996 results about "Biotransformation" patented technology

A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stiochiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

This is a method and apparatus for treatment of liquid media making use of at least one float positioned at the top of the liquid and at least one gas diffuser placed under the float and connected to this float by at least one brace, the diffuser is connected to a gas source by at least one flexible conduit. The gas emitted from the diffuser produces a mixture with liquid having density lower than the liquid and the float partially sinks in the liquid thus increasing the submergence of the diffuser and lowering the gas flow through the diffuser. At increased submergence, the gas flow is reduced, the mixture density increases, and the float rises. A repeatable motion up and down of the float-diffuser is established producing pulsations in the liquid. The method and apparatus can be used in a multitude of chemical, pharmaceutical, petrochemical, environmental and other industries for carrying out mass transfer, chemical and biological transformations, phase separations, thickening of suspensions, mixing, suspending of particles, washing, coagulation-flocculation, membrane filtration, filtration across particulate media, filtration across floating media, mass transfer across membrane, and other processes.

A process for the treatment of liquid waste biomass, especially liquid manure compositions, wherein the biomass is converted to a fertilizer product. The process at least includes a nitrification step including a first biological conversion stage wherein ammonium is converted to nitrite using nitritifying bacteria in an aerated reactor, and a subsequent chemical oxidation stage wherein nitrite is converted to nitrate by heating the liquid waste biomass in an aerated reactor under acidic conditions. The process is particularly suitable for treating liquid manure, because of the high ammonium nitrogen contents thereof, which render the process essentially self-regulatory. In addition a process for the treatment of liquid waste biomass wherein organic matters are converted to energy sources, referred to as biogas and green cokes, and wherein nitrogen is fixed in a fertilizer product in the form of ammonium nitrate, is provided, the process including the present nitrification process.

The present invention is a kind of tobacco perfume and its preparation process. The tobacco perfume is prepared through the following steps: 1. crushing the materials including balloon flower root 150-250 weight portions, fritillary bulb 30-80 weight portions, root of straight lady bell 150-250 weight portions, licorice 30-80 weight portions, tuckahoe 150-250 weight portions and Dangshen 70-130 weight portions, adding water and inoculating microbe strain to ferment to obtain fermented product; and 2. extracting the fermented product with water soluble solvent, concentrating the extracted liquid to obtain tobacco perfume paste. Compared with available technology, the present invention has the advantages of the microbial fermentation process, mallow flavor and obvious cigarette modifying effect of the tobacco perfume.

An additive of forage for increasing the content of peptide, the utilization rate of protein and the growth speed of animals is prepared from one or more kinds of feed protein through solid fermenting by bacillus and/or bacterium lactis and/or yeast.

The invention relates to an edible fungus compost, a production method thereof and an edible fungus culture process. The edible fungus compost disclosed by the invention consists of 40 to 90 parts of residual waste (spent mushroom substrate) left over after the harvest of edible fungi, 5 to 40 parts of cottonseed hulls, 3 to 20 parts of bran, 2 to 8 parts of corn meal, 1 to 2 parts of gypsum powder, 0 to 5 parts of quicklime, 0 to 2 parts of superphosphate and 0.05 to 0.8 parts of fungus-strengthening substance, and the pH value is 6 to 9. The invention adopts the residual waste (spent mushroom substrate) left over after the harvest of edible fungi as main material ingredient to produce the edible fungus compost. By implementing the invention, the residual waste of edible fungi cannot pollute the environment any more, and is made profitable, and the resource can be recycled; an edible fungus material resource is added, the economic burden caused by the shortage and higher prices of edible fungus materials is decreased for edible fungus farmers, the production cost of the method is reduced by more than 50 percent in comparison with the production cost of the conventional method, the yield is increased by 10 to 15 percent, the bioconversion rate reaches 80 to 120 percent, and economic income and social benefit are increased. The method is applicable to a variety of edible fungi.

The invention relates to a method for extracting, separating and purifying a functionality active component Astragaloside IV from a common used traditional Chinese medicine Astragalus mongholicus, and aims to provide a simple, safe, economic and effective method for extracting, separating and purifying the Astragaloside IV from the Astragalus mongholicus with high purity. The adopted technical scheme is as follows: taking traditional Chinese medicine Astragalus mongholicus as a raw material; and adopting a series of original and efficient technologies for extracting, separation and purifying such as homogenate extraction-mixing enzyme induction biotransformation technology, negative pressure cavitation suspension extraction technology, saponin derivant hydrolysis converting technology, liquid-liquid extraction technology, macroporous absorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and devitrification at a low temperature, recrystallization technology and the like to obtain the Astragaloside IV with high purity and yield, wherein the yield thereof can be more than 0.08% and the purity can be more than 95%. The raw material used in the invention is the common used traditional Chinese medicine Astragalus mongholicus, and has abundant resources; the production process of the Astragaloside IV is simple and practicable; the target compound has high yield and purity; and the method is suitable for the industry production and application, has high additional value and excellent market application prospective.

