73 results about "Monolignol" patented technology

This invention relates to a method for extracting valorized compounds from lignin by contacting lignins with an ionic liquid and/or a deep eutectic solvent and adding a catalyst and/or a biocatalyst to assist in breaking down the source material. Converting lignin into high value chemicals adds revenues for a bio-refinery and helps to improve the economic viability of biofuel production.

The invention relates to a technique for extracting lignin, in particular to a technique of utilizing organic acid to extract the lignin from biomass raw materials by a catalytic action of hydrogen peroxide. According to the method, the lignin is obtained by extracting the biomass raw materials boiled by organic acid formed by methanoic acid and acetic acid by the catalytic action of hydrogen peroxide; a whole technique route is simple; the energy consumption is lower; and an extraction ratio of the lignin reaches 80-90%.

This invention relates to a process for production of ethanol from lignocellulosic material. Lignocellulosic material treated with a dicarboxylic acid, preferably with oxalic acid, separating a hemicellulosic fraction to ferment pentose sugar. The lignin is dissolved in alkali, preferably with NaOH, separating a cellulose fraction for further enzymatic treatment with one or more than one cellulytic enzymes capable of hydrolyzing cellulose. The enzyme hydrolyasate is further subjected to fermentation in the presence of ethanol-producing yeast, preferably Sacchoromyces cerevisiae. The fermented broth is further subjected to distillation followed by dehydration to yield ethanol.

Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant roculture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.

The invention provides a new method for converting a lignin model compound dimer and a lignin raw material to a corresponding aromatic compound through a hydrogen transfer reaction by taking ReO<x>/AC as a catalyst. According to the method, one or two micromolecular alcohol is taken as a reaction regent, ReO<x>/AC is taken as a catalyst, and the lignin model compound and the lignin raw material are catalyzed and converted to a micromolecular aromatic chemical in a mild condition. Compared with a traditional lignin depolymerization method, the method has distinct features: no addition of hydrogen and oxygen sources, mild reaction conditions, high selectivity of monophenol products, fast reaction speed, recyclable catalyst, and simple operation. The aromatic chemicals can be prepared from renewable lignin sources, a mild depolymerization strategy without consuming hydrogen sources is developed, and a new approach is developed for producing an aromatic compound in a non-petroleum route.

Described is an isolated lignified plant cell wall including lignin, wherein the lignin includes a ferulate residue incorporated therein, such as from coniferyl ferulate and/or sinapyl ferulate. Also described is a method to make the isolated lignified plant cell wall, and the lignin produced by the method.

The invention relates to a method for preparing syringaldehyde by oxidative degradation of lignin, which comprises the following steps of: 1) dissolving an oxidative degradation product solid of the lignin by using an organic solvent, adding ammonia water to obtain a syringaldehyde and ammonia water complex precipitate, performing centrifugal separation, and acidifying the complex precipitate to obtain syringaldehyde; and 2) evaporating to dryness under reduced pressure and centrifuging to obtain ammonia water mixed solution, performing bromination on the obtained solid substance and elemental bromine or bromine hydride solution, and performing methoxylation reaction on the bromination product and sodium methoxide under the catalysis of cuprous salt to obtain the syringaldehyde. By the steps, the oxidative degradation product of the lignin is all converted into the same product, namely the syringaldehyde, the yield of the syringaldehyde is 50-60 percent, the utilization rate of lignocellulosic materials is improved, the cost of cellulose alcohol and papermaking industry is reduced, and the method has important meaning for realizing high value-added industrial development of the lignin and industrialization of cellulose ethanol and promoting agricultural sustainable development.

The invention relates to a method for selectively producing lignin-degrading enzymes by using phanerochaete chrysosporium. The invention provides a method for selectively producing lignin-degrading enzymes by using phanerochaete chrysosporium, which comprises the following steps: fermenting phanerochaete chrysosporium in a culture medium containing an immobilized culture carrier in a non-immersed state, and collecting a fermentation product, namely, a lignin-degrading enzyme. Experiments show that when the method is adopted for carrying out fermentation on a lignin-degrading enzyme, the lignin-degrading enzyme can be selectively obtained under air conditions without feeding pure oxygen or air containing high-concentration oxygen in the process of culturing but just by controlling the concentration of a carbon and nitrogen source in the culture medium and adding a carrier in a non-immersed state. The method disclosed by the invention is simple in condition control, and the obtained lignin-degrading enzyme is strong in selectivity.

