5results about How to "Mild method conditions" patented technology

This invention discloses a method for preparing and applying a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure. Based on an epitaxial layer-by-layer growth strategy, this invention constructs a multi-layered core-shell structure to obtain a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure, ZIF-8@coumarin@ZIF-8@rhodamine B, exhibiting an "inner layer reference - outer layer response" relationship. The nanoprobe prepared by this invention not only suppresses signal crosstalk caused by fluorescence resonance energy transfer but also achieves spatial selectivity of the inner filtration effect. Based on the characteristic absorption of malachite green in the 600-620 nm range, it can selectively quench the fluorescence of the outer rhodamine layer while maintaining a stable signal from the inner coumarin layer, thus achieving ratiometric fluorescence detection. This detection method has advantages such as strong anti-interference ability, high signal stability, and high sensitivity, and can be used for the rapid detection of malachite green in aquatic products.

The present application relates to a kind of expression osmanthus source carotene cleavage dioxygenase (OfCCD1) recombinant yeast engineering bacteria and its application in tobacco flavoring, the amino acid sequence of OfCCD1 is as shown in SEQ ID NO:1.The present application is based on pPICZ alpha A vector constructs recombinant overexpression dioxygenase CCD Pichia engineering bacteria, and the application of the engineering bacteria in improving the content of tobacco beta-ionone and improving the sensory quality of tobacco.Compared with multiple plant source dioxygenase engineering bacteria constructed in the same group, the osmanthus source OfCCD1 engineering bacteria constructed is applied to carotene precursor in tobacco, and aromatic substance is cracked to generate fragrance marker beta-ionone, relative to untreated group, it is increased by 57.1%, significantly improve the quality of aroma, satisfaction, salivation and other indicators, and the application effect is best.The method for improving the quality of tobacco of the present application is mild, green and efficient, and has good application prospect.

This invention discloses Aspergillus terreus Aspergillus terreus Applications for reducing nicotine content. Specifically, these applications include Aspergillus terreus. Aspergillus terreus This invention relates to the method for reducing nicotine content in tobacco raw materials, tobacco waste, or tobacco processing byproducts. The method is characterized by high degradation efficiency, mild conditions, and environmental friendliness, providing a new and effective tool for biologically reducing nicotine content in tobacco leaves. It shows promising application prospects in improving tobacco quality and reducing harmful substances and tar content.

The invention discloses a 2-x-terephthalic acid copper-based MOFs (Metal-Organic Frameworks) micro-nano sheet and a preparation method and application thereof, and the method comprises the following steps: dropwise adding a copper chloride dihydrate solution into a pre-deprotonated 2-x-terephthalic acid solution, stirring to react, and centrifugally washing and drying the product to obtain the 2-x-terephthalic acid copper-based MOFs micro-nano sheet, x is one of methyl, amino or hydroxyl. The 2-x-terephthalic acid copper-based MOFs micro-nano sheet can be used for electrocatalytic reduction of carbon dioxide. The 2-x-terephthalic acid copper-based MOFs micro-nano sheet prepared by the invention has a unique folded sheet structure, the specific surface area and the exposure degree of active sites of the material are remarkably increased, and mass transfer and charge transfer paths are shortened, so that the kinetics and energy efficiency of a catalytic reaction are integrally optimized.

The present application relates to the field of environmental protection, and discloses a method for degrading formamide organic wastewater and recovering key metals from retired lithium ion batteries. The method comprises the following steps: S1, obtaining a positive electrode material of a retired lithium ion battery, mixing the positive electrode material with formamide organic wastewater, and performing a hydrothermal reaction to obtain a leaching solution; S2, adjusting the pH of the leaching solution to 6-14, and performing a transition metal precipitation reaction to obtain a transition metal hydroxide precursor and a lithium-rich filtrate; and S3, adding a sodium carbonate solution to the lithium-rich filtrate, performing a precipitation reaction, and collecting lithium carbonate. The formamide organic wastewater acts as an endogenous reducing agent, and under hydrothermal conditions, it achieves deep degradation of itself while achieving efficient leaching of key metals in the retired lithium ion battery, thereby achieving waste treatment with waste. The degradation efficiency of formamide can reach more than 90%, overcoming the defects of high energy consumption, large reagent consumption, high cost, low degradation efficiency and large environmental treatment pressure of existing formamide wastewater treatment methods.