87 results about "Kaempferol" patented technology

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention discloses an on-line extraction self-optimization method for acanthopanax sessiliflorus flavone based on reinforcement learning. According to the method, multi-source data in the extraction process is collected in real time, and a fusion feature vector is generated after preprocessing; establishing a mapping relation from the feature vector to the contents of rutin, quercetin, kaempferol and hyperoside by using an offline test sample, and constructing a virtual measurer capable of outputting a target flavone composition vector and uncertainty thereof; constructing a safety domain boundary according to a solvent flash point temperature, an equipment tolerance temperature upper limit and a component thermal degradation temperature threshold, and calculating a time-varying safety constraint threshold in combination with uncertainty; performing linkage optimization on the temperature, the ethanol volume fraction, the solid-to-liquid ratio, the soaking time and the circulating flow by adopting a reinforcement learning strategy, and comprehensively considering flavone composition deviation and solvent energy consumption by a reward function; and finally, outputting the optimized parameters to extraction equipment for execution. According to the method, online, lossless and self-adaptive process optimization control is realized, inter-batch fluctuation is remarkably reduced, and the resource utilization efficiency is improved.

The application relates to kaempferol cerium nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines. The kaempferol cerium nanoparticles are prepared by performing coordination co-assembly on kaempferol and cerium ions in the presence of polyethylene glycol at a specific molar ratio, and the nanoparticles have uniform particle sizes and good dispersity. The nanoparticles have the anti-inflammatory activity of kaempferol and the ROS scavenging capacity of cerium ions, and show a significant synergistic effect in in-vivo and in-vitro experiments, can effectively reduce lung inflammation, inhibit oxidative stress, improve lung function, and have good biological safety, thereby providing a new drug candidate scheme for the treatment of ARDS.

The invention discloses a copper ion-kaempferol nano-composite and application thereof in treatment of inflammatory bowel disease, the obtained nano-composite has very strong stability and active oxygen scavenging ability, and has excellent oxidation resistance and anti-inflammatory ability, and the specific performance is as follows: the surface is negatively charged, and the nano-composite can specifically target the diseased region of intestinal inflammation, so that the copper ion-kaempferol nano-composite can be applied to treatment of inflammatory bowel disease. The intestinal mucosal barrier injury is effectively repaired, the imbalance state of intestinal flora is adjusted, and the intestinal inflammation microenvironment is remarkably improved; the antioxidant and anti-inflammatory capacities of cells can be enhanced by regulating and controlling a PI3K-Akt signal channel and a nuclear factor kappa B (NF-kappa B) signal channel; the compound shows an excellent treatment effect in mouse models of typical inflammatory bowel diseases such as ulcerative colitis and Crohn's disease and related complications of bowel diseases. The invention provides a new way for constructing a multi-target and multi-layer treatment platform for the inflammatory bowel disease of a natural product.

The application belongs to the technical field of chemical analysis and detection, and discloses a zinc oxide quantum dot fluorescent sensor and application of the zinc oxide quantum dot fluorescent sensor in detection of kaempferol. The zinc oxide quantum dot fluorescent sensor is prepared by stirring zinc salt solution and lye uniformly, then adding amino silane and water to carry out sol-gel reaction to prepare a ZnO-QDs fluorescent probe solution; then, a substrate is immersed in the ZnO-QDs fluorescent probe solution to carry out incubation, and drying is carried out to obtain the zinc oxide quantum dot fluorescent sensor. S The zinc oxide quantum dot fluorescent sensor is applied to detection of kaempferol, can produce specific response with kaempferol, makes the fluorescence intensity of the ZnO-QD fluorescent probe gradually increase with the increase of the concentration of kaempferol, and can effectively exclude the interference of structural analogs such as quercetin and luteolin, so that the kaempferol in the sample can be accurately quantified; and the detection cost is low, the operation is convenient, the detection efficiency is high, the detection is green and safe, and the zinc oxide quantum dot fluorescent sensor is suitable for popularization and application.

The invention relates to the technical field of hair supplies, in particular to plant-extracted anti-hair-loss hair-growing liquid and a preparation method thereof. The plant extraction anti-hair loss hair growth liquid provided by the invention is prepared from hair growth factors, peppermint oil, isohexylene glycol, 1, 3-butanediol and water, the hair growth factor is obtained by extracting polygonum multiflorum, eclipta, white mulberry root-bark, rhizoma kaempferiae and fructus chebulae. The hair growth liquid obtained by the invention has the advantages of low irritation, moistening and transdermal effects, good compliance effect for a user and efficient hair loss prevention and hair growth effects, and hair growth active factors can be efficiently delivered and effectively play the hair loss prevention and hair growth effects.

