37 results about "Xyloside" patented technology

The invention provides a method for preparing a (beta, S)-configuration glassine through enzyme catalysis and an engineering bacterium, and belongs to the field of biosynthesis, the engineering bacterium takes Escherichia coli BL21 (DE3) as an original strain, and an improved carbonyl reductase coding gene shown as SEQ ID No.1 and an improved formate dehydrogenase coding gene shown as SEQ ID No.2 are introduced. The enzyme produced by the strain is used for preparing carbonyl reductase LsADH and formate dehydrogenase PsFDHmut co-immobilized enzyme to carry out enzyme catalytic synthesis of (beta, S) configuration bose, the catalytic reaction is high in selectivity and can be carried out in a normal-temperature and normal-pressure neutral environment, the co-immobilized enzyme can be repeatedly used, and continuous production and cost reduction are facilitated. The conversion rate of the adopted catalytic reaction is greater than 99%, the used substrates beta-acetone xyloside and sodium formate are high in safety, and the synthesis efficiency of the product (beta, S) bose is remarkably improved.

The invention discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses, and belongs to the technical field of pesticide antivirus. According to the invention, targeting tobacco mosaic virus capsid protein is taken as an example, and small molecule picroside I (A0) and ZINC85593196 (B0) containing sugar rings are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. On the basis of further optimization, six compounds of 4-methylumbelliferone-beta-D-xyloside (A1), geniposide (A2), 4-methylumbelliferone acyl-beta-D-glucopyranoside (A3), 4-methylumbelliferone acyl-beta-D-glucopyranoside (A4), catalpol (A5) and aesculin (sesquihydrate) (A6) which are similar to the A0 in structure are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active component screened by the method has a good inhibition effect on plant viruses.

This invention provides a phosphorus-free, multifunctional, environmentally friendly scouring agent, its preparation method, and its application, belonging to the field of textile printing and dyeing technology. The surfactants used in this invention (including 2-ethylhexanol alkyl glycoside, 2-propylheptanol alkyl glycoside, 2-propylheptanol xyloside, and isomeric tridecyl alcohol ether carboxylic acid) are all nonionic surfactants. The resulting phosphorus-free, multifunctional, environmentally friendly scouring agent is easily soluble in water, acid-resistant, resistant to high-concentration strong alkalis, hard water-resistant, low-foaming, and has strong penetrating power. It is widely applicable to cotton and its blended textile processes, achieving excellent whiteness and wicking effect in conventional annealing / scouring / bleaching processes. It contains no alkylphenol polyoxyethylene ethers or organophosphates, causing no environmental pollution and simplifying and making textile pretreatment processes more environmentally friendly.

The present invention discloses a mutant dual-enzyme catalyst, application and (S)-Bosin synthesis method, relating to the field of bioengineering technology, the present invention is based on the methylglyoxal reductase GRE2 (A) of Candida glabrata and the phosphite dehydrogenase PTDH of methylotrophic bacteria as sources for transformation, and its mutants are screened, the key residues affecting the enzymatic properties of the initial methylglyoxal reductase and phosphite dehydrogenase are determined, and methylglyoxal reductase mutants and phosphite dehydrogenase mutants with high activity are obtained. The present invention utilizes the two most efficient mutants to catalyze the reaction of preparing (S)-Bosin from β-acetone xyloside in a 22T reaction system, with good catalytic effect, overall catalytic efficiency is increased by about 43%, and cost is reduced by about 8%.

The present invention provides a method for stereoselectively reacting a xyloside stereoisomer mixture. The method includes a step for bringing a reaction mixture that contains a cyclic esterified intermediate compound, in which a cyclic ester of boronic acid or boric acid is formed at the 2-position and the 4-position of the xylose moiety of each of α-xyloside and β-xyloside, into contact with silica gel. The step includes returning the cyclic esterified intermediate compound corresponding to α-xyloside to the α-xyloside form before cyclic esterification, and maintaining the cyclic esterified form of the cyclic esterified intermediate compound corresponding to β-xyloside.

