamydia trachomatis, mycoplasma genitalium, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis and detecting the drug resistance genes of the six genital tract pathogens, and nucleotide sequences are shown as SEQ ID NO.1-SEQ ID NO.50. Microorganisms and related drug-resistant genes in a detection range are identified by utilizing a multiple PCR targeted three-generation sequencing method, the specificity is good, the sensitivity is high, the detection technology has important clinical significance when being applied to identification of genital tract infection pathogens, and compared with a traditional detection method, the detection flux, the coverage range and the sensitivity are higher, and detection is stable; compared with pathogenic microorganism metagenome (mNGS) detection, the method has the advantages that the detection cost is lower, and the detection sensitivity on drug-resistant genes and drug-resistant gene mutation is higher.