The present application relates to the technical field of health food, and particularly relates to a composition and application thereof in maintaining blood glucose health level. The composition comprises rutin, quercetin, kaempferol and pectin. Experiments show that the composition of the present application significantly reduces the activity of alpha-glucosidase, significantly delays starch digestibility, thereby reduces the postprandial blood glucose level of mice, and the effect is obviously better than that of other flavonoid and pectin compositions. It is shown that the rutin, quercetin, kaempferol and pectin are compounded according to a certain proportion, and obvious synergistic effect is produced. In addition, the composition prepared by the present application reduces the use amount of quercetin, increases the utilization rate of pectin, reduces the production cost, and the raw materials are pure natural, safe and non-toxic, functional, low in cost, and have wide application prospect in the deep processing of starch-based health food.

This invention provides an extract of *Hedyotis diffusa*, its preparation method, and its uses. When the extract is measured under reversed-phase high-performance liquid chromatography, the peak area A of 6''-malonyl *Hedyotis diffusa* I is... M Peak area A with cypermethrin I B The ratio is less than 0.1, and the peak area A of free kaempferol is... K With A B The ratio shall not exceed 0.15. The preparation method includes: adjusting the pH of the aqueous extract of *Kaempferia galanga* to 11-13.5, and performing alkaline hydrolysis at 25-65°C for 20-60 minutes. This invention utilizes the difference in stability of malonyl bonds and glycosidic bonds under alkaline conditions to achieve the selective conversion of 6''-malonylkaempferol I to kaempferol I, increasing the content of kaempferol I while maintaining the integrity of the glycosidic bonds and inhibiting or controlling the formation of free kaempferol. The resulting extract can be used to prepare anti-inflammatory and antioxidant drugs or health foods.

The present invention relates to a use of a moiety selected from the group consisting of: (a) a nucleic acid encoding shRNA or siRNA specific for NR4A1 comprising the sequence of SEQ ID NO: 5 or SEQ ID NO: 6; (b) a NR4A1 antagonist selected from the group consisting of 1,1- bis(3'-indolyl)-1-(p-hydroxyphenyl)methane (DIM-C-pPhOH), resveratrol, a 3-chloro-5- methoxy analog (DIM-C-pPhOH-3-Cl-5-OCH3) and a flavonoid selected from the group consisting of kaempferol and quercetin; (c) a vector comprising a nucleic acid molecule encoding menin; and (d) a NF-ĸB inhibitor, in the manufacture of a medicament for treating pancreatic neuroendocrine tumors (PNETs) in a subject, wherein the medicament is to be administered to the subject, thereby reducing cellular proliferation of PNETs. The present invention also relates to a method of treating pancreatic neuroendocrine tumors (PNETs) in a subject.

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.

The invention relates to application of kaempferol in preparation of drugs, food or health care products for preventing or treating liver cancer related to non-alcoholic fatty liver or non-alcoholic fatty liver. According to the kaempferol disclosed by the invention, the liver weight, the liver weight ratio, the tumor quantity and the maximum diameter of the tumor of a mouse with the non-alcoholic fatty liver-related liver cancer are obviously reduced; in histology, the kaempferol can obviously improve hepatic tissue fatty degeneration and liver lipid droplet density, and improve liver cell inflammation and fibrosis degree; kaempferol can obviously reduce the content of cholesterol serum and cholesterol in the liver of a mouse with non-alcoholic fatty liver-related liver cancer, and can reduce liver injury indexes ALT and AST; the kaempferol can obviously reduce the expression level of mRNA related to liver fibrosis of mice with liver cancer related to non-alcoholic fatty liver.

The invention discloses a culture solution for improving in-vitro culture blastocyst rate of bovine oocytes and application of the culture solution, and belongs to the technical field of cell in-vitro culture. According to the culture solution, kaempferol is added into a basic culture solution, and the addition concentration of the kaempferol is 0.01-1 mu M / L; preferably, the addition concentration of the kaempferol is 0.1 mu M / L. By optimizing the concentration of the kaempferol and the synergistic proportion of the kaempferol and basic nutritional ingredients, the action mechanism of the kaempferol for regulating and controlling the mitochondrial function of the oocyte and meiosis spindle assembly is defined, and the direct association of'oxidation resistance-development potential improvement 'is established, so that the blastocyst formation rate and embryo quality of in-vitro culture of the oocyte are remarkably improved, and the development potential of the oocyte is improved. The requirements of the fields of assisted reproduction, embryo engineering and the like on an efficient and stable oocyte in-vitro culture technology are met.

