238 results about "Biotransformation" patented technology

The invention discloses a method for efficient conversion and utilization of mogroside. According to the method, mogroside V is converted through fermentation of candida parapsilosis L7, and siamenoside I is directionally synthesized. According to the invention, Candida parapsiloides L7 is utilized for fermentation conversion, and beta-1, 6-glucosidic bonds in glucose branched chains connected with C3-site hydroxyl of a mogroside V structure parent nucleus can be directionally hydrolyzed with high regioselectivity (the beta-1, 6-glucosidic bonds on glucose branched chains connected with C24-site hydroxyl are hardly hydrolyzed), so that one molecule of glucose is removed, and siamenoside I is obtained. The siamenoside I provided by the invention can be efficiently prepared, and the maximum molar yield of the siamenoside I can reach 87.40%. The siamenoside I has more obvious selective hydrolysis characteristic, higher space time yield of the siamenoside I, no cytotoxicity of the siamenoside I and better anti-inflammatory and antioxidant activity, provides a new drug choice for diseases such as inflammation and the like, and provides technical support for development of high-valued products of mogroside.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention belongs to the field of biological pharmacy and biotransformation, and particularly relates to a tagatose-4-epimerase mutant as well as a coding gene and application thereof. The tagatose-4-epimerase mutant disclosed by the invention is obtained by carrying out single mutation or combined mutation on an amino acid residue at a specific position of an amino acid sequence as shown in SEQ ID NO.1. Compared with wild type tagatose-4-epimerase, the mutant has the advantage that the catalytic activity is improved when the D-tagatose is prepared through conversion. The obtained tagatose-4-epimerase mutant has the advantages of being mild in reaction condition, green, environmentally friendly, single in product and easy to separate and purify, and the production cost is greatly reduced.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention belongs to the technical field of microorganisms, and particularly discloses a fluoroquinolone antibiotic efficient degrading bacterium and application thereof. The fluoroquinolone antibiotic degrading bacterium is Kocuria sp. X03, the preservation number of the Kocuria sp. X03 is CCTCC NO: M 2025522, the invention provides the fluoroquinolone antibiotic degrading bacterium Kocuria sp. X03 separated from soil, the Kocuria sp. X03 can efficiently degrade fluoroquinolone antibiotics, an antibiotic degrading strain library is enriched, biotransformation mechanism research and bioremediation application are promoted, and the application prospect is wide. The strain can be used for degrading and eliminating livestock and poultry manure and residual antibiotics in farmland soil, and has certain research and application values.

The invention discloses a fermentation preparation method of a high-COD (Chemical Oxygen Demand) composite carbon source, which comprises the following steps: by taking fruit wastes such as apple pomace, grape pomace, banana peel and orange peel as raw materials, carrying out crushing and sterilization pretreatment, then carrying out segmented fermentation, composite enzymolysis and sodium alginate-calcium chloride crosslinking, and finally, carrying out vacuum freeze drying to obtain a powdery carbon source. The COD content of the carbon source reaches 1.5-20 thousand mg / L, the specific surface area is 100-150 m < 2 > / g, the total nitrogen removal rate is larger than or equal to 95% when the adding amount is 200 mg / L, and the turbidity reduction rate is 80-88.2%. According to the method, the integration of a high-COD carbon source and a flocculation function is realized through fruit waste recycling, segmented bioconversion and gel network construction, and the method is suitable for municipal and industrial wastewater treatment.

The invention discloses a method for preparing a biological bacterial fertilizer from coal and microorganisms, and belongs to the technical field of biological fertilizers. The method comprises the following steps: crushing coal to 80 meshes to obtain coal powder; adding a compound fertilizer accounting for 3% of the total fertilizer mass; a composite additive accounting for 1% of the total mass of the fertilizer is added, and the additive comprises 95% of mushroom sticks, 3% of glucose and 2% of microbial flora (bacillus subtilis, actinomycetes, lactic acid bacteria and the like are mixed according to the proportion of 1: 1: 1: 1); the raw materials are mixed and granulated. The organic matter content of the prepared biological bacterial fertilizer is larger than or equal to 30%, the effective viable count is larger than or equal to 2 * 10 < 7 > CFU / g, the heavy metal content is remarkably lower than the national standard, and cadmium is smaller than or equal to 0.5 mg / kg, lead is smaller than or equal to 30 mg / kg, and chromium is smaller than or equal to 80 mg / The method realizes efficient biotransformation of coal resources, and is suitable for soil improvement and organic agricultural production.

