186 results about "Kaempferol" patented technology

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention discloses a carrier-free nano-particle with pH / ROS dual response as well as a preparation method and application of the carrier-free nano-particle. The preparation method comprises the following steps: enabling quinolizidine alkaloid and flavonol to be subjected to self-assembly, so as to prepare quinolizidine alkaloid-flavonol carrier-free nano particles; modifying the quinolizidine alkaloid-flavonol carrier-free nano particles by adopting a phenylboronic acid-hyaluronic acid conjugate, so as to prepare nano particles modified by the phenylboronic acid-hyaluronic acid conjugate; and coating the surfaces of the nano-particles modified by the phenylboronic acid-hyaluronic acid conjugate with a pH sensitive polymer to prepare the pH / ROS dual-response carrier-free nano-particles. The sophocarpidine-kaempferol carrier-free nanoparticles with pH / ROS dual response, prepared by the invention, have the advantages of good targeting property, high biocompatibility, small toxicity and adverse reaction and the like, meanwhile, the nanoparticles have a good anti-inflammatory effect, and a new choice is provided for the treatment of ulcerative colitis.

The invention belongs to the technical field of biological medicine, and particularly discloses application of kaempferol in preparation of a medicine for treating chronic obstructive pulmonary disease. The invention provides application of kaempferol in preparation of a medicine for treating chronic obstructive pulmonary disease. The invention provides a medicine for treating chronic obstructive pulmonary disease. The medicine comprises kaempferol or pharmaceutically acceptable salt thereof as an active ingredient. The invention aims to provide the application of kaempferol in preparation of the medicine for treating the chronic obstructive pulmonary disease, and the chronic obstructive pulmonary disease is treated by adjusting an AMPK signal channel, a Hippo signal channel, an MAPK signal channel and a PI3K-AKT signal channel.

The invention discloses application of kaempferol in promotion of gene editing, and belongs to the field of biological medicine. The application specifically comprises kaempferol as an accelerant for CRISPR (clustered regularly interspaced short palindromic repeats) gene editing and application of kaempferol in preparation of non-viral site-specific integrated CAR T cells. The invention finds that the kaempferol can significantly increase the CRISPR gene editing efficiency, and cell experiments find that the kaempferol has a significant promotion effect on the positive rate of CAR T cells after electrotransfection, and the tumor killing function of the T cells is greatly improved.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention discloses an on-line extraction self-optimization method for acanthopanax sessiliflorus flavone based on reinforcement learning. According to the method, multi-source data in the extraction process is collected in real time, and a fusion feature vector is generated after preprocessing; establishing a mapping relation from the feature vector to the contents of rutin, quercetin, kaempferol and hyperoside by using an offline test sample, and constructing a virtual measurer capable of outputting a target flavone composition vector and uncertainty thereof; constructing a safety domain boundary according to a solvent flash point temperature, an equipment tolerance temperature upper limit and a component thermal degradation temperature threshold, and calculating a time-varying safety constraint threshold in combination with uncertainty; performing linkage optimization on the temperature, the ethanol volume fraction, the solid-to-liquid ratio, the soaking time and the circulating flow by adopting a reinforcement learning strategy, and comprehensively considering flavone composition deviation and solvent energy consumption by a reward function; and finally, outputting the optimized parameters to extraction equipment for execution. According to the method, online, lossless and self-adaptive process optimization control is realized, inter-batch fluctuation is remarkably reduced, and the resource utilization efficiency is improved.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The application discloses an application of kaempferol in promoting gene editing and belongs to the field of biological medicines. The application specifically comprises kaempferol as a promoter of CRISPR gene editing and the application of kaempferol in preparing non-virus site-specific integration CAR T cells. It is found that kaempferol can significantly increase the efficiency of CRISPR gene editing, and it is found through cell experiments that kaempferol has a significant promoting effect on the positive rate of CAR T cells after electrotransformation, and greatly improves the tumor killing function of T cells.

