123 results about "Resistant genes" patented technology

The application discloses a method for improving genetic transformation rate of high-altitude meadow grass, comprising the following steps: (1) taking mature seeds of high-altitude meadow grass as explants to induce callus, and obtaining the callus; (2) adding a surfactant Silwet L-77 with a mass fraction of 3‰ of the bacterial liquid to the agrobacterium bacterial liquid carrying a hygromycin resistance gene, and obtaining a culture bacterial liquid; (3) placing the regenerated plant in a screening culture medium containing 40 mg / L of hygromycin resistance for culture, screening the survived plants, and obtaining the genetically transformed plants. The method can improve the transformation efficiency of the high-altitude meadow grass by about 4.54 times through adding the surfactant in the genetic transformation process and cooperating with the screening of the obtained differentiation culture medium.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

GsRPP13L The gene, its recombinant vector, recombinant bacteria and application belong to the technical field of plant disease resistance. In order to solve the technical problems that the utilization of SMV resistance genes and the analysis of disease resistance mechanism are not comprehensive enough in the prior art, a broad-spectrum and durable SMV-resistant soybean variety cannot be quickly and efficiently bred, and the resistance variety is prone to "resistance failure", a soybean gene for improving resistance to soybean mosaic virus is provided GsRPP13L The gene is shown in the nucleotide sequence SEQ ID NO.1, and the gene can improve the resistance of tobacco or soybean to soybean mosaic virus through experiments, and the present application provides a theoretical basis for plant resistance to soybean mosaic virus.

This invention provides a Psa disease resistance gene and its application. Specifically, this invention demonstrates through genetic and molecular experiments that the Psa disease resistance gene is effective against the hardy kiwifruit (Actinidia arguta). Actinidia arguta Wild germplasm Aa11251 containing the G932 protein sequence triggered an immune response upon injection of the HopAO2 effector protein from *Actinidia chinensis*, while materials lacking the G932 protein did not. Transient co-expression experiments on the model plant *Nicotiana benthamiana* showed that co-expression of the resistance protein G932 and the effector protein triggered an HR immune response in the plant. Simultaneously, in *Actinidia chinensis* (Chinese kiwifruit...),... A. chinensis The stable expression of G932 in the transgenic 'Donghong' variety significantly enhances its resistance to pathogens. This indicates that the G932 protein and its encoding gene can be used in agricultural production for the breeding of resistant kiwifruit varieties to bacterial canker. This invention is of great significance for the breeding of disease-resistant kiwifruit varieties and can be used in agricultural production.

This invention discloses a breeding method for improving japonica rice by cultivating polymorphic lines. This invention integrates the GS3 long-grain gene, PigmR disease-resistant gene, fgr aroma gene, ALS herbicide-resistant gene, and COLD1 cold-tolerant gene into japonica rice. The GS3 long-grain trait successfully compensates for the reduction in thousand-grain weight caused by the PigmR gene. Simultaneously, the COLD1 cold-tolerant genotype repairs the genetic defect of low-temperature intolerance in the GS3 donor parent. Furthermore, it demonstrates the heat-resistant function of GS3 in the context of polymorphic lines, simultaneously improving grain shape, blast resistance, aroma, herbicide resistance, heat tolerance, and low-temperature tolerance. This provides new germplasm for the green and efficient production of japonica rice and has significant application value.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

An apple stress-resistant gene MdRPP13-like-1 and its applications, the MdRPP13-like-1 The nucleotide sequence of the gene is shown in SEQ.ID.NO.1. Using transgenic technology driven by the 35S strong promoter, the gene will... MdRPP13-like-1 Gene overexpression vectors were transferred into apples to obtain transgenic apples. This invention is the first to improve plant resistance to continuous cropping using plant genetic engineering technology. A complete coding segment of a disease resistance-related gene was isolated and cloned from apple rootstock, and the function of this gene was verified. Its function was ultimately used to discover the optimal use of [the specific technology / method / approach]. MdRPP13-like-1 MdRPP13-like-1 Transgenic plants that were overexpressed in apples showed significantly improved resistance to continuous cropping.

