43 results about "Phenylpropanoid" patented technology

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

A composition having an additive or component having an antimicrobial or a preservative property, and 3-phenylpropanol or a member of the phenylpropanoid family is provided. A composition having a polymer, an organic acid, and 3-phenylpropanol or a member of the phenylpropanoid family is provided as well as a method(s) of using.

The invention discloses a method for improving the self-luminous intensity of a plant, which is characterized in that an AroGL175Q gene or an RgTAL gene and a PtC3H gene or the AroGL175Q gene, the RgTAL gene and the PtC3H gene are expressed in a plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the caffeic acid content, the milk tree alkali content and the luminous intensity are obviously increased is obtained. According to the invention, through metabolic network optimization and transformation, phenylpropane metabolites required by middle and upper streams of a fungus bioluminescence pathway are increased, and synthesis of caffeic acid and milk tree alkali in plants is increased, so that bioluminescence enhancement is realized. The precursor supply bottleneck of a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

The present application belongs to the field of biological genetic engineering, and relates to application of ginseng transcription factor PgMYB14 in improving drought tolerance of plants, wherein a sequence of the PgMYB14 protein is shown as SEQ ID NO. 1, and a sequence of the PgMYB14 gene is shown as SEQ ID NO. 2. The results of the present application show that heterologous expression of the PgMYB14 gene in Arabidopsis thaliana can activate a response pathway to drought and osmotic stress, and up-regulate biosynthesis metabolism of phenylpropanoids, suberin and wax. In addition, PgMYB14 can enhance the drought stress tolerance of Arabidopsis thaliana by removing active oxygen and up-regulating expression of drought stress related genes.

The invention belongs to the technical field of microorganisms, and discloses an amorphophallus bulbifer southern blight infection influence analysis method based on metabonomics and microbiomics. Conjoint analysis shows that core florae (such as Streptomyces, Penicillium and Bauldia) are in significant positive correlation with phenylpropanoids and glutathione (succinic acid, coniferyl alcohol and glutamic acid), succinic acid is in positive correlation with Kribbella and Terrimonas, 2 ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', the 3, 3 '-cycle CMP is negatively correlated with pathogen-related bacteria, which indicates that the metabolite regulates and controls the abundance of specific bacteria through positive / negative feedback so as to drive the microecological balance of rhizosphere, and a collaborative defense strategy that plants recruit beneficial microorganisms and inhibit pathogenic bacteria through metabolism reprogramming is disclosed.

The present invention relates to the use of a mixture of water and an essential oil selected from phenolic, phenylpropanoid and furanocoumarin for separating multilayer composites in order to sort the polymer films or metal films.

The invention discloses a folium cortex eucommiae extract and application of the folium cortex eucommiae extract in prevention and treatment of adriamycin nephropathy, and relates to the technical field of medicines, the folium cortex eucommiae extract is prepared from dried folium cortex eucommiae through water extraction, heating at 65 DEG C, ultrasonic assistance and vacuum freeze drying, LC-MS / MS analysis shows that the folium cortex eucommiae extract contains no less than 200 components, the components mainly comprise flavonoids, terpenes and phenylpropanoids, and the content of the flavonoids, the terpenes and the phenylpropanoids is no less than 100%. The folium cortex eucommiae extract has no obvious cytotoxicity to HK-2 and HBZY-1 cells under the concentration of 0-1500mu g / mL, can be used for preparing medicines for preventing and treating adriamycin nephropathy, inhibits kidney cell apoptosis by down-regulating Bax and up-regulating Bcl-2 and obviously reduces the levels of IL-6, IL-1beta and TNF-alpha in serum, and in an animal model, 150-600mg / kg of the folium cortex eucommiae extract is continuously administrated for 21-30 days in an intragastric manner, so that the effect of preventing and treating adriamycin nephropathy is achieved. The traditional Chinese medicine composition can effectively relieve weight loss, reduce urine protein, serum creatinine and urea nitrogen, increase albumin and improve kidney pathological injury, and is suitable for relieving kidney toxicity in a prophylactic or therapeutic mode in subjects receiving adriamycin chemotherapy.

This invention belongs to the field of modern analytical technology of traditional Chinese medicine, specifically involving a method for detecting chemical components of bitter vine based on UPLC-Q-TOF-MS / MS. This invention is the first to systematically and rapidly perform qualitative analysis on the chemical components of bitter vine, a Dai medicinal herb, identifying a total of 96 compounds, including saponins, phenylpropanoids, flavonoids, terpenes, and organic acids. This provides a scientific basis and data support for elucidating the pharmacodynamic material basis, quality control, and subsequent formulation development of bitter vine.

