6168 results about "Nucleotide sequencing" patented technology

The invention discloses an AsMYB19 gene and application thereof, and relates to the technical field of biology, the nucleotide sequence of the AsMYB19 gene is shown as SEQ ID NO.1, the AsMYB19 gene can regulate and control the tiller number and the yield of oat, gene resources and strategies are provided for oat breeding and improvement of oat germplasm resources, the breeding speed is increased, and the breeding efficiency is improved.

The invention discloses an AsSN1 gene and application, and relates to the technical field of biology. The nucleotide sequence of the AsSN1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the AsSN1 protein is as shown in SEQ ID NO.2. The AsSN1 gene and the protein provided by the invention can regulate and control the spikelet number of the oats, further regulate and control the yield of the oats, provide important support for oat breeding and germplasm resource improvement, and have important significance for meeting the development requirements of animal husbandry.

The invention discloses a hemoglobin-resistant Taq DNA polymerase mutant and a construction method thereof, and relates to the field of biology, and the hemoglobin-resistant Taq DNA polymerase mutant is characterized in that a nucleotide sequence for coding the Taq DNA polymerase is shown as SEQ ID NO.1, and the mutant comprises at least one mutation or all mutations selected from S623D and E721A sites. On the basis of natural Taq DNA polymerase, the molecular structure of the Taq DNA polymerase is modified through rational design and combination with a site-specific mutagenesis biotechnology, so that the Taq DNA polymerase mutant is more suitable for PCR amplification of samples containing hemoglobin and the like than a wild type, and the result judgment accuracy of a sample containing a hemoglobin inhibitor is more facilitated.

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention provides a production strain of an antibiotic-free plasmid, the production strain is a gene editing strain of a PIR strain and is named as PIR1-WN:: 0636 or PIR1-PR: 0636, the production strain contains a nucleotide sequence for coding toxin protein and the antibiotic-free plasmid, and the antibiotic-free plasmid contains a nucleotide sequence for coding antitoxin protein; and preferably, the replicon DNA element of the nonreactive plasmid is R6K-gamma. The toxin protein gene of the production strain disclosed by the invention can be stably passaged, has lethality after being induced and can be used for plasmid screening; according to the invention, the positive rate of transforming the nonreactive plasmid into the PIR1-WN:: 0636 strain is more than 80%, and stable production of the plasmid with a high superhelix ratio can be realized.

The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1 and application thereof, and relates to the technical field of biology. The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1. The nucleotide sequence of the enhancer RNA molecule MZGAe1 is as shown in SEQ ID NO: 1; meanwhile, the invention further provides a specific sgRNA sequence for activating the molecule in a targeted manner and a recombinant vector of the specific sgRNA sequence. Expression of endogenous MZGAe1 of cells is specifically activated by adopting a CRISPR activation technology, and conversion of mouse embryonic stem cells to bicellular-like cells can be efficiently promoted. By providing a brand-new endogenous targeted MZGAe1 accurate activation tool, the efficient transformation of wild mouse embryonic stem cells to bicellular cells can be realized only under the condition of endogenous activation of single enhancer RNA molecule MZGAe1, the proportion is at least increased by 5%, the expression of a totipotent marker gene ZSCAN4 is activated, and the expression of the totipotent marker gene ZSCAN4 is promoted. And an efficient and specific brand-new research tool and scheme are provided for researching zygote genome activation, embryonic development early events and cell reprogramming.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

The invention provides a nucleic acid aptamer for specific recognition of morphine and application of the nucleic acid aptamer, and belongs to the technical field of biosensing and detection. The nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO: 1. The screening method is based on a Capture-SELEX technology and comprises the key steps that streptavidin magnetic beads are used for fixing an ssDNA library, estradiol, deabietic acid and totarol are introduced to serve as reverse screening substances so as to remove non-specific sequences, and finally the high-specificity aptamer is obtained through high-throughput sequencing and affinity determination. The dissociation constant of the aptamer and morphine is 127.31 nM, and the aptamer shows high affinity and high specificity. The invention further relates to application of the aptamer in preparation of a sensor and a kit for detecting morphine, and a new technical means is provided for rapid detection of morphine.