This invention discloses a novel site-specific recombinase-mediated multisite chromosome editing tool and its applications. The invention provides a complete product suite for integrating or continuously integrating the genome of a host cell, comprising: a circular vector 1 composed of the following elements: loxF1, nox, ori, resistance gene, lox71, P3, P1, P2; and / or a circular vector 2 composed of the following elements: lox66, nox, ori, resistance gene, loxF2, P3, P1, P2; and a circular vector 3 for expressing Nigri and Cre recombinases; and a site-specific knock-in tool for knocking in the lox66-nox fragment at the desired integration site in the host cell genome; P1 and P2 are adapters, used to insert the DNA fragment to be integrated, and P3 is a universal primer. This invention enables rapid and continuous genome integration using simple molecular biology operations.

This invention relates to the field of crop genetics and breeding, and discloses the sweet sorghum disease resistance gene CPK4, its encoded protein, and its applications. The nucleotide sequence of the sweet sorghum disease resistance gene CPK4 is shown in SEQ ID NO.1, or has more than 90% homology with the sequence shown in SEQ ID NO.1 and encodes a protein with a nucleotide sequence that promotes the release of reactive oxygen species (ROS) in plants. By utilizing the CPK4 gene cloned from sweet sorghum, and through the specific interaction between its encoded protein and the NADPH oxidase SbRbohD protein, the ROS signaling pathway induced by pathogens is specifically activated, strengthening the defense response of sweet sorghum against anthracnose. This achieves a targeted enhancement of sweet sorghum's resistance to anthracnose. Furthermore, this gene is specifically induced by the molecular patterns of anthracnose and pathogens, and its disease resistance mechanism differs from existing NLR-mediated immune pathways, enriching the types of sweet sorghum disease resistance gene resources.

The application discloses a pear anthracnose resistance gene MYC2- like and application, and belongs to the field of plant genetic engineering. MYC2‑like The nucleotide sequence of the gene is shown as SEQ ID NO:1, the protein sequence encoded by the gene is shown as SEQ ID NO:2, the gene is significantly up-regulated in response to MeJA and anthracnose fungus induction; the gene is located in the plant cell nucleus and has a conserved bHLH domain; a MYC2‑like MYC2‑like The constructed overexpression vector is used for transforming Solanum lycopersicum callus, so that the resistance of the callus to Colletotrichum gloeosporioides is increased, and the application provides an important gene resource for cultivating pear anthracnose resistance varieties.

This invention discloses an absolute quantification method for pathogens and drug resistance genes in atmospheric bioaerosols and its application. Addressing the bottleneck of achieving absolute quantification of low-concentration samples while maintaining cell integrity and high-concentration nucleic acid extraction, this invention constructs a collection carrier by immobilizing a hydrophilic mixed cellulose ester microporous membrane on a technical agar plate. This is combined with a flow rate ≤30 L / min impactor sampler, ensuring structural integrity while capturing microorganisms, laying the foundation for flow cytometry quantification. This front-end collection system is deeply coupled with a dedicated cascade lysis and concentration process, enabling the acquisition of high-quality DNA suitable for metagenomic library construction and relative abundance analysis even under short-term, low-flow-rate collection. Simultaneously, the total microbial count is absolutely quantified using flow cytometry on the collected samples, and this result is fused with the relative abundance results. Through synergistic optimization of the entire chain, quantitative analysis of species-level pathogens and drug resistance genes in atmospheric bioaerosols is ultimately achieved.

