467 results about "CHITOSAN OLIGOSACCHARIDE" patented technology

The invention relates to the technical field of cosmetics, and provides a composition containing a dendrobium officinale enzymolysis extract, which is prepared from the following components in percentage by mass: 18 to 22 percent of dendrobium officinale enzymolysis extract, 9 to 11 percent of chitosan oligosaccharide, 6.5 to 7.5 percent of bionic moisturizing enhancer, 0.1 to 0.2 percent of sodium alginate, 0.05 to 0.15 percent of citric acid / sodium citrate buffer pair and 60.4 to 65.4 percent of solvent matrix. By combining low-temperature enzymolysis with a biological purification process, efficient extraction of dendrobium candidum polysaccharides and phenolic substances is realized, dendrophenol oxidation inactivation caused by high temperature can be effectively avoided, hydrochloric acid residues in chitosan oligosaccharide preparation are effectively removed, formula irritation is remarkably reduced, and the dendrobium candidum extract is more suitable for sensitive skin care; the components in the composition have a synergistic effect, the dendrobium officinale enzymolysis extract and the enzymolysis chitosan oligosaccharide form a composite moisturizing network, the moisturizing rate is remarkably increased, the oxidation resistance is remarkably improved, and the composition can be applied to products such as essence, masks and freeze-dried powder.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention relates to the technical field of textile material and fiber processing, in particular to a high-elasticity moisture-absorption breathable acetate fabric and a preparation method thereof. The invention discloses a high-elasticity moisture-absorption breathable acetate fabric and a preparation method thereof.The acetate fabric takes a bio-based degradable thermoplastic polyester elastomer as an island and acetate as a sea, a homothermal double-hole structure is embedded in the acetate fabric, and the surface of the acetate fabric is covered with a polyglutamic acid and chitosan oligosaccharide self-repairing hydrophilic layer; the preparation method comprises the following steps: pre-burying alkali-resistant microspheres in a marine facies through solid island-solution sea double-path spinning of a bio-based degradable thermoplastic polyester elastomer and acetate, synchronously forming groove-closed pore double channels through simultaneous heating one-step alkali etching-baking, and finally forming a dynamic reversible hydrogen bond network through polyglutamic acid and chitosan oligosaccharide, so as to obtain the marine-based degradable alkali-resistant hydrogel. A bio-based finishing layer with a self-repairing function is obtained, and the finally obtained fabric has the characteristics of high elasticity, breathability, lasting moisture absorption, greenness and degradability.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention discloses a bran fermented biological feed and a preparation method thereof, and belongs to the technical field of feeds. The bran fermented biological feed is prepared by uniformly mixing and fermenting the following raw materials: wheat bran, corn flour, soybean meal, calcium hydrogen phosphate, sodium chloride, an enzyme preparation, a water retention setting agent, a mildew preventive and water, wherein the enzyme preparation is prepared from lactobacillus plantarum, bacillus subtilis and saccharomycetes; the mildew preventive is prepared by the following steps: reacting chitosan oligosaccharide with propiolic acid to obtain modified chitosan oligosaccharide, reacting L-cystine with ethyl azidoacetate to obtain an azide compound, and finally reacting the modified chitosan oligosaccharide with the azide compound. The prepared bran fermented biological feed has an excellent mildew-proof effect and a low pulverization rate, and storage and transportation of the feed are facilitated.

The invention relates to the technical field of feed processing, and discloses a lactating sow feed containing fermentation raw materials and a preparation method of the lactating sow feed. A three-layer microcapsule coating auxiliary material is prepared by taking a vitamin premix, an amino acid compound, a compound enzyme preparation and a probiotic agent as core materials through three-layer coating; the basic raw materials comprise corn, wheat, bran, a fermentation raw material, a mineral premix and grease, auxiliary material coating and basic raw material treatment are carried out in parallel in double lines during preparation, and finally, mixing granulation is carried out; the grafted copolymer formed by the amidation reaction of the sodium carboxymethyl cellulose and the chitosan oligosaccharide can cooperate with the sodium alginate to prevent the damage under acidic conditions, and the survival rate of viable bacteria is kept at 85-87% after treatment is carried out for 2 hours under the acidic condition with the pH value of 2.0-3.0; compared with a traditional fermentation raw material coating scheme, the dosage of coating raw materials is reduced by 70% or above; meanwhile, the vitamin premix and the amino acid compound in the auxiliary material can reduce the diarrhea rate by 6% and increase the lactation yield of the sow by 7-18%.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention discloses a macrophage-polymer lipid nanoparticle composite drug delivery system, a preparation method thereof and application of the macrophage-polymer lipid nanoparticle composite drug delivery system in cryptococcus infection resistance. According to the drug delivery system, alpha-linolenic acid (ALA), high molecular weight chitosan oligosaccharide (HCOS) and amphotericin B (AmB) are used as raw materials to construct nanoparticles AmB-PLHNs, the nanoparticles are loaded in macrophages through the phagocytosis of the macrophages, and the AmB-PLHNs (at) M1 is constructed. AmB is a common medicine for clinically treating Cn infection, but has the problems of toxic and side effects on kidney and incapability of being transferred into the center. The AmB-PLHNs (at) M1 carries AmB-PLHNs to be transferred into the brain according to the blood-brain barrier penetrating characteristic of M1 type macrophages, and the brain entering efficiency of the medicine is improved. By combining the phagocytosis of macrophages on cryptococcus, the inflammation immune regulation function of the macrophages and the bacteriostatic action of the drug-loaded nanoparticles, the drug-loaded nano-particles are used for treating Cn central infection, and the toxic and side effects of AmB are reduced.

