157 results about "Free Radical Scavenging Activity" patented technology

The present invention concerns metal oxide semiconductor nanoparticles with free radical scavenging activity, compositions comprising such nanoparticles, methods for their use, and methods for their production. In one aspect, the invention concerns a method for enhancing the survival or viability of transplanted cells, comprising administering an effective amount of metal oxide semiconductor nanoparticles to a target anatomical site of a subject before, during, or after administration of transplant cells to the subject. Preferably, the metal oxide nanoparticle is a cerium oxide (ceria) nanoparticle.

The invention discloses a method of extracting flavonoid glycosides from grosvenor monordica leaves and a product thereof. The method comprises the following steps: taking Grosvenor monordica leaves, boiling the Grosvenor monordica leaves with water, carrying out extraction and filtration twice to three times, each extraction lasting 50 to 100 min, combining filtrates, carrying out adsorption on an obtained filtrate by using a macroporous resin, rinsing the resin with water until effluent is colorless, carrying out elution by using ethanol or absolute methanol, collecting eluent, removing ethanol or methanol, placing the eluent in a refrigerator for 20 to 30 h, allowing precipitate to deposit, dissolving the precipitate with absolute ethanol, carrying out filtration and evaporating an obtained filtrate so as to obtain a crude extract, kaempferol-3,7-a-L-dirhamnoside. The extraction method provided in the invention is simple, scientific and reasonable, has strong feasibility and enables an obtained extract product to have a high purity, wherein, the purity of a refined extract product is higher than 95%; the extract product obtained in the invention has good free radical scavenging activity and a wide application prospect. In addition, application of the method enables full utilization of considerable waste grosvenor momordica leaves to be realized, thereby improving the utilization rate of natural resources and reducing waste.

The present invention relates to emblic extract and its preparation process and application. Dried emblic powder is first microwave assisted solvent extracted, and the emblic extract is then extracted with diethyl ether and ethyl acetate and macroporous resin purified to obtain purified emblic extract. The purified emblic extract is one kind of natural antioxidant with emblic polyphenol in the content over 93 % as the main effective component. The emblic extract as natural antioxidant is superior to available synthetic antioxidants, and has high DPPH free radical eliminating activity, high lipid peroxide inhibiting activity and high peroxidation resisting capacity.

The invention discloses a method for preparing oligomeric procyanidins from lychee extract. Lychee contains a lot of procyanidins, and the procyanidins are provided with strong antioxidant activity and free radical scavenging activity. No extraction method for oligomeric procyanidins has been found until now. The method is characterized in that the procyanidins in the lychee extract are specifically hydrolyzed through a method of a first extraction, a second centrifugation, a third enrichment, a fourth separation and purification, a fifth enrichment by polyphenol eluents, a sixth drying and a seventh hydrolysis by organic acids, reducing the polymerization of the procyanidins, greatly increasing the content of monomeric and oligomeric procyanidins in the lychee extract, and making the natural resources of the procyanidins in the lychee extract more fully utilized. After the hydrolysis of the organic acids, polyphenol content in the dry powder of the extract is more than 70%, the average degree of polymerization of the procyanidins is less than 2.0, the content of the procyanidin monomer epicatechin is more than 6.5%, the content of the procyanidin oligomer A1 is more than 5.0%, and the content of the procyanidin oligomer A2 is more than 4.0%.

The invention discloses a natural plant composition with a skin whitening effect and application thereof to cosmetics. The natural plant composition is prepared from, by mass, 0.01-20% of white mulberry root bark extract, 0.01-20% of rhizoma atractylodis extract, 0.01-20% of radix paeoniae alba extract, 0.01-20% of ginger root extract, 0.01-20% of Chinese yam extract and the balance water. According to the plant composition, efficient tyrosinase inhibiting activity of the white mulberry root bark extract, the rhizoma atractylodis extract and the radix paeoniae alba extract, excellent oxidation resistance and free radical scavenging activity of the ginger root extract and excellent moisture retention performance of the Chinese yam extract are combined organically, formation of melanin of the skin base layer is effectively reduced, the skin can be fairer, clearer and brighter, and the effect of whitening the base is achieved.

