91 results about "MUSCLE" patented technology

Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) / CRISPR-associated (Cas) 9-based system related compositions and methods of using said CRISPR / Cas9-based system related compositions for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using said compositions for altering gene expression and genome engineering in muscle, such as skeletal muscle and cardiac muscle.

The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

The invention discloses a nucleotide sequence or an amino acid sequence of a PHKG1 (phosphorylase kinase, gamma 1) gene located on pig chromosome 3, wherein a mutation site of the PHKG1 gene is the C-A mutual mutation of a mononucleotide at the 8118th site of a sequence shown in SEQ ID NO: 1; when C is mutated to A, a normal transcript generated by the PHKG1 gene in a transcription process deletes abnormal transcripts of 32 bp fragments, and the deleted 32 bp fragments are located at the 8123rd-8154th base of 5' end of the sequence shown in SEQ ID NO: 1; on the basis, disadvantageous genotype individuals are eliminated by selecting advantageous genotype individual reserve breeding pigs, so that population meat quality traits can be remarkably improved, especially, muscle glycogen content can be reduced, drip loss and processing loss can be reduced, and pH value and intramuscular fat content can be increased, thus the pig meat quality is improved.

The invention discloses a fluorescence quantitative PCR detection method for type and composition of yak muscle fibers. According to the fluorescence quantitative PCR detection method, yak muscle tissues are taken for total RNA extraction, the RNA purity and concentration are detected, the total RNA is subjected to inverse transcription to generate cDNA, then quality detection is carried out by taking GAPDH genes of yak as internal genes, and then a special detection primer is adopted for carrying out fluorescence quantitative PCR reaction, meanwhile, the GAPDH genes of yak are taken as reference genes for statistic analysis. The detection method and the detection primer provided by the invention are more accurate and more reliable than histochemistry evaluation; with the molecule quantitative classification method, accurate quantitative and analysis can be carried out on the type composition of the various muscle fibers in muscles at different parts of the yak, so that a technological base is laid for further study on the relevance between the muscle fibers and the meat quality trait of the yak, and meanwhile, compared with other methods, the fluorescence quantitative PCR detection method has the advantages of low workload and low cost, and high efficiency.

The invention discloses a chicken muscle fiber-type property-related gene molecule marker and a use thereof. A 646th nucleotide G-to-A mutational site of a PGC-1 alpha gene exon is used as a molecule marker, the molecule marker has a nucleotide sequence shown in the formula of SEQ ID NO. 1, and at 97th bp of the nucleotide sequence shown in the formula of SEQ ID NO. 1, G97-A97 base mutation is produced. The molecule marker provides a fast, simple and low cost gene analysis method for breeding of chicken with chicken muscle fiber-type properties, is conducive to screening of chicken with high slow muscle fiber content and good meat quality and can guide molecule marker-assistant breeding of chicken with chicken muscle fiber-type properties.

This invention relates to endostatin small molecular polypeptide, its coding nucleotide sequence and complementary strand. The endostatin small molecular polypeptide has 30 amino acid residues corresponding to a nucleotide sequence with a full length of 90 bp and a complementary strand with a length of 88 bp. The endostatin small molecular polypeptide avoids the defects of the current endostatin such as high molecular weight, limit to subcutaneous injection and intramuscular injection, large dosage and unsafety in intravenous injection. The endostatin small molecular polypeptide has such advantages as low molecular weight and no medicine accumulation, and is suitable for intravenous, intramuscular and subcutaneous injection. The coding nucleotide sequence and complementary strand can be synthesized by an automatic synthesizer, and used to manufacture endistatin in large scale by genetic engineering after recombination and transformation.

