9results about How to "Lower titer" patented technology

The present invention relates to a composition for preventing and treating viral infection of the family of African swine fever viruses, in particular, a composition for treating, inhibiting and / or preventing viral infection of the family of African swine fever viruses (Asfaraviridae), preferably African swine fever virus, characterized in that the composition comprises one or more medium-chain fatty acids (MCFAs) and / or derivatives thereof.

This invention discloses a high-density phage culture method for *R. anatidae*, belonging to the technical field of high-density phage culture of *R. anatidae*. First, a standard strain of prevalent *R. anatidae* serotype is selected as the host bacterium. After activation, it is inoculated into an optimized culture medium supplemented with divalent cations. When the host bacterium is in the logarithmic growth phase, it is pre-amplified by inoculating the original phage lysate at a low multiplicity of infection, and high-lytic-activity phage strains are screened. Then, the screened phages are inoculated into an expansion culture system. During the culture process, nutrients are dynamically supplemented to maintain the activity of the host bacterium. After complete lysis of the host bacterium, a specific nuclease is added to the culture system to digest the free nucleic acid. The phage prepared by this invention has a high titer and can be directly used in formulation production without additional concentration. The culture system has low free nucleic acid residue, the phage loss rate during purification is low, and the obtained phage lysis spectrum covers a wide range.

The invention discloses miR-184 and application of the miR-184 in regulating and controlling the reproduction of female diaphorina citri. The nucleotide sequence of the micromolecule miR-184 provided by the invention is shown as SEQ ID NO.4, and the micromolecule miR-184 is highly expressed in the ovary of the female diaphorina citri. By feeding the miR-184 agonist, the egg laying amount of the diaphorina citri is obviously reduced, the development of the ovary is inhibited, the titer of the Candidatus Liberibacter asiaticum in the ovary is obviously reduced, and the miR-184 agonist has a good application prospect in the aspects of regulating and controlling the reproduction of female diaphorina citri, preventing and treating the female diaphorina citri, preventing the Candidatus Liberibacter asiaticum and the like.

This invention discloses the application of bufotoxin lactones in the preparation of anti-IHNV products, belonging to the field of biomedical technology. This invention is the first to discover and confirm that four bufotalin-based drugs (bufotalin, bufotalin ester, bufotalin, and bufotalin) have significant anti-IHNV effects: their IC50 values ​​against IHNV in vitro range from 0.0879 to 0.1809 μM, with selectivity indices all greater than 110. After 48 hours of treatment, they can reduce viral RNA expression by 110 to 173 times and viral titer by 4.36 to 5.15 lg; they also show broad-spectrum inhibitory effects against different genotypes of IHNV, such as LN-15, QH-17, and Blk94; in a rainbow trout in vivo model, after administration of a 0.5 mg / kg dose, the cumulative mortality rate decreased from 95% in the control group to 13.3% to 21.7%, with the highest relative protection rate reaching 85.96% (bufotalin), indicating that these compounds have highly efficient, broad-spectrum, and low-toxicity anti-IHNV activity and have excellent application prospects.

The present application relates to the fields of biotechnology and agricultural fishery, and discloses application of curcumin in preparation of an inhibitor of CyHV-2, and finds that curcumin can effectively inhibit the infection and replication of CyHV-2 virus in GiCF cells.The present application first applies curcumin to the prevention and treatment of CyHV-2 infection, thereby providing a new treatment scheme for CyHV-2 virus.Curcumin can effectively reduce the copy number of CyHV-2 virus, and curcumin can be used to prepare a drug for preventing and treating CyHV-2 infection, and a drug for treating viral hematopoietic organ necrosis in fish.

The present disclosure provides, inter alia, molecules comprising a binding domain that specifically binds to a target antibody and at least one modified Fc domain. The present disclosure also provides methods and compositions that allow for selective depletion and / or neutralization of pathogenic antibodies.

The present application relates to the field of biological medicine, and particularly relates to a preparation for resisting African swine fever virus and application thereof.The preparation is obtained by carbonizing kaempferol in an alkaline environment;the particle size of the kaempferol carbon dots is 1nm-20nm;and the solubility of the kaempferol carbon dots in water is 0.1mg / mL-0.5mg / mL.The kaempferol carbon dots obtained by a solvothermal method have significantly improved water solubility and biocompatibility, reduced cytotoxicity, and are more suitable for use in a drug delivery system.The kaempferol carbon dots have a significant inhibitory effect on African swine fever virus, can reduce virus titer and reduce virus protein expression.The kaempferol carbon dots can inhibit the TLR4 signaling pathway, reduce the production of inflammatory cytokines, and remove active oxygen through SOD-like activity, thereby playing an antiviral role.

This invention relates to a method and application of the inhibitor EHop-016 for inhibiting porcine epidemic diarrhea virus (PEDV) infection in vitro. EHop-016 can be used to prepare antiviral drugs for inhibiting PEDV infection. In this invention, during the in vitro infection of African green monkey kidney cells (Vero) with PEDV, the addition of 4 μmol / L EHop-016 significantly reduced the viral load, protein expression level, RNA content, and viral titer of PEDV in the EHop-016-treated group compared to the control group without EHop-016, indicating that this inhibitor can be used to inhibit porcine epidemic diarrhea virus infection in vitro.

The invention relates to the technical field of microorganisms, and provides a vibrio alginolyticus phage and application thereof in order to screen and accumulate a phage with a good lysis effect, the latin classification name of the vibrio alginolyticus phage is Vibrio alginolyticus phage, the vibrio alginolyticus phage is named as GYN01, the vibrio alginolyticus phage is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.46683, and the vibrio alginolyticus phage is named as GYN01. The invention relates to an icosahedron-shaped tail sheath, which has a typical tadpole-shaped composite symmetrical structure, comprises an icosahedron head part and a retractable tail sheath, a tail plate visible at the tail end of the tail part, a non-retractable tail part and a hollow tail tube visible in the center of the tail sheath, has good temperature stability and a wider acid-base tolerance range, and is sensitive to ultraviolet rays; the compound has good cracking capacity and killing effect on pathogenic vibrio alginolyticus, is good in safety and environment-friendly, and can be used for preventing and treating vibrio alginolyticus diseases in the aquaculture process.