564 results about "CHITOSAN OLIGOSACCHARIDE" patented technology

The invention discloses N-acetylglucosamine transferase AcetRL-R349H and an application thereof, and belongs to the technical field of functional enzymes. The amino acid sequence of the N-acetylglucosamine transferase AcetRL-R349H is as shown in SEQ ID NO. 5 (sequence identifier number 5). The invention further discloses the application of the N-acetylglucosamine transferase AcetRL-R349H in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRL-R349H disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRL, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 6, especially (GlcNAc) 6. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention relates to the technical field of cosmetics, and provides a composition containing a dendrobium officinale enzymolysis extract, which is prepared from the following components in percentage by mass: 18 to 22 percent of dendrobium officinale enzymolysis extract, 9 to 11 percent of chitosan oligosaccharide, 6.5 to 7.5 percent of bionic moisturizing enhancer, 0.1 to 0.2 percent of sodium alginate, 0.05 to 0.15 percent of citric acid / sodium citrate buffer pair and 60.4 to 65.4 percent of solvent matrix. By combining low-temperature enzymolysis with a biological purification process, efficient extraction of dendrobium candidum polysaccharides and phenolic substances is realized, dendrophenol oxidation inactivation caused by high temperature can be effectively avoided, hydrochloric acid residues in chitosan oligosaccharide preparation are effectively removed, formula irritation is remarkably reduced, and the dendrobium candidum extract is more suitable for sensitive skin care; the components in the composition have a synergistic effect, the dendrobium officinale enzymolysis extract and the enzymolysis chitosan oligosaccharide form a composite moisturizing network, the moisturizing rate is remarkably increased, the oxidation resistance is remarkably improved, and the composition can be applied to products such as essence, masks and freeze-dried powder.

The invention discloses oligochitosan oligosaccharide, oligochitosan oligosaccharide composite nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of nano materials and food engineering. According to the method disclosed by the invention, the chitosan oligosaccharide with the polymerization degree of 2-6 is prepared by adopting an ultrasonic-microwave synergistic compound enzyme enzymolysis method, so that the yield and the quality of the chitosan oligosaccharide are improved. The chitosan oligosaccharide and zein are assembled to form the chitosan oligosaccharide composite nanoparticles, the chitosan oligosaccharide composite nanoparticles have the advantages of being small in particle size and high in stability, the antioxidant function of the chitosan oligosaccharide is remarkably improved, the preparation method is simple, convenient, economical and practical, and the chitosan oligosaccharide composite nanoparticles can be used as food, health care products and medicine carrier materials and have good market application prospects.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention relates to the technical field of textile material and fiber processing, in particular to a high-elasticity moisture-absorption breathable acetate fabric and a preparation method thereof. The invention discloses a high-elasticity moisture-absorption breathable acetate fabric and a preparation method thereof.The acetate fabric takes a bio-based degradable thermoplastic polyester elastomer as an island and acetate as a sea, a homothermal double-hole structure is embedded in the acetate fabric, and the surface of the acetate fabric is covered with a polyglutamic acid and chitosan oligosaccharide self-repairing hydrophilic layer; the preparation method comprises the following steps: pre-burying alkali-resistant microspheres in a marine facies through solid island-solution sea double-path spinning of a bio-based degradable thermoplastic polyester elastomer and acetate, synchronously forming groove-closed pore double channels through simultaneous heating one-step alkali etching-baking, and finally forming a dynamic reversible hydrogen bond network through polyglutamic acid and chitosan oligosaccharide, so as to obtain the marine-based degradable alkali-resistant hydrogel. A bio-based finishing layer with a self-repairing function is obtained, and the finally obtained fabric has the characteristics of high elasticity, breathability, lasting moisture absorption, greenness and degradability.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention belongs to the technical field of biology, and particularly relates to a composition containing plant-derived exosomes as well as a preparation method and application of the composition. The invention provides a composition containing plant-derived exosomes. The composition is prepared from the following components in parts by mass: 1-60 parts of a plant-derived exosome preparation, 1-60 parts of chitosan oligosaccharide and 1-60 parts of probiotics. According to the composition provided by the invention, the plant-derived exosome, the probiotics and the chitosan oligosaccharide are compounded according to a specific proportion, so that the abundance of effective flora can be increased for a long time, the composition of intestinal flora can be improved, the structural balance of the intestinal flora can be regulated and maintained, the intestinal inflammation can be remarkably inhibited, the intestinal leakage can be improved, and the oxidative stress can be resisted; the repair of damaged intestinal mucosa and the elimination of inflammation are facilitated. The composition is safe and stable in raw materials, free of toxic and side effects and capable of being taken as daily diet.