The application belongs to the field of agricultural biotechnology, and particularly relates to a dsRNA of a brown planthopper gene and application of the dsRNA in preventing and treating the brown planthopper and related methods. NlUGT7 The dsRNA of the gene is prepared through in vitro transcription, and RNA interference treatment is performed on the brown planthopper through microinjection. NlUGT7 The results show that after the dsRNA is injected, the expression amount of the gene in the brown planthopper of biotype Y and biotype 1 is significantly reduced. NlUGT7 After the gene is silenced, the survival rate of the brown planthopper of biotype Y feeding on YHY15 rice containing a Bph15 resistance gene or the brown planthopper of biotype 1 feeding on TN1 rice not containing the resistance gene is significantly reduced. NlUGT7 The gene plays an important role in the survival process of the brown planthopper, and can be used as an effective target for preventing and treating the brown planthopper. NlUGT7 The gene and the dsRNA thereof provided by the application can be applied to research and development of biological pesticides for the brown planthopper and green prevention and control, and have good application prospects.

A transcription factor for regulating resistance of kiwifruit to botrytis blight AcWRKY84 The application relates to a gene, and after the expression of the gene is silenced, the resistance of kiwifruit to botrytis blight is significantly reduced. AcWRKY84 The application clones the gene and proves the role of the gene in resisting botrytis blight in kiwifruit, and specifically, after the experimental group is inoculated with the virus, the disease symptoms of the experimental group are more serious, the infected area is obviously increased, and the SOD, POD and CAT enzyme activities in the fruit are significantly reduced compared with those of the control group, so that sufficient gene resources can be provided for a botrytis blight resistance gene library, and the possibility of realizing successful breeding of a high botrytis blight resistance variety is greatly increased.

This invention relates to a primer and probe composition and kit for detecting Mycoplasma pneumoniae and its drug resistance gene mutations. The primer and probe composition includes: a primer and probe set for detecting the wild type of Mycoplasma pneumoniae; a primer and probe set for detecting the A2063G mutation in Mycoplasma pneumoniae; a probe for detecting the A2064G mutation in Mycoplasma pneumoniae; a primer and probe set for detecting a human gene internal standard; and a competitive blocking agent. The primer and probe composition of this invention can detect Mycoplasma pneumoniae and its A2063G and A2064G drug resistance mutation sites with high sensitivity and specificity, solving the technical problems of false positives and insufficient specificity in existing quantitative real-time PCR for detecting single-base mutations in Mycoplasma pneumoniae.

This invention discloses a plasmid for gene overexpression in non-pattern *Bacillus laterosporus*. Its leader region contains a methyltransferase expression cassette derived from the *Bacillus laterosporus* i83 genome, enabling stable existence in specific hosts containing the RM system. Based on this, a PidR2 promoter is added to obtain the pMCPidR2 shuttle plasmid. The PidR2 promoter effectively drives target gene expression, the pWV01 replicon ensures plasmid replication in non-pattern hosts, and the chloramphenicol resistance gene is used for positive colony selection. The plasmid contains a TraJ element, allowing it to be introduced into non-pattern microbial hosts via conjugation transfer. This invention also provides a target gene expression strategy in non-pattern microorganisms, achieving high transformation efficiency and high strain yield through conjugation transfer transformation of donor bacteria, conjugation transfer helper bacteria, and recipient bacteria, resulting in stable expression of the target protein. This invention provides an effective vector for efficient and stable gene overexpression in specific non-pattern microorganisms, helping to solve the problem of difficult genetic manipulation of industrial production strains.

