489 results about "Kaempferol" patented technology

The present invention relates to compositions useful in treating hyperpigmentation and the various signs of dermatological aging in human skin. The present invention also relates to cosmetic compositions and methods of using such compositions that improve the aesthetic appearance of skin. Further, the present invention relates to methods of applying the compositions to the skin to effect treatment and to improve the aesthetic appearance of skin, particularly, by providing anti-aging benefits to the skin. These and other objects of the present invention are achieved by a method and composition that comprises (a) a de-pigmenting agent or anti-aging agent in an amount effective to prevent, treat and / or ameliorate pigmentation or the various signs of aging at an area of skin to which it is applied, and (b) a cosmetically or pharmaceutically acceptable vehicle. Suitable de-pigmenting agents include 3,3'-thiodipropionic acid, thiazolidine-2-carboxylic acid, Kaempferol-7-glucoside, perilla oil, and clofibrate and clofibrate analogs and / or derivatives, as well as those set forth below. Suitable anti-aging agents include 3,3'-thiodipropionic acid and / or its derivatives.

The present invention relates to the field of medicine technology. Safflower, crigeron bieriscapus, ginkgo leaf and other plant flower and leaf containing kaempferol and its derivative, rather than kaempferol and its derivative, have been used in preventing and treating cardiac and cerebral vascular diseases. The present invention verifies that kaempferol and its derivative has the functions of preventing and treating cardiac and cerebral vascular diseass via systemic pharmacological and pharmacodynamical experiments. Therefore, kaempferol and its derivative may be used in preparing medicine for preventing and treating cardiac and cerebral vascular diseases.

The invention relates to a composite of active ingredients of Chinese herb medicine used for preventing the damage of cerebral ischemia and relevant diseases, in particular to a preparation which is prepared by the active ingredients of the Chinese herb medicine; the preparation is prepared by the active ingredients of the Chinese herb medicine and the conventional preparation technique which is used by carriers permitted by pharmacy, and comprises the following components with the parts by weight: 2 to 10 parts of ginsenoside Rb, 2 to 10 parts of ginsenoside Rg1, 1 to 5 parts of ginsenoside Rd, 1 to 5 parts of ginsenoside Re, 2 to 10 parts of stilbene glycoside, 1 to 5 parts of ginkgolide and 1 to 5 parts of flavonoid mixture which consists of kaempferol and quercetin with the weight rate to be 1:1. The invention has the advantages that the defects that in the traditional compounded Chinese medicine, the composition is complex, the product quality is not controlled effectively and the curative effect is not stable are overcome, has clear ingredients, definite effect, stable quality and obvious control effect on ischemic stroke, sequela, cerebral arteriosclerosis and vascular dementia, can improve the functions of motor nerve, and learning and memory of the patients, has low toxicity and simple preparation method without obvious side effect and is suitable for industrial mass production.

The invention discloses a method of extracting flavonoid glycosides from grosvenor monordica leaves and a product thereof. The method comprises the following steps: taking Grosvenor monordica leaves, boiling the Grosvenor monordica leaves with water, carrying out extraction and filtration twice to three times, each extraction lasting 50 to 100 min, combining filtrates, carrying out adsorption on an obtained filtrate by using a macroporous resin, rinsing the resin with water until effluent is colorless, carrying out elution by using ethanol or absolute methanol, collecting eluent, removing ethanol or methanol, placing the eluent in a refrigerator for 20 to 30 h, allowing precipitate to deposit, dissolving the precipitate with absolute ethanol, carrying out filtration and evaporating an obtained filtrate so as to obtain a crude extract, kaempferol-3,7-a-L-dirhamnoside. The extraction method provided in the invention is simple, scientific and reasonable, has strong feasibility and enables an obtained extract product to have a high purity, wherein, the purity of a refined extract product is higher than 95%; the extract product obtained in the invention has good free radical scavenging activity and a wide application prospect. In addition, application of the method enables full utilization of considerable waste grosvenor momordica leaves to be realized, thereby improving the utilization rate of natural resources and reducing waste.

