174 results about "Biotransformation" patented technology

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention discloses a fermentation preparation method of a high-COD (Chemical Oxygen Demand) composite carbon source, which comprises the following steps: by taking fruit wastes such as apple pomace, grape pomace, banana peel and orange peel as raw materials, carrying out crushing and sterilization pretreatment, then carrying out segmented fermentation, composite enzymolysis and sodium alginate-calcium chloride crosslinking, and finally, carrying out vacuum freeze drying to obtain a powdery carbon source. The COD content of the carbon source reaches 1.5-20 thousand mg / L, the specific surface area is 100-150 m < 2 > / g, the total nitrogen removal rate is larger than or equal to 95% when the adding amount is 200 mg / L, and the turbidity reduction rate is 80-88.2%. According to the method, the integration of a high-COD carbon source and a flocculation function is realized through fruit waste recycling, segmented bioconversion and gel network construction, and the method is suitable for municipal and industrial wastewater treatment.

The invention relates to lactobacillus mucilaginosus and application thereof in preparation of ginsenoside F4, and belongs to the technical field of biotransformation of traditional Chinese medicinal materials. The technical problems that in the prior art, a biotransformation technology of rare ginsenoside F4 is insufficient in selectivity and environmental friendliness and high in cost are solved. The lactobacillus mucus disclosed by the invention is classified and named as LA729; the lactobacillus mucus is preserved in the China Center for Type Culture Collection (CCTCC) on August 4, 2025, and the preservation number of the lactobacillus mucus is CCTCC NO: M 20251765. The strain is safe in source and free of toxic and side effects, can secrete specific glycosidase in the fermentation process and selectively hydrolyze sugar residues on ginsenoside Re, so that structural reconstruction of the ginsenoside Re is achieved, target rare ginsenoside F4 is generated, selectivity is high, by-products are few, and the yield is stable.

The invention relates to the technical field of production of potassium fulvate, in particular to a process for producing edible alcohol and co-producing potassium fulvate by fermenting beet. The method comprises the following steps: performing coarse crushing and fine crushing on beet to form slurry, adding squeezed water, and collecting into an adjusting tank; transferring the slurry into a conversion tank for biological conversion, then carrying out solid-liquid separation treatment to obtain juice and wet residues, and squeezing the wet residues; mixing the juice with the wine mother liquor, performing two-stage fermentation to obtain fermentation liquor, and concentrating the fermentation liquor to obtain edible alcohol and waste liquor; distilling the waste liquid and adding a fulvic acid transforming agent for fermentative degradation, and then evaporating to remove moisture and concentrating to generate a concentrated solution; lime milk and the concentrated solution are uniformly mixed and stirred for neutralization, and the neutralized concentrated solution is dehydrated and dried to generate potassium fulvic acid dry powder. According to the method disclosed by the invention, polysaccharide substances in the beet are subjected to sufficient enzymolysis by utilizing the biotransformation agent, and sugar contained in the beet is sufficiently released, so that the utilization rate of beet sugar is increased.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention belongs to the technical field of bioengineering, and particularly relates to a phosphorylcholine cytidyltransferase mutant and application thereof. On the basis of phosphorylcholine cytidyltransferase MsCCT001, any one or more of N194D, K91E, G98S, S148A and C156V are mutated respectively, a series of phosphorylcholine cytidyltransferase mutants are obtained through screening, and the obtained mutants have the advantages that in the reaction of catalyzing phosphorylcholine and cytidine triphosphate to generate citicoline, the yield of the obtained mutants is increased, and the yield of the obtained mutants is increased. The catalytic efficiency of the compound is 1.90-2.41 times that of initial phosphorylcholine cytidyltransferase, and a biological enzyme preparation with higher catalytic capacity is provided for preparing citicoline by a biological conversion method.

