982 results about "Nucleotide sequencing" patented technology

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.

This invention discloses a method for preventing attacks by the star longhorn beetle. AcAttacin The dsRNA of the gene and its applications, as described AcAttacin The nucleotide sequence is shown in SEQ ID NO: 1. A *D. spp.* longhorn beetle ds. was successfully constructed using injection and feeding methods. AcAttacin RNA interference (RNAi) system, for AcAttacin The expression of the beetle exhibits highly efficient inhibition; using this system, the longhorn beetle... AcAttacin The combined application of this insect biocontrol bacterium, *Metarhizium anisopliae*, significantly enhances the control efficacy of *Metarhizium anisopliae* against the Asian longhorn beetle. This invention not only lays a solid theoretical foundation for the development of green, environmentally friendly, precise, and highly effective immunosuppressants for the Asian longhorn beetle, but also opens up a new strategic path for my country to achieve its green and sustainable development goals for the Asian longhorn beetle. It has extremely important significance and broad application prospects in ecological protection and forestry production.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The application provides a CPA primer probe set, a kit, a detection method and application for rapidly detecting Candidatus Liberibacter aurantii in the field, and belongs to the technical field of molecular detection.The application provides a CPA primer probe set for detecting Candidatus Liberibacter aurantii, which comprises a primer set and a probe HLB-P; the primer set comprises HLB-FB, HLB-AP, HLB-OP, HLB-IP, HLB-CPR and HLB-RB, and the nucleotide sequences thereof are shown in SEQ ID NO:1 to SEQ ID NO:6 in sequence; and the nucleotide sequence of the probe HLB-P is shown in SEQ ID NO:7. The application further provides a kit, a device and a detection method for detecting Candidatus Liberibacter aurantii. The application provides a reliable technical means for diagnosing different tissues of Candidatus Liberibacter aurantii, and lays an important technical foundation for real-time monitoring of field diseases and development of prevention and control strategies.

The application discloses application of a CALD1 gene SNP molecular marker in evaluation of chicken feed conversion rate traits and belongs to the technical field of chicken genetic breeding. The SNP molecular marker is located at the 401th nucleotide sequence shown in SEQ ID NO. 1, the 62383600th site of the first chromosome of a chicken, the RS number is rs732824512, and the base polymorphism is A or G. The polymorphic site is significantly related to the feed conversion rate traits of the chicken, the SNP molecular marker genotypes from high to low are GG, AG and AA in turn, the feed utilization performance of the chicken in the fast growth period can be more accurately judged, the chicken feed conversion rate traits can be early screened, the screening cost can be greatly reduced, the genetic breeding process can be accelerated, the breeding efficiency can be effectively improved, and the application has high economic application value and scientific research value.

Provided are small-fragment RNA derived from a circular RNA and the use thereof in the treatment of diabetic vascular diseases. The nucleotide sequence of the small-fragment RNA is CUAGCCGAAUGUUAAAAAAU. The provided small-fragment RNA has the effects of well protecting and maintaining the biological functions of vascular endothelial cells, has good structural stability, has a long half-life in vivo, and has the advantages of low effective concentration, high biological safety, etc. The provided small-fragment RNA has good application potentials in the field of clinical nucleic acid drug development, and provides a new idea for the treatment of chronic progressive vascular lesions caused by diabetes.

The application discloses a PARMS molecular marker significantly related to the tryptophan content of corn root systems and application thereof, the nucleotide sequence of 150 bp upstream and downstream of the molecular marker is shown as SEQ ID NO. 6, and the primer group nucleotide sequence for detecting the PARMS molecular marker is shown as SEQ ID NO. 1-SEQ ID NO. 3. ZmASB1 The application successfully develops a molecular marker based on the natural variation of the 5'UTR region of the (Zm00001eb080510) gene, the marker is significantly related to the tryptophan content of corn root systems, can accurately, efficiently and stably distinguish the InDel + genotype and the InDel ‑ genotype. The molecular marker can be widely applied to the tryptophan content of corn root system germplasm resources and molecular marker assisted breeding, and provides important technical support and theoretical basis for cultivating new corn varieties with high yield, stable yield and drought resistance.

This invention proposes a molecular marker for *Lactobacillus japonicus*, which was deposited on February 7, 2023, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 26505. The molecular marker comprises a nucleotide sequence selected from one of the following: (1) the nucleotide sequence shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99%, or higher homology to the nucleotide sequence shown in SEQ ID NO: 24; or (3) a nucleotide sequence having one or more nucleotide substitutions, deletions, or insertions in the nucleotide sequence shown in SEQ ID NO: 24. Using the molecular marker of this invention, not only can the target *Lactobacillus japonicus* be screened quickly and easily, but it can also highly specifically identify the target *Lactobacillus japonicus*, eliminating interference from other strains.

