78 results about "MUSCLE" patented technology

The invention relates to a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle and application of the molecular marker, belonging to the technical field of determination or inspection methods of enzymes, nucleic acids or microorganisms. The technical problem to be solved by the invention is to provide a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle. In order to solve the technical problem, the molecular marker provided by the invention is obtained through genome-wide association (GWAS) analysis, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the molecular marker has an Agt at the 6328556 position of a chromosome 9 of a pig genome; a nucleotide single base of G is mutated, and the mutation obviously affects the content of 2-acetyl-3-methylpyrazine in pig muscles. By detecting the genotype of a to-be-detected pig at the SNP site, early selection of the content of 2-acetyl-3-methylpyrazine in pig muscles can be realized.

The invention belongs to the technical field of gene detection, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker, a primer pair, a kit, a detection method and application of a g.64930 site of a PPARG (Peroxisome Peroxisome Activated Receptor Gene) gene of Anhui local variety cattle. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO.1 and SEQ ID NO.2, when the basic group at the 118th site of the 5'end of the molecular marker is G, the meat color brightness of beef is higher than that when the basic group at the site is T, and the muscle pH is lower than that when the basic group at the site is T. The molecular marker can be used as a candidate molecular marker for the quality character of the Anhui local variety cattle, is applied to population breeding of the Anhui local variety cattle, and is combined with production requirements for molecular-assisted breeding, so that a theoretical basis is conveniently provided for variety resource development and breeding of the Anhui local variety cattle.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention discloses a biological age assessment method, which comprises the following steps: acquiring a key protein index of a to-be-assessed person, and inputting an improved biological age assessment model I to obtain the biological age of the to-be-assessed person; the improved biological age assessment model I is constructed and obtained based on an elastic network regression algorithm by taking proteome data and body composition age of an individual as training data. The invention also discloses a construction method of the model, namely a two-step method. According to the method, the user is allowed to calculate the biological age according to the proteome data of the user, personalized monitoring of the muscle aging state is achieved, and the method has great guiding significance in the aspects of identifying individuals with premature aging of muscle functions and formulating personalized precise anti-aging schemes.

The invention relates to the technical field of nuclide probes, and discloses a targeted PARP nuclide probe and a preparation method and application thereof, and the targeted PARP nuclide probe comprises a radionuclide labeled compound as shown in formula I; the targeted PARP nuclide probe provided by the invention can be used as a diagnosis and treatment reagent to be applied to tumors with high PARP protein expression in human or animal bodies, and is especially suitable for tumor imaging and radionuclide treatment; animal experiments show that the nuclide probe has a high tumor / muscle uptake ratio and shows good application potential. Formula I.

The invention belongs to the technical field of genetic breeding, and particularly relates to a haplotype molecular marker influencing the sheep lumbar muscle thickness character and application, and the haplotype molecular marker comprises two sites CELF4-SNP1-CELF4-SNP2; wherein the position of the gene where CELF4-SNP1 is located is that mutation from G to C occurs at 101bp of a nucleotide sequence as shown in SEQ ID NO.1; the position of the gene where CELF4-SNP2 is located is that mutation from G to C occurs at 101bp of the nucleotide sequence as shown in SEQ ID NO.2. The haplotype molecular marker can be used for identifying the lumbar muscle thickness character of sheep, and the lumbar muscle thickness of offspring is increased by selecting an individual of which the haplotype combination is GGGG as a male parent or a female parent.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to porcine No.8 chromosome and muscle shear force as well as a detection method and application. The SNP marker is located on a pig 8 # chromosome, the site of the SNP marker is the nucleotide site of the pig 8 # chromosome rs332282301 of a reference sequence of an international pig genome version 11.1, the SNP marker has A / G polymorphism, and the SNP marker is significantly related to the pig muscle shear force. According to a primer pair for detecting the SNP marker, an upstream primer is SEQ ID NO: 2, and a downstream primer is SEQ ID NO: 3. The SNP marker provided by the invention is related to the tenderness of the pigs, the pigs with low muscle shearing force (better tenderness) can be screened by identifying the SNP marker, and the SNP marker has important economic benefits and social values for establishing pig groups with low muscle shearing force (better tenderness).

