234 results about "Kaempferol" patented technology

The invention relates to the technical field of biology, in particular to application of N-terminal truncated 8-isopentenyl transferase in production of 8-isopentene kaempferol. The invention provides the N-terminal truncated 8-isopentenyl transferase, and the N-terminal truncated 8-isopentenyl transferase can be used for carrying out isopentenylation on kaempferol to generate 8-isopentene kaempferol. Specifically, the saccharomyces cerevisiae is used as a chassis, under the condition that kaempferol is exogenously added, through overexpression of 8-isopentenyl transferase, biosynthesis of 8-isopentenyl kaempferol in the saccharomyces cerevisiae is realized, and meanwhile, a foundation is laid for subsequent biosynthesis of icaritin. The invention provides a novel reference scheme for biosynthesis of flavonoid compounds.

The invention discloses a preparation method of a novel ginseng exosome nano drug delivery system, and mainly relates to the field of functional medical materials. Comprising the following steps: S1, preparing a ginseng exosome crude extract; s2, centrifuging the crude extract and taking supernate; s3, centrifuging the supernate, and taking the precipitate to obtain an exosome resuspension; s4, preparing a purified ginseng exosome; s5, preparing kaempferol nano-enzyme freeze-dried powder; s6, mixing the ginseng exosome solution with the kaempferol nano-enzyme freeze-dried powder to obtain a ginseng exosome kaempferol nano-enzyme mixed solution; and S7, separating the ginseng exosome loaded with the kaempferol nano enzyme to obtain the ginseng exosome nano delivery system. The preparation method has the beneficial effects that the plant active ingredient ginseng exosome is taken as a delivery carrier to carry the water-soluble kaempferol nano-enzyme, and the water-soluble kaempferol nano-enzyme and the ginseng exosome play a synergistic role, so that the effective loading capacity of the exosome on the medicine is improved, the concentration of the medicine is improved, and the preparation method plays a role in various diseases such as wounds and inflammation; the traditional Chinese medicine composition can be better applied to treatment of acute lung injury.

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention provides application of a natural compound in prevention and treatment of gray mold of fruits and vegetables. In the invention, botrytis cinerea can be inhibited by using the natural compound kaempferol. Experimental results show that the inhibitor has an inhibition effect on botrytis cinerea, has an IC50 value of 64 [mu] g / mL, has a good prevention and treatment effect on gray mold of cherry tomatoes, delays rotting and deterioration of the cherry tomatoes and prolongs the shelf life. The effect of the natural compound kaempferol in inhibiting the toxicity of botrytis cinerea is expected to become a novel prevention and treatment means for gray mold of fruits and vegetables.

The invention discloses a carrier-free nano-particle with pH / ROS dual response as well as a preparation method and application of the carrier-free nano-particle. The preparation method comprises the following steps: enabling quinolizidine alkaloid and flavonol to be subjected to self-assembly, so as to prepare quinolizidine alkaloid-flavonol carrier-free nano particles; modifying the quinolizidine alkaloid-flavonol carrier-free nano particles by adopting a phenylboronic acid-hyaluronic acid conjugate, so as to prepare nano particles modified by the phenylboronic acid-hyaluronic acid conjugate; and coating the surfaces of the nano-particles modified by the phenylboronic acid-hyaluronic acid conjugate with a pH sensitive polymer to prepare the pH / ROS dual-response carrier-free nano-particles. The sophocarpidine-kaempferol carrier-free nanoparticles with pH / ROS dual response, prepared by the invention, have the advantages of good targeting property, high biocompatibility, small toxicity and adverse reaction and the like, meanwhile, the nanoparticles have a good anti-inflammatory effect, and a new choice is provided for the treatment of ulcerative colitis.

