37 results about "Smut" patented technology

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention discloses application of an OsMYB23 gene in improving resistance of rice to ustilaginoidea virens, and belongs to the technical field of gene engineering. The nucleotide sequence of the CDS region of the OsMYB23 gene is as shown in SEQ ID NO. 2. Researches find that the OsMYB23 gene is related to the resistance of rice to the false smut, the over-expression of the OsMYB23 gene significantly reduces the disease resistance of the rice to the false smut, the mutation of the OsMYB23 gene improves the defense capability of the rice to the false smut, and the mutation of the OsMYB23 gene does not influence the normal growth of the rice, so that the OsMYB23 gene of the rice can be used for improving the resistance of the rice to the false smut, and the rice can be used for preparing the rice for preventing and treating the false smut. And a theoretical basis is provided for prevention and control of rice ustilaginoidea virens and diseases caused by the rice

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses a method for creating a corn ear rot resistant germplasm and application of the corn ear rot resistant germplasm. The invention relates to the technical field of biology, and provides an application of inhibiting expression and / or activity of encoding protein of a Zm00001d023282 gene in any one of the following applications: (A1) improving the resistance of corn to spike rot; (A2) the resistance of corn to pathogenic bacteria of spike rot is improved; and (A3) the content of lignin in corn plants is increased. According to the invention, the Zm00001d023282 gene in a corn genome is knocked out through a gene editing means, so that the lignin content and the spike rot resistance of corn can be remarkably improved. The invention has important significance for quickly creating the corn ear rot resistant germplasm.

The invention provides bacillus subtilis and application thereof, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29256. The bacterial strain has a good broad-spectrum antibacterial effect, and can be used for preparing the bacillus subtilis. The microbial agent has good inhibition effects on various plant fungal pathogens and bacterial pathogens causing plant diseases, especially has good prevention and treatment effects on highland barley smut, the prevention and treatment effects on the highland barley smut can reach 95% or above by using 10,000,000 cfu / mL of a microbial agent diluent, and the microbial agent has excellent effects.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention discloses a rice bakanae disease bacterium-carrying seed detection method and device based on a hyperspectral image, and the method comprises the steps: preprocessing the hyperspectral data of rice seeds, and inputting the preprocessed data into a rice bakanae disease bacterium-carrying seed discrimination model; a hierarchical and adaptive feature learning and decision-making system is realized by using a multi-scale spectral feature extraction module, an adaptive spectral attention mechanism module, a deep feature cross fusion network module and a result discrimination module, the self-adaptive spectrum attention mechanism module dynamically analyzes the importance of each wave band, and the judgment result module adopts a parallel dynamic meta-learning classifier module and an uncertainty quantification module, and provides the credibility of a judgment result while outputting the judgment result of the seed health state. According to the method, efficient, accurate and lossless bacteria-carrying seed identification with quantifiable result reliability is realized.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention belongs to the technical field of agricultural disease control, and discloses a fusarium pseudograminearum GPI anchoring protein and an application of a coding gene of the fusarium pseudograminearum GPI anchoring protein. The fusarium pseudograminearum FpPer1 gene is knocked out, so that the mycelial growth rate, conidium yield and pathogenicity of the fusarium pseudograminearum FpPer1 gene are remarkably inhibited, and meanwhile, the gene knockout strain is more sensitive to bactericides such as tebuconazole. The gene can be used as a novel target for designing a pesticide synergist; or a small molecule compound, polypeptide or nucleic acid medicine is designed based on the FpPer1 gene or the FpPer1 protein coded by the FpPer1 gene, and is compounded with the existing bactericide (such as tebuconazole) to improve the pesticide effect. A new target is provided for prevention and control of wheat stem rot, efficient prevention and control are achieved by interfering a pathogen protein post-translational modification approach, the toxin pollution risk is reduced, and wide agricultural application prospects are achieved.

The application relates to the technical field of genetic engineering, and discloses application of a transcription factor TabHLH168 protein in wheat disease resistance. The amino acid sequence of the TabHLH168 protein is shown as SEQ ID NO. 1; the coding gene sequence of the TabHLH168 protein is shown as SEQ ID NO. 2. The TabHLH168 protein is a target protein of a false smut fungus effect protein FpE02. The application uses the above-mentioned application of the transcription factor TabHLH168 protein in wheat disease resistance, overexpresses the coding gene of the TabHLH168 protein, obtains transgenic wheat with stable heredity and wheat stem base rot resistance, provides a new solution for carrying out wheat disease resistance breeding work, and has important significance in the field of wheat breeding with disease resistance.

