55 results about "Smut" patented technology

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention discloses application of an OsMYB23 gene in improving resistance of rice to ustilaginoidea virens, and belongs to the technical field of gene engineering. The nucleotide sequence of the CDS region of the OsMYB23 gene is as shown in SEQ ID NO. 2. Researches find that the OsMYB23 gene is related to the resistance of rice to the false smut, the over-expression of the OsMYB23 gene significantly reduces the disease resistance of the rice to the false smut, the mutation of the OsMYB23 gene improves the defense capability of the rice to the false smut, and the mutation of the OsMYB23 gene does not influence the normal growth of the rice, so that the OsMYB23 gene of the rice can be used for improving the resistance of the rice to the false smut, and the rice can be used for preparing the rice for preventing and treating the false smut. And a theoretical basis is provided for prevention and control of rice ustilaginoidea virens and diseases caused by the rice

The invention discloses a promoter of a corn disease resistance related gene, a molecular marker and application of the promoter and the molecular marker. The invention provides the promoter of the TPS6 gene for regulating and controlling the resistance of the northern leaf blight, the southern leaf blight, the smut disease and the like of the corn as shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3, and the mutant of the promoter, which can regulate and control the expression of the TPS6 gene in the corn, and can be used for improving the resistance of the corn to the northern leaf blight, the southern leaf blight, the smut disease and the like or cultivating a new variety of disease-resistant corn. The invention provides a molecular marker Indel8 as a molecular marker for expression of a TPS6 gene in corn, and application of the molecular marker Indel8 in regulation of expression of TPS6 in corn and improvement of corn disease resistance.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses a method for creating a corn ear rot resistant germplasm and application of the corn ear rot resistant germplasm. The invention relates to the technical field of biology, and provides an application of inhibiting expression and / or activity of encoding protein of a Zm00001d023282 gene in any one of the following applications: (A1) improving the resistance of corn to spike rot; (A2) the resistance of corn to pathogenic bacteria of spike rot is improved; and (A3) the content of lignin in corn plants is increased. According to the invention, the Zm00001d023282 gene in a corn genome is knocked out through a gene editing means, so that the lignin content and the spike rot resistance of corn can be remarkably improved. The invention has important significance for quickly creating the corn ear rot resistant germplasm.

The invention provides bacillus subtilis and application thereof, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29256. The bacterial strain has a good broad-spectrum antibacterial effect, and can be used for preparing the bacillus subtilis. The microbial agent has good inhibition effects on various plant fungal pathogens and bacterial pathogens causing plant diseases, especially has good prevention and treatment effects on highland barley smut, the prevention and treatment effects on the highland barley smut can reach 95% or above by using 10,000,000 cfu / mL of a microbial agent diluent, and the microbial agent has excellent effects.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention discloses a rice bakanae disease bacterium-carrying seed detection method and device based on a hyperspectral image, and the method comprises the steps: preprocessing the hyperspectral data of rice seeds, and inputting the preprocessed data into a rice bakanae disease bacterium-carrying seed discrimination model; a hierarchical and adaptive feature learning and decision-making system is realized by using a multi-scale spectral feature extraction module, an adaptive spectral attention mechanism module, a deep feature cross fusion network module and a result discrimination module, the self-adaptive spectrum attention mechanism module dynamically analyzes the importance of each wave band, and the judgment result module adopts a parallel dynamic meta-learning classifier module and an uncertainty quantification module, and provides the credibility of a judgment result while outputting the judgment result of the seed health state. According to the method, efficient, accurate and lossless bacteria-carrying seed identification with quantifiable result reliability is realized.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention discloses a molecular marker and application thereof in identification of sorghum head smut resistance. The molecular marker is as shown in SEQ ID NO: 1. Experiments prove that when the nucleotide at the 217th site from the 5'terminal in the molecular marker is C, the sorghum has head smut resistance; when the nucleotide at the 217th site from the 5'terminal in the molecular marker is G, the sorghum does not have the head smut resistance, so that the molecular marker shown in the SEQ ID NO: 1 can identify the head smut resistance of the sorghum, and the molecular marker has important application value in sorghum breeding.

