The invention discloses a determination method for acquiring a
denitrification rate based on river dissolved
oxygen high-frequency monitoring. The determination method comprises the following steps: S1, constructing a dynamic
response model of the river
denitrification rate to heterotrophic
respiration; s11, carrying out in-situ high-frequency DO observation on different types of rivers, collecting a
water sample, and measuring the concentrations of dissolved N2 and O2 in the collected
water sample by adopting an MIMS technology; s12, on the basis of the O2 concentration data, the river heterotrophic
oxygen respiration rate is calculated through an O2
mass balance model; s13, based on the N2 concentration data, calculating the river
denitrification rate through an N2
mass balance model; s14, fitting the obtained series of heterotrophic
respiration rates with the denitrification rate to obtain a dynamic
response model between the denitrification rate and heterotrophic respiration; and S2, based on field real-time high-frequency monitoring dissolved
oxygen observation data, simulating the river denitrification rate through the constructed dynamic
response model. According to the invention, the problems that the traditional denitrification
rate measurement cost is high and real-time
continuous monitoring cannot be realized are solved, and in-situ, real-time and high-frequency monitoring of the denitrification rate is realized.