The invention relates to a composite blocking remover for an oil field and a preparation method thereof, and belongs to the technical field of oil exploitation. The composite blocking remover is prepared from the following components: 18 to 22 weight percent of composite acid, 2.7 to 3.5 weight percent of a modified chitosan oligosaccharide synergist, 0.35 to 0.52 weight percent of a surfactant, 0.1 to 0.12 weight percent of a corrosion inhibitor and the balance of water, according to the modified chitosan oligosaccharide synergist, chitosan oligosaccharide is used as a rigid base chain, a supporting effect is provided, double chains are prevented from being intertwined in a water-based environment, a polythioamide chain containing an alkyl quaternary ammonium structure is used as a functional auxiliary chain, redeposition of dissolved matter is relieved through gel breaking-chelation integration, meanwhile, a chain structure forms an adhesive tape effect, and the synergistic effect is achieved. And the blockage removing driving force is increased, so that efficient blockage removing is realized.

The invention discloses a catechin-chitosan oligosaccharide-nano selenium composite material as well as a preparation method and application thereof, and belongs to the technical field of biomass nano materials. The preparation method of the catechin-chitosan oligosaccharide-nano selenium composite material comprises the following steps: firstly, mixing an aqueous solution of sodium selenite and an aqueous solution of epigallocatechin gallate according to the molar ratio of 1: (10-25), and stirring; the catechin-chitosan oligosaccharide-nano-selenium composite material is prepared by adding the catechin into sodium selenite, then adding chitosan oligosaccharide and water, and reacting to obtain the catechin-chitosan oligosaccharide-nano-selenium composite material, the final concentration of the sodium selenite in the reaction system is 1mM, and the final concentration of the chitosan oligosaccharide in percentage by mass is 1-2%. The composite material provided by the invention has relatively excellent antibacterial activity, is easy to clean, natural and environment-friendly, the preservation effect of the composite material applied to the berry fruits is superior to the effect of chitosan under the same concentration, and reference is provided for application ways and product forms such as preservation or packaging of the berry fruits.

The invention relates to the technical field of organic fertilizers, in particular to a special fruit-promoting and quality-improving organic fertilizer for camellia oleifera trees and a preparation process thereof, and the special fruit-promoting and quality-improving organic fertilizer is prepared from the following raw materials: composted chicken manure, camellia oleifera shell powder, fulvic acid, chitosan oligosaccharide, double-layer coated slow-release particles, a soil conditioner, a microbial agent and zinc sulfate. After the potassium feldspar powder is activated by citric acid, the effectiveness of potassium is remarkably improved, and the inner layer modified whey protein film preferentially obstructs water contact and delays the release of potassium in the initial stage, so that the problem of excessive growth of branches and leaves is avoided; the outer polyethylene glycol-glycerol copolymer film has a temperature-sensitive characteristic, can respond to soil humidity and temperature changes, is gradually degraded and accelerates potassium release, so that the high-potassium requirement in a grease synthesis critical period is met; the double-film barrier structure effectively reduces early loss of potassium, ensures continuous potassium supply in later fruit expansion and grease accumulation stages, and remarkably reduces the risk of fruit drop caused by insufficient potassium.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention discloses a COS-O-luteolin derivative as well as a preparation method and application thereof. The COS-O-luteolin derivative is a compound obtained by modifying hydroxyl of chitosan oligosaccharide with luteolin. The preparation method comprises the following steps: weighing a certain amount of chitosan oligosaccharide, and dissolving the chitosan oligosaccharide in ethanol at 25 DEG C to obtain a chitosan oligosaccharide ethanol solution with the mass concentration of 0.5%; the method comprises the following steps: weighing quantitative luteolin, and dissolving the luteolin in ethanol at 25 DEG C to obtain a luteolin ethanol solution with the mass concentration of 0.5%; the preparation method comprises the following steps: mixing a chitosan oligosaccharide ethanol solution and a luteolin ethanol solution in equal volume, stirring for 2 hours, adjusting the pH value to 7.5, continuously stirring for 20-30 minutes, carrying out rotary evaporation at 45 DEG C to remove a solvent, carrying out vacuum drying at 60 DEG C, crushing and sieving to obtain the COS-O-luteolin derivative which can be applied to preparation of a fresh strawberry postharvest storage agent.

