Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Discocleidion sufescens Pax et Hoffm. extract, preparation method thereof and purpose thereof

A technology for extracting and wrapping leaves, which is applied in the field of heaven and can solve problems such as separation of effective parts of Alzheimer's disease that has not been seen

Active Publication Date: 2012-06-06
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on isolating the effective part of the plant for treating Alzheimer's disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Discocleidion sufescens Pax et Hoffm. extract, preparation method thereof and purpose thereof
  • Discocleidion sufescens Pax et Hoffm. extract, preparation method thereof and purpose thereof
  • Discocleidion sufescens Pax et Hoffm. extract, preparation method thereof and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Pseudomonas herb (10.0Kg), dried, crushed and extracted with 10 times, 8 times and 8 times of 95% pharmaceutical grade ethanol respectively for 3 times, each time for 2 hours, the extract was filtered with gauze and combined Three extracts; low temperature (<80°C) to recover ethanol under reduced pressure until there is no alcohol smell to obtain ethanol extract; suspend the ethanol extract with distilled water, and use petroleum ether 5000mL×5 times, ethyl acetate 4000mL×5 times, water Saturated n-butanol 5000mL×3 extractions; the aqueous solution after the above-mentioned petroleum ether, ethyl acetate and water-saturated n-butanol extracts was concentrated under reduced pressure and low temperature (<80°C) to recover the organic solvent until there was no solvent smell, and obtained Active part extract 1.5Kg. 95% ethanol extract, petroleum ether extract, ethyl acetate extract, n-butanol extract, and active site extract were respectively used for nerve cell activity s...

Embodiment 2

[0018] Example 2: Structural identification of the active site

[0019] 100g of the active part of the pseudo-capsule, mix the sample with silica gel (0.063-0.200mm, 250g), put it on a silica gel column (0.060-0.200mm) for chromatographic separation, and dichloromethane-methanol-water (100:0:0-100 : 30:10) gradient elution, each 200mL as a fraction, a total of 100 fractions; dichloromethane-methanol-water (100:10:2) fraction through silica gel column (0.040-0.063mm), ODS and Sephadex LH- 20 column chromatographic separation to obtain compounds Ⅰ (25mg), Ⅱ (30mg); dichloromethane-methanol-water (100:10:100) was separated by silica gel column (0.040-0.063mm) and ODS column chromatography, and separated Compound Ⅲ (30 mg) was obtained; dichloromethane-methanol-water (100:20:5) part was separated by silica gel (0.040-0.063mm) and ODS repeated column chromatography to obtain compound Ⅳ (25 mg), Ⅴ (30 mg) , VI (25mg).

[0020] The structures of compound Ⅰ~compound Ⅵ were identif...

Embodiment 3

[0025] Example 3: Preliminary research data on the anti-senile dementia effect of the active site

[0026] Take 95% ethanol extract, petroleum ether extract, ethyl acetate extract, n-butanol extract, and active site extract respectively for nerve cell activity screening. The results show that 95% ethanol extract and active site extract are all Has a strong NGF-inducing activity.

[0027] 1. Principle and method

[0028] Using the nerve cell in vitro model test, using PC-12 cells, to detect the NGF-inducing activity of each part.

[0029] (1) PC 12 cells are cell lines differentiated from rat adrenal medulla pheochromocytoma, which have general characteristics of neuroendocrine cells, and are now widely used in neurophysiological and neuropharmacological research.

[0030] When cultured with very low concentration of NGF, PC 12 cells can be induced to differentiate into NGF-dependent dopaminergic neurons.

[0031] (2) LDH (Lactic dehydrogenase, lactate dehydrogenase) is us...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Discocleidion sufescens Pax et Hoffm. extract, a preparation method thereof and a purpose thereof. The extract comprises the following six ellagic acid compounds: ellagic acid (I), 3,3',4'-trimethyl ellagic acid (II), 3,3'-dimethyl ellagic acid (III), 3,3',4-trimethyl ellagic acid-4'-O-beta-D-glucoside (IV), 3,3'-dimethyl ellagic acid-4'-O-beta-D-glucoside (V), and 3,3'-dimethyl ellagic acid-4'-O-beta-D-xyloside (VI). The extract can be applied to prepare senile dementia resisting medicines.

Description

technical field [0001] The present invention relates to the technical field of natural medicine chemistry, in particular to a kind of pseudopodium from Euphorbiaceae Pseudopophyllum ( Discocleidion rufescens (Franch.) Pax et Hoffm.- Alchornea rufescens Franch.) The active part extract obtained from the whole herb, the preparation process and its application. [0002] Background technique [0003] Based on the research of natural products, combined with activity screening, the discovery of active lead compounds from Chinese herbal medicines is the source of innovative drug development. [0004] False bag leaves ( Discocleidion rufescens (Franch.) Pax et Hoffm.- Alchornea rufescens Franch.) is Euphorbiaceae (Euphorbiaceae L.). Discocleidion (Muell.-Arg.) Pax et Hoffm.) plants. There are three species of Pseudomonas, which are distributed in my country and the Ryukyu Islands. In my country, there are Discocleidion rufescens (Franch.) Pax et Hoffm. A kind, produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/366A61P25/28
Inventor 朱华旭段金廒张启春唐于平闵知大
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com