70 results about "Conserved sequence" patented technology

The invention discloses a method and a device for identifying conservative non-coding elements (CNEs) in a genome. The method comprises the following steps: respectively comparing other genomes in a plurality of high-quality genomes with a reference genome to obtain genome comparison results; identifying the comparison result of the species evolutionary relationship topological structure and the genome to obtain a conserved sequence position information gff file; the gff file is filtered, and a CNEs.gff file is obtained; a CNEs sequence is extracted from the CNEs.gff file; determining a CNEs differentiation sequence according to the CNEs sequence; and performing function enrichment analysis on the target gene of the CNEs differentiation sequence to obtain a plurality of identification results of the CNEs in the high-quality genome. According to the method and the device, the technical problem that identification of the CNEs is incomplete or misjudged due to the fact that identification of the CNEs is often influenced by genome structure variation in the prior art is solved.

This disclosure provides, in some aspects, multi-component trans-amplifying RNA (taRNA) vaccines that comprise a first nanoparticle (R-NP) comprising a polynucleotide encoding a replicase and a second nanoparticle (Tr-NP) comprising a polynucleotide comprising a nucleic acid encoding a payload (e.g., antigen), operably linked to a conserved sequence element (CSE) that is cognate to the replicase; methods of production thereof; and methods of use thereof.

The invention provides a PCR (polymerase chain reaction) method for rapidly screening effective donors for FMT (fibronectin) treatment of depression, which comprises the following steps: (1) collecting excrement samples of the donors, and extracting total microbial genome DNA (deoxyribonucleic acid) of the excrement samples; (2) designing six primer pairs according to a specific conserved sequence of a target strain; (3) taking the extracted donor excrement DNA as a PCR (Polymerase Chain Reaction) template, and adding a primer pair for amplification; (4) observing a PCR result through electrophoresis; and if each target band is positive in the PCR electrophoresis result of the donor sample, determining that the donor contains the target strain, and determining that the donor is an effective donor for FMT treatment of depression. The effective donor suitable for FMT treatment of depression is screened out by detecting whether the donor faeces contain three key probiotics closely related to depression symptom relieving or not, a basis is provided for microbial therapy of mental diseases such as depression, and important clinical application value is achieved.

The invention discloses a germacene A synthase mutant and application thereof, and belongs to the technical field of enzyme engineering and microbial engineering. Aiming at the bottleneck of the catalytic efficiency of the germacene A synthase, the optimal germacene A synthase mutant DlGASS32H-I97V-Y315F is obtained through semi-rational design based on sequence conservative analysis by taking the germacene A synthase from fungi with a separation wheel layer carbon shell as an object, and the optimal germacene A synthase mutant DlGASS32H-I97V-Y315F is verified through shake flask fermentation in engineering bacteria saccharomyces cerevisiae, so that the yield of beta-elemene is increased by 24%. The combined mutant obtained through a key gene conserved sequence mutation prediction strategy shows remarkably improved catalytic performance, the effectiveness of guiding an enzyme directed evolution strategy based on multi-species sequence conservative analysis is verified, and the yield of germacene A or beta-elemene is remarkably improved. Meanwhile, the method has the advantages of simplicity and convenience in operation, environmental friendliness, mild reaction conditions and the like, and has a very good application prospect in the field of production of germacene A.

The invention belongs to the technical field of meat product detection, particularly relates to a kit for detecting animal meat-derived components based on RPA-CRISPR-Cas12a and a detection method, and provides a combination of a primer pair suitable for isothermal amplification and a CRISPR / Cas12a nucleic acid detection system and a gene conserved sequence capable of specifically recognizing common meat-derived components. According to the method, RPA and CRISPR technologies are combined, the RPA primer and the crRNA sequence for detection are screened and optimized, the nucleic acid detection test strip and naked eye fluorescence color development are combined, meat-derived components in a sample can be rapidly identified without special professional equipment in the field detection process, and the method is suitable for rapid field detection of a base layer.

