25 results about "Xyloside" patented technology

This invention provides a phosphorus-free, multifunctional, environmentally friendly scouring agent, its preparation method, and its application, belonging to the field of textile printing and dyeing technology. The surfactants used in this invention (including 2-ethylhexanol alkyl glycoside, 2-propylheptanol alkyl glycoside, 2-propylheptanol xyloside, and isomeric tridecyl alcohol ether carboxylic acid) are all nonionic surfactants. The resulting phosphorus-free, multifunctional, environmentally friendly scouring agent is easily soluble in water, acid-resistant, resistant to high-concentration strong alkalis, hard water-resistant, low-foaming, and has strong penetrating power. It is widely applicable to cotton and its blended textile processes, achieving excellent whiteness and wicking effect in conventional annealing / scouring / bleaching processes. It contains no alkylphenol polyoxyethylene ethers or organophosphates, causing no environmental pollution and simplifying and making textile pretreatment processes more environmentally friendly.

The present invention discloses a mutant dual-enzyme catalyst, application and (S)-Bosin synthesis method, relating to the field of bioengineering technology, the present invention is based on the methylglyoxal reductase GRE2 (A) of Candida glabrata and the phosphite dehydrogenase PTDH of methylotrophic bacteria as sources for transformation, and its mutants are screened, the key residues affecting the enzymatic properties of the initial methylglyoxal reductase and phosphite dehydrogenase are determined, and methylglyoxal reductase mutants and phosphite dehydrogenase mutants with high activity are obtained. The present invention utilizes the two most efficient mutants to catalyze the reaction of preparing (S)-Bosin from β-acetone xyloside in a 22T reaction system, with good catalytic effect, overall catalytic efficiency is increased by about 43%, and cost is reduced by about 8%.

The present invention provides a method for stereoselectively reacting a xyloside stereoisomer mixture. The method includes a step for bringing a reaction mixture that contains a cyclic esterified intermediate compound, in which a cyclic ester of boronic acid or boric acid is formed at the 2-position and the 4-position of the xylose moiety of each of α-xyloside and β-xyloside, into contact with silica gel. The step includes returning the cyclic esterified intermediate compound corresponding to α-xyloside to the α-xyloside form before cyclic esterification, and maintaining the cyclic esterified form of the cyclic esterified intermediate compound corresponding to β-xyloside.

The application discloses a kind of high stereoselectivity of alpha-xylopyranoside bond construction method, belongs to the technical field of sugar chemistry.The application uses full benzyl protected xylose trichloroacetimidate as glycosyl donor, by screening catalytic system, donor / acceptor ratio, additive type and molecular sieve type, optimize with trimethyl iodine silane as activating agent, triphenylphosphine oxide as additive high alpha-stereoselectivity glycosylation method.The method is mild in reaction condition, easy to operate, realizes the high stereoselectivity construction of 1,2-cis xylopyranoside bond without the action of participating group.Moreover, the method can be suitable for a variety of xylose donors with different protecting groups and a variety of glycosyl acceptors, to provide an effective means for efficient construction of a variety of alpha-xyloside and arabinoxylan modified by arabian mycin.

The invention discloses a mutant of flavone-5-O-glycosyl transferase ZmDo and application of the mutant of flavone-5-O-glycosyl transferase ZmDo. Glucose donor preference key amino acid site mutation is carried out on flavone-5-O-glycosyl transferase ZmDo obtained by structural domain recombination, namely, the 142 amino acid T is mutated into V. According to the novel flavone-5-O-glycosyltransferase mutant, the glucose donor preference is obviously changed, luteolin can be specifically converted into luteolin 5-O-xyloside, compared with a wild type, the conversion rate of the generated luteolin 5-O-xyloside is reduced to 30%, the conversion rate of the generated luteolin 5-O-xyloside is increased by more than 3 times, and the novel flavone-5-O-glycosyltransferase mutant has the advantages of high yield, high yield and the like. The yield under suitable conditions can reach 4450 mg / L, and the conversion efficiency reaches 90%. The novel xylose glycosyl transferase obtained by the invention is strong in specificity and high in directional conversion efficiency, and a non-natural flavone glycoside compound luteolin 5-O-xyloside and a preparation process thereof are created.

