296 results about "Biotransformation" patented technology

The invention relates to the technical field of health care products, and discloses kidney function health wine based on traditional Chinese medicine fermentation and a preparation method of the kidney function health wine. 6-10 parts of cistanche deserticola; 9-30 parts of radix astragali and 6-12 parts of fructus lycii; the fermentation strain comprises lactobacillus acidophilus, lactobacillus plantarum, bifidobacterium longum subsp. Longum, saccharomyces cerevisiae and bacillus subtilis, and the fermentation strain comprises the following strains: lactobacillus acidophilus JYLC-374, lactobacillus plantarum JYLC-482, bifidobacterium longum subsp. Longum JYBB-163, saccharomyces cerevisiae JYSC-710 and bacillus subtilis JYBS-312. By adopting the technical scheme that a traditional Chinese medicine compound formula is combined with specific probiotic fermentation, the technical effects of enhancing the bioavailability of active ingredients and promoting kidney function regulation are achieved, and the dissolution rate and the biotransformation rate of herbal medicine active substances are improved.

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention belongs to the field of biological pharmacy and biological conversion, and particularly relates to a lipase mutant and application thereof. The lipase CALB mutant disclosed by the invention is obtained by carrying out single-point mutation or combined mutation on the 40th site, the 134 site, the 154 site, the 189th site, the 278th site and the 281 site of an amino acid sequence as shown in SEQ ID NO.1. Compared with a wild type, the mutant has the advantage that the stereoselectivity is greatly improved when the mutant is used for catalyzing racemization 3-cyclohexene-1-methyl formate to produce (S)-3-cyclohexene-1-formic acid. Compared with a traditional chemical method for preparing (S)-3-cyclohexene-1-formic acid, the lipase CALB mutant provided by the invention is high in stereoselectivity, mild in reaction condition, environment-friendly, low in equipment requirement, capable of greatly reducing the production cost and wide in application prospect in industrial application.

The invention discloses a method for efficient conversion and utilization of mogroside. According to the method, mogroside V is converted through fermentation of candida parapsilosis L7, and siamenoside I is directionally synthesized. According to the invention, Candida parapsiloides L7 is utilized for fermentation conversion, and beta-1, 6-glucosidic bonds in glucose branched chains connected with C3-site hydroxyl of a mogroside V structure parent nucleus can be directionally hydrolyzed with high regioselectivity (the beta-1, 6-glucosidic bonds on glucose branched chains connected with C24-site hydroxyl are hardly hydrolyzed), so that one molecule of glucose is removed, and siamenoside I is obtained. The siamenoside I provided by the invention can be efficiently prepared, and the maximum molar yield of the siamenoside I can reach 87.40%. The siamenoside I has more obvious selective hydrolysis characteristic, higher space time yield of the siamenoside I, no cytotoxicity of the siamenoside I and better anti-inflammatory and antioxidant activity, provides a new drug choice for diseases such as inflammation and the like, and provides technical support for development of high-valued products of mogroside.