The invention relates to a nucleic acid detection platform based on an SHERLOCK technology, which is used for diagnosing CAV-2. The SHERLOCK consisting of recombinase polymerase nucleic acid pre-amplification (RPA) and CRISPR-Cas13a is mainly used for specifically recognizing a conserved sequence of CAV-2RNA (Ribonucleic Acid) through crRNA (Complementary Ribonucleic Acid) so as to activate the non-specific'side cutting activity 'of lwCas13a, so that the report RNA is degraded. A real-time fluorescent quantitative PCR (qPCR) system or a lateral flow test strip is used for collecting fluorescent signals generated by cracking of the reporter molecules, and a visual result is displayed. In addition, through HUDSON treatment, RPA-CRISPR can be amplified at room temperature without viral nucleic acid extraction. The detection limit of the method is 1.1 * 10 < 2 > copies / [mu] L, and the method has no cross reaction with other canine infectious viruses. From sample collection to result output, the whole reaction of RPA-CRISPR can be completed within two hours, and the RPA-CRISPR kit is suitable for rapid test of clinical samples and can work well in the field or in primary laboratories. The method can get rid of the limitation of a detection instrument and complex operation, and is suitable for on-site rapid batch detection. The method is preliminarily verified and further optimized and applied.

The invention relates to the technical field of bioinformatics, in particular to a method for identifying an abnormally hypermethylated region in cancer methylation data. The method comprises the following steps: acquiring whole genome methylation sequencing data of a sample to be detected and genome conserved sequence data of primate species; performing density analysis based on a progressive hierarchical scanning strategy on the CpG loci according to the whole genome methylation sequencing data to obtain CpG enriched region data; performing distance calculation including short-range, medium-range and long-range on the genome conservative sequence data, and performing weighted correction on a calculation result according to the CpG enrichment region data to obtain region conservative score data. According to the method, through unsupervised clustering, self-adaptive threshold setting and multi-scale feature extraction, high methylation regions with high regulation and control effects in different cancer types can be more accurately captured.

The invention provides a primer for detecting a novel bunyavirus by an RPA-lateral flow nucleic acid chromatography technology, which comprises a specific SFTSV primer pair marked by a nucleic acid sequence, namely SFTSV-F and SFTSV-R. The invention further provides a kit for detecting the novel bunyavirus by the RPA-lateral flow nucleic acid chromatography technology. The invention also provides a probe which comprises a gold nanoparticle capture probe, an SFTSV capture probe and a quality control capture probe. Meanwhile, the invention further provides application of the primer and the probe in preparation of SFTSV detection products, a specific detection kit and a specific detection method. The specific primer and the functional differentiation probe are designed aiming at a novel bunyavirus highly-conserved sequence, and an RPA isothermal amplification technology and a lateral flow nucleic acid chromatography visual detection technology are integrated, so that the rapid, sensitive and specific detection of the SFTSV is realized, the dependence on a large instrument is not needed, the detection cost and the operation threshold are reduced, and the detection efficiency is improved. And the application requirements of on-site rapid detection, large-scale screening and resource-limited regions are met.

The invention belongs to the technical field of virus detection, and particularly relates to an anguilla positive reovirus (Anguilla positive reovirus) AORV-1025 as well as a primer group, a kit and a method for detecting the anguilla positive reovirus. According to the invention, a conserved sequence of an RdRp gene coded by an L3 segment of an eel positive reovirus genome is taken as a target, a primer group consisting of a primer pair and a probe is designed, and the primer group is used for detecting several different positive reoviruses including the eel positive reovirus and viruses possibly existing in a detection sample. The detection result is high in sensitivity, strong in specificity and excellent in repeatability, the eel positive reovirus in a sample can be rapidly and accurately detected in a fluorescent quantitative mode, the method is applied to research on eel positive reovirus infection, and the method has important significance on pathogen monitoring, differential diagnosis and guide prevention and control of eel viral diseases.

This invention provides a method for identifying abamectin resistance genes in the fall armyworm, dsRNA, and their applications, belonging to the field of molecular biology. This invention combines genome resequencing and transcriptome sequencing to identify key abamectin resistance genes in the fall armyworm. Based on the conserved mRNA sequences of the key resistance genes GSTD6 and CTP6A18, dsRNA was designed, and RNAi technology can effectively reduce the fall armyworm's resistance to abamectin. This has significant practical implications for developing effective fall armyworm resistance management strategies to delay the emergence of resistance and for identifying new targets for pest control.

