50 results about "Cytochrome P450" patented technology

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

This invention relates to a cytochrome P450 oxidase STO1, its encoding gene, and its applications. This invention is the first to clone and identify a cytochrome P450 oxidase gene, STO1, from potato responsible for catalyzing the synthesis of spirogelothoraxone, and to determine the nucleotide sequence and translated amino acid sequence of this gene. This invention provides expression vectors, recombinant microorganisms, or transgenic cell lines containing this gene. This invention constructs an engineered Saccharomyces cerevisiae strain for producing spirogelothoraxone, in which the strong promoter ERG9 is replaced by the weak promoter HXT1 in the strain's genome, and the STO1, AtCPR, tHMG1, and STS genes are integrated. This invention also provides a method for constructing this engineered strain and a method for producing spirogelothoraxone using this engineered strain, which can be used for the preparation of spirogelothoraxone.

Provided are: a method for measuring one or more biomarkers to simply and accurately diagnose diseases associated with changes in the activities of cytochrome P450 metabolic enzymes (hereinafter referred to as "P450s"); and a method for detecting the one or more biomarkers. The methods focus on luminescence-based detection of the enzymatic activities of P450s, and involves reacting one or more P450s present in a sample collected in a low- or non-invasive manner, such as serum or urine, with a precursor of a luminescent / light-emitting substrate, metabolizing a luminescent substrate converted from the precursor to generate luminescence, and measuring the generated luminescence. Since many molecular species of P450s are known, the activities of different molecular species of P450s can be measured for each disease on the basis of luminescence intensity, and the activity pattern of each P450 can be analyzed to detect one or more biomarkers.

The invention provides a cytochrome P450 cholesterol side chain lyase mutant, recombinant saccharomyces cerevisiae for producing progesterone and application of the recombinant saccharomyces cerevisiae. The cytochrome P450 cholesterol side chain lyase mutant is obtained through ESM-2 language model screening, and the catalytic efficiency can be remarkably improved. The efficient recombinant saccharomyces cerevisiae is obtained by constructing the recombinant saccharomyces cerevisiae, optimizing the copy number of key enzymes, introducing an electron transfer system and overexpressing the key enzymes, the yield of progesterone is remarkably increased, and the recombinant saccharomyces cerevisiae is suitable for industrial production of progesterone and has important application value.

The present invention relates to compounds of Formula (I), as well as prodrugs, pharmaceutically acceptable salts, pharmaceutical compositions, methods of use, and methods of preparation thereof. The compounds disclosed herein are useful in the treatment of diseases in which the expression of P-glycoprotein and / or cytochrome P450 (e.g., CYP3A4) is modulated (e.g., cancers that have developed multi-drug resistance).

Described herein are steroid or steroid-like compounds including at least one isonitrile group and which inhibit the activity of a cytochrome P450 enzyme, as well as compositions including the compounds. Also included are methods of inhibiting activity of a cytochrome P450 in a subject having a steroid-responsive cancer, a Mycobacterium tuberculosis infection, a fungal infection, or a trypanosome infection, the method comprising administering to the subject the compound including at least one isonitrile group or a pharmaceutically acceptable salt thereof, in an amount effective to inhibit the CYP activity in the subject.

This invention discloses a herbicide-resistant cytochrome P450 gene, BjCYP71C2, its protein, plasmid, and their applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene is SEQ ID NO:1 or has at least 80% identity with SEQ ID NO:1. This invention also discloses the protein polypeptide and plasmid encoded by the above gene. The purpose of this gene is to express it transgenic in plants, cultivating herbicide-resistant plants for selective weed control using herbicides.

