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South American wedelia chinensis antioxidant extract as well as preparation method and application of extract

An anti-oxidation and extract technology, which is applied in the field of anti-oxidation extract of Wedelia chrysanthemum flower and its preparation, can solve the problems of separation and identification of chemical components of effective anti-oxidation parts of Wedelia chrysanthemum flower, and achieve low cost, excellent anti-oxidation The effect of oxidation activity and simple process flow

Active Publication Date: 2018-03-06
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no patents related to the anti-oxidation application of Wedelia chinensis, and there is no report on the separation and identification of effective antioxidant parts and further chemical components of Wedelia chinensis.

Method used

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  • South American wedelia chinensis antioxidant extract as well as preparation method and application of extract
  • South American wedelia chinensis antioxidant extract as well as preparation method and application of extract
  • South American wedelia chinensis antioxidant extract as well as preparation method and application of extract

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] The preparation method step of South American Wedelia chrysanthemum extract is as follows:

[0027] 1) Add 85-95% ethanol or methanol aqueous solution to the pulverized Wedelia chrysanthemum medicinal material (passed through a 20-80 mesh sieve), soak at room temperature, heat or ultrasonically extract, the extraction time is 60 minutes to 7 days, and the solid-liquid ratio 1:1~1:10;

[0028] 2) Filtrate the extract of Wedelia chrysanthemum medicinal materials, recover under reduced pressure to obtain the total extract, add water to suspend, sequentially extract with petroleum ether and ethyl acetate, recover under reduced pressure to obtain the petroleum ether part, ethyl acetate part and water-soluble part respectively ;

[0029] 3) Among them, the ethyl acetate part is chromatographed on a silica gel column, first eluted with chloroform:ethyl acetate (1:0-10:1) to remove petroleum ether extraction residues and small polar oil components, and then eluted with acetone...

example 1

[0032] The preparation of example one Wedelia chrysanthemum flower effective fraction

[0033] South America Wedelia chrysanthemum (10Kg), at room temperature adopts 95% ethanol at room temperature to soak 3 times, 7 days / time, combine extracts, concentrate under reduced pressure to obtain total extract (1.2Kg), add water to suspend and disperse, respectively use sherwood oil, chloroform, Ethyl acetate and n-butanol were extracted to obtain petroleum ether part (200g), ethyl acetate part (380g) and water soluble part (560g).

[0034] The active site after partial degreasing of ethyl acetate was subjected to repeated silica gel and Sephdex LH-20 column chromatography to obtain 9 flavonoids and 4 phenylpropanoids, which were respectively petroleum ether: acetone (20:1), chloroform:acetone (50:1) silica gel column chromatography, Sephadex LH-20 column chromatography (chloroform:methanol=1:1, v / v) to obtain compounds 4 and 2 respectively ',4'-Trihydroxy-3-methoxychalcone (2) 7 mg...

Embodiment 2

[0036] Example 2 Study on Antioxidant Activity of Wedelia chrysanthemum Flower Extract

[0037] 1. Wedelia chrysanthemum flower extract scavenging DPPH free radical activity

[0038] 1.1 Experimental method: 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) was prepared into a 65 μM absolute ethanol solution. Precisely weigh the test samples and dissolve them in absolute ethanol for later use.

[0039] Pipette 50 μL of the test sample and add it to 3 mL of 65 μM DPPH solution, and use an equal amount of absolute ethanol for the blank control, mix well, place it at room temperature for 30 minutes, and then place it in a cuvette to measure the absorbance at 517 nm of the mixed solution of the sample and DPPH. Each absorbance was measured 3 times in parallel, and the average value was taken to calculate the clearance rate of the series of concentrations to obtain the IC 50 value. The clearance rate (Y) is calculated according to the following formula:

[0040] Y=[l-(Ai-Aj) / Ao]×10...

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Abstract

The invention discloses a South American wedelia chinensis antioxidant extract as well as a preparation method and application of extract. The extract is extracted from dry powder of South American wedelia chinensis by adding a solvent; extracting solution is subjected to filtration and reduced pressure concentration to obtain total extract, extraction is carried out with petroleum ether and ethylacetate sequentially, reduced pressure recycling is carried out to obtain a petroleum ether part, an ethyl acetate part and a water dissolving part respectively, the obtained ethyl acetate part and water dissolving part respectively have excellent DPPH free radical scavenging activity and oil oxidation resistant activity, and the ethyl acetate part contains nine fiavonoids and four penylpropanoids. The obtained extract is high in yield, technological process is simple, and the extract is applicable to relatively large-scale preparation. The obtained extract has excellent antioxidant activityand can be applied to antioxidant drugs and food preservation as active components.

Description

technical field [0001] The invention belongs to the field of natural plant extraction, and in particular relates to an anti-oxidation extract of Wedelia chrysanthemum flower, its preparation method and application. Background technique [0002] South American Wedelia (Wedelia trilobata), also known as three-lobed (leaf) Wedelia, is a perennial herbaceous plant of the genus Wedelia in the family Compositae. It is native to central and southern tropical America. The leaves or aerial parts of the plant are used in traditional medicine in the Caribbean and Central America to treat back pain, muscle spasms, rheumatism, stubborn wounds, ulcers, swelling, and joint pain. It was first introduced to Hong Kong in the 1870s to replace the traditional Chinese medicine Wedelia, mainly used to treat diphtheria, whooping cough, dysentery, hemorrhoids, and bruises. Nowadays, on the roadsides, fields, ditches or wet grasslands widely distributed in Guangdong, Guangxi, Fujian and Hainan, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P39/06A23L3/3472
Inventor 陈文豪惠阳陈光英宋小平武晓雪周秀悄
Owner HAINAN NORMAL UNIV
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