The invention provides a target, a primer and a crRNA composition for a bovine rotavirus RT-RAA-CRISPR / Cas12a detection method, and belongs to the technical field of nucleic acid visual detection. A primer and a cRNA composition are designed on the basis of a conserved sequence in BRV VP6, an RT-RAA technology is combined with a CRISPR / Cas12a system, precise variable temperature equipment is not needed, effective amplification of a BRV target gene can be obtained only under the constant temperature condition, and the problems that an existing BRV detection method needs to be improved in sensitivity, the requirements for the operation environment and precise equipment are high, and the detection cost is low are solved. And rapid detection of the BRV cannot be realized in an epidemic disease site or a basic laboratory.

This invention belongs to the field of bioengineering technology and discloses a method for specific targeting and scarless simultaneous elimination blue KPC‑2 Gene and IncL plasmid sgRNA sequences, CRISPR / Cas9 vector system and applications. This system targets the sgRNA of the IncL plasmid. blue KPC‑2 The IncL-type plasmid of the gene was designed with an sgRNA that specifically recognizes the conserved sequence at its origin of replication, thus eliminating the drug resistance gene while disrupting the plasmid's replication ability. Conjugation transfer to recipient bacteria significantly improved vector delivery efficiency. An arabinose-inducible promoter was used to control Cas9 gene expression, improving system stability and editing success rate. The vector carries a sucrose-inducible self-eliminating gene, enabling controlled vector self-elimination. This system is not a simple superposition but rather constitutes a time-controlled operational flow: efficient delivery via conjugation, controlled cleavage via induction, and finally, induced self-elimination.

The invention relates to the technical field of construction of conditional gene knockout models, and discloses a construction method of a mouse Oct4 conditional gene knockout model based on quadruple sgRNA collaborative targeting, which comprises the following steps: step 1, designing four sgRNAs, namely sgRNA1, sgRNA2, sgRNA3 and sgRNA4, in which the sgRNA1 and the sgRNA2 target 5'end and 3 'end non-coding regions of a second exon of an Oct4 gene, and the sgRNA3 and the sgRNA4 target structural domain conserved sequences; step 2, preparing an electrotransfection solution, wherein the electrotransfection solution is composed of Cas9mRNA, the four sgRNAs and an electrotransfection buffer solution; step 3, carrying out electroporation treatment on the fertilized eggs of the mice; step 4, culturing the fertilized eggs subjected to the electrotransformation treatment to a blastocyst stage; and 5, carrying out genotype identification on the cultured blastocyst. According to the scheme, the sgRNA combination and embryo electrotransfection system is optimized, the mouse Oct4 conditional gene knockout model is efficiently and accurately constructed, a reliable tool is provided for regulation mechanism research of Oct4 in mouse early embryo trigerm differentiation, and the medical transformation value of Oct4 is excavated.

The invention provides a fluorescent quantitative PCR (polymerase chain reaction) detection primer, a kit and a method for a novel variable chalmavirus VP gene, and belongs to the technical field of virus detection. A specific primer is designed and synthesized according to a conserved sequence of a VP gene of the novel variant chalmavirus, a recombinant plasmid is constructed, then the recombinant plasmid is used as a standard substance to establish a standard curve, a real-time fluorescent quantitative PCR detection system aiming at the novel variant chalmavirus is constructed, and specific detection of the novel variant chalmavirus is realized. The detection method can be used for quantitatively detecting the novel variant chalmavirus in the to-be-detected sample, and has the characteristics of high speed, high sensitivity, strong specificity and the like; the method provides a key technical support for molecular epidemiological investigation of the Sepamavirus in animal populations, is helpful for realizing early screening of infected individuals and timely blocking of virus transmission, and has important practical significance for guaranteeing population health and maintaining high mountain ecosystem stability.

The invention provides a real-time fluorescent quantitative PCR (polymerase chain reaction) detection primer, kit and method for novel variant bocavirus, and belongs to the technical field of virus nucleic acid detection. A specific primer is designed and synthesized according to a conserved sequence of a novel variant bocavirus (BoV) VP gene, a recombinant plasmid pcDNA3.1 HA-BoV-VP is constructed, then the recombinant plasmid is used as a standard substance to establish a standard curve, a real-time fluorescent quantitative PCR detection system for the novel variant bocavirus is constructed, and specific detection of the novel variant bocavirus is realized. The detection method can realize quantitative detection of bocavirus in a to-be-detected sample, has the characteristics of high speed, high sensitivity, strong specificity and the like, plays an important role in protection of endangered wild animals such as disc sheep, and has good practicability.