The invention relates to a method for producing 1, 3-propylene glycol by fermenting recombinant Escherichia coli, which comprises the following steps: (1) a fermentation growth stage: enabling the recombinant Escherichia coli to grow in a culture medium and inducible expression of an enzyme capable of regulating the production of the 1, 3-propylene glycol; and (2) fermentation metabolism stage: cells of recombinant escherichia coli obtained by centrifuging the fermentation liquor obtained in the fermentation growth stage convert substrate glycerol into 1, 3-propylene glycol in a nitrogen-source-free culture medium. According to the method disclosed by the invention, the engineering bacteria are subjected to independent two-stage fermentation metabolism, so that the growth of the bacteria is limited and the activity of the bacteria is kept during biotransformation, and the mass conversion rate from glycerol to 1, 3-propylene glycol is remarkably improved compared with that of the existing method.

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The invention provides lactobacillus reuteri GY-18 and application thereof in increasing the content of astragaloside, and belongs to the technical field of microorganisms, fermentation and natural product biotransformation. The lactobacillus reuteri GY-18 capable of improving the content of astragaloside in a fermented astragalus membranaceus product is obtained through screening, the lactobacillus reuteri GY-18 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20252796, and the lactobacillus reuteri GY-18 can be used for improving the content of astragaloside in the fermented astragalus membranaceus product. The lactobacillus reuteri GY-18 can secrete acetylxylan esterase for promoting conversion of astragaloside into astragaloside IV, has efficient specificity on conversion of astragaloside into astragaloside IV, and is high in conversion capacity; according to the method, the content of astragaloside IV is remarkably increased within 24 hours in a semi-solid state fermentation mode, and the method has good practicability.

The invention belongs to the technical field of biology and medicinal chemistry, and discloses a transaminase mutant, a method and application of the transaminase mutant in preparation of a key chiral intermediate of rotigotine, and the preparation method of the key chiral intermediate of rotigotine is a biological conversion method using the transaminase mutant as a main enzyme. The amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutating the amino acid sequence of wild type transaminase as shown in SEQ ID NO.1, the amino acid sequence of the transaminase mutant has a mutation site in the mutated amino acid sequence, and the amino acid sequence of the transaminase mutant has more than 90% of homology with the mutated amino acid sequence. When 5-methoxy-2-tetralone (a compound shown in a formula III) is catalyzed by an enzymatic reaction method to prepare a key chiral intermediate (S)-5-methoxy-1, 2, 3, 4-tetrahydronaphthalene-2-amine (a compound shown in a formula II) of rotigotine, the product is chiral purity gt, and the purity gt of the key chiral intermediate (S)-5-methoxy-1, 2, 3, 4-tetrahydronaphthalene-2-amine is gt. The yield of the biological catalysis step is gt; 95%.

The application provides an arginine mutant and application thereof in 1,4-butanediamine biosynthesis, and belongs to the field of bioengineering. Escherichia coli The mutant is obtained by site-directed mutagenesis of key site amino acid residues based on wild-type EcAdiA from strain The application improves the enzyme activity stability of arginine decarboxylase in neutral environment, improves the conversion rate and conversion speed of 1,4-butanediamine in neutral environment, and lays a foundation for high-efficiency biosynthesis of 1,4-butanediamine.

The invention relates to the technical field of waste smoke utilization, in particular to a method for preparing 2, 5-dihydroxypyridine based on biotransformation of crude nicotine, and the method comprises the following steps: S1, obtaining a genetic engineering strain for accumulating 2, 5-dihydroxypyridine by enabling an hpo gene in pseudomonas putida S16 not to be expressed; s2, culturing the genetic engineering strain obtained in the step S1 in a culture medium to obtain a thallus suspension serving as a biocatalyst; s3, enabling the thallus suspension obtained in the step S2 to be in contact with a substrate containing nicotine, and carrying out biotransformation reaction to generate the 2, 5-dihydroxypyridine. The method provided by the invention has the advantages of simpler reaction system, fewer by-products and easiness in product separation and purification when being used for preparing the 2, 5-dihydroxypyridine.