The invention belongs to the technical field of traditional Chinese medicine preparation processing, and particularly relates to a safflower extract, safflower formula granules and a method for preparing the safflower extract and the safflower formula granules based on a pure electric drive evaporation low-temperature concentration technology. According to the preparation method of the safflower extract and the safflower formula granules, the safflower is extracted by adding water, and a pure electroconcentration technology is adopted, so that the content and the transfer rate of hydroxysafflor yellow A and kaempferol + 6-methoxy kaempferol in the safflower formula granules can be remarkably increased, the concentration temperature is low, the time is short, and the energy consumption is low.

The present invention relates to the field of medical technology, and more particularly to a method for constructing a characteristic spectrum for detoxification capsules. The present invention improves existing detoxification capsule quality standards by enabling simultaneous detection of chebulic acid, syringin, kaempferol-30-rutinoside, β-asarone, and asaraldehyde. Furthermore, the components of the formulation, such as chebulic acid, syringin, kaempferol-30-rutinoside, β-asarone, and asaraldehyde, are controlled, further ensuring the safety, uniformity, stability, and controllability of the product quality.

The application relates to kaempferol cerium nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines. The kaempferol cerium nanoparticles are prepared by performing coordination co-assembly on kaempferol and cerium ions in the presence of polyethylene glycol at a specific molar ratio, and the nanoparticles have uniform particle sizes and good dispersity. The nanoparticles have the anti-inflammatory activity of kaempferol and the ROS scavenging capacity of cerium ions, and show a significant synergistic effect in in-vivo and in-vitro experiments, can effectively reduce lung inflammation, inhibit oxidative stress, improve lung function, and have good biological safety, thereby providing a new drug candidate scheme for the treatment of ARDS.

The invention discloses a connecting peptide and application thereof in promoting compound glucose methylation modification. A glycosyl transferase-methyltransferase fusion protein gene and genes LtLasS1 and LtLasS2 coded by benzenediol lactone synthetase, which are connected in series by connecting peptides, are co-expressed, and liquid phase and mass spectrometry of a fermentation product are carried out to determine that the fusion protein can realize glucose methylation modification by hydroquinone lactone. Wherein the conversion efficiency of the fusion protein hydroquinone lactone using the DL-4 connecting peptide is the highest, and the relative conversion rate is 65.54 + / -3.75%. Besides, by feeding a substrate kaempferol and testing the glucose methylation conversion efficiency of the artificial fusion protein constructed by the connecting peptide, the conversion efficiency of the fusion protein using the DL-4 connecting peptide to the substrate is 6 times of the conversion efficiency of the fusion protein using a single glycosyl transferase and a single methyltransferase to the substrate, and the relative conversion rate is increased to 60.12 + / -4.79%.

The invention discloses a longan leaf granule which is prepared by the following steps: adding water into longan leaves, heating, extracting, filtering, concentrating, drying, crushing to obtain dry paste powder, adding a diluent into the dry paste powder, uniformly mixing, adding a wetting agent, granulating, and drying to obtain the longan leaf granule. The forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the granules all meet the requirements, and the granules are good in stability, suitable for industrial batch production, capable of treating diabetes, capable of meeting the requirements of doctors for treatment based on syndrome differentiation and increase and decrease along with symptoms and convenient to carry and take by patients. Thin-layer chromatography is adopted for qualitative identification of the longan leaf granules, high performance liquid chromatography is adopted for content determination of quercetin, quercitrin and kaempferol in the longan leaf granules, operation is easy, convenient and accurate, repeatability is good, and a scientific basis is provided for quality evaluation and control of the longan leaf granules.

