14results about How to "Mild method conditions" patented technology

The invention relates to the technical field of perovskite quantum dots, in particular to a method for preparing perovskite quantum dots through a normal-temperature solution reaction method. The preparation method comprises the following steps: dissolving a first ligand and a cation precursor in a first solvent, and then diluting with a second solvent to prepare a cation-ligand solution; dissolving a second ligand and lead salt in a first solvent, and then diluting by using a second solvent to prepare a lead salt-ligand solution; and mixing the lead salt-ligand solution with a second solvent, adding the cation-ligand solution under the condition of violent stirring to obtain a reaction system, and then reacting under the condition of normal temperature to obtain the perovskite quantum dots. The perovskite quantum dots can be prepared through a solution reaction method under a normal temperature condition, harsh conditions of a hot injection method and use of a large amount of an anti-solvent of a ligand-assisted precipitation method are avoided, rapid cooling from a high temperature is not needed, the quantum dots can be synthesized without using the anti-solvent, and the preparation method is simple and convenient. The defect that large-scale and large-batch production of the perovskite quantum dots is difficult to realize by the existing synthesis method can be overcome.

The invention belongs to the field of medicinal chemistry, and particularly relates to an 8-position hydroxyurolithin A derivative as well as a preparation method and application thereof. According to the method, 2-bromo-5-(benzyl methoxy)-benzoic acid is prepared from 2-bromo-5-hydroxybenzoic acid through hydroxyl protection, then cyclization, etherification and deprotection reaction are carried out, the reaction conditions are milder, the reaction time is shorter, the yield is higher, groups sensitive to acid and alkali and various substrates can well tolerate the reaction conditions, and the method is suitable for industrial production. The 8-site hydroxyurolithin A derivative is prepared, the obtained derivative is targeted to PDE2 small molecules, and the 8-site hydroxyurolithin A derivative has huge potential for treating neurodegenerative diseases such as Alzheimer's disease.

The invention relates to an efficient preparation method and application of optically pure beta-cyanone. The invention belongs to the technical field of fine chemical synthesis. The invention aims to solve the technical problems of low yield, long reaction time, harsh reaction conditions and unsuitability for industrial production of the existing method for preparing optically pure beta-cyanone through asymmetric conjugate cyanidation addition reaction. According to the method, an alpha, beta-unsaturated carbonyl compound is taken as a substrate, acetone cyanohydrin is taken as a cyanogen source, and under the action of a cinchona alkali derivative, alkali and an additive, the optically pure beta-cyanoketone is prepared by heating in a nitrogen atmosphere in one step without tedious operation. By screening cinchona alkaloid derivatives, alkali, additives, reaction temperature and reaction solvents and coordinating and controlling the optimal proportion of all the substances, side reactions are inhibited to the maximum extent, beta-cyanoketone is obtained with high stereoselectivity and yield, and possibility is provided for industrial production of optically pure beta-cyanoketone. The method is mild in condition, simple in operation process, short in reaction time and environmentally friendly, acetone cyanohydrin serves as a cyanogen source, substances such as highly toxic sodium cyanide, TMSCN low in atom utilization rate and high in price are replaced, the application range of a substrate is good, and the method can be widely applied to the fields of biochemistry and medicinal chemistry.

The invention discloses a preparation method and application of a post-modified metal organic framework material for catalytically oxidizing benzyl alcohol into benzaldehyde, and belongs to the technical field of photocatalytic materials. Zr-based MOF-808 is used as a parent framework, organic dye eosin Y (eosin Y, EY) is used as a functional unit, and the parent framework and the functional unit are coordinated and anchored to obtain the post-modified metal organic framework material EY-MOF-808. The post-modified metal organic framework material EY (at) MOF-808 has abundant unsaturated coordination sites, provides a way for distinguishing the combining capacity of objects with different groups and improving reactivity and selectivity, and effectively avoids excessive oxidation of benzaldehyde. The EY-coated MOF-808 is suitable for catalyzing benzyl alcohol with a plurality of substituent groups, and corresponding benzaldehyde is obtained at the selectivity of more than 99%. In addition, the EY-coated MOF-808 can be recycled for four times, the selectivity is not reduced, and toxic dyes are prevented from being released into the environment.