The invention relates to a method for oxidative degradation of lignin into aromatic monomers through molybdenum catalysis. According to the method, the oxidative degradation of the lignin is catalyzedby taking molecular oxygen as an oxygen supply and taking a non-noble metal molybdenum compound as a catalyst. According to the method, the catalytic oxidation system is simple in composition and isenvironmentally friendly, the operating conditions are moderate, and the yield of the aromatic monomers (mainly aromatic aldehyde, aromatic ketone and aromatic acid ester) in a degradation product ishigh; and possibility is provided for the high-value utilization of native lignin and even lignocellulose.

A process for the isolation of lignin from black liquor and modification of lignin for use in many plastic applications is disclosed. The isolation of lignin consists of removing all of the non-lignin components from black liquor solution. The non-lignin components including but not limited to organic acids, sugars, and inorganic materials can be removed using either solvent extraction or ion-exchange resin or a combination of both methods. The isolated lignin is water soluble. The non-lignin components can be further isolated and sold or reused in the pulping or lignin isolation processes. The isolated lignin can be further modified in order to meet the needs of the desired plastic application.

The invention provides a method for preparing phenolic chemicals by catalyzing degradation of lignin with carbon-based solid acid. The method comprises the following steps: by taking lignin as a raw material, a carbon-based solid acid as a catalyst and a water phase as a reaction solvent, putting lignin, carbon-based solid acid and water into a high-pressure reaction kettle; and carrying out warming reaction under the protection of an inert gas, and then extracting by using ethyl acetate, and performing vacuum evaporation so as to obtain the phenolic chemicals, wherein used carbon-based solid acid is prepared by using the lignin as the raw material in an inorganic acid environment; and the lignin raw material for carbon-based solid acid is alkali lignin, organic lignin, zymolytic lignin residue or hydrolytic lignin residue. The phenol yield can be up to 53%, the relative selectivity of guaiacol aromatic products can be up to 70%, the technique disclosed by the invention is simple and environmental friendly, available in raw materials and low in demands on equipment, and does not need to use a concentrated acid with strong corrosivity, and compared with the prior art, the method has outstanding advantages.

A process for producing an organic aliphatic product (such as butanol) from lignocellulosic biomass is provided, comprising: (a) fractionating lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; (b) washing the cellulose-rich solids and separating the cellulose-rich solids from the liquor; (c) enzymatically hydrolyzing the cellulose-rich solids to generate a hydrolysate comprising glucose; (d) detoxifying the hydrolysate by neutralizing the hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate; (e) fermenting the purified hydrolysate using a suitable microorganism to produce a dilute organic aliphatic product, wherein the microorganism is recycled with a membrane; (f) extracting the dilute organic aliphatic product into a water-immiscible extractant, to generate an intermediate material; and (g) distilling the intermediate material to generate a concentrated organic aliphatic product.

The invention discloses a method for preparing a monophenol compound through catalyzing degradation of lignin by using an ammino-complex. The method comprises the steps: adding the lignin, a metal salt, ammonia water, a peroxidant, an alkali and deionized water into a high-pressure reaction vessel, and carrying out stirring to form a uniform solution; carrying out a reaction for 100 to 240 minutes at the temperature of 120 DEG C to 180 DEG C, and carrying out cooling; and adjusting the pH value of a reaction solution to 2 to 3 with an acid, then, carrying out a reaction for 10 to 20 minutes at the temperature of 100 DEG C to 120 DEG C, carrying out cooling, then, carrying out filtration to collect filtrate, carrying out extraction on the filtrate with dichloromethane, separating an organic phase, and carrying out drying with anhydrous calcium chloride, thereby obtaining a monophenol compound mixed solution. According to the method, a stable ammino-complex solution is formed by using the metal salt and the ammonia water under alkaline conditions, so that the number of sites of contact between a catalyst and the lignin can be effectively increased compared with that in the conventional lignin oxidative degradation method; the degradation of the lignin can be efficiently catalyzed in an aqueous solution under moderate reaction conditions; and the method has the characteristics of high efficiency and low cost and is applicable to large-scale industrial production.

The following invention generally relates to a melt flowable biocarbon polymeric material derived from a cellulosic ethanol refining co-product, monolignol biopolymer, and a heat processed or thermally modified biomass flour, both of which are reacted together to create a melt flowable biopolymer which has melt flowable properties, process-ability and rheology similar to that of standard petrochemical based thermoplastics.