The invention discloses a copper ion-kaempferol nano-composite and application thereof in treatment of inflammatory bowel disease, the obtained nano-composite has very strong stability and active oxygen scavenging ability, and has excellent oxidation resistance and anti-inflammatory ability, and the specific performance is as follows: the surface is negatively charged, and the nano-composite can specifically target the diseased region of intestinal inflammation, so that the copper ion-kaempferol nano-composite can be applied to treatment of inflammatory bowel disease. The intestinal mucosal barrier injury is effectively repaired, the imbalance state of intestinal flora is adjusted, and the intestinal inflammation microenvironment is remarkably improved; the antioxidant and anti-inflammatory capacities of cells can be enhanced by regulating and controlling a PI3K-Akt signal channel and a nuclear factor kappa B (NF-kappa B) signal channel; the compound shows an excellent treatment effect in mouse models of typical inflammatory bowel diseases such as ulcerative colitis and Crohn's disease and related complications of bowel diseases. The invention provides a new way for constructing a multi-target and multi-layer treatment platform for the inflammatory bowel disease of a natural product.

The invention discloses a method for rapidly detecting the content of aluminum ions in raw wine. Kaempferol is used as a probe, the detection response is rapid, fluorescence signal reading is completed within 2 min, the method is far better than a traditional method, and the method is suitable for on-site rapid detection; the method is high in selectivity, free of interference of common metal ions in raw wine and good in linear relation.

The invention provides a kaempferol nano drug delivery system which comprises PLGA (poly (lactic-co-glycolic acid)), kaempferol and mannose, a drug is entrapped by the PLGA to form PLGA microspheres, and the surfaces of the PLGA microspheres are coupled with mannose nano-particles with a targeting function through an amide reaction; the invention protects the application of the kaempferol nano drug delivery system in rheumatoid arthritis. The kaempferol nano drug delivery system is a nano drug delivery system with a mannose receptor targeting characteristic. The invention relates to the field of targeted drugs, in particular to a kaempferol nano drug loading system which is a drug loading system for treating rheumatoid arthritis. The drug loading system for treating rheumatoid arthritis provided by the invention not only has higher encapsulation efficiency and drug loading capacity, but also is lower in preparation cost, also has excellent biocompatibility and biological safety, and has a more excellent anti-rheumatoid arthritis effect.

The invention discloses application of kaempferol in repairing imbalance of fish intestinal microflora. Researches show that exposure of tetramethyl bisphenol A (TMBPA) affects richness and diversity of fish enteric microorganisms, the richness and diversity of the fish enteric microorganisms are dynamically changed during exposure, relative proportion of harmful bacteria in the fish enteric microorganisms is increased, and unbalance of the fish enteric microorganisms is caused; by adopting kaempferol administration treatment, the proportion of harmful bacteria in the intestinal tracts of the fishes can be reduced, the proportion of beneficial bacteria in the intestinal tracts of the fishes can be increased, and unbalanced intestinal microflora of the fishes can be helped to recover to a healthy state. The result shows that kaempferol can repair fish intestinal microflora imbalance caused by TMBPA and restore fish intestinal health.

The invention discloses a method for simultaneously determining the contents of baohuoside, mangiferin, kaempferol, timosaponin A III and timosaponin B II in rhizoma anemarrhenae based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. The method is based on an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, a methanol organic phase-0.1% formic acid water phase system is used as a mobile phase, a Waters Acquity UPLCBEH C18 column is selected, ESI is used as a mass spectrometry detection ion source, and the contents of the five traditional Chinese medicines in the rhizoma anemarrhenae powder are accurately quantified at the same time in a positive and negative ion synchronous scanning and multi-reaction monitoring mode. The method has the advantages of simple pretreatment, high detection sensitivity and good reproducibility, and can be used for identification or quality control of main components of corresponding traditional Chinese medicines.

The application belongs to the technical field of chemical analysis and detection, and discloses a zinc oxide quantum dot fluorescent sensor and application of the zinc oxide quantum dot fluorescent sensor in detection of kaempferol. The zinc oxide quantum dot fluorescent sensor is prepared by stirring zinc salt solution and lye uniformly, then adding amino silane and water to carry out sol-gel reaction to prepare a ZnO-QDs fluorescent probe solution; then, a substrate is immersed in the ZnO-QDs fluorescent probe solution to carry out incubation, and drying is carried out to obtain the zinc oxide quantum dot fluorescent sensor. S The zinc oxide quantum dot fluorescent sensor is applied to detection of kaempferol, can produce specific response with kaempferol, makes the fluorescence intensity of the ZnO-QD fluorescent probe gradually increase with the increase of the concentration of kaempferol, and can effectively exclude the interference of structural analogs such as quercetin and luteolin, so that the kaempferol in the sample can be accurately quantified; and the detection cost is low, the operation is convenient, the detection efficiency is high, the detection is green and safe, and the zinc oxide quantum dot fluorescent sensor is suitable for popularization and application.

