61 results about "MUSCLE" patented technology

The invention belongs to the technical field of gene detection, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker, a primer pair, a kit, a detection method and application of a g.64930 site of a PPARG (Peroxisome Peroxisome Activated Receptor Gene) gene of Anhui local variety cattle. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO.1 and SEQ ID NO.2, when the basic group at the 118th site of the 5'end of the molecular marker is G, the meat color brightness of beef is higher than that when the basic group at the site is T, and the muscle pH is lower than that when the basic group at the site is T. The molecular marker can be used as a candidate molecular marker for the quality character of the Anhui local variety cattle, is applied to population breeding of the Anhui local variety cattle, and is combined with production requirements for molecular-assisted breeding, so that a theoretical basis is conveniently provided for variety resource development and breeding of the Anhui local variety cattle.

The invention relates to the technical field of nuclide probes, and discloses a targeted PARP nuclide probe and a preparation method and application thereof, and the targeted PARP nuclide probe comprises a radionuclide labeled compound as shown in formula I; the targeted PARP nuclide probe provided by the invention can be used as a diagnosis and treatment reagent to be applied to tumors with high PARP protein expression in human or animal bodies, and is especially suitable for tumor imaging and radionuclide treatment; animal experiments show that the nuclide probe has a high tumor / muscle uptake ratio and shows good application potential. Formula I.

The invention described here contains a nucleic acid expression cassette comprising a transcriptional regulatory region operably linked to a nucleic acid sequence encoding a FKRP, an RNA transcript comprising a modified 5′ and / or 3′ untranslated region (UTR) that will be utilized to treat a variety of FKRP-mediated diseases.

The present application relates to the technical field of livestock feed, and particularly relates to a feed for improving muscle growth of broilers and application of a molecular marker (His-Thr-Gln-Glu) of the feed in cells, and provides a molecular marker for regulating animal skeletal muscle, and an amino acid sequence of the molecular marker is His-Thr-Gln-Glu, and the molecular marker can be applied to preparation of a product for regulating animal skeletal muscle as a target.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

Compositions and methods for modifying a muscle or muscle precursor cell genome are disclosed. Also provided are methods of treatment using the compositions.

The invention provides a method for constructing a mouse TGIF1 gene knockout cell line based on a CRISPR / Cas9 technology, and belongs to the technical field of gene editing. According to the invention, the CRISPR / Cas9 technology is adopted, the TGIF1 gene in the C2C12 cell is successfully knocked out, the C2C12 cell line with the knocked-out TGIF1 gene is established, the cell line is stable in heredity, and the cell morphology and proliferation are not obviously different from those of a control group cell. The method can be used for researching the function of the TGIF1 gene in mouse muscle development and revealing the action mechanism of the TGIF1 gene in muscle development regulation.

Provided herein are compositions and methods for treating or preventing dilated cardiomyopathy (DCM) in a subject in need thereof, for example, through correcting one or more point mutations in the RBM20 locus using CRISPR-associated base editing. TIFF2025538153000011.tif56128

The invention discloses application of a traceless gene editing cell line in research on influence of IGF-1 on cell proliferation and apoptosis, an interference molecule for inhibiting TM4SF4 expression and application, and relates to the technical field of biomedicine. Comprising the following steps: step 1, replacing an MSTN sequence with an IGF-1 sequence through a CRISPR / Cas9 editing technology; constructing an expression vector capable of expressing IGF-1; step 2, introducing the expression vector in the step 1 into a cell, carrying out amplification culture, extracting a genome of a cell line by using a genome extraction kit, carrying out PCR amplification by using a primer pair, recovering an amplification product, and carrying out sequencing identification; the monoclonal cell line which is identified to be correct is the IGF-1 gene editing cell line; and 3, researching the influence of IGF-1 on cell proliferation and apoptosis by adopting an IGF-1 gene editing cell line which is correctly identified. Regulation of IGF-1 on myoblast proliferation is researched on the basis of a traceless gene editing cell line, and a key regulation gene of a downstream signal channel after MSTN is replaced by IGF-1 is screened in combination with transcriptome sequencing, so that an IGF-1 mediated muscle growth molecular mechanism is clarified.