The invention belongs to the technical field of microbial fermentation, and particularly relates to saccharomycetes SY and application thereof in preparation of a codonopsis pilosula fermentation product which is high in in-vivo and in-vitro bioavailability and capable of improving gastrointestinal motility. The codonopsis pilosula fermented product is prepared by fermenting codonopsis pilosula with saccharomycetes SY. The content of lobetyolin in the pilose asiabell root leavening prepared by fermenting pilose asiabell root through the saccharomycetes SY is high, in-vitro digestion experiments prove that the removal rate of lobetyolin, total flavonoids and superoxide anion free radicals and the bioaccessibility of the total reducing capacity are remarkably improved, and rat experiments find that the pilose asiabell root leavening can promote gastrointestinal motility and improve the bioavailability of the pilose asiabell root. Intestinal mucosa regeneration and barrier repair are promoted; the content of effective components such as luteolin 7-glucosid-3 '-xyloside capable of enhancing immune cell activity, regulating inflammatory factor balance, protecting cells from oxidative stress injury and improving gastrointestinal digestion and absorption functions and energy metabolism in serum is increased, and absorption of the effective components in blood is promoted.

The invention relates to the technical field of biology, in particular to a bose synthesis system and application thereof.In the presence of coenzyme NAD < + >, glucose dehydrogenase catalyzes glucose to generate gluconic acid, and NAD < + > is reduced into coenzyme NADH; in the presence of a coenzyme NADH (Nicotinamide Adenine Dinucleotide), the carbonyl reductase mutant performs carbonyl reduction catalysis on beta-acetone xyloside to generate a bose, the NADH is oxidized into NAD < + >, and the carbonyl reductase mutant generates mutation which is favorable for improving the reducibility of carbonyl in the beta-acetone xyloside at at least one key site in an amino acid sequence shown as SEQ ID NO: 40. Excellent carbonyl reduction catalytic activity is shown, so that the circulating speed of coenzymes NADH and NAD < + > is increased, and the synthesis efficiency of the bose is improved.

The invention provides a purification method and application of enzyme-catalyzed synthesized bose, and belongs to the technical field of bose purification. The purification method comprises the following steps: mixing beta-acetone xyloside, isopropanol and water, adding a compound enzyme system, carrying out an enzyme catalytic reaction, carrying out filtration, reduced pressure distillation, decoloration and filter pressing, carrying out electrodialysis, ultrafiltration, nanofiltration and vacuum concentration on the filtrate, adding absolute ethyl alcohol, crystallizing, and carrying out vacuum drying to obtain the bose, the electric field intensity of electrodialysis is 20-30 V / cm, the water flow velocity is 5-10 cm / s, and the current density is 20-30 mA / cm < 2 >. According to the method disclosed by the invention, the purity of the glassine is remarkably improved, the moisturizing effect of the glassine is proved to be better than that of a traditional process through a zebra fish experiment, and the area reduction inhibition rate is up to 72.3%. The vitreous pigment obtained by the invention can be widely applied to the field of cosmetics, and is especially suitable for anti-aging and skin collagen synthesis promoting products.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The invention relates to the field of medicine application of organic compounds, in particular to application of puerarin-6 ''-O-xyloside in preparation of medicine for treating postmenopausal lipid metabolism disorder. Animal experiments of postmenopausal lipid metabolism disorder mice induced by ovariectomy and high fat diet show that the puerarin-6 ''-O-xyloside significantly reduces the TG, TC and LDL-C levels of the mice, and also increases the HDL-C level; the liver function is improved, the ALT and AST levels are reduced, the balloon-like degeneration of liver cells and the accumulation of lipid droplets in the cells are reduced, and the volume of fat cells is reduced. From the aspect of mechanism, the PPARalpha pathway is activated to regulate and control gene expression related to fatty acid oxidation, so that liver beta-oxidation is promoted, and ectopic lipid deposition is inhibited. Therefore, the puerarin-6 ''-O-xyloside can be used for preparing a PPAR alpha agonist, a medicine for treating postmenopausal lipid metabolism disorder and a medicine for treating dyslipidemia.