The invention relates to the technical field of hair supplies, in particular to plant-extracted anti-hair-loss hair-growing liquid and a preparation method thereof. The plant extraction anti-hair loss hair growth liquid provided by the invention is prepared from hair growth factors, peppermint oil, isohexylene glycol, 1, 3-butanediol and water, the hair growth factor is obtained by extracting polygonum multiflorum, eclipta, white mulberry root-bark, rhizoma kaempferiae and fructus chebulae. The hair growth liquid obtained by the invention has the advantages of low irritation, moistening and transdermal effects, good compliance effect for a user and efficient hair loss prevention and hair growth effects, and hair growth active factors can be efficiently delivered and effectively play the hair loss prevention and hair growth effects.

The application relates to the field of medicine, in particular to a ROS (reactive oxygen species)-responsive liposome containing kaempferol and a preparation method and application thereof, to solve the problems of poor kaempferol efficacy and how to reduce the toxicity of PFOB in the prior art, realize stable kaempferol drug release, and improve the bioavailability. The liposome is a ROS-responsive liposome containing kaempferol, the liposome is a DTP liposome, the DTP liposome is a double-layered inner and outer layer with distearoyl phosphatidyl ethanolamine-polyethylene glycol as the outer shell of the liposome, a ketone thioacetal bond is located between the outer shells of the liposome, the drug loaded in the DTP liposome is a PEG-PFOB-PEG-kaempferol combination, namely a DTP@PPPK liposome, and the DTP@PPPK liposome can fully exert the efficacy and improve the safety.

The application belongs to the field of Chinese herbal medicines, and particularly relates to a method for preparing acylated flavonoid glycoside B (kaempferol-3-O-[6'''-O-E-p-coumaroyl-beta-D-glucosyl-(1->2)-alpha-L-rhamnose glycoside]) from ginkgo leaf extract. The method first uses macroporous adsorption resin to preliminarily purify the ginkgo leaf extract, and then uses activated white clay to decolorize the ginkgo leaf extract. After that, the decolorized flow fraction is separated by using flash medium-pressure preparative chromatography and silica gel column chromatography, and a large amount of acylated flavonoid glycoside B is successfully prepared. The purity of the separated acylated flavonoid glycoside B reaches UV >= 98%, ELSD >= 98%, and the recovery rate is high, wherein the acylated flavonoid glycoside B is 75%. The method has the advantages of large preparation amount, high stability, strong repeatability, simple process steps, high purity of the separated monomeric compound, and the like, and is an ideal separation process, which lays a foundation for subsequent industrial scale production.

The invention relates to the technical field of traditional Chinese medicines, and discloses a classic prescription for treating hypoovarian reserve function, which is prepared from the following raw materials: 30 to 40g of Chinese yam, 20 to 30g of semen cuscutae, 10 to 20g of fructus lycii, 10 to 15g of radix achyranthis bidentatae, 10 to 20g of codonopsis pilosula, 10 to 20g of poria cocos, 20 to 30g of malt, 10 to 15g of angelica sinensis and 10 to 30g of radix puerariae. The classical prescription for treating the hypoovarian reserve function has a positive curative effect, and modern network pharmacology also scientifically proves that active ingredients such as quercetin, daidzein, kaempferol and the like contained in the kidney-tonifying and menstruation-regulating prescription are flavonoid compounds from the aspect of action mechanism, so that the prescription has very good anti-inflammatory and anti-oxidative stress ovarian protection effects; the application range is wide, clinical manifestations of patients with the ovarian reserve function decline are generally hypomenorrhea, menoxenia, amenorrhea, infertility or perimenopausal symptoms, and the kidney tonifying and menstruation regulating prescription has a certain regulating effect on the hypomenorrhea.