This invention discloses a method for synthesizing carboxymethylcysteine, belonging to the field of organic synthesis technology. The method includes steps such as raw material dissolution, condensation reaction, neutralization crystallization, and centrifugal drying. The method of this invention directly neutralizes the reaction solution to the target pH with concentrated hydrochloric acid, stirs to precipitate crystals, and centrifuges and filters to directly obtain a qualified product, eliminating the need for multiple crystallization purifications. This reduces processes and equipment, increases yield, reduces material consumption, and significantly lowers process costs. The purity of the obtained product reaches over 99.90%, the residue on ignition content is as low as 0.05%, and the contents of cysteine, cystine, lactam, dicarboxymethylcysteine, and ammonium salts are extremely low; moreover, the yield reaches over 95%. The method of this invention operates under mild conditions, with no harmful gas emissions, and is equipment-friendly.

The invention relates to the technical field of biosynthesis, and provides a synthesis method of L-2-amino adipic acid-delta-semialdehyde, which comprises the following steps: taking L-lysine and alpha-ketoglutaric acid as substrates, and carrying out transaminase catalytic reaction to obtain L-2-amino adipic acid-delta-semialdehyde; the transaminase is one of L-lysine 6-amino transferase and a mutant of the L-lysine 6-amino transferase; the L-lysine 6-aminotransferase is derived from Stenotrophomonas koreensis, and the amino acid sequence of the L-lysine 6-aminotransferase is as shown in SEQ ID No. 2; the N end and / or C end of the protein limited by SEQ ID No.2 is connected with a label to obtain the fusion protein. According to the technical scheme, the problem of low conversion efficiency of L-2-amino adipic acid-delta-semialdehyde in related technologies is solved.

This invention discloses a method for preparing and applying a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure. Based on an epitaxial layer-by-layer growth strategy, this invention constructs a multi-layered core-shell structure to obtain a dual-fluorescent ZIF-8 nanoprobe with a spatially compartmentalized structure, ZIF-8@coumarin@ZIF-8@rhodamine B, exhibiting an "inner layer reference - outer layer response" relationship. The nanoprobe prepared by this invention not only suppresses signal crosstalk caused by fluorescence resonance energy transfer but also achieves spatial selectivity of the inner filtration effect. Based on the characteristic absorption of malachite green in the 600-620 nm range, it can selectively quench the fluorescence of the outer rhodamine layer while maintaining a stable signal from the inner coumarin layer, thus achieving ratiometric fluorescence detection. This detection method has advantages such as strong anti-interference ability, high signal stability, and high sensitivity, and can be used for the rapid detection of malachite green in aquatic products.

The present application relates to a kind of expression osmanthus source carotene cleavage dioxygenase (OfCCD1) recombinant yeast engineering bacteria and its application in tobacco flavoring, the amino acid sequence of OfCCD1 is as shown in SEQ ID NO:1.The present application is based on pPICZ alpha A vector constructs recombinant overexpression dioxygenase CCD Pichia engineering bacteria, and the application of the engineering bacteria in improving the content of tobacco beta-ionone and improving the sensory quality of tobacco.Compared with multiple plant source dioxygenase engineering bacteria constructed in the same group, the osmanthus source OfCCD1 engineering bacteria constructed is applied to carotene precursor in tobacco, and aromatic substance is cracked to generate fragrance marker beta-ionone, relative to untreated group, it is increased by 57.1%, significantly improve the quality of aroma, satisfaction, salivation and other indicators, and the application effect is best.The method for improving the quality of tobacco of the present application is mild, green and efficient, and has good application prospect.

This invention discloses Aspergillus terreus Aspergillus terreus Applications for reducing nicotine content. Specifically, these applications include Aspergillus terreus. Aspergillus terreus This invention relates to the method for reducing nicotine content in tobacco raw materials, tobacco waste, or tobacco processing byproducts. The method is characterized by high degradation efficiency, mild conditions, and environmental friendliness, providing a new and effective tool for biologically reducing nicotine content in tobacco leaves. It shows promising application prospects in improving tobacco quality and reducing harmful substances and tar content.

