109 results about "Phenylpropanoid" patented technology

The present invention provides methods and compositions relating to generating plants having increased levels of oil and protein. This invention further provides recombinant expression cassettes, host cells, transgenic plants and genetically altered plants. The invention further provides isolated polynucleotides and their encoded proteins that are involved in phenylpropanoid biosynthesis.

The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular/modified/synthetic gene(s) of select enzymes are processed and inserted into an expression system (vector, cosmid, BAC, YAC, phage, etc.) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.

The invention discloses an extraction and purification process for medicinal components of Scrophulariaceae, namely an extraction and purification process for iridoid and phenylpropanoid components: pulverizing dry roots of Scrophulariaceae, soaking, water extraction, concentration of the extract, and ethanol precipitation , resin adsorption, ethanol elution, concentrated drying and other procedures obtained. The extraction and purification process of the invention has high extraction rate, can reach more than 50%, stable and controllable quality, simple method and low composition, and is suitable for large-scale industrial production, thereby providing a new way for the development and utilization of Scrophulariaceae.

The invention relates to an application of benzo-phenylpropanoids in preparing drugs for treating viral hepatitis B, in particular to two benzo-phenylpropanoids or application of pharmaceutically acceptable salt thereof in preparing drugs for inhibiting the replication of hepatitis B virus desoxyribonucleic acid (HBV DNA) and treating hepatitis B virus infection diseases. The two benzo-phenylpropanoids definitely inhibit the activity of the HBV DNA, have the replication inhibition activity on the HBV DNA at high dose (100 microgrammes/milliliter) of 1.3-2.2 times higher than the inhibition activity at the highest concentration (10000 units/milliliter) of an alpha-interferon and belong to an efficient non-nucleoside natural product inhibiting the hepatitis B viruses; pharmacodynamics results show the application of the benzo-phenylpropanoids or the pharmaceutically acceptable salt thereof capable of preparing the drugs for inhibiting the replication of the HBV DNA and treating the hepatitis B virus infection diseases in anticipation.

The invention discloses total phenyl propanoid extract and total phenol extract extracted from grassleaved sweetflag rhizome and its preparation method. Total phenyl propanoid extract mainly comprises beta -asaricin, alpha -asaricin, eudesmin, galgravin, veraguensin and other derivant with veraguensin as mother nuclide. Total phenol extract mainly comprises caffeic acid, protocatechuic acid, ferulic acid, vanillic acid and glucosides with vanillic acid as mother nuclide and other derivants. Total phenyl propanoid extract and total phenol extract of grassleaved sweetflag rhizome can be extracted through one or more of methods of solvent-extraction, solvent extraction, precipitation method, macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Total percentage content of phenyl propanoids in the obtained total phenyl propanoid extract of Grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of beta -asaricin and alpha -asaricin occupy 5-100% (w/w).Total percentage content of phenols in total phenol extract of grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of caffeic acid and protocatechuic acid occupy 5-100% (w/w).

Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.

The present invention relates to the preparation of plant cell cultures belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera and their use in cosmetic, nutritional and pharmaceutical fields. In particular, the present invention regards a selected cell culture of meristematic cells of plants belonging to the Dendrobium Phalaenopsis, Anisellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera characterized in that it contains an amount of phenylpropanoids greater than 0.1% expressed on the cell dry weight and an amount of hydrosoluble polysaccharides greater than 5% expressed on the cell dry weight, as well as preparations, pharmaceutical and cosmetic compositions or food supplements containing said cell culture.

Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.

A cell may include heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.

The present invention relates to the field of traditional Chinese medicine pharmacy, particularly to uses of aryl-substituted phenylpropanoid-based compounds in preparation of anti-complement drugs. According to the present invention, the aryl-substituted phenylpropanoid-based compounds 1-3 are extracted from the root of an Aquifoliaceae plant Ilexasprella by using an activity guide separation method, and the results of the classical pathway anti-complement activity evaluation test and the alternative pathway anti-complement activity evaluation test show that the compounds provide strong inhibition effects for the classical pathway and the alternative pathway of the complement system, wherein the inhibition effect on the classical pathway (CH50) is 0.032-0.059 mg/ml, and the inhibition effect on the alternative pathway (AP50) is 0.061-0.275 mg/ml; and the compounds can be used for preparing anti-complement drugs and further preparing drugs for treatment of complement-related diseases.

The invention discloses an anti-hepatitis B virus active phenylpropanoids adamantine ketoxime ester compound. The structural formula of the phenylpropanoids adamantine ketoxime ester compound is shown in the description, and by conducting a HepG2.2.15 cell experiment, we find that the compound has certain inhabitation effect on HBV surface antigen (HBsAg) and e antigen (HBeAg) and is expected to prepare anti-hepatitis B virus medicines.

The invention discloses a method for high-throughput analysis of plant secondary metabolites based on LCMS. A Scheduled-MRM mode is adopted to realize one-needle sample injection within 45min. Meanwhile, 130 types of plant secondary metabolites including anthocyanidin, benzoic acid and derivatives thereof, catechin and derivatives thereof, coumarin and derivatives thereof, dihydrochalcone, flavanone, flavone, flavonol, isoflavone, phenylpropanoids, procyanidine, stilbene, terpenes and the like are subjected to high-throughput absolute quantitative analysis. The method is high in pretreatment extraction efficiency, an instrument analysis method is wide in linear range, and high-abundance and low-abundance secondary metabolites with a large content difference can be detected and analyzed atthe same time; linear correlation is good, and a correlation coefficients are all higher than 0.995; the quantification limit of partial substances is as low as 0.01 ng/mL, and the sensitivity of themethod is high; accuracy is 80.2%-118.2%, and precision is 0.7%-119.9%; and high throughput and method sensitivity can be considered through Scheduled-MRM scanning, and the method can be widely applied to analysis of secondary metabolites in different complex plant samples.