The invention discloses a composition capable of resisting inflammation, increasing blood oxygen content, inhibiting cancer cell growth and relieving tumor treatment side effects. The composition is specifically prepared from exocarpium citri grandis, ginseng, cortex cinnamomi, ergothioneine, herba houttuyniae, kelp, herba taraxaci, rhizoma curcumae longae, epigallocatechin gallate, naringin, chitosan oligosaccharide, zinc gluconate and taurine. The composition provided by the invention integrates the treatment concepts of softening hardness to dissipate stagnation in the traditional medicine and treating according to the evidence in the modern medicine, and the raw materials are scientific in compatibility, have a synergistic effect and jointly exert the effects of resisting inflammation, improving body immunity, improving the blood oxygen content level of a focus part and the like; the compound has the advantages of well inhibiting growth of cancer cells, inhibiting expression of hypoxia-inducible factors HIF, relieving side effects of tumor treatment and the like, is safe and efficient, and has a wide application prospect.

The invention discloses a method for efficiently promoting slash pine somatic embryo maturation, and relates to the technical field of plant breeding. According to the method, the slash pine embryogenic callus is inoculated into a somatic embryo maturation culture medium containing sulfonatin and / or activated carbon, or PEG8000 and / or chitosan oligosaccharide for somatic embryo induction culture, so that early maturation of slash pine somatic embryos can be remarkably promoted. Results of the invention show that when the concentration of the sulfokine added in the culture medium is 0.5 mg / L, the total number of mature somatic embryos is as high as 109 / dish; when 0.5 mg / L of sulfonatin and 1 mg / L of activated carbon are added at the same time, the number of mature somatic embryos is up to 288 / dish; when 120g / L of PEG 8000 and 6mg / L of chitosan oligosaccharide are added at the same time, the number of mature somatic embryos is up to 123 / dish. In conclusion, the method disclosed by the invention can efficiently promote slash pine somatic embryo maturation, and is high in somatic embryo induction efficiency and strong in practicability.