The invention discloses a method for quickly detecting RFLP (restriction fragment length polymorphism) of SNP (single nucleotide polymorphism) of a common ox SIRT1 (silent information regulator 1) gene promotor region. The method comprises the following steps of: taking whole genome DNA (deoxyribonucleic acid) of common ox blood as a template and a primer pair P (F and R) as a primer; performing PCR (polymerase chain reaction) amplification on the common ox SIRT1 gene promotor region; digesting a PCR amplification product by using restriction endonuclease SmaI; performing agarose gel electrophoresis on segments after enzyme digestion; and identifying the SNP of the common ox SIRT1 promotor region according to a result of the agarose gel electrophoresis. Because important functions including muscle differentiation, lipogenesis and the like related to the SIRT1 are closely related to common ox meat quality characteristics, and the SNP can significantly affect the promotor activity of the SIRT1 gene and is related to various important economic characteristics of the common ox, the detection method provided by the invention lays a foundation for the establishment of the relationship between the SNP and growth characteristics of the SIRT1 gene, and can be used for marker assisted selection of the growth characteristics of the Chinese common ox so as to facilitate rapid establishment of common ox populations with excellent genetic resources.

The invention discloses an RT-LAMP detection kit and a detection method of infectious myonecrosis viruses, wherein the kit consists of a mixed solution of outer primers F3 and B3 and inner primers FIP and BIP; and nucleotide sequences of the primers F3, B3, FIP and BIP are separately shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. According to the detection method, an RT-LAMP detection system is used for detecting whether a sample contains the infectious myonecrosis viruses or not. The method is simple and convenient to operate and is relatively low in requirement on experimental instruments, and the method can be completed in a normal constant-temperature water bath kettle within 60min; moreover, by virtue of a method of adding a dye to a final product and by directly observing a result with naked eyes, the method can rapidly and accurately identify the infectious myonecrosis viruses; and the method has a broad popularization and application prospect in prawn aquaculture.

The invention relates to the field of biological engineering, in particular to a method for predicting a sheep eye muscle property by single nucleotide polymorphism (SNP). Polymerase chain reaction (PCR) amplification is performed on the total deoxyribonucleic acid (DNA) of sheep by primers shown as SEQ ID No.2 and SEQ ID No.3, the SNP of a PCR amplification product is detected, the 73rd basic group at the 5' end of SEQ ID No.1 in a sequence table is determined as G or A and an individual with a G allelic gene has a large eye muscle area and a large eye muscle width. Due to the detection of a polymorphic site, a new material is provided for molecular breeding and scientific basis is provided for the marker-assisted selection of the sheep eye muscle property.

The invention relates to a method for increasing the activity of a myogenin (MyoG) gene promoter, and belongs to the technical field of genetic engineering. The method for increasing the activity of the myogenin (MyoG) gene promoter is characterized in that a deletion fragment which is 373 bp in length and has higher muscle specific promoter activity, namely pGL3-MyoGpro373, is obtained by adopting a method for analyzing the activity of a promoter deletion fragment by means of cloning a nucleotide sequence, which is at the 5' end control region of the MyoG gene and is 2125 bp in length; a promoter element therein having the SP1 positive regulation effect is cloned; the two fragments are connected in series, so that an expression carrier containing two copying numbers of promoter regulatory elements is constructed; the expression carrier is called as pGL3-MyoGpro373-double, namely a promoter sequence having double characteristics; and the base composition of the expression carrier is represented by Seq ID No:2.

The invention belongs to the technical field of pig molecular marker preparation and particularly relates to a genetic marker using pig CKM (creatine kinase muscle) 5' flanking promoter segments as pig carcass traits and application. The genetic marker has the nucleotide sequence shown as SEQ ID NO (sequencer identifier number):1 and SEQ ID NO:2 in a sequence table. The genetic marker is characterized in that A / G and G / A mutations respectively exist at the 82bp part of the sequence, and the polymorphism of DraIII-RFLP is caused. The genetic marker is used for carrying out pig carcass traits correlation analysis on large white pig and meishan pig F2 generations, and the expression quantity of CKM in muscular tissues of large white pigs and meishan pigs containing different genotypes is detected. The invention also discloses a parting detection method of the genetic marker, and the novel genetic marker and the detection method are provided for the pig carcass traits molecular marker auxiliary selection.