The invention discloses a hermetia illucens breeding method for improving the biotransformation capacity of hermetia illucens on agricultural wastes. The hermetia illucens breeding method capable of improving the biotransformation capacity of the hermetia illucens on the agricultural waste comprises the following steps: inoculating larvae of the hermetia illucens into the agricultural waste added with Penicillium nucleatum, breeding, and collecting insect sand and insect bodies. The method disclosed by the invention can be used for improving the biotransformation capability of the hermetia illucens on the agricultural wastes, which is specifically embodied in the aspects of increasing the waste reduction rate, increasing the biotransformation rate, increasing the biomass transformation ratio of the substrate and the hermetia illucens prepupae, increasing the growth rate of the hermetia illucens, increasing the dry matter content and the protein content of hermetia illucens bodies and the like; the method provided by the invention can realize rapid and efficient degradation of wastes, reduces resource loss and environmental pollution, provides a brand new solution for waste treatment, and comprehensively improves the resource utilization efficiency.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant, a coding gene and application. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant as well as a coding gene and application thereof. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention provides an alkene reductase mutant and an application of the alkene reductase mutant in synthesis of (R)-2-dimethyl methylsuccinate. According to the method, the olefin reductase is subjected to directed evolution to obtain the olefin reductase mutant with improved enzyme activity and substrate tolerance, the obtained mutant can be used for efficiently reducing dimethyl itaconate to prepare the high-optical-purity (R)-2-dimethyl methylsuccinate, and the reaction substrate concentration, the reaction yield and the optical purity of the product are high; the reaction process is simple to operate and low in energy consumption, and meets green chemical requirements. Therefore, the method disclosed by the invention can be applied to the biotransformation of the industrial production of the (R)-2-methyl dimethyl succinate.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention belongs to the technical field of microorganisms, and particularly discloses a fluoroquinolone antibiotic efficient degrading bacterium and application thereof. The fluoroquinolone antibiotic degrading bacterium is Kocuria sp. X03, the preservation number of the Kocuria sp. X03 is CCTCC NO: M 2025522, the invention provides the fluoroquinolone antibiotic degrading bacterium Kocuria sp. X03 separated from soil, the Kocuria sp. X03 can efficiently degrade fluoroquinolone antibiotics, an antibiotic degrading strain library is enriched, biotransformation mechanism research and bioremediation application are promoted, and the application prospect is wide. The strain can be used for degrading and eliminating livestock and poultry manure and residual antibiotics in farmland soil, and has certain research and application values.

The invention relates to the technical field of soybean milk products, in particular to application of a soybean folic acid enrichment method in soybean milk rich in folic acid. According to the method disclosed by the invention, a plant biotransformation technology is utilized, and folic acid in the soybeans is enriched by cleaning and soaking the soybeans, adding a substrate and a hypertonic substance and regulating and controlling the temperature and activating folic acid synthetase. The preparation method comprises the following steps: germinating soybeans for 24-36 hours, homogenizing, adding wheat germs and an additive, boiling the soybean milk, blending, homogenizing and sterilizing to obtain a stable healthy product with high folic acid content.

The invention discloses a fermentation preparation method of a high-COD (Chemical Oxygen Demand) composite carbon source, which comprises the following steps: by taking fruit wastes such as apple pomace, grape pomace, banana peel and orange peel as raw materials, carrying out crushing and sterilization pretreatment, then carrying out segmented fermentation, composite enzymolysis and sodium alginate-calcium chloride crosslinking, and finally, carrying out vacuum freeze drying to obtain a powdery carbon source. The COD content of the carbon source reaches 1.5-20 thousand mg / L, the specific surface area is 100-150 m < 2 > / g, the total nitrogen removal rate is larger than or equal to 95% when the adding amount is 200 mg / L, and the turbidity reduction rate is 80-88.2%. According to the method, the integration of a high-COD carbon source and a flocculation function is realized through fruit waste recycling, segmented bioconversion and gel network construction, and the method is suitable for municipal and industrial wastewater treatment.

The invention discloses a method for preparing a biological bacterial fertilizer from coal and microorganisms, and belongs to the technical field of biological fertilizers. The method comprises the following steps: crushing coal to 80 meshes to obtain coal powder; adding a compound fertilizer accounting for 3% of the total fertilizer mass; a composite additive accounting for 1% of the total mass of the fertilizer is added, and the additive comprises 95% of mushroom sticks, 3% of glucose and 2% of microbial flora (bacillus subtilis, actinomycetes, lactic acid bacteria and the like are mixed according to the proportion of 1: 1: 1: 1); the raw materials are mixed and granulated. The organic matter content of the prepared biological bacterial fertilizer is larger than or equal to 30%, the effective viable count is larger than or equal to 2 * 10 < 7 > CFU / g, the heavy metal content is remarkably lower than the national standard, and cadmium is smaller than or equal to 0.5 mg / kg, lead is smaller than or equal to 30 mg / kg, and chromium is smaller than or equal to 80 mg / The method realizes efficient biotransformation of coal resources, and is suitable for soil improvement and organic agricultural production.