The present invention belongs to the field of microbial fermentation technology, and discloses a plant fermentation product with soothing and repairing effects, as well as its preparation method and application. The raw materials for preparing the plant fermentation product include: plant raw materials, solvents and fermentation strains; the plant raw materials include sea buckthorn fruit and licorice; the solvents include solvent A and solvent B, solvent A includes glycerol and / or ethoxydiglycol, and solvent B includes water; the fermentation strains include at least one of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum. The present invention organically combines high-pressure homogenization and specific microbial fermentation, and high-pressure homogenization is supplemented with a specific solvent, so that plant tissue cells can be fully broken, components can be fully released and dissolved, and the utilization efficiency of plant raw materials can be improved. The plant composite liquid obtained by high-pressure homogenization is directly used as the subsequent fermentation matrix. Through the biotransformation ability of microorganisms, a plant fermentation product with a high content of active substances and significant soothing and repairing effects can be prepared.

The invention discloses a walnut kernel skin active component enrichment method based on differential pressure blasting puffing and gradient biotransformation, and relates to the technical field of agricultural product deep processing and plant active component extraction, and the method comprises the following steps: S1, carrying out differential pressure blasting puffing treatment on dried walnut kernel skin, maintaining the pressure of the material at 0.8-1.5 MPa for 10-60 seconds, and then instantaneously releasing the pressure to normal pressure within 0.05-0.2 seconds, the porosity is increased, cell walls are broken, and the specific surface area is increased; s2, mixing the puffed walnut kernel skin and water according to a solid-liquid mass ratio of 1: 10-1: 30 to form slurry, adding a compound enzyme system containing cellulase, hemicellulase, pectinase, tannase and / or protease, and performing enzymolysis for 1-4 hours under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-6.0, so that cell wall components are partially hydrolyzed and bound phenols are released; and S3, sequentially carrying out first-stage fermentation and second-stage fermentation on the materials subjected to enzymolysis.

The application discloses a 17beta-hydroxysteroid dehydrogenase mutant, an engineering bacterium and application, and uses the 17beta-hydroxysteroid dehydrogenase mutant to biotransform methyl diketone as a substrate to prepare nandrolone. The 17beta-hydroxysteroid dehydrogenase is 17beta-hydroxysteroid dehydrogenase (17beta-HSDcl) from a fungus Cochliobolus lunatus, and the mutant is a single mutant in which histidine His at the 164th position is mutated into valine Val, a single mutant in which threonine Thr at the 151st position is mutated into alanine Ala, or a double mutant in which the 164th position and the 151st position are jointly mutated. The application uses pET28a as an expression plasmid, realizes heterologous expression of the 17beta-HSDcl mutant in E.coli BL21 (DE3), and through 24h whole cell transformation of the engineering bacterium, the nandrolone yield of the single mutant H164V, the single mutant T151A and the double mutant H164V / T151A is 1.27 times, 1.30 times and 1.34 times that of before mutation, respectively. The application provides a basis for industrialized production of nandrolone by microorganisms.

The invention relates to the technical field of biological medicine, and discloses a preparation method and application of a novel spironolactone derivative based on intestinal fungus biotransformation, and the method comprises the following steps: S1, screening an intestinal fungus Syncephalasetum monosporium as a transformation strain; s2, carrying out in-vitro fermentation culture on the screened strains; s3, extracting metabolites in the fermentation liquor; s4, identifying and separating the novel spirolactone derivative by combining mass spectrometry and a chromatographic separation technology; and S5, verifying the aldosterone receptor antagonistic activity of the derivative through molecular docking simulation. A novel spirolactone derivative is identified and separated through strain screening, fermentation condition optimization, multi-stage purification and mass spectrometry and chromatographic separation technologies, the enzyme activity of the strain is activated through shake culture in a culture medium to promote metabolism, the product purity is improved through inactivation, extraction and chromatographic purification, and the problems that a product is single and low in purity in a traditional method are solved.

Provided are engineered polypeptides having dehydrogenase activity that exhibit increased catalytic efficiency for nicotinamide-based unnatural cofactors relative to wild-type or parent polypeptides that encode a dehydrogenase that utilizes nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate. Also provided are cell-free and whole cell biotransformation systems for converting a substrate into a product using a redox reaction with a re-cycled unnatural cofactor that utilizes an engineered polypeptide that has dehydrogenase activity and which exhibits increased catalytic efficiency for nicotinamide-based unnatural cofactor.