The application discloses a biological age evaluation method, comprising the following steps: obtaining a key protein index of a person to be evaluated, inputting an improved biological age evaluation model I, and obtaining the biological age of the person to be evaluated; the improved biological age evaluation model I is obtained based on an elastic network regression algorithm by taking proteomic data and body composition age of an individual as training data. The application also discloses a construction method of the model, i.e., a two-step method. The application allows a user to calculate the biological age according to the proteomic data, realizes personalized monitoring of muscle aging state, and has great guiding significance in identifying individuals with early muscle function decline and formulating personalized and accurate anti-aging schemes.

The invention provides a myopathy progress prediction method and device based on a spatio-temporal topology network, and the method comprises the steps: carrying out the image segmentation and quantification of muscle biopsy sections of a patient at a plurality of time points, constructing a static pathological topology network with a single muscle fiber as a node and a spatial adjacency relation as an edge, and enabling the node to comprise the multi-dimensional features of subtypes, forms, textures, cell nucleuses and the like; and forming time-space diagram data according to a time sequence. The data are input into a pre-trained space-time diagram neural network, a space coding layer aggregates neighborhood information through a diagram convolutional network to capture space interaction, a time coding layer learns a time sequence evolution rule through a recurrent neural network, and a decoding layer predicts a network state of a next time point. Pathological network sequences of multiple time points in the future are generated through iterative prediction, then key indexes are extracted to draw a quantitative prediction curve, a visual pathology heat map is generated, crossing from static pathology to dynamic progress is achieved, and an objective and accurate myopathy prognosis evaluation tool is provided for clinic.

The present invention belongs to the field of genetic breeding technology, and specifically relates to a haplotype molecular marker affecting sheep loin muscle area traits and its application. The haplotype molecular marker includes <h2 style=";text-align:left;direction:ltr">LOC105603993 ‑SNP1~ <h2 style=";text-align:left;direction:ltr"> LOC105603993 SNP7 has a total of 7 sites, located at the 101bp position of the nucleotide sequences shown in SEQ ID NO.1 to SEQ ID NO.7. The haplotype molecular marker of the present invention can be used to identify the psoas muscle area trait of sheep. By selecting individuals with the haplotype combination of TTAAGGAACCTTCC as the father or mother, the psoas muscle area of ​​the offspring can be increased.

The present application belongs to the technical field of determination or detection method of enzyme, nucleic acid or microorganism, and relates to a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle and application thereof. The present application aims to provide a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle. To solve the technical problem, the present application provides a molecular marker obtained by genome-wide association (GWAS) analysis, the nucleic acid sequence of which is shown as SEQ ID NO:1, and there is an A>G nucleotide single base mutation at 6328556 of the pig genome 9th chromosome, which significantly affects the content of 2-acetyl-3-methylpyrazine in pig muscle. By detecting the genotype of the SNP site of the pig to be tested, early selection of the content of 2-acetyl-3-methylpyrazine in pig muscle can be realized.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

The application provides a large yellow croaker anti-flow trait associated SNP marker combination and breeding application, the combination is prepared from 13 SNP molecular marker combination probes for detecting the muscle elasticity trait of the large yellow croaker, the position of each SNP molecular marker on the chromosome, the changed allele of the site, and the reference gene information are shown in Table 1. Through the SNP marker combination of the application, the best large yellow croaker individual with anti-flow potential can be selected in advance in the breeding offspring, breeding and cultivation to the parent, without breeding and cultivation of a large number of offspring seedlings in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination of the application can solve the problem that the muscle elasticity of the large yellow croaker cannot be detected by non-lethal detection in the traditional breeding mode, and can ensure the smooth development of the large yellow croaker anti-flow trait breeding work.