This invention discloses a primer and probe set, kit, and method for detecting carbapenem resistance genes using point-of-care testing (POCT), belonging to the field of gene detection technology. The carbapenem resistance genes include: NDM, KPC, VIM, IMP, OXA, and GES; the primers and probes for the NDM gene include: SEQ ID NO.1, SEQ ID NO.2, and SEQ ID NO.3; the primers and probes for the KPC gene include: SEQ ID NO.4, SEQ ID NO.5, and SEQ ID NO.6; the primers and probes for the VIM gene include: SEQ ID NO.7, SEQ ID NO.8, and SEQ ID NO.9; the primers and probes for the IMP gene include: SEQ ID NO.10, SEQ ID NO.11, and SEQ ID NO.12; the primers and probes for the OXA gene include: SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, and SEQ ID NO.21; and the primers and probes for the GES gene include: SEQ ID NO.22, SEQ ID NO.23, and SEQ ID NO.24. The primer and probe set, kit, and method of this invention can be used to detect carbapenem resistance genes NDM (75 subtypes), KPC (232 subtypes), VIM (90 subtypes), IMP (101 subtypes), GES (64 subtypes), and OXA-48, 23, 51, and 213 groups in a single detection tube. They have the advantages of short detection time, simple operation, wide coverage, good specificity, and prevention of contamination.

This invention provides a molecular marker for detecting the PVR4 gene, a resistance gene against Potato Virus Y, and its application, belonging to the field of genetic engineering technology. The molecular marker is the TC base at 4714-4715 bp of the CM334 pepper variety (NCBI accession number KT359375.1). This invention also provides primers and a kit based on the molecular marker. PCR amplification and electrophoresis detection enable rapid and accurate screening of pepper varieties carrying the PVR4 gene against Potato Virus Y. The molecular marker described in this invention plays an important role in detecting the PVR4 gene against Potato Virus Y in peppers or in breeding disease-resistant pepper varieties.

This invention discloses a PEG-mediated genetic transformation method for *A. anthracnose* protoplasts. Through the preparation, transformation, and transformation of *A. anthracnose* protoplasts, and the screening of transformants, the hygromycin B resistance gene fragment was successfully transferred into the *A. anthracnose* genome, achieving successful genetic transformation of *A. anthracnose* protoplasts. More importantly, this invention establishes a new enzymatic digestion system and improves the culture mechanism in protoplast preparation. During the culture and screening of transformants, a more suitable resistance concentration is used, balancing the stringency of screening with the survival of transformants. This improves transformation efficiency, shortens the overall transformation process, and establishes a more efficient and convenient genetic transformation system for *A. anthracnose* protoplasts.

This invention provides a wheat aphid-resistant gene TaAHL19, its application, and a method for obtaining aphid-resistant wheat varieties. The nucleotide sequence of the wheat aphid-resistant gene TaAHL19 is shown in SEQ ID NO.1. The amino acid sequence encoded by the wheat aphid-resistant gene TaAHL19 is shown in SEQ ID NO.2. In this invention, overexpression of the TaAHL19 gene in wheat plants promotes the accumulation of rutin, an insect-resistant flavonoid, in wheat plants, thereby increasing the resistance of wheat plants to aphids without affecting wheat yield.

The application discloses a kit for detecting 87 respiratory tract pathogens and drug-resistant genes by using a single tube and application thereof. The kit comprises specific primers and probes for the 87 respiratory tract pathogens and drug-resistant genes and is based on a capillary electrophoresis platform. The kit can detect nearly 100 targets and has the advantages of high throughput, high sensitivity, high specificity, simple operation, short TAT time, simple result interpretation and the like, and is suitable for rapid screening and clinical diagnosis of respiratory tract pathogens and drug-resistant genes.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

This invention discloses the application of an electrode material for reducing intracellular resistance genes in drug-resistant bacteria. A platinum-doped dendritic TiO2 nanowire structure based on a three-dimensional material is formed by growing a micron-sized TiO2 backbone array and platinum-doped nano-sized TiO2 branches on the surface of a porous electrode. The prepared dendritic nanowire three-dimensional electrode material has a larger specific surface area, providing more reactive sites and strong electric field sites. Platinum doping enhances the conductivity and electrocatalytic performance of the nanowires. Furthermore, by combining it with chloride salts / oxidants, the electroporation and electrocatalysis of the nanowire electrode surface are coupled. The strong electric field confined at the nanowire tip induces perforation of the drug-resistant bacterial cell structure, strengthening the contact and reaction between the oxidant and the resistance gene. The high electrocatalytic activity confined at the nanowire tip induces the generation of strong oxidizing free radicals, achieving the goal of destroying the drug-resistant bacterial cell structure and efficiently reducing intracellular resistance genes.