The invention discloses a bran fermented biological feed and a preparation method thereof, and belongs to the technical field of feeds. The bran fermented biological feed is prepared by uniformly mixing and fermenting the following raw materials: wheat bran, corn flour, soybean meal, calcium hydrogen phosphate, sodium chloride, an enzyme preparation, a water retention setting agent, a mildew preventive and water, wherein the enzyme preparation is prepared from lactobacillus plantarum, bacillus subtilis and saccharomycetes; the mildew preventive is prepared by the following steps: reacting chitosan oligosaccharide with propiolic acid to obtain modified chitosan oligosaccharide, reacting L-cystine with ethyl azidoacetate to obtain an azide compound, and finally reacting the modified chitosan oligosaccharide with the azide compound. The prepared bran fermented biological feed has an excellent mildew-proof effect and a low pulverization rate, and storage and transportation of the feed are facilitated.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The invention relates to the technical field of feed processing, and discloses a lactating sow feed containing fermentation raw materials and a preparation method of the lactating sow feed. A three-layer microcapsule coating auxiliary material is prepared by taking a vitamin premix, an amino acid compound, a compound enzyme preparation and a probiotic agent as core materials through three-layer coating; the basic raw materials comprise corn, wheat, bran, a fermentation raw material, a mineral premix and grease, auxiliary material coating and basic raw material treatment are carried out in parallel in double lines during preparation, and finally, mixing granulation is carried out; the grafted copolymer formed by the amidation reaction of the sodium carboxymethyl cellulose and the chitosan oligosaccharide can cooperate with the sodium alginate to prevent the damage under acidic conditions, and the survival rate of viable bacteria is kept at 85-87% after treatment is carried out for 2 hours under the acidic condition with the pH value of 2.0-3.0; compared with a traditional fermentation raw material coating scheme, the dosage of coating raw materials is reduced by 70% or above; meanwhile, the vitamin premix and the amino acid compound in the auxiliary material can reduce the diarrhea rate by 6% and increase the lactation yield of the sow by 7-18%.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention relates to a chitosan oligosaccharide derivative capable of stabilizing vitamin C in an aqueous solution as well as a preparation method and application of the chitosan oligosaccharide derivative. Different acyl groups are introduced to amino groups of glycine, acylated glycine is coupled to amino groups of chitosan oligosaccharide molecules, the proportion of the two acyl groups is controlled, and the water solubility of the derivative is improved through acetylglycine, so that the vitamin C in the aqueous solution can be stabilized. Through huge hydrophobicity of cinnamyl, attack of part of oxygen molecules to vitamin C molecules is blocked, so that a relatively good protection effect is achieved. The main raw materials are all green and pure natural raw materials, other synthesis raw materials belong to low-toxicity or non-toxic reagents and are cheap and easy to obtain, the reaction is simple, the reaction condition is mild, the yield is high, the reaction can be quantified, industrial mass production is easy to realize, and byproducts involved in the synthesis process can be recycled, so that the preparation cost is greatly saved.

The invention discloses a macrophage-polymer lipid nanoparticle composite drug delivery system, a preparation method thereof and application of the macrophage-polymer lipid nanoparticle composite drug delivery system in cryptococcus infection resistance. According to the drug delivery system, alpha-linolenic acid (ALA), high molecular weight chitosan oligosaccharide (HCOS) and amphotericin B (AmB) are used as raw materials to construct nanoparticles AmB-PLHNs, the nanoparticles are loaded in macrophages through the phagocytosis of the macrophages, and the AmB-PLHNs (at) M1 is constructed. AmB is a common medicine for clinically treating Cn infection, but has the problems of toxic and side effects on kidney and incapability of being transferred into the center. The AmB-PLHNs (at) M1 carries AmB-PLHNs to be transferred into the brain according to the blood-brain barrier penetrating characteristic of M1 type macrophages, and the brain entering efficiency of the medicine is improved. By combining the phagocytosis of macrophages on cryptococcus, the inflammation immune regulation function of the macrophages and the bacteriostatic action of the drug-loaded nanoparticles, the drug-loaded nano-particles are used for treating Cn central infection, and the toxic and side effects of AmB are reduced.

The invention discloses a preparation method of a chitosan oligosaccharide-anthocyanin nanocomposite. The preparation method comprises the following steps: dissolving purple corn anthocyanin in distilled water; dissolving chitosan oligosaccharide in distilled water; the method comprises the following steps: adding an organic solvent into a reaction kettle, reacting at room temperature in a dark place for 4-48 hours, transferring a mixed solution into a centrifugal tube, centrifuging for 10-30 minutes to separate out nano-composite precipitates and uncombined supernatant, and then adding ultrapure water for repeated centrifugation. And finally, carrying out freeze drying treatment on the precipitate to obtain the final chitosan oligosaccharide-anthocyanin nano-composite. The nano-composite prepared by the method has high stability, good dispersibility and enhanced biological activity, and provides a new way for wide application of chitosan oligosaccharide and anthocyanin in the fields of food, cosmetics, biological medicine and the like.