The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation and high purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.

The invention relates to a method for preparing kaempferol. The method comprises the following technical steps of: crushing raw materials, and extracting alkaline solution; performing enzymolysis after acid adjustment, and extracting enzymolysis liquid by using ethyl acetate; and performing concentration under reduced pressure on the extract liquor, and recrystallizing by using methanol to obtain the product. The method for producing the kaempferol has the advantages of simple process, high utilization ratio of raw materials, and high purity.

The invention discloses two types of flavonoid glycoside compounds which are kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(2'' ', 4'' '-O-diacetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-glucopyrose galactopyranoside and kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(4'' '-O-acetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-D-glucopyrose galactopyranoside respectively. The invention also discloses a method for preparing the two types of flavonoid glycoside compounds. Moreover, pharmacological tests prove that the two types of flavonoid glycoside compounds have quite good effects of reducing blood sugar and blood fat and resisting oxidation and cerebral ischemia, and thus, the flavonoid glycoside compounds of the invention can be used for preparing medicaments, food and cosmetics for preventing and treating diabetes, reducing blood fat and resisting oxidation and cerebral ischemia.

The invention relates to the technical field of natural medicine and particularly relates to the use of a flavonoid compound originated from Dracocephalum moldavica in protecting myocardial ischemia reperfusion injury. A pharmacology experiment proves that the effect of protecting myocardial ischemia reperfusion injury of lonicerin, kaempferol and galuteolin is better than that of other flavonoid compounds and than that of general flavone of Dracocephalum moldavica.

Lindley eupatorium herb total flavone and its pharmaceutically acceptable salt is used in preparing medicine for preventing and treating virus diseases, especially SARS. Lindley eupatorium herb total flavone is selected from quercetin, kaempferol, jaceidin, hyperoside, trifolirhizin, astragaloside or their mixture. Lindley eupatorium herb total flavone in the concentration of 100 microgram / ml has no toxicity to VeroE6 cell and complete supperssion on SARS virus.

The invention relates to a lycium ruthenicum murr anthocyanin chewable tablet which is prepared from the following components in parts by weight of 50-500 parts of lycium ruthenicum murr anthocyanin, 30-300 parts of peach flower kaempferol, 10-100 parts of rhizome of conic gymnadenia and 50-500 parts of lycium barbarum polysaccharide according to a conventional method comprising the steps of sequentially granulating, drying at the temperature of 40-50 DEG C, finishing, and tabletting under the condition that the pressure is 80-100kN. The lycium ruthenicum murr anthocyanin chewable tablet has the advantages that raw materials are easily available, industrial implementation is realized easily, and a human body can maximally utilize and absorb plant extracts.

There is provided a topical composition for treating, preventing or ameliorating hyperpigmentation in human skin. The composition has a de-pigmenting agent in an amount effect to reduce or diminish pigmentation at an area of skin to which it is applied, and a cosmetically or pharmaceutically acceptable vehicle. Suitable de-pigmenting agents include 3,3'-thiodipropionic acid, thiazolidine-2-carboxylic acid, kaempferol-7-glucoside, perilla oil, and clofibrate and clofibrate analogs and derivatives. There is also provided methods for treating, preventing or ameliorating hyperpigmentation in human skin.