The invention relates to lactobacillus mucilaginosus and application thereof in preparation of ginsenoside F4, and belongs to the technical field of biotransformation of traditional Chinese medicinal materials. The technical problems that in the prior art, a biotransformation technology of rare ginsenoside F4 is insufficient in selectivity and environmental friendliness and high in cost are solved. The lactobacillus mucus disclosed by the invention is classified and named as LA729; the lactobacillus mucus is preserved in the China Center for Type Culture Collection (CCTCC) on August 4, 2025, and the preservation number of the lactobacillus mucus is CCTCC NO: M 20251765. The strain is safe in source and free of toxic and side effects, can secrete specific glycosidase in the fermentation process and selectively hydrolyze sugar residues on ginsenoside Re, so that structural reconstruction of the ginsenoside Re is achieved, target rare ginsenoside F4 is generated, selectivity is high, by-products are few, and the yield is stable.

The invention relates to a plant lactobacillus and application thereof in fermentation of a grape seed extract. The strain is named as FBL002, and is preserved in the Guangdong Microbial Culture Collection Center, the preservation address is the 5th floor of the Experimental Building, No.100 Court, Middle Xianlie Road, Xiaoxian District, Guangzhou City, Guangdong Province, and the preservation number is GDMCC No.66015. The tolerance of the plant lactobacillus provided by the invention to active ingredients in the grape seed extract is improved by 34%, and meanwhile, the activity of beta-glucosidase is remarkably improved to 204U / mL. In fermentation application, the strain shows excellent biotransformation performance, the content of total phenols and the content of total flavonoids in the fermented grape seed extract are increased by 45% and 98% respectively compared with an unfermented system, and meanwhile, the scavenging rate of antioxidant activity ABTS free radicals and the scavenging rate of DPPH are remarkably increased to 95%. The strain shows important application value in the fields of functional food development and natural cosmetic raw material production, and an innovative solution is provided for high-value utilization of plant resources.

The invention relates to the technical field of production of potassium fulvate, in particular to a process for producing edible alcohol and co-producing potassium fulvate by fermenting beet. The method comprises the following steps: performing coarse crushing and fine crushing on beet to form slurry, adding squeezed water, and collecting into an adjusting tank; transferring the slurry into a conversion tank for biological conversion, then carrying out solid-liquid separation treatment to obtain juice and wet residues, and squeezing the wet residues; mixing the juice with the wine mother liquor, performing two-stage fermentation to obtain fermentation liquor, and concentrating the fermentation liquor to obtain edible alcohol and waste liquor; distilling the waste liquid and adding a fulvic acid transforming agent for fermentative degradation, and then evaporating to remove moisture and concentrating to generate a concentrated solution; lime milk and the concentrated solution are uniformly mixed and stirred for neutralization, and the neutralized concentrated solution is dehydrated and dried to generate potassium fulvic acid dry powder. According to the method disclosed by the invention, polysaccharide substances in the beet are subjected to sufficient enzymolysis by utilizing the biotransformation agent, and sugar contained in the beet is sufficiently released, so that the utilization rate of beet sugar is increased.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses a glucose dehydrogenase GOX-2015 mutant and application thereof, and relates to the technical field of bioengineering, the amino acid sequence of wild type glucose dehydrogenase GOX-2015 is designed and modified, that is, 18 single mutation sites and 16 combined mutation sites are provided, and the glucose dehydrogenase GOX-2015 mutant is obtained. Compared with the wild type GOX-2015, the thermal stability of the obtained GOX-2015 mutant is improved by about 2 to 13 DEG C, and the activity of the obtained GOX-2015 mutant is improved by about 1.2 to 4.5 times. The invention further provides a preferable mutant protein which has higher yield, activity and thermal stability compared with wild type GOX-2015, has wider application conditions, is more suitable for efficient production of NADPH coenzyme by a biological conversion method, and is beneficial to large-scale production and industrial application.