The application discloses a nucleic acid aptamer specifically combined with a small molecule of paroxetine hydrochloride and application thereof, and relates to a nucleotide sequence shown in SEQ ID No. 1, or a nucleotide sequence with high homology to the nucleotide sequence shown in SEQ ID No. 1 and capable of being specifically combined with a small molecule of paroxetine hydrochloride, or a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID No. 1 and capable of being specifically combined with a small molecule of paroxetine hydrochloride. The nucleic acid aptamer specifically combined with a small molecule of paroxetine hydrochloride has high affinity, high specificity, low molecular weight, easy modification, easy preservation and labeling.

The disclosure relates to novel soybean plants, plant parts, and nucleotide sequences in soybean plants comprising an amorphic allele of a RPF1 gene, along with methods of using and making the same.

The application discloses an Arabidopsis thaliana shade-avoidance regulation microprotein gene AtRAD-Like, an encoded protein and application thereof, relates to the technical field of genetic engineering, and the nucleotide sequence of the gene AtRAD-Like is shown in SEQ ID NO.1; and the amino acid sequence of the protein encoded by the gene AtRAD-Like is shown in SEQ ID NO.2. The application is characterized in that the Bar marker gene is used to impart herbicide resistance to a transformation receptor for screening and Western Blot is used to detect the expression of the AtRAD-Like-4MYC fusion protein, so that positive seedlings can be accurately and quickly detected, and phenotype analysis is further conducted on the AtRAD-Like gene overexpression transgenic line, it is found that the AtRAD-Like gene has a negative regulation effect on the hypocotyl length of plants in a shade environment, the finding provides a research basis for developing a new shade-tolerant plant, and has important significance.

This invention discloses the application of knocking down or knocking out the OsABA8ox2 protein or its encoding gene in improving the panicle germination resistance of rice seeds, belonging to the fields of biotechnology and plant genetic engineering technology. OsABA8ox2 The amino acid sequence of the protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes CRISPR / Cas9 technology to directionally edit the target site sequence in this gene, as shown in SEQ ID NO.3, successfully obtaining rice nucleotide sequences as shown in SEQ ID NO.8, SEQ ID NO.9, and SEQ ID NO.10. OsABA8ox2 The mutant, to a certain extent, enhances the dormancy of rice seeds, effectively reducing the germination rate of rice panicles. The gene resources and gene editing technology provided by this invention offer an important technical approach for rapidly improving or creating rice varieties with significantly enhanced panicle germination resistance in agricultural production, demonstrating significant application value in production practice.

This invention relates to a cytochrome P450 oxidase STO1, its encoding gene, and its applications. This invention is the first to clone and identify a cytochrome P450 oxidase gene, STO1, from potato responsible for catalyzing the synthesis of spirogelothoraxone, and to determine the nucleotide sequence and translated amino acid sequence of this gene. This invention provides expression vectors, recombinant microorganisms, or transgenic cell lines containing this gene. This invention constructs an engineered Saccharomyces cerevisiae strain for producing spirogelothoraxone, in which the strong promoter ERG9 is replaced by the weak promoter HXT1 in the strain's genome, and the STO1, AtCPR, tHMG1, and STS genes are integrated. This invention also provides a method for constructing this engineered strain and a method for producing spirogelothoraxone using this engineered strain, which can be used for the preparation of spirogelothoraxone.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

This invention discloses a phosphorylated diamine morpholino oligonucleotide drug that regulates PAPOLA expression and its application, belonging to the field of biomedical technology. The nucleotide sequence of the drug is shown in SEQ ID NO:1, targeting the coding region of human PAPOLA mRNA. The formulation is a lyophilized powder for injection, with excipients including mannitol and phosphate buffer. This invention also discloses the application of this drug in the preparation of a treatment for gastric cancer, specifically advanced gastric cancer with high PAPOLA expression. The drug is administered intravenously at a dose of 8-12 mg / kg every 3 days. Its mechanism of action is to block PAPOLA protein translation, inhibit the G1 / S phase transition of gastric cancer cells, and induce apoptosis. In vitro and in vivo experiments have confirmed that this drug can specifically inhibit the proliferation and growth of gastric cancer cells, providing a new treatment option for advanced gastric cancer.

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

The application relates to the technical field of biological medicine, and discloses application of a reagent for detecting CBS gene expression level in preparation of a breast cancer diagnosis and / or prognosis product. The nucleotide sequence of the CBS gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded CBS protein is shown as SEQ ID NO. 2. The experimental results of the application show that the expression of the CBS protein in breast cancer tissues is significantly higher than that in paracancerous tissues, and the CBS protein can be used as a biomarker for breast cancer diagnosis. Meanwhile, the ROC curve evaluation of the CBS protein expression amount on the prognosis results of breast cancer patients shows that the AUC value reaches 0.841, which also shows that the CBS protein can be used as a marker for the prognosis of breast cancer patients, and the CBS protein has important diagnostic significance for the prognosis of breast cancer.