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention provides a fluorescent protein selection marker staygold-ynaMr gene and application thereof, and belongs to the technical field of biological engineering. The method comprises the following steps: replacing codons of L-isoleucine in nucleotide sequences of a fluorescent protein gene staygold and a gene ynaM by using a rare codon ATA to respectively obtain the fluorescent protein gene staygold after codon replacement and the gene ynaMr after codon replacement, and connecting the two segments of genes after codon replacement by using a flexible protein peptide, and the fluorescent protein selection marker staygold-ynaMr gene is obtained. Experiments prove that the gene can obviously improve the screening efficiency of L-isoleucine high-yield strains.

The invention relates to a product of an SNP (Single Nucleotide Polymorphism) molecular marker related to sow litter size character detection and application of the product. The SNP molecular marker comprises Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt The nucleotide sequence of the gene is shown as SEQ ID No.1, and the nucleotide sequence of the gene is shown as SEQ ID No.1. The product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The SNP molecular marker provided by the invention can be used for breeding, breeding and evaluating multi-variety sows with high reproductive performance.

The invention relates to a mulberry MaBEH1 / 2 gene for improving plant salt tolerance and application thereof, and belongs to the technical field of plant genetic engineering and biology. Wherein the coding nucleotide sequence of the mulberry MaBEH1 / 2 gene for regulating and controlling the salt tolerance of the woody plant is shown as a sequence 3; and the amino acid sequence of the MaBEH1 / 2 expression protein is shown as a sequence 4. According to the invention, MaBEH1 / 2 is transferred into 84K poplars to obtain MaBEH1 / 2-OE transgenic poplars; compared with a non-transgenic poplar, the salt stress tolerance of the MaBEH1 / 2-OE transgenic poplar can be remarkably enhanced. The invention provides key gene resources and technical support for genetic improvement of salt-tolerant forest varieties.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention relates to the technical field of biological medicines, discloses application of circPDK1 protection, and particularly relates to application of a reagent for specific detection of circPDK1 in preparation of products for detection of esophageal cancer or prognosis evaluation of esophageal cancer. According to the application of the protected circular RNA in preparation of the esophageal cancer detection product, the circular RNA is circPDK1, and the nucleotide sequence of the circular RNA is shown as SEQ ID NO: 1. Experiments prove that circPDK1 is highly expressed in esophageal cancer tissues, growth, proliferation and migration of esophageal cancer cells are promoted, occurrence of esophageal cancer is promoted, knock-down of the circular RNA can significantly inhibit growth, proliferation and migration of esophageal cancer tumors, and the average survival time of esophageal cancer patients with low circPDK1 expression is longer than that of esophageal cancer patients with high circPDK1 expression. And a new research direction is provided for esophageal cancer detection, treatment and prognosis.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The invention discloses a rice salt stress resistant gene OsHAK11 coding protein and application thereof, and belongs to the field of plant genetic engineering. According to the method, a rice OsHAK11 gene (the nucleotide sequence is shown as SEQ ID NO.1) is knocked out through a CRISPR / Cas9 gene editing technology, and a mutant with significantly enhanced salt tolerance is obtained. The gene editing vector pEGCas9Pubi-B-OsHAK11 contains sgRNA of a region as shown in a target SEQ ID NO.4, a rice receptor material is transformed through agrobacterium tumefaciens mediation, and a plant with the OsHAK11 gene subjected to frame shift mutation is obtained through screening. A salt stress experiment shows that the survival rate of the mutant oshak11 is obviously higher than that of a wild type. The invention provides a new gene resource and an efficient technical means for salt-tolerant breeding of rice.