The invention discloses pseudopeptides with a formula as the following. The invention further discloses a preparation method and an application of the pseudopeptides. The pseudopeptides provided by the invention have excellent thrombolytic activities, antithrombotic activities, anti-inflammatory activities, OH free radical scavenging activities and nanostructures.

The invention discloses a Chinese mahonia extracting solution as well as a preparation method and an application thereof. The Chinese mahonia extracting solution prepared by water extraction has good free radical scavenging activity and the effect of inhibiting peroxidation of lipoxygenase and lipid, and due to the characteristics, the Chinese mahonia extracting solution has the effects of scavenging excessive free radicals accumulated in bodies, preventing formation of lipofuscin, protecting cell membranes, maintaining the normal metabolism of cells and prolonging organism senescence; when being applied to cosmetics, the Chinese mahonia extracting solution has remarkable effects of improving facial laxity and reducing generation of wrinkles and black spots.

The invention discloses a preparation method of rosemary extracts. According to the method, plant rosemary are used as raw materials, and the rosemary extracts are prepared under certain process conditions. The extracts comprise various antioxidant ingredients including carnosic acid, carnosol, rosmanol, ursolic acid, rosmarinic acid and the like according to certain proportion, the antioxidant activity of the rosemary extracts is measured by an iodine quantity colorimetric method, the.OH free radical clearing activity of the extracts is researched through the electron paramagnetic resonance technology, and the obvious antioxidant effect is discovered. The invention also discloses the rosemary extracts prepared by using the method and an application of the rosemary extracts in the preparation of food additive antioxidants.

The invention discloses a polyphenol compound in tobaccos and a preparation method and application thereof. The compound has a novel structure of the general formula (I). The compound is prepared is prepared by the following steps of: crushing a tobacco sample, and then performing ultrasonic extraction on the powder for three times by using 70 percent ethanol; combining extracted solution and concentrating the mixture into an extractum; primarily separating the extractum by using silica gel column chromatography; and further separating the extractum by adopting a high-efficiency liquid phase semi-preparation chromatogram to obtain the needed new compound. The compound is treated by antioxidant activity and free radical removal activity screening, and experimental results show that the compound shows stronger antioxidant activity and free radical removal activity.

The invention relates to a new coumarin active content, i.e. pigeon pea lactone extracted and separated from cajan leaves, an extraction and separation method and an application thereof. The structure of the compound is shown as a figure 1. The invention adopts the technical scheme that agricultural waste of cajan leaves is used as raw material which is extracted by a solvent; the extracting solution is concentrated and dispersed in water; an ultrasonic oscillation flocculation technology, a bubble column extraction technology and a column chromatography separation technology in positive-phase silica gel are adopted and combined with a low-temperature crystallization and recrystallization technology to obtain the content with the purity higher than 95 percent; and the anti-oxidation activity and the action of the chemical content on cardiovascular diseases are detected by a DPPH model, a beta-renieratene-linoleic acid model, an in-texture SOD enzyme model, a platelet aggregation model induced by ADP in corpora and an aorta systole model induced by phenylephrine. Results show that the active content has favorable free radical scavenging activity, anti-oxidation activity in corpora and in vitro, platelet aggregation restraining action in corpora and a certain hemangiectasis action. Meanwhile, the preparation method of the pigeon pea lactone in the invention is easy and practical and can obtain a product with high purity high yield and high added value in a short period which is suitable for industrialized production and application, can be further developed and researched into a new antioxidant as an additive for medicines, foods and cosmetics.

The invention discloses curcumin nano-particles and a preparation method thereof. The curcumin nano-particles compounded by BSG protein extract and lecithin are prepared with a base and/or alcohol extracted BSG protein extract as carrier of curcumin. The prepared curcumin nano-particles are spherical and uniform, and have good dispersibility, narrow PDI value and high encapsulating efficiency. Thecurcumin is wrapped in a hydraophobic nuclear of the BSG protein extract, and solubility, heat stability and ultraviolet radiation stability of the wrapped curcumin are remarkably improved; and besides, the wrapped curcumin has higher DPPH free radical scavenging activity than free curcumin.