The application relates to the technical field of molecular markers, and discloses an SNP site associated with turbot albinism, a molecular marker and application, wherein the SNP mutation site is a base T / A mutation at the 50th bp of a coding region of a gene KIT proto-oncogene, receptor tyrosine kinase a (kita); the SNP mutation site is a base A / T mutation at the 137th bp of a coding region of a gene cytochrome P450 3A40-like (LOC118311907); the SNP mutation site is a base G / T mutation at the 371st bp of a coding region of a gene kit ligand a (kitlga); and the SNP mutation site is a base G / A mutation at the 581st bp of a coding region of a gene frizzled class receptor 10 (fzd10). The SNP site associated with turbot albinism, the molecular marker and the application provide a precise screening method for turbot breeding. In the breeding process, the SNP molecular markers are used to determine whether the genotype is albinism or normal, to establish an excellent family with normal body color, to reduce the incidence of abnormal body color, and to improve the overall quality and economic benefits of turbot culture.

The application belongs to the technical field of diagnostic markers, and particularly relates to a pathological diagnosis biomarker combination for adrenal Cushing syndrome and application. The pathological diagnosis biomarker combination comprises low density lipoprotein receptor (LDLR), 3-hydroxy-3-methylglutaryl coenzyme A synthetase 1 (HMGCS1) and cytochrome P450 11B1 (CYP11B1). The area under the ROC curve (AUC) of the pathological diagnosis biomarker combination can reach 0.881 (95% confidence interval (CI): 0.775-987; sensitivity: 85.7%; specificity: 85.0%), which indicates that the combination of LDLR, HMGCS1 and CYP11B1 as the pathological diagnosis biomarker combination for adrenal Cushing syndrome has high accuracy.

The invention belongs to the field of chemical ecology of agricultural pests and pesticide science, and relates to an insecticide composition for improving the sensitivity of agricultural pests to insecticides and application of the insecticide composition. Folic acid is applied to a pest feeding substrate, a host plant or a feed, so that the sensitivity of pests to various insecticides (including neonicotinoids, pyrimidines, pyrethroids, organophosphorus and the like) can be remarkably enhanced. It is found that folic acid can regulate and control a carbon metabolism network in pests and inhibit cytochrome P450 and other detoxification enzyme related pathways, and then the endurance capacity of the pests to insecticides is reduced. The composition and the method are verified in seven important pests such as brown planthopper, sogatella furcifera, spodoptera frugiperda, cotton aphid, chilo suppressalis, panonychus citri and thrips, have the advantages of improving the pesticide effect, reducing the pesticide application amount and delaying the resistance evolution, and can be widely applied to comprehensive treatment of agricultural pests.

The invention discloses a W-5 strain for improving pesticide tolerance of natural enemy insects and a culture method of the W-5 strain, the W-5 strain can well grow in a lowest-salt culture medium with lambda-lambda-cyhalothrin as a unique carbon source, the OD600 of a cultured bacteria solution is continuously increased, and the degradation rate of cyhalothrin pesticide reaches 98.24%. Furthermore, after the tenebrio molitor treated by the W-5 is fed to the hunting fuscipes, the activity of two types of key detoxifying enzymes, namely glutathione S-transferase and cytochrome P450, in the body of the hunting fuscipes can be remarkably improved. After the strain is continuously fed for one week, the W-5 is successfully colonized in intestinal tracts of the hpactor fuscipes, and the survival rate of the hpactor fuscipes under the stress of lambda-lambda-cyhalothrin is increased to 90.625%. Therefore, through double mechanisms of'direct microbial degradation 'and'host detoxification metabolism activation', the W-5 synergistically enhances the resistance of the Harpactor fuscipes to the lambda-lambda-cyhalothrin.

The present disclosure provides a method of preparing an organosilicon compound having at least one silicon-bonded methanol group and / or at least one silanol group from a cytochrome P450 variant that promotes oxidation of a silicon-bonded hydrocarbon group in the presence of an oxidizing agent. The method comprises combining the cytochrome P450 variant, an initial organosilicon compound having at least one silicon-bonded hydrocarbyl group, and a cofactor to obtain a reaction mixture, and exposing the reaction mixture to an oxidizing agent to oxidize the silicon-bonded hydrocarbyl group of the initial organosilicon compound, thereby producing the organosilicon compound having at least one silicon-bonded methanol group and / or at least one silanol group. Also disclosed are cytochrome P450 variants suitable for use in the methods, as well as methods for engineering and optimizing the cytochrome P450 variants. Nucleic acids encoding the cytochrome P450 variants as well as compositions, expression vectors and host cells comprising the nucleic acids are also disclosed.