The invention discloses a 3D (three-dimensional) visual bionic intestine-blood vessel-nerve interface organ simulation chip, which is characterized in that a micro-fluidic chip is used as a platform to construct an intestinal tract-blood vessel-brain system which simulates biological transformation of perfluorinated compounds in intestinal tracts under near physiological conditions and biologically transports the perfluorinated compounds through blood vessels; a neuronal cell culture chamber, a vascular endothelial cell culture chamber and an intestinal tract cell culture chamber are respectively arranged on the micro-fluidic chip, the culture chambers are communicated through micro-columns, and the vascular endothelial cell culture chamber serves as a barrier in the middle; perfluorinated compounds enter corresponding cell culture cavities for culture through reagent injection channels of different cell culture channels, generated metabolites are discharged through the channels, and multi-organ interaction and toxicity induced by the perfluorinated compounds are analyzed. The chip can simulate an intestinal barrier structure, a blood vessel and a neural network structure, can highly simulate biological transport and transformation of a perfluorinated compound between intestines and brains, and effectively reduces cost and time.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention relates to an inositol dehydrogenase mutant and application of the inositol dehydrogenase mutant in synthesis of D-chiral inositol. The invention discloses an inositol dehydrogenase mutant, nucleic acid for coding the inositol dehydrogenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, and a recombinant inositol dehydrogenase mutant catalyst. The invention also discloses an application of the inositol dehydrogenase mutant or the recombinant inositol dehydrogenase mutant catalyst in catalysis of biotransformation of inositol to synthesize D-chiral inositol. Compared with wild-type inositol dehydrogenase and literature reports, the inositol dehydrogenase disclosed by the invention has the characteristics of high enzyme activity and strong substrate specificity, and has the advantages of mild reaction conditions, small coenzyme addition amount, economy, environmental protection and the like when being used for catalytically preparing D-chiral inositol. Therefore, the inositol dehydrogenase mutant disclosed by the invention has a very good application prospect in actual production of the health care medicine D-chiral inositol.

The invention belongs to the technical field of bioengineering, and particularly relates to a phosphorylcholine cytidyltransferase mutant and application thereof. On the basis of phosphorylcholine cytidyltransferase MsCCT001, any one or more of N194D, K91E, G98S, S148A and C156V are mutated respectively, a series of phosphorylcholine cytidyltransferase mutants are obtained through screening, and the obtained mutants have the advantages that in the reaction of catalyzing phosphorylcholine and cytidine triphosphate to generate citicoline, the yield of the obtained mutants is increased, and the yield of the obtained mutants is increased. The catalytic efficiency of the compound is 1.90-2.41 times that of initial phosphorylcholine cytidyltransferase, and a biological enzyme preparation with higher catalytic capacity is provided for preparing citicoline by a biological conversion method.

The invention relates to the technical field of biotechnology and food science, in particular to a preparation method of ganoderma lucidum exosomes containing medicinal and edible active ingredients, which comprises the following steps: culturing ganoderma lucidum mycelium fermentation liquor; adding the rhizoma polygonati solution into the ganoderma lucidum mycelium fermentation liquor, and carrying out biotransformation on a rhizoma polygonati powder substrate to obtain ganoderma lucidum exosome fermentation liquor; and separating, extracting and purifying the ganoderma lucidum exosome fermentation liquor to obtain the ganoderma lucidum exosome. The ganoderma lucidum exosome is extracted and purified, the medicine and food homology characteristic of the ganoderma lucidum exosome is fully utilized, and a new technical path is provided for development of functional food, health care products and medicines. According to the method, the corresponding exosome can be successfully extracted from the ganoderma lucidum culture solution, and the density of the exosome extracted after the polygonatum sibiricum is added is obviously higher than that of the exosome extracted without the polygonatum sibiricum, that is, after the polygonatum sibiricum is added, the active ingredients of the polygonatum sibiricum permeate into the original ganoderma lucidum exosome, and the ganoderma lucidum exosome has an obvious killing effect on cancer cells and shows a stronger killing effect.

The invention relates to a method for producing 1, 3-propylene glycol by fermenting recombinant Escherichia coli, which comprises the following steps: (1) a fermentation growth stage: enabling the recombinant Escherichia coli to grow in a culture medium and inducible expression of an enzyme capable of regulating the production of the 1, 3-propylene glycol; and (2) fermentation metabolism stage: cells of recombinant escherichia coli obtained by centrifuging the fermentation liquor obtained in the fermentation growth stage convert substrate glycerol into 1, 3-propylene glycol in a nitrogen-source-free culture medium. According to the method disclosed by the invention, the engineering bacteria are subjected to independent two-stage fermentation metabolism, so that the growth of the bacteria is limited and the activity of the bacteria is kept during biotransformation, and the mass conversion rate from glycerol to 1, 3-propylene glycol is remarkably improved compared with that of the existing method.