The present invention belongs to the field of genetic breeding technology, and specifically relates to a haplotype molecular marker affecting the thickness of sheep loin muscle and its application. The haplotype molecular marker includes CELF4 ‑SNP1~ CELF4 ‑SNP2 has 2 sites; among them, CELF4 -SNP1 is located at the 101bp position of the nucleotide sequence shown in SEQ ID NO.1, where a G to C mutation occurs; CELF4 The gene position of SNP2 is 101 bp of the nucleotide sequence shown in SEQ ID NO. 2, where a mutation from G to C occurs. The haplotype molecular marker of the present invention can be used to identify the psoas muscle thickness trait in sheep. By selecting individuals with the haplotype combination of GGGG as the sire or dam, the psoas muscle thickness of the offspring can be increased.

Aspects of the disclosure relate to compositions and methods for treating Fibrodysplasia ossificans progressiva (FOP). The disclosure is based, in part, on muscle-targeting, chemically-modified interfering nucleic acids (e.g., siRNAs) that bind to and inhibit an ACVR1R206H allele or an Inhba allele of a subject.

The invention discloses an SNP molecular marker related to the cold resistance character of a TRPP gene of penaeus vannamei boone and application of the SNP molecular marker, and belongs to the technical field of penaeus vannamei boone breeding, the nucleotide sequence of the SNP molecular marker is shown as a sequence 1 in a sequence table, and the SNP molecular marker is remarkably related to the cold resistance character of the penaeus vannamei boone and can be used as a functional marker for the cold resistance of the penaeus vannamei boone. The method comprises the following steps: extracting genome DNA (deoxyribonucleic acid) of appendage muscle tissues of penaeus vannamei to be detected, performing PCR (polymerase chain reaction) amplification, sequencing obtained products, and screening individuals carrying dominant genotypes according to genotypes of different sites on the SNP molecular marker; the method is used for breeding the cold-resistant strain of the penaeus vannamei boone, so that the penaeus vannamei boone with excellent cold resistance can be quickly and accurately identified in the early stage, and support is provided for breeding new cold-resistant varieties of the penaeus vannamei boone.

The invention discloses an overexpression and interference lentiviral vector constructed based on miR-486-5p as well as a construction method and application of the overexpression and interference lentiviral vector. According to the lentiviral vector, an oligonucleotide sequence is constructed on multiple cloning sites of a plasmid skeleton, and lentivirus is used for packaging; the nucleotide sequence of the oligonucleotide is one selected from the group consisting of miR-486-5p-shRNA30, miR-486-5p-shRNA451, Anti-miR-486-5p TuD and Anti-miR-486-5p, and the nucleotide sequence of the oligonucleotide is one selected from the group consisting of miR-486-5p and Anti-miR-486-5p; and the plasmid skeleton contains a promoter and a plasmid skeleton of MCS multiple cloning sites. According to the present invention, the hairpin structure of pri-miR-451 is innovatively adopted as the skeleton, the AGO2 non-classical processing pathway and the miRNA cavernous body adsorption principle are combined to construct the regulation and control vector adapted to the miR-486 characteristics, and the core regulation and control effect is provided in the muscle cell development through the gene knockout or overexpression;

This application discloses a dynamic regulatory system for L-carnosine synthesis and its applications. The dynamic regulatory system includes a first expression unit containing a first growth-phase-dependent promoter, a second expression unit containing a second growth-phase-dependent promoter, and a third expression unit containing a third growth-phase-dependent promoter. This dynamic regulatory system can significantly increase L-carnosine production, demonstrating yield-enhancing effects on integrated genomes and different carnosine-producing strains.

The invention discloses an SNP molecular marker related to the cold resistance character of a Penaeus vannamei GPx gene and application thereof, and belongs to the technical field of Penaeus vannamei breeding, the nucleotide sequence of the SNP molecular marker is shown as a sequence 1 in a sequence table, the SNP molecular marker is remarkably related to the cold resistance character of Penaeus vannamei, and the SNP molecular marker can be used as a functional marker for the cold resistance of Penaeus vannamei. The method comprises the following steps: extracting genome DNA of appendage muscle tissues of penaeus vannamei to be detected, carrying out PCR amplification, sequencing the obtained product, screening individuals carrying dominant genotypes according to genotypes of different sites on the SNP molecular marker, and breeding the penaeus vannamei cold-resistant strain. Therefore, early, rapid and accurate identification of the penaeus vannamei with excellent cold resistance is realized, and effective technical support is provided for breeding of new cold-resistant varieties.