The invention relates to lactobacillus mucilaginosus and application thereof in preparation of ginsenoside F4, and belongs to the technical field of biotransformation of traditional Chinese medicinal materials. The technical problems that in the prior art, a biotransformation technology of rare ginsenoside F4 is insufficient in selectivity and environmental friendliness and high in cost are solved. The lactobacillus mucus disclosed by the invention is classified and named as LA729; the lactobacillus mucus is preserved in the China Center for Type Culture Collection (CCTCC) on August 4, 2025, and the preservation number of the lactobacillus mucus is CCTCC NO: M 20251765. The strain is safe in source and free of toxic and side effects, can secrete specific glycosidase in the fermentation process and selectively hydrolyze sugar residues on ginsenoside Re, so that structural reconstruction of the ginsenoside Re is achieved, target rare ginsenoside F4 is generated, selectivity is high, by-products are few, and the yield is stable.

The invention relates to a plant lactobacillus and application thereof in fermentation of a grape seed extract. The strain is named as FBL002, and is preserved in the Guangdong Microbial Culture Collection Center, the preservation address is the 5th floor of the Experimental Building, No.100 Court, Middle Xianlie Road, Xiaoxian District, Guangzhou City, Guangdong Province, and the preservation number is GDMCC No.66015. The tolerance of the plant lactobacillus provided by the invention to active ingredients in the grape seed extract is improved by 34%, and meanwhile, the activity of beta-glucosidase is remarkably improved to 204U / mL. In fermentation application, the strain shows excellent biotransformation performance, the content of total phenols and the content of total flavonoids in the fermented grape seed extract are increased by 45% and 98% respectively compared with an unfermented system, and meanwhile, the scavenging rate of antioxidant activity ABTS free radicals and the scavenging rate of DPPH are remarkably increased to 95%. The strain shows important application value in the fields of functional food development and natural cosmetic raw material production, and an innovative solution is provided for high-value utilization of plant resources.

The invention relates to the technical field of production of potassium fulvate, in particular to a process for producing edible alcohol and co-producing potassium fulvate by fermenting beet. The method comprises the following steps: performing coarse crushing and fine crushing on beet to form slurry, adding squeezed water, and collecting into an adjusting tank; transferring the slurry into a conversion tank for biological conversion, then carrying out solid-liquid separation treatment to obtain juice and wet residues, and squeezing the wet residues; mixing the juice with the wine mother liquor, performing two-stage fermentation to obtain fermentation liquor, and concentrating the fermentation liquor to obtain edible alcohol and waste liquor; distilling the waste liquid and adding a fulvic acid transforming agent for fermentative degradation, and then evaporating to remove moisture and concentrating to generate a concentrated solution; lime milk and the concentrated solution are uniformly mixed and stirred for neutralization, and the neutralized concentrated solution is dehydrated and dried to generate potassium fulvic acid dry powder. According to the method disclosed by the invention, polysaccharide substances in the beet are subjected to sufficient enzymolysis by utilizing the biotransformation agent, and sugar contained in the beet is sufficiently released, so that the utilization rate of beet sugar is increased.