The invention belongs to the technical field of tea production, and particularly relates to a Tetrastigma hemsleyanum Duels et Gilg tea with high contents of Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide and kaempferol, and a manufacturing method of the Tetrastigma hemsleyanum Duels et Gilg tea. The method comprises the following steps: (1), spreading fresh Tetrastigma hemsleyanumDuels et Gilg leaves for 10-20 minutes; (2), fixation: deactivating enzymes of the Tetrastigma hemsleyanum Duels et Gilg leaves by adopting a microwave tea fixation drier; (3), flat pressing for shaping: uniformly spreading the Tetrastigma hemsleyanum Duels et Gilg leaves subjected to fixation on a bamboo skin board, and pressing another bamboo skin board on the leaves to enable the cooled Tetrastigma hemsleyanum Duels et Gilg leaves to be shaped; and (4), drying: drying the semi-finished products processed in the step (2) by a microwave tea machine. The Tetrastigma hemsleyanum Duels et Gilg tea is natural green, is bright green in color and light green in grounds, does not contain caffeine, is rich in Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide, kaempferol and other ingratiates, can effectively inhibit cancer cell proliferation, promotes cancer cell poptosis, has no toxic or side effects and is easy to absorb and beneficial to health. The Tetrastigma hemsleyanum Duels et Gilg leaves are tender, have less fibers, and can be eaten after being brewed.

The invention provides a method for preparing kaempferol-3-O-2'',6''-dirhamnosylglucoside, which comprises the following steps: by using a two-dimensional liquid phase chromatography-mass spectroscopy combination technology and taking methanol-water or acetonitrile-water as a mobile phase and a reversed phase C18 chromatographic column as a one-dimensional preparative chromatographic column, performing component cutting on a ginkgo leaf extract, and collecting a target component which is a kaempferol-3-O-2'',6''-dirhamnosylglucoside crude product; and by taking a reversed phase C18 chromatographic column as a two-dimensional preparative chromatographic column, under the guidance of selective ion peaks of mass spectroscopy, performing component cutting on the kaempferol-3-O-2'',6''-dirhamnosylglucoside crude product, collecting, and concentrating through rotary evaporation to obtain the high-purity kaempferol-3-O-2'',6''-dirhamnosylglucoside, wherein the purity can be up to 70% or above. According to the invention, the preparation process is high in repetitiveness and favorable in operability; and meanwhile, ginkgo leaves are abundant in resources and easy to acquire. Thus, the invention meets the requirements for large-scale production and can be used for the preparation of raw materials for a Shuxuening injection.

The invention provides a Chinese herbal medicine phagostimulant used for hucho salmon, which relates to a phagostimulant used for fishes. The invention solves the problem that no phagostimulant suitable for hucho salmon exists at present. The Chinese herbal medicine phagostimulant comprises asafoetida giantfennel, pihancao, aniseed, cumin, tangerine peel and kaempferol. The preparation method of the Chinese herbal medicine phagostimulant comprises the steps of pulverizing asafoetida giantfennel, pihancao, aniseed, cumin, tangerine peel and kaempferol and mixing evenly. The Chinese herbal medicine phagostimulant used for hucho salmon has good palatability to hucho salmon, can improve the taste of a feed with low fish meal, enhances the growth performance and has no pollution with simple processing process, and the addition quantity in a fish feed is 0.1-0.5%. The invention can be applied to the cultivation of hucho salmon, the rate of liveweight growth of hucho salmon is enhanced by 30-40%, the grazing rate is enhanced by 20-35%, the survival rate is enhanced by 0.5-2%, and the bait coefficient is lowered by 5-8%.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The invention discloses the applications of compounds, selected from quercetin, chrysophanic acid, digicitrine, formononetin, honokiol, berberine, kaempferol, bergenin, deoxyepinephrine and licochalcone A, as well as derivatives thereof, in preparation of medicines applied to methods that are used for promoting stem cell proliferation, delaying senescence of stem cells, promoting differentiation of the stem cells into osteoblasts, chondrocytes and adipocytes, enhancing the vascularization capability of the stem cells and / or improving exercise tolerance of mice. The invention also discloses that the compounds can be combined with vitamin C for application.