The invention belongs to the technical field of biological medicine, and particularly discloses application of kaempferol in preparation of a medicine for treating chronic obstructive pulmonary disease. The invention provides application of kaempferol in preparation of a medicine for treating chronic obstructive pulmonary disease. The invention provides a medicine for treating chronic obstructive pulmonary disease. The medicine comprises kaempferol or pharmaceutically acceptable salt thereof as an active ingredient. The invention aims to provide the application of kaempferol in preparation of the medicine for treating the chronic obstructive pulmonary disease, and the chronic obstructive pulmonary disease is treated by adjusting an AMPK signal channel, a Hippo signal channel, an MAPK signal channel and a PI3K-AKT signal channel.

The invention discloses application of kaempferol in promotion of gene editing, and belongs to the field of biological medicine. The application specifically comprises kaempferol as an accelerant for CRISPR (clustered regularly interspaced short palindromic repeats) gene editing and application of kaempferol in preparation of non-viral site-specific integrated CAR T cells. The invention finds that the kaempferol can significantly increase the CRISPR gene editing efficiency, and cell experiments find that the kaempferol has a significant promotion effect on the positive rate of CAR T cells after electrotransfection, and the tumor killing function of the T cells is greatly improved.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

An EAE mouse model proves that the kaempferol and the ephedra, radix aconiti carmichaeli and herba asari particles can obviously save the myelin sheath damage of a mouse. Meanwhile, through serum metabolome analysis and metabolome and transcriptome analysis, the binding force of kaempferol and MMP3 is predicted, and through an EAE mouse model, it is proved that kaempferol can reduce expression of MMP3 through binding with MMP3, and IL-17 and demyelination are inhibited, so that the disease progression of EAE is delayed. Therefore, the invention proves that the kaempferol and the ephedra, monkshood and asarum granules can be used for treating and / or relieving the multiple sclerosis (MS), and the invention provides a new way for the treatment of the multiple sclerosis (MS).

The invention discloses a mesenchymal stem cell membrane coated kaempferol bionic nano-drug as well as a preparation method and application thereof, and relates to the technical field of targeted drug preparation, the bionic nano-drug is composed of nano-particles containing kaempferol and a mesenchymal stem cell membrane coated outside the nano-particles, the mesenchymal stem cell membrane is derived from umbilical cord mesenchymal stem cells; the particle size of the bionic nano-drug is 167.67 + / -1.84 nm, the PDI is 0.181 + / -0.027, the potential is-5.41 + / -0.74 mV, the encapsulation efficiency is 79.97 + / -0.19%, and the entrapment rate is 7.27 + / -0.02%. According to the invention, the water solubility of the kaempferol is enhanced, the specific enrichment of the kaempferol at the diseased region of the AS can be enhanced, the utilization efficiency of Kae is improved, and the defects of small molecular drugs are overcome; in addition, through nanocrystallization of Kae, direct contact of cells and tissues with Kae can be reduced, and cytotoxicity is reduced.

The invention belongs to the technical field of medicines, and particularly relates to a flavonoid compound-meglumine co-amorphous substance and a preparation method thereof. The flavonoid compounds comprise baicalein, hesperetin, naringenin, kaempferol, quercetin, isoliquiritigenin and the like, and the co-amorphous substance consists of a single flavonoid compound and meglumine and is prepared by adopting a melting shock cooling method or a rotary evaporation method. The miscibility of the flavonoid compounds and the meglumine is screened through Hansen solubility. The crystal diffraction characteristics, thermodynamic properties and intermolecular interaction of the co-amorphous substance are measured through PXRD, DSC and FTIR, and it is shown that the six flavonoid compounds and meglumine successfully form a co-amorphous system. The solubility and the dissolution rate of the six flavonoid compound-meglumine co-amorphous substance are obviously superior to those of flavonoid bulk drugs, and the physical stability of the formed co-amorphous substance is obviously superior to that of a single amorphous drug, so that the co-amorphous substance shows good patent medicine performance.