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The invention relates to a method for purifying total phenylpropanoid from a lonicera macranthoides extract and application of the total phenylpropanoid in medicines. Relates to the technical field of traditional Chinese medicines, in particular to application of lonicera macranthoides total phenylpropanoid (TP) in preparation of medicines with functions of promoting oxidation resistance, reducing blood sugar, inhibiting cholinesterase and resisting inflammation. According to the present invention, a lonicera macranthoides extract is purified by using a macroporous resin, in particular an HPD100 resin, to obtain high-purity TP. The method comprises the following specific steps: (1) loading a sample containing the lonicera macranthoides extract into a macroporous adsorption resin column with the diameter-height ratio of 1: 5 at the flow rate of 1.5-2.5 mL / min; (2) eluting by using deionized water to remove unadsorbed liquid, wherein the dosage is 40-120mL; and (3) carrying out elution by using a 60% alcoholic solution at an elution flow rate of 1.5-2.5 mL / min to obtain the lonicera macranthoides TP. In-vitro experiments show that the purified product has remarkable antioxidant activity, blood sugar lowering activity, acetylcholin esterase inhibition activity and anti-inflammatory activity.

The invention relates to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine effective part composition for treating atopic dermatitis, a preparation as well as a preparation method and application thereof. The composition comprises a flavonoid extract of pimpinella thellungiana, an alkaloid extract of cortex dictamni, a saponin extract of fructus kochiae, a triterpenic acid extract of selfheal, a flavonoid extract of scutellaria baicalensis, a diterpenic acid extract of golden larch bark, a polysaccharide extract of angelica sinensis, a monoterpenoid glycoside extract of radix paeoniae alba, an alkaloid extract of cortex phellodendri and a coumarin extract of fructus cnidii. A sophora flavescens alkaloid extract and a honeysuckle phenylpropanoid extract; the traditional Chinese medicine preparation comprises the composition and auxiliary materials. The invention also discloses application of the composition and the preparation in preparation of medicines for treating atopic dermatitis. The medicinal preparation obtained by the invention can relieve pruritus, relieve inflammatory response, improve dry skin and relieve skin injury, thereby treating atopic dermatitis.

The present invention relates to a mixture, blend or combination, comprising at two, preferably at least three, chosen from the following compounds: (A) a pyrone derivative, (B) a tropolone derivative, (C) a phenylpropanoid, (D) a monoterpene or monoterpenoid, (E) an aromatic acid or salt, (F) a dipeptide, and (G) an alkanediol, and its use as chelating mixture.

The present application relates to the technical field of separation and purification of Schizonepeta tenuifolia, and is a kind of Schizonepeta tenuifolia phenylpropanoid compound, its preparation method and application, Schizonepeta tenuifolia is extracted by heating reflux with ethanol solution and recovered under reduced pressure, and concentrated, the total extract of Schizonepeta tenuifolia is extracted and concentrated with petroleum ether, dichloromethane and ethyl acetate in turn, the extract of ethyl acetate part is separated by gradient elution, the third fraction is separated by gradient elution, the first fraction is separated by gradient elution, the sixth fraction is separated by gradient elution, and phenylpropanoid compound one and phenylpropanoid compound two are obtained at 12.1 minutes and 13.4 minutes, respectively. The present application discloses phenylpropanoid compound one and phenylpropanoid compound two separated from Schizonepeta tenuifolia for the first time, and in vitro anti-inflammatory pharmacodynamic experiments are carried out on the two compounds, which prove that they have a certain inhibitory effect on RAW264.7 cells, and can be applied to the preparation of anti-inflammatory drugs.

The invention discloses a fresh rehmannia root exosome as well as preparation and application thereof. The fresh rehmannia root exosome comprises lipid vesicles filled with rehmannia root metabolites, the rehmannia root metabolites comprise phenylpropanoid and polyketone compounds, and the preparation method comprises the following steps: centrifuging fresh rehmannia root juice and taking supernate; carrying out ultracentrifugation on the supernate, taking precipitate, and resuspending the precipitate by adopting a buffer solution to obtain a first solution; adding the first solution to a concentrated solution of sucrose, and taking liquid at the interface of the concentrated solution of sucrose after ultracentrifugation to obtain a second solution; adding a buffer solution into the second solution, and taking the precipitate after ultracentrifugation to obtain the fresh rehmannia root exosome. Only the exosome extracted from the fresh rehmannia root is used as an active pharmaceutical ingredient and is matched with the modified bone targeting peptide, so that a good osteoporosis treatment effect is achieved, the problems of low active ingredient concentration, large medicinal ingredient extraction loss and poor medicinal effect of single use in the fresh rehmannia root are effectively solved, toxic and side effects of the medicine are avoided, and the curative effect is good. Good patent medicine prospects are realized.