The invention discloses a primer combination for detecting Ralstonia pickettii and a kit for detecting the Ralstonia pickettii, wherein the kit comprises the primer combination. The primer probe provided by the invention is targeted to a specific nucleotide sequence of the Ralstonia piceatrii instead of a horizontal conserved sequence, so that the Ralstonia piceatrii and other strains in the Ralstonia can be effectively distinguished, the technical defects that an existing detection method is insufficient in specificity and easy to generate false positive are overcome, and the accuracy of a detection result is improved. The detection limit can reach 100 CFU / mL, and compared with the prior art, the detection limit is reduced, and the detection sensitivity is improved. The kit disclosed by the invention can be applied to pollution detection of medical supplies such as pharmaceutical water, dialysis water, endoscope cleaning water, blood products, sterile normal saline and the like and rapid screening of hospital infection, is strong in specificity and high in accuracy, and has relatively high popularization and application values.

The invention discloses a rift valley fever virus nucleic acid RT-RAA-CRISPR / Cas12a detection primer probe combination, a kit and a method, and belongs to the technical field of molecular detection. In order to solve the problems that an existing RVFV detection technology is high in equipment dependence, low in sensitivity, not suitable for primary laboratories and epidemic disease sites and the like in clinical application, a highly conserved sequence of an S gene of an RVFV ZH-548 strain is selected as a detection target through comparative analysis of an RVFV S gene sequence, and the RVFV ZH-548 strain S gene is detected on the basis of the target. A group of primers and probes for RVFV nucleic acid RT-RAA-CRISPR / Cas12a visual detection are researched and developed, a corresponding detection method is established, and a matched detection kit is provided. The detection method provided by the invention is high in sensitivity, strong in specificity, simple and convenient to operate and high in safety, and has remarkable advantages in scenes such as cross-border animal quarantine, epidemic area field emergency detection and grassroots pathogen rapid screening.

The invention provides a ZmSWEET4 gene related to a corn smut disease and application of the ZmSWEET4 gene in regulation and control of the corn smut disease, and belongs to the technical field of gene engineering. The invention provides a ZmSWEET4 gene capable of regulating and controlling the resistance of corn to the tumor smut disease. A coded protein contains conserved sequences as shown in SEQ ID No.1 and SEQ ID No.2. The invention also provides a preparation method of the ZmSWEET4 gene capable of regulating and controlling the resistance of corn to the tumor smut disease. According to the present invention, the ZmSWEET4a gene and the ZmSWEET4b gene in the receptor corn are respectively edited to obtain the transgenic plant in which the ZmSWEET4a gene and the ZmSWEET4b gene are respectively knocked out, and compared with the wild type corn, the gene knockout plant has characteristics of more disease resistance. The invention proves that the ZmSWEET4 gene is related to the resistance of corn to Ustilago esculenta for the first time, and can be used for creating new germplasm resistant to the corn Ustilago esculenta and developing or screening medicines capable of preventing and / or treating the corn Ustilago esculenta.

The invention belongs to the technical field of biological genetic engineering, and discloses a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) system for specific targeted traceless elimination of a blaNDM-1 gene and an IncN plasmid and application of the CRISPR / Cas9 system. According to the system, sgRNA of an original point conserved sequence is copied by designing an IncN type plasmid carrying a blaNDM-1 gene in a targeting manner, so that the replication capability of the plasmid is destroyed while a drug-resistant gene is eliminated; the carrier delivery efficiency is greatly improved by conjugational transfer to recipient bacteria; the expression of the Cas9 gene is controlled by adopting an arabinose inducible promoter, so that the constitutive toxicity of Cas9 protein is reduced, and the system stability and the editing success rate are improved; the sucrose-induced carrier self-elimination gene carried by the carrier realizes controllable self-elimination of the carrier. The system is not simply superposed, but forms an operation process with a controllable time sequence: efficient delivery is firstly carried out through joint, then cutting is controlled through induction, and finally self-elimination is induced.