The application discloses a primer set for real-time fluorescent quantitative PCR detection of a pig muscle regulatory factor and application thereof, and belongs to the technical field of biology. The application provides specific primer pairs capable of detecting pig muscle regulatory factors MyoD and MyoG and reference primer pairs. cDNA is obtained by extracting sample RNA and performing reverse transcription, and real-time fluorescent PCR is performed by using the provided primers, so that the mRNA expression amount of the pig muscle regulatory factors MyoD and MyoG can be simultaneously detected quickly, specifically, sensitively and stably, and the application can be used for detecting the change in the expression level of the regulatory factors before and after the differentiation of pig muscle stem cells, so that the differentiation condition of pig breeding meat can be judged.

The present application relates to the technical field of genetic breeding, and particularly relates to application of a haplotype molecular marker related to sheep weight and muscle fiber diameter, wherein the haplotype molecular marker is 1) and 2): 1) a nucleotide sequence shown in SEQ ID NO. 1; the nucleotide at the 101bp site of the SEQ ID NO. 1 is T or C; 2) a nucleotide sequence shown in SEQ ID NO. 2; the nucleotide at the 101bp site of the SEQ ID NO. 2 is C or T. Through the significant SNP site related to weight, early identification of the muscle fiber diameter of live sheep is realized, which can be used for assisting breeding to reduce the muscle fiber diameter of offspring, has the characteristics of non-damage, high efficiency and precision, and can effectively shorten the breeding cycle and improve the growth performance of sheep and the efficiency of meat quality improvement.

The invention provides a beef cattle MSTN gene promoter function optimization design method based on deep learning and model adaptation. The beef cattle MSTN gene promoter function optimization design method comprises the following steps: taking RNA-seq expression quantity of an MSTN gene as a supervision label, and taking high-dimensional epigenetic signal prediction data obtained by inputting a promoter region sequence into a pre-trained Enformer deep learning model as an input feature; training a machine learning adapter model; when the prediction precision of the adapter model reaches a preset threshold value, determining the adapter model as a verification model; constructing a promoter variant sequence library of the MSTN gene by adopting a multi-strategy variant generation method; the promoter activity is verified by a reporter gene expression verification method aiming at the promoter sequence which ranks in the top in the variant sequence library. The invention also discloses an enhanced promoter with a sequence as shown in SEQ ID NO.4 and a weakened promoter with a sequence as shown in SEQ ID NO.2 or 3.

The application discloses a kind of for sheep MSTN And SOCS2 The sgRNA of gene cytosine base editing and application thereof, the sgRNA includes nucleotide such as any one or several of SEQ ID NO.1~4.This application is respectively designed sgRNA guide sequence for sheep MSTN And SOCS2 Gene, introduce stop codon in target site using CBE system, select sgRNA guide sequence and its combination from which can efficiently target sheep MSTN And SOCS2 Gene, carry out gene editing efficiency verification on cell, and successfully obtain MSTN And SOCS2 Double gene editing goat by prokaryotic embryo microinjection technology, and gene editing sheep shows muscle fiber thickening, and fast growth rate.This application establishes a set of efficient, precise double gene editing technology system, provides key breeding material and technical support for quickly cultivating muscle developed, fast growth meat sheep new variety.

The invention discloses an RNA co-immunoprecipitation kit for specific binding of prion protein in muscle cells and an RNA extraction method, and relates to the technical field of bioengineering. According to the present invention, the specific co-precipitation kit preparation and the specific extraction steps comprise cell lysis, cell lysis solution-antibody-magnetic bead incubation, protein digestion and RNA extraction, such that the high purity and the sufficient amount of the enriched RNA are successfully ensured so as to completely meet the strict requirements of the subsequent RNA-seq sequencing. The method overcomes the inherent defects of the existing RIP technology in the research of non-classical RNA binding proteins, realizes the optimization of the whole process from high-specificity enrichment to high-quality sequencing sample preparation through the integrated innovation of methodology and the kit, has the outstanding advantages of strong specificity, high sensitivity, good repeatability, simple operation and the like, and has a wide application prospect in the research of non-classical RNA binding proteins. And remarkable technical progress is achieved, and a positive application effect is generated.