The invention provides a whitening and moisturizing lotion and a preparation method thereof, and belongs to the technical field of cosmetics. The emulsion is prepared from the following components: 0.5 to 1.0 percent of sodium hyaluronate, 1 to 8 percent of glycerol, 0.1 to 2 percent of hydrogenated castor oil, 0.5 to 1.8 percent of squalane, 0.05 to 0.1 percent of polyglycerol-4 isostearate, 0.01 to 0.1 percent of behenyl glucoside, 1 to 2 percent of cetostearyl alcohol, 0.5 to 1.2 percent of octyldodecanol xyloside, 0.2 to 0.5 percent of diisostearoyl polyglycerol-3 dimer linoleate and the balance of water. The whitening and moisturizing facial mask is prepared from the following components in percentage by weight: 0.26 to 1.5 percent of a compound whitening and moisturizing factor, 0.12 to 0.68 percent of carbomer, 0.05 to 0.25 percent of beta-glucan, 0.50 to 0.78 percent of aspartic acid and the balance of deionized water. According to the invention, all the components are scientifically compounded, so that all the components have a synergistic effect, and the whitening and moisturizing effects and the overall stability of the emulsion are remarkably improved.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The base sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 95 DEG C, and after the enzyme is treated at 70 DEG C for 24 hours, the residual enzyme activity is greater than 40%. In an n-hexyl alcohol-water phase (or n-caprylic alcohol-water phase), geniposide can be hydrolyzed into genipin, and industrial production of genipin is facilitated.

The invention relates to the technical field of biology, in particular to an isopropanol system for synthesis of glassine and application thereof.In the presence of coenzyme NAD < + >, isopropanol dehydrogenase catalyzes isopropanol to generate acetone, and NAD < + > is reduced into coenzyme NADH; in the presence of a coenzyme NADH (Nicotinamide Adenine Dinucleotide), the carbonyl reductase mutant performs carbonyl reduction catalysis on beta-acetone xyloside to generate a bose, the NADH is oxidized into NAD < + >, and the carbonyl reductase mutant generates mutation which is favorable for improving the reducibility of carbonyl in the beta-acetone xyloside at at least one key site in an amino acid sequence shown as SEQ ID NO: 40. Excellent carbonyl reduction catalytic activity is shown, so that the circulating speed of coenzymes NADH and NAD < + > is increased, and the synthesis efficiency of (S)-glassine is improved.

The invention belongs to the field of chemical synthesis, and particularly relates to a synthetic method of hydroxypropyl tetrahydropyrantriol, which comprises the following steps: a) carrying out heating reaction on a ketone compound, xylose and an alkaline substance in a solvent to obtain an intermediate; and b) carrying out reduction reaction on the intermediate, an iridium reducing agent and an alkaline substance in a solvent to obtain the hydroxypropyl tetrahydropyrantriol. The synthesis method provided by the invention utilizes a Knoevenagel condensation reaction and an iridium reducing agent asymmetric reduction hydrogenation ketone carbonyl reaction, firstly, xylose and a ketone compound are used for synthesizing an intermediate (beta-xyloside), and then the iridium reducing agent is used for reducing the intermediate to obtain hydroxypropyl tetrahydropyrantriol (vitriol). The synthesis method provided by the invention does not need to use sodium borohydride as a reducing agent, does not need to use a condensing agent, does not need to remove a protecting group, and has the advantages of low cost, simple steps, high yield, high purity, convenience in post-treatment, suitability for industrialization, greenness, environmental protection and the like.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The amino acid sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 90 DEG C, and after the enzyme is treated at 70 DEG C for 48 hours, the residual enzyme activity is greater than 95%, so that the industrial application of preparing the blue pigment by adopting a one-pot method under a high-temperature condition is facilitated.

The invention discloses a reductase mutant and application thereof in preparation of S-vitrin. Compared with an amino acid sequence as shown in SEQ ID NO: 3, the reductase mutant has amino acid residue difference at one or more sites of Q95, L96 and G190. When the reductase mutant uses beta-acetone xyloside as a substrate to prepare the S-vitine, the conversion rate of the substrate and the ee value of the S-vitine are higher, and the reductase mutant is suitable for industrial production.