This invention, entitled "An Optimized RNA Stabilizing Element and Its Application in Enhancing Transient Expression in Tobacco," belongs to the field of plant genetic engineering technology. The technical problem to be solved is to provide an optimized RNA stabilizing element capable of enhancing transient expression in tobacco and its application in this process. The key technical solution is to provide a method for enhancing the transient expression level of tobacco, comprising: introducing an optimized RNA stabilizing element into the expression vector of the target gene, wherein the optimized RNA stabilizing element is a 5′UTR core motif containing the nucleotide sequence shown in SEQ ID NO:1. This method effectively overcomes the bottleneck of exogenous gene expression in plants, providing a highly efficient expression enhancement tool for plant bioreactors and molecular breeding.

This invention relates to molecular marker 1769 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 1769 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals who have undergone sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The application discloses an Aspergillus specific gene and a detection method and application thereof, and belongs to the technical field of biotechnology and medical molecular diagnosis. The nucleotide sequence comprises a full length or a partial continuous fragment selected from any one of sequences shown in SEQ ID NO. 1 to SEQ ID NO. 20, or a complementary sequence thereof. In the detection, nucleic acid is extracted from a sample to be detected; a primer pair is used to perform a nucleic acid amplification reaction on the extracted nucleic acid; the presence or quantity of an amplification product is detected, and a real-time Ct value is analyzed; a critical value is determined based on a test on a known negative sample library; and whether Aspergillus is detected is determined according to the Ct value and the critical value. The application shows high specificity, sensitivity and repeatability in clinical sample detection, and the detection period can be shortened to within 4 hours, thereby solving problems of long detection period, low sensitivity and cross reaction of existing Aspergillus detection methods.

Provided is a recombinant adeno-associated viral (rAAV) vector comprising one or two of (a) to (c): (a) a nucleotide sequence encoding aromatic L-amino acid decarboxylase (AADC), (b) a nucleotide sequence encoding glucocerebrosidase (GBA1); and (c) a nucleotide sequence encoding a neurotrophic factor (NTF), such as cerebral dopamine neurotrophic factor (CDNF) or glial cell derived neurotrophic factor (GDNF), for treating neurodegenerative disorders, particularly Parkinson's disease (PD), Multiple system atrophy (MSA), Gaucher's disease (GD), and other proteinopathies. Also provided herein are viral particles comprising the rAAV vector, a pharmaceutical composition comprising the viral particles, and uses thereof.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The application discloses a nuclear factor YB subunit gene GhNF-YB3 and application thereof, the nucleotide sequence of the gene GhNF-YB3 is shown as SEQ ID NO. 1; through yield character investigation on 245 upland cotton varieties, GWAS and eQTL positioning are carried out in combination with population genome resequencing and ovule transcriptome sequencing one day after flowering, and a site gene GhNF-YB3 significantly related to cotton yield character is obtained. The gene expression of the gene GhNF-YB3 is significantly positively correlated with two yield characters of seed index and boll weight, and the gene may be a causal gene for regulating cotton seed index character. The nuclear factor YB subunit gene GhNF-YB3 of the application can be applied to identification of high-yield upland cotton varieties and improvement of cotton yield character.

The present disclosure provides virus-like particles (VLPs) comprising: i) a CRISPR-Cas effector polypeptide; ii) a recombinant lentivirus comprising a nucleotide sequence encoding a therapeutic polypeptide having a length of from about 250 amino acids to about 3,000 amino acids, where the VLP comprises a pseudotyping viral glycoprotein and / or a polypeptide that provides for binding to a target cell. The present disclosure provides systems for producing a VLP. The present disclosure provides methods of delivering a therapeutic protein, using a VLP of the present disclosure.

This invention discloses an immune cell that secretes type 2 cytokines under hypoxic conditions and its applications, belonging to the fields of genetic engineering and cell engineering. The invention first constructs a nucleotide fragment encoding hypoxia-induced type 2 cytokines, including a signal peptide gene sequence, a CAR molecule nucleotide sequence targeting CD19 and CD22, and an IL-4 or IL-10 nucleotide sequence containing multiple HRE motifs of a hypoxia-inducible promoter. This nucleotide fragment is delivered to immune cells for stable expression, yielding an immune cell that secretes type 2 cytokines under hypoxic conditions. This immune cell secretes IL-4 or IL-10 only under hypoxic conditions, thereby leveraging the metabolic reprogramming effect of IL-4 or IL-10 on immune cells to enhance their activity under hypoxic conditions, thus achieving better long-term anti-tumor effects.