The invention discloses a method for efficiently breeding by using wild germplasm, and relates to the technical field of agricultural breeding, the method comprises the following steps: hybridizing Guangdong 93-159 as a female parent and GXAS07-6-1 containing saccharum arundinaceum and hemsleya amabilis consanguinity as a male parent to obtain a hybrid A; using cinnamyl sugar 05-164 as a female parent to hybridize with the hybrid A to obtain a hybrid B; hybridizing Guitan 29 serving as a female parent with the hybrid seed B to obtain a hybrid seed C; according to the method, the low-fertility sugarcane variety with poor pollen scattering, low pollen development rate and high sugar content and the high-fertility wild sugarcane with adaptability, disease resistance, cold resistance, high drought resistance and low sugar content are selected as parents for cross breeding, and the high fertility and high resistance of the wild sugarcane make up for the defect of insufficient breeding capacity of the low-fertility sugarcane variety; the breeding efficiency is improved by using wild germplasm, and the obtained hybrid variety is high in sugar, has many effective stems, good growth vigor, strong smut resistance and obvious super-parent advantage.

The invention discloses a marker closely linked with corn smut resistance and application of the marker, and belongs to the technical field of molecular marker assisted breeding. According to the invention, two markers, namely qSB1 and qSB5, which are closely linked with the corn anti-tumor smut disease are found for the first time, and the qSB1 is located at the 24428166 site of the No.1 chromosome; qSB5 is located at the site 220156746 of the chromosome 5; when the basic groups marked as qSB1 and qSB5 are T and C respectively, the corn shows high resistance to the corn smut disease; the invention further provides a KASP primer for detecting two markers which are closely linked with the corn anti-tumor smut disease, and the sequence of the KASP primer of the qSB1 marker is as shown in SEQ ID NO.3-SEQ ID NO.5; a KASP primer sequence marked by the qSB5 is as shown in SEQ ID NO. 6 to SEQ ID NO. 8; the provided marker closely linked with the corn anti-tumor smut disease and the related KASP primer for detecting the marker can be used for breeding the corn anti-tumor smut disease variety and identifying the anti-tumor smut disease performance, and have important significance for saving the breeding cost, improving the breeding efficiency and shortening the breeding period.

The application discloses a molecular marker for identifying the resistance of wheat to black smut and a method thereof. The application provides application of a single nucleotide polymorphism of a specific SNP site (located at 37 of SEQ ID No. 4 on a 6A chromosome of wheat, T or C) on a wheat genome as a marker in identifying or assisting in identifying the resistance of wheat to black smut. The application develops a KASP primer according to the specific SNP site, and molecular marker assisted screening can be performed on a black smut gene. The application has important significance for cultivating a wheat variety with enhanced resistance to black smut.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention belongs to the technical field of agricultural disease control, and discloses a fusarium pseudograminearum GPI anchoring protein and an application of a coding gene of the fusarium pseudograminearum GPI anchoring protein. The fusarium pseudograminearum FpPer1 gene is knocked out, so that the mycelial growth rate, conidium yield and pathogenicity of the fusarium pseudograminearum FpPer1 gene are remarkably inhibited, and meanwhile, the gene knockout strain is more sensitive to bactericides such as tebuconazole. The gene can be used as a novel target for designing a pesticide synergist; or a small molecule compound, polypeptide or nucleic acid medicine is designed based on the FpPer1 gene or the FpPer1 protein coded by the FpPer1 gene, and is compounded with the existing bactericide (such as tebuconazole) to improve the pesticide effect. A new target is provided for prevention and control of wheat stem rot, efficient prevention and control are achieved by interfering a pathogen protein post-translational modification approach, the toxin pollution risk is reduced, and wide agricultural application prospects are achieved.

The application discloses a SNP molecular marker related to corn head smut resistance and application thereof. The molecular marker is DNdCAPs837, which is located at the 837th site of a corn chromosome 4 ZmMYB42 Gene, and the mutation base is G / C. Using corn genome total DNA as a template, a dCAPS primer pair is used for PCR amplification, the amplification product is 224bp, the 27th base is a mutation site, the site of a susceptible material is C, and the site of a resistant material is G, which can be cut into two fragments of 197bp and 27bp by KpnI restriction endonuclease. Based on the detection result of the molecular marker, the head smut resistance of a plant can be identified. The molecular marker provided by the application can be used for breeding of a disease-resistant plant, and provides a new technical means and method basis for screening and creating a new material resistant to head smut, and has important value in the field of plant breeding.

The application relates to the technical field of genetic engineering, and discloses application of a transcription factor TabHLH168 protein in wheat disease resistance. The amino acid sequence of the TabHLH168 protein is shown as SEQ ID NO. 1; the coding gene sequence of the TabHLH168 protein is shown as SEQ ID NO. 2. The TabHLH168 protein is a target protein of a false smut fungus effect protein FpE02. The application uses the above-mentioned application of the transcription factor TabHLH168 protein in wheat disease resistance, overexpresses the coding gene of the TabHLH168 protein, obtains transgenic wheat with stable heredity and wheat stem base rot resistance, provides a new solution for carrying out wheat disease resistance breeding work, and has important significance in the field of wheat breeding with disease resistance.