The invention discloses a fresh-cut eggplant anti-browning and fresh-keeping method based on composite plant extract synergistic heat treatment, and the method comprises the following steps: S1, preparing a composite plant extract comprising the following components: a green tea extract, a rosemary extract, a licorice extract, trehalose, chitosan oligosaccharide, curcumin microcapsules, soybean lecithin, Tween, and the balance of pure water; s2, the fresh-cut eggplants are soaked in a composite plant extracting solution, gradient heat treatment is carried out, and the gradient heat treatment comprises the following three stages of a heat activation permeation stage, a cooling stabilization stage and a cold shock fixation stage; and S3, slightly draining the treated fresh-cut eggplants, and packaging with a preservative film. Through the synergistic effect of various plant extracts and an elaborately-designed gradient heat treatment process, effective inhibition of browning of the fresh-cut eggplants is achieved, the fresh-keeping period is remarkably prolonged, good quality can be kept for 10 days or above under the condition of 4 + / -1 DEG C, and the fresh-cut eggplant fresh-keeping method has a good application prospect.

The invention relates to a composite blocking remover for an oil field and a preparation method thereof, and belongs to the technical field of oil exploitation. The composite blocking remover is prepared from the following components: 18 to 22 weight percent of composite acid, 2.7 to 3.5 weight percent of a modified chitosan oligosaccharide synergist, 0.35 to 0.52 weight percent of a surfactant, 0.1 to 0.12 weight percent of a corrosion inhibitor and the balance of water, according to the modified chitosan oligosaccharide synergist, chitosan oligosaccharide is used as a rigid base chain, a supporting effect is provided, double chains are prevented from being intertwined in a water-based environment, a polythioamide chain containing an alkyl quaternary ammonium structure is used as a functional auxiliary chain, redeposition of dissolved matter is relieved through gel breaking-chelation integration, meanwhile, a chain structure forms an adhesive tape effect, and the synergistic effect is achieved. And the blockage removing driving force is increased, so that efficient blockage removing is realized.

The invention discloses a catechin-chitosan oligosaccharide-nano selenium composite material as well as a preparation method and application thereof, and belongs to the technical field of biomass nano materials. The preparation method of the catechin-chitosan oligosaccharide-nano selenium composite material comprises the following steps: firstly, mixing an aqueous solution of sodium selenite and an aqueous solution of epigallocatechin gallate according to the molar ratio of 1: (10-25), and stirring; the catechin-chitosan oligosaccharide-nano-selenium composite material is prepared by adding the catechin into sodium selenite, then adding chitosan oligosaccharide and water, and reacting to obtain the catechin-chitosan oligosaccharide-nano-selenium composite material, the final concentration of the sodium selenite in the reaction system is 1mM, and the final concentration of the chitosan oligosaccharide in percentage by mass is 1-2%. The composite material provided by the invention has relatively excellent antibacterial activity, is easy to clean, natural and environment-friendly, the preservation effect of the composite material applied to the berry fruits is superior to the effect of chitosan under the same concentration, and reference is provided for application ways and product forms such as preservation or packaging of the berry fruits.

The invention relates to the technical field of organic fertilizers, in particular to a special fruit-promoting and quality-improving organic fertilizer for camellia oleifera trees and a preparation process thereof, and the special fruit-promoting and quality-improving organic fertilizer is prepared from the following raw materials: composted chicken manure, camellia oleifera shell powder, fulvic acid, chitosan oligosaccharide, double-layer coated slow-release particles, a soil conditioner, a microbial agent and zinc sulfate. After the potassium feldspar powder is activated by citric acid, the effectiveness of potassium is remarkably improved, and the inner layer modified whey protein film preferentially obstructs water contact and delays the release of potassium in the initial stage, so that the problem of excessive growth of branches and leaves is avoided; the outer polyethylene glycol-glycerol copolymer film has a temperature-sensitive characteristic, can respond to soil humidity and temperature changes, is gradually degraded and accelerates potassium release, so that the high-potassium requirement in a grease synthesis critical period is met; the double-film barrier structure effectively reduces early loss of potassium, ensures continuous potassium supply in later fruit expansion and grease accumulation stages, and remarkably reduces the risk of fruit drop caused by insufficient potassium.