The present application relates to a cytochrome P450 oxidase, and a gene encoding the cytochrome P450 oxidase, the enzyme is involved in the final key step of the biosynthesis of important active ingredients of Tripterygium wilfordii, triptolide and / or neo-triptolide, and can be used for biosynthesis of triptolide and / or neo-triptolide.

The invention discloses a redox chaperonin PDX mutant for improving steroid hydroxylation catalysis activity of cytochrome P450 and application, according to the PDX mutant provided by the invention, the efficiency of OlePM for catalyzing LCA to generate UDCA is remarkably improved, mutants such as Y34I, Y34S, Y34E, Y34H, R67G, R67D, R67A, R67S, I90L, I90M, I90E, I90V and I90S are excellent in performance, and the UDCA concentration can reach 0.13-0.21 g / L and is improved by 5-8 times compared with that reported in literatures. By improving the electron transfer efficiency of the redox chaperonin, the steroid hydroxylation performance of the cytochrome P450 is remarkably enhanced, a new biocatalyst is provided for efficient and green biosynthesis of UDCA, and the method has important industrial application value.

The embodiment of the invention provides a cytochrome P450 hydroxylase mutant and application thereof. According to the cytochrome P450 hydroxylase mutant provided by the embodiment of the invention, a P450 enzyme single mutant CYP199A2-F185L from Rhodopseudomonas palustris is taken as a template enzyme, and the amino acid sequence of the P450 enzyme single mutant CYP199A2-F185L is shown as SEQ ID NO.1. The encoding gene of the protein is modified by adopting a site-specific mutagenesis technology, so that the encoding gene is subjected to amino acid mutation of F188W + V298L + M360W; the cytochrome P450 hydroxylase mutant provided by the embodiment of the invention can selectively catalyze hydroxylation of cinnamic acid to generate 2, 3-dihydroxycinnamic acid, has excellent biological catalytic activity and selectivity, is simple, convenient and efficient to operate, is suitable for industrial production, and has a good application prospect.

This invention provides a cytochrome P450 gene HvCYP94C1 and its application in improving the resistance of highland barley to powdery mildew, belonging to the field of agricultural biotechnology. The cytochrome P450 gene HvCYP94C1 of this invention can hydroxylate JA and JA-Ile to 12-OH-JA-Ile and 12-COOH-JA-Ile, respectively. Silencing this gene in powdery mildew-sensitive materials can significantly increase the jasmonic acid content, thereby improving the disease resistance of the materials, showing excellent application prospects.

Described herein are compositions and uses thereof comprising (a) methyl 3-((methylsulfonyl)amino)-2-(((4-phenylcyclohexyl)oxy)methyl)piperidine-1-carboxylate or a salt thereof; and (b) an agent for reducing metabolism of (a).

The invention relates to a cytochrome P450 oxidase STO1 as well as a coding gene and application thereof. According to the invention, a cytochrome P450 oxidase gene STO1 in charge of catalytic synthesis of spirovetirone in potatoes is cloned and identified for the first time, and a nucleotide sequence and a translated amino acid sequence of the gene are determined. The invention provides an expression vector, a recombinant microorganism or a transgenic cell line containing the gene. According to the invention, a saccharomyces cerevisiae engineering bacterium for producing spirovetanone is constructed, a strong promoter ERG9 in a genome of the engineering bacterium is replaced by a weak promoter HXT1, and the engineering bacterium is integrated with an STO1 gene, an AtCPR gene, a tHMG1 gene and an STS gene. The invention also provides a construction method of the engineering bacterium and a method for producing spirovetirone by using the engineering bacterium, and the engineering bacterium can be used for preparing spirovetirone.