The invention discloses a cyclocarya paliurus eurotium cristatum dark tea compound beverage and a preparation method thereof. The method comprises the following steps: a biotransformation extraction step: crushing herbal raw materials to obtain mixed micro-powder, and carrying out co-fermentation enzymolysis on the mixed micro-powder and activated eurotium cristatum-containing golden flower dark tea powder in water to obtain a biotransformation herbal extracting solution; a coffee extraction step: performing low-temperature extraction and concentration on coffee beans to obtain a coffee concentrated solution; homogenizing and embedding, namely mixing the extracting solution, the concentrated solution and inulin, and performing high-pressure homogenizing treatment; and a drying and forming step: performing vacuum freeze drying on the homogenized feed liquid to obtain powder. The composition comprises the biotransformed herbal component prepared by the method, a coffee component and an inulin component. Herbal raw materials are biologically converted through an extracellular enzyme system of eurotium cristatum, the dissolution rate of functional components is increased, natural bitterness removal and sweetness increase are achieved, the product flavor is improved, and the biological activity is enhanced.

The invention provides a high-activity exo-chitinase mutant and application of the high-activity exo-chitinase mutant in degradation of chitin waste, and belongs to the technical field of enzyme engineering and waste bioconversion. According to the present invention, the semi-rational engineering design is performed on the wild type exo-chitinase derived from Paenibacillus barengltzii, and a group of exo-chitinase mutants with characteristics of high activity, high stability and strong substrate binding affinity are constructed through the site-directed mutagenesis and domain fusion strategy; the exo-chitinase mutant shows excellent thermal stability and pH stability in a wide temperature and pH range, and the exo-chitinase mutant or an enzyme preparation containing the exo-chitinase mutant can be used for large-scale degradation of agricultural and marine chitin wastes. The method has a wide application prospect in the fields of sustainable feed production, agriculture and industrial biotechnology.

The invention relates to a method for preparing a cycloheptatrienone derivative, and belongs to the technical field of bioengineering. The invention provides a method for preparing a cycloheptatrienone derivative, which is based on a framework editing strategy of biological catalysis and comprises the following steps of: performing biological catalysis on a substrate and an auxiliary substrate by using a phenol derivative as a substrate and nitroalkane as an auxiliary substrate and using multi-copper oxidase and / or a microorganism for producing the multi-copper oxidase as a catalyst to obtain the cycloheptatrienone derivative. The auxiliary substrate is used as a C1 unit to be inserted into the skeleton of the aromatic ring substrate for editing, so that the cycloheptatrienone derivative is generated. According to the method, multi-copper oxidase is used as a catalyst, nitroalkane which is simple, easy to obtain, safe and highly stable is used as a C1 unit, and a phenol derivative is successfully converted into the cycloheptatrienone derivative. According to the method, a single carbon insertion mediated skeleton editing biological catalysis strategy is constructed, the strategy breaks through the limitation of a traditional carbene insertion reaction, and the method has a great application prospect in skeleton modification in the field of chemical and biological catalysis.

This invention discloses a method and apparatus for producing selenium-enriched liquid fertilizer using invasive organisms and agricultural waste. The method involves mixing the invasive organism *Alternanthera philoxeroides* and agricultural waste rice straw in a specific ratio, then using the invasive animal *Pomacea canaliculata* to biotransform the mixture. Different forms of selenium source are added to the biotransformation residue, and selenium-enriched liquid fertilizer is prepared using an aerobic liquid composting fermentation method. The composting apparatus consists of a composting bin, an automatic oxygen supply mechanism, and an intelligent temperature control mechanism, enabling fully automated management, simple operation, and no cumbersome setup. It is suitable for the efficient production of selenium-enriched liquid compost. This invention not only provides a new approach for the green production of selenium-enriched liquid compost from invasive organisms and agricultural waste, but also provides a new technology of "high-temperature composting, nutrient enrichment, and low-carbon environmental protection," realizing the resource utilization of invasive organisms and agricultural waste by converting them into selenium-enriched liquid compost, turning harm into benefit and waste into treasure.