The invention discloses a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method and a kit for simultaneously detecting IMNV (Immunodeficiency Virus), CMNV (Cytomegalovirus) and DIV1 (Deoxyribose Immunodeficiency Virus According to the invention, PCR primers and TaqMan probes for detecting IMNV, CMNV and DIV1 are respectively designed, and the nucleotide sequences of the PCR primers and TaqMan probes are shown as SEQ ID NO: 1-9. The primer and the probe can be used for simultaneously qualitatively and quantitatively detecting the infectious muscle necrosis virus, the secretly death nodavirus and the full-eye iridovirus 1 through a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method. According to the invention, common detection of RNA virus and DNA virus is realized for the first time, the kit has the characteristics of high efficiency and convenience, and the advantages of high sensitivity, high specificity and quantitability, and meanwhile, due to the addition of the molecular crowding agent, the detection efficiency and detection speed of medium and low-concentration virus load can be effectively improved, and the detection cost is reduced. The invention provides a convenient and reliable detection method and product for early monitoring, early warning and diagnosis of the three virus diseases of the penaeus vannamei boone.

The application belongs to bioengineering technology and particularly relates to a haplotype breeding marker related to pig feed utilization rate and meat production traits and application. The genetic marker is cloned from 19 sites of a SYISL gene promoter of a pig chromosome 8. Three pairs of primers are designed according to a pig genome sequence (NC_010450.4) published by NCBI database. There are 19 base substitutions in the amplified sequences of the three pairs of primers. The SNPs sites are typed by a direct sequencing technology, and the linkage disequilibrium analysis result shows that the 19 sites are all in a complete linkage disequilibrium state, and two haplotypes are generated by analysis. Correlation analysis shows that compared with other haplotype individuals, the H2-H2 combined haplotype individuals have thinner backfat thickness, larger eye muscle thickness, less average feed intake of 30-100 kg body weight and smaller feed-meat ratio. The application provides a new marker for pig meat production trait molecular breeding.

This invention discloses a targeted MSTN The sgRNA of the gene and its application in constructing a feline myostatin gene knockout model; the targeted MSTN The nucleotide sequence of the gene's sgRNA is shown in SEQ ID NO.1 and / or SEQ ID NO.2. This invention systematically establishes a CRISPR / Cas9 genome editing platform suitable for domestic cats for the first time and successfully constructs the world's first... MSTN The gene knockout live model in domestic cats represents a breakthrough in the technical barriers to functional gene research in domestic cats. It has been demonstrated that sgRNA is a highly efficient editing tool for the MSTN gene in domestic cats. The constructed... MSTN The gene knockout domestic cat model provides an unprecedented tool for in-depth research on muscle biology and muscle diseases in mammalian (cat) systems that are more closely related to humans. It fills the gap in large animal models in this field and has significant value for basic scientific research and translational medicine.

The invention discloses a method for creating fluorescent zebra fish based on an enhancer of a muscle specific gene. The invention provides a method for creating fluorescent zebrafish, which comprises the following steps: by taking an upstream promoter and an enhancer of a fmyhc2.2 gene as target sequences, amplifying the upstream promoter and the enhancer and seamlessly connecting the upstream promoter and the enhancer with a fluorescent protein carrier to form a recombinant plasmid, carrying out microinjection on a zebrafish embryo, selecting F0 generation individuals capable of driving fluorescent protein expression, and culturing until sexual maturity, thereby obtaining the fluorescent zebrafish. Selecting male and female individuals for selfing to obtain F1 generation, observing and selecting individuals with strong fluorescence from F1 generation embryos through a fluorescence microscope, and culturing the individuals with strong fluorescence to sexual maturity to obtain the transgenic zebrafish with remarkably discolored skin. The fluorescent zebra fish is created by utilizing the enhancer of the muscle specific gene, an innovative method is provided for creating a zebra fish ornamental strain, a support is provided for researching a regulation mechanism of zebra fish muscle related genes, and the fluorescent zebra fish has important scientific value and industrial application value.