The invention belongs to the technical field of gene detection, and particularly relates to a tetranychus urticae cytochrome P450 gene and application thereof to detection and / or monitoring of avermectin resistance of tetranychus urticae. The CYP392A13 gene shows up-regulated expression in a tetranychus urticae resistant population, and the correlation between the target gene and the avermectin resistance of the tetranychus urticae is further confirmed from the back side and the front side through RNAi and in-vitro metabolic activity tests. Therefore, the specific primer pair, the primer group, the kit and the method for detecting the abamectin resistance of the tetranychus urticae are researched and developed, if the expression quantity of a target gene in a population to be detected is obviously higher than that of a tetranychus urticae sensitive population, the population to be detected has the abamectin resistance; a key molecular tool is provided for establishing an abamectin resistance early warning system, and a drug resistance treatment strategy can be formulated in time.

The invention belongs to the technical field of biology, and relates to a cytochrome P450 mutant and application thereof. The mutant is obtained by implementing site-directed mutagenesis of F87A, F81I and A82F on an amino acid sequence shown as SEQ ID NO. 2 in a sequence table. The cytochrome P450 mutant provided by the invention shows high catalytic activity on an unnatural substrate epothilone D, and efficient conversion of the epothilone D is realized. The mutant can catalyze the hydroxylation reaction of epothilone D at a plurality of sites (such as C-21 and C-26) to generate at least four different derivatives, thereby providing a precious compound library for drug screening.

The invention discloses a recombinant saccharomyces cerevisiae strain with high yield of asiatic acid and a construction method of the recombinant saccharomyces cerevisiae strain. The method comprises the following steps: integrating a C-2alpha hydroxylated cytochrome P450 oxidase gene AmCYP716C53 from avicennia marina and a C-23 hydroxylated cytochrome P450 oxidase gene CaCYP716E19 from centella asiatica to a chromosome 911b site of a chassis strain ZY35 by utilizing a CRISPR-Cas9 gene editing technology, so as to obtain ZSA01; then, genes MCH5, FLX1, RIB1, FMN1 and FAD1 are integrated to the 1014a site of the ZSA01, and ZSA02 is obtained; finally, the AmCYP716C53 is integrated to the HMX1 site of the ZSA02, and an engineering strain ZSA03 is constructed. Fermentation results show that ZSA03 reaches 1g / L in a 5L fermentation tank, and the ZSA03 has high-efficiency synthesis and industrial application potential.

The invention provides optimization and application of elements in a mogroside biosynthesis pathway, and particularly, after key sites in epoxy hydrolase EPH and cytochrome P450 are modified, the yield of 24, 25-dihydroxy cucurbitadienol and mogrol can be remarkably increased, and the yield of by-products can be reduced.

The application discloses a molecular marker for resistance of a citrus psyllid to thiamethoxam and application and detection method thereof. The application discovers the molecular marker for resistance of the citrus psyllid to thiamethoxam, namely, cytochrome P450 genes DcitCYP6K3, DcitCYP4V1, DcitCYP15A4 and DcitCYP301a2, through synergistic experiments, enzyme activity determination and expression profile analysis. The molecular marker has high specificity and stability, and through analysis of relative expression amounts of the molecular marker of the citrus psyllid to-be-tested sample, it can be known whether the to-be-tested sample is a sensitive strain or a resistant strain to thiamethoxam, so that the drug resistance of the citrus psyllid can be monitored at any time, early monitoring of the drug resistance of the citrus psyllid is facilitated, and the application has important significance for continuous and effective control of the drug resistance of the citrus psyllid and prevention and treatment of Huanglongbing.

The present invention relates to a novel quinoline or quinazoline compound and the use thereof. The compound according to the present invention shows an excellent inhibitory activity against cytochrome P450 4A (CYP4A) and thus may be utilized for preventing, relieving or treating metabolic diseases.