The invention discloses a culture material for pleurotus nebrodensis and a cultivation method of the pleurotus nebrodensis. The culture material is prepared from the following components by weight percent: 50-60% of corn cob, 7-12% of lotus receptacle, 8-12% of peanut shells, 10-15% of grape seeds, 5-15% of corn flour, 1-2% of vinasse, and 1-2% of calcium carbonate. The cultivation method comprises the steps of sterilizing the culture material, inoculating after cooling, culturing hypha for 25-35 days at 25-28 DEG C, keeping the humidity of the environment to be 75-95% with 1wt% of a water-soluble carbon nano tube solution, transferring the cultured hypha into a fruiting chamber after mediotrastum dispersing, growing sporocarp for 10-12 days at 13-18 DEG C and harvesting. The culture material and the cultivation method of the pleurotus nebrodensis is characterized in that the grape seeds contain rich multi-amino acids, vitamins and mineral substances, can increase the yield, improve the quality of the pleurotus nebrodensis and increase the biotransformation efficiency of the pleurotus nebrodensis; a water-soluble carbon nano tube has the effects of promoting the plant growth, preventing from damage by external conditions and the like, and can increase the yield, improve the quality of the pleurotus nebrodensis and shorten the growth period of the pleurotus nebrodensis hypha; besides, the grown pleurotus nebrodensis has the good quality and a large pleurotus nebrodensis body.

The invention provides a butyrylcholinesterase variant having the amino acid sequence selected from SEQ ID NOS: 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof. In addition, the invention provides a method of converting a camptothecin derivative to a topoisomerase inhibitor by contacting the camptothecin derivative with a butyrylcholinesterase variant selected from SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof, under conditions that allow conversion of a camptothecin derivative to a topoisomerase inhibitor. Further, the invention provides a method of treating cancer by administering to an individual an effective amount of a butyrylcholinesterase variant selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof, exhibiting increased capability to convert a camptothecin derivative to a topoisomerase inhibitor compared to butyrylcholinesterase.

The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

The invention relates to a biological preparation method of an intermediate of atazanavir. (S)-tertiary butyl (4-chloro-3-carbonyl-1-benzene butyl-2-yl) carbamic acid ester is taken as a substrate. The preparation method comprises the following steps of adding the substrate, a cosolvent and glucose into a reactor, stirring uniformly, weighing ketoreductase powder, glucose dehydrogenase and cofactor, dissolving in a water phase buffered solution with pH of 8-9, adding the obtained mixed solution into the reactor, stirring, controlling the temperature to be at 32DEG C-34 DEG C, and beginning reacting, wherein at the beginning of the reaction, the mass volume ratio concentration of the substrate is 0.05-0.2g/ml, and the mass ratio of the added ketoreductase to cofactor to glucose to the substrate is (0.02-0.03):(0.001-0.005):(0.8-1.2):1. Compared with the prior art, the biological preparation method realizes more environment-friendly and mild biological conversion process with high reaction efficiency, and has important application value.

The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

The invention relates to a method for generating volatile fatty acid through anaerobic fermentation after the heat-caustic pretreatment is performed to organic waste with high solid concentration, and belongs to the field of the organic solid waste resource. The invention takes municipal sewage sludge as model substrate to conduct heat-caustic pretreatment, and then anaerobic acidification sludge after being treated in a heat stress and domesticated is taken as seed sludge to perform anaerobic fermentation. The solid quality concentration of the treated municipal sewage sludge can be 3.7 to 12.0 percent, the maximal productivity of the volatile fatty acid can reach 0.23g, and the organic matter in per gram of volatile fatty acid is increased by 20.1 to 61.0 percent compared with the fermentation acid with sludge which is not be pretreated. The method transfers organic matters in the solid waste, in particular, organic matter biology which is hard to degrade, to a product with economic value; the quantitative reduction of the organic solid waste is promoted; the problem that the environmental contamination caused by the solid waste is solved to some extent; the equipment for pre-treatment and fermentation process is simple; the operation is convenient; and the method has the advantages that the cost is low, the conversion rate is high, the fermentation period is short, and the production intensity is high.

