Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

90 results about "MUSCLE" patented technology

MUltiple Sequence Comparison by Log-Expectation (MUSCLE) is computer software for multiple sequence alignment of protein and nucleotide sequences. It is licensed as public domain. The method was published by Robert C. Edgar in two papers in 2004. The first paper, published in Nucleic Acids Research, introduced the sequence alignment algorithm. The second paper, published in BMC Bioinformatics, presented more technical details.

Lentiviral plasmid expression vector as well as construction method and application of lentiviral plasmid expression vector

InactiveCN104017827AImprove the level of clinical diagnosis and treatmentImprove the level of diagnosis and treatmentBiological testingFermentationMuscle specific receptor tyrosine kinase AntibodyStaining
The invention discloses a lentiviral plasmid expression vector as well as a construction method and an application of the lentiviral plasmid expression vector. The lentiviral plasmid expression vector is pCDH-MCS-MuSK-GFP and the nucleotide sequence of the lentiviral plasmid expression vector is shown in SEQ ID NO. 2 in a sequence table. The invention further discloses an application of the lentiviral plasmid expression vector in construction of an anti-muscle-specific receptor tyrosine kinase antibody detection. According to the invention, a stable transfected cell line of MuSK is established by constructing a viral vector, a MuSK antibody is qualitatively and quantitatively detected clinically by an immunofluorescence staining method with relatively high sensitivity and specificity in combination with a flow cytometry, the detection results are more stable and manpower and time costs are greatly saved. The diagnosis and treatment levels of myasthenia gravis are greatly improved by the establishment of the project, the labor capacity and life quality of patients are improved and the lentiviral plasmid expression vector has significant social and economic benefits.
Owner:GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV

Fluorescence quantitative PCR detection primer and method for type and composition of yak muscle fibers

The invention discloses a fluorescence quantitative PCR detection method for type and composition of yak muscle fibers. According to the fluorescence quantitative PCR detection method, yak muscle tissues are taken for total RNA extraction, the RNA purity and concentration are detected, the total RNA is subjected to inverse transcription to generate cDNA, then quality detection is carried out by taking GAPDH genes of yak as internal genes, and then a special detection primer is adopted for carrying out fluorescence quantitative PCR reaction, meanwhile, the GAPDH genes of yak are taken as reference genes for statistic analysis. The detection method and the detection primer provided by the invention are more accurate and more reliable than histochemistry evaluation; with the molecule quantitative classification method, accurate quantitative and analysis can be carried out on the type composition of the various muscle fibers in muscles at different parts of the yak, so that a technological base is laid for further study on the relevance between the muscle fibers and the meat quality trait of the yak, and meanwhile, compared with other methods, the fluorescence quantitative PCR detection method has the advantages of low workload and low cost, and high efficiency.
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS

Application of adiponcetin gene expression level in beef cattle muscle marbling grade identification

The invention relates to an application of an adiponcetin gene expression level in beef cattle muscle marbling grade identification, which is characterized in that a cloned adiponcetin gene cDNA complete sequence is used to establish a real-time fluorescence quantitative detection method of adiponcetin mRNA; and the application of the adiponcetin gene expression level in beef cattle muscle marbling grade identification is determined. The method which adopts fluorescence quantitative RT-PCT to detect ADPNmRNA has very good sensitivity and repeatability. In the detection results, a copy number is used to express the content of an initial template, which is more accurate and reliable than a comparison value in semi-quantitative RT-PCR; the ADPNmRNA level is negatively correlated with the muscle marbling of fattening cattle (Yanbian cattle r=-0.87; P<0.001, n=9) and (red steppe cattle r=-0.79; P<0.001, n=9). The accuracy rate is up to 95%.
Owner:JILIN AGRI SCI & TECH COLLEGE

Goat S6K1 gene cDNA encoding zone nucleotide sequence

The invention relates to a cDNA nucleotide sequence which encodes S6K1 protein and is separated from a goat muscle cell and the corresponding amino acid sequence. The invention finds a conservation region by comparing the cDNA nucleotide sequences of the known human, rat, rabbit and cattle S6K1 genes and then designs a pair of primers for a cDNA coding area fragment of an RT-PCR amplified goat S6K1 gene according to the cDNA sequence of a cattle S6K1 gene (GenBank landing number is AY396564), the primers are used for amplifying the specific fragment by the PT-PCR method, the nucleotide sequence of 1563bp of the cDNA coding area of the goat S6K1 gene and the corresponding amino acid sequence are obtained after sequencing. The obtained 1563bp nucleotide sequence can be further used for full-length cDNA cloning of the goat S6K1 gene and detecting the tissue expression specificity of the S6K1 gene by the RT-PCR or the hybridization method, which can also be used for preparing an antibody for detecting the S6K1 after recombinant expression.
Owner:INNER MONGOLIA UNIVERSITY