The invention discloses a 2-x-terephthalic acid copper-based MOFs (Metal-Organic Frameworks) micro-nano sheet and a preparation method and application thereof, and the method comprises the following steps: dropwise adding a copper chloride dihydrate solution into a pre-deprotonated 2-x-terephthalic acid solution, stirring to react, and centrifugally washing and drying the product to obtain the 2-x-terephthalic acid copper-based MOFs micro-nano sheet, x is one of methyl, amino or hydroxyl. The 2-x-terephthalic acid copper-based MOFs micro-nano sheet can be used for electrocatalytic reduction of carbon dioxide. The 2-x-terephthalic acid copper-based MOFs micro-nano sheet prepared by the invention has a unique folded sheet structure, the specific surface area and the exposure degree of active sites of the material are remarkably increased, and mass transfer and charge transfer paths are shortened, so that the kinetics and energy efficiency of a catalytic reaction are integrally optimized.

The present invention provides a method for synthesizing an On-DNA aryl alkyne lead compound, and belongs to the field of gene-encoded compound library construction. The method uses an oligonucleic acid-terminal alkyne compound and an arylboronic acid as raw materials, and reacts in the presence of a copper catalyst and a base to obtain an On-DNA aryl alkyne lead compound. The method for synthesizing an On-DNA aryl alkyne lead compound of the present invention has low cost, little damage to DNA, good universality, simple operation, mild conditions, and can obtain On-DNA aryl alkyne compounds in high yield, and has broad application prospects in constructing a gene-encoded compound library.

The invention discloses a method for synthesizing menthol amine compounds and their application in cigarettes. In an air environment, menthol and bromoacetyl bromide are placed in a reaction container, a first additive is added, a first step reaction is carried out in a first solvent, and then an amine derivative and a second additive are added, and a reaction is carried out in a second reaction solvent to prepare menthol amine compounds, wherein the reaction formula is: The menthol amine compound prepared by the invention is a common biologically active molecular structure and has a wide range of biological properties, including antibacterial, anti-inflammatory and antiviral activities. In particular, the menthol amine compound prepared by the invention has a more penetration-promoting effect, promotes the transdermal penetration of drugs, and is non-irritating and non-toxic to the skin.

The present application relates to the field of environmental protection, and discloses a method for degrading formamide organic wastewater and recovering key metals from retired lithium ion batteries. The method comprises the following steps: S1, obtaining a positive electrode material of a retired lithium ion battery, mixing the positive electrode material with formamide organic wastewater, and performing a hydrothermal reaction to obtain a leaching solution; S2, adjusting the pH of the leaching solution to 6-14, and performing a transition metal precipitation reaction to obtain a transition metal hydroxide precursor and a lithium-rich filtrate; and S3, adding a sodium carbonate solution to the lithium-rich filtrate, performing a precipitation reaction, and collecting lithium carbonate. The formamide organic wastewater acts as an endogenous reducing agent, and under hydrothermal conditions, it achieves deep degradation of itself while achieving efficient leaching of key metals in the retired lithium ion battery, thereby achieving waste treatment with waste. The degradation efficiency of formamide can reach more than 90%, overcoming the defects of high energy consumption, large reagent consumption, high cost, low degradation efficiency and large environmental treatment pressure of existing formamide wastewater treatment methods.

The invention relates to the technical field of arsenic-containing wastewater treatment, in particular to a method for reducing arsenic-containing wastewater by ultraviolet light and extracting and separating elemental arsenic. According to the method for reducing the arsenic-containing wastewater through the ultraviolet light and extracting and separating the elemental arsenic, after the arsenic-containing wastewater and a reducing agent are mixed, photo-reduction is conducted under the normal-temperature and normal-pressure conditions, and the elemental arsenic is obtained; adding a surfactant into the dispersion phase containing the elementary substance arsenic, and extracting and separating the product elementary substance arsenic through cloud point extraction; the reducing agent is sulfite and halide. The method disclosed by the invention is mild in condition, high in arsenic reduction rate and wide in pH range of applicable wastewater, and the prepared product is high in purity.