The application provides a glycosyltransferase mutant and application of the glycosyltransferase mutant in in-vitro enzymatic production of various glycoside compounds, and belongs to the technical field of novel catalytic enzymes.The application provides a glycosyltransferase VcUGT1 mutant, and the mutant can be used as a biological catalyst, can efficiently utilize various different types of glycosyl donors, and generates corresponding glycoside products, such as apigenin 7-O-glucoside, apigenin 7-O-galactoside, apigenin 7-O-N-acetylglucosamine and apigenin 7-O-xyloside.Therefore, the mutant can increase the diversity of flavonoid glycoside compounds, and has important significance for establishment of a natural medicine library and drug screening.

This invention discloses a carbonyl reductase mutant and its application in the synthesis of S-configuration Bosein, belonging to the field of biocatalytic synthesis technology. The carbonyl reductase mutant is based on the carbonyl reductase with the amino acid sequence shown in SEQ ID No. 1, including mutation types F132A, V162A, F132A-N9E, F132A-K36R, and F132A-N9E-K36R. This invention also discloses the nucleic acid molecule encoding the mutant, the expression vector, the genetically engineered strain, and a method for synthesizing S-configuration Bosein using this mutant in combination with isopropanol dehydrogenase. This carbonyl reductase mutant exhibits significantly enhanced enzyme activity and can be adapted to low-cost coenzymes. When synergistically catalyzed with isopropanol dehydrogenase, it can efficiently reduce β-pyruvate xyloside to S-configuration Bosein, with high conversion rate, short reaction time, and high product purity, significantly reducing the production cost of S-configuration Bosein and showing promising industrial application prospects.

The invention relates to the technical field of resin material processing, and discloses a transparent heat-resistant PET material and a preparation method thereof.The preparation method comprises the following steps that 1, PET-1 and an additive are dissolved in a solvent system, and a first intermediate is obtained through a reaction; (2) blending, extruding and granulating the first intermediate, PET-2 (Polyethylene Terephthalate) and PETG (Polyethylene Terephthalate Glycol) to obtain a second intermediate; (3) blending, extruding and granulating the second intermediate, PAR and a chain extender to obtain a PET material; the additive is prepared from at least one of 4-methyl umbelliferone-beta-D-xyloside, 5-methoxy-9-oxo-9, 10-dihydroacridine-4-carboxylic acid and 5-hydroxy-9-oxo-9, 10-dihydroacridine-4-carboxylic acid, and the additive is prepared from at least one of 4-methyl umbelliferone-beta-D-xyloside, 5-methoxy-9-oxo-9, 10-dihydroacridine-4-carboxylic acid and 5-hydroxy-9-oxo-9 According to the method, the PET master batch is modified through the additive, the density of a benzene ring and a pyranoid ring in a molecule is increased, the rigidity of a molecular chain is further improved through the structural characteristic of 9, 10-dihydroacridine, the heat resistance of a base material is improved, the transparency of the material is improved, and meanwhile good processing performance and excellent mechanical performance are achieved.

This invention relates to the field of catalysis. It provides an application and method for a glycoside hydrolase with dual enzyme activity and its recombinant strain in the preparation of rare ginsenosides. The amino acid sequence of the glycoside hydrolase is shown in SEQ ID NO.1. Using the glycoside hydrolase of this invention or the recombinant strain expressing this enzyme, ginsenoside R1 can be catalyzed to produce rare ginsenoside Rh1, and ginsenoside Rb1 can be catalyzed to produce rare ginsenoside C-K. The glycoside hydrolase of this invention possesses both xylosidase and glucosidase activities. Therefore, using this enzyme or the recombinant strain, the xylosidic bond and glucosidic bond at the C-6 position of the aglycone of ginsenoside R1 can be simultaneously cleaved to generate rare ginsenoside Rh1. Compared with methods for preparing rare ginsenosides using glycoside hydrolases with single enzyme activity, this method has the advantages of simple process and economic efficiency, making it very suitable for large-scale industrial production.