The invention discloses a methanol dehydrogenase mutant with improved catalytic activity, and belongs to the technical field of enzyme directed evolution and protein engineering. The methanol dehydrogenase is subjected to random mutation by means of directed evolution. After the construction of the plasmid library is completed, the methanol dehydrogenase with higher activity is screened by using a high-throughput and simple in-vivo screening system. Two methanol dehydrogenase mutants S1 (V37A) and S2 (I58T / M292T) with significantly improved methanol catalytic activity are obtained, V37 is subjected to saturation mutation on the basis, and mutants with improved formaldehyde generation rate are further screened. The methanol dehydrogenase mutant obtained by the invention can provide a good catalytic element for bioconversion of methanol, and has a wide application prospect.

The invention discloses a collaborative simulation device and a collaborative simulation method for saline water layer sequestration and sedimentary organic matter biotransformation, which are characterized in that a gas injection module and a bacterial liquid injection module are connected with a simulation experiment box body, and supercritical carbon dioxide and bacterial liquid are injected into corresponding layers of the simulation experiment box body; the changes of the injection amount and the taking amount are determined through a controller of the monitoring module, and the changes of the dissolution sealing and mineralization sealing amount of the supercritical carbon dioxide in the salt water layer and the mineralization sealing amount and conversion sealing amount of the supercritical carbon dioxide in the mudstone layer are displayed, compared and analyzed on the monitoring module. The simulation device can simulate the interaction among supercritical carbon dioxide, salt water and rocks, and meanwhile, by controlling the change of different injection amounts and displaying corresponding change rules according to an experimental data monitoring system, the accuracy of an experimental result is greatly improved, and the experimental efficiency is improved. The technical problem that an existing traditional device cannot accurately simulate underground high temperature, high pressure and real rock-fluid interaction conditions is effectively solved.

The invention provides a nano-drug based on cfDNA, ROS and Ca < 2 + > removal and NO release promotion strategies and a preparation method and application thereof, the preparation method comprises the following steps: weighing polyethyleneimine PEI and L-arginine in deionized water to prepare a template PEI-arg; adding a calcium ion chelating agent into the template, uniformly mixing, freezing and standing; a polyethyleneimine arginine template simulates biomineralization reaction catalysis and is doped with a calcium ion chelating agent to prepare the nano-drug PEI-arg (at) MON (at) BA. The bionic silicon dioxide nano-drug is successfully prepared by adopting a one-step method, and the nano-system removes free DNA through electrostatic adsorption, eliminates ROS through redox reaction, chelates excessive Ca < 2 + > by virtue of complexation, and continuously releases NO through arginine biotransformation. Benefited from the passive targeting characteristic of the liver, the nano scavenger is enriched to the liver after intravenous injection, so that the liver microcirculation can be remarkably improved, the oxidative stress level can be reduced, the inflammatory cascade reaction can be reduced, and the tissue pathological injury can be effectively relieved.

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The invention provides lactobacillus reuteri GY-18 and application thereof in increasing the content of astragaloside, and belongs to the technical field of microorganisms, fermentation and natural product biotransformation. The lactobacillus reuteri GY-18 capable of improving the content of astragaloside in a fermented astragalus membranaceus product is obtained through screening, the lactobacillus reuteri GY-18 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20252796, and the lactobacillus reuteri GY-18 can be used for improving the content of astragaloside in the fermented astragalus membranaceus product. The lactobacillus reuteri GY-18 can secrete acetylxylan esterase for promoting conversion of astragaloside into astragaloside IV, has efficient specificity on conversion of astragaloside into astragaloside IV, and is high in conversion capacity; according to the method, the content of astragaloside IV is remarkably increased within 24 hours in a semi-solid state fermentation mode, and the method has good practicability.

The invention belongs to the technical field of biology and medicinal chemistry, and discloses a transaminase mutant, a method and application of the transaminase mutant in preparation of a key chiral intermediate of rotigotine, and the preparation method of the key chiral intermediate of rotigotine is a biological conversion method using the transaminase mutant as a main enzyme. The amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutating the amino acid sequence of wild type transaminase as shown in SEQ ID NO.1, the amino acid sequence of the transaminase mutant has a mutation site in the mutated amino acid sequence, and the amino acid sequence of the transaminase mutant has more than 90% of homology with the mutated amino acid sequence. When 5-methoxy-2-tetralone (a compound shown in a formula III) is catalyzed by an enzymatic reaction method to prepare a key chiral intermediate (S)-5-methoxy-1, 2, 3, 4-tetrahydronaphthalene-2-amine (a compound shown in a formula II) of rotigotine, the product is chiral purity gt, and the purity gt of the key chiral intermediate (S)-5-methoxy-1, 2, 3, 4-tetrahydronaphthalene-2-amine is gt. The yield of the biological catalysis step is gt; 95%.

