The invention discloses an
engineering bacterium for producing
coniferyl alcohol, a construction method and an application of
coniferyl alcohol production. The construction method comprises the following steps: fully chemically compounding TAL, COMT, 4CL and CCR genes; performing
PCR cloning on hpaBC, yahK, metK and pntAB genes; fully chemical compounding pBPE001, pBPE002 and pBPE003; linking the genes TAL, hpaBC and COMT with pBPE001, thereby acquiring pTHM; linking the genes 4CL, CCR and yahK with pBPE002, thereby acquiring pLRH; linking the genes pntAB and metK with pBPE003, thereby acquiring pNAM; integrating the T7
RNA polymerase gene into SyBE-002447 base strain, thereby acquiring a SyBE-002447(DE3) strain; converting pTHM, pLRH and pNAM into SyBE-002447(DE3), thereby acquiring the
engineering bacterium SyBE-CNF; taking glucose as a
carbon source and fermenting, thereby producing
coniferyl alcohol. According to the invention, the
engineering escherichia coli are used for producing coniferyl
alcohol, so that the source problem of the coniferyl
alcohol can be solved and the cost can be lowered.