The invention discloses an isobenzofuran compound as well as a preparation method and application thereof. The isobenzofuran compound is obtained by separating from a traditional Chinese herbal medicine radix puerariae; the compound is named as 5-methoxy-2,2-dimethyl-2H-furo[3,4-g] chromen-8(6H)-one as an English name, and a molecular formula of the compound is C14H14O4; a structural formula of the compound is shown in the description. The preparation method of the isobenzofuran compound comprises the following steps: using the traditional Chinese herbal medicine radix puerariae as a raw material and carrying out extractum extraction, organic solvent extraction, MCI decolorizing, silica-gel column chromatography and high pressure liquid chromatography separation to obtain a finished product; the isobenzofuran compound is subjected to antioxidant activity and free radical removal activity screening, so the isobenzofuran compound has higher antioxidant and free radical removing activities; the compound is used for producing cigarettes and can be used for effectively removing the free radicals in smoke gas of the cigarettes.

The invention discloses a method used for producing black-bone silky fowl soybean sauce via fermentation, and belongs to the technical field of food processing. According to the method, black-bone silky fowl, soybean meal or soybean, and wheat are taken as the main raw materials, multi-strain koji-making and conventional fermentation technology are combined to produce the black-bone silky fowl soybean sauce. The obtained black-bone silky fowl soybean sauce is mellow and thick in mouthfeel, fresh and delicious; agreeable aftertaste lasts long; sauce flavor is strong; amino acid nitrogen content and total nitrogen content are 11 to 18% higher and 6 to 12% higher than that of commercially available first-grade standard soybean source respectively; and oxidation resistance (reducing power determination, DPPH free radical scavenging activity, and metal chelating ability) and dark matter content are both higher than that of commercially available first-grade standard soybean source. Compared with conventional soybean source, the black-bone silky fowl soybean sauce possesses a distinct characteristic flavour, excellent oxidation resistance, and high additional value.

The invention provides a method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at the same time and a method for separating and purifying two kinds of known flavonoid constituents at the same time. Through an HSCCC method, four kinds of flavonoid constituents are successfully separated and purified from the meconopsis integrifolia flowers; the separation speed is high, and the method lays a foundation for material basis research of the meconopsis integrifolia flowers. The research finds that the separated known compounds or new compounds have good free radical scavenging activity and can be used as antioxidant medicine or a health-care product.

The invention discloses a Hainan green plum stem extractive with a function of scavenging free radicals and a preparation method thereof. The preparation method comprises the following steps: adding an organic solvent to Hainan green plum stem drying powder, and extracting; filtering an extracting solution, concentrating the filtered extracting solution under reduced pressure so as to obtain total extract, adding water, suspending, extracting sequentially by using petroleum ether and ethyl acetate, recovering under reduced pressure so as to respectively obtain a petroleum ether part and an ethyl acetate part, carrying out gradient elution on the obtained ethyl acetate part by using a chloroform and methyl alcohol mixed solvent system, wherein the chloroform and methyl alcohol component with the volume ratio of chloroform to methyl alcohol being 100: 30 has excellent free radical scavenging activity and comprises 16 resveratrol oligomers compounds. The Hainan green plum stem extractive prepared by the method is high in yield, simple in technological process, low in cost and suitable for large-scale preparation; and the obtained extractive has excellent free radical scavenging activity and can be applied to healthcare food, medicines and food with functions of resisting oxidation and scavenging free radicals.