This application relates to the technical field of Yanhuang cattle breeding production, specifically to a molecular marker associated with the muscle fatty acid content of Yanhuang cattle and its application. This molecular marker includes a nucleotide sequence formed by a C>T mutation at position 20,849,921 bp on chromosome 20 of the bovine reference genome GCF_002263795.3. This molecular marker is closely associated with the muscle fatty acids of Yanhuang cattle and can be used in the breeding of high-quality Yanhuang cattle rich in high-quality fatty acids.

The invention described here contains a nucleic acid expression cassette comprising a transcriptional regulatory region operably linked to a nucleic acid sequence encoding a FKRP, an RNA transcript comprising a modified 5′ and / or 3′ untranslated region (UTR) that will be utilized to treat a variety of FKRP-mediated diseases.

The present application relates to the technical field of livestock feed, and particularly relates to a feed for improving muscle growth of broilers and application of a molecular marker (His-Thr-Gln-Glu) of the feed in cells, and provides a molecular marker for regulating animal skeletal muscle, and an amino acid sequence of the molecular marker is His-Thr-Gln-Glu, and the molecular marker can be applied to preparation of a product for regulating animal skeletal muscle as a target.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

Compositions and methods for modifying a muscle or muscle precursor cell genome are disclosed. Also provided are methods of treatment using the compositions.

The invention belongs to the technical field of genetic breeding, and particularly relates to a haplotype molecular marker influencing the lumbar muscle area character of sheep and application of the haplotype molecular marker. The haplotype molecular marker comprises seven loci LOC105603993-SNP1-LOC105603993-SNP7 which are located at the 101bp position of the nucleotide sequence shown in SEQ ID NO.1-SEQ ID NO.7. The haplotype molecular marker can be used for identifying the lumbar muscle area character of the sheep, and can be used for identifying the lumbar muscle area character of the sheep. An individual with a haplotype combination of TTAAGGAACCTTCC is selected as a male parent or a female parent, so that the lumbar muscle area of an offspring is increased.

The invention provides a method for constructing a mouse TGIF1 gene knockout cell line based on a CRISPR / Cas9 technology, and belongs to the technical field of gene editing. According to the invention, the CRISPR / Cas9 technology is adopted, the TGIF1 gene in the C2C12 cell is successfully knocked out, the C2C12 cell line with the knocked-out TGIF1 gene is established, the cell line is stable in heredity, and the cell morphology and proliferation are not obviously different from those of a control group cell. The method can be used for researching the function of the TGIF1 gene in mouse muscle development and revealing the action mechanism of the TGIF1 gene in muscle development regulation.

Provided herein are compositions and methods for treating or preventing dilated cardiomyopathy (DCM) in a subject in need thereof, for example, through correcting one or more point mutations in the RBM20 locus using CRISPR-associated base editing. TIFF2025538153000011.tif56128

The invention discloses application of a traceless gene editing cell line in research on influence of IGF-1 on cell proliferation and apoptosis, an interference molecule for inhibiting TM4SF4 expression and application, and relates to the technical field of biomedicine. Comprising the following steps: step 1, replacing an MSTN sequence with an IGF-1 sequence through a CRISPR / Cas9 editing technology; constructing an expression vector capable of expressing IGF-1; step 2, introducing the expression vector in the step 1 into a cell, carrying out amplification culture, extracting a genome of a cell line by using a genome extraction kit, carrying out PCR amplification by using a primer pair, recovering an amplification product, and carrying out sequencing identification; the monoclonal cell line which is identified to be correct is the IGF-1 gene editing cell line; and 3, researching the influence of IGF-1 on cell proliferation and apoptosis by adopting an IGF-1 gene editing cell line which is correctly identified. Regulation of IGF-1 on myoblast proliferation is researched on the basis of a traceless gene editing cell line, and a key regulation gene of a downstream signal channel after MSTN is replaced by IGF-1 is screened in combination with transcriptome sequencing, so that an IGF-1 mediated muscle growth molecular mechanism is clarified.

The application discloses a biological age evaluation method, comprising the following steps: obtaining a key protein index of a person to be evaluated, inputting an improved biological age evaluation model I, and obtaining the biological age of the person to be evaluated; the improved biological age evaluation model I is obtained based on an elastic network regression algorithm by taking proteomic data and body composition age of an individual as training data. The application also discloses a construction method of the model, i.e., a two-step method. The application allows a user to calculate the biological age according to the proteomic data, realizes personalized monitoring of muscle aging state, and has great guiding significance in identifying individuals with early muscle function decline and formulating personalized and accurate anti-aging schemes.