The invention discloses an SNP molecular marker related to corn head smut resistance and application of the SNP molecular marker. The molecular marker is DNdCAPs837 and is located at the 837th site of a ZmMYB42 gene of a No.4 chromosome of corn, and a mutant basic group is G / C. The corn genome total DNA is used as a template, a dCAPS primer pair is used for PCR amplification, the size of an amplification product is 224bp, the 27th basic group is a mutation site, the site of a susceptible material is C and cannot be cut by KpnI restriction endonuclease, and the site of a disease-resistant material is G and can be cut into two fragments of 197bp and 27bp by KpnI restriction endonuclease. The head smut resistance identification of plants can be realized based on the detection result of the molecular marker. The molecular marker provided by the invention can be used for cultivating disease-resistant plants, provides a new technical means and method basis for screening and creating a head smut-resistant new material, and has important value in the field of plant breeding.

The invention discloses a microorganism-based nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of plant disease control. In order to solve the technical problems of environmental pollution, soil deterioration and bacterial drug resistance enhancement in a chemical agronomy prevention and treatment method for tobacco bacterial wilt in the prior art, the invention provides a microorganism-based nano-enzyme, and the microorganism-based nano-enzyme is prepared by carrying out high-temperature carbonization treatment on smut teliospore pretreated by low-temperature plasma, and by taking the generated biomass carbon particles as a template, carrying out manganese ion deposition treatment to obtain the manganese-based nano-enzyme. According to the microorganism-based nano-enzyme provided by the invention, the use of chemical pesticides is reduced, and the microorganism-based nano-enzyme is relatively friendly to the environment, free of secondary pollution and relatively high in safety; the provided microorganism-based nano-enzyme improves the prevention and treatment effect of ralstonia solanacearum, and a new way is provided for application in plant disease prevention and treatment.

The invention discloses application of a gene TaPT31-7A in the aspect of improving pre-harvest sprouting resistance, and relates to the technical field of agricultural biologication.TaPT31-7A is determined to serve as a phosphorus transporter gene, and a homozygous plant over-expressing the TaPT31-7A gene remarkably improves the wheat pre-harvest sprouting resistance in four environments in two years, so that the TaPT31-7A gene has the function of regulating and controlling the pre-harvest sprouting resistance, and the wheat pre-harvest sprouting resistance is improved. The method can be used for improving wheat pre-harvest sprouting resistance and accelerating the breeding process of pre-harvest sprouting resistant wheat, and important technical support is provided for cultivating high-resistance wheat varieties.

The application discloses use of an extract of black tiger in preparation of a fungicide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The black tiger extract has the effects of inhibiting spore germination, germ tube growth or zoospore formation, and vegetative hypha growth of the fungi and oomycetes, can effectively prevent growth of the pathogenic fungi and infection on host plants, and has certain prevention and treatment effects on rice rice blast, rice rice smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper blight and camphor blight, and provides a possibility for development of a plant source fungicide with diversified effective components, high prevention and treatment efficiency and a wide spectrum of prevention and treatment objects.

This invention discloses a compound seed treatment agent containing metalaxyl-M and tebuconazole, its preparation method, and its uses, belonging to the technical field of seed treatment agents. The suspension of this invention uses metalaxyl-M, tebuconazole, and sodium selenite as the main components, supplemented with emulsifying and dispersing agents, wetting and dispersing agents, antifreeze agents, suspending agents, film-forming agents, and warning colors, and is prepared by adding deionized water to a sufficient volume. Metalaxyl-M targets and inhibits ribosomal RNA polymerase I of oomycete pathogens, while tebuconazole targets and inhibits sterol 14α-demethylase. The two have complementary fungicidal spectra, synergistically controlling maize stalk rot and maize head smut. Sodium selenite, by inhibiting the oxidative stress defense system of pathogenic fungi involving glutathione and thioredoxin, promotes the effects of the two main components and simultaneously remediates selenium-deficient soils. This invention is suitable for the green and efficient control of maize stalk rot and head smut, and also has the function of ecological restoration of selenium-deficient soils.