The invention discloses an amino acid water-soluble fertilizer and a preparation process thereof, and relates to the technical field of water-soluble fertilizer preparation. The nutrient solution is prepared from the following raw materials: 35 to 45 parts of compound amino acid, 8 to 12 parts of monopotassium phosphate, 3 to 4 parts of potassium sulfate, 1.5 to 2 parts of disodium calcium ethylenediamine tetraacetate, 1.2 to 1.8 parts of EDTA (Ethylene Diamine Tetraacetic Acid) chelated magnesium, 0.3 to 0.7 part of chelated zinc, 0.1 to 0.3 part of zinc sulfate, 2 to 3 parts of citric acid-monopotassium phosphate buffer solution, 2 to 3 parts of algal polysaccharide, 0.5 to 1 part of mannitol, 2 to 4 parts of potassium fulvate, 1 to 2 parts of chitosan oligosaccharide and 0.5 to 1 part of nano silicon dioxide. 0.5-1 part of polyaspartic acid; 1-2 parts of a dispersing agent; the compound amino acid raw materials comprise 60-70% of fish scale protein water-soluble amino acid and 30-40% of bamboo shoot protein water-soluble amino acid; the amino acid water-soluble fertilizer provided by the invention can effectively improve the nutrient absorption efficiency of crops and promote the growth of the crops.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

The invention belongs to the technical field of chitosan oligosaccharide preparation, and particularly relates to a method for preparing chitosan oligosaccharide based on compound enzyme. According to the method for preparing the chitosan oligosaccharide based on the compound enzyme, a multi-enzyme synergistic system is matched with the design of a specific protective agent, chitosanase and chitinase can cooperatively cut beta-1, 4 glycosidic bonds of chitosan and control molecular weight distribution of products, meanwhile, chitinase can directionally cut the beta-1, 4 bonds at the tail end of chitosan, and the molecular weight distribution of the products is controlled. The papain is used for removing protein impurities in the chitosan to realize accurate control of the polymerization degree. Besides, a specific protective agent matched with the multi-enzyme synergistic system is specifically designed, the specific protective agent and the multi-enzyme composition act together, the molecular weight distribution width can be remarkably reduced, the yield and the DP 4-8 proportion can be increased, meanwhile, physical field ultrasonic assistance is added in the enzymolysis treatment process, and the enzymolysis treatment efficiency is improved. The combination of the substrate and the enzyme is further promoted, and the reaction time is shortened.

A soil conditioner belongs to the technical field of soil improvement, and comprises, by mass, 8-12 parts of chitosan oligosaccharide, 2-3 parts of cellulose oligosaccharide, 5-10 parts of alginate oligosaccharide, 2-6 parts of licorice oligosaccharide, 30-40 parts of humic acid, 5-8 parts of a microbial agent, 10-20 parts of minerals, and 5-10 parts of manganese oxide modified charcoal. According to the invention, oligosaccharide is used as an inducer, humic acid is used for constructing a soil skeleton, microorganisms are used for realizing nutrient circulation, and mineral substances and manganese oxide modified charcoal are used for providing long-acting support, so that biostimulation is used as a guide, the soil structure is adjusted, microbial flora is improved, and the crop yield is increased; the heavy metal polluted soil condition is improved.

Belongs to the technical field of pine wood nematode disease prevention and control, and particularly relates to a preparation method and application of a pyrazole-chitosan oligosaccharide derivative. Chloroacetyl chloride serves as an acylating agent, chitosan oligosaccharide is acetylated, and chloroacetyl chitosan oligosaccharide is generated; then, a pyrazole molecule with nematicidal activity is connected to the Cl site of the chloracetyl chitosan oligosaccharide through ammonolysis reaction, and the novel pyrazole-chitosan oligosaccharide derivative is obtained. The derivative has relatively high contact activity on pine wood nematodes, and LC50 of 24 hours and LC50 of 48 hours are 4.814 mM and 4.061 mM respectively; the chitosan oligosaccharide can remarkably inhibit multiple physiological indexes such as movement, feeding and service life of the pine wood nematodes, and is good in stability, low in molecular weight of the chitosan oligosaccharide, good in water solubility, green and environment-friendly, and the plant toxicity is remarkably reduced. The derivative has a good application prospect in the aspect of controlling the pine wood nematode disease and can be applied to prevention and control of the pine wood nematode disease.