The invention discloses application of a gene TaPT31-7A in the aspect of improving pre-harvest sprouting resistance, and relates to the technical field of agricultural biologication.TaPT31-7A is determined to serve as a phosphorus transporter gene, and a homozygous plant over-expressing the TaPT31-7A gene remarkably improves the wheat pre-harvest sprouting resistance in four environments in two years, so that the TaPT31-7A gene has the function of regulating and controlling the pre-harvest sprouting resistance, and the wheat pre-harvest sprouting resistance is improved. The method can be used for improving wheat pre-harvest sprouting resistance and accelerating the breeding process of pre-harvest sprouting resistant wheat, and important technical support is provided for cultivating high-resistance wheat varieties.

The application discloses use of an extract of black tiger in preparation of a fungicide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The black tiger extract has the effects of inhibiting spore germination, germ tube growth or zoospore formation, and vegetative hypha growth of the fungi and oomycetes, can effectively prevent growth of the pathogenic fungi and infection on host plants, and has certain prevention and treatment effects on rice rice blast, rice rice smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper blight and camphor blight, and provides a possibility for development of a plant source fungicide with diversified effective components, high prevention and treatment efficiency and a wide spectrum of prevention and treatment objects.

This invention discloses a compound seed treatment agent containing metalaxyl-M and tebuconazole, its preparation method, and its uses, belonging to the technical field of seed treatment agents. The suspension of this invention uses metalaxyl-M, tebuconazole, and sodium selenite as the main components, supplemented with emulsifying and dispersing agents, wetting and dispersing agents, antifreeze agents, suspending agents, film-forming agents, and warning colors, and is prepared by adding deionized water to a sufficient volume. Metalaxyl-M targets and inhibits ribosomal RNA polymerase I of oomycete pathogens, while tebuconazole targets and inhibits sterol 14α-demethylase. The two have complementary fungicidal spectra, synergistically controlling maize stalk rot and maize head smut. Sodium selenite, by inhibiting the oxidative stress defense system of pathogenic fungi involving glutathione and thioredoxin, promotes the effects of the two main components and simultaneously remediates selenium-deficient soils. This invention is suitable for the green and efficient control of maize stalk rot and head smut, and also has the function of ecological restoration of selenium-deficient soils.

The application discloses a kind of compound medicament and wheat stem base rot comprehensive prevention method, belong to plant disease control technical field.The application is prepared by using compound medicament and seed coating agent by combining streptomyces albus S86 fermentation liquor with kiralate medicament, it is found that the combination has obvious synergistic effect, the inhibition rate of false smut fungus spore germination is higher than single-agent medicament, it is 72.65%;Wheat stem base rot has better field control effect, disease index is 0.02, and the control effect is 73.85%.Field intuition shows that the disease degree of streptomyces albus S86 fermentation liquor and kiralate medicament combination treatment group is lighter than other treatment groups as a whole.This provides a basis for the biological control of wheat stem base rot in the future, and has wide application prospect.

The application discloses a wheat rhizosphere growth promoting composite microbial agent and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms.The wheat rhizosphere growth promoting composite microbial agent has the functions of nitrogen fixation, phosphorus dissolution and IAA production, and is beneficial to nutrient utilization of wheat; after wheat seedlings are connected with the wheat rhizosphere growth promoting composite microbial agent, the nitrogen absorption efficiency can be significantly improved, and the growth of the wheat is promoted, so that the purpose of reducing fertilizer and increasing efficiency is achieved; moreover, the wheat rhizosphere growth promoting composite microbial agent has an antagonistic effect on multiple pathogenic fungi such as false smut fungus, fusarium graminearum, fusarium solani and the like, and can be used for biological control of wheat.

The application discloses an application of a rice smut pathogenicity factor ThSCSP_6 in improving plant disease resistance, and belongs to the technical field of genetic engineering. The amino acid sequence of the rice smut pathogenicity factor ThSCSP_6 is shown as SEQ ID NO:1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO:2. Through cloning and function analysis of the rice smut pathogenicity factor ThSCSP_6, the application helps to reveal the molecular mechanism of specific interaction between a rice smut pathogenicity factor race and a rice variety and evolution, and further effectively controls the occurrence of rice smut.