The invention relates to the technical field of medicaments, in particular to novel application of kaempferol as a synergist of anti-fungal medicaments. The anti-fungal medicaments are azole-type or polyene-type anti-fungal medicaments and based on the effective concentration of the anti-fungal medicaments, the adding ratio of the kaempferol is 0.5 to 16 mu g / ml. Tests show that when the kaempferol and the anti-fungal medicaments such as fluconazole, ketoconazole, miconazole and amphotericin B are used together, not only the anti-fungal effect is guaranteed on the premise of lowering the consumption of the anti-fungal medicaments, but also the anti-fungal medicaments can restore the function of killing the drug-resistant fungi, so that the kaempferol can be used as a synergist of the anti-fungal medicaments. The kaempferol used as the synergist of the anti-fungal medicaments can lower the consumption of the azole-type or polyene-type anti-fungal medicaments so as to reduce the toxic and side effects of the medicaments; and the kaempferol can make the anti-fungal medicaments restore the function of killing the drug-resistant fungi, so that the kaempferol can effectively treat mycotic infection, particularly drug-resistant t mycotic infection, and has quite important clinical application values.

The invention relates to the technical field of plant genetic engineering, and provides a gene segment DNA capable of assigning capacities to solanaceae accumulated flavonoids such as rutin, kaempferol rutin and naringenin chalcone, and caffeoyl quinic acid, wherein the gene segment has a gene sequence shown in SEQ ID NO:1, and is obtained from tomato, and comprises a control gene S1MYB12 for controlling the synthesis of solanaceae flavonoids and caffeoyl quinic acid. The inventor provides a specific method of separation cloning, function verifying and applying of the gene segment. A tomato endogenous gene S1MYB12 is driven to be specifically expressed in the tomato fruit by using a tomato fruit specificity expression promoter E8, thus the contents of the flavonoids and the caffeoylquinic acid in the tomato fruit are greatly increased, and the function of the tomato endogenous gene S1MYB12 of regulating the synthesis of the flavonoids and the caffeoyl quinic acid is verified.

The invention provides a method for preparing kaempferol-3-O-rutinoside from a ginkgo leaf extract, which comprises the following steps: by using a two-dimensional liquid phase chromatography-mass spectroscopy combination technology and taking methanol-water or acetonitrile-water as a mobile phase and a reversed phase C18 chromatographic column as a one-dimensional preparative chromatographic column, performing component cutting on a ginkgo leaf extract, and collecting a target component which is a kaempferol-3-O-rutinoside crude product; and by taking a hydrophilic TECys as a two-dimensional preparative chromatographic column, performing component cutting on the kaempferol-3-O-rutinoside crude product, collecting, and concentrating through rotary evaporation to obtain the high-purity kaempferol-3-O-rutinoside, wherein the purity can be up to 80% or above. According to the invention, the preparation process is high in repetitiveness and favorable in operability; and meanwhile, ginkgo leaves are abundant in resources and easy to acquire. Thus, the invention meets the requirements for large-scale production and can be used for the preparation of raw materials for a Shuxuening injection.

An application of kaempferol and its derivatives in preparing the medicines for preventing and treating depression and dementia is disclosed.

The invention provides a method for comprehensively extracting dye lignin and kaempferol from sophora fruit. The technical concept is shown in the steps as follows: 1, crushing raw materials, hydrolyzing through acid water, and extracting acidolysis slag through an organic solvent; 2, combining an extracting liquid, and concentrating and filtering the extracting liquid to obtain precipitate which is a mixture of kaempferol and dye lignin; 3, extracting the obtained precipitate through alkaline liquid, and centrifugally separating to obtain the precipitate which is kaempferol and obtain the supernate which is dye lignin; 4, dissolving the obtained precipitate through the organic solvent, and adding an absorbent to be purified, and filtering, concentrating, and centrifugally separating to obtain faint yellow powder which is kaempferol; and 5, discoloring the supernate obtained in step 3 through the absorbent, and filtering, then adjusting the pH (Potential Of Hydrogen), standing and precipitating, and centrifugally separating to obtain the white powder which is the dye lignin. With the adoption of the method provided by the invention, the problem of the prior art that the resource is wasted because two products cannot be comprehensively developed can be overcome, the extracting step is optimized, the producing cost is reduced, and the operability is improved.