Belonging to the technical field of biomass degradation, the invention relates to a method for degrading oxidation lignin into a small molecular aromatic compound, and in particular relates to a method of breaking C-C bond by nontransition metal catalysis to degrade oxidation lignin into a small molecular aromatic compound. The method includes: firstly oxidizing oxidation lignin in an organic solvent with an oxidizing agent, then subjecting the obtained intermediate product to alcoholysis or hydrolysis under the action of a catalyst so as to obtain a small molecular aromatic compound, like methoxy substituted benzoic acid, methoxy substituted phenol, methoxy substituted benzoate and chain alcohol, etc. The method provided by the invention has the advantages of simple steps, mild reaction conditions, high conversion rate (up to 100%), no need for transition metals and the like, has effect on multiple connection modes of oxidation lignin, can reach the purpose of high selectivity and efficient degradation of lignin, and is conducive to large-scale industrial application.

Novel genes that code for a family of feruloyl esterases that break down ferulic acid crosslinks between polysaccharide chains and between polysaccharides and lignins in plant cell walls are described herein as well as a method of rapid gene discovery.

The invention relates to a method for catalyzing oxicracking of aryl ethers of a lignin model. The method is characterized by comprising the step of catalyzing the oxicracking of the aryl ethers of the lignin model by taking two components, i.e., a simple inorganic oxovanadium compound and nitrate as a composite catalyst system and taking molecular oxygen as an oxygen supply. According to the method, the catalyst is simple, cheap and readily available, the reaction conditions are mild, the efficiency of oxicracking is high, and the aryl ethers in lignin can be efficiently cracked by a catalyzed oxidation method.

The invention relates to selective depolymerization of lignin hydrogenation, in particular to application of a monatomic catalyst in the catalytic hydrogenation of lignin to an aromatic compound. According to the method, a plurality of beta-O-4 model molecules and organic lignin, lignosulfonate and alkali lignin are used as raw materials, and the aromatic compound is obtained by highly selective cracking of an aryl ether bond in the hydrogen atmosphere of 150-240 DEG C and 0.7-3.0 MPa. The conversion rate of the beta-O-4 model molecules reaches up to 99%, the yield of guaiacol reaches up to 93%, and the yield of styrene reaches up to 65%. When different lignins are used as the raw materials for conversion, the yield of aromatic bio-oil is 38-70%. Renewable natural biomass is as the raw material, and the raw material is cheap and has a wide source; there is no need to use inorganic acid and alkali in the reaction process, and a lot of alkali lye by traditional lignin catalysis is avoided; and the reaction condition is mild, activity and selectivity are high, and the reaction process is environmentally-friendly.

The invention provides a method for separating and extracting cellulose nanocrystals and lignin from poplar powder, and belongs to the field of natural polymer material separation and extraction. According to the method, 1-6 parts by mass of the poplar powder is treated by a mixed acid system which is prepared from the components in parts by mass: 60-90 parts of organic acid, 40 parts of distilledwater and 5-20 parts of concentrated sulfuric acid with mass fraction being 98%, the reaction temperature is 70 DEG C-80 DEG C and the reaction time is 6h-8h, the organic acid is one of oxalic acid,formic acid and acetic acid, after the reaction is completed, the obtained lignin-containing cellulose nanocrystals are subjected to alkali treatment, and the cellulose nanocrystals and the lignin areseparated. The method omits the step of preparing the cellulose nanocrystals to perform pretreatment and delignification on fiber raw materials, and simultaneously obtains two products of the cellulose nanocrystals and the lignin. The process is simple and the cost is reduced.

The invention belongs to the technical field of plant gene engineering, and particularly relates to GhLac1 gene of broad-spectrum resistance of mediated cotton. According to the invention, the nucleotide sequence of the GhLac1 gene is as shown by SEQ ID NO:1, the sequence of amino acid of coded protein thereof is as shown by SEQ ID NO:2, and an open reading frame thereof is the sequence corresponding to 20-1720th basic group shown by the SEQ ID NO:1. The invention also discloses an application of the gene in cotton heredity and transformation. Through excessive expression of the gene, the synthesis of lignose in the plant can be promoted, so as to accelerate lignification process, and meanwhile, to accelerate reconstruction of cell walls of damaged cells. The cloned gene provided by the invention can be applied to the select breeding of broad-spectrum disease resistance or insect-resistant novel varieties through heredity and transformation.