The present application relates to the technical field of skin mucosa repair, and particularly relates to a pharmaceutical preparation for repairing skin mucosa injury. The preparation takes kaempferol, quercetin, 3-p-coumaroyl-1, 4, 6'-triacetyl sucrose, 3-p-coumaroyl-2', 3', 6'-triacetyl sucrose, (+)-syringaresinol and vitamin B12 extracted from Prunus tomentosa as active ingredients, so as to solve the problems of poor curative effect, long repair period and easy scarring of the existing skin mucosa injury repair preparation, and realize efficient, safe and high-quality skin mucosa injury repair effect.

The application relates to the field of biological medicine, and particularly relates to application of kaempferol-3-O-acacia di-saccharide-7-O-rhamnose glycoside in preparation of a medicine for preventing and / or treating diseases related to glycolipid metabolism disorder. The compound or a pharmaceutically acceptable salt, ester, solvate or prodrug thereof can be used for treating diseases such as type 2 diabetes, insulin resistance, hyperglycemia, hyperlipidemia and non-alcoholic fatty liver disease. The compound can significantly reduce intracellular glucose, triglyceride and total cholesterol levels, and can play a role by activating an AKT signal pathway, so as to improve glycolipid metabolism disorder and has potential clinical application value.

The application discloses a hypoglycemic and hypolipidemic composition from the leaves of pandamus, a preparation method and application thereof. The composition contains p-coumaric acid, rutin, kaempferol-3-O-rutinoside, pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2. The composition with a preferred mass ratio has a certain synergistic effect. Under the premise of a consistent total dose, the composition is superior to p-coumaric acid, rutin and kaempferol-3-O-rutinoside in improving insulin resistance and reducing fat deposition, and is also superior to a pandamus alkaloid composition composed of pandamarilactonine-A, pandamarilactone-1 and pandamarilactone-2, which indicates that there is a synergistic effect among the components of the composition.

This invention relates to the field of glyphosate detection, and discloses a kaempferol-Al method for detecting glyphosate. 3+ Complex fluorescent probes and their preparation methods. Due to the interaction between glyphosate and Al... 3+ The complexing ability is stronger than that of kaempferol and Al. 3+ Complexing ability of kaempferol-Al 3+ Adding glyphosate to the complex fluorescent probe allows glyphosate to abstract Al. 3+ The probe dissociates and exhibits obvious fluorescence quenching, enabling the detection of glyphosate based on this principle. The fluorescent sensing system constructed in this invention detects glyphosate without interference from other pesticides, and features simple preparation, high selectivity, high sensitivity, and fast response, achieving trace detection of glyphosate. Furthermore, test strips made from the fluorescent probe of this invention can visually detect glyphosate under ultraviolet light.

The inventors' primary objective was to provide a technology that suppresses inflammation, particularly in aging tissues. [Solution] The above problem can be solved by a composition containing at least one selected from the group consisting of genistein, quercetin, fisetin, nobiletin, kaempferol, apigenin, and naringenin, and glycosides thereof.

To provide a plant extract containing kaempferol aglycone, and a method for producing the same.SOLUTION: The plant extract contains kaempferol aglycone in an amount of ≥ 1mg / g expressed in terms of dried weight. The method for producing the extract comprises (1) extracting a plant raw material containing the kaempferol glycoside with a solvent and (2) hydrolyzing the extract obtained in the above (1).SELECTED DRAWING: None

The invention relates to the technical field of liqueur, in particular to liqueur and a preparation method thereof.The liqueur is prepared by soaking various medicinal and edible raw materials such as kudzuvine roots, rice, barbary wolfberry fruits, smoked plums, grapes, mulberries, poria cocos, Chinese yams, rhizoma polygonati, jujubes and ginseng in baijiu base liquor according to a specific proportion. According to the present invention, the modern analysis technology shows that the wine contains 25 active components such as resveratrol, protocatechuic acid, oleanolic acid, quercetin, kaempferol and the like, and the components achieve the synergistic effect, such that the product has effects of tumor resistance, liver protection, blood sugar reduction, blood fat reduction, oxidation resistance, inflammation resistance, nerve protection and other health-care functions, and the product has characteristics of definite component, synergistic effect, high safety, and no toxic-side effect. The preparation process is stable and controllable, and is suitable for large-scale production.