The invention belongs to the technical field of agriculture, and relates to an Ectoin-containing salt-alkali-resistant, high-temperature-resistant and growth-promoting composition as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 12 to 15 parts of aloin, 6 to 10 parts of Ectoin, 10 to 15 parts of seaweed extract, 10 to 15 parts of gamma-aminobutyric acid, 3 to 4 parts of polyvinyl alcohol, 2 to 3 parts of sodium dodecyl benzene sulfonate, 3 to 4 parts of tween-20, 5 to 8 parts of glyceryl alcohol, 5 to 8 parts of ethyl salicylate, 5 to 10 parts of chitosan oligosaccharide and 45 to 50 parts of water. The composition disclosed by the invention can be used for remarkably improving the stress resistance of crops, relieving the membrane damage of saline-alkali and high-temperature stress to the crops, enhancing the activity of antioxidant enzymes in crop leaves under the saline-alkali and high-temperature stress and promoting the growth of the crops under the saline-alkali and high-temperature stress conditions.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention discloses a planting method of selenium-rich cherries. The planting method comprises the following steps that selenium-containing fertilizer is applied to soil in a flushing mode when tree bodies germinate at the end stage of a dormancy period; in the flowering stage, nitrogen-phosphorus-potassium balanced fertilizer is applied, and the synergist composition and the selenium-enriched nutritional agent are sprayed on leaf surfaces at intervals; and spraying the synergist composition and the selenium-rich nutritional agent on leaf surfaces at intervals for multiple times in a fruit swelling period. The synergist composition disclosed by the invention is prepared from the following components: polyoxyethylene fatty alcohol ether, polyether modified organic silicon, natural plant saponin, chitosan oligosaccharide, alginic acid, hydrolyzed collagen and the like. The selenium content of the cherry fruits cultivated by the method can reach 0.078-0.082 mg / kg, after the cherry fruits are stored for 15 days, the hardness of the fruits is maintained at 65-68.5 N / cm < 2 >, the weight loss rate is controlled at 4.0-4.5%, the rotting rate is controlled at 3.2-3.8%, the retention rate of vitamin C reaches 83-86%, and the storage period is prolonged by 3-4 days compared with that of conventional cultivation. The method is easy to operate, low in cost and high in applicability, and the quality and storage performance of the cherry fruits can be remarkably improved.

The invention discloses a COS-O-luteolin derivative as well as a preparation method and application thereof. The COS-O-luteolin derivative is a compound obtained by modifying hydroxyl of chitosan oligosaccharide with luteolin. The preparation method comprises the following steps: weighing a certain amount of chitosan oligosaccharide, and dissolving the chitosan oligosaccharide in ethanol at 25 DEG C to obtain a chitosan oligosaccharide ethanol solution with the mass concentration of 0.5%; the method comprises the following steps: weighing quantitative luteolin, and dissolving the luteolin in ethanol at 25 DEG C to obtain a luteolin ethanol solution with the mass concentration of 0.5%; the preparation method comprises the following steps: mixing a chitosan oligosaccharide ethanol solution and a luteolin ethanol solution in equal volume, stirring for 2 hours, adjusting the pH value to 7.5, continuously stirring for 20-30 minutes, carrying out rotary evaporation at 45 DEG C to remove a solvent, carrying out vacuum drying at 60 DEG C, crushing and sieving to obtain the COS-O-luteolin derivative which can be applied to preparation of a fresh strawberry postharvest storage agent.