The invention provides a microecological preparation for preventing and treating oat diseases and insect pests and promoting growth as well as a preparation method and application of the microecological preparation, and belongs to the technical field of plant disease prevention and treatment. The microecological preparation comprises a probiotic fermentation composition and auxiliary materials, wherein the probiotic components in the probiotic fermentation composition comprise bacillus velezensis, pseudomonas aeruginosa, coxakella cocori and bacillus atrophaeus. According to the invention, four strains which can promote growth and have an antagonistic effect on plant disease bacteria are separated and screened from 500 high-quality oats. After being compounded into a microecological preparation for seed dressing, the microecological preparation can effectively inhibit infection of pathogenic bacteria on the surfaces of seeds, the growth state of the microecological preparation is indifferent from that of normally planted oat, the morbidity of smut, powdery mildew and leaf spot caused by endohilum helminthosporium can be effectively reduced, and the cure rate of the microecological preparation is superior to that of sterilization.

The invention discloses a rice kernel smut fungus effector ThSCSP20 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the effector ThSCSP20 is as shown in SEQ ID NO: 1. Through cloning of the rice kernel smut fungus effect factor ThSCSP20 and function analysis of the rice kernel smut fungus effect factor ThSCSP20 in plant immunity excitation, the specific interaction between the rice kernel smut fungus microspecies and the rice variety and the molecular mechanism of evolution of the specific interaction are disclosed, and a basis is provided for further exploration of the interaction mechanism of the rice kernel smut fungus and a host.

The invention provides an identification method of wheat basal stem rot resistance, and belongs to the technical field of disease identification. According to the method for identifying the wheat basal stem rot resistance, the specific numerical value of the fusarium pseudograminearum biomass is used as a criterion, the fusarium pseudograminearum biomass in stalks and the wheat variety resistance grade are in extremely significant positive correlation, a linear equation is established, subjective errors of traditional manual investigation and interference of environmental heterogeneity are completely avoided, and the method has the advantages that the method is simple and convenient to operate, and the identification accuracy is high. The identification result can be quantified and repeatable, and the inherent resistance of the variety is truly reflected. The method aims at solving the problem that although the browning degree of stalks of part of wheat varieties is not deep, hyphae grow in the stalks, and the wheat basal stem rot resistance cannot be judged only according to the apparent browning degree. In addition, when resistance evaluation is carried out on some precious and rare breeding materials, the breeding materials can be saved and preserved. Therefore, by detecting the biomass of the fusarium pseudograminearum, the resistance level of the wheat basal stem rot can be reliably judged, and prospective guidance is provided for breeding of disease-resistant breeding.

The present application is suitable for the technical field of sorghum fusarium head blight identification, and provides a molecular marker for identifying sorghum resistance or susceptibility to fusarium head blight, comprising a molecular marker 6Sam72772 and / or Tx430508; the molecular marker 6Sam72772 can be a 126bp DNA fragment and a 122bp DNA fragment, the nucleotide sequence of the 126bp DNA fragment is shown as SEQ ID:5, and the nucleotide sequence of the 122bp DNA fragment is shown as SEQ ID:6; the molecular marker Tx430508 is a 720bp DNA fragment, and the nucleotide sequence is shown as SEQ ID:7. The present application also provides related primer groups, kits, and methods for identifying sorghum resistance or susceptibility to fusarium head blight. The present application provides the molecular markers 6Sam72772 and Tx430508, which can accurately identify or assist in identifying sorghum resistance or susceptibility to fusarium head blight.

The application belongs to the field of biological detection, and particularly relates to a RPA-LFD triple detection method and kit for rice blast, rice smut and sheath blight bacteria and application thereof. The detection method comprises the following steps: S.1 extraction of strain and sample DNA; S.2 design of primer and probe: corresponding RPA primers and probes are designed according to specific target sequences of rice blast, rice smut and sheath blight bacteria genomic DNA; S.3 sensitivity determination of multiplex RPA-LFD reaction system. The application can simultaneously complete synchronous detection of three kinds of pathogens in a single tube by designing multiple specific primers and probes through multiplex RPA, thereby improving detection efficiency; the detection throughput of multiplex RPA-LFD technology is improved by more than 3 times, and reagent consumption is reduced by 60%.

The invention discloses a rice kernel smut effector factor ThSCSP35 and application thereof, and belongs to the technical field of genetic engineering. The effector factor ThSCSP35 is separated and cloned from the rice kernel smut, and the nucleotide sequence of the effector factor ThSCSP35 is shown as SEQ ID NO: 1. Through cloning and functional analysis of the rice kernel smut fungus effector factor ThSCSP35, the molecular mechanism of specific interaction and evolution between the rice kernel smut fungus microspecies and the rice variety is disclosed, so that the occurrence of the rice kernel smut fungus is effectively controlled.