The invention discloses a method for biologically converting isorhamnetin, which comprises the following steps: adding a microorganism for producing glucosidase, glycosyl transferase and / or succinyl transferase, or producing glucosidase, glycosyl transferase and / or succinyl transferase into an isorhamnetin substrate, adding a glycosyl donor for synchronous conversion, and separating and purifying, thereby obtaining the isorhamnetin. And the isorhamnetin-3-O-glucoside, the isorhamnetin-3-O-beta-(6 ''-O-succinyl)-D-glucoside and / or the isorhamnetin-3, 7-O-diglucoside are obtained after the reaction is finished. The method is green, safe and high in conversion efficiency, the product has higher water solubility, the bioavailability and additional value of the flavone compound can be improved, and high-valued utilization of natural plant product raw materials is achieved.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses a glucose dehydrogenase GOX-2015 mutant and application thereof, and relates to the technical field of bioengineering, the amino acid sequence of wild type glucose dehydrogenase GOX-2015 is designed and modified, that is, 18 single mutation sites and 16 combined mutation sites are provided, and the glucose dehydrogenase GOX-2015 mutant is obtained. Compared with the wild type GOX-2015, the thermal stability of the obtained GOX-2015 mutant is improved by about 2 to 13 DEG C, and the activity of the obtained GOX-2015 mutant is improved by about 1.2 to 4.5 times. The invention further provides a preferable mutant protein which has higher yield, activity and thermal stability compared with wild type GOX-2015, has wider application conditions, is more suitable for efficient production of NADPH coenzyme by a biological conversion method, and is beneficial to large-scale production and industrial application.

The invention discloses a 3D (three-dimensional) visual bionic intestine-blood vessel-nerve interface organ simulation chip, which is characterized in that a micro-fluidic chip is used as a platform to construct an intestinal tract-blood vessel-brain system which simulates biological transformation of perfluorinated compounds in intestinal tracts under near physiological conditions and biologically transports the perfluorinated compounds through blood vessels; a neuronal cell culture chamber, a vascular endothelial cell culture chamber and an intestinal tract cell culture chamber are respectively arranged on the micro-fluidic chip, the culture chambers are communicated through micro-columns, and the vascular endothelial cell culture chamber serves as a barrier in the middle; perfluorinated compounds enter corresponding cell culture cavities for culture through reagent injection channels of different cell culture channels, generated metabolites are discharged through the channels, and multi-organ interaction and toxicity induced by the perfluorinated compounds are analyzed. The chip can simulate an intestinal barrier structure, a blood vessel and a neural network structure, can highly simulate biological transport and transformation of a perfluorinated compound between intestines and brains, and effectively reduces cost and time.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The invention belongs to the field of biopharmacy and biotransformation, and particularly relates to a nicotinamide mononucleotide adenosine transferase mutant and application thereof. According to the invention, NMN is taken as a substrate, ATP disodium salt is taken as an auxiliary substrate, and NAD < + > is prepared under the catalysis of the nicotinamide mononucleotide adenosine transferase mutant under the action of magnesium ions, so that the enzymatic activity is improved, few byproducts are generated, and the reaction rate is high. The nicotinamide mononucleotide adenosine transferase mutant disclosed by the invention has better catalytic efficiency, and when NMN is taken as a substrate for catalytic reaction, the conversion rate is higher than that of an original enzyme, and the NAD < + > yield is also increased to a certain extent. The process overcomes the defects of various process steps, harsh reaction conditions, difficulty in separation and purification and the like of a chemical method for synthesizing NAD < + >, and is an efficient, low-cost and environment-friendly production mode.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention relates to an inositol dehydrogenase mutant and application of the inositol dehydrogenase mutant in synthesis of D-chiral inositol. The invention discloses an inositol dehydrogenase mutant, nucleic acid for coding the inositol dehydrogenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, and a recombinant inositol dehydrogenase mutant catalyst. The invention also discloses an application of the inositol dehydrogenase mutant or the recombinant inositol dehydrogenase mutant catalyst in catalysis of biotransformation of inositol to synthesize D-chiral inositol. Compared with wild-type inositol dehydrogenase and literature reports, the inositol dehydrogenase disclosed by the invention has the characteristics of high enzyme activity and strong substrate specificity, and has the advantages of mild reaction conditions, small coenzyme addition amount, economy, environmental protection and the like when being used for catalytically preparing D-chiral inositol. Therefore, the inositol dehydrogenase mutant disclosed by the invention has a very good application prospect in actual production of the health care medicine D-chiral inositol.