The invention relates to an isobenzofuran compound with an effect of scavenging free radicals, and a preparation method and an application of the isobenzofuran compound, and belongs to the technical field of phytochemistry. The isobenzofuran compound is separated from tobaccos; the compound is named 6,8-dihydro-2,2-dimethyl-2H-furan [3,4-g] chromene, the English name is 6,8-dihydro-2,2-dimethyl-2H-furo[3,4-g] chromene, the molecular formula is C13H14H2O, and the structure is as shown a formula (1) in the description. The preparation method comprises the steps: with tobaccos as the raw material, extractum extraction, organic solvent extraction, MCI decoloration, slica column chromatography and high-performance liquid chromatographic separation. Antioxidant activity and free radical-scavenging activity screening is carried out on the compound, so that the experiment result shows that the compound has relatively high antioxidant activity and free radical-scavenging activity.

The invention relates to the technical field of microbial fermentation, and discloses application of saccharomyces cerevisiae ZLG-6 and lactobacillus plantarum Picp-2 in preparation of a blueberry fermentation microbial agent or blueberry fermentation. Specific screened saccharomycetes and lactic acid bacteria are selected to act on blueberry fermentation, and the prepared fermented raw material has high DPPH free radical clearing activity, higher anthocyanin content, higher polyphenol content and higher SOD enzyme activity, and can serve as an effective component of cosmetics.

The invention discloses an anthraquinone compound, and a preparation method and application thereof. The anthraquinone compound is separated from aloe; the molecular formula of the anthraquinone compound is C18H16O5; the structural formula of the anthraquinone compound is as described in the specification; and the anthraquinone compound is named as 3-hydroxy-6-hydroxyethyl-5-methoxy-2-methyl-anthraquinone. The anthraquinone compound is prepared from aloe through extraction of extract, column chromatography on silica gel and high-pressure liquid chromatographic separation. Experimental results show that the compound has good antioxidation activity and free-radical removal activity; and when applied as an anti-oxidant for flavoring essential oil of a cigarette, the compound can effectively inhibit oxidation deterioration of the flavoring essential oil and substantially prolonging the shelf life of the cigarette without any influence to the smoking quality of the cigarette.

The invention discloses a preparation method of blueberry leaf polyphenol having anti-oxidation activity. The method comprises the steps of ultrasonic extraction, filtration, centrifugation, taking of the obtained supernatant, vacuum concentration and HPD 400 type macro-porous resin column chromatographic purification. The free radical scavenging activities of crude blueberry leaf polyphenol extract and the blueberry leaf polyphenol purified through the HPD 400 type macro-porous resin purification are evaluated through an ABTS technology and a DPPH technology, and rutin us as a reference substance. The preparation method of the blueberry leaf polyphenol has the characteristics of high extraction efficiency, good repeatability, mild conditions and no pollution, and the prepared blueberry leaf polyphenol has significant anti-oxidation activity, so the blueberry leaf polyphenol has a wide application prospect in the food industry and the pharmaceutical industry.

The invention provides laminarin and an extraction and separation method thereof. The method comprises the following steps: extracting a laminarin crude extract having a yield of 3.6 percent, which is superior to that of a traditional water extraction method, by adopting a high-temperature high-pressure water extraction method; performing two-step separation by DEAE-anion exchange chromatography and Sephacryl S-300 gel chromatography to obtain a kelp purified polysaccharide PS-3-1 having a molecular weight of 132 KDa. The prepared kelp purified polysaccharide has free radical scavenging activity, has a scavenging rate for hydroxyl radicals and superoxide anion being over 70 percent, and can be developed into a free radical scavenging drug. Furthermore, the purified polysaccharide has a reproduction inhibiting activity on vascular smooth muscle cells, has a reproduction inhibition rate of over 75 percent, and can be used for preparing a vascular smooth muscle cell reproduction inhibitor medicine.

The invention relates to sesame peptide powder, and a preparation method and application thereof. The peptide content of the sesame peptide powder is 90wt% or above, wherein the molecular weight of more than 90% of sesame peptide is less than 1000 Dalton. Degreased sesame meal is subjected to protein extraction and enzymolysis, purified, concentrated and dried to obtain the sesame peptide. The sesame peptide powder has good free radical removing activity, improves the sleep quality, enhances human immunity and meanwhile has an anti-haze effect. The preparation method is easy to operate, high in yield, low in cost, stable in product quality, environmentally friendly in process and suitable for large-scale production.