This invention discloses a method for modifying a high-yield L-arginine-producing strain and its application in production, belonging to the field of bioengineering technology. This invention integrates E. coli-derived argininosuccinate synthase at the genomic level of E. coli, constructing an argininosuccinate synthase mutant. argG S130N , argG Y132F , argG K133R , argG Y132F / K133R This invention significantly enhanced the conversion of aspartic acid and citrulline to L-arginine, constructing a highly efficient L-arginine-producing strain that is plasmid-free, contains no resistance genes, and requires no induction. The recombinant strain constructed in this invention accumulated 108.5 g / L of L-arginine in a 5L fermenter after 48 hours of fermentation.

The present application relates to the field of environmental microorganism and gene detection technology, and particularly relates to a method for evaluating the chronological evolution of antibiotic resistance genes in lake sediments. 137 Cs, 210 Pb dating method constructs high-precision sedimentary chronology sequence, high-throughput fluorescent quantitative PCR technology is used to detect the abundance of antibiotic resistance genes in the layered samples, and then based on the chronology sequence and gene abundance data, the historical evolution rule of the abundance of antibiotic resistance genes in the time dimension is analyzed. The present application first combines the sediment chronology and high-throughput gene detection technology, realizes the historical reconstruction of the antibiotic resistance genes in the lake, and provides reliable data support for analyzing the evolution driving mechanism and environmental health risk assessment.

The present invention provides a method for producing a target protein by culturing cells, the method being capable of stably maintaining a target plasmid without using an antibiotic. The present invention relates to a method for producing a target substance, the method including a step for culturing cells that are transformed with a target plasmid (A), wherein: each of the transformed cells includes the target plasmid (A) and a temperature-sensitive plasmid (B) and are obtained by a method that includes a step for culturing cells in each of which the function of an essential gene on the chromosome has been reduced at a temperature at which the temperature-sensitive plasmid (B) is eliminated; the target plasmid (A) includes the essential gene and a target substance gene and does not include an antibiotic-resistant gene; and the temperature-sensitive plasmid (B) includes the essential gene and the antibiotic-resistant gene.

This invention provides a molecular marker primer tightly linked to the major brown planthopper resistance gene Bph57 in rice, along with its marking method and application. This invention utilizes genetic linkage analysis of individual F2 plants obtained from crossing the insect-resistant rice variety “Daomushe Late Bengu” (C9) (♂) with 9311 (♀), combined with the brown planthopper resistance levels of F3 families. This analysis identifies the major resistance gene Bph57 carried by the insect-resistant variety C9. The Bph57 region is narrowed down to an approximately 90kb fragment defined by flanking markers 2M26.442 and 2M26.536, and tightly linked to molecular marker 2M26.762. The molecular marker 2M26.762 of this invention effectively detects whether the insect-resistant variety C9 and its derived varieties (lines) contain this major resistance gene locus, significantly improving the selection efficiency for brown planthopper resistance and obtaining brown planthopper-resistant rice varieties (lines) containing Bph57.

This application provides a method for degrading antibiotic resistance genes in penicillin fermentation residue, comprising: Step S1, providing penicillin fermentation residue to be treated. The penicillin fermentation residue contains at least one target antibiotic resistance gene. Step S2, subjecting the penicillin fermentation residue to gamma ray irradiation treatment. This application addresses the problems of low removal rate of antibiotic resistance genes (ARGs), easy rebound, high treatment cost, and high risk of secondary pollution in existing penicillin fermentation residue treatment technologies by subjecting the penicillin fermentation residue to gamma ray irradiation treatment.