The application discloses a kind of compound medicament and wheat stem base rot comprehensive prevention method, belong to plant disease control technical field.The application is prepared by using compound medicament and seed coating agent by combining streptomyces albus S86 fermentation liquor with kiralate medicament, it is found that the combination has obvious synergistic effect, the inhibition rate of false smut fungus spore germination is higher than single-agent medicament, it is 72.65%;Wheat stem base rot has better field control effect, disease index is 0.02, and the control effect is 73.85%.Field intuition shows that the disease degree of streptomyces albus S86 fermentation liquor and kiralate medicament combination treatment group is lighter than other treatment groups as a whole.This provides a basis for the biological control of wheat stem base rot in the future, and has wide application prospect.

The application discloses a wheat rhizosphere growth promoting composite microbial agent and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms.The wheat rhizosphere growth promoting composite microbial agent has the functions of nitrogen fixation, phosphorus dissolution and IAA production, and is beneficial to nutrient utilization of wheat; after wheat seedlings are connected with the wheat rhizosphere growth promoting composite microbial agent, the nitrogen absorption efficiency can be significantly improved, and the growth of the wheat is promoted, so that the purpose of reducing fertilizer and increasing efficiency is achieved; moreover, the wheat rhizosphere growth promoting composite microbial agent has an antagonistic effect on multiple pathogenic fungi such as false smut fungus, fusarium graminearum, fusarium solani and the like, and can be used for biological control of wheat.

The application discloses an application of a rice smut pathogenicity factor ThSCSP_6 in improving plant disease resistance, and belongs to the technical field of genetic engineering. The amino acid sequence of the rice smut pathogenicity factor ThSCSP_6 is shown as SEQ ID NO:1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO:2. Through cloning and function analysis of the rice smut pathogenicity factor ThSCSP_6, the application helps to reveal the molecular mechanism of specific interaction between a rice smut pathogenicity factor race and a rice variety and evolution, and further effectively controls the occurrence of rice smut.

The invention provides a microecological preparation for preventing and treating oat diseases and insect pests and promoting growth as well as a preparation method and application of the microecological preparation, and belongs to the technical field of plant disease prevention and treatment. The microecological preparation comprises a probiotic fermentation composition and auxiliary materials, wherein the probiotic components in the probiotic fermentation composition comprise bacillus velezensis, pseudomonas aeruginosa, coxakella cocori and bacillus atrophaeus. According to the invention, four strains which can promote growth and have an antagonistic effect on plant disease bacteria are separated and screened from 500 high-quality oats. After being compounded into a microecological preparation for seed dressing, the microecological preparation can effectively inhibit infection of pathogenic bacteria on the surfaces of seeds, the growth state of the microecological preparation is indifferent from that of normally planted oat, the morbidity of smut, powdery mildew and leaf spot caused by endohilum helminthosporium can be effectively reduced, and the cure rate of the microecological preparation is superior to that of sterilization.

The invention discloses a method for detecting Tilletia foetida spores by combining ICP-MS with nanogold, and belongs to the technical field of biological detection.The method comprises the steps that a biotin solution reacts with a Tilletia foetida spore polyclonal antibody to obtain a biotin-labeled polyclonal antibody, streptavidin magnetic beads are added, incubation is conducted to obtain immunomagnetic beads, chloroauric acid is added into deionized water, and the immunomagnetic beads are added into the deionized water; the method comprises the following steps: adding sodium citrate after boiling to obtain a nano-gold solution, uniformly mixing the nano-gold solution with a Tilletia foetida spore monoclonal antibody, adding BSA, centrifuging to obtain a nano-gold probe, closing immunomagnetic beads, adding a sample solution containing Tilletia foetida spores, and performing magnetic separation to obtain a spore-magnetic bead compound; the method comprises the following steps: sealing a nanogold probe with a skim milk powder solution, adding a spore-magnetic bead compound, performing magnetic separation to obtain a magnetic bead-spore-nanogold compound, adding a citric acid solution into the magnetic bead-spore-nanogold compound, performing desorption to obtain a desorption solution, and introducing the desorption solution into ICP-MS for detection, so that the sensitivity is high, and the detection is rapid.

The invention discloses a rice kernel smut fungus effector ThSCSP20 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the effector ThSCSP20 is as shown in SEQ ID NO: 1. Through cloning of the rice kernel smut fungus effect factor ThSCSP20 and function analysis of the rice kernel smut fungus effect factor ThSCSP20 in plant immunity excitation, the specific interaction between the rice kernel smut fungus microspecies and the rice variety and the molecular mechanism of evolution of the specific interaction are disclosed, and a basis is provided for further exploration of the interaction mechanism of the rice kernel smut fungus and a host.