The application relates to the sugarcane planting technical field and discloses a sugarcane stalk inoculation with smut fungus automatic inoculation device and method.The sugarcane stalk inoculation with smut fungus automatic inoculation device comprises a base, a plurality of support frames fixedly arranged above the base, a pay-off roller and a winding roller rotatably arranged on the inner side of the support frame, a strip-shaped adhesive tape, a cutting assembly arranged between the pay-off roller and the winding roller and a water-retaining cotton supply assembly; the water-retaining cotton supply assembly, the spray head and the rectangular adhesive tape precise fitting structure are matched and arranged, the traditional whole soaking inoculation mode of the sugarcane stalk is abandoned, the spore liquid is accurately supplied and targeted inoculation operation is realized; the water-retaining cotton is adsorbed with spore liquid through the high-density hydrophilic non-woven fabric material, and the water-retaining cotton is accurately coated on the sugarcane bud inoculation target point by relying on the PET waterproof rectangular adhesive tape; only the sugarcane bud part is inoculated with the pathogenic bacteria, a large-capacity spore suspension is not needed, and the use amount of the smut spore powder and the solvent is greatly reduced.

The present application relates to the technical field of agricultural biological control, and particularly relates to a streptomyces spinogriseus LZ-337 and application thereof. Streptomyces clavifer The classification name of the streptomyces spinogriseus LZ-337 is The preservation number is CGMCC No. 36504, the preservation center is Chinese General Microbiological Culture Collection Center, the preservation date is November 5, 2025, and the preservation address is No. 1, Yihuangyuan 3rd, Beichen West Road, Chaoyang District, Beijing, China. The streptomyces spinogriseus LZ-337 can be used for preventing and treating rice sheath blight, and also has certain antagonistic effect on rice blast fungus and rice smut fungus. The field test result shows that the fermentation liquor of the streptomyces spinogriseus LZ-337 has a prevention effect of 80.35% on rice sheath blight, effectively reduces the yield reduction of rice caused by diseases, and provides strong support for guaranteeing food security.

The present application relates to the field of agricultural microorganism technology, and particularly discloses a microbial inoculum for promoting growth in the prevention and treatment of rice smut, and a preparation method and application thereof. The present application provides an application of the grass Helicobacter R3 in the preparation of a microbial inoculum for promoting growth in the prevention and treatment of rice smut, and the classification name of the grass Helicobacter R3 is Herbaspirillum sp. R3, which was preserved in the China Center for Type Culture Collection on September 20, 2023, at an address of Wuhan University, China, Wuhan, with a preservation number of CCTCC NO: M 20231757. The microbial inoculum prepared from the grass Helicobacter R3 bacterial suspension, xanthan gum and ascorbic acid in a specific ratio, in combination with a disinfectant, can be applied to the prevention and treatment of seed diseases of rice, can significantly reduce the bacterial carrying rate of the rice smut pathogen of rice seeds, and can also alleviate the oxidative damage caused by seed disinfection and improve the seed germination rate.

The application discloses a kind of rice seed detection method and device based on hyperspectral image of seed-borne smut, the hyperspectral data of rice seed is inputted after pretreatment rice seed-borne smut discrimination model, utilize multiscale spectral feature extraction module, adaptive spectral attention mechanism module, deep feature cross fusion network module, discrimination result module, realize hierarchical, adaptive feature learning and decision system, wherein, multiscale spectral feature extraction module realizes the extraction of different scale spectral features, adaptive spectral attention mechanism module dynamically analyzes the importance of each band, discrimination result module uses parallel dynamic meta-learning classifier module and uncertainty quantification module, while providing the credibility of the judgment result, the discrimination result of output seed health state is provided.The application realizes efficient, accurate, non-destructive, quantifiable result reliability of seed-borne smut identification.

This invention belongs to the field of molecular biology detection technology for plant fungi, and discloses a LAMP primer set, reagent kit, and detection method for detecting the pathogen of oat smut. Based on the pathogen of oat smut... Ustilago hordei A set of LAMP-specific primers was designed based on specific sequences from the whole genome. The results were determined using real-time quantitative PCR, agarose gel electrophoresis, and SYBR Green I fluorescent dye assay to detect the oat smut pathogen carried on oat seeds. The LAMP detection method for oat smut pathogen established in this invention is highly specific, sensitive, fast, and low-cost, providing a new technical means for detecting oat smut pathogen carried on oat seeds and possessing high practical application value.