The invention relates to detection of flavonoids in tobacco, in particular to a metabonomics method for detecting multiple flavonoids in fresh tobacco leaves.The method is characterized in that flavonoids in the tobacco leaves are extracted through an ultrasonic extraction method, and rutin, quercetin, naringenin, kaempferol glucoside, astragalin, naringin and isoliquiritigenin in the fresh tobacco leaves are detected through UPLC-QqQ-MS / MS.Compared with the prior art, the method has the advantages that accuracy and sensitivity are high, repeatability is good, and the method is suitable for simultaneously and quantitatively detecting the seven flavonoid compounds in plants and can be used for research on metabolic pathways and gene functions of plant flavonoid matter.

The invention discloses a drug for treating coronary heart disease, which comprises 230 of salvia, 70 of piper longum, 115 of aquilaria, 51 of rosewood, 76 of nutmeg, 38 of kaempferol, 76 of wide jujube, 25 of sandalwood, 76 of sea buckthorn, and 25 of red sandal according to the weight ratio. The salvia, the piper longum, the kaempferol, the wide jujube, the sea buckthorn, and the nutmeg are extracted for three times; for the first time, 14 times of 75 percent of ethanol are added and heated with 2 hours of reflux; for the second time, 12 times of 75 percent of ethanol are added and heated with 2 hours of reflux; for the third time, 10 times of 75 percent of ethanol are added and heated with 2 hours of reflux and the ethanol is recycled. After the coarse powder of aquilaria, rosewood, red sandal and sandalwood are immersed into 8 times of water for 5 hours, volatile oil is extracted for twice with 14 hours for each time; the water extract liquid of the volatile oil is filtered; the herb residue is added with 8 times of water and then heated and recycled for 2 hours, and then filtered; later the extract liquid is merged, concentrated, and dried for obtaining cream powder. By crushing the cream powder into fine powder and then mixing evenly the powder, later adding suitable amount of PEG-6000 for melting and then adding the volatile oil, dropping pills are then made.

There is provided a topical composition for treating, preventing or ameliorating hyperpigmentation in human skin. The composition has a de-pigmenting agent in an amount effect to reduce or diminish pigmentation at an area of skin to which it is applied, and a cosmetically or pharmaceutically acceptable vehicle. Suitable de-pigmenting agents include 3,3'-thiodipropionic acid, thiazolidine-2-carboxylic acid, kaempferol-7-glucoside, perilla oil, and clofibrate and clofibrate analogs and derivatives. There is also provided methods for treating, preventing or ameliorating hyperpigmentation in human skin.

The invention discloses application of kaempferol in preparation of medicines for preventing and treating radiation-induced pulmonary injury. The experiment proves that kaempferol can obviously improve inflammatory exudation of lung tissues suffering from the mouse radiation-induced pulmonary injury and has an excellent positive effect on the radiation-induced pulmonary injury. The invention provides a novel drug for radiation-induced pulmonary injury diseases. A novel thought is provided for research and development of medicines for treating the radiation-induced pulmonary injury, and the application path of kaempferol in the field of medicines is widened.

The invention discloses an analysis method for simultaneously determining six polyphenol content in flue-cured tobacco. The method comprises the following steps: grinding leaves of flue-cured tobacco, extracting through a methanol solution, analyzing filtrate through a high performance liquid chromatography after filtering, adopting a HSST3 chromatographic column and a diode array detector, wherein the detection wavelength of the detector is 342mm, the reference wavelength is 480nm, the bandwidth of the detection wavelength is 4nm, the bandwidth of the reference wavelength is 100nm, and the flow velocity of the flowing phase is 0.4mL / min; the flowing phase A is aqueous solution of glacial acetic acid, and the flowing phase B is methanol solution of glacial acetic acid, simultaneously determining contents of six polyphenols in the flue-cured tobacco: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopoletin, rutin and kaempferol alcohol-3-rutinoside in a gradient elution manner. The adding standard recovery of the method is between 95.6-109.3%, the detection limit is 3.7-9.9 migrogram / g, the relative standard deviation is 0.5-3.9% (n equals to 4), the relative coefficients of the standard solution are more than 0.9995, and the pre-treatment of the method is simple and the analysis time is short.