The invention discloses an amino acid water-soluble fertilizer and a preparation process thereof, and relates to the technical field of water-soluble fertilizer preparation. The nutrient solution is prepared from the following raw materials: 35 to 45 parts of compound amino acid, 8 to 12 parts of monopotassium phosphate, 3 to 4 parts of potassium sulfate, 1.5 to 2 parts of disodium calcium ethylenediamine tetraacetate, 1.2 to 1.8 parts of EDTA (Ethylene Diamine Tetraacetic Acid) chelated magnesium, 0.3 to 0.7 part of chelated zinc, 0.1 to 0.3 part of zinc sulfate, 2 to 3 parts of citric acid-monopotassium phosphate buffer solution, 2 to 3 parts of algal polysaccharide, 0.5 to 1 part of mannitol, 2 to 4 parts of potassium fulvate, 1 to 2 parts of chitosan oligosaccharide and 0.5 to 1 part of nano silicon dioxide. 0.5-1 part of polyaspartic acid; 1-2 parts of a dispersing agent; the compound amino acid raw materials comprise 60-70% of fish scale protein water-soluble amino acid and 30-40% of bamboo shoot protein water-soluble amino acid; the amino acid water-soluble fertilizer provided by the invention can effectively improve the nutrient absorption efficiency of crops and promote the growth of the crops.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

The invention belongs to the technical field of chitosan oligosaccharide preparation, and particularly relates to a method for preparing chitosan oligosaccharide based on compound enzyme. According to the method for preparing the chitosan oligosaccharide based on the compound enzyme, a multi-enzyme synergistic system is matched with the design of a specific protective agent, chitosanase and chitinase can cooperatively cut beta-1, 4 glycosidic bonds of chitosan and control molecular weight distribution of products, meanwhile, chitinase can directionally cut the beta-1, 4 bonds at the tail end of chitosan, and the molecular weight distribution of the products is controlled. The papain is used for removing protein impurities in the chitosan to realize accurate control of the polymerization degree. Besides, a specific protective agent matched with the multi-enzyme synergistic system is specifically designed, the specific protective agent and the multi-enzyme composition act together, the molecular weight distribution width can be remarkably reduced, the yield and the DP 4-8 proportion can be increased, meanwhile, physical field ultrasonic assistance is added in the enzymolysis treatment process, and the enzymolysis treatment efficiency is improved. The combination of the substrate and the enzyme is further promoted, and the reaction time is shortened.

A soil conditioner belongs to the technical field of soil improvement, and comprises, by mass, 8-12 parts of chitosan oligosaccharide, 2-3 parts of cellulose oligosaccharide, 5-10 parts of alginate oligosaccharide, 2-6 parts of licorice oligosaccharide, 30-40 parts of humic acid, 5-8 parts of a microbial agent, 10-20 parts of minerals, and 5-10 parts of manganese oxide modified charcoal. According to the invention, oligosaccharide is used as an inducer, humic acid is used for constructing a soil skeleton, microorganisms are used for realizing nutrient circulation, and mineral substances and manganese oxide modified charcoal are used for providing long-acting support, so that biostimulation is used as a guide, the soil structure is adjusted, microbial flora is improved, and the crop yield is increased; the heavy metal polluted soil condition is improved.

Belongs to the technical field of pine wood nematode disease prevention and control, and particularly relates to a preparation method and application of a pyrazole-chitosan oligosaccharide derivative. Chloroacetyl chloride serves as an acylating agent, chitosan oligosaccharide is acetylated, and chloroacetyl chitosan oligosaccharide is generated; then, a pyrazole molecule with nematicidal activity is connected to the Cl site of the chloracetyl chitosan oligosaccharide through ammonolysis reaction, and the novel pyrazole-chitosan oligosaccharide derivative is obtained. The derivative has relatively high contact activity on pine wood nematodes, and LC50 of 24 hours and LC50 of 48 hours are 4.814 mM and 4.061 mM respectively; the chitosan oligosaccharide can remarkably inhibit multiple physiological indexes such as movement, feeding and service life of the pine wood nematodes, and is good in stability, low in molecular weight of the chitosan oligosaccharide, good in water solubility, green and environment-friendly, and the plant toxicity is remarkably reduced. The derivative has a good application prospect in the aspect of controlling the pine wood nematode disease and can be applied to prevention and control of the pine wood nematode disease.