The invention discloses a soybean nodulation number related major
single nucleotide polymorphism (SNP) site and application thereof, the SNP site is positioned on a soybean
chromosome 2, the physical distance of the SNP site in a soybean
genome version number
Glycine max Wm82.a2.v1 is Chr0243129492, the SNP site corresponds to a 20th basic group of a sequence shown as SEQ ID NO.1, when the site is T / T homozygosis, the corresponding
genotype is A, when the site is T / T homozygosis, the corresponding
genotype is B, and when the site is T / T homozygosis, the corresponding
genotype is C; when the site is C / C homozygous, the corresponding genotype is B; the number of root nodules is as follows: the genotype A homozygous soybean is greater than or candidate to be greater than that of the genotype B homozygous soybean. By detecting the SNP, a tested soybean
population can be accurately divided into a low
root nodule number (C / C) and a high
root nodule number (T / T). Therefore, the
molecular marker can be used as a basis for preliminarily identifying the number of soybean nodules, has high efficiency and applicability such as simplicity in operation, low cost and short consumed time, and has important values for digging
germplasm of high-nodulation soybeans, solving the problem of
nitrogen fertilizer abuse and promoting
sustainable development of
agriculture.