The invention relates to a preparation method and application of camellia flavonoid for reducing blood glucose. The camellia flavonoid is kaempferol-3-0-[2-O-beta-D- xylose-6-O-alpha-rhamnose]-beta-D-glucoside. Compared with the prior art, the preparation method of the camellia flavonoid for reducing the blood glucose has the following beneficial effects that seed cakes obtained after the camellia seed is squeezed for oil extraction are applied to the preparation and separation of the active ingredient for reducing the blood glucose; and the camellia flavonoid obtained by using the preparation method provided by the invention can be used as a medicine and healthcare food for diabetes mellitus, has good blood glucose reducing effect and hardly damages a human body.

The present disclosure generally relates to nutritional compositions that are suitable for administration to adult and pediatric subjects that include a neurologic component. The neurologic component may include a flavonoid compound, such as kaempferol, fisetin, or both, and provides beneficial health benefits such as enhanced brain development and improved memory, cognition, hand-eye coordination, and enhanced focusing. Furthermore, the neurologic component may act synergistically with other brain nutrients that may be present in the compositions.

The invention provides an extractive from juglans mandshurica maxim bark, which mainly has the following active components: 8-hydroxide radical-9, 10- anthraquinone-1- carboxylic acid, regiolone, 4, 5, 8-trihydroxy-alpha-naphthalenone-3-O-beta-D-glucopyranoside, 4, 5, 8-trihydroxy-alpha-naphthalenone-3-O-(6'-O-galloyl-group)-beta-D-glucopyranoside, 1-(4'-hydroxyphenyl)-7-(3'- methoxy-4'-hydroxyphenyl) heptane-3- ethanol, 4- hydroxide radical-2, 6- syringol-1-O-beta-D-glucopyranoside, 4- hydroxide radical-2, 6- syringol-1-O-(6'-O-galloyl group)-beta-D-glucopyranoside, vanillic acid-4-O-(6'-O-galloyl group)-beta-D- glucopyranoside, vanillic aldehyde, vanillic acid, gallic acid, syringic acid, kaempferol and quercetin. The extractive from the juglans mandshurica maxim bark has good effects of treating hepatopathy and inhibiting the tumor growth and particularly has direct killing effect on liver cancer cells.

The invention discloses a preparation method of a double drug-loading fluorescent magnetic microsphere composite system. The preparation method comprises the following steps: (1) synthetizing a magnetic kaempferol microsphere according to a sonochemical method; (2) synthetizing an amination graphene quantum dot by a hydrothermal method and achieving loading of paclitaxel; (3) connecting the graphene quantum dot loaded with paclitaxel to the magnetic kaempferol microsphere through aminocarboxylic reaction to obtain the fluorescent magnetic microsphere composite system loaded with a kaempferol drug and a paclitaxel drug. In the prepared fluorescent magnetic microsphere composite system, the graphene quantum dot loads paclitaxel through Pi-Pi physical action, and the magnetic kaempferol microsphere loads kaempferol through physically trapping to achieve double drug-loading. The prepared fluorescent magnetic microsphere composite system loads two anti-cancer drugs of paclitaxel and kaempferol, and kaempferol can remarkably enhance the sensibility of human cervical cancer Hela cells to a chemotherapy drug, namely paclitaxel, and the double drug-loading fluorescent magnetic microsphere composite system has the advantages that the treatment effect can be improved, the generation of the drug resistance is delayed, and toxic or side effects are reduced greatly.