The invention discloses a tomato FtsH5 gene mutation method and application, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: designing a target sequence according to a CDS conserved sequence of the tomato FtsH5 gene to construct a CRISPR-Cas9 genetic transformation vector, constructing recombinant agrobacterium tumefaciens to transform tomato, performing PCR detection sequencing to obtain an FtsH5 gene knockout tomato plant, and performing offspring PCR identification and screening to obtain FtsH5 gene heterozygous mutant and homozygous mutant seeds. Cultivation and planting results show that the homozygous mutant tomato is albino and dead, and the heterozygous mutant leaves generate stable albino spots. Experiments show that the genetic shape of the FtsH5 gene knockout tomato hybrid strain is stable, the randomness defect of a traditional mutagenesis technology is avoided, and the FtsH5 gene knockout tomato hybrid strain can be used for viewing and follow-up chloroplast and photosynthetic efficiency related research.

The present application relates to the technical field of plant gene function, and aims to provide a construction method of a homologous silencing system of a Paeonia lactiflora bud dormancy gene, comprising the following steps: designing amplification primers according to gene annotation and a conserved sequence region in a Paeonia lactiflora bud dormancy full-length transcriptome, and constructing a conserved sequence fragment of a target gene into Agrobacterium tumefaciens containing a pTRV vector; taking a rhizome of a treated Paeonia lactiflora annual seedling as an infection material, and placing the infection material in Agrobacterium tumefaciens liquid containing pTRV1, pTRV2 and pTRV2-target gene fragments to perform infection treatment; potting the rhizome and moving the rhizome into a culture room to perform culture, observing phenotype and expression amount differences between the rhizome and a control group in a growth process, and confirming that the homologous silencing system is successfully constructed. The present application first completes miniaturization treatment and batch bacterial liquid infection of an underground rhizome system of Paeonia lactiflora, establishes a virus-mediated homologous function silencing system of a Paeonia lactiflora bud dormancy gene, and realizes the purpose of verifying functions of bud dormancy and even germination and growth related genes.

The invention discloses a kit for detecting toxin-producing vibrio cholerae based on a dual-fluorescence RPA (recombinase polymerase amplification) technology and application of the kit. The invention provides a complete set of primers and probes. The complete set of primers and probes comprises a primer Vc-F1, a primer Vc-R4, a probe Vc-P, a primer ctxB-F, a primer ctxB-R and a probe ctxB-P, according to the invention, countless tests are matched with specific retrieval, a section of specific conserved sequence Vc and virulence gene ctxB on a pathogen genome are selected as detection targets, and whether a to-be-detected sample is toxigenic vibrio cholerae or not can be rapidly determined by simultaneously detecting the two targets. According to the invention, a brand new technical support is provided for on-site rapid diagnosis and epidemic disease investigation of vibrio cholerae by applying a dual-fluorescence RPA technology.

The invention discloses a fluorescent MIRA kit and method for detecting a monkey varicella virus, and relates to the technical field of biology.The kit comprises a primer probe combination, a buffer solution A, a buffer solution B, freeze-dried enzyme powder and a nucleic acid extraction.The primer probe combination is optimally designed and synthesized on the basis of a monkey varicella virus ORF21 gene conserved sequence; according to the present invention, the monkey varicella virus detection method is established and optimized, and the reaction system is further constructed, such that the optimal reaction condition and the optimal primer pair are determined, the monkey varicella virus detection method is established and optimized, and the constructed detection method has advantages of high sensitivity, good specificity, simple operation, fast detection speed, low cost, and wide application prospect. The kit has an important application value in detecting whether a sample contains the monkey varicella virus or not.

The invention discloses a recombinant protein as well as a coding gene and application thereof, and belongs to the technical field of bioengineering. The invention designs a recombinant protein which is composed of two subunits with different functions, and design and transformation are carried out on the basis of a conserved sequence of an alpha herpes virus protein with an immune escape function, so that the secretory expression of the recombinant protein is promoted, and the defects of inclusion body formation, overhigh host toxicity and high target protein purification difficulty are overcome. Experimental results show that the recombinant protein provided by the invention has a remarkable effect of inhibiting innate immunity of cells, and can effectively inhibit interferon generated by innate immunity of cells, so that the titer of cultured viruses is improved.

The invention relates to the field of biological detection, in particular to a primer, a probe, a detection method and a kit aiming at chikungunya virus. The primer pair and the probe take the conserved sequence of the chikungunya virus nsP1 gene as a target, are suitable for detecting the chikungunya virus through an RT-RPA-LFD method, and can realize accurate, rapid and efficient detection of the chikungunya virus.