The present invention provides compounds for the regulation of P-glycoprotein and cytochrome P450 (e.g., CYP3A4 and CYP3A5 isoforms) enzymes, which a) significantly improve the bioavailability of substrates of these enzymes, including anticancer drugs, b) overcome multidrug resistance in tumors, and c) reduce serious side effects while improving the delivery of P-glycoprotein substrates to the brain. [Solution] Compounds of formula (I), as well as their prodrugs and pharmaceutically acceptable salts, are provided. Further disclosure relates to pharmaceutical compositions containing the compounds, methods of use, and methods for preparing them. TIFF2026086798000135.tif39128

The application belongs to the technical field of medicine, and specifically provides an entero-soluble ibrexin tablet with high bioavailability and a preparation method thereof. The entero-soluble tablet comprises a tablet core and an enteric coating, wherein the tablet core comprises an ibrexin coating compound, microcrystalline cellulose and magnesium stearate, and the ibrexin coating compound comprises ibrexin or a pharmaceutically acceptable salt thereof, an acid regulator, povidone and cross-linked sodium carboxymethyl cellulose. The ibrexin entero-soluble tablet of the application can make the drug reach the end of the small intestine or the colon for release, thereby avoiding the metabolism of cytochrome P450 (CYP) 3A, and at the same time, the acid regulator can locally provide an acidic environment for the active ingredient, so that the ibrexin is rapidly dissolved and released, and the side effects such as vomiting in the gastrointestinal tract are reduced.

The invention belongs to the technical field of biology, and particularly relates to a metolachlor and chloroacetamide herbicide degrading bacterium, an enzyme and application. According to the present invention, the degrading bacterium Rhodococcus sp. L-9 capable of efficiently degrading metolachlor and other chloroacetamide herbicides is found through screening, and the degrading bacterium Rhodococcus sp. L-9 can be used for efficiently degrading metolachlor and other chloroacetamide herbicides. Furthermore, according to the invention, a gene cluster metABD for degrading the metolachlor and other chloroacetamide herbicides is cloned from a strain L-9, and the gene cluster metABD can encode three-component cytochrome P450 oxidase MetABD to catalyze the degradation of the metolachlor and other five chloroacetamide herbicides (alachlor, acetochlor, propisochlor, pretilachlor and butachlor); and enantiomer selectivity is achieved in catalysis of chiral herbicide metolachlor, and good social and economic benefits are achieved.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method based on a cytochrome P450 gene CYP9G2 and a matched kit, which are used for rapidly and accurately detecting the drug resistance of plutella xylostella to emamectin benzoate. According to the method, whether a plutella xylostella individual or population has resistance to emamectin benzoate or not is judged by quantitatively analyzing the mRNA expression level of a CYP9G2 gene. The kit comprises a specific primer pair, and the sequences of the specific primer pair are respectively SEQ ID NO.1 (forward) and SEQ ID NO.2 (reverse). The method is easy and convenient to operate and rapid in detection, sample treatment to result analysis can be completed within 3-4 hours, and the method is remarkably superior to a traditional biological determination method. The application of the method can realize early discovery and dynamic monitoring of the drug resistance of field plutella xylostella, provides a direct basis for scientific use and resistance treatment of emamectin benzoate in vegetable production, is beneficial to reduction of the pesticide use amount, reduction of the control cost and guarantee of the vegetable quality safety, and has important practical popularization value in the aspects of agricultural green production and pesticide application reduction.

A method for preparing an organosilicon compound having at least one silicon-bonded carbinol group and / or at least one silanol group, having a cytochrome P450 variant that promotes oxidation of a silicon-bonded hydrocarbyl group in the presence of an oxidizing agent. The method comprises: combining a cytochrome P450 variant, an initial organosilicon compound having at least one silicon-bonded hydrocarbyl group, and a cofactor to obtain a reaction mixture; and exposing the reaction mixture to an oxidizing agent to oxidize the silicon-bonded hydrocarbyl group of the initial organosilicon compound, thereby preparing an organosilicon compound having at least one silicon-bonded carbinol group and / or at least one silanol group. Cytochrome P450 variants suitable for use in this method are also disclosed, along with methods for manipulating and optimizing them. Nucleic acids and compositions encoding cytochrome P450 variants, expression vectors, and host cells containing them are also disclosed.

The present application relates to a cytochrome P450 gene and its encoding product related to cucurbitadienol hydroxylation, and belongs to the field of genetic engineering. The nucleotides of the gene are shown in SEQ ID NO: 1-5. The present application also provides an encoding protein related to the gene, a vector, an engineering bacterium and application.