The present invention discloses a carbonyl reductase mutant and a method for catalyzing the synthesis of high-concentration bosogenin, belonging to the field of biocatalysis. The present invention adopts a one-pot method to catalyze β-acetone xyloside to synthesize high-concentration bosogenin, uses an enzyme encoded by carbonyl reductase CnMDR, rationally designs multiple key binding sites of the substrate pocket, combines beneficial mutations, uses a recombinant bacterium with an advantageous mutant enzyme as a catalyst, uses β-acetone xyloside as a substrate, and simultaneously introduces formate dehydrogenase variants and NAD + Kinase, drives NADP + ‑NADPH coenzyme cycle, the yield of synthesized bosone is 200g / L, the conversion rate is>99%, and the product ee value is>99%, which are all the highest levels publicly reported so far. The present invention provides a one-pot method for synthesizing bosone with high efficiency and low cost, which only requires the addition of a trace amount (0.3mM) of coenzyme, and the by-products in the reaction system are easy to separate, which provides great convenience for industrial scale-up production.

The invention provides a composition with whitening and anti-aging effects as well as a preparation method and application thereof, and belongs to the technical field of medicines or cosmetics. The composition is prepared from the following components: cucurbitacine F, sutherlandin-5-trans-p-coumarate, quercetin, rutin, wogonin, trilobatin, quercetin-3-O-beta-D-xyloside, scutellarin, hyperoside, cinchona element Ib, kaempferol-3-furan arabinoside, arbutin and astragalus smicus glycoside. The preparation method comprises the following steps: preparing the cucurbitacine F, the sutherlandin-5-trans-p-coumarate, the quercetin, the rutin, the wogonin, the trilobatin, the quercetin-3-O-beta-D-xyloside and the scutellarin; the composition has the remarkable effects of whitening skin, fading spots, delaying senescence and the like, is suitable for various skin types such as dry skin types, oily skin types, mixed skin types and sensitive skin types, and has high biological application value in the fields of cosmetics and medicines.

The application discloses a kind of high stereoselectivity of alpha-xylopyranoside bond construction method, belongs to the technical field of sugar chemistry.The application uses full benzyl protected xylose trichloroacetimidate as glycosyl donor, by screening catalytic system, donor / acceptor ratio, additive type and molecular sieve type, optimize with trimethyl iodine silane as activating agent, triphenylphosphine oxide as additive high alpha-stereoselectivity glycosylation method.The method is mild in reaction condition, easy to operate, realizes the high stereoselectivity construction of 1,2-cis xylopyranoside bond without the action of participating group.Moreover, the method can be suitable for a variety of xylose donors with different protecting groups and a variety of glycosyl acceptors, to provide an effective means for efficient construction of a variety of alpha-xyloside and arabinoxylan modified by arabian mycin.

The invention provides a construction method and a detection method of a high performance liquid fingerprint of granules for consolidating foundation and clearing away lung-heat. According to the construction method, high performance liquid fingerprint spectrums of a test solution and a reference solution of the primordial qi-consolidating and lung-heat-clearing granules are compared, so that the main effective active substances of the primordial qi-consolidating and lung-heat-clearing granules are detected. The reference substance solution is prepared from 3 '-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin-6 ''-O-xyloside, puerarin apigenin, daidzin, forsythiaside A, pinoresinol-4-O-glucopyranoside, baicalin, forsythin, wogonoside, baicalein and the like. Finally, a high-performance liquid-phase fingerprint spectrum technical standard of the primordial qi-consolidating and lung-heat-clearing granules is established, the quality of the primordial qi-consolidating and lung-heat-clearing granules can be effectively and comprehensively monitored according to the characteristics of existence of common peaks in the fingerprint spectrum, and the method has the characteristics of good stability, good repeatability and strong operability.

