8 results about "Cytochrome P450" patented technology

This invention relates to a cytochrome P450 oxidase STO1, its encoding gene, and its applications. This invention is the first to clone and identify a cytochrome P450 oxidase gene, STO1, from potato responsible for catalyzing the synthesis of spirogelothoraxone, and to determine the nucleotide sequence and translated amino acid sequence of this gene. This invention provides expression vectors, recombinant microorganisms, or transgenic cell lines containing this gene. This invention constructs an engineered Saccharomyces cerevisiae strain for producing spirogelothoraxone, in which the strong promoter ERG9 is replaced by the weak promoter HXT1 in the strain's genome, and the STO1, AtCPR, tHMG1, and STS genes are integrated. This invention also provides a method for constructing this engineered strain and a method for producing spirogelothoraxone using this engineered strain, which can be used for the preparation of spirogelothoraxone.

This invention discloses a herbicide-resistant cytochrome P450 gene, BjCYP71C2, its protein, plasmid, and their applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene is SEQ ID NO:1 or has at least 80% identity with SEQ ID NO:1. This invention also discloses the protein polypeptide and plasmid encoded by the above gene. The purpose of this gene is to express it transgenic in plants, cultivating herbicide-resistant plants for selective weed control using herbicides.

The application relates to the technical field of molecular markers, and discloses an SNP site associated with turbot albinism, a molecular marker and application, wherein the SNP mutation site is a base T / A mutation at the 50th bp of a coding region of a gene KIT proto-oncogene, receptor tyrosine kinase a (kita); the SNP mutation site is a base A / T mutation at the 137th bp of a coding region of a gene cytochrome P450 3A40-like (LOC118311907); the SNP mutation site is a base G / T mutation at the 371st bp of a coding region of a gene kit ligand a (kitlga); and the SNP mutation site is a base G / A mutation at the 581st bp of a coding region of a gene frizzled class receptor 10 (fzd10). The SNP site associated with turbot albinism, the molecular marker and the application provide a precise screening method for turbot breeding. In the breeding process, the SNP molecular markers are used to determine whether the genotype is albinism or normal, to establish an excellent family with normal body color, to reduce the incidence of abnormal body color, and to improve the overall quality and economic benefits of turbot culture.

The application belongs to the technical field of diagnostic markers, and particularly relates to a pathological diagnosis biomarker combination for adrenal Cushing syndrome and application. The pathological diagnosis biomarker combination comprises low density lipoprotein receptor (LDLR), 3-hydroxy-3-methylglutaryl coenzyme A synthetase 1 (HMGCS1) and cytochrome P450 11B1 (CYP11B1). The area under the ROC curve (AUC) of the pathological diagnosis biomarker combination can reach 0.881 (95% confidence interval (CI): 0.775-987; sensitivity: 85.7%; specificity: 85.0%), which indicates that the combination of LDLR, HMGCS1 and CYP11B1 as the pathological diagnosis biomarker combination for adrenal Cushing syndrome has high accuracy.

The present application relates to a cytochrome P450 oxidase, and a gene encoding the cytochrome P450 oxidase, the enzyme is involved in the final key step of the biosynthesis of important active ingredients of Tripterygium wilfordii, triptolide and / or neo-triptolide, and can be used for biosynthesis of triptolide and / or neo-triptolide.

This invention provides a cytochrome P450 gene HvCYP94C1 and its application in improving the resistance of highland barley to powdery mildew, belonging to the field of agricultural biotechnology. The cytochrome P450 gene HvCYP94C1 of this invention can hydroxylate JA and JA-Ile to 12-OH-JA-Ile and 12-COOH-JA-Ile, respectively. Silencing this gene in powdery mildew-sensitive materials can significantly increase the jasmonic acid content, thereby improving the disease resistance of the materials, showing excellent application prospects.

The present invention provides compounds for the regulation of P-glycoprotein and cytochrome P450 (e.g., CYP3A4 and CYP3A5 isoforms) enzymes, which a) significantly improve the bioavailability of substrates of these enzymes, including anticancer drugs, b) overcome multidrug resistance in tumors, and c) reduce serious side effects while improving the delivery of P-glycoprotein substrates to the brain. [Solution] Compounds of formula (I), as well as their prodrugs and pharmaceutically acceptable salts, are provided. Further disclosure relates to pharmaceutical compositions containing the compounds, methods of use, and methods for preparing them. TIFF2026086798000135.tif39128

A method for preparing an organosilicon compound having at least one silicon-bonded carbinol group and / or at least one silanol group, having a cytochrome P450 variant that promotes oxidation of a silicon-bonded hydrocarbyl group in the presence of an oxidizing agent. The method comprises: combining a cytochrome P450 variant, an initial organosilicon compound having at least one silicon-bonded hydrocarbyl group, and a cofactor to obtain a reaction mixture; and exposing the reaction mixture to an oxidizing agent to oxidize the silicon-bonded hydrocarbyl group of the initial organosilicon compound, thereby preparing an organosilicon compound having at least one silicon-bonded carbinol group and / or at least one silanol group. Cytochrome P450 variants suitable for use in this method are also disclosed, along with methods for manipulating and optimizing them. Nucleic acids and compositions encoding cytochrome P450 variants, expression vectors, and host cells containing them are also disclosed.