The invention provides a double-target degradation molecule based on a functionalized nucleic acid connexon and application thereof, and relates to the technical field of biological medicine, the double-target degradation molecule comprises an E3 ubiquitin ligase ligand at one end, a first target protein ligand at the other end, and the functionalized nucleic acid connexon located between the E3 ubiquitin ligase ligand and the first target protein ligand; the functionalized nucleic acid linker is a nucleotide sequence capable of specifically recognizing and combining a second target protein or a coding gene thereof, so that the protein level degradation of the first target protein and the nucleic acid level or expression level inhibition of the second target protein / gene are realized in the same molecule. Functionalized nucleic acid and a PROTAC strategy are organically combined, single-molecule double-target collaborative intervention is achieved, targeting efficiency and treatment potential are improved, higher flexibility and expandability are provided in synthesis and design, and a new molecular platform and technical route are provided for multi-target accurate treatment.

This invention discloses a milk protein containing composition comprising: at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence coded by nucleotide sequence which is at least 80% similar to sequences selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

Disclosed in the present invention are an HLA-A*11-targeted liver cancer mRNA vaccine, and a preparation method therefor and a use thereof. The mRNA vaccine of the present invention is an mRNA vaccine designed on the basis of the HLA-A*11:01 typing of a patient and having high-coverage and high-immunogenic tumor neoantigens, and is formed by transcribing DNA having a nucleotide sequence shown in SEQ ID NO: 32 to form an mRNA, and encapsulating the mRNA in lipid nanoparticles.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention discloses a nucleic acid aptamer OUC-Fx11-5T of fucoxanthin and application of the nucleic acid aptamer OUC-Fx11-5T, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ ID NO. 19. The nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine has a nucleotide sequence as shown in SEQ The invention further discloses the application of the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthin in identification or detection of the fucoxanthin. According to the nucleic acid aptamer OUC-Fx11-5T of the fucoxanthine, the affinity and dissociation constant of the nucleic acid aptamer OUC-Fx11-5T and the fucoxanthine is determined to be 68.2 nM through an isothermal titer thermal method, the nucleic acid aptamer OUC-Fx11-5T has no obvious affinity to structural analogues of the fucoxanthine such as violaxanthin, neoxanthin, zeaxanthin and beta-carotene, has high affinity and good specificity to the fucoxanthine, and can be used for preparing the fucoxanthine aptamer OUC-Fx11-5T of the fucoxanthine. The method can be used for identification or detection of fucoxanthine.

The invention relates to the field of biological detection, in particular to a monoclonal antibody combination for detecting human IL-6 protein and application. The combination comprises monoclonal antibodies 3A10 and 2G1, and amino acid sequences of complementary determining regions of variable regions of light and heavy chains of the monoclonal antibodies 3A10 and 2G1 are respectively shown as SEQ ID NO.1-12. The invention defines a complete variable region sequence and a coding nucleotide sequence of the antibody. The combination specifically recognizes human IL-6 recombinant and natural proteins, and has no cross reaction with IL-11. A double-antibody sandwich ELISA kit constructed based on the combination takes 3A10 as a coating antibody and 2G1 as a labeled antibody, and has high sensitivity and high specificity. The problems that an existing reagent is insufficient in specificity and low in sensitivity are solved, a reliable core raw material is provided for IL-6 standardized detection, and the method is suitable for preparation of a kit, a test strip and an antibody chip.

The invention belongs to the technical field of synthetic biology, and particularly relates to low-immunogenicity recombinant Iamp with high stability. The invention relates to III fusion type collagen as well as a preparation method and application thereof. The invention discloses a method for preparing recombinant Iamp; the amino acid sequence of the III fusion type collagen and the nucleotide sequence of the coding gene of the III fusion type collagen. According to the invention, the recombinant Iamp with high stability and low immunogenicity is prepared by an engineering bacterium fermentation method; and III, a fusion type collagen. The result of the embodiment shows that the recombinant Iamp provided by the invention; iII, a fusion type collagen (colIamp); and the III-1 has relatively good thermal stability, long-term stability and degradation resistance. Animal experiments prove that the recombinant Iamp of the invention; iII, a fusion type collagen (colIamp); the III-1 has relatively low immunogenicity, and does not cause immune balance disorder of animals. In addition, the hydrogel also has the characteristics of high biocompatibility and good safety, and has no influence on the overall health condition of animals.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.