The invention discloses a quadruple fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for simultaneously detecting multiple pathogens in bovine red blood cells. The method comprises the following steps: acquiring core functional gene sequences of four pathogens; carrying out gene sequence comparison, and screening out continuous conservative fragments; comparing the conserved sequence with the bovine genome and the homology of the microorganisms to obtain a specific conserved sequence; designing four pairs of PCR primers with the same annealing temperature, and designing a probe; selecting a gene sequence fragment, treating the gene sequence fragment with restriction enzyme, and connecting a target gene fragment with a T vector by using DNA (Deoxyribose Nucleic Acid) ligase; transforming the connection product into competent cells for culture, determining successful cloning, and extracting plasmids; the PCR primer and the probe are tested for multiple times, and the annealing temperature and the component concentration of a reaction system are adjusted. By implementing the method disclosed by the invention, babesia, theileria, anaplasma and eperythrozoon can be detected at the same time, the diagnosis efficiency is remarkably improved, and the risk of missed diagnosis is reduced.

The invention discloses a primer group, a probe, a reagent and a kit for detecting cryptosporidium based on an RAA technology and a detection method and application of the primer group and the probe. The primer group and the probe are designed based on a highly conserved sequence of the cryptosporidium and are high in sensitivity, the lowest detection limit can reach 4 copies / mu L, the specificity is high, only specific amplification is achieved on the cryptosporidium, and the detection time is short. The cryptosporidium detection method is established on the basis of a cryptosporidium RAA amplification method, expensive and complex large-scale instruments and equipment and professional laboratory equipment are not needed, and the test results of 46 clinical samples show that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result is accurate. After reaction at 39 DEG C for 20 minutes, visual result judgment can be carried out under the irradiation of a fluorescence detector or a blue light instrument. The method is of great significance to detection, prevention and control of cryptosporidium.

The present invention relates to the field of molecular biology detection technology, and specifically to a probe cluster, a kit and its application in detecting Clostridium difficile, wherein the kit includes a recombinase-mediated isothermal nucleic acid amplification system, amplification primers of the conservative sequence of the toxin B gene of Clostridium difficile, four sets of locked nucleic acid-modified G-quadruplex / heme DNA enzyme probes and competitive probes. In this method, nucleic acid amplification and its hybridization with the probe are carried out under constant temperature (42°C-45°C), and there is no need to purify the amplified product, and the steps are few and the speed is fast, and all detections can be completed within 40 minutes. The method has high sensitivity, good specificity, does not rely on complex instruments, and the results can be directly observed with the naked eye, which is suitable for the rapid detection of Clostridium difficile.

The application belongs to the field of biological detection, and particularly relates to an isothermal amplification kit for detecting porcine circovirus type 3. The application provides an isothermal amplification detection primer group for porcine circovirus type 3 (PCV3), which comprises a pair of outer primers (LOF and LOR) and a pair of inner primers (LIF and LIR) and is designed according to the conserved sequence of the porcine circovirus type 3. The primer group has low design difficulty, and maintains similar sensitivity and specificity to other isothermal amplification reactions such as a loop-mediated isothermal amplification method, can be suitable for target sequences with higher GC content and below 200 bp, has no denaturation and annealing process, is suitable for on-site detection, and has great application potential.

The invention provides a detection method for detecting Sf9 host DNA residues by using a fluorescent probe and application, and belongs to the technical field of biological detection.According to the method, a specific primer pair (such as SEQIDNO: 1 / 2) and the fluorescent probe (such as SEQIDNO: 11) are designed according to a high-copy conserved sequence of Sf9 host DNA, a kit containing a gene amplification solution, a standard substance and a quality control substance is matched, DNA is extracted through a liquid nitrogen grinding-column extraction method, and the detection result is accurate. And carrying out real-time fluorescent quantitative PCR amplification and then carrying out detection. According to the method, the detection sensitivity reaches 0.003 pg / mu L, the linear range is 300 pg / mu L-0. 003 pg / mu L, cross reaction to irrelevant DNA is avoided, the repeatability CV% is smaller than or equal to 10%, the reagent is still stable after being frozen and thawed for 10 times, and the whole process is completed within 1.5 hours; the method solves the problems of insufficient specificity and sensitivity, tedious operation and the like of the existing method, and can be used for quality control of biological products such as gene therapy drugs and cell vaccines.