The invention belongs to the technical field of biology, and particularly relates to a method for preparing composite pentapeptide with a lipid-lowering and blood-glucose-lowering function by utilizing an enzymolysis-fermentation-enzymolysis technology and application of the composite pentapeptide. According to the composite pentapeptide with the lipid-lowering and blood-glucose-reducing functions, the soybeans, the highland barley, the folium mori, the ginseng and the tricholoma matsutake are specifically combined, synergistic interaction can be achieved, and the lipid-lowering and blood-glucose-reducing effects are remarkably improved. In addition, the invention also provides a scheme for preparing the polypeptide by using an enzymolysis-fermentation-enzymolysis triple biotransformation technology combined process, which is beneficial to decomposing plant macromolecular substances into easily absorbed micromolecular nutritional ingredients, and simultaneously directionally extracting the five-dimensional plant composite pentapeptide with the effect of reducing blood sugar and blood fat. The biological activity and functionality of the composite pentapeptide are improved, and high-quality raw materials are provided for prevention and treatment of diabetes and hyperlipidemia. Therefore, the technical scheme of the invention has the advantages of safety, reliability, good effect and no side effect.

The invention provides a new strain for promoting growth of larvae of hermetia illucens and application of the new strain. The strain comprises a 16S rDNA (ribosomal deoxyribonucleic acid) sequence shown in SEQ ID NO: 1 or a complementary sequence of the 16S rDNA sequence or a sequence with at least 85% of nucleic acid identity compared with the sequence shown in SEQ ID NO: 1. The Tesaracoccus sp. Tess.M30 disclosed by the invention has the characteristics of being capable of decomposing starch and resisting salt. The functional bacteria are added in the breeding process of the hermetia illucens, so that growth of larvae can be promoted, and accumulation of protein and fat of the larvae is promoted. Compared with sterile water, after the Tess.M30 is added, the body length and body weight of the hermetia illucens are respectively increased by 10.82% and 48.80%, and the accumulation of protein and fat is respectively increased by 61.65% and 76.80%. By applying the technology, the biotransformation efficiency of the hermetia illucens can be improved, and industrial production of larvae of the hermetia illucens can be promoted.

The invention discloses hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres loaded with an inorganic / organic hybrid cascade catalytic system and a preparation method of the hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres. The method comprises the following steps: coupling HGPS microspheres with an organic ligand triazole through surface epoxy modification, and reacting the HGPS microspheres with a metal ion solution to obtain a metal ion-loaded microsphere skeleton; then dispersing the framework material in a buffer solution, adding an enzyme solution and an organic ligand triazole, and carrying out a one-pot reaction to obtain the HGPS-coated MAF-7-coated FDH composite microsphere which is used as an inorganic / organic hybrid cascade catalytic system to catalyze CO2 conversion to generate formic acid. According to the method, the defects that a nano material is easy to aggregate and collapse and difficult to recover are effectively overcome, and the material has high specific surface area, excellent mass transfer performance, biocompatibility and mechanical stability. The characteristics of the MAF-7 organic ligand and the controllable porous structure are beneficial to adsorption of CO2 gas, and the MAF-7 not only can be used as a nano-enzyme for replacing carbonic anhydrase to catalyze a CO2 hydration reaction, but also can be used as a carrier for immobilizing formate dehydrogenase. According to the HGPS and MAF-7 and FDH composite microspheres, the complexity of a multi-enzyme cascade reaction is simplified, the stability and robustness of enzymes are improved, a substrate channel is shortened, the overall reaction efficiency is improved, and the HGPS and MAF-7 and FDH composite microspheres have excellent popularization prospects in the fields of CO2 bioconversion and wider cascade reaction system biocatalysis.