The purpose of this invention is to provide an alcoholic beverage that can suppress the bitterness produced by kaempferol and alcohol. An alcoholic beverage is characterized by containing 100 ppb to 15000 ppb of kaempferol, more than 50 ppb of γ-nonanolactone, and isoamyl alcohol.

Hyperthyroid diarrhea refers to hyperthyroid diarrhea, which is mostly intractable diarrhea, due to excessive thyroid hormone causing increased intestinal peristalsis excitability and diarrhea when the thyroid function is increased, which is caused by endocrine metabolic disorder leading to gastrointestinal function disorder. The present application studies and observes the treatment effect of kaempferol coupling derivative JC-1 on diarrhea of hyperthyroid model mice, and provides a new drug treatment option for diarrhea type colon dysfunction.

The application discloses a detection method of Yichangleng composition, and the method comprises the following steps: S1, preparation of reference solution; S2, preparation of test solution; S3, preparation of mixed control solution; S4, respectively, precisely sucking Yichangleng test solution of S2 and mixed control solution of S3; injecting into a liquid chromatograph; S5, exporting Yichangleng characteristic spectrum obtained in S4, selecting chromatographic peaks existing in chromatograms of Yichangleng solution as characteristic peaks; calculating relative peak area and relative retention time of each characteristic peak; and according to the retention time of the chromatogram of the mixed control solution, labeling the components of the peaks in the control characteristic spectrum, the method can be used for detecting gallic acid, vanillic acid, paeoniflorin, uncarine, thalictrifolin, 5-O-methylvisamminol glycoside, quercetin, kaempferol and atractylochide II, and can realize overall quality control of Yichangleng solution.

The invention relates to the technical field of biological medicine and skin aging resistance, in particular to a mesenchymal stem cell vesicle containing kaempferol and application of the mesenchymal stem cell vesicle. The mesenchymal stem cell vesicles of kaempferol are prepared through a mechanical extrusion method, the characteristic structure and biological activity of exosomes are kept, and Kaempferol is loaded in an auxiliary mode through continuous extrusion, so that an MSC-EVM (at) Kae composite system is formed. The compound has efficient drug entrapment efficiency, and can realize slow release and efficient delivery in vivo. After microneedle transdermal drug delivery, MSC-EVM (at) Kae can significantly improve UVB-induced skin aging, reduce ROS level, inhibit secretion of inflammatory factors, recover MMP / TIMP balance and promote collagen synthesis, thereby improving skin structure and barrier function. The invention also provides a bionic drug delivery system with high biocompatibility and high efficiency in treatment of skin photoaging, and a new technical approach is provided for application of natural micromolecules combined with mesenchymal stem cell exosome biomimetic bodies.

The invention belongs to the technical field of medicines, and particularly relates to application of hibiscus (the hibiscus is a plant hibiscus flower and an extract and an active monomer thereof) in preparation of medicines for treating stomach diseases (atrophic gastritis, gastric mucosal lesion and gastric cancer). The invention discloses application of hibiscus syriacus in treatment of gastric diseases. The application comprises at least one of chronic atrophic gastritis treatment, gastric mucosa protection and gastric cancer prevention. In-vitro experiments show that the shrub althea flower extract, shrub althea flower polyphenol, syringic acid (I), protocatechuic acid (II), kaempferol (III) and kaempferol-7-O-beta-D-glucoside (IV) have obvious antioxidant activity and gastric mucosa protection activity, can obviously reduce the ROS content in ethanol-induced GES-1 cells and the expression of apoptosis marker protein Caspase-3 / Bax, and have a wide application prospect.

The invention discloses a rapid safflower quality detection method based on an ultraviolet-visible-near infrared spectrum and a related device, and relates to the technical field of rapid traditional Chinese medicine quality detection.The method comprises the steps that firstly, spectral response data of a to-be-detected safflower sample at all wavelengths of an optimal characteristic wavelength set is obtained; the wavelength set is divided into subsets according to four quality indexes of hydroxysafflor yellow A content, kaempferol content, haematochrome absorbance and moisture content, and the wavelength of each subset is a wave band with the highest correlation of the corresponding indexes; secondly, respectively inputting the spectral data of each subset into a corresponding Levenberg-Marquardt algorithm prediction model, so as to obtain predicted values of four indexes; the problems that in the prior art, single-index detection is not comprehensive, and a traditional method is complex in pretreatment, long in detection period and high in equipment cost are solved, model fitting is accurate, complex operation is not needed, and the requirement for the professional level of personnel is lowered.