The invention provides a myopathy progress prediction method and device based on a spatio-temporal topology network, and the method comprises the steps: carrying out the image segmentation and quantification of muscle biopsy sections of a patient at a plurality of time points, constructing a static pathological topology network with a single muscle fiber as a node and a spatial adjacency relation as an edge, and enabling the node to comprise the multi-dimensional features of subtypes, forms, textures, cell nucleuses and the like; and forming time-space diagram data according to a time sequence. The data are input into a pre-trained space-time diagram neural network, a space coding layer aggregates neighborhood information through a diagram convolutional network to capture space interaction, a time coding layer learns a time sequence evolution rule through a recurrent neural network, and a decoding layer predicts a network state of a next time point. Pathological network sequences of multiple time points in the future are generated through iterative prediction, then key indexes are extracted to draw a quantitative prediction curve, a visual pathology heat map is generated, crossing from static pathology to dynamic progress is achieved, and an objective and accurate myopathy prognosis evaluation tool is provided for clinic.

The present invention belongs to the field of genetic breeding technology, and specifically relates to a haplotype molecular marker affecting sheep loin muscle area traits and its application. The haplotype molecular marker includes <h2 style=";text-align:left;direction:ltr">LOC105603993 ‑SNP1~ <h2 style=";text-align:left;direction:ltr"> LOC105603993 SNP7 has a total of 7 sites, located at the 101bp position of the nucleotide sequences shown in SEQ ID NO.1 to SEQ ID NO.7. The haplotype molecular marker of the present invention can be used to identify the psoas muscle area trait of sheep. By selecting individuals with the haplotype combination of TTAAGGAACCTTCC as the father or mother, the psoas muscle area of ​​the offspring can be increased.

Provided herein are oligonucleotides, trimeric peptides, and peptide-oligonucleotide-conjugates. Also provided herein are methods of treating a muscle disease in a subject in need thereof, comprising administering to the subject oligonucleotides, trimeric peptides, and peptide-oligonucleotide-conjugates described herein. A synthetic method provides for the generation of a library of cell-penetrating peptides conjugated to an antisense oligonucleotide, and a machine learning-based generator-predictor-optimizer loop for the generation of novel peptide sequences capable of enhanced delivery of oligonucleotide cargo from the library of conjugates.

The present application belongs to the technical field of determination or detection method of enzyme, nucleic acid or microorganism, and relates to a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle and application thereof. The present application aims to provide a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle. To solve the technical problem, the present application provides a molecular marker obtained by genome-wide association (GWAS) analysis, the nucleic acid sequence of which is shown as SEQ ID NO:1, and there is an A>G nucleotide single base mutation at 6328556 of the pig genome 9th chromosome, which significantly affects the content of 2-acetyl-3-methylpyrazine in pig muscle. By detecting the genotype of the SNP site of the pig to be tested, early selection of the content of 2-acetyl-3-methylpyrazine in pig muscle can be realized.

The invention belongs to but not limited to the technical field of gene engineering, and discloses a preparation method and application of label-free lateolabrax japonicus FGF2 recombinant protein. The lateolabrax japonicus FGF2 recombinant protein comprises an FGF2 amino acid sequence; wherein the amino acid sequence of the FGF2 recombinant protein with the His tag is as shown in SEQ ID NO: 1; the DNA sequence of the coding gene is as shown in SEQ ID NO: 3; the amino acid sequence of the FGF2 recombinant protein is as shown in SEQ ID NO: 2. The prokaryotic expression vector pET-His-FGF2 of the lateolabrax japonicus FGF2 recombinant protein is constructed for the first time, the FGF2 recombinant protein consistent with the lateolabrax japonicus FGF2 in amino acid sequence is successfully obtained through His tag affinity purification and TEV enzyme digestion tag technologies, and the recombinant protein has wide application prospects in lateolabrax japonicus muscle development and lipid metabolism regulation and control research.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

The present invention relates to regulatory nucleic acid sequences, in particular muscle-specific promoters, elements thereof, and other such nucleic acid sequences, that are capable of enhancing muscle-specific expression of genes. The invention also relates to expression constructs, vectors and cells comprising such muscle-specific regulatory nucleic acid sequences, and to methods of their use. The regulatory nucleic acid sequences are of particular utility for gene therapy applications, but also find utility in other areas such as bioprocessing and biotechnology.