Provided are methods for decreasing carbon flow into lignin in plants, comprising reducing or eliminating, using mutagenesis and/or recombinant means, expression and/or activity of at least one chloroplast-localized arogenate dehydratase (ADT) sufficient to reduce phenylalanine (Phe) availability for metabolism into Phe-derived phenylpropanoids, wherein the amount, level or distribution of lignin is reduced relative to control plants. In particular aspects, the plant has a plurality of chloroplast-localized ADTs, and reducing or eliminating comprises reducing or eliminating expression and/or activity of at least two of the plurality of ADTs. Also provided are recombinant plants or parts or cells thereof, comprising at least one mutation, genetic alteration or transgene that reduces or eliminates the expression and/or activity of at least one chloroplast-localized ADT, wherein the amount, level or distribution of lignin is reduced relative to normal. Further provided are reduced lignin plant products.

The invention provides a dandelion extract as well as a preparation method and application thereof. The dandelion extract is prepared by the following steps: taking fresh dandelion, adding water, squeezing, and filtering to obtain the dandelion extract; wherein the dandelion extract comprises the following components in percentage by weight: 4%-10% of polysaccharide and 0.3%-1% of protein. The dandelion extract disclosed by the invention is rich in macromolecular substances such as polysaccharide, protein and the like, and extremely low in content of small molecular substances such as phenolic acid, flavone, phenylpropanoid and the like, so that the dandelion extract has remarkable antioxidant and anti-inflammatory activity.

The invention discloses an application of Alpinia japonica extract. The Alpinia japonica extract contains total phenylpropanoids of Alpinia japonica and is used for preparation of medicines for reducing uric acid and resisting gout. A research result shows that total phenylpropanoids of Alpinia japonica, a mixture of total phenylpropanoids of Alpinia japonica and total flavonoids of Alpinia japonica, Alpinia japonica alcohol extract and extract of total phenolic acids of Alpinia japonica have very extreme uric acid reduction effects better than those of the known uric acid reduction plant extract and better than that of a positive drug allopurinol, have good effects of protecting renal functions and resisting gout, are safe and effective and have a development and application prospect.

A biocidal composition including one or more of an ester, a phenylpropanoid, a surfactant, a monoterpenoid, an oil, a preservative, and a carrier is described, the composition being capable to act as a biocide for bedbugs, dust mite, spiders, fleas, ticks, mosquitoes, silver fish, and roaches.

The present invention relates to selected and stabilized cell line extracts from Ajuga reptans comprising phenylpropanoids having high antioxidating capacity with a teupolioside titre of between 20% and 90% and a chromophore-free fraction of between 80% and 10%. Said extracts are for use in human and veterinary therapy and for nutritional and cosmetic purposes. Furthermore, the invention relates to a novel phenylpropanoid molecule denominated isoteupolioside.

The invention relates to the technical field of medicines, and discloses an application of phenylpropanoids compounds in preparation of medicines for treating inflammatory diseases. The phenylpropanoid compounds are indicated to be able to inhibit expression effects of inflammatory cytokines TNF-alpha, IL-1beta and IL-6, thereby having anti-inflammatory activity, and providing a direction for development of medicines for treating inflammatory diseases, such as cervicitis, endometritis, pelvic inflammation, mastitis, sphagitis and/or arthritis and other diseases.

The invention discloses application of poplar glycosyl transferase PtGT2 to catalytic synthesis of phenylpropanoid glucosides. The amino acid sequence of the poplar glycosyl transferase PtGT2 is shown in SEQ ID No.2; the phenylpropanoid is sinapinic alcohol, sinapinic aldehyde, coniferyl alcohol, coniferyl aldehyde, sinapinic acid or ferulic acid; and any one of the poplar glycosyl transferase PtGT2 and the phenylpropanoid is put in a reaction tube for enzymatic reaction, and the corresponding phenylpropanoid glucosides can be catalytically synthesized. According to the application disclosed by the invention, a feasible method is provided for synthesizing the phenylpropanoid glucosides efficiently and exclusively by utilizing an enzyme catalysis method; after the application is implemented, large economic benefit is brought for the synthetic industry of the phenylpropanoid glucosides.

The present invention relates to recombinant glycosyl transferase UGT72E3/2 gene having an excellent syringin synthesis ability based on remarkable enzyme specificity for sinapyl alcohol, a method for producing a transgenic plant with increased syringin production based on a metabolic process which uses F5H and CHS genes that are involved with the phenylpropanoid biosynthesis pathway and Myb58 gene as a transcription factor for positive regulation of the gene that is involved with lignin biosynthesis pathway, and a plant obtained by the method. According to the present invention, syringin with various pharmaceutical applications can be effectively produced in a large amount in a plant, and thus it is expected to allow the development of an industry relating to agrobiological materials that are highly valuable as foods or pharmaceuticals.