Method for quickly detecting RFLP (restriction fragment length polymorphism) of SNP (single nucleotide polymorphism) of common ox SIRT1 (silent information regulator 1) gene

The invention discloses a method for quickly detecting RFLP (restriction fragment length polymorphism) of SNP (single nucleotide polymorphism) of a common ox SIRT1 (silent information regulator 1) gene promotor region. The method comprises the following steps of: taking whole genome DNA (deoxyribonucleic acid) of common ox blood as a template and a primer pair P (F and R) as a primer; performing PCR (polymerase chain reaction) amplification on the common ox SIRT1 gene promotor region; digesting a PCR amplification product by using restriction endonuclease SmaI; performing agarose gel electrophoresis on segments after enzyme digestion; and identifying the SNP of the common ox SIRT1 promotor region according to a result of the agarose gel electrophoresis. Because important functions including muscle differentiation, lipogenesis and the like related to the SIRT1 are closely related to common ox meat quality characteristics, and the SNP can significantly affect the promotor activity of the SIRT1 gene and is related to various important economic characteristics of the common ox, the detection method provided by the invention lays a foundation for the establishment of the relationship between the SNP and growth characteristics of the SIRT1 gene, and can be used for marker assisted selection of the growth characteristics of the Chinese common ox so as to facilitate rapid establishment of common ox populations with excellent genetic resources.
Owner:NORTHWEST A & F UNIV

Sinkiang Alaska grayling heritable variation detection method utilizing microsatellite marker Thymalag

The invention discloses a Sinkiang Alaska grayling heritable variation detection method utilizing a microsatellite marker Thymalag. The method comprises the steps that firstly, a genome DNA in Sinkiang Alaska grayling muscle is extracted and diluted for use; then, a microsatellite core sequence contained in a Sinkiang Alaska grayling DNA sequence is utilized, and specific primers are designed at the two ends of the sequence; then, the primers are used for performing PCR amplification on genome DNA of different geographic stocks or individuals inside Sinkiang Alaska grayling, and a PCR product is detected; strips of the product are analyzed, the gene type of each individual is determined, and a genetic polymorphic map of Sinkiang Alaska grayling is obtained. The method has the advantages that the genetic variation map of the genetic marker Thymalag of Sinkiang Alaska grayling can be obtained rapidly, and the method is simple and convenient to implement.
Owner:广东华轻质量检测服务中心有限公司

South China Sea conotoxin coding sequence as well as preparation method and application of South China Sea conotoxin

The invention discloses a South China Sea conotoxin coding sequence as well as a preparation method and application of the South China Sea conotoxin. A new O-super family toxin gene Cq1.0 is discovered from the venom canal of South China Sea conotoxin through a method for constructing a cDNA library and is obtained by utilizing a high-throughput sequencing method. A mouse hot plate experiment and a zebrafish muscle injection experiment discover that the conotoxin Cq1.0 has the effects of central analgesia and nerve anesthesia, can be taken as a probe for the classification and the identification of ion channel types and subtypes or for the research and the development of tool drugs and the preparation of analgesic drugs.
Owner:RES INST OF SUN YAT SEN UNIV & SHENZHEN

Expression plasmid of macrobrachium rosenbergii exogenous gene and efficient transcription expression method thereof

The invention discloses an expression plasmid of a macrobrachium rosenbergii exogenous gene and an efficient transcription expression method of the expression plasmid. The expression plasmid comprises an hr5 enhancer, a transcription promoter IE1, an exogenous target gene and a terminator which are arranged in sequence, the nucleotide sequence of the hr5 enhancer is shown as SEQ ID No: 1, and the nucleotide sequence of the transcription promoter IE1 is shown as SEQ ID No: 2. The method comprises the following steps: inserting a microsyringe from the boundary line of the belly, the head, the chest and the trunk of the macrobrachium and injecting the expression plasmids according to the quantity of 10 micrograms of plasmids per gram of the body weight of the macrobrachium, and controlling the injection quantity of each macrobrachium to be 5-20 microliters; after injecting the exogenous expression plasmid for 4-12 hours, collecting the torso muscle tissue of the macrobrachium rosenbergii, and verifying the expression quantity of the exogenous target gene. According to the method, the intramuscular transcriptional expression level of the macrobrachium rosenbergii exogenous gene is higher and is about 3 times higher than the transcriptional level of the current common promoter IE1; and the method for expressing the exogenous gene in vivo is fast and efficient.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products