The application provides a large yellow croaker anti-flow trait associated SNP marker combination and breeding application, the combination is prepared from 13 SNP molecular marker combination probes for detecting the muscle elasticity trait of the large yellow croaker, the position of each SNP molecular marker on the chromosome, the changed allele of the site, and the reference gene information are shown in Table 1. Through the SNP marker combination of the application, the best large yellow croaker individual with anti-flow potential can be selected in advance in the breeding offspring, breeding and cultivation to the parent, without breeding and cultivation of a large number of offspring seedlings in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination of the application can solve the problem that the muscle elasticity of the large yellow croaker cannot be detected by non-lethal detection in the traditional breeding mode, and can ensure the smooth development of the large yellow croaker anti-flow trait breeding work.

The present invention belongs to the field of genetic breeding technology, and specifically relates to a haplotype molecular marker affecting the thickness of sheep loin muscle and its application. The haplotype molecular marker includes CELF4 ‑SNP1~ CELF4 ‑SNP2 has 2 sites; among them, CELF4 -SNP1 is located at the 101bp position of the nucleotide sequence shown in SEQ ID NO.1, where a G to C mutation occurs; CELF4 The gene position of SNP2 is 101 bp of the nucleotide sequence shown in SEQ ID NO. 2, where a mutation from G to C occurs. The haplotype molecular marker of the present invention can be used to identify the psoas muscle thickness trait in sheep. By selecting individuals with the haplotype combination of GGGG as the sire or dam, the psoas muscle thickness of the offspring can be increased.

Aspects of the disclosure relate to compositions and methods for treating Fibrodysplasia ossificans progressiva (FOP). The disclosure is based, in part, on muscle-targeting, chemically-modified interfering nucleic acids (e.g., siRNAs) that bind to and inhibit an ACVR1R206H allele or an Inhba allele of a subject.

The invention discloses an SNP molecular marker related to the cold resistance character of a TRPP gene of penaeus vannamei boone and application of the SNP molecular marker, and belongs to the technical field of penaeus vannamei boone breeding, the nucleotide sequence of the SNP molecular marker is shown as a sequence 1 in a sequence table, and the SNP molecular marker is remarkably related to the cold resistance character of the penaeus vannamei boone and can be used as a functional marker for the cold resistance of the penaeus vannamei boone. The method comprises the following steps: extracting genome DNA (deoxyribonucleic acid) of appendage muscle tissues of penaeus vannamei to be detected, performing PCR (polymerase chain reaction) amplification, sequencing obtained products, and screening individuals carrying dominant genotypes according to genotypes of different sites on the SNP molecular marker; the method is used for breeding the cold-resistant strain of the penaeus vannamei boone, so that the penaeus vannamei boone with excellent cold resistance can be quickly and accurately identified in the early stage, and support is provided for breeding new cold-resistant varieties of the penaeus vannamei boone.

The invention discloses an overexpression and interference lentiviral vector constructed based on miR-486-5p as well as a construction method and application of the overexpression and interference lentiviral vector. According to the lentiviral vector, an oligonucleotide sequence is constructed on multiple cloning sites of a plasmid skeleton, and lentivirus is used for packaging; the nucleotide sequence of the oligonucleotide is one selected from the group consisting of miR-486-5p-shRNA30, miR-486-5p-shRNA451, Anti-miR-486-5p TuD and Anti-miR-486-5p, and the nucleotide sequence of the oligonucleotide is one selected from the group consisting of miR-486-5p and Anti-miR-486-5p; and the plasmid skeleton contains a promoter and a plasmid skeleton of MCS multiple cloning sites. According to the present invention, the hairpin structure of pri-miR-451 is innovatively adopted as the skeleton, the AGO2 non-classical processing pathway and the miRNA cavernous body adsorption principle are combined to construct the regulation and control vector adapted to the miR-486 characteristics, and the core regulation and control effect is provided in the muscle cell development through the gene knockout or overexpression;

This application discloses a dynamic regulatory system for L-carnosine synthesis and its applications. The dynamic regulatory system includes a first expression unit containing a first growth-phase-dependent promoter, a second expression unit containing a second growth-phase-dependent promoter, and a third expression unit containing a third growth-phase-dependent promoter. This dynamic regulatory system can significantly increase L-carnosine production, demonstrating yield-enhancing effects on integrated genomes and different carnosine-producing strains.