The present invention discloses a biological polypeptide detection method, which belongs to the field of biological detection technology and comprises the following steps: taking muscle tissue of a sample, crushing it to obtain a homogenous sample for standby use; adding water to the homogenous sample, and after crude extraction, adding an adsorption resin, after static adsorption, filtering and collecting the adsorption resin, and using ethanol desorption treatment, filtering out the adsorption resin, and using the obtained filtrate as a sample solution for standby use; using UHPLC-MS / MS coupling method to detect the content of carnosine and anserine in the sample solution; wherein the adsorption resin is a polystyrene-divinylbenzene copolymer macroporous adsorption resin or a modified macroporous adsorption resin I, and the modified macroporous adsorption resin I adopts (-)-catechin and kaempferol-3-O-sophorobiose-7-O-glucoside to modify the polystyrene-divinylbenzene copolymer. The method of the present invention is simple to operate, has high accuracy and good reproducibility, and can improve the extraction effect of carnosine and anserine.

The present application relates to the technical field of health food, and particularly relates to a composition and application thereof in maintaining blood glucose health level. The composition comprises rutin, quercetin, kaempferol and pectin. Experiments show that the composition of the present application significantly reduces the activity of alpha-glucosidase, significantly delays starch digestibility, thereby reduces the postprandial blood glucose level of mice, and the effect is obviously better than that of other flavonoid and pectin compositions. It is shown that the rutin, quercetin, kaempferol and pectin are compounded according to a certain proportion, and obvious synergistic effect is produced. In addition, the composition prepared by the present application reduces the use amount of quercetin, increases the utilization rate of pectin, reduces the production cost, and the raw materials are pure natural, safe and non-toxic, functional, low in cost, and have wide application prospect in the deep processing of starch-based health food.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

This invention provides an extract of *Hedyotis diffusa*, its preparation method, and its uses. When the extract is measured under reversed-phase high-performance liquid chromatography, the peak area A of 6''-malonyl *Hedyotis diffusa* I is... M Peak area A with cypermethrin I B The ratio is less than 0.1, and the peak area A of free kaempferol is... K With A B The ratio shall not exceed 0.15. The preparation method includes: adjusting the pH of the aqueous extract of *Kaempferia galanga* to 11-13.5, and performing alkaline hydrolysis at 25-65°C for 20-60 minutes. This invention utilizes the difference in stability of malonyl bonds and glycosidic bonds under alkaline conditions to achieve the selective conversion of 6''-malonylkaempferol I to kaempferol I, increasing the content of kaempferol I while maintaining the integrity of the glycosidic bonds and inhibiting or controlling the formation of free kaempferol. The resulting extract can be used to prepare anti-inflammatory and antioxidant drugs or health foods.

The present invention relates to a use of a moiety selected from the group consisting of: (a) a nucleic acid encoding shRNA or siRNA specific for NR4A1 comprising the sequence of SEQ ID NO: 5 or SEQ ID NO: 6; (b) a NR4A1 antagonist selected from the group consisting of 1,1- bis(3'-indolyl)-1-(p-hydroxyphenyl)methane (DIM-C-pPhOH), resveratrol, a 3-chloro-5- methoxy analog (DIM-C-pPhOH-3-Cl-5-OCH3) and a flavonoid selected from the group consisting of kaempferol and quercetin; (c) a vector comprising a nucleic acid molecule encoding menin; and (d) a NF-ĸB inhibitor, in the manufacture of a medicament for treating pancreatic neuroendocrine tumors (PNETs) in a subject, wherein the medicament is to be administered to the subject, thereby reducing cellular proliferation of PNETs. The present invention also relates to a method of treating pancreatic neuroendocrine tumors (PNETs) in a subject.