The invention provides a phosphorus-free cotton fabric pretreatment scouring agent and application thereof, and belongs to the technical field of textile assistants. The cotton fabric pretreatment scouring agent comprises the following preparation raw materials: 10-15 parts of a nonionic surfactant, 5-10 parts of an anionic surfactant, 2 parts of a chelating agent and 73-83 parts of deionized water. Wherein the nonionic surfactant is an isomeric alcohol alkyl xyloside surfactant, and the anionic surfactant is fatty alcohol polyoxyethylene ether carboxylate. The environment-friendly alkyl xyloside and the fatty alcohol-polyoxyethylene ether carboxylate are selected as main active matters, and the scouring agent which is easy to biodegrade, high in permeability, resistant to high-concentration strong alkali and fast in defoaming is provided, so that the textile pretreatment process is simpler, more environmentally friendly, safe, non-toxic and environmentally friendly, and the textile pretreatment process is suitable for industrial production. Good use prospects are realized.

The invention relates to a chromogenic culture medium for rapid detection of mould, a test piece for rapid detection of mould and application of the chromogenic culture medium and the test piece, and belongs to the technical field of microbiological detection. The chromogenic culture medium disclosed by the invention is prepared from a basic culture component, a coagulator, 2-phenethyl alcohol, sodium butyrate, uracil, nicloramine, 5-bromo-4-chloro-3-indole-xyloside and water; the chromogenic culture medium is prepared from the following components in terms of 1L of the chromogenic culture medium: 0.2 to 15g of 2-phenethyl alcohol, 5 to 50mM of sodium butyrate, 0.015 to 0.1 mM of uracil, 0.001 to 0.08 g of nicloramine and 0.05 to 0.5 g of 5-bromo-4-chloro-3-indole-xyloside. The chromogenic culture medium is simple in formula, convenient to prepare and operate and simple to apply, can directly identify the mould in the tobacco according to the color of a growing bacterial colony during use, has the advantages of specificity, high efficiency, rapidness, accuracy, simple result judgment and the like, and has a wide application prospect.

The invention provides a method for detecting the content of multi-index components of a traditional Chinese medicine for dredging collaterals and reducing blood glucose. The detection method has the advantages of high stability, high accuracy and high reproducibility. According to the present invention, the content of the 3 '-OH puerarin is determined to be 0.0923%-0.2317%, the content of the puerarin is determined to be 0.5320%-0.6843%, the content of the puerarin-6-O-xyloside is determined to be 0.0320%-0.0412%, the content of the 3'-methoxy puerarin is determined to be 0.0972%-0.1615%, the content of the puerarin apigenin glucoside is determined to be 0.1374%-0.1712%, the content of the rutin is determined to be 0.1490%-0.3698%, and the content of the salvianolic acid is determined to be 0.3849%-0.8152%. The multi-index content determination method can also be used for the quality internal control standard of the Tongmai blood glucose reducing capsule, and the stability of the internal quality and the clinical curative effect of the product are further guaranteed.

The application discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses and belongs to the technical field of pesticide antiviral agents.Taking a tobacco mosaic virus coat protein as an example, small molecules including sugar rings, such as Swertiamarin I (A0) and ZINC85593196 (B0), are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. Based on this, further optimization is carried out, and six compounds similar to the structure of A0, such as 4-methylumbelliferone-beta-D-xyloside (A1), gardenoside (A2), 4-methylumbelliferone-beta-D-pyranoside (A3), 4-methylumbelliferone-beta-D-pyranoglucoside (A4), catalpol (A5) and esculin (hemihydrate) (A6), are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active ingredients screened by the above method have good inhibitory effect on plant viruses.

The present invention relates to a water-in-oil type nanoemulsion cosmetic composition containing an amphipathic xyloside-based emulsifier. Specifically, the present invention provides a water-in-oil type nanoemulsion cosmetic composition, which uses an emulsifier having higher affinity for water than a conventional water-in-oil type emulsifier to strongly hold an aqueous phase, thereby implementing a stable formulation and improving moisturizing capacity.