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention discloses kaempferol liposome nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that firstly, liposome is prepared, kaempferol is slowly pumped into the liposome, liquid is subjected to ultrasonic treatment and stirring during pumping, water bath rotary evaporation is conducted till an organic solvent is volatilized, then deionized water is added, and the kaempferol liposome nanoparticles are obtained. And stirring until the mixture is completely hydrated to form a faint yellow emulsion, filtering, and freeze-drying to obtain powder. The prepared and synthesized kaempferol lipidosome can improve the bioavailability of kaempferol, so that the kaempferol lipidosome has a regulating effect on inflammation of traumatic ectopic ossification lesion parts and inhibits ectopic bone formation. In-vitro experiments prove that the kaempferol liposome nanoparticles can effectively inhibit LPS / IL-4 induced macrophage polarization, inhibit release of related cell factors and inhibit formation of ectopic bones. In-vivo studies show that the nanoparticles have an obvious improvement effect on traumatic ectopic ossification of rats, are good in biocompatibility and can be used for clinical treatment of traumatic ectopic ossification.

The invention discloses an on-line extraction self-optimization method for acanthopanax sessiliflorus flavone based on reinforcement learning. According to the method, multi-source data in the extraction process is collected in real time, and a fusion feature vector is generated after preprocessing; establishing a mapping relation from the feature vector to the contents of rutin, quercetin, kaempferol and hyperoside by using an offline test sample, and constructing a virtual measurer capable of outputting a target flavone composition vector and uncertainty thereof; constructing a safety domain boundary according to a solvent flash point temperature, an equipment tolerance temperature upper limit and a component thermal degradation temperature threshold, and calculating a time-varying safety constraint threshold in combination with uncertainty; performing linkage optimization on the temperature, the ethanol volume fraction, the solid-to-liquid ratio, the soaking time and the circulating flow by adopting a reinforcement learning strategy, and comprehensively considering flavone composition deviation and solvent energy consumption by a reward function; and finally, outputting the optimized parameters to extraction equipment for execution. According to the method, online, lossless and self-adaptive process optimization control is realized, inter-batch fluctuation is remarkably reduced, and the resource utilization efficiency is improved.

A method for constructing a chicory-poria compound fingerprint, comprising: (1) preparing a test solution from a chicory-poria compound; (2) using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swertiamarin, gentiopicroside, sweroside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissin to prepare a mixed reference solution; and (3) respectively injecting the test solution and the mixed reference solution into a high performance liquid chromatograph, and performing similarity analysis on the obtained chromatograms to obtain a chicory-poria compound fingerprint. Also provided is a use of the chicory-poria compound fingerprint obtained by means of the construction method in identifying and / or evaluating the quality of a chicory-poria compound. The quality of the chicory-poria compound can be comprehensively, objectively and accurately tested and evaluated, thereby ensuring the safety and effectiveness of patient medication.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention belongs to the field of preparation analysis, and discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of detoxification and hematogenesis granules. According to the invention, a method of combining the HPLC fingerprint spectrum and the chemical mode of the detoxification and hematogenesis granules is established, the contents of the deacetylasperuloside, the rosmarinic acid, the scutellarin, the luteolin, the apigenin and the kaempferol in the detoxification and hematogenesis granules are detected at the same time, and a comprehensive quality evaluation model of the detoxification and hematogenesis granules with different batch numbers is established, so that the analysis result is comprehensive and objective; the method is simple, convenient, reliable, good in repeatability, accurate, stable and high in sensitivity, the multi-component content determination method provides scientific support for quality supervision of the Zhuang medicine preparation detoxification and hematopoiesis granules, and the method can be used for comprehensive quality evaluation and control of the detoxification and hematopoiesis granules. Therefore, the production stability of product quality and the safety and effectiveness of clinical medication are ensured.