The invention discloses a mutant of flavone-5-O-glycosyl transferase ZmDo and application of the mutant of flavone-5-O-glycosyl transferase ZmDo. Glucose donor preference key amino acid site mutation is carried out on flavone-5-O-glycosyl transferase ZmDo obtained by structural domain recombination, namely, the 142 amino acid T is mutated into V. According to the novel flavone-5-O-glycosyltransferase mutant, the glucose donor preference is obviously changed, luteolin can be specifically converted into luteolin 5-O-xyloside, compared with a wild type, the conversion rate of the generated luteolin 5-O-xyloside is reduced to 30%, the conversion rate of the generated luteolin 5-O-xyloside is increased by more than 3 times, and the novel flavone-5-O-glycosyltransferase mutant has the advantages of high yield, high yield and the like. The yield under suitable conditions can reach 4450 mg / L, and the conversion efficiency reaches 90%. The novel xylose glycosyl transferase obtained by the invention is strong in specificity and high in directional conversion efficiency, and a non-natural flavone glycoside compound luteolin 5-O-xyloside and a preparation process thereof are created.

The present invention provides a composition with whitening and anti-aging effects, a preparation method and application thereof, and belongs to the technical field of medicine or cosmetics. The composition comprises: cucurbitacin F, sutherlandin-5-trans-p-coumarate, quercetin, rutin, wogonin, trifoliol, quercetin-3-O-β-D-xyloside, scutellariae, hyperoside, cinchona sucretin Ib, kaempferol-3-furanarabinoside, arbutin and astragalus glycoside. The composition has significant whitening and anti-freckle effects, anti-aging effects, and is suitable for various skin types such as dry, oily, mixed and sensitive skin, and has high biological application value in the field of cosmetics and medicine.

The invention belongs to the technical field of microbial fermentation, and particularly relates to saccharomycetes SY and application thereof in preparation of a codonopsis pilosula fermentation product which is high in in-vivo and in-vitro bioavailability and capable of improving gastrointestinal motility. The codonopsis pilosula fermented product is prepared by fermenting codonopsis pilosula with saccharomycetes SY. The content of lobetyolin in the pilose asiabell root leavening prepared by fermenting pilose asiabell root through the saccharomycetes SY is high, in-vitro digestion experiments prove that the removal rate of lobetyolin, total flavonoids and superoxide anion free radicals and the bioaccessibility of the total reducing capacity are remarkably improved, and rat experiments find that the pilose asiabell root leavening can promote gastrointestinal motility and improve the bioavailability of the pilose asiabell root. Intestinal mucosa regeneration and barrier repair are promoted; the content of effective components such as luteolin 7-glucosid-3 '-xyloside capable of enhancing immune cell activity, regulating inflammatory factor balance, protecting cells from oxidative stress injury and improving gastrointestinal digestion and absorption functions and energy metabolism in serum is increased, and absorption of the effective components in blood is promoted.

The invention relates to the technical field of biology, in particular to a bose synthesis system and application thereof.In the presence of coenzyme NAD < + >, glucose dehydrogenase catalyzes glucose to generate gluconic acid, and NAD < + > is reduced into coenzyme NADH; in the presence of a coenzyme NADH (Nicotinamide Adenine Dinucleotide), the carbonyl reductase mutant performs carbonyl reduction catalysis on beta-acetone xyloside to generate a bose, the NADH is oxidized into NAD < + >, and the carbonyl reductase mutant generates mutation which is favorable for improving the reducibility of carbonyl in the beta-acetone xyloside at at least one key site in an amino acid sequence shown as SEQ ID NO: 40. Excellent carbonyl reduction catalytic activity is shown, so that the circulating speed of coenzymes NADH and NAD < + > is increased, and the synthesis efficiency of the bose is improved.

The invention provides a purification method and application of enzyme-catalyzed synthesized bose, and belongs to the technical field of bose purification. The purification method comprises the following steps: mixing beta-acetone xyloside, isopropanol and water, adding a compound enzyme system, carrying out an enzyme catalytic reaction, carrying out filtration, reduced pressure distillation, decoloration and filter pressing, carrying out electrodialysis, ultrafiltration, nanofiltration and vacuum concentration on the filtrate, adding absolute ethyl alcohol, crystallizing, and carrying out vacuum drying to obtain the bose, the electric field intensity of electrodialysis is 20-30 V / cm, the water flow velocity is 5-10 cm / s, and the current density is 20-30 mA / cm < 2 >. According to the method disclosed by the invention, the purity of the glassine is remarkably improved, the moisturizing effect of the glassine is proved to be better than that of a traditional process through a zebra fish experiment, and the area reduction inhibition rate is up to 72.3%. The vitreous pigment obtained by the invention can be widely applied to the field of cosmetics, and is especially suitable for anti-aging and skin collagen synthesis promoting products.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The invention relates to the field of medicine application of organic compounds, in particular to application of puerarin-6 ''-O-xyloside in preparation of medicine for treating postmenopausal lipid metabolism disorder. Animal experiments of postmenopausal lipid metabolism disorder mice induced by ovariectomy and high fat diet show that the puerarin-6 ''-O-xyloside significantly reduces the TG, TC and LDL-C levels of the mice, and also increases the HDL-C level; the liver function is improved, the ALT and AST levels are reduced, the balloon-like degeneration of liver cells and the accumulation of lipid droplets in the cells are reduced, and the volume of fat cells is reduced. From the aspect of mechanism, the PPARalpha pathway is activated to regulate and control gene expression related to fatty acid oxidation, so that liver beta-oxidation is promoted, and ectopic lipid deposition is inhibited. Therefore, the puerarin-6 ''-O-xyloside can be used for preparing a PPAR alpha agonist, a medicine for treating postmenopausal lipid metabolism disorder and a medicine for treating dyslipidemia.

The invention provides a whitening and moisturizing lotion and a preparation method thereof, and belongs to the technical field of cosmetics. The emulsion is prepared from the following components: 0.5 to 1.0 percent of sodium hyaluronate, 1 to 8 percent of glycerol, 0.1 to 2 percent of hydrogenated castor oil, 0.5 to 1.8 percent of squalane, 0.05 to 0.1 percent of polyglycerol-4 isostearate, 0.01 to 0.1 percent of behenyl glucoside, 1 to 2 percent of cetostearyl alcohol, 0.5 to 1.2 percent of octyldodecanol xyloside, 0.2 to 0.5 percent of diisostearoyl polyglycerol-3 dimer linoleate and the balance of water. The whitening and moisturizing facial mask is prepared from the following components in percentage by weight: 0.26 to 1.5 percent of a compound whitening and moisturizing factor, 0.12 to 0.68 percent of carbomer, 0.05 to 0.25 percent of beta-glucan, 0.50 to 0.78 percent of aspartic acid and the balance of deionized water. According to the invention, all the components are scientifically compounded, so that all the components have a synergistic effect, and the whitening and moisturizing effects and the overall stability of the emulsion are remarkably improved.

The invention discloses a carbonyl reductase with high stereoselectivity, a mutant and application of the carbonyl reductase in asymmetric synthesis of (S)-glass.The carbonyl reductase and the mutant can both catalyze asymmetric reduction of 50-1000 mM beta-acetone xyloside to prepare (S)-glassine, the substrate conversion rate is larger than 99%, the product is optically pure (S)-glassine, and the carbonyl reductase and the mutant can be used for preparing the (S)-glassine. The chiral purity is greater than 99%, and the method has relatively high industrial application potential.

The present invention relates to the technical field of traditional Chinese medicine quality detection methods, and specifically to a method for determining the fingerprint of a drug preparation for promoting blood circulation and removing blood stasis, promoting qi and relieving pain. The method for determining the fingerprint of the drug preparation comprises the following steps performed in sequence: extracting a test sample from a drug preparation; detecting the test sample using high performance liquid chromatography to obtain a fingerprint; comparing the fingerprint with a standard fingerprint, and then evaluating the quality of the drug preparation. The standard fingerprint includes characteristic peaks of the following components: sodium danshensu, protocatechuic aldehyde, 3'-hydroxypuerarin, puerarin, 3'-methoxypuerarin, puerarin-7-xyloside, soybean glycoside, and tannic acid B. The technical solution can solve the technical problems of the complicated operation and limited accuracy of the detection method of the prior art, and can reduce the requirements of the detection process on the equipment, reduce the detection cost, meet the conventional detection needs of pharmaceutical companies, and have a relatively ideal promotion and application value.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The base sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 95 DEG C, and after the enzyme is treated at 70 DEG C for 24 hours, the residual enzyme activity is greater than 40%. In an n-hexyl alcohol-water phase (or n-caprylic alcohol-water phase), geniposide can be hydrolyzed into genipin, and industrial production of genipin is facilitated.

The invention relates to the technical field of biology, in particular to an isopropanol system for synthesis of glassine and application thereof.In the presence of coenzyme NAD < + >, isopropanol dehydrogenase catalyzes isopropanol to generate acetone, and NAD < + > is reduced into coenzyme NADH; in the presence of a coenzyme NADH (Nicotinamide Adenine Dinucleotide), the carbonyl reductase mutant performs carbonyl reduction catalysis on beta-acetone xyloside to generate a bose, the NADH is oxidized into NAD < + >, and the carbonyl reductase mutant generates mutation which is favorable for improving the reducibility of carbonyl in the beta-acetone xyloside at at least one key site in an amino acid sequence shown as SEQ ID NO: 40. Excellent carbonyl reduction catalytic activity is shown, so that the circulating speed of coenzymes NADH and NAD < + > is increased, and the synthesis efficiency of (S)-glassine is improved.

The invention belongs to the field of chemical synthesis, and particularly relates to a synthetic method of hydroxypropyl tetrahydropyrantriol, which comprises the following steps: a) carrying out heating reaction on a ketone compound, xylose and an alkaline substance in a solvent to obtain an intermediate; and b) carrying out reduction reaction on the intermediate, an iridium reducing agent and an alkaline substance in a solvent to obtain the hydroxypropyl tetrahydropyrantriol. The synthesis method provided by the invention utilizes a Knoevenagel condensation reaction and an iridium reducing agent asymmetric reduction hydrogenation ketone carbonyl reaction, firstly, xylose and a ketone compound are used for synthesizing an intermediate (beta-xyloside), and then the iridium reducing agent is used for reducing the intermediate to obtain hydroxypropyl tetrahydropyrantriol (vitriol). The synthesis method provided by the invention does not need to use sodium borohydride as a reducing agent, does not need to use a condensing agent, does not need to remove a protecting group, and has the advantages of low cost, simple steps, high yield, high purity, convenience in post-treatment, suitability for industrialization, greenness, environmental protection and the like.

The present invention discloses an amino acid mutant of a glycosyltransferase and its application. The amino acid mutant is obtained by replacing the 141st proline of the amino acid sequence of the glycosyltransferase PhUGT derived from the Panicum hallii GT 1 family with glycine; the amino acid sequence of the amino acid mutant is shown in SEQ ID NO.3. The present invention implements directional modification of a glucosyltransferase with excellent characteristics or a certain ability to convert xylosyl groups through genetic engineering technology, and obtains a method for efficiently converting apigenin into apigenin 5-O-xyloside through a single-point mutation method. The novel amino acid mutant obtained by the present invention has strong specificity and high directional conversion efficiency, and a non-natural flavonoid glycoside compound apigenin 5-O-xyloside and a preparation process thereof are created.

The invention provides a method for preparing vitrogen through fermentation, and belongs to the technical field of biology, according to the method, an aceE gene is expressed in escherichia coli MG1655 in an enhanced mode, and an escherichia coli genetically engineered bacterium MG1655-aceE is obtained. When the gene engineering strain is used for fermentation preparation of the bose, because the expression of pyruvate dehydrogenase is enhanced, under the aerobic condition, pyruvic acid generated by glucose metabolism is promoted to react to generate acetyl-CoA, then the acetyl-CoA enters tricarboxylic acid cycle, NADH is continuously generated in the whole reaction process, and the yield of the bose is increased. The generated NADH can catalyze the synthesis reaction of a substrate beta-acetone xyloside to finally prepare the botryine, the production method provided by the invention is green and environment-friendly, the conversion rate of the botryine during shake-flask culture reaches 96.37%, and a feasible new way is provided for green production of the botryine.

The invention discloses a drug-loaded liposome complex containing a polypeptide, a preparation method and an application thereof, and belongs to the technical field of medicine. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1. The invention utilizes liposomes to encapsulate poorly soluble drugs tanshinone IIA and oroxylin B, and then compounds them with an angelica polysaccharide solution to prepare the drug complex. By encapsulating tanshinone IIA and oroxylin B with liposomes and dissolving and thickening angelica polysaccharide externally, the technical problems of poor solubility and difficulty in intra-articular injection of tanshinone IIA and oroxylin B are solved, and the obvious anti-inflammatory activity, cartilage repair and joint lubrication effects of the tanshinone IIA and oroxylin B can be exerted, and the preparation is convenient, and the tanshinone IIA and oroxylin B can be used to prepare drugs for treating or relieving inflammation and repairing cartilage, and the drug complex has a good application prospect.

The invention discloses a mutant double-enzyme catalyst, application and an (S)-vitrin synthesis method, and relates to the technical field of bioengineering.Methylglyoxal reductase GRE2 (A) of candida glabrata and phosphite dehydrogenase PTDH of methylotrophicus are used as sources for modification, mutants of the candida glabrata and the phosphite dehydrogenase PTDH are screened, and the mutant double-enzyme catalyst is obtained. The key residues influencing the enzymatic properties of the initial methylglyoxal reductase and the phosphite dehydrogenase are determined, and the methylglyoxal reductase mutant and the phosphite dehydrogenase mutant with high activity are obtained at the same time. The two mutants with the highest utilization efficiency have a good catalytic effect in a reaction for catalyzing beta-acetone xyloside to prepare (S)-bose in a 22T reaction system, the overall catalytic efficiency is improved by about 43%, and the cost is reduced by about 8%.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The amino acid sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 90 DEG C, and after the enzyme is treated at 70 DEG C for 48 hours, the residual enzyme activity is greater than 95%, so that the industrial application of preparing the blue pigment by adopting a one-pot method under a high-temperature condition is facilitated.

The invention discloses a reductase mutant and application thereof in preparation of S-vitrin. Compared with an amino acid sequence as shown in SEQ ID NO: 3, the reductase mutant has amino acid residue difference at one or more sites of Q95, L96 and G190. When the reductase mutant uses beta-acetone xyloside as a substrate to prepare the S-vitine, the conversion rate of the substrate and the ee value of the S-vitine are higher, and the reductase mutant is suitable for industrial production.

The application provides a glycosyltransferase mutant and application of the glycosyltransferase mutant in in-vitro enzymatic production of various glycoside compounds, and belongs to the technical field of novel catalytic enzymes.The application provides a glycosyltransferase VcUGT1 mutant, and the mutant can be used as a biological catalyst, can efficiently utilize various different types of glycosyl donors, and generates corresponding glycoside products, such as apigenin 7-O-glucoside, apigenin 7-O-galactoside, apigenin 7-O-N-acetylglucosamine and apigenin 7-O-xyloside.Therefore, the mutant can increase the diversity of flavonoid glycoside compounds, and has important significance for establishment of a natural medicine library and drug screening.

This invention discloses a carbonyl reductase mutant and its application in the synthesis of S-configuration Bosein, belonging to the field of biocatalytic synthesis technology. The carbonyl reductase mutant is based on the carbonyl reductase with the amino acid sequence shown in SEQ ID No. 1, including mutation types F132A, V162A, F132A-N9E, F132A-K36R, and F132A-N9E-K36R. This invention also discloses the nucleic acid molecule encoding the mutant, the expression vector, the genetically engineered strain, and a method for synthesizing S-configuration Bosein using this mutant in combination with isopropanol dehydrogenase. This carbonyl reductase mutant exhibits significantly enhanced enzyme activity and can be adapted to low-cost coenzymes. When synergistically catalyzed with isopropanol dehydrogenase, it can efficiently reduce β-pyruvate xyloside to S-configuration Bosein, with high conversion rate, short reaction time, and high product purity, significantly reducing the production cost of S-configuration Bosein and showing promising industrial application prospects.