The present invention relates to the enhancement of flumetyl sulforim when it is utilized concomitantly, contemporaneously or in conjunction with Flumetylsulforim-Enhancing compounds, collectively, or typically, Isoprene unit-based compounds or compounds structurally based on 2-methyl-l,3-butadiene units or derived from them, such as, monoterpenoid and derivatives thereof, secondary metabolites originating from isoprene selected from Phenylpropanoids; Monoterpenoids, monoterpenes, flavones, Flavonoids etc., as part of a program to address agricultural and particularly crop plant pathogens, whether in curative, protective, and / or preventative programs. The present invention also relates to methods of increasing the enhancement of flumetylsulforim when it is utilized concomitantly, contemporaneously or in conjunction with Flumetylsulforim- Enhancing compounds collectively, or typically, Isoprene unit-based compounds or compounds structurally based on 2-methyl- 1,3 -butadiene units or derived from them, by adjusting the ratios between the flumetylsulforim and Flumetylsulforim-Enhancing compounds and / or by adjusting the concentration of the compounds in the spray mix from which they are applied.

The present invention relates to the field of the production of phenylpropanoid compounds, especially that of genetically modified strains for the production of phenylpropanoid compounds. In particular, the invention relates to a genetically modified strain of Pseudomonas putida comprising a mutated AroF-I gene encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), and to the use thereof for the synthesis of phenylpropanoid compounds, in particular coumaric acid or frambinone.

The invention discloses a preparation method and application of a polygonatum sibiricum leaf extract antioxidant composition, and belongs to the technical field of natural product extraction and utilization. The method comprises the following steps: by taking waste polygonatum kingianum leaves as a raw material, cleaning, drying at low temperature, breaking walls and crushing, heating, refluxing and extracting by adopting pure water, and performing low-temperature high-speed centrifugal purification and vacuum freeze drying to obtain the high-activity composition. According to the core technology, various active substances such as total sugar, flavone and phenylpropanoid in the polygonatum kingianum leaves are enriched and reserved to the maximum extent through a full-water-based and solvent-free green process, and a synergistic anti-oxidation system is formed. The composition can effectively remove DPPH and ABTS free radicals, inhibit generation of active oxygen in keratinocytes induced by UVB radiation and improve the activity of superoxide dismutase in the cells, and has definite in-vitro anti-oxidation and cell light protection effects. According to the invention, agricultural wastes are recycled, the process is safe, the cost is low, and the obtained composition can be applied to skin antioxidant products such as face cream, essence and the like.

This invention discloses the application of cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins in the preparation of drugs for treating or preventing aging and lowering lipids. In vivo experiments have confirmed that the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention, without reproductive toxicity to a *C. elegans* model, can prolong the average lifespan of nematodes, improve their motility, enhance their stress resistance, reduce endogenous ROS levels and lipofuscin content, and decrease the body fat content of nematodes. Therefore, the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention can be used in the preparation of drugs for treating or preventing aging and lowering lipids.

The invention discloses a quality detection method of a coptis-gardenia medicine pair. According to the quality detection method, analysis conditions such as optimal mobile phase composition, elution procedure, mass spectrum, flow velocity and chromatographic column are screened through a large number of experiments. The chemical components of the coptis chinensis-gardenia medicine pair are analyzed and identified by adopting a UPLC-Q-TOF / MS technology, 99 components are identified, 41 coptis chinensis and 60 gardenia are common, and alkaloids, phenylpropanoids, iridoids, organic acids and flavonoids are taken as main components. On the basis, the UPLC-PDA (Ultra Performance Liquid Chromatography-Personal Digital Assistant) technology is used for carrying out content determination on the main effect components of the coptis-gardenia medicine pair, such as genipin 1-O-gentiobioside, geniposide, coptisine, epiberberine, cotetrandrine, jateorhizine, berberine hydrochloride and palmatine hydrochloride. Systematic methodology investigation and content determination prove that the method is good in precision, stability and repeatability, and reliable technical support is provided for quality control of coptis chinensis-gardenia medicine pairs and basic research of pharmacodynamic substances.