Described herein, inter alia, are compositions comprising and methods of making and using endothelial cells (ECs) comprising one or more mitophagy-inducing agents (e.g., rapamycin, SKF-96365, lithium, carbamazepine, latrepirdine, spermidine, resveratrol, sodium valproate, trehalose, kaempferol, metformin, AICAR, nilotinib, ambroxol, curcumin, and VL-004). Also described herein, inter alia, are methods vascular cell therapy, promoting blood vessel formation, and increasing vascular development, angiogenesis, and cell junction via administration of a population of ECs comprising a mitophagy-inducing agent (MIA-ECs).

The invention provides a quality detection method of antiviral particles. The quality detection method adopts HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) to simultaneously determine the following components: forsythiaside A, patchouli ketone, verbascoside, mangiferin, 5-methoxy-8-hydroxypsoralen, curcumenol, luteolin-7-glucuronide, kaempferol-3-O-rutinoside, baicalin, rutin and bergamot phenol. The detection method is a quantitative determination method for 11 active components in the antiviral particles, the detection method is short in time and low in cost, the quantitative analysis method is expected to provide reference for quality control of the antiviral particles, and the clinical curative effect and safety of the antiviral particles are guaranteed.

The invention relates to the technical field of health food, in particular to a composition and application thereof in maintaining the healthy level of blood sugar. The composition comprises rutin, quercetin, kaempferol and pectin. Experiments show that the composition obviously reduces the activity of alpha-glucosidase and obviously delays the digestibility of starch, so that the postprandial blood sugar level of mice is reduced, and the effect of the composition is obviously superior to that of other flavonoid and pectin compositions. It is shown that after the rutin, the quercetin, the kaempferol and the pectin are compounded according to a certain proportion, an obvious synergistic effect is generated. Besides, according to the composition prepared by the invention, the dosage of quercetin is reduced, the utilization rate of pectin is increased, the production cost is reduced, the raw materials are pure natural, safe and non-toxic, the functionality is strong, the cost is low, and the composition has a wide application prospect in the aspect of deep processing of starch-based healthy foods.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The application discloses an application of kaempferol in promoting gene editing and belongs to the field of biological medicines. The application specifically comprises kaempferol as a promoter of CRISPR gene editing and the application of kaempferol in preparing non-virus site-specific integration CAR T cells. It is found that kaempferol can significantly increase the efficiency of CRISPR gene editing, and it is found through cell experiments that kaempferol has a significant promoting effect on the positive rate of CAR T cells after electrotransformation, and greatly improves the tumor killing function of T cells.

The invention belongs to the technical field of traditional Chinese medicine preparation processing, and particularly relates to a safflower extract, safflower formula granules and a method for preparing the safflower extract and the safflower formula granules based on a pure electric drive evaporation low-temperature concentration technology. According to the preparation method of the safflower extract and the safflower formula granules, the safflower is extracted by adding water, and a pure electroconcentration technology is adopted, so that the content and the transfer rate of hydroxysafflor yellow A and kaempferol + 6-methoxy kaempferol in the safflower formula granules can be remarkably increased, the concentration temperature is low, the time is short, and the energy consumption is low.

The present invention relates to the field of medical technology, and more particularly to a method for constructing a characteristic spectrum for detoxification capsules. The present invention improves existing detoxification capsule quality standards by enabling simultaneous detection of chebulic acid, syringin, kaempferol-30-rutinoside, β-asarone, and asaraldehyde. Furthermore, the components of the formulation, such as chebulic acid, syringin, kaempferol-30-rutinoside, β-asarone, and asaraldehyde, are controlled, further ensuring the safety, uniformity, stability, and controllability of the product quality.

The application relates to kaempferol cerium nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines. The kaempferol cerium nanoparticles are prepared by performing coordination co-assembly on kaempferol and cerium ions in the presence of polyethylene glycol at a specific molar ratio, and the nanoparticles have uniform particle sizes and good dispersity. The nanoparticles have the anti-inflammatory activity of kaempferol and the ROS scavenging capacity of cerium ions, and show a significant synergistic effect in in-vivo and in-vitro experiments, can effectively reduce lung inflammation, inhibit oxidative stress, improve lung function, and have good biological safety, thereby providing a new drug candidate scheme for the treatment of ARDS.

