Method for screening traditional Chinese medicine effect related ingredients and model building method

A technology of medicinal efficacy and traditional Chinese medicine, which is applied in the field of screening medicinal efficacy-related components of traditional Chinese medicines, can solve the problems of high cost, poor versatility, and long time period of medicinal efficacy-related components, and achieves the effects of reliable versatility and simple method.

Active Publication Date: 2013-01-16
SHANDONG UNIV
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Problems solved by technology

[0020] In view of the above-mentioned prior art, in order to solve the problems of long time period, high cost, poor versatility and unreliable reliability of existing screening of traditional Chinese medicine efficacy-related components, the present invention provides a fast, simple, accurate and universal A better method for screening the efficacy-related components of traditional Chinese medicine, which can effectively determine the efficacy-related components, and can reflect the characteristics of the comprehensive effect of multiple components on the efficacy indicators

Method used

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  • Method for screening traditional Chinese medicine effect related ingredients and model building method
  • Method for screening traditional Chinese medicine effect related ingredients and model building method
  • Method for screening traditional Chinese medicine effect related ingredients and model building method

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Embodiment 1

[0063] The traditional Chinese medicine used in Example 1: Radix Astragali; the efficacy index used: the activity of scavenging DPPH free radicals (DPPH free radical scavenging rate). Although the traditional Chinese medicine Radix Astragali and the scavenging activity of DPPH free radicals are taken as examples, the method of the present invention has no limitation on the traditional Chinese medicines used and the efficacy indexes.

[0064] 1.1 Preparation of reference solution

[0065] Accurately weigh about 5 mg, 5 mg, 2 mg and 2 mg of the standard substances of the four chemical components kaempferol, quercetin, calycosin and formononetin contained in the medicinal material of Astragalus membranaceus, add methanol to dissolve and dilute to 10 mL brown In the volumetric flask, the stock solution of each component is obtained; accurately weigh about 10 mg of the standard product of astragaloside IV, add methanol to dissolve and set the volume in a 5mL brown volumetric flask,...

Embodiment 2

[0150] Correlation Analysis of HPLC Fingerprint Spectrum of Example 2 Radix Astragalus Medicinal Material and Antioxidative Effect

[0151] Free radicals are chemical groups with lone pairs of electrons, and are very active in nature. Current research believes that aging, certain chronic diseases, and cardiovascular diseases are closely related to them. Astragalus is a leguminous plant Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. The dried root of the root has the effects of invigorating qi and solidifying the surface, draining pus and detoxifying, astringing sores and promoting granulation, and is a commonly used Chinese medicinal material. The main components of Astragalus include flavonoids, saponins, polysaccharides, etc. Modern pharmacological studies have proved that Astragalus may have antioxidant activity. There are many methods for the determination of free radical scavenging activity, including hydrocarbo...

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Abstract

The invention discloses a method for screening traditional Chinese medicine effect related ingredients and a model building method. The method comprises the steps as follows: taking the researched traditional Chinese medicines as objects for preparing multiple sets of sample solutions containing chemical components with different concentrations; selecting part of the chemical components for HPLC (High Performance Liquid Chromatography) analysis, and investigating linear relations between the peak areas and the concentrations; selecting the multiple sets of sample solutions in the linear range, and determining the respective HPLC fingerprint spectrums and the medicine effect indexes of the sample solutions; and obtaining relations between the peak areas and the medicine effect indexes of the ingredients by adopting a linear regression analysis method in the linear relation range, and confirming the actions of the medicine effect related ingredients on the medicine effect indexes according to the positive value and the negative value of the standard regression coefficients and/or the t check results of the ingredients so as to screen out the traditional Chinese medicine effect related ingredients. The method is simple, convenient, easy to implement, reliable and good in commonality, can embody the characteristics of comprehensive actions of the multiple ingredients on the medicine effect indexes, is suitable for screening the traditional Chinese medicine effect related ingredients, and provides a technical data support for discovering and screening traditional Chinese medicine active ingredients.

Description

technical field [0001] The invention relates to a method for screening components related to efficacy of traditional Chinese medicine and a method for establishing a model. Background technique [0002] Traditional Chinese medicine is an important material basis for the clinical prevention and treatment of diseases in traditional Chinese medicine. It has the characteristics of multi-component, multi-target and multi-path. However, the composition of traditional Chinese medicine is complex, and excavating and interpreting the active ingredients of traditional Chinese medicine is the basis for the development of safe, effective and quality-controllable modern Chinese medicine. [0003] For a long time, the mainstream mode of research on the active ingredients of traditional Chinese medicine mainly draws on the basic strategies of foreign countries to study the active ingredients of natural products. [1-3] . Such a research model has played a positive role in the elucidation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 聂磊邓书鸿姜红宋丽段小菊张丹潞魏敏
Owner SHANDONG UNIV
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