The application discloses a method for improving aglycone conversion efficiency by using a eutectic solvent and belongs to the technical field of green extraction. The method for improving aglycone conversion efficiency by using a eutectic solvent is characterized by comprising the following steps: uniformly mixing kaempferol-3-O-rutinoside, a eutectic solvent solution and a rutin degrading enzyme solution, performing an enzymolysis reaction and obtaining an enzymolysis solution; then, purifying the enzymolysis solution to obtain aglycone kaempferol; wherein the eutectic solvent in the eutectic solvent solution is one of betaine-ethylene glycol, choline chloride-urea, choline chloride-malic acid, choline chloride-urea-acetamide. The method is green and efficient, the prepared aglycone kaempferol has a high yield of more than 45% and a high purity of more than 95%.

The invention discloses a flavonoid composition for targeted regulation and control of TSLP-TSLPR and application of the flavonoid composition, the flavonoid composition is prepared from kaempferol, kaempferol-7-O-glucoside and kaempferol-7-O-rhamnoside, and the molar ratio of the kaempferol to the kaempferol-7-O-rhamnoside is (1-3): (1-3): (1-3). According to the composition, generation of key cytokines IL-4 and IL-13 of allergic inflammation is remarkably inhibited by blocking a TSLP-TSLPR signal channel, so that allergic skin diseases such as TSLP-mediated atopic dermatitis, urticaria and psoriasis are effectively treated. In action mechanism research, in-vitro experiments show that when the three components are combined according to the ratio of 2: 3: 1, the strongest inhibition effect on TSLP-induced IL-4 and IL-13 secretion in a HuT78 cell model is achieved, and the effect is remarkably superior to that of a single-component treatment group. According to the invention, the action limitation of a single flavone component is broken through, the efficient targeted regulation and control of the TSLP-TSLPR pathway is realized through the synergistic effect of multiple components with accurate proportioning, and a safe and effective treatment scheme is provided for allergic skin diseases.

The invention relates to the technical field of medicines, in particular to an iridium-kaempferol complex as well as a preparation method and application thereof. One Ir (Cp *) Cl2 structure in [Ir (Cp *) Cl2] 2 is substituted by one C15H10O6 to prepare the iridium-kaempferol complex, an Ir (III) center is combined with a kaempferol ligand for the first time, the metal organic complex Ir-Kae which not only utilizes the stability of the Ir (III) complex but also utilizes the pharmacological activity of kaempferol is developed, and through the synergistic effect of multiple mechanisms such as oxidation resistance, inflammation resistance and neuroprotection, the metal organic complex Ir-Kae can be used for preparing the metal organic complex. The spinal cord injury can be effectively treated at multiple target points. The Ir-Kae has a remarkable free radical scavenging capability, can effectively reduce the level of active oxygen in cells and organisms, realizes remarkable oxidation resistance and oxidative stress resistance, can improve the living and death degree of damaged hippocampal neurons, promotes neuronal repair, and has a remarkable anti-aging effect on hippocampal neurons. The nerve injury and the spinal cord injury are effectively treated, and a new direction is provided for assistant treatment of the spinal cord injury medicine.

Disclosed is a kaempferol mesenchymal stem cell membrane biomimetic material capable of more accurately targeting a damaged tissue. The biomimetic nanomaterial is simple to prepare, high in universality, and suitable for large-scale production. The present invention uses mesenchymal stem cell membranes to improve the biocompatibility and targeting property of the carrier and prolong the circulation time of the drug in vivo. The biomimetic nanomaterial prepared by the present invention enables targeted delivery of kaempferol to the liver, providing a novel treatment method for acute liver failure ALF.

The application discloses a connecting peptide and application thereof in promoting glucose methylation modification of a compound. Glycosyltransferase-methyltransferase fusion protein genes connected by connecting peptides, genes LtLasS1 and LtLasS2 encoding benzenediol lactone synthetase are co-expressed, and it is determined through liquid phase and mass spectrum analysis of fermentation products that the fusion protein can realize glucose methylation modification on benzenediol lactone. The conversion efficiency of the fusion protein using DL-4 connecting peptide on benzenediol lactone is the highest, and the relative conversion rate is 65.54+ / -3.75%. In addition, the glucose methylation conversion efficiency of the artificial fusion protein constructed by feeding the substrate kaempferol is tested, and the conversion efficiency of the fusion protein using DL-4 connecting peptide on the substrate is 6 times that of the conversion efficiency of the substrate by using a single glycosyltransferase and methyltransferase respectively, and the relative conversion rate is increased to 60.12+ / -4.79%.