The invention provides a plant lactobacillus, and also discloses a secondary yellow fermented product obtained by fermenting eucommia ulmoides leaves, rehmannia, dried ginger and dendrobium officinale with the plant lactobacillus as well as a preparation method and application of the secondary yellow fermented product. The sec-yellow leavening can directionally activate a phenylpropane biosynthetic pathway and induce the generation of polyphenol characteristic small molecule metabolites, especially the generation of mulberry furan W. The invention also discloses a preparation method of the sec-yellow leavening. And compared with the raw materials, the fermented product has more active small molecule metabolites. Finally, zebra fish experiments after prednisone modeling prove that the sec-yellow yeast can significantly promote osteogenesis. The sec-yellow leavening is recovered to the normal zebrafish gene expression level mainly by regulating the related gene expression level. Therefore, the sec-yellow leavening can be applied to preparation of foods or medicines for improving bone health.

The embodiment of the invention provides a saussurea involucrata cell culture and application thereof. The saussurea involucrata cell culture comprises the following components: a) 7 wt%-20 wt% of a flavonoid compound; b) a phenylpropanoid derivative, the content of which is 2.1 wt% to 14.1 wt%; c) a carboxylic acid and a derivative thereof, wherein the carboxylic acid and the derivative thereof comprise dicaffeoylquinic acid in an amount of 1.6 wt% to 35 wt%; and d) other components.

The present invention relates to a mixture, blend or combination, comprising at two, preferably at least three, chosen from the following compounds: (A) a pyrone derivative, (B) a tropolone derivative, (C) a phenylpropanoid, (D) a monoterpene or monoterpenoid, (E) an aromatic acid or salt, (F) a dipeptide, and (G) an alkanediol, and its use as chelating mixture.

The invention relates to the technical field of component design, in particular to a benzene ring acid group substitution type screening method based on isoamyl cinnamate and application of the benzene ring acid group substitution type screening method based on isoamyl cinnamate. The screening method comprises the steps that a virtual substituent library is established, acid group substituents are introduced to an isoamyl cinnamate benzene ring, and a plurality of substitution combinations are generated; predicting a minimum value pKai of an acid dissociation constant and a polar atom ratio PAR for each structure, and screening less than or equal to 50 candidate structures according to the minimum value pKai and the polar atom ratio PAR; measuring the fluorescence polarization anisotropy r of each candidate structure, and screening the first 50% of samples according to the sequence of r values from small to large; sequentially measuring the quantum dot fluorescence intensity retention rate Fret and the coating UVB retention rate RAUVB of the screened samples; and outputting the acid group substitution type corresponding to the maximum value according to the product sequence of the two. The self anti-ultraviolet attenuation capability and the photosensitive component protection effect are reflected, and the optimal structure is ensured to have self stability and synergistic protection performance in a real compound system.

The present invention relates to a mixture, blend or combination, comprising at least two, preferably at least three, chosen from the following compounds: (A) a pyrone derivative, preferably a 3-hydroxy-4-pyrone, (B) a tropolone derivative, (C) a phenylpropanoid, (D) a mono- or sesquiterpene, or mono- or sesquiterpenoid, (E) an aromatic acid or salt, and (F) an alkanediol, and its use as antimicrobial mixture, blend or combination.

The invention relates to the technical field of medicine detection, in particular to a method for simultaneously detecting 42 chemical components in pediatric nerve-soothing and brain-nourishing granules based on an LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry) technology. The method comprises chromatographic analysis and mass spectrometry. During chromatographic analysis, 3-methoxytyramine is used as an internal standard of alkaloid components, 2-hydroxycinnamic acid is used as an internal standard of organic acid components, albiflorin is used as an internal standard of iridoid glycoside components, tanshinol is used as an internal standard of polygala glycoside and phenylpropanoid components, and myricetin is used as an internal standard of flavonoid components. The method for simultaneously detecting multiple types of components in the pediatric nerve soothing and brain tonifying granules based on the LC-MS / MS technology in combination with a multi-dimensional interior label grouping strategy is applied to content determination of pediatric nerve soothing and brain tonifying granules of different batches, the sensitivity is high, the specificity is high, good precision, repeatability and stability are achieved, and the method is suitable for popularization and application in the field of pediatric nerve soothing and brain tonifying granules. The content difference of the measured chemical components in different batches of the pediatric nerve-calming and brain-nourishing granules can be reflected.