The invention relates to the field of organic compound fertilizers, in particular to a spring rape organic compound fertilizer containing antagonistic bacteria and plant source insect-resistant components and a preparation method thereof.The spring rape organic compound fertilizer is prepared from, by mass, 35-45 parts of humic acid organic particles, 15-25 parts of modified straw carbon, 8-12 parts of seaweed residues, 1-2 parts of matrine extract and 0.3-0.7 part of citral microcapsules. Through the design of a three-layer coating carrier and a biological-chemical synergistic system, the insect pest prevention and control effect in the whole growth period is remarkably improved, yield increase and quality improvement of crops and soil micro-ecology improvement are synchronously achieved, meanwhile, dependence on chemical pesticides and the production cost are greatly reduced, and the method is suitable for large-scale popularization and application. An efficient and environment-friendly solution is provided for organic rape planting, and wide application prospects are achieved.

The invention belongs to the field of pesticide chemistry, and relates to preparation of nano-pesticides, in particular to a nano-pesticide with a two-way conveying function and a preparation method and application of the nano-pesticide. MIL-101 (FeIII, CuII) is used as a pesticide carrier, non-systemic fludioxonil pesticide molecules are loaded in a solution, and then the MIL-101 (CuII, FeIII) MOF nanoparticles loaded with the fludioxonil pesticide are encapsulated with chitosan oligosaccharide in the aqueous solution. The nanometer pesticide preparation prevents fludioxonil pesticide molecules from being released in a solvent in advance, the retention amount of the fludioxonil pesticide on leaf surfaces is increased, the lasting pesticide effect of the fludioxonil pesticide molecules in target crops is improved, the nanometer pesticide preparation is environmentally friendly, and the purposes of disease control, agricultural yield increase and quality improvement are achieved. The nano-carrier provided by the invention can be bidirectionally conveyed in crops through leaf surface pores or root hairs, so that the conveying efficiency of fludioxonil pesticide molecules is improved, and the application scene of the nano-carrier is widened.

The invention discloses an N-acetylglucosamine transferase AcetRS-K23G and an application thereof, and belongs to the technical field of functional enzymes. And the amino acid sequence of the N-acetylglucosamine transferase AcetRS-K23G is as shown in SEQ ID NO. 3. The invention further discloses an application of the N-acetylglucosamine transferase AcetRS-K23G in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRS-K23G disclosed by the invention is obtained by performing mutation modification on the N-acetylglucosamine transferase AcetRS, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 5-7, especially the heptaose, the yield of the heptaose can reach 42.01 mg / L, and the N-acetylglucosamine transferase AcetRS-K23G is an ideal functional enzyme for industrially producing the heptaose. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention provides an antibacterial finishing agent and a preparation method and application thereof, and belongs to the technical field of textile assistants, the antibacterial finishing agent comprises the following raw materials: a chitosan-phytic acid-gallic acid graft copolymer, a citric acid-chitosan oligosaccharide cross-linking agent, sodium polyglutamate, a natural surfactant and water. During application, a padding-baking process is adopted, the finishing agent is diluted into 5-20 g / L working solution, the pH is 6-6.5, two times of padding and two times of rolling are performed, pre-drying is performed for 2-3 min at the temperature of 90-100 DEG C, baking is performed for 2-3 min at the temperature of 150-160 DEG C, an antibacterial layer which is resistant to washing more than or equal to 50 times and has the antibacterial rate more than or equal to 90% can be formed on pure cotton, pure hemp, pure silk and blended fabric thereof, and the finished fabric is soft in hand feeling, free of metal residues and biodegradable.

The invention relates to the field of biological medicine, and discloses eutectic gel based on protein as well as a preparation method and application of the eutectic gel. The eutectic gel based on the protein comprises the following components: gelatin, an antibacterial component and a deep eutectic solvent, and the Gel-COS-Ed eutectic gel comprises the following components: gelatin, chitosan oligosaccharide, emodin, glycerol and betaine. Heating and dissolving gelatin and antibacterial components, and solidifying to obtain gelatin gel; and placing the gelatin gel in a deep eutectic solvent to obtain the eutectic gel based on the protein. The tensile strength of the obtained Gel-COS-Ed eutectic gel is 1.01 MPa, the fracture strain can reach 510%, the Gel-COS-Ed eutectic gel has no obvious phase change in the temperature range of-80 DEG C to 80 DEG C, the bacteriostasis rate on staphylococcus aureus reaches 95.7%, and a new solution is provided for application of wound dressings and biomedical materials.