The application provides an arginine mutant and application thereof in 1,4-butanediamine biosynthesis, and belongs to the field of bioengineering. Escherichia coli The mutant is obtained by site-directed mutagenesis of key site amino acid residues based on wild-type EcAdiA from strain The application improves the enzyme activity stability of arginine decarboxylase in neutral environment, improves the conversion rate and conversion speed of 1,4-butanediamine in neutral environment, and lays a foundation for high-efficiency biosynthesis of 1,4-butanediamine.

The invention relates to the technical field of waste smoke utilization, in particular to a method for preparing 2, 5-dihydroxypyridine based on biotransformation of crude nicotine, and the method comprises the following steps: S1, obtaining a genetic engineering strain for accumulating 2, 5-dihydroxypyridine by enabling an hpo gene in pseudomonas putida S16 not to be expressed; s2, culturing the genetic engineering strain obtained in the step S1 in a culture medium to obtain a thallus suspension serving as a biocatalyst; s3, enabling the thallus suspension obtained in the step S2 to be in contact with a substrate containing nicotine, and carrying out biotransformation reaction to generate the 2, 5-dihydroxypyridine. The method provided by the invention has the advantages of simpler reaction system, fewer by-products and easiness in product separation and purification when being used for preparing the 2, 5-dihydroxypyridine.

The invention belongs to the field of organic wastewater resourceful treatment, and particularly relates to a humic acid'energy battery '-based wastewater biological treatment method and a humic acid'energy battery'-based wastewater biological treatment device. According to the invention, humic acid and a magnetic material are coupled to prepare a recyclable magnetic humic acid composite material; a wastewater resourceful treatment method is developed on the basis of the material and comprises the following cycle steps of a charging link, a degradation treatment link and a magnetic adsorption link. A treatment device is further developed on the basis of the characteristic, and intermittent charging and recycling of the magnetic humic acid composite material are achieved on the basis of the device. Charges are obtained from an electrode by utilizing the pseudocapacitance effect of natural organic matter humic acid, and are used as an energy battery to directly charge a microbial electron transfer path, so that the limitation of traditional interspecific electron transfer of microorganisms is broken through, and the problem of low microbial electron transfer efficiency in the biotransformation process of organic matters can be fundamentally solved; and the method has creative significance.

The present invention belongs to the field of microbial fermentation technology, and discloses a plant fermentation product with soothing and repairing effects, as well as its preparation method and application. The raw materials for preparing the plant fermentation product include: plant raw materials, solvents and fermentation strains; the plant raw materials include sea buckthorn fruit and licorice; the solvents include solvent A and solvent B, solvent A includes glycerol and / or ethoxydiglycol, and solvent B includes water; the fermentation strains include at least one of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum. The present invention organically combines high-pressure homogenization and specific microbial fermentation, and high-pressure homogenization is supplemented with a specific solvent, so that plant tissue cells can be fully broken, components can be fully released and dissolved, and the utilization efficiency of plant raw materials can be improved. The plant composite liquid obtained by high-pressure homogenization is directly used as the subsequent fermentation matrix. Through the biotransformation ability of microorganisms, a plant fermentation product with a high content of active substances and significant soothing and repairing effects can be prepared.

The invention discloses a walnut kernel skin active component enrichment method based on differential pressure blasting puffing and gradient biotransformation, and relates to the technical field of agricultural product deep processing and plant active component extraction, and the method comprises the following steps: S1, carrying out differential pressure blasting puffing treatment on dried walnut kernel skin, maintaining the pressure of the material at 0.8-1.5 MPa for 10-60 seconds, and then instantaneously releasing the pressure to normal pressure within 0.05-0.2 seconds, the porosity is increased, cell walls are broken, and the specific surface area is increased; s2, mixing the puffed walnut kernel skin and water according to a solid-liquid mass ratio of 1: 10-1: 30 to form slurry, adding a compound enzyme system containing cellulase, hemicellulase, pectinase, tannase and / or protease, and performing enzymolysis for 1-4 hours under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-6.0, so that cell wall components are partially hydrolyzed and bound phenols are released; and S3, sequentially carrying out first-stage fermentation and second-stage fermentation on the materials subjected to enzymolysis.