The invention discloses a qi-tonifying, lung-ventilating and phlegm-eliminating traditional Chinese medicine composition for treating acute respiratory distress syndrome. The qi-tonifying, lung-ventilating and phlegm-eliminating traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials: ephedra, astragalus membranaceus, gypsum, roasted rhizoma atractylodis macrocephalae, fructus forsythiae, rhizoma pinellinae praeparata, almond, divaricate saposhnikovia root and liquorice. Chemical components in the traditional Chinese medicine composition of the qi-tonifying, lung-ventilating and phlegm-eliminating decoction are analyzed on the basis of UPLC-Q / TOF-MS, and 233 compounds including 57 flavonoids, 10 phenylpropanoids, 15 unsaturated fatty acid components, 20 triterpenoids, 14 terpene chemical components, 16 alkaloid components, 8 saturated fatty acids, 14 glycosides, 12 tannins, 2 monosaccharide components and 13 steroid components are identified. 13 amino acid components, 4 alkanes, and 35 alkanes in other classes. In addition, an ARDS mouse model induced by LPS and POLY (I: C) mixed nasal dripping is adopted to evaluate the curative effect of the traditional Chinese medicine composition on the ARDS, experimental results show that the qi-tonifying lung-diffusing phlegm-eliminating decoction can remarkably improve pulmonary edema and lung pathological injury of the model mouse, serum biochemical index results show that the qi-tonifying lung-diffusing phlegm-eliminating decoction can reduce inflammatory factors and oxidative stress, and the curative effect of the traditional Chinese medicine composition on the ARDS is improved. The functions of neutrophil are improved, and the traditional Chinese medicine composition has a remarkable effect of treating the acute respiratory distress syndrome.

The invention relates to the technical field of Chinese patent medicine analysis, in particular to a multi-component characterization method of Jichuan decoction and application of the multi-component characterization method. According to the multi-component characterization method provided by the invention, an off-line two-dimensional liquid chromatography tandem quadrupole-electrostatic field orbitrap high-resolution mass spectrometry technology is adopted, and efficient and accurate analysis of various chemical components of Jichuan decoction is realized through specific chromatographic conditions and mass spectrometry conditions. By means of the method, more than two hundred compounds can be identified from the Jichuan decoction, the compounds contain flavonoids, terpenoids, phenylethanoid glycosides, organic acids, coumarins, phenylpropanoids, iridoids, phthalides, steroids and other components, and reference can be provided for research of pharmacodynamic substances of the Jichuan decoction.

The invention belongs to the technical field of biology, and particularly relates to a composition for improving the developmental potential of oocytes, an in-vitro maturation culture solution and an optimized culture method. And a CCR5 / CXCR3 antagonist. The preparation for in-vitro embryo production contains an Ephrin ligand family and a CCR5 / CXCR3 antagonist, the microenvironment for maturation culture of oocytes is synergistically optimized, further, sesquiterpenol compounds and / or phenylpropane compounds can be added on the basis, and therefore the preparation can be used for preparing the embryos in vitro by means of the synergistic effect of the components. The composition can effectively inhibit the formation of abnormal protein aggregates in oocytes, enhance the ability of proteasomes to remove error protein aggregates, significantly reduce the level of reactive oxygen species (ROS) and increase the content of endogenous antioxidant substances such as glutathione (GSH), so as to reduce the protein toxicity stress, oxidative stress and other dimensions, and thus, the composition can be used for preparing an anti-inflammatory drug. The quality and the fertilization rate of oocytes after in-vitro maturation and the development potential of subsequent embryos are remarkably improved, and the application prospect is wide.

The invention discloses lignin-based polyether, a lignin-based composite emulsifier and a preparation method and application of the lignin-based polyether and the lignin-based composite emulsifier. The lignin-based composite emulsifier comprises a modified lignin surfactant A and a modified lignin surfactant B. The modified lignin surfactant A is prepared by degrading lignin to improve activity and then modifying by alkoxy to obtain lignin-based polyether; the modified lignin surfactant B is prepared by degrading lignin to improve the activity and then modifying by long-chain alkyl to obtain hydrophobically modified lignin. The lignin-based composite emulsifier can be applied to the inverse emulsion synthesis process of acrylamide polymers, and can well emulsify and stabilize a polymerization reaction system. The emulsification system expands the raw material source of the surfactant, all the surfactants belong to natural surfactants, are high in safety and biodegradable, and after the acrylamide polymer is injected underground, the phenylpropane structure of the lignin can well emulsify crude oil, so that the emulsification and extraction of the crude oil are facilitated.