The invention relates to the technical field of feed production, in particular to an azolla filliculoidas fresh leaf fermented feed which comprises the following components in parts by weight: 70 parts of azolla filliculoidas silage sections, 20 parts of a composite carrier matrix, 5 parts of a natural anti-caking and toxin adsorbent, 7 parts of functional liquid, 10 parts of an energy and flavor synergist and 2 parts of zymophyte. Functional liquid is converted into chitosan oligosaccharide through ultrasonic treatment, and long-chain chitosan is broken into high-solubility chitosan oligosaccharide; through cold plasma activation, an active functional group is grafted on the surface of a chitosan oligosaccharide molecule to form a nanoscale compact protective film; the protective film can inhibit pollution of aerobic mould and saccharomycetes after unsealing, prolong the shelf life of the feed, reduce loss of volatile fatty acids and flavor substances generated by fermentation, and maintain the nutritional value and palatability of the feed.

The invention discloses preparation and application of probiotic double-layer collards based on a molecular armor technology, different double-layer collard systems are formed on the surfaces of probiotics through the molecular armor technology, and specifically, the probiotics are respectively mixed with chitosan oligosaccharide, gelatin and whey protein isolate according to a certain proportion; and self-assembling is carried out under the driving of intermolecular interaction force to form the nano-scale probiotic single-layer collars. Then, pectin is introduced to coat the surfaces of the single-layer collars respectively, and independent packaging of the double-layer collars system on the single probiotics is achieved. The probiotic double-layer collard prepared by the invention not only can promote the growth and reproduction of the probiotics, but also can improve the survival rate and tolerance of the probiotics under the conditions of pasteurization, freeze-thaw cycle, storage at 4 DEG C and in-vitro simulated gastrointestinal environment. More importantly, the preparation method provided by the invention is simple, convenient to operate, relatively low in cost and suitable for industrial large-scale production.

The invention belongs to the technical field of biological organic fertilizers, and provides a multifunctional biological organic fertilizer based on agricultural and forestry wastes and a preparation method thereof. The preparation method comprises the following steps: combining chitosan oligosaccharide molecules with sepiolite, and combining a composite bacterial liquid with a chitosan oligosaccharide-sepiolite composite matrix to obtain a chitosan oligosaccharide-sepiolite-composite probiotic carrier; sodium sulfite and epichlorohydrin are utilized to enable the modified lignin to have higher solubility, and the modified lignin is combined with the nano montmorillonite to form a stable network through glutaraldehyde; converting the carbon-rich biomass into biochar, and combining starch, soybean protein and a mixed trace element solution to obtain a composite synergist; lignocellulose of agricultural and forestry wastes is subjected to structural damage, and cellulose and hemicellulose are degraded and a carbon source is provided for subsequent functional microorganisms under the synergistic effect of a compound enzyme solution; in the composting fermentation process, deep degradation of organic matters is achieved, a sodium alginate solution is sprayed to form a stable nutrient slow-release network, and meanwhile the water retention capacity of the particles is improved.

The invention provides freeze-dried pet food capable of improving intestinal functions of pets and a preparation method of the freeze-dried pet food. Firstly, lecithin and beta-sitosterol are used for constructing lipidosome embedded compound bacteria, and then lactoferrin is combined with embedded compound enzyme to form microspheres; chitosan oligosaccharide is used for connecting compound bacteria liposome and compound enzyme microspheres, the chitosan oligosaccharide is loaded in an alginate gel network to prepare a compound microcapsule, finally raw materials such as yoghourt, camel milk, milk mineral salt and galactooligosaccharide are mixed, and the freeze-dried pet food is prepared through vacuum freeze drying. The sodium alginate composite microcapsule prepared by the invention can greatly improve the stability of probiotics and enzymes, ensure the activity of the probiotics and the enzymes, and improve the palatability of pets to daily ration, increase the food consumption of the pets, promote the digestion and absorption of nutrient substances by the intestinal tracts of the pets and improve the immunity of the pets by virtue of targeted release of the intestinal tracts and synergistic effect of the prebiotics. The intestinal function of the pet is improved, and an efficient and safe solution is provided for the intestinal health of the pet.