Provided are a ketoreductase mutant and a use thereof. The mutant is obtained by amino acid mutation on the basis of the amino acid sequence shown in SEQ ID NO. 2, and the mutation is (1) or (2): (1) any one of Y199A, Y199C, M196W, A146F, and A146L; (2) a combination of any one of Y199A, Y199C, M196W, A146F, and A146L and any one of N149L, N149I, I195L, and I195V. The obtained mutant having higher catalytic activity has a conversion rate increased by up to 2.4 times and a de value of more than 99.8%. The mutant has great potential in the biotransformation of steroidal compounds to prepare 24R-hydroxy-configured cholesterol.

The invention belongs to the technical field of bioengineering, and particularly relates to a phosphorylcholine cytidyltransferase mutant and application thereof. On the basis of phosphorylcholine cytidyltransferase MsCCT001, any one or more of N194D, K91E, G98S, S148A and C156V are mutated respectively, a series of phosphorylcholine cytidyltransferase mutants are obtained through screening, and the obtained mutants have the advantages that in the reaction of catalyzing phosphorylcholine and cytidine triphosphate to generate citicoline, the yield of the obtained mutants is increased, and the yield of the obtained mutants is increased. The catalytic efficiency of the compound is 1.90-2.41 times that of initial phosphorylcholine cytidyltransferase, and a biological enzyme preparation with higher catalytic capacity is provided for preparing citicoline by a biological conversion method.

A system for producing a hydrophobic chemical includes circulating biofilm bioreactors for synthesizing the hydrophobic products and continuous extraction into an organic solvent phase. Various configurations can be employed for assembling the bioreactors into a production module. Extraction operations can be conducted in one or more extraction vessels. In some cases, product and solvent are separated using membrane separation techniques, with solvent being rerouted to the extraction vessel(s). Methods for the biotransformation of feedstocks into chemical products also are described.

The invention relates to the technical field of biotechnology and food science, in particular to a preparation method of ganoderma lucidum exosomes containing medicinal and edible active ingredients, which comprises the following steps: culturing ganoderma lucidum mycelium fermentation liquor; adding the rhizoma polygonati solution into the ganoderma lucidum mycelium fermentation liquor, and carrying out biotransformation on a rhizoma polygonati powder substrate to obtain ganoderma lucidum exosome fermentation liquor; and separating, extracting and purifying the ganoderma lucidum exosome fermentation liquor to obtain the ganoderma lucidum exosome. The ganoderma lucidum exosome is extracted and purified, the medicine and food homology characteristic of the ganoderma lucidum exosome is fully utilized, and a new technical path is provided for development of functional food, health care products and medicines. According to the method, the corresponding exosome can be successfully extracted from the ganoderma lucidum culture solution, and the density of the exosome extracted after the polygonatum sibiricum is added is obviously higher than that of the exosome extracted without the polygonatum sibiricum, that is, after the polygonatum sibiricum is added, the active ingredients of the polygonatum sibiricum permeate into the original ganoderma lucidum exosome, and the ganoderma lucidum exosome has an obvious killing effect on cancer cells and shows a stronger killing effect.

The invention relates to a method for producing 1, 3-propylene glycol by fermenting recombinant Escherichia coli, which comprises the following steps: (1) a fermentation growth stage: enabling the recombinant Escherichia coli to grow in a culture medium and inducible expression of an enzyme capable of regulating the production of the 1, 3-propylene glycol; and (2) fermentation metabolism stage: cells of recombinant escherichia coli obtained by centrifuging the fermentation liquor obtained in the fermentation growth stage convert substrate glycerol into 1, 3-propylene glycol in a nitrogen-source-free culture medium. According to the method disclosed by the invention, the engineering bacteria are subjected to independent two-stage fermentation metabolism, so that the growth of the bacteria is limited and the activity of the bacteria is kept during biotransformation, and the mass conversion rate from glycerol to 1, 3-propylene glycol is remarkably improved compared with that of the existing method.