The invention discloses a preparation method for optimizing the flavor of illicium verum wine through synergy of biotransformation and alcohol reduction, and belongs to the technical field of wine preparation. The method comprises the following steps: mixing illicium verum essential oil, a sugar donor, an immobilized enzyme preparation and a wine base, and catalyzing ethanol and the sugar donor to generate glucoside conversion reaction by using alpha-glucosidase under a control condition. The immobilized enzyme preparation is prepared by taking nano cellulose as a core carrier, coating the core carrier with hydrophilic polymer polyvinyl alcohol (PVA) to form a shielding layer, modifying chitosan, crosslinking and activating dialdehyde starch, and covalently coupling glycosidase. By utilizing the synergistic effect of enzymatic glucoside conversion and physical volatilization, the alpha-ethyl glucoside with a healthy function is generated while the alcoholic strength is reduced, and acetic acid accumulation caused by traditional oxidative alcohol reduction is avoided; meanwhile, the PVA hydrophilic shielding layer effectively blocks non-specific adsorption of the carrier to the hydrophobic star anise essential oil, so that the retention rate of flavor substances is increased, and the problems of boring flavor and rancidity of mouth feel are solved.

The invention provides a biosynthesis method of 6-hydroxy-L-tryptophan, on the basis that a novel tryptophan 6-site hydroxylase protein family enzyme is obtained through screening, a cell factory is constructed for biotransformation, and green synthesis of 6-hydroxy-L-tryptophan is achieved. The technical problems of limited types of biocatalysts, insufficient chemical synthesis region selectivity and the like in the prior art are effectively solved. The amino acid sequence of the tryptophan 6-site hydroxylase is shown as SEQ ID NO: 1. The invention provides a protein sequence for catalyzing a new family of tryptophan 6-site hydroxylase, and 6-hydroxy-L-tryptophan can be directly and efficiently synthesized through a cell factory. The method has the advantages of mild reaction conditions, environmental friendliness, high regioselectivity, good product purity, flexible process route and the like, and a brand new technical scheme is provided for industrial production of 6-hydroxy-L-tryptophan.

The application discloses a strain of Trichoderma harzianum HMS1 and application of the strain in degradation of low-grade lignite, and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 42173. The strain has heat resistance, the optimal growth temperature is 35-38 DEG C, and the highest growth temperature is 44 DEG C, so that the strain is suitable for high-temperature environment or reactor operation, and cooling energy consumption is significantly reduced. The strain can directly use untreated low-grade lignite as the only carbon source, and the final degradation rate reaches 49+ / -0.5% through solid culture by means of a plate method for 21 days, the final degradation rate reaches 50.4+ / -0.8% through liquid culture by means of a shaking flask method for 7 days, and the final degradation rate reaches 51.3+ / -1.2% through liquid culture by means of a shaking flask method for 14 days. The strain overcomes the problem that the activity of the existing mesophilic strain is significantly reduced or even inactivated in a high-temperature environment (greater than 35 DEG C), and is particularly suitable for lignite biotransformation treatment and high-temperature composting in a hot and humid area (such as Yunnan, the eastern part of Inner Mongolia, etc. in China) where lignite resources are rich.

This invention discloses a method for mine ecological restoration based on modified acidic wastewater sludge loaded with microorganisms, belonging to the field of ecological restoration technology. The method includes: S1 dewatering the acidic wastewater sludge to achieve a preset moisture content; S2 mixing the dewatered acidic wastewater sludge with a geopolymer precursor, porous carbon material, and an alkaline activator to conduct a geopolymerization reaction, followed by curing to form a structured ecological substrate; S3 inoculating modular microbial communities (including alkali-tolerant pioneer bacteria, SRB, and PGPR) into the structured ecological substrate that has been naturally matured for more than 14 days (pH ≤ 8.5) at a rate of 5–8 mL / kg dry weight (bacterial concentration ≥ 1 × 10⁻⁶). 8 (CFU / mL). This invention constructs a synergistic remediation system through a layered design, encompassing pollutant stabilization and isolation, biotransformation, and vegetation reconstruction, thereby achieving the safe resource utilization of acidic wastewater sludge and the efficient and long-term restoration of mine ecosystems.