The invention relates to saccharification processing technology of jerusalem artichoke by utilizing high-quality fructose biomass as a raw material and strain selection and technology optimization for producing mannitol by taking jerusalem artichoke as carbon source through fermentation. The method comprises the following steps: 1) crushing jerusalem artichoke tuber into coarse particles, filtering after water leaching and enzymolysis for 6 hours, supernating at 42 DEG C, rotating, evaporating and concentrating to obtain saccharification jerusalem artichoke juice with high concentration of fructose; 2) establishing high performance liquid chromatography analysis and detection conditions which can synchronously analyze the content of fermentation liquor substrate (glucose and fructose) and products (mannitol); and 3) inspecting the capacity for producing lactic acid and mannitol through fermentation by seven lactic acid bacteria by utilizing saccharification jerusalem artichoke juice with different concentration of total sugar, thus determining lactic acid bacteria with high transformation rate and production intensity of fructose, and optimizing production fermentation conditions and the highest concentration of tolerant substrate. Through feed-batch fermentation, production efficiency can be improved and mannitol can be continuously produced in large scale. The method not only generates no byproduct of sorbitol, but also has low production cost, wide raw material sources, simple technology, and mature technical route and can be implemented in industrialization.

The invention provides a preparation method and an application of a discarded tobacco leaf fermentation extract. Discarded tobacco leaves are fermented with beta-glucosidase, a tobacco biotransformation liquid containing various increased small-molecular aroma substances is obtained through enzymatic curing, then amino acid and reducing sugar in certain proportion are added for a high-temperature Maillard reaction under an alkaline condition, a natural tobacco Maillard reaction liquid is obtained and subjected to pumping filtration, a filtrate and filter residues are obtained, the filtrate is concentrated and mixed with propylene glycol and the like, further separation and purification are performed through molecular distillation, and light components are collected and taken as the discarded tobacco leaf fermentation extract. Reconstituted tobacco substrates with low irritation, low miscellaneous gas, low nicotine content and low harm are prepared from the filter residues with a paper-making process, the quality of the thin discarded tobacco leaf substrates is improved, the use effect of the discarded tobacco leaves on cigarette products is improved, the adding quantity of the extract is increased, the extract can be applied to an atomization filler material for novel carbon heating tobacco, and the quality of the atomization filler material is improved.

The invention provides a method for oriented biosynthesis of GAMG, and belongs to the technical fields of biosynthesis and biotransformation. The method comprises the following steps: producing corresponding beta-D-glucuronidase by penicillium purpurogenum stoll in a fermentation medium; adding the obtained beta-D-glucuronidase into conversion solution containing glycyrrhizic acid substrate, orientedly hydrolyzing the glycyrrhizic acid under the action of enzyme, and obtaining a coarse product of GAMG; and performing organic solvent extraction, resin adsorption and purification to obtain a GAMG product. The method takes microbialenzyme as a catalyst, and has high reaction efficiency, no pollution, simple steps, and low cost; and the product is single and has high purity.

The invention relates to the new technical field of edible fungus culture, in particular to a soil covering culture method for flower mushrooms. According to the soil covering culture method, the traditional mode of directly bagging and sterilizing raw materials is changed into a mode of fermenting, bagging and sterilizing the raw materials in the flower mushroom production process, so that half time can be shortened during sterilization, and the yield of fungus bags is improved; during production and culture, bag removing and soil covering, and openness for mushroom growth are adopted, and in the management process, dry wet difference is large, and water dry-wet alternation management is carried out to stimulate the formation of the flower mushrooms, so the rate of the flower mushrooms is about 70 percent, the total biological efficiency is over 140 percent and the yield is improved by about 45 percent compared with that of the conventional culture method; and after soil covering, hyphae change color naturally, and are hardly infected with sundry fungi, and complicated management procedures of removing bags to change color, promoting mushrooms, cutting the bags to culture buttons, culturing the flower mushrooms by creating a dry environment and increasing illumination intensity and the like in the conventional method are eliminated.

The invention belongs to the field of microbial fungi, and relates to a selenium-rich cordyceps militaris strain CM001; the wild cordyceps militaris strain which is newly separated in the nature is selected as an original strain; the original strain is subjected to ultraviolet mutation, and the strain can be obtained by separating and screening; the selenium-rich cordyceps militaris strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 18, 2014 with the preservation registration number of CGMCC No.9460, is named as CM001 and is named as cordyceps militaris in a classified way. Under the artificial culture conditions, the cordyceps militaris strain has the tolerance for high-concentration selenium, and can carry out biotransformation in a body; the hyphae yeast, the growth matrix or the sporocarp of the selenium-rich cordyceps militaris strain can be used as raw materials of medicines, foods and drinks, or can be prepared into powder, tablets, freeze-drying powder, capsules or electuary.