The invention discloses a connecting peptide and application thereof in promoting compound glucose methylation modification. A glycosyl transferase-methyltransferase fusion protein gene and genes LtLasS1 and LtLasS2 coded by benzenediol lactone synthetase, which are connected in series by connecting peptides, are co-expressed, and liquid phase and mass spectrometry of a fermentation product are carried out to determine that the fusion protein can realize glucose methylation modification by hydroquinone lactone. Wherein the conversion efficiency of the fusion protein hydroquinone lactone using the DL-4 connecting peptide is the highest, and the relative conversion rate is 65.54 + / -3.75%. Besides, by feeding a substrate kaempferol and testing the glucose methylation conversion efficiency of the artificial fusion protein constructed by the connecting peptide, the conversion efficiency of the fusion protein using the DL-4 connecting peptide to the substrate is 6 times of the conversion efficiency of the fusion protein using a single glycosyl transferase and a single methyltransferase to the substrate, and the relative conversion rate is increased to 60.12 + / -4.79%.

The invention discloses a flavone composition capable of preventing and improving atopic dermatitis and application of the flavone composition, and belongs to the field of medicines and cosmetics. Cell morphology observation and beta-hexosaminidase detection prove that apigenin and chrysin have a good effect of inhibiting large cell strain degranulation; scratch experiments prove that kaempferol has a good effect of promoting interstitial closure of human keratinocytes, and then detection of atopic dermatitis related factor expression discovers that apigenin, chrysin and kaempferol are compounded to have the potential of enhancing the soothing effect. And human body safety evaluation and efficacy experiments are further carried out to screen out the optimal proportion of the soothing effect. The invention explores the multi-target synergistic prevention and improvement effect of the flavonoid compound on the aspects of allergy resistance, inflammation resistance and barrier repair, and expands the application and development value of the flavonoid compound in soothing cosmetics.

The invention discloses a longan leaf granule which is prepared by the following steps: adding water into longan leaves, heating, extracting, filtering, concentrating, drying, crushing to obtain dry paste powder, adding a diluent into the dry paste powder, uniformly mixing, adding a wetting agent, granulating, and drying to obtain the longan leaf granule. The forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the granules all meet the requirements, and the granules are good in stability, suitable for industrial batch production, capable of treating diabetes, capable of meeting the requirements of doctors for treatment based on syndrome differentiation and increase and decrease along with symptoms and convenient to carry and take by patients. Thin-layer chromatography is adopted for qualitative identification of the longan leaf granules, high performance liquid chromatography is adopted for content determination of quercetin, quercitrin and kaempferol in the longan leaf granules, operation is easy, convenient and accurate, repeatability is good, and a scientific basis is provided for quality evaluation and control of the longan leaf granules.

The invention discloses a copper ion-kaempferol nano-composite and application thereof in treatment of inflammatory bowel disease, the obtained nano-composite has very strong stability and active oxygen scavenging ability, and has excellent oxidation resistance and anti-inflammatory ability, and the specific performance is as follows: the surface is negatively charged, and the nano-composite can specifically target the diseased region of intestinal inflammation, so that the copper ion-kaempferol nano-composite can be applied to treatment of inflammatory bowel disease. The intestinal mucosal barrier injury is effectively repaired, the imbalance state of intestinal flora is adjusted, and the intestinal inflammation microenvironment is remarkably improved; the antioxidant and anti-inflammatory capacities of cells can be enhanced by regulating and controlling a PI3K-Akt signal channel and a nuclear factor kappa B (NF-kappa B) signal channel; the compound shows an excellent treatment effect in mouse models of typical inflammatory bowel diseases such as ulcerative colitis and Crohn's disease and related complications of bowel diseases. The invention provides a new way for constructing a multi-target and multi-layer treatment platform for the inflammatory bowel disease of a natural product.