63 results about "Xyloside" patented technology

The invention provides a fibration Cellulomonas spp and a hydrolytic enzyme thereof for effectively transforming taxane xyloside into taxol or the analogues thereof, and an effective new method for preparing the taxol or the analogues thereof through biotransformation reaction. The method can prepare the taxol or the analogues thereof with high efficiency, low cost and environmental protection, which provides an effective approach adapting to industrial production for making full use of taxane resources.

The invention discloses a method for converting astragalosides to prepare cycloastragenol by two-step enzymolysis; the method comprises the specific steps of using astragalosides as a substrate, using two different hydrolases to hydrolyze and break beta-xyloside bond at site C3 of the astragalosides and beta-glucoside bond at the site C6 respectively to obtain aglycone cycloastragenol of the astragalosides, extracting, performing silica-gel column chromatography, recrystallizing with ethanol, and purifying to obtain the finished cycloastragenol up to 95% and higher in purity. The problem that damage of three-membered ring structure of astragalosides during the preparation of cycloastragenol by a chemical process causes massive byproducts is solved, the defects of traditional cycloastragenol preparation methods, such as low substrate conversion rate, step complexity and environmental pollution, are overcome. The method has the advantages that substrate specificity is high, the substrate astragalosides is completely converted, the steps are simple, the conditions are mild, the cost is low, and the method is a mild biological preparation method, has no environmental pollution and is suitable for industrial production.

The present invention provides a UPLC method for qualitatively and quantitatively detecting the contents of six components such as 3'-hydroxy puerarin, puerarin, puerarin-6"-O-xyloside, 3'-methoxy puerarin, mirificin and daidzin in radix puerariae, radix puerariae extracts and radix puerariae-containing preparations by adopting puerarin as a reference substance. The UPLC method comprises: preparing a reference substance solution and a testing sample solution, and carrying out gradient elution by adopting 0.1% acetic acid as a mobile phase A and adopting acetonitrile as a mobile phase B, wherein the detection wavelength is 250 nm, the chromatographic column is SB-C18, RRHD 1.8 [mu]m, 2.1*100 mm, the column temperature is 31 DEG C, the flow rate is 0.4 mL / min, and the injection volume is 1 [mu]L; comparing the obtained chromatogram with a radix puerariae herb finger print, wherein the main peak is puerarin, and other components are corresponding to various peaks in a one-to-one manner so as to identify the herb; and calculating the contents of the six components in the radix puerariae by combining relative calibration factors (the relative calibration factor of 3'-hydroxy puerarin is 1.253, the relative calibration factor of puerarin is 1.000, the relative calibration factor of puerarin-6"-O-xyloside is 1.422, the relative calibration factor of 3'-methoxy puerarin is 1.297, the relative calibration factor of mirificin is 1.332, and the relative calibration factor of daidzin is 1.030) and the peak area of puerarin in the contrast solution.

Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol:(A) a protein which has the amino acid sequence of SEQ ID NO: 4;(B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

The invention belongs to the field of pharmacy, and provides application of three flavone glycoside compounds luteolin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is lonicerin), apigenin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is rhoifolin) and luteolin-6-C-beta-D-glucose-8-C-beta-D-xyloside in preparing medicament or health-care food for preventing and treating hepatitis.

The invention discloses a method for extracting taxane type active ingredients from Chinese yew efficiently. The method comprises the following steps of: firstly extracting with water, further extracting with alcohol, and further purifying by a traditional purification method to get paclitaxel, 10-deacetyl baccatin III, taxol-7-xyloside, cephalomanine and other non-water-soluble taxane type substance monomers. According to the method disclosed by the invention, macroporous resin is applied to the extraction process, the extraction yield of the active ingredients is far higher than that of a traditional extraction method, the using quantity of an organic solvent is further significantly reduced, and the method is suitable for large-scale production of the paclitaxel and comprehensive extraction of other taxane active ingredient substances.

The invention discloses a preparation method for taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) formula granules. The preparation method comprises the following steps of: after water is added into taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) for dipping, heating to 50-70 DEG C, carrying out stirring and extraction for 1.5-5 h, collecting extracting solutions, adding water into residual medicine residues for repeated extraction, and combining the extracting solutions; after the obtained extracting solution is filtered, carrying out vacuum concentration to obtain a clear cream; drying the clear cream to obtain a dry cream; and after the dry cream is smashed and sieved, obtaining a dry cream powder, then, adding auxiliary materials, evenly mixing, and carrying out granulation. While 10-deacetylation Baccatin III and 7-xyloside-10-deacetylation taxol obtained by the preparation method have a high content transfer rate, a cream yield is relatively high, and 1g of the prepared taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules isequivalent to 2.5g of taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) decoction pieces. The invention also provides a control characteristic atlas ofthe taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules, and a construction method for the control characteristic atlas, and is used for controlling the quality of the taxus chinensis (Pilg.) Rehd. var. mairei (Lemee et Levl.) Cheng et L. K. Fu (